This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Keleher, C A
Right arrow Articles by Johnson, A D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Keleher, C A
Right arrow Articles by Johnson, A D

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 November; 9(11): 5228-5230

Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1.

C A Keleher, S Passmore and A D Johnson

Department of Biochemistry and Biophysics, University of California, San Francisco 94143.

ABSTRACT

To bring about repression of a family fo genes in Saccharomyces cerevisiae called the a-specific genes, two transcriptional regulatory proteins, alpha 2 and GRM (general regulator of matin type), bind cooperatively to an operator found upstream of each a-specific gene. To date, GRM has been defined only biochemically. In this communication we show that the product of a single yeast gene (MCM1) is sufficient to bind cooperatively with alpha 2 to the operator. We also show that antiserum raised against the MCM1 gene product recognizes GRM from yeast cells. These results, in combination with previous observations, provide strong evidence that MCM1 encodes the GRM activity.

Mol Cell Biol. 1989 November; 9(11): 5228-5230




This article has been cited by other articles:

  • Rutherford, J. C., Chua, G., Hughes, T., Cardenas, M. E., Heitman, J. (2008). A Mep2-dependent Transcriptional Profile Links Permease Function to Gene Expression during Pseudohyphal Growth in Saccharomyces cerevisiae. Mol. Biol. Cell 19: 3028-3039 [Abstract] [Full Text]  
  • Polish, J. A., Kim, J.-H., Johnston, M. (2005). How the Rgt1 Transcription Factor of Saccharomyces cerevisiae Is Regulated by Glucose. Genetics 169: 583-594 [Abstract] [Full Text]  
  • Carr, E. A., Mead, J., Vershon, A. K. (2004). {alpha}1-induced DNA bending is required for transcriptional activation by the Mcm1-{alpha}1 complex. Nucleic Acids Res 32: 2298-2305 [Abstract] [Full Text]  
  • Soll, D. R., Lockhart, S. R., Zhao, R. (2003). Relationship between Switching and Mating in Candida albicans. Eukaryot Cell 2: 390-397 [Full Text]  
  • Lim, F.-L., Hayes, A., West, A. G., Pic-Taylor, A., Darieva, Z., Morgan, B. A., Oliver, S. G., Sharrocks, A. D. (2003). Mcm1p-Induced DNA Bending Regulates the Formation of Ternary Transcription Factor Complexes. Mol. Cell. Biol. 23: 450-461 [Abstract] [Full Text]  
  • Mead, J., Bruning, A. R., Gill, M. K., Steiner, A. M., Acton, T. B., Vershon, A. K. (2002). Interactions of the Mcm1 MADS Box Protein with Cofactors That Regulate Mating in Yeast. Mol. Cell. Biol. 22: 4607-4621 [Abstract] [Full Text]  
  • Mai, B., Miles, S., Breeden, L. L. (2002). Characterization of the ECB Binding Complex Responsible for the M/G1-Specific Transcription of CLN3 and SWI4. Mol. Cell. Biol. 22: 430-441 [Abstract] [Full Text]  
  • Teng, Y., Yu, S., Waters, R. (2001). The mapping of nucleosomes and regulatory protein binding sites at the Saccharomyces cerevisiae MFA2 gene: a high resolution approach. Nucleic Acids Res 29: e64-e64 [Abstract] [Full Text]  
  • Nasiadka, A, Grill, A, Krause, H. (2000). Mechanisms regulating target gene selection by the homeodomain-containing protein Fushi tarazu. Development 127: 2965-2976 [Abstract]  
  • Jin, Y., Zhong, H., Vershon, A. K. (1999). The Yeast a1 and alpha 2 Homeodomain Proteins Do Not Contribute Equally to Heterodimeric DNA Binding. Mol. Cell. Biol. 19: 585-593 [Abstract] [Full Text]  
  • Nicol, R., Stavnezer, E. (1998). Transcriptional Repression by v-Ski and c-Ski Mediated by a Specific DNA Binding Site. J. Biol. Chem. 273: 3588-3597 [Abstract] [Full Text]  
  • Szeto, L., Fafalios, M. K., Zhong, H., Vershon, A. K., Broach, J. R. (1997). alpha 2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III. Genes Dev. 11: 1899-1911 [Abstract] [Full Text]  
  • Zhong, H., Vershon, A. K. (1997). The Yeast Homeodomain Protein MATalpha 2 Shows Extended DNA binding Specificity in Complex with Mcm1. J. Biol. Chem. 272: 8402-8409 [Abstract] [Full Text]  
  • Jin, Y., Mead, J., Li, T., Wolberger, C., Vershon, A. K. (1995). Altered DNA Recognition and Bending by Insertions in the alpha2 Tail of the Yeast a1/alpha2 Homeodomai n Heterodimer. Science 270: 290-293 [Abstract]  
  • Andrews, B. J., Donoviel, M. S. (1995). A Heterodimeric Transcriptional Repressor Becomes Crystal Clear. Science 270: 251-251 [Abstract]  
  • Komachi, K, Redd, M J, Johnson, A D (1994). The WD repeats of Tup1 interact with the homeo domain protein alpha 2.. Genes Dev. 8: 2857-2867 [Abstract]  
  • Cooper, J P, Roth, S Y, Simpson, R T (1994). The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.. Genes Dev. 8: 1400-1410 [Abstract]  
  • Edmondson, D., Lyons, G., Martin, J., Olson, E. (1994). Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis. Development 120: 1251-1263 [Abstract]  
  • Chan, S K, Mann, R S (1993). The segment identity functions of Ultrabithorax are contained within its homeo domain and carboxy-terminal sequences.. Genes Dev. 7: 796-811 [Abstract]  
  • Ip, Y T, Park, R E, Kosman, D, Bier, E, Levine, M (1992). The dorsal gradient morphogen regulates stripes of rhomboid expression in the presumptive neuroectoderm of the Drosophila embryo.. Genes Dev. 6: 1728-1739 [Abstract]  
  • Hardy, C F, Sussel, L, Shore, D (1992). A RAP1-interacting protein involved in transcriptional silencing and telomere length regulation.. Genes Dev. 6: 801-814 [Abstract]  
  • Roth, S Y, Shimizu, M, Johnson, L, Grunstein, M, Simpson, R T (1992). Stable nucleosome positioning and complete repression by the yeast alpha 2 repressor are disrupted by amino-terminal mutations in histone H4.. Genes Dev. 6: 411-425 [Abstract]  
  • Harley, V., Jackson, D., Hextall, P., Hawkins, JR, Berkovitz, G., Sockanathan, S, Lovell-Badge, R, Goodfellow, P. (1992). DNA binding activity of recombinant SRY from normal males and XY females. Science 255: 453-456 [Abstract]  
  • Pollock, R, Treisman, R (1991). Human SRF-related proteins: DNA-binding properties and potential regulatory targets.. Genes Dev. 5: 2327-2341 [Abstract]  
  • Sengupta, P, Cochran, B H (1991). MAT alpha 1 can mediate gene activation by a-mating factor.. Genes Dev. 5: 1924-1934 [Abstract]  
  • Christ, C, Tye, B K (1991). Functional domains of the yeast transcription/replication factor MCM1.. Genes Dev. 5: 751-763 [Abstract]  
  • Phillips, C L, Vershon, A K, Johnson, A D, Dahlquist, F W (1991). Secondary structure of the homeo domain of yeast alpha 2 repressor determined by NMR spectroscopy.. Genes Dev. 5: 764-772 [Abstract]  
  • Mathias, J. R., Zhong, H., Jin, Y., Vershon, A. K. (2001). Altering the DNA-binding Specificity of the Yeast Matalpha 2 Homeodomain Protein. J. Biol. Chem. 276: 32696-32703 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»