This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hurd, H K Articles by Roberts, J W Search for Related Content PubMed PubMed Citation Articles by Hurd, H K Articles by Roberts, J W

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 December; 9(12): 5359-5372

Upstream regulatory sequences of the yeast RNR2 gene include a repression sequence and an activation site that binds the RAP1 protein.

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.

ABSTRACT

The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation of RNR2, we introduced deletions upstream of the transcription start site. Sequences required for induction were contained within a 200-base-pair region that could confer methyl methanesulfonate inducibility on the heterologous CYC1 promoter. This region contained a repression sequence and at least two positive activation sites. One of these activation sites bound RAP1, a protein known to associate with mating-type silencers and the upstream activation sequences of a number of genes. The behavior of deletions of the repression sequence suggests that induction of RNR2 may occur, at least in part, through relief of repression.

This article has been cited by other articles:

• Cohen, Y., Dardalhon, M., Averbeck, D. (2002). Homologous recombination is essential for RAD51 up-regulation in Saccharomyces cerevisiae following DNA crosslinking damage. Nucleic Acids Res 30: 1224-1232 [Abstract] [Full Text]  
• Fikus, M. U., Mieczkowski, P. A., Koprowski, P., Rytka, J., ledziewska-Gójska, E., Ciela, Z. (2000). The Product of the DNA Damage-Inducible Gene of Saccharomyces cerevisiae, DIN7, Specifically Functions in Mitochondria. Genetics 154: 73-81 [Abstract] [Full Text]  
• Ho, Y., Mason, S., Kobayashi, R., Hoekstra, M., Andrews, B. (1997). Role of the casein kinase I isoform, Hrr25, and the cell cycle-regulatory transcription factor, SBF, in the transcriptional response to DNA damage in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 94: 581-586 [Abstract] [Full Text]  
• Kurtz, S, Shore, D (1991). RAP1 protein activates and silences transcription of mating-type genes in yeast.. Genes Dev. 5: 616-628 [Abstract]