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Mol Cell Biol. 1989 December; 9(12): 5434-5439

The G protein alpha o subunit alters morphology, growth kinetics, and phospholipid metabolism of somatic cells.

D B Bloch, J V Bonventre, E J Neer and J G Seidman

Department of Genetics, Massachusetts General Hospital, Boston 02114.

ABSTRACT

The physiological role of the alpha o subunit of guanine nucleotide-binding (G) protein was investigated with a murine adrenal cell line (Y1) transfected with a rat alpha o cDNA cloned in a retroviral expression vector. The parental cell line lacked detectable alpha o subunit. Expression of the alpha o cDNA in transfected cell lines was confirmed by Western blot (immunoblot) analysis. The rat alpha o subunit interacted with murine beta and gamma subunits and associated with cell membranes. Y1 cells containing large amounts of alpha o subunit had altered cellular morphology and reduced rate of cell division. In addition, GTP-gamma S-stimulated release of arachidonic acid from these cells was significantly increased compared with that in control cells. The alpha o subunit appears directly or indirectly to regulate cellular proliferation, morphology, and phospholipid metabolism.

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Mol Cell Biol. 1989 December; 9(12): 5434-5439

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