This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bloch, D B Articles by Seidman, J G Search for Related Content PubMed PubMed Citation Articles by Bloch, D B Articles by Seidman, J G

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 December; 9(12): 5434-5439

The G protein alpha o subunit alters morphology, growth kinetics, and phospholipid metabolism of somatic cells.

Department of Genetics, Massachusetts General Hospital, Boston 02114.

ABSTRACT

The physiological role of the alpha o subunit of guanine nucleotide-binding (G) protein was investigated with a murine adrenal cell line (Y1) transfected with a rat alpha o cDNA cloned in a retroviral expression vector. The parental cell line lacked detectable alpha o subunit. Expression of the alpha o cDNA in transfected cell lines was confirmed by Western blot (immunoblot) analysis. The rat alpha o subunit interacted with murine beta and gamma subunits and associated with cell membranes. Y1 cells containing large amounts of alpha o subunit had altered cellular morphology and reduced rate of cell division. In addition, GTP-gamma S-stimulated release of arachidonic acid from these cells was significantly increased compared with that in control cells. The alpha o subunit appears directly or indirectly to regulate cellular proliferation, morphology, and phospholipid metabolism.

This article has been cited by other articles:

• Strittmatter, S., Valenzuela, D, Fishman, M. (1994). An amino-terminal domain of the growth-associated protein GAP-43 mediates its effects on filopodial formation and cell spreading. J. Cell Sci. 107: 195-204 [Abstract]  
• Gupta, S., Diez, E, Heasley, L., Osawa, S, Johnson, G. (1990). A G protein mutant that inhibits thrombin and purinergic receptor activation of phospholipase A2. Science 249: 662-666 [Abstract]