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The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor.

MEDIGEN Project, Department of Medicine, Stanford University School of Medicine, Palo Alto, California.

ABSTRACT

The c-fos serum response element (SRE) and a sarcomeric actin promoter element (CArG box) are similar in sequence and are recognized, respectively, by the serum response factor (SRF) and the CArG-binding factor (CBF). Although the transcriptional controls for the c-fos and sarcomeric actin genes are rather different, SRF and CBF have been found to be indistinguishable by all criteria tested. They exhibited similar chromatographic properties, sedimentation rates, and temperature stabilities. In mobility shift assays, the SRE competed more strongly than the actin CArG box for formation of either the SRF-SRE or the CBF-CArG complex. The symmetric inverted repeat of the left side of the Xenopus cytoskeletal actin SRE also competed, even more strongly, for each complex. The site-specific binding of each protein was inhibited both by orthophenanthroline, whose effects were reversed by zinc addition, and by treatment with potato acid phosphatase. Furthermore, immune serum raised against the c-fos SRF also recognized the actin CBF. We discuss how transcriptional control of these diverse genes might be obtained with a single similar factor.

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• WEI, L., WANG, L., CARSON, J. A., AGAN, J. E., IMANAKA-YOSHIDA, K., SCHWARTZ, R. J. (2001). {beta}1 integrin and organized actin filaments facilitate cardiomyocyte-specific RhoA-dependent activation of the skeletal {alpha}-actin promoter. FASEB J. 15: 785-796 [Abstract] [Full Text]  
• Morin, S., Paradis, P., Aries, A., Nemer, M. (2001). Serum Response Factor-GATA Ternary Complex Required for Nuclear Signaling by a G-Protein-Coupled Receptor. Mol. Cell. Biol. 21: 1036-1044 [Abstract] [Full Text]  
• Wei, L., Zhou, W., Wang, L., Schwartz, R. J. (2000). beta 1-Integrin and PI 3-kinase regulate RhoA-dependent activation of skeletal alpha -actin promoter in myoblasts. Am. J. Physiol. Heart Circ. Physiol. 278: H1736-H1743 [Abstract] [Full Text]  
• Spencer, J. A., Baron, M. H., Olson, E. N. (1999). Cooperative Transcriptional Activation by Serum Response Factor and the High Mobility Group Protein SSRP1. J. Biol. Chem. 274: 15686-15693 [Abstract] [Full Text]  
• Biesiada, E., Hamamori, Y., Kedes, L., Sartorelli, V. (1999). Myogenic Basic Helix-Loop-Helix Proteins and Sp1 Interact as Components of a Multiprotein Transcriptional Complex Required for Activity of the Human Cardiac alpha -Actin Promoter. Mol. Cell. Biol. 19: 2577-2584 [Abstract] [Full Text]  
• Galvagni, F., Cartocci, E., Oliviero, S. (1998). The Dystrophin Promoter Is Negatively Regulated by YY1 in Undifferentiated Muscle Cells. J. Biol. Chem. 273: 33708-33713 [Abstract] [Full Text]  
• Wei, L., Zhou, W., Croissant, J. D., Johansen, F.-E., Prywes, R., Balasubramanyam, A., Schwartz, R. J. (1998). RhoA Signaling via Serum Response Factor Plays an Obligatory Role in Myogenic Differentiation. J. Biol. Chem. 273: 30287-30294 [Abstract] [Full Text]  
• Chin, M. T., Pellacani, A., Wang, H., Lin, S. S. J., Jain, M. K., Perrella, M. A., Lee, M.-E. (1998). Enhancement of Serum-response Factor-dependent Transcription and DNA Binding by the Architectural Transcription Factor HMG-I(Y). J. Biol. Chem. 273: 9755-9760 [Abstract] [Full Text]  
• Zilberman, A., Dave, V., Miano, J., Olson, E. N., Periasamy, M. (1998). Evolutionarily Conserved Promoter Region Containing CArG*-Like Elements Is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression. Circ. Res. 82: 566-575 [Abstract] [Full Text]  
• Obata, H., Hayashi, K.'i., Nishida, W., Momiyama, T., Uchida, A., Ochi, T., Sobue, K. (1997). Smooth Muscle Cell Phenotype-dependent Transcriptional Regulation of the alpha 1 Integrin Gene. J. Biol. Chem. 272: 26643-26651 [Abstract] [Full Text]  
• Belaguli, N. S., Schildmeyer, L. A., Schwartz, R. J. (1997). Organization and Myogenic Restricted Expression of the Murine Serum Response Factor Gene. A ROLE FOR AUTOREGULATION. J. Biol. Chem. 272: 18222-18231 [Abstract] [Full Text]  
• Chen, C.-Y., Schwartz, R. J. (1997). Competition Between Negative Acting YY1 versus Positive Acting Serum Response Factor and Tinman Homologue Nkx-2.5 Regulates Cardiac {{alpha}}-Actin Promoter Activity. Mol. Endocrinol. 11: 812-822 [Abstract] [Full Text]  
• Sepulveda, A. R., Huang, S. L., Lebovitz, R. M., Lieberman, M. W. (1997). A 346-Base Pair Region of the Mouse gamma -Glutamyl Transpeptidase Type II Promoter Contains Sufficient Cis-acting Elements for Kidney-restricted Expression in Transgenic Mice. J. Biol. Chem. 272: 11959-11967 [Abstract] [Full Text]  
• White, S. L., Low, R. B. (1996). Identification of Promoter Elements Involved in Cell-specific Regulation of Rat Smooth Muscle Myosin Heavy Chain Gene Transcription. J. Biol. Chem. 271: 15008-15017 [Abstract] [Full Text]  
• Groisman, R., Masutani, H., Leibovitch, M.-P., Robin, P., Soudant, I., Trouche, D., Harel-Bellan, A. (1996). Physical Interaction between the Mitogen-responsive Serum Response Factor and Myogenic Basic-Helix-Loop-Helix Proteins. J. Biol. Chem. 271: 5258-5264 [Abstract] [Full Text]  
• Sprenkle, A. B., Murray, S. F., Glembotski, C. C. (1995). Involvement of Multiple cis Elements in Basal- and {alpha}-Adrenergic Agonist–Inducible Atrial Natriuretic Factor Transcription : Roles for Serum Response Elements and an SP-1–Like Element. Circ. Res. 77: 1060-1069 [Abstract] [Full Text]  
• Shimizu, R. T., Blank, R. S., Jervis, R., Lawrenz-Smith, S. C., Owens, G. K. (1995). The Smooth Muscle alpha-Actin Gene Promoter Is Differentially Regulated in Smooth Muscle versus Non-smooth Muscle Cells. J. Biol. Chem. 270: 7631-7643 [Abstract] [Full Text]  
• Karns, L. R., Kariya, K.-i., Simpson, P. C. (1995). M-CAT, CArG, and Sp1 Elements Are Required for alpha(1)-Adrenergic Induction of the Skeletal alpha-Actin Promoter during Cardiac Myocyte Hypertrophy. J. Biol. Chem. 270: 410-417 [Abstract] [Full Text]  
• Goto, K, Meyerowitz, E M (1994). Function and regulation of the Arabidopsis floral homeotic gene PISTILLATA.. Genes Dev. 8: 1548-1560 [Abstract]  
• Yu, Y T, Breitbart, R E, Smoot, L B, Lee, Y, Mahdavi, V, Nadal-Ginard, B (1992). Human myocyte-specific enhancer factor 2 comprises a group of tissue-restricted MADS box transcription factors.. Genes Dev. 6: 1783-1798 [Abstract]  
• Pollock, R, Treisman, R (1991). Human SRF-related proteins: DNA-binding properties and potential regulatory targets.. Genes Dev. 5: 2327-2341 [Abstract]  
• Metz, R, Ziff, E (1991). cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.. Genes Dev. 5: 1754-1766 [Abstract]  
• Ma, H, Yanofsky, M F, Meyerowitz, E M (1991). AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes.. Genes Dev. 5: 484-495 [Abstract]  
• Sartorelli, V, Webster, K A, Kedes, L (1990). Muscle-specific expression of the cardiac alpha-actin gene requires MyoD1, CArG-box binding factor, and Sp1.. Genes Dev. 4: 1811-1822 [Abstract]  
• Rivera, V M, Sheng, M, Greenberg, M E (1990). The inner core of the serum response element mediates both the rapid induction and subsequent repression of c-fos transcription following serum stimulation.. Genes Dev. 4: 255-268 [Abstract]  
• Carson, J. A., Fillmore, R. A., Schwartz, R. J., Zimmer, W. E. (2000). The Smooth Muscle gamma -Actin Gene Promoter Is a Molecular Target for the Mouse bagpipe Homologue, mNkx3-1, and Serum Response Factor. J. Biol. Chem. 275: 39061-39072 [Abstract] [Full Text]  
• Gupta, M., Kogut, P., Davis, F. J., Belaguli, N. S., Schwartz, R. J., Gupta, M. P. (2001). Physical Interaction between the MADS Box of Serum Response Factor and the TEA/ATTS DNA-binding Domain of Transcription Enhancer Factor-1. J. Biol. Chem. 276: 10413-10422 [Abstract] [Full Text]  
• Chang, P. S., Li, L., McAnally, J., Olson, E. N. (2001). Muscle Specificity Encoded by Specific Serum Response Factor-binding Sites. J. Biol. Chem. 276: 17206-17212 [Abstract] [Full Text]  
• Davis, F. J., Gupta, M., Pogwizd, S. M., Bacha, E., Jeevanandam, V., Gupta, M. P. (2002). Increased expression of alternatively spliced dominant-negative isoform of SRF in human failing hearts. Am. J. Physiol. Heart Circ. Physiol. 282: H1521-H1533 [Abstract] [Full Text]