This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rai, R Articles by Cooper, T G Search for Related Content PubMed PubMed Citation Articles by Rai, R Articles by Cooper, T G

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 February; 9(2): 602-608

Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae.

Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.

ABSTRACT

DAL5 is a constitutively expressed allantoin system gene whose product is required for allantoate transport. Its simple pattern of expression prompted us to use this gene for identifying the element(s) that mediates transcriptional activation of allantoin system genes. Deletion analysis of the DAL5 5'-flanking sequences resulted in identification of two small regions required for DAL5 expression. Analysis of these two regions with synthetic oligonucleotides localized the sequences supporting transcriptional activation to two DNA fragments of 10 to 12 base pairs, each containing one copy of the pentanucleotide 5'-GATAA-3'. The 5'-flanking region of DAL5 contained eight copies of this sequence. Synthetic constructions containing single copies of these fragments were unable to support transcriptional activation, while those containing two or more copies supported high-level activation. The 5'-GATAA-3' sequence was also found beneath the footprint of a DNA-binding protein. These observations are consistent with the suggestion that DNA fragments containing the sequence 5'-GATAA-3' play an important role in DAL5 gene expression, probably representing a portion of the binding site for a transcriptional activation factor.

This article has been cited by other articles:

• Cai, H., Hauser, M., Naider, F., Becker, J. M. (2007). Differential Regulation and Substrate Preferences in Two Peptide Transporters of Saccharomyces cerevisiae. Eukaryot Cell 6: 1805-1813 [Abstract] [Full Text]  
• Ligr, M., Siddharthan, R., Cross, F. R., Siggia, E. D. (2006). Gene Expression From Random Libraries of Yeast Promoters. Genetics 172: 2113-2122 [Abstract] [Full Text]  
• Schwimmer, C., Masison, D. C. (2002). Antagonistic Interactions between Yeast [PSI+] and [URE3] Prions and Curing of [URE3] by Hsp70 Protein Chaperone Ssa1p but Not by Ssa2p. Mol. Cell. Biol. 22: 3590-3598 [Abstract] [Full Text]  
• Tate, J. J., Cox, K. H., Rai, R., Cooper, T. G. (2002). Mks1p Is Required for Negative Regulation of Retrograde Gene Expression in Saccharomyces cerevisiae but Does Not Affect Nitrogen Catabolite Repression-sensitive Gene Expression. J. Biol. Chem. 277: 20477-20482 [Abstract] [Full Text]  
• Distler, M., Kulkarni, A., Rai, R., Cooper, T. G. (2001). Green Fluorescent Protein-Dal80p Illuminates up to 16 Distinct Foci That Colocalize with and Exhibit the Same Behavior as Chromosomal DNA Proceeding through the Cell Cycle of Saccharomyces cerevisiae. J. Bacteriol. 183: 4636-4642 [Abstract] [Full Text]  
• Scott, S., Dorrington, R., Svetlov, V., Beeser, A. E., Distler, M., Cooper, T. G. (2000). Functional Domain Mapping and Subcellular Distribution of Dal82p in Saccharomyces cerevisiae. J. Biol. Chem. 275: 7198-7204 [Abstract] [Full Text]  
• Edskes, H. K., Hanover, J. A., Wickner, R. B. (1999). Mks1p Is a Regulator of Nitrogen Catabolism Upstream of Ure2p in Saccharomyces cerevisiae. Genetics 153: 585-594 [Abstract] [Full Text]  
• Rai, R., Daugherty, J. R., Cunningham, T. S., Cooper, T. G. (1999). Overlapping Positive and Negative GATA Factor Binding Sites Mediate Inducible DAL7 Gene Expression in Saccharomyces cerevisiae. J. Biol. Chem. 274: 28026-28034 [Abstract] [Full Text]  
• Iraqui, I., Vissers, S., Andre, B., Urrestarazu, A. (1999). Transcriptional Induction by Aromatic Amino Acids in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 3360-3371 [Abstract] [Full Text]  
• Svetlov, V. V., Cooper, T. G. (1998). The Saccharomyces cerevisiae GATA Factors Dal80p and Deh1p Can Form Homo- and Heterodimeric Complexes. J. Bacteriol. 180: 5682-5688 [Abstract] [Full Text]  
• Haas, H., Angermayr, K., Zadra, I., Stoffler, G. (1997). Overexpression of nreB, a New GATA Factor-encoding Gene of Penicillium chrysogenum, Leads to Repression of the Nitrate Assimilatory Gene Cluster. J. Biol. Chem. 272: 22576-22582 [Abstract] [Full Text]  
• Kulkarni, A. A., Abul-Hamd, A. T., Rai, R., El Berry, H., Cooper, T. G. (2001). Gln3p Nuclear Localization and Interaction with Ure2p in Saccharomyces cerevisiae. J. Biol. Chem. 276: 32136-32144 [Abstract] [Full Text]  
• Pierce, M. M., Maddelein, M.-L., Roberts, B. T., Wickner, R. B. (2001). A novel Rtg2p activity regulates nitrogen catabolism in yeast. Proc. Natl. Acad. Sci. USA 98: 13213-13218 [Abstract] [Full Text]