This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ryden, T A
Right arrow Articles by Beemon, K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryden, T A
Right arrow Articles by Beemon, K

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 March; 9(3): 1155-1164

Avian retroviral long terminal repeats bind CCAAT/enhancer-binding protein.

T A Ryden and K Beemon

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.

ABSTRACT

DNA-protein interactions involving enhancer and promoter sequences within the U3 regions of several avian retroviral long terminal repeats (LTRs) were studied by DNase I footprinting. The rat CCAAT/enhancer-binding protein, C/EBP, bound to all four viral LTRs examined. The Rous sarcoma virus binding site corresponded closely to the 5' limit of the LTR enhancer; nucleotides -225 to -188 were protected as a pair of adjacent binding domains. The Fujinami sarcoma virus LTR bound C/EBP at a single site at nucleotides -213 to -195. C/EBP also bound to the promoter region of the enhancerless Rous-associated virus-0 LTR at nucleotides -77 to -57. The avian myeloblastosis virus LTR bound C/EBP at three sites: nucleotides -262 to -246, -154 to -134, and -55 to -39. We have previously observed binding of C/EBP to an enhancer in the gag gene of avian retroviruses. A heat-treated nuclear extract from chicken liver bound to all of the same retroviral sequences as did C/EBP. Alignment of the avian retroviral binding sequences with the published binding sites for C/EBP in two CCAAT boxes and in the simian virus 40, polyoma, and murine sarcoma virus enhancers suggested TTGNNGCTAATG as a consensus sequence for binding of C/EBP. When two bases of this consensus sequence were altered by site-specific mutagenesis of the Rous sarcoma virus LTR, binding of the heat-stable chicken protein was eliminated.

Mol Cell Biol. 1989 March; 9(3): 1155-1164




This article has been cited by other articles:

  • Oh, W. J., Rishi, V., Orosz, A., Gerdes, M. J., Vinson, C. (2007). Inhibition of CCAAT/Enhancer Binding Protein Family DNA Binding in Mouse Epidermis Prevents and Regresses Papillomas. Cancer Res. 67: 1867-1876 [Abstract] [Full Text]  
  • Courselaud, B., Pigeon, C., Inoue, Y., Inoue, J., Gonzalez, F. J., Leroyer, P., Gilot, D., Boudjema, K., Guguen-Guillouzo, C., Brissot, P., Loreal, O., Ilyin, G. (2002). C/EBPalpha Regulates Hepatic Transcription of Hepcidin, an Antimicrobial Peptide and Regulator of Iron Metabolism. CROSS-TALK BETWEEN C/EBP PATHWAY AND IRON METABOLISM. J. Biol. Chem. 277: 41163-41170 [Abstract] [Full Text]  
  • Ishida, H., Ueda, K., Ohkawa, K., Kanazawa, Y., Hosui, A., Nakanishi, F., Mita, E., Kasahara, A., Sasaki, Y., Hori, M., Hayashi, N. (2000). Identification of Multiple Transcription Factors, HLF, FTF, and E4BP4, Controlling Hepatitis B Virus Enhancer II. J. Virol. 74: 1241-1251 [Abstract] [Full Text]  
  • Belsham, D. D., Mellon, P. L. (2000). Transcription Factors Oct-1 and C/EBP{beta} (CCAAT/Enhancer-Binding Protein-{beta}) Are Involved in the Glutamate/Nitric Oxide/cyclic-Guanosine 5'-Monophosphate-Mediated Repression of Gonadotropin-Releasing Hormone Gene Expression. Mol. Endocrinol. 14: 212-228 [Abstract] [Full Text]  
  • Krasko, A., Schroder, H. C., Perovic, S., Steffen, R., Kruse, M., Reichert, W., Muller, I. M., Muller, W. E. G. (1999). Ethylene Modulates Gene Expression in Cells of the Marine Sponge Suberites domuncula and Reduces the Degree of Apoptosis. J. Biol. Chem. 274: 31524-31530 [Abstract] [Full Text]  
  • Yamada, K., Duong, D. T., Scott, D. K., Wang, J.-C., Granner, D. K. (1999). CCAAT/Enhancer-binding Protein beta  Is an Accessory Factor for the Glucocorticoid Response from the cAMP Response Element in the Rat Phosphoenolpyruvate Carboxykinase Gene Promoter. J. Biol. Chem. 274: 5880-5887 [Abstract] [Full Text]  
  • Mietus-Snyder, M., Glass, C. K., Pitas, R. E. (1998). Transcriptional Activation of Scavenger Receptor Expression in Human Smooth Muscle Cells Requires AP-1/c-Jun and C/EBPß : Both AP-1 Binding and JNK Activation Are Induced by Phorbol Esters and Oxidative Stress. Arterioscler. Thromb. Vasc. Bio. 18: 1440-1449 [Abstract] [Full Text]  
  • Vorgia, P., Zannis, V. I., Kardassis, D. (1998). A Short Proximal Promoter and the Distal Hepatic Control Region-1 (HCR-1) Contribute to the Liver Specificity of the Human Apolipoprotein C-II Gene. HEPATIC ENHANCEMENT BY HCR-1 REQUIRES TWO PROXIMAL HORMONE RESPONSE ELEMENTS WHICH HAVE DIFFERENT BINDING SPECIFICITIES FOR ORPHAN RECEPTORS HNF-4, ARP-1, and EAR-2. J. Biol. Chem. 273: 4188-4196 [Abstract] [Full Text]  
  • Rao, N. V., Rao, G. V., Hoidal, J. R. (1997). Human Dipeptidyl-peptidase I. GENE CHARACTERIZATION, LOCALIZATION, AND EXPRESSION. J. Biol. Chem. 272: 10260-10265 [Abstract] [Full Text]  
  • Yano, S., Fukunaga, K., Takiguchi, M., Ushio, Y., Mori, M., Miyamoto, E. (1996). Regulation of CCAAT/Enhancer-binding Protein Family Members by Stimulation of Glutamate Receptors in Cultured Rat Cortical Astrocytes. J. Biol. Chem. 271: 23520-23527 [Abstract] [Full Text]  
  • Thomas, M. J., Umayahara, Y., Shu, H., Centrella, M., Rotwein, P., McCarthy, T. L. (1996). Identification of the cAMP Response Element That Controls Transcriptional Activation of the Insulin-like Growth Factor-I Gene by Prostaglandin E2 in Osteoblasts. J. Biol. Chem. 271: 21835-21841 [Abstract] [Full Text]  
  • Osada, S., Yamamoto, H., Nishihara, T., Imagawa, M. (1996). DNA Binding Specificity of the CCAAT/Enhancer-binding Protein Transcription Factor Family. J. Biol. Chem. 271: 3891-3896 [Abstract] [Full Text]  
  • Figueiredo, M. S., Brownlee, G. G. (1995). cis-Acting Elements and Transcription Factors Involved in the Promoter Activity of the Human Factor VIII Gene. J. Biol. Chem. 270: 11828-11838 [Abstract] [Full Text]  
  • Hann, S R, Dixit, M, Sears, R C, Sealy, L (1994). The alternatively initiated c-Myc proteins differentially regulate transcription through a noncanonical DNA-binding site.. Genes Dev. 8: 2441-2452 [Abstract]  
  • Angrand, P. O., Rousset, J. P., Weiss, M. C. (1992). Cell phenotype, binding affinity and promoter structure modulate transactivation by HNF1 and LAP. J. Cell Sci. 103: 1083-1092 [Abstract]  
  • Wegner, M., Cao, Z., Rosenfeld, M. G. (1992). Calcium-Regulated Phosphorylation Within the Leucine Zipper of C/EBPbeta. Science 256: 370-373 [Abstract]  
  • Roman, C, Platero, J S, Shuman, J, Calame, K (1990). Ig/EBP-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to C/EBP and heterodimerizes with C/EBP.. Genes Dev. 4: 1404-1415 [Abstract]  
  • Dutta, A, Stoeckle, M Y, Hanafusa, H (1990). Serum and v-src increase the level of a CCAAT-binding factor required for transcription from a retroviral long terminal repeat.. Genes Dev. 4: 243-254 [Abstract]  
  • Christy, R J, Yang, V W, Ntambi, J M, Geiman, D E, Landschulz, W H, Friedman, A D, Nakabeppu, Y, Kelly, T J, Lane, M D (1989). Differentiation-induced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte-specific genes.. Genes Dev. 3: 1323-1335 [Abstract]  
  • Yeagley, D., Moll, J., Vinson, C. A., Quinn, P. G. (2000). Characterization of Elements Mediating Regulation of Phosphoenolpyruvate Carboxykinase Gene Transcription by Protein Kinase A and Insulin. IDENTIFICATION OF A DISTINCT COMPLEX FORMED IN CELLS THAT MEDIATE INSULIN INHIBITION. J. Biol. Chem. 275: 17814-17820 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»