This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mestel, R Articles by Holland, M J Search for Related Content PubMed PubMed Citation Articles by Mestel, R Articles by Holland, M J

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 March; 9(3): 1243-1254

Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities.

Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.

ABSTRACT

Sequences within the spacer region of yeast rRNA cistrons stimulate synthesis of the major 35S rRNA precursor in vivo 10- to 30-fold (E. A. Elion and J. R. Warner, Cell 39:663-673, 1984). Spacer sequences that mediate this stimulatory activity are located approximately 2.2 kilobases upstream from sequences that encode the 5' terminus of the 35S rRNA precursor. By utilizing a centromere-containing plasmid carrying a 35S rRNA minigene, a 160-base-pair region of spacer rDNA was identified by deletion mapping that is required for efficient stimulation of 35S rRNA synthesis in vivo. A 22-base-pair sequence, previously shown to support RNA polymerase I-dependent selective initiation of transcription in vitro, was located 15 base pairs upstream from the 3' boundary of the stimulatory region. A 77-base pair region of spacer DNA that mediates transcriptional terminator activity in vivo was identified immediately downstream from the 5' boundary of the stimulatory region. Deletion mutations extending downstream from the 5' boundary of the 160-base-pair stimulatory region simultaneously interfere with terminator activity and stimulation of 35S rRNA synthesis from the minigene. The terminator region supported termination of transcripts initiated by RNA polymerase I in vivo. The organization of sequences that support terminator and promoter activities within the 160-base-pair stimulatory region is similar to the organization of rDNA gene promoters in higher organisms. Possible mechanisms for spacer-sequence-dependent stimulation of yeast 35S rRNA synthesis in vivo are discussed.

This article has been cited by other articles:

• Kang, J. J., Yokoi, T. J., Holland, M. J. (1995). Binding Sites for Abundant Nuclear Factors Modulate RNA Polymerase I-dependent Enhancer Function in Saccharomyces cerevisiae. J. Biol. Chem. 270: 28723-28732 [Abstract] [Full Text]  
• Chasman, D I, Lue, N F, Buchman, A R, LaPointe, J W, Lorch, Y, Kornberg, R D (1990). A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.. Genes Dev. 4: 503-514 [Abstract]