This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yutzey, K E
Right arrow Articles by Konieczny, S F
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yutzey, K E
Right arrow Articles by Konieczny, S F

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 April; 9(4): 1397-1405

An internal regulatory element controls troponin I gene expression.

K E Yutzey, R L Kline and S F Konieczny

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.

ABSTRACT

During skeletal myogenesis, approximately 20 contractile proteins and related gene products temporally accumulate as the cells fuse to form multinucleated muscle fibers. In most instances, the contractile protein genes are regulated transcriptionally, which suggests that a common molecular mechanism may coordinate the expression of this diverse and evolutionarily unrelated gene set. Recent studies have examined the muscle-specific cis-acting elements associated with numerous contractile protein genes. All of the identified regulatory elements are positioned in the 5'-flanking regions, usually within 1,500 base pairs of the transcription start site. Surprisingly, a DNA consensus sequence that is common to each contractile protein gene has not been identified. In contrast to the results of these earlier studies, we have found that the 5'-flanking region of the quail troponin I (TnI) gene is not sufficient to permit the normal myofiber transcriptional activation of the gene. Instead, the TnI gene utilizes a unique internal regulatory element that is responsible for the correct myofiber-specific expression pattern associated with the TnI gene. This is the first example in which a contractile protein gene has been shown to rely primarily on an internal regulatory element to elicit transcriptional activation during myogenesis. The diversity of regulatory elements associated with the contractile protein genes suggests that the temporal expression of the genes may involve individual cis-trans regulatory components specific for each gene.

Mol Cell Biol. 1989 April; 9(4): 1397-1405




This article has been cited by other articles:

  • Rana, Z. A., Gundersen, K., Buonanno, A. (2008). Activity-dependent repression of muscle genes by NFAT. Proc. Natl. Acad. Sci. USA 105: 5921-5926 [Abstract] [Full Text]  
  • Rana, Z. A, Gundersen, K., Buonanno, A., Vullhorst, D. (2005). Imaging transcription in vivo: distinct regulatory effects of fast and slow activity patterns on promoter elements from vertebrate troponin I isoform genes. J. Physiol. 562: 815-828 [Abstract] [Full Text]  
  • Marin, M.-C., Rodriguez, J.-R., Ferrus, A. (2004). Transcription of Drosophila Troponin I Gene Is Regulated by Two Conserved, Functionally Identical, Synergistic Elements. Mol. Biol. Cell 15: 1185-1196 [Abstract] [Full Text]  
  • Calvo, S., Vullhorst, D., Venepally, P., Cheng, J., Karavanova, I., Buonanno, A. (2001). Molecular Dissection of DNA Sequences and Factors Involved in Slow Muscle-Specific Transcription. Mol. Cell. Biol. 21: 8490-8503 [Abstract] [Full Text]  
  • Calvo, S., Venepally, P., Cheng, J., Buonanno, A. (1999). Fiber-Type-Specific Transcription of the Troponin I Slow Gene Is Regulated by Multiple Elements. Mol. Cell. Biol. 19: 515-525 [Abstract] [Full Text]  
  • Prado, A., Canal, I., Ferrús, A. (1999). The Haplolethal Region at the 16F Gene Cluster of Drosophila melanogaster: Structure and Function. Genetics 151: 163-175 [Abstract] [Full Text]  
  • Storbeck, C. J., Sabourin, L. A., Waring, J. D., Korneluk, R. G. (1998). Definition of Regulatory Sequence Elements in the Promoter Region and the First Intron of the Myotonic Dystrophy Protein Kinase Gene. J. Biol. Chem. 273: 9139-9147 [Abstract] [Full Text]  
  • Gao, J., Li, Z., Paulin, D. (1998). A Novel Site, Mt, in the Human Desmin Enhancer Is Necessary for Maximal Expression in Skeletal Muscle. J. Biol. Chem. 273: 6402-6409 [Abstract] [Full Text]  
  • Criswell, D. S., Hodgson, V. R. M., Hardeman, E. C., Booth, F. W. (1998). Nerve-responsive troponin I slow promoter does not respond to unloading. J. Appl. Physiol. 84: 1083-1087 [Abstract] [Full Text]  
  • Sasner, M., Buonanno, A. (1996). Distinct N-Methyl--aspartate Receptor 2B Subunit Gene Sequences Confer Neural and Developmental Specific Expression. J. Biol. Chem. 271: 21316-21322 [Abstract] [Full Text]  
  • Tae, H.-J., Zhang, S., Kim, K.-H. (1995). cAMP Activation of CAAT Enhancer-binding Protein-beta Gene Expression and Promoter I of Acetyl-CoA Carboxylase. J. Biol. Chem. 270: 21487-21494 [Abstract] [Full Text]  
  • Hallauer, P. L., Bradshaw, H. L., Hastings, K. E. (1993). Complex fiber-type-specific expression of fast skeletal muscle troponin I gene constructs in transgenic mice. Development 119: 691-701 [Abstract]  
  • Li, Z, Marchand, P, Humbert, J, Babinet, C, Paulin, D (1993). Desmin sequence elements regulating skeletal muscle-specific expression in transgenic mice. Development 117: 947-959 [Abstract]  
  • Brennan, T J, Olson, E N (1990). Myogenin resides in the nucleus and acquires high affinity for a conserved enhancer element on heterodimerization.. Genes Dev. 4: 582-595 [Abstract]  
  • Rhodes, S J, Konieczny, S F (1989). Identification of MRF4: a new member of the muscle regulatory factor gene family.. Genes Dev. 3: 2050-2061 [Abstract]  
  • Charbonnier, F., Gaspera, B. D., Armand, A.-S., Van der Laarse, W. J., Launay, T., Becker, C., Gallien, C.-L., Chanoine, C. (2002). Two Myogenin-related Genes Are Differentially Expressed in Xenopus laevis Myogenesis and Differ in Their Ability to Transactivate Muscle Structural Genes. J. Biol. Chem. 277: 1139-1147 [Abstract] [Full Text]  
  • Hallauer, P. L., Hastings, K. E. M. (2002). Coregulation of fast contractile protein transgene and glycolytic enzyme expression in mouse skeletal muscle. Am. J. Physiol. Cell Physiol. 282: C113-C124 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»