This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Price, D H Articles by Greenleaf, A L Search for Related Content PubMed PubMed Citation Articles by Price, D H Articles by Greenleaf, A L

Dynamic interaction between a Drosophila transcription factor and RNA polymerase II.

Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.

ABSTRACT

We have purified factor 5, a Drosophila RNA polymerase II transcription factor. Factor 5 was found to be required for accurate initiation of transcription from specific promoters and also had a dramatic effect on the elongation properties of RNA polymerase II. Kinetic studies suggested that factor 5 stimulates the elongation rate of RNA polymerase II on a dC-tailed, double-stranded template by reducing the time spent at the numerous pause sites encountered by the polymerase. The factor was found to be composed of two polypeptides (34 and 86 kilodaltons). Both subunits bound tightly to pure RNA polymerase II but were not bound to polymerase in elongation complexes. Our results suggest that factor 5 interacts briefly with the paused polymerase molecules and catalyzes a conformational change in them such that they adopt an elongation-competent conformation.

This article has been cited by other articles:

• Cheng, B., Price, D. H. (2007). Properties of RNA Polymerase II Elongation Complexes Before and After the P-TEFb-mediated Transition into Productive Elongation. J. Biol. Chem. 282: 21901-21912 [Abstract] [Full Text]  
• Gerber, M., Eissenberg, J. C., Kong, S., Tenney, K., Conaway, J. W., Conaway, R. C., Shilatifard, A. (2004). In Vivo Requirement of the RNA Polymerase II Elongation Factor Elongin A for Proper Gene Expression and Development. Mol. Cell. Biol. 24: 9911-9919 [Abstract] [Full Text]  
• Sims, R. J. III, Belotserkovskaya, R., Reinberg, D. (2004). Elongation by RNA polymerase II: the short and long of it. Genes Dev. 18: 2437-2468 [Abstract] [Full Text]  
• Gerber, M., Shilatifard, A. (2003). Transcriptional Elongation by RNA Polymerase II and Histone Methylation. J. Biol. Chem. 278: 26303-26306 [Abstract] [Full Text]  
• Adamson, T. E., Price, D. H. (2003). Cotranscriptional Processing of Drosophila Histone mRNAs. Mol. Cell. Biol. 23: 4046-4055 [Abstract] [Full Text]  
• Nedialkov, Y. A., Gong, X. Q., Hovde, S. L., Yamaguchi, Y., Handa, H., Geiger, J. H., Yan, H., Burton, Z. F. (2003). NTP-driven Translocation by Human RNA Polymerase II. J. Biol. Chem. 278: 18303-18312 [Abstract] [Full Text]  
• Yamazaki, K., Aso, T., Ohnishi, Y., Ohno, M., Tamura, K., Shuin, T., Kitajima, S., Nakabeppu, Y. (2003). Mammalian Elongin A Is Not Essential for Cell Viability but Is Required for Proper Cell Cycle Progression with Limited Alteration of Gene Expression. J. Biol. Chem. 278: 13585-13589 [Abstract] [Full Text]  
• Funk, J. D., Nedialkov, Y. A., Xu, D., Burton, Z. F. (2002). A Key Role for the alpha 1 Helix of Human RAP74 in the Initiation and Elongation of RNA Chains. J. Biol. Chem. 277: 46998-47003 [Abstract] [Full Text]  
• Krogan, N. J., Kim, M., Ahn, S. H., Zhong, G., Kobor, M. S., Cagney, G., Emili, A., Shilatifard, A., Buratowski, S., Greenblatt, J. F. (2002). RNA Polymerase II Elongation Factors of Saccharomyces cerevisiae: a Targeted Proteomics Approach. Mol. Cell. Biol. 22: 6979-6992 [Abstract] [Full Text]  
• Koiwa, H., Barb, A. W., Xiong, L., Li, F., McCully, M. G., Lee, B.-h., Sokolchik, I., Zhu, J., Gong, Z., Reddy, M., Sharkhuu, A., Manabe, Y., Yokoi, S., Zhu, J.-K., Bressan, R. A., Hasegawa, P. M. (2002). C-terminal domain phosphatase-like family members (AtCPLs) differentially regulate Arabidopsis thaliana abiotic stress signaling, growth, and development. Proc. Natl. Acad. Sci. USA 99: 10893-10898 [Abstract] [Full Text]  
• Eissenberg, J. C., Ma, J., Gerber, M. A., Christensen, A., Kennison, J. A., Shilatifard, A. (2002). dELL is an essential RNA polymerase II elongation factor with a general role in development. Proc. Natl. Acad. Sci. USA 99: 9894-9899 [Abstract] [Full Text]  
• Yamazaki, K., Guo, L., Sugahara, K., Zhang, C., Enzan, H., Nakabeppu, Y., Kitajima, S., Aso, T. (2002). Identification and Biochemical Characterization of a Novel Transcription Elongation Factor, Elongin A3. J. Biol. Chem. 277: 26444-26451 [Abstract] [Full Text]  
• Krogan, N. J., Greenblatt, J. F. (2001). Characterization of a Six-Subunit Holo-Elongator Complex Required for the Regulated Expression of a Group of Genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 8203-8212 [Abstract] [Full Text]  
• Renner, D. B., Yamaguchi, Y., Wada, T., Handa, H., Price, D. H. (2001). A Highly Purified RNA Polymerase II Elongation Control System. J. Biol. Chem. 276: 42601-42609 [Abstract] [Full Text]  
• Chavez, S., Garcia-Rubio, M., Prado, F., Aguilera, A. (2001). Hpr1 Is Preferentially Required for Transcription of Either Long or G+C-Rich DNA Sequences in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 7054-7064 [Abstract] [Full Text]  
• Gallardo, M., Aguilera, A. (2001). A New Hyperrecombination Mutation Identifies a Novel Yeast Gene, THP1, Connecting Transcription Elongation With Mitotic Recombination. Genetics 157: 79-89 [Abstract] [Full Text]  
• Kobor, M. S., Simon, L. D., Omichinski, J., Zhong, G., Archambault, J., Greenblatt, J. (2000). A Motif Shared by TFIIF and TFIIB Mediates Their Interaction with the RNA Polymerase II Carboxy-Terminal Domain Phosphatase Fcp1p in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 7438-7449 [Abstract] [Full Text]  
• Lehman, A. L., Dahmus, M. E. (2000). The Sensitivity of RNA Polymerase II in Elongation Complexes to C-terminal Domain Phosphatase. J. Biol. Chem. 275: 14923-14932 [Abstract] [Full Text]  
• Ivanov, D., Kwak, Y. T., Guo, J., Gaynor, R. B. (2000). Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties. Mol. Cell. Biol. 20: 2970-2983 [Abstract] [Full Text]  
• Lei, L., Ren, D., Burton, Z. F. (1999). The RAP74 Subunit of Human Transcription Factor IIF Has Similar Roles in Initiation and Elongation. Mol. Cell. Biol. 19: 8372-8382 [Abstract] [Full Text]  
• Kimura, H., Tao, Y., Roeder, R. G., Cook, P. R. (1999). Quantitation of RNA Polymerase II and Its Transcription Factors in an HeLa Cell: Little Soluble Holoenzyme but Significant Amounts of Polymerases Attached to the Nuclear Substructure. Mol. Cell. Biol. 19: 5383-5392 [Abstract] [Full Text]  
• Dallinger, G., Oberkofler, H., Seelos, C., Patsch, W. (1999). Transcriptional elongation of the rat apolipoprotein A-I gene: identification and mapping of two arrest sites and their signals. J. Lipid Res. 40: 1229-1239 [Abstract] [Full Text]  
• Cho, H., Kim, T.-K., Mancebo, H., Lane, W. S., Flores, O., Reinberg, D. (1999). A protein phosphatase functions to recycle RNA polymerase II. Genes Dev. 13: 1540-1552 [Abstract] [Full Text]  
• Coulombe, B., Burton, Z. F. (1999). DNA Bending and Wrapping around RNA Polymerase: a ""Revolutionary"" Model Describing Transcriptional Mechanisms. Microbiol. Mol. Biol. Rev. 63: 457-478 [Abstract] [Full Text]  
• Kuhlman, T. C., Cho, H., Reinberg, D., Hernandez, N. (1999). The General Transcription Factors IIA, IIB, IIF, and IIE Are Required for RNA Polymerase II Transcription from the Human U1 Small Nuclear RNA Promoter. Mol. Cell. Biol. 19: 2130-2141 [Abstract] [Full Text]  
• Chédin, S., Riva, M., Schultz, P., Sentenac, A., Carles, C. (1998). The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination. Genes Dev. 12: 3857-3871 [Abstract] [Full Text]  
• Shilatifard, A. (1998). Factors regulating the transcriptional elongation activity of RNA polymerase II. FASEB J. 12: 1437-1446 [Abstract] [Full Text]  
• Archambault, J., Pan, G., Dahmus, G. K., Cartier, M., Marshall, N., Zhang, S., Dahmus, M. E., Greenblatt, J. (1998). FCP1, the RAP74-Interacting Subunit of a Human Protein Phosphatase That Dephosphorylates the Carboxyl-terminal Domain of RNA Polymerase IIO. J. Biol. Chem. 273: 27593-27601 [Abstract] [Full Text]  
• Moreland, R. J., Hanas, J. S., Conaway, J. W., Conaway, R. C. (1998). Mechanism of Action of RNA Polymerase II Elongation Factor Elongin. MAXIMAL STIMULATION OF ELONGATION REQUIRES CONVERSION OF THE EARLY ELONGATION COMPLEX TO AN ELONGIN-ACTIVABLE FORM. J. Biol. Chem. 273: 26610-26617 [Abstract] [Full Text]  
• Izban, M. G., Parsons, M. A., Sinden, R. R. (1998). Template End-to-End Transposition by RNA Polymerase II. J. Biol. Chem. 273: 27009-27016 [Abstract] [Full Text]  
• Lennon, J. C. III, Wind, M., Saunders, L., Hock, M. B., Reines, D. (1998). Mutations in RNA Polymerase II and Elongation Factor SII Severely Reduce mRNA Levels in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 5771-5779 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Lei, L., Ren, D., Finkelstein, A., Burton, Z. F. (1998). Functions of the N- and C-Terminal Domains of Human RAP74 in Transcriptional Initiation, Elongation, and Recycling of RNA Polymerase II. Mol. Cell. Biol. 18: 2130-2142 [Abstract] [Full Text]  
• Wada, T., Takagi, T., Yamaguchi, Y., Ferdous, A., Imai, T., Hirose, S., Sugimoto, S., Yano, K., Hartzog, G. A., Winston, F., Buratowski, S., Handa, H. (1998). DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs. Genes Dev. 12: 343-356 [Abstract] [Full Text]  
• Archambault, J., Chambers, R. S., Kobor, M. S., Ho, Y., Cartier, M., Bolotin, D., Andrews, B., Kane, C. M., Greenblatt, J. (1997). An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 94: 14300-14305 [Abstract] [Full Text]  
• Chavez, S., Aguilera, A. (1997). The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability. Genes Dev. 11: 3459-3470 [Abstract] [Full Text]  
• Shimoaraiso, M., Nakanishi, T., Kubo, T., Natori, S. (1997). Identification of the Region in Yeast S-II That Defines Species Specificity in Its Interaction with RNA Polymerase II. J. Biol. Chem. 272: 26550-26554 [Abstract] [Full Text]  
• Pan, G., Aso, T., Greenblatt, J. (1997). Interaction of Elongation Factors TFIIS and Elongin A with a Human RNA Polymerase II Holoenzyme Capable of Promoter-specific Initiation and Responsive to Transcriptional Activators. J. Biol. Chem. 272: 24563-24571 [Abstract] [Full Text]  
• Shilatifard, A., Haque, D., Conaway, R. C., Conaway, J. W. (1997). Structure and Function of RNA Polymerase II Elongation Factor ELL. IDENTIFICATION OF TWO OVERLAPPING ELL FUNCTIONAL DOMAINS THAT GOVERN ITS INTERACTION WITH POLYMERASE AND THE TERNARY ELONGATION COMPLEX. J. Biol. Chem. 272: 22355-22363 [Abstract] [Full Text]  
• Forget, D., Robert, F., Grondin, G., Burton, Z. F., Greenblatt, J., Coulombe, B. (1997). RAP74 induces promoter contacts by RNA polymerase II upstream and downstream of a DNA bend centered on the TATA box. Proc. Natl. Acad. Sci. USA 94: 7150-7155 [Abstract] [Full Text]  
• Cho, H., Maldonado, E., Reinberg, D. (1997). Affinity Purification of a Human RNA Polymerase II Complex Using Monoclonal Antibodies against Transcription Factor IIF. J. Biol. Chem. 272: 11495-11502 [Abstract] [Full Text]  
• Shilatifard, A., Duan, D. R., Haque, D., Florence, C., Schubach, W. H., Conaway, J. W., Conaway, R. C. (1997). ELL2, a new member of an ELL family of RNA polymerase II elongation factors. Proc. Natl. Acad. Sci. USA 94: 3639-3643 [Abstract] [Full Text]  
• Orphanides, G, Lagrange, T, Reinberg, D (1996). The general transcription factors of RNA polymerase II.. Genes Dev. 10: 2657-2683  
• Takagi, Y., Conaway, R. C., Conaway, J. W. (1996). Characterization of Elongin C Functional Domains Required for Interaction with Elongin B and Activation of Elongin A. J. Biol. Chem. 271: 25562-25568 [Abstract] [Full Text]  
• Powell, W., Reines, D. (1996). Mutations in the Second Largest Subunit of RNA Polymerase II Cause 6-Azauracil Sensitivity in Yeast and Increased Transcriptional Arrest in Vitro. J. Biol. Chem. 271: 6866-6873 [Abstract] [Full Text]  
• Chen, Y., Chafin, D., Price, D. H., Greenleaf, A. L. (1996). Drosophila RNA Polymerase II Mutants That Affect Transcription Elongation. J. Biol. Chem. 271: 5993-5999 [Abstract] [Full Text]  
• Yankulov, K., Yamashita, K., Roy, R., Egly, J.-M., Bentley, D. L. (1995). The Transcriptional Elongation Inhibitor 5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole Inhibits Transcription Factor IIH-associated Protein Kinase. J. Biol. Chem. 270: 23922-23925 [Abstract] [Full Text]  
• Takagi, Y., Conaway, J. W., Conaway, R. C. (1995). A Novel Activity Associated with RNA Polymerase II Elongation Factor SIII. J. Biol. Chem. 270: 24300-24305 [Abstract] [Full Text]  
• Aso, T, Lane, W., Conaway, J., Conaway, R. (1995). Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II. Science 269: 1439-1443 [Abstract]  
• Chambers, R. S., Wang, B. Q., Burton, Z. F., Dahmus, M. E. (1995). The Activity of COOH-terminal Domain Phosphatase Is Regulated by a Docking Site on RNA Polymerase II and by the General Transcription Factors IIF and IIB. J. Biol. Chem. 270: 14962-14969 [Abstract] [Full Text]  
• Zawel, L, Kumar, K P, Reinberg, D (1995). Recycling of the general transcription factors during RNA polymerase II transcription.. Genes Dev. 9: 1479-1490 [Abstract]  
• Frank, D. J., Tyree, C. M., George, C. P., Kadonaga, J. T. (1995). Structure and Function of the Small Subunit of TFIIF (RAP30) from Drosophilamelanogaster. J. Biol. Chem. 270: 6292-6297 [Abstract] [Full Text]  
• Izban, M G, Luse, D S (1992). The RNA polymerase II ternary complex cleaves the nascent transcript in a 3'----5' direction in the presence of elongation factor SII.. Genes Dev. 6: 1342-1356 [Abstract]  
• Kato, H, Sumimoto, H, Pognonec, P, Chen, C H, Rosen, C A, Roeder, R G (1992). HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors.. Genes Dev. 6: 655-666 [Abstract]  
• Taira, Y., Kubo, T., Natori, S. (2000). Participation of Transcription Elongation Factor XSII-K1 in Mesoderm-derived Tissue Development in Xenopus laevis. J. Biol. Chem. 275: 32011-32015 [Abstract] [Full Text]  
• Miller, T., Williams, K., Johnstone, R. W., Shilatifard, A. (2000). Identification, Cloning, Expression, and Biochemical Characterization of the Testis-specific RNA Polymerase II Elongation Factor ELL3. J. Biol. Chem. 275: 32052-32056 [Abstract] [Full Text]  
• Elmendorf, B. J., Shilatifard, A., Yan, Q., Conaway, J. W., Conaway, R. C. (2001). Transcription Factors TFIIF, ELL, and Elongin Negatively Regulate SII-induced Nascent Transcript Cleavage by Non-arrested RNA Polymerase II Elongation Intermediates. J. Biol. Chem. 276: 23109-23114 [Abstract] [Full Text]  
• Shimoaraiso, M., Nakanishi, T., Kubo, T., Natori, S. (2000). Transcription Elongation Factor S-II Confers Yeast Resistance to 6-Azauracil by Enhancing Expression of the SSM1 Gene. J. Biol. Chem. 275: 29623-29627 [Abstract] [Full Text]