This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Karin, N J Articles by Fambrough, D M Search for Related Content PubMed PubMed Citation Articles by Karin, N J Articles by Fambrough, D M

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 May; 9(5): 1978-1986

Expression of avian Ca2+-ATPase in cultured mouse myogenic cells.

Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218.

ABSTRACT

cDNA encoding Ca2+-ATPase was cloned from a chicken skeletal muscle library. The cDNA (termed FCa) comprised 3,239 base pairs, including an open reading frame encoding 994 amino acids which showed the highest degree of homology with the adult rabbit fast-twitch Ca2+-ATPase isoform (C. J. Brandl, S. de Leon, D. R. Martin, and D. H. MacLennan, J. Biol. Chem. 262:3768-3774, 1987). Radiolabeled FCa hybridized to a 3.2-kilobase transcript in chicken skeletal muscle RNA but not to cardiac muscle RNA, which confirmed its identity as encoding the fast Ca2+-ATPase isoenzyme. FCa was transfected into the mouse myogenic line C2C12, from which a protein of 100 kilodaltons was immunopurified by using a monoclonal antibody specific for the avian fast Ca2+-ATPase. Immunofluorescence microscopy of a line (designated C2FCa2) stably expressing the avian Ca2+-ATPase localized the protein to the nuclear envelope and a population of cytoplasmic vesicles. A similar pattern was observed when C2FCa2 cells were stained with DiOC6(3), a cyanine dye that labels endoplasmic reticulum and mitochondria (M. Terasaki, J. Song, J. R. Wong, M. J. Weiss, and L. B. Chen, Cell 38:101-108, 1984). We conclude that the avian Ca2+-ATPase fast isoform is expressed and correctly targeted to the endoplasmic reticulum in mouse C2C12 cells.

This article has been cited by other articles:

• Xu, C., Prasad, A. M., Inesi, G., Toyoshima, C. (2008). Critical Role of Val-304 in Conformational Transitions That Allow Ca2+ Occlusion and Phosphoenzyme Turnover in the Ca2+ Transport ATPase. J. Biol. Chem. 283: 3297-3304 [Abstract] [Full Text]  
• Tadini-Buoninsegni, F., Bartolommei, G., Moncelli, M. R., Guidelli, R., Inesi, G. (2006). Pre-steady State Electrogenic Events of Ca2+/H+ Exchange and Transport by the Ca2+-ATPase. J. Biol. Chem. 281: 37720-37727 [Abstract] [Full Text]  
• Obara, K., Miyashita, N., Xu, C., Toyoshima, I., Sugita, Y., Inesi, G., Toyoshima, C. (2005). Inaugural Article: Structural role of countertransport revealed in Ca2+ pump crystal structure in the absence of Ca2+. Proc. Natl. Acad. Sci. USA 102: 14489-14496 [Abstract] [Full Text]  
• Xu, C., Ma, H., Inesi, G., Al-Shawi, M. K., Toyoshima, C. (2004). Specific Structural Requirements for the Inhibitory Effect of Thapsigargin on the Ca2+ ATPase SERCA. J. Biol. Chem. 279: 17973-17979 [Abstract] [Full Text]  
• Ma, H., Sumbilla, C. M., Farrance, I. K. G., Klein, M. G., Inesi, G. (2004). Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes. Am. J. Physiol. Cell Physiol. 286: C556-C564 [Abstract] [Full Text]  
• Ma, H., Inesi, G., Toyoshima, C. (2003). Substrate-induced Conformational Fit and Headpiece Closure in the Ca2+ATPase (SERCA). J. Biol. Chem. 278: 28938-28943 [Abstract] [Full Text]  
• Chen, D., Zhang, Z., Wheatly, M. G., Gao, Y. (2002). Cloning and characterization of the heart muscle isoform of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) from crayfish. J. Exp. Biol. 205: 2677-2686 [Abstract] [Full Text]  
• O'Donnell, J. M., Sumbilla, C. M., Ma, H., Farrance, I. K. G., Cavagna, M., Klein, M. G., Inesi, G. (2001). Tight Control of Exogenous SERCA Expression Is Required to Obtain Acceleration of Calcium Transients With Minimal Cytotoxic Effects in Cardiac Myocytes. Circ. Res. 88: 415-421 [Abstract] [Full Text]  
• Zhang, Z, Chen, D, Wheatly, M. (2000). Cloning and characterization of sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) from crayfish axial muscle. Sarco/Endoplasmic Reticulum Ca(2+)-ATPase. J. Exp. Biol. 203: 3411-3423 [Abstract]  
• Sumbilla, C., Cavagna, M., Zhong, L., Ma, H., Lewis, D., Farrance, I., Inesi, G. (1999). Comparison of SERCA1 and SERCA2a expressed in COS-1 cells and cardiac myocytes. Am. J. Physiol. Heart Circ. Physiol. 277: H2381-H2391 [Abstract] [Full Text]  
• Strock, C., Cavagna, M., Peiffer, W. E., Sumbilla, C., Lewis, D., Inesi, G. (1998). Direct Demonstration of Ca2+ Binding Defects in Sarco-Endoplasmic Reticulum Ca2+ ATPase Mutants Overexpressed in COS-1 Cells Transfected with Adenovirus Vectors. J. Biol. Chem. 273: 15104-15109 [Abstract] [Full Text]  
• Zhong, L., Inesi, G. (1998). Role of the S3 Stalk Segment in the Thapsigargin Concentration Dependence of Sarco-endoplasmic Reticulum Ca2+ ATPase Inhibition. J. Biol. Chem. 273: 12994-12998 [Abstract] [Full Text]  
• Inesi, G., Lewis, D., Sumbilla, C., Nandi, A., Strock, C., Huff, K. W., Rogers, T. B., Johns, D. C., Kessler, P. D., Ordahl, C. P. (1998). Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes. Am. J. Physiol. Cell Physiol. 274: C645-C653 [Abstract] [Full Text]  
• Yu, M., Zhang, L., Rishi, A. K., Khadeer, M., Inesi, G., Hussain, A. (1998). Specific Substitutions at Amino Acid 256 of the Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transport ATPase Mediate Resistance to Thapsigargin in Thapsigargin-resistant Hamster Cells. J. Biol. Chem. 273: 3542-3546 [Abstract] [Full Text]  
• Chen, L., Sumbilla, C., Lewis, D., Zhong, L., Strock, C., Kirtley, M. E., Inesi, G. (1996). Short and Long Range Functions of Amino Acids in the Transmembrane Region of the Sarcoplasmic Reticulum ATPase. J. Biol. Chem. 271: 10745-10752 [Abstract] [Full Text]  
• Kaprielian, Z, Robinson, S., Fambrough, D., Kessler, P. (1996). Movement of Ca(2+)-ATPase molecules within the sarcoplasmic/endoplasmic reticulum in skeletal muscle. J. Cell Sci. 109: 2529-2537 [Abstract]  
• Hussain, A., Garnett, C., Klein, M. G., Tsai-Wu, J.-J., Schneider, M. F., Inesi, G. (1995). Direct Involvement of Intracellular Ca[IMAGE] Transport ATPase in the Development of Thapsigargin Resistance by Chinese Hamster Lung Fibroblasts. J. Biol. Chem. 270: 12140-12146 [Abstract] [Full Text]  
• Camacho, P, Lechleiter, J. (1993). Increased frequency of calcium waves in Xenopus laevis oocytes that express a calcium-ATPase. Science 260: 226-229 [Abstract]  
• Kimura, M, Yamaguchi, Y, Takada, S, Tanabe, K (1993). Cloning of a Ca(2+)-ATPase gene of Plasmodium falciparum and comparison with vertebrate Ca(2+)-ATPases. J. Cell Sci. 104: 1129-1136 [Abstract]  
• Zhang, Z., Lewis, D., Sumbilla, C., Inesi, G., Toyoshima, C. (2001). The Role of the M6-M7 Loop (L67) in Stabilization of the Phosphorylation and Ca2+ Binding Domains of the Sarcoplasmic Reticulum Ca2+-ATPase (SERCA). J. Biol. Chem. 276: 15232-15239 [Abstract] [Full Text]