This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Park, H O Articles by Craig, E A Search for Related Content PubMed PubMed Citation Articles by Park, H O Articles by Craig, E A

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 May; 9(5): 2025-2033

Positive and negative regulation of basal expression of a yeast HSP70 gene.

Department of Physiological Chemistry, University of Wisconsin-Madison 53706.

ABSTRACT

The SSA1 gene, one of the heat-inducible HSP70 genes in the yeast Saccharomyces cerevisiae, also displays a basal level of expression during logarithmic growth. Multiple sites related to the heat shock element (HSE) consensus sequence are present in the SSA1 promoter region (Slater and Craig, Mol. Cell. Biol. 7:1906-1916, 1987). One of the HSEs, HSE2, is important in the basal expression of SSA1 as well as in heat-inducible expression. A promoter containing a mutant HSE2 showed a fivefold-lower level of basal expression and altered kinetics of expression after heat shock. A series of deletion and point mutations led to identification of an upstream repression sequence (URS) which overlapped HSE2. A promoter containing a mutation in the URS showed an increased level of basal expression. A URS-binding activity was detected in yeast whole-cell extracts by a gel electrophoresis DNA-binding assay. The results reported in this paper indicate that basal expression of the SSA1 promoter is determined by both positive and negative elements and imply that the positively acting yeast heat shock factor HSF is responsible, at least in part, for the basal level of expression of SSA1.

This article has been cited by other articles:

• MILLER, G., MITTLER, R. (2006). Could Heat Shock Transcription Factors Function as Hydrogen Peroxide Sensors in Plants?. ANN BOT (LOND) 98: 279-288 [Abstract] [Full Text]  
• Singh, H., Erkine, A. M., Kremer, S. B., Duttweiler, H. M., Davis, D. A., Iqbal, J., Gross, R. R., Gross, D. S. (2006). A Functional Module of Yeast Mediator That Governs the Dynamic Range of Heat-Shock Gene Expression. Genetics 172: 2169-2184 [Abstract] [Full Text]  
• Venturi, C. B., Erkine, A. M., Gross, D. S. (2000). Cell Cycle-Dependent Binding of Yeast Heat Shock Factor to Nucleosomes. Mol. Cell. Biol. 20: 6435-6448 [Abstract] [Full Text]  
• Elkhaimi, M., Kaadige, M. R., Kamath, D., Jackson, J. C., Biliran Jr, H., Lopes, J. M. (2000). Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes. Nucleic Acids Res 28: 3160-3167 [Abstract] [Full Text]  
• Raitt, D. C., Johnson, A. L., Erkine, A. M., Makino, K., Morgan, B., Gross, D. S., Johnston, L. H. (2000). The Skn7 Response Regulator of Saccharomyces cerevisiae Interacts with Hsf1 In Vivo and Is Required for the Induction of Heat Shock Genes by Oxidative Stress. Mol. Biol. Cell 11: 2335-2347 [Abstract] [Full Text]  
• Strich, R, Surosky, R T, Steber, C, Dubois, E, Messenguy, F, Esposito, R E (1994). UME6 is a key regulator of nitrogen repression and meiotic development.. Genes Dev. 8: 796-810 [Abstract]  
• Park, H O, Craig, E A (1991). Transcriptional regulation of a yeast HSP70 gene by heat shock factor and an upstream repression site-binding factor.. Genes Dev. 5: 1299-1308 [Abstract]