This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barnhart, K M Articles by Sheffery, M Search for Related Content PubMed PubMed Citation Articles by Barnhart, K M Articles by Sheffery, M

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 June; 9(6): 2606-2614

Purification and characterization of an erythroid cell-specific factor that binds the murine alpha- and beta-globin genes.

DeWitt Wallace Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York.

ABSTRACT

An erythroid cell-specific nuclear factor that binds tightly to a sequence motif (5'-GATAAGGA-3') shared by many erythroid cell-specific promoters was purified to homogeneity by DNA sequence affinity chromatography. Visualization of the purified factor, which we term EF-1, showed a simple pattern comprising a polypeptide doublet with Mrs of 18,000 and 19,000. We confirmed that these species account for EF-1-binding activity by eluting the polypeptides from sodium dodecyl sulfate-polyacrylamide gels and renaturing the appropriate binding activity. Using the purified polypeptides, we mapped seven factor-binding sites that are dispersed across the murine alpha- and beta-globin genes. The murine alpha-globin gene is flanked by at least two EF-1-binding sites. One site is centered at nucleotide (nt) -180 (with respect to the alpha-globin cap site). A fivefold-weaker site is located downstream of the alpha-globin poly(A) addition site, at nt +1049. We mapped five EF-1-binding sites near the murine beta-globin gene. The strongest site was centered at nt -210. Four additional sites were centered at nt -266 (adjacent to the binding site of a factor present in both murine erythroleukemia and Raji cells), -75 (overlapping the beta-globin CCAAT box), +543 (within the second intervening sequence), and -111.

This article has been cited by other articles:

• Pallante, K. M., Niu, Z., Zhao, Y., Cohen, A. J., Nah, H.-D., Adams, S. L. (1996). The Chick alpha 2(I) Collagen Gene Contains Two Functional Promoters, and Its Expression in Chondrocytes Is Regulated at Both Transcriptional and Post-transcriptional Levels. J. Biol. Chem. 271: 25233-25239 [Abstract] [Full Text]  
• Gächter, T., Werenskiold, A. K., Klemenz, R. (1996). Transcription of the Interleukin-1 Receptor-related T1 Gene Is Initiated at Different Promoters in Mast Cells and Fibroblasts. J. Biol. Chem. 271: 124-129 [Abstract] [Full Text]