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Mol Cell Biol. 1989 June; 9(6): 2761-2764

Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers.

D Shippen-Lentz and E H Blackburn

Department of Molecular Biology, University of California, Berkeley 94720.

ABSTRACT

A telomere terminal transferase activity was identified in developing macronuclear extracts from Euplotes crassus. The activity was essentially unregulated in vitro: up to 50 tandem repeats of the Euplotes telomeric repeat sequence TTTTGGGG were added onto synthetic telomeric oligonucleotide primers. Both the structure of the telomere substrate and its 3'-terminal sequence were recognized. The activity was destroyed by low concentrations of RNase A.

Mol Cell Biol. 1989 June; 9(6): 2761-2764




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