This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lutz-Freyermuth, C Articles by Lutz-Reyermuth, C Search for Related Content PubMed PubMed Citation Articles by Lutz-Freyermuth, C Articles by Lutz-Reyermuth, C

The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs.

Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.

ABSTRACT

The site of interaction between human U1 RNA and one of its uniquely associated proteins, A, was examined with in vitro binding assays. The A protein bound directly to stem-loop II of U1 RNA in a region which exhibits sequence similarity to U2 RNA. The similarity with U2 RNA was in a region that has been shown to interact with a U2 RNA-associated protein. The A protein-binding site on U1 RNA overlapped a previously described epitope for an RNA-specific human autoantibody (S. L. Deutscher and J. D. Keene, Proc. Natl. Acad. Sci. USA 85:3299-3303, 1988), supporting the hypothesis that the anti-RNA antibody originated as an anti-idiotypic response to A protein-specific autoantibodies.

This article has been cited by other articles:

• Lutz, C S, Alwine, J C (1994). Direct interaction of the U1 snRNP-A protein with the upstream efficiency element of the SV40 late polyadenylation signal.. Genes Dev. 8: 576-586 [Abstract]  
• McPheeters, D S, Fabrizio, P, Abelson, J (1989). In vitro reconstitution of functional yeast U2 snRNPs.. Genes Dev. 3: 2124-2136 [Abstract]  
• Urlaub, H., Hartmuth, K., Kostka, S., Grelle, G., Luhrmann, R. (2000). A General Approach for Identification of RNA-Protein Cross-linking Sites within Native Human Spliceosomal Small Nuclear Ribonucleoproteins (snRNPs). ANALYSIS OF RNA-PROTEIN CONTACTS IN NATIVE U1 AND U4/U6.U5 snRNPs. J. Biol. Chem. 275: 41458-41468 [Abstract] [Full Text]