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Mol Cell Biol. 1989 July; 9(7): 3101-3104

Two DNA repair and recombination genes in Saccharomyces cerevisiae, RAD52 and RAD54, are induced during meiosis.

G M Cole, D Schild and R K Mortimer

Genetics Department, University of California, Berkeley 94720.

ABSTRACT

The DNA repair and recombination genes of Saccharomyces cerevisiae, RAD52 and RAD54, were transcriptionally induced approximately 10- to 15-fold in sporulating MATa/alpha cells. Congenic MATa/a cells, which did not sporulate, did not show similar increases. Assays of beta-galactosidase activity in strains harboring either a RAD52- or RAD54-lacZ gene fusion indicated that this induction occurred at a time concomitant with a commitment to meiotic recombination, as measured by prototroph formation from his1 heteroalleles.

Mol Cell Biol. 1989 July; 9(7): 3101-3104




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