This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Felici, F Articles by Hughes, J M Search for Related Content PubMed PubMed Citation Articles by Felici, F Articles by Hughes, J M

The most abundant small cytoplasmic RNA of Saccharomyces cerevisiae has an important function required for normal cell growth.

European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

ABSTRACT

The most abundant RNA visible between 5.8S and 18S rRNA on an ethidium bromide-stained gel of total Saccharomyces cerevisiae RNA has an apparent size of about 600 nucleotides. By purifying the band and using it as a probe to screen a genomic library, we isolated and sequenced the unique gene for this RNA. The transcribed sequence, determined to be 519 nucleotides long, contains elements typical of RNA polymerase III transcription. The RNA is predominantly cytoplasmic, so we called it small cytoplasmic RNA 1 (scR1). ScR1 is neither 3'-polyadenylated nor 5'-trimethylguanosine capped. We constructed a null mutation of the gene by deleting 252 base pairs from the transcribed region. Haploid strains carrying the scr1-delta lesion grew very slowly, segregated cytoplasmic petites [( rho-]) at high frequency, and showed signs of aberrant cell division. A secondary structure model for scR1 shows some of the conserved features of the signal recognition particle 7SL RNAs.

This article has been cited by other articles:

• Copela, L. A., Fernandez, C. F., Sherrer, R. L., Wolin, S. L. (2008). Competition between the Rex1 exonuclease and the La protein affects both Trf4p-mediated RNA quality control and pre-tRNA maturation. RNA 14: 1214-1227 [Abstract] [Full Text]  
• Ulbricht, R. J., Olivas, W. M. (2008). Puf1p acts in combination with other yeast Puf proteins to control mRNA stability. RNA 14: 246-262 [Abstract] [Full Text]  
• Tringe, S. G., Willis, J., Liberatore, K. L., Ruby, S. W. (2006). The WTM Genes in Budding Yeast Amplify Expression of the Stress-Inducible Gene RNR3. Genetics 174: 1215-1228 [Abstract] [Full Text]  
• Foat, B. C., Houshmandi, S. S., Olivas, W. M., Bussemaker, H. J. (2005). Profiling condition-specific, genome-wide regulation of mRNA stability in yeast. Proc. Natl. Acad. Sci. USA 102: 17675-17680 [Abstract] [Full Text]  
• HOUSHMANDI, S. S., OLIVAS, W. M. (2005). Yeast Puf3 mutants reveal the complexity of Puf-RNA binding and identify a loop required for regulation of mRNA decay. RNA 11: 1655-1666 [Abstract] [Full Text]  
• Kuai, L., Das, B., Sherman, F. (2005). A nuclear degradation pathway controls the abundance of normal mRNAs in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 102: 13962-13967 [Abstract] [Full Text]  
• JACKSON, J. S. JR., HOUSHMANDI, S. S., LOPEZ LEBAN, F., OLIVAS, W. M. (2004). Recruitment of the Puf3 protein to its mRNA target for regulation of mRNA decay in yeast. RNA 10: 1625-1636 [Abstract] [Full Text]  
• VAN NUES, R. W., BROWN, J. D. (2004). Saccharomyces SRP RNA secondary structures: A conserved S-domain and extended Alu-domain. RNA 10: 75-89 [Abstract] [Full Text]  
• Kebaara, B., Nazarenus, T., Taylor, R., Forch, A., Atkin, A. L. (2003). The Upf-dependent decay of wild-type PPR1 mRNA depends on its 5'-UTR and first 92 ORF nucleotides. Nucleic Acids Res 31: 3157-3165 [Abstract] [Full Text]  
• Brengues, M., Pintard, L., Lapeyre, B. (2002). mRNA Decay Is Rapidly Induced after Spore Germination of Saccharomyces cerevisiae. J. Biol. Chem. 277: 40505-40512 [Abstract] [Full Text]  
• Regalia, M., Rosenblad, M. A., Samuelsson, T. (2002). Prediction of signal recognition particle RNA genes. Nucleic Acids Res 30: 3368-3377 [Abstract] [Full Text]  
• Ng, H. H., Robert, F., Young, R. A., Struhl, K. (2002). Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex. Genes Dev. 16: 806-819 [Abstract] [Full Text]  
• Dieci, G., Giuliodori, S., Catellani, M., Percudani, R., Ottonello, S. (2002). Intragenic Promoter Adaptation and Facilitated RNA Polymerase III Recycling in the Transcription of SCR1, the 7SL RNA Gene of Saccharomyces cerevisiae. J. Biol. Chem. 277: 6903-6914 [Abstract] [Full Text]  
• Grosshans, H., Deinert, K., Hurt, E., Simos, G. (2001). Biogenesis of the Signal Recognition Particle (SRP) Involves Import of SRP Proteins into the Nucleolus, Assembly with the SRP-RNA, and Xpo1p-mediated Export. J. Cell Biol. 153: 745-762 [Abstract] [Full Text]  
• He, F., Jacobson, A. (2001). Upf1p, Nmd2p, and Upf3p Regulate the Decapping and Exonucleolytic Degradation of both Nonsense-Containing mRNAs and Wild-Type mRNAs. Mol. Cell. Biol. 21: 1515-1530 [Abstract] [Full Text]  
• Briand, J.-F., Navarro, F., Gadal, O., Thuriaux, P. (2001). Cross Talk between tRNA and rRNA Synthesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 189-195 [Abstract] [Full Text]  
• Kanaji, S., Iwahashi, J., Kida, Y., Sakaguchi, M., Mihara, K. (2000). Characterization of the Signal that Directs Tom20 to the Mitochondrial Outer Membrane. J. Cell Biol. 151: 277-288 [Abstract] [Full Text]  
• Bonnerot, C., Boeck, R., Lapeyre, B. (2000). The Two Proteins Pat1p (Mrt1p) and Spb8p Interact In Vivo, Are Required for mRNA Decay, and Are Functionally Linked to Pab1p. Mol. Cell. Biol. 20: 5939-5946 [Abstract] [Full Text]  
• Cai, T., Reilly, T. R., Cerio, M., Schmitt, M. E. (1999). Mutagenesis of SNM1, Which Encodes a Protein Component of the Yeast RNase MRP, Reveals a Role for This Ribonucleoprotein Endoribonuclease in Plasmid Segregation. Mol. Cell. Biol. 19: 7857-7869 [Abstract] [Full Text]  
• Boeck, R., Lapeyre, B., Brown, C. E., Sachs, A. B. (1998). Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant. Mol. Cell. Biol. 18: 5062-5072 [Abstract] [Full Text]  
• Chamberlain, J. R., Lee, Y., Lane, W. S., Engelke, D. R. (1998). Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP. Genes Dev. 12: 1678-1690 [Abstract] [Full Text]  
• George, R., Beddoe, T., Landl, K., Lithgow, T. (1998). The yeast nascent polypeptide-associated complex initiates protein targeting to mitochondria in vivo. Proc. Natl. Acad. Sci. USA 95: 2296-2301 [Abstract] [Full Text]  
• Howe, K. J., Ares, M. Jr. (1997). Intron self-complementarity enforces exon inclusion in a yeast pre-mRNA. Proc. Natl. Acad. Sci. USA 94: 12467-12472 [Abstract] [Full Text]  
• Boisramé, A., Beckerich, J.-M., Gaillardin, C. (1996). Sls1p, an Endoplasmic Reticulum Component, Is Involved in the Protein Translocation Process in the Yeast Yarrowia lipolytica. J. Biol. Chem. 271: 11668-11675 [Abstract] [Full Text]  
• Wells, S E, Neville, M, Haynes, M, Wang, J, Igel, H, Ares, M (1996). CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145.. Genes Dev. 10: 220-232 [Abstract]  
• Schmitt, M E, Clayton, D A (1994). Characterization of a unique protein component of yeast RNase MRP: an RNA-binding protein with a zinc-cluster domain.. Genes Dev. 8: 2617-2628 [Abstract]  
• Decker, C J, Parker, R (1993). A turnover pathway for both stable and unstable mRNAs in yeast: evidence for a requirement for deadenylation.. Genes Dev. 7: 1632-1643 [Abstract]