This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cottarel, G Articles by Hegemann, J H Search for Related Content PubMed PubMed Citation Articles by Cottarel, G Articles by Hegemann, J H

A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.

Institut fuer Mikrobiologie und Molekularbiologie, Justus Liebig Universitaet, Giessen, Federal Republic of Germany.

ABSTRACT

Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, and CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.

This article has been cited by other articles:

• Hayashi, A., Asakawa, H., Haraguchi, T., Hiraoka, Y. (2006). Reconstruction of the Kinetochore during Meiosis in Fission Yeast Schizosaccharomyces pombe. Mol. Biol. Cell 17: 5173-5184 [Abstract] [Full Text]  
• Kelly, J. M., McRobert, L., Baker, D. A. (2006). Evidence on the chromosomal location of centromeric DNA in Plasmodium falciparum from etoposide-mediated topoisomerase-II cleavage. Proc. Natl. Acad. Sci. USA 103: 6706-6711 [Abstract] [Full Text]  
• Nonomura, K.-I., Nakano, M., Eiguchi, M., Suzuki, T., Kurata, N. (2006). PAIR2 is essential for homologous chromosome synapsis in rice meiosis I. J. Cell Sci. 119: 217-225 [Abstract] [Full Text]  
• Wei, R. R., Sorger, P. K., Harrison, S. C. (2005). Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc. Natl. Acad. Sci. USA 102: 5363-5367 [Abstract] [Full Text]  
• Korber, P., Luckenbach, T., Blaschke, D., Horz, W. (2004). Evidence for Histone Eviction in trans upon Induction of the Yeast PHO5 Promoter. Mol. Cell. Biol. 24: 10965-10974 [Abstract] [Full Text]  
• Wu, J., Yamagata, H., Hayashi-Tsugane, M., Hijishita, S., Fujisawa, M., Shibata, M., Ito, Y., Nakamura, M., Sakaguchi, M., Yoshihara, R., Kobayashi, H., Ito, K., Karasawa, W., Yamamoto, M., Saji, S., Katagiri, S., Kanamori, H., Namiki, N., Katayose, Y., Matsumoto, T., Sasaki, T. (2004). Composition and Structure of the Centromeric Region of Rice Chromosome 8. Plant Cell 16: 967-976 [Abstract] [Full Text]  
• Espelin, C. W., Simons, K. T., Harrison, S. C., Sorger, P. K. (2003). Binding of the Essential Saccharomyces cerevisiae Kinetochore Protein Ndc10p to CDEII. Mol. Biol. Cell 14: 4557-4568 [Abstract] [Full Text]  
• Hall, S. E., Kettler, G., Preuss, D. (2003). Centromere Satellites From Arabidopsis Populations: Maintenance of Conserved and Variable Domains. Genome Res. 13: 195-205 [Abstract] [Full Text]  
• Zhong, C. X., Marshall, J. B., Topp, C., Mroczek, R., Kato, A., Nagaki, K., Birchler, J. A., Jiang, J., Dawe, R. K. (2002). Centromeric Retroelements and Satellites Interact with Maize Kinetochore Protein CENH3. Plant Cell 14: 2825-2836 [Abstract] [Full Text]  
• Espelin, C. W., Kaplan, K. B., Sorger, P. K. (1997). Probing the Architecture of a Simple Kinetochore Using DNA-Protein Crosslinking. J. Cell Biol. 139: 1383-1396 [Abstract] [Full Text]  
• Lee, J.-K., Huberman, J. A., Hurwitz, J. (1997). Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 94: 8427-8432 [Abstract] [Full Text]  
• Halverson, D., Baum, M., Stryker, J., Carbon, J., Clarke, L. (1997). A Centromere DNA-binding Protein from Fission Yeast Affects Chromosome Segregation and Has Homology to Human CENP-B. J. Cell Biol. 136: 487-500 [Abstract] [Full Text]