This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Patton, J R Articles by Pederson, T Search for Related Content PubMed PubMed Citation Articles by Patton, J R Articles by Pederson, T

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 August; 9(8): 3360-3368

U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins.

J R Patton, W Habets, W J van Venrooij and T Pederson

Cell Biology Group, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.

ABSTRACT

The U1 small nuclear ribonucleoprotein particle (U1 snRNP), a cofactor in pre-mRNA splicing, contains three proteins, termed 70K, A, and C, that are not present in the other spliceosome-associated snRNPs. We studied the binding of the A and C proteins to U1 RNA, using a U1 snRNP reconstitution system and an antibody-induced nuclease protection technique. Antibodies that reacted with the A and C proteins induced nuclease protection of the first two stem-loops of U1 RNA in reconstituted U1 snRNP. Detailed analysis of the antibody-induced nuclease protection patterns indicated the existence of relatively long-range protein-protein interactions in the U1 snRNP, with the 5' end of U1 RNA and its associated specific proteins interacting with proteins bound to the Sm domain near the 3' end. UV cross-linking experiments in conjunction with an A-protein-specific antibody demonstrated that the A protein bound directly to the U1 RNA rather than assembling in the U1 snRNP exclusively via protein-protein interactions. This conclusion was supported by additional experiments revealing that the A protein could bind to U1 RNA in the absence of bound 70K and Sm core proteins.

---

Mol Cell Biol. 1989 August; 9(8): 3360-3368

This article has been cited by other articles:

• Jacobson, M., Cao, L., Taneja, K, Singer, R., Wang, Y., Pederson, T (1997). Nuclear domains of the RNA subunit of RNase P. J. Cell Sci. 110: 829-837 [Abstract]  
• Temsamani, J., Pederson, T. (1996). The C-group Heterogeneous Nuclear Ribonucleoprotein Proteins Bind to the 5' Stem-Loop of the U2 Small Nuclear Ribonucleoprotein Particle. J. Biol. Chem. 271: 24922-24926 [Abstract] [Full Text]  
• Hannon, G., Maroney, P., Yu, Y., Hannon, G., Nilsen, T. (1992). Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing. Science 258: 1775-1780 [Abstract]