This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Czarnecka, E Articles by Gurley, W B Search for Related Content PubMed PubMed Citation Articles by Czarnecka, E Articles by Gurley, W B

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 August; 9(8): 3457-3463

Regulatory domains of the Gmhsp17.5-E heat shock promoter of soybean.

Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.

ABSTRACT

Promoter domains required for in vivo transcriptional expression of soybean heat shock gene Gmhsp17.5-E were identified by insertion-deletion mutagenesis with transgenic expression monitored in Agrobacterium tumefaciens-incited tumors of sunflower. Removal of the TATA-distal domain from position -1175 to position -259 had little effect on overall activity. The four regions contributing to promoter activity identified by this study all map within 244 base pairs from the start of transcription. The most distal cis-acting element of major significance was located from -244 to -179 and contains a conserved TATA-dyad motif centered at -220. Sequences from -179 to -40 comprise the TATA-proximal domain and include an AT-rich region and two sites containing heat shock consensus elements (HSEs). Deletion of the HSE centered at -93 (site 2) severely reduced transcriptional activity. Heat-inducible expression was also eliminated by internal deletion of either the TATA motif or the overlapping HSEs at site 1, indicating that each of these regions is also a major determinant of promoter activity.

This article has been cited by other articles:

• Sung, D. Y., Vierling, E., Guy, C. L. (2001). Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family. Plant Physiol. 126: 789-800 [Abstract] [Full Text]  
• Schöffl, F., Prändl, R., Reindl, A. (1998). Regulation of the Heat-Shock Response. Plant Physiol. 117: 1135-1141 [Full Text]  
• Halfon, M. S., Kose, H., Chiba, A., Keshishian, H. (1997). Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock. Proc. Natl. Acad. Sci. USA 94: 6255-6260 [Abstract] [Full Text]