This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morgan, W D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morgan, W D

 Previous Article  |  Next Article 

Mol Cell Biol. 1989 September; 9(9): 4099-4104

Transcription factor Sp1 binds to and activates a human hsp70 gene promoter.

W D Morgan

Department of Biology, McGill University, Montreal, Quebec, Canada.

ABSTRACT

I investigated the binding of purified transcription factor Sp1 from HeLa cells to the human hsp70 promoter by DNase I footprinting. Three binding sites were detected within the upstream promoter region, including one located 46 base pairs upstream of the transcription start, between the TATA box and the proximal CCAAT box element. In vitro transcription demonstrated that the proximal site is capable of responding to Sp1-dependent stimulation. These results suggest that Sp1 might contribute to constitutive expression in vivo and might also be involved in the various regulatory responses that affect this gene.

Mol Cell Biol. 1989 September; 9(9): 4099-4104




This article has been cited by other articles:

  • Mandrekar, P., Catalano, D., Jeliazkova, V., Kodys, K. (2008). Alcohol exposure regulates heat shock transcription factor binding and heat shock proteins 70 and 90 in monocytes and macrophages: implication for TNF-{alpha} regulation. J. Leukoc. Biol. 84: 1335-1345 [Abstract] [Full Text]  
  • Venkataraman, G. M., Suciu, D., Groh, V., Boss, J. M., Spies, T. (2007). Promoter Region Architecture and Transcriptional Regulation of the Genes for the MHC Class I-Related Chain A and B Ligands of NKG2D. J. Immunol. 178: 961-969 [Abstract] [Full Text]  
  • Bonner, J. J., Carlson, T., Fackenthal, D. L., Paddock, D., Storey, K., Lea, K. (2000). Complex Regulation of the Yeast Heat Shock Transcription Factor. Mol. Biol. Cell 11: 1739-1751 [Abstract] [Full Text]  
  • McEwen, D. G., Ornitz, D. M. (1998). Regulation of the Fibroblast Growth Factor Receptor 3 Promoter and Intron I Enhancer by Sp1 Family Transcription Factors. J. Biol. Chem. 273: 5349-5357 [Abstract] [Full Text]  
  • Holmberg, C., Roos, P., Lord, J., Eriksson, J., Sistonen, L (1998). Conventional and novel PKC isoenzymes modify the heat-induced stress response but are not activated by heat shock. J. Cell Sci. 111: 3357-3365 [Abstract]  
  • Holmberg, C. I., Leppa, S., Eriksson, J. E., Sistonen, L. (1997). The Phorbol Ester 12-O-Tetradecanoylphorbol 13-Acetate Enhances the Heat-induced Stress Response. J. Biol. Chem. 272: 6792-6798 [Abstract] [Full Text]  
  • Scarpati, E. M., DiCorleto, P. E. (1996). Identification of a Thrombin Response Element in the Human Platelet-derived Growth Factor B-chain (c-sis) Promoter. J. Biol. Chem. 271: 3025-3032 [Abstract] [Full Text]  
  • Abravaya, K, Phillips, B, Morimoto, R I (1991). Attenuation of the heat shock response in HeLa cells is mediated by the release of bound heat shock transcription factor and is modulated by changes in growth and in heat shock temperatures.. Genes Dev. 5: 2117-2127 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»