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Molecular and Cellular Biology, October 1998, p. 5880-5887, Vol. 18, No. 10
0270-7306/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Coactivator TIF1beta Interacts with Transcription Factor C/EBPbeta and Glucocorticoid Receptor To Induce alpha 1-Acid Glycoprotein Gene Expression

Ching-Jin Chang,1 Ya-Ling Chen,2,3 and Sheng-Chung Lee1,2,3,*

Institute of Biological Chemistry, Academia Sinica,1 and Institute of Molecular Medicine2 and H. L. Tsai Memorial Laboratory,3 College of Medicine, National Taiwan University, Taipei, Taiwan

Received 12 March 1998/Returned for modification 12 June 1998/Accepted 14 July 1998

    ABSTRACT
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The transcription of the alpha 1-acid glycoprotein gene is induced by inflammatory cytokines and glucocorticoids. C/EBPbeta is a major transcription factor involved in the induction of the agp gene by some cytokines. In this report, we have identified a novel transcriptional intermediary factor, TIF1beta , which could enhance the transcription of the agp gene by the glucocorticoid receptor (GR) and C/EBPbeta . TIF1beta belongs to a subgroup of RING (really interesting new gene) finger proteins that contain a RING finger preceding two B box-type fingers and a putative coiled-coil domain (RBCC domain). Immunoprecipitation experiments showed that the interaction between GR and TIF1beta is ligand independent. The overexpression of the TIF1beta gene enhances GR-regulated expression in a ligand- and glucocorticoid-responsive element (GRE)-dependent manner. TIF1beta can also augment C/EBPbeta -mediated activity on wild-type and GRE-mutated agp genes, but this augmentation is diminished when all three C/EBPbeta -binding elements are mutated. Functional and biochemical characterizations indicated that the bZIP domain of C/EBPbeta and the RBCC domain, plant homeodomain finger, and bromodomain of TIF1beta are crucial for the interactions of these proteins. Taken together, these results suggest that TIF1beta serves as a converging mediator of signal transduction pathways of glucocorticoids and some inflammatory cytokines.

    INTRODUCTION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

The acute-phase reaction to inflammatory stimuli is accompanied by an increase in a variety of serum proteins, collectively named acute-phase proteins. The synthesis of these proteins is regulated by glucocorticoids and inflammatory cytokines, such as interleukin 1 (IL-1), IL-6, and tumor necrosis factor alpha (5-7, 62). C/EBPbeta was initially identified as the key transcription factor involved in the regulation of the alpha 1-acid glycoprotein (AGP) gene during the acute-phase response (termed AGP/EBP) (18). C/EBPbeta was also shown to be involved in the regulation of a number of other genes, such as those for IL-6 and albumin (termed NF-IL-6, LAP, IL-6DBP, or CRP2) (2, 14, 20, 49, 61). In addition to C/EBPbeta -binding motifs, a glucocorticoid-responsive element (GRE) also exists between -120 and -107 in the 5'-flanking region of the agp gene (8, 18). Previous reports showed that maximal induction of the agp gene by glucocorticoids also requires another C/EBPbeta -binding element located downstream of GRE (34, 50, 60). The synergistic interaction between cytokines and glucocorticoids has been attributed to protein-protein interactions between C/EBPbeta and the glucocorticoid receptor (GR) (45).

GR belongs to a family of nuclear receptors that function as ligand-dependent transcription factors (9, 48). Transcriptional activation of target genes by nuclear receptors is mediated by two activation regions, AF1, located in the N terminus, and AF2, located in the C terminus of the hormone-binding domain of the receptor. GR-mediated transcription is promoter dependent and cell specific (for a review, see reference 24).

Results from studies of transcriptional interference or squelching between AF1 and AF2 of steroid receptors suggested the existence of coactivators or transcriptional intermediary factors which interact specifically with the AF1 and AF2 domains (3, 39, 55). Recent studies have led to the identification of several proteins that interact with nuclear receptors in a ligand-dependent manner and play essential roles in mediating their transcriptional activities. These proteins include RIP140 (15), TIF1 (36), Trip1/SUG1 (38, 59), SRC-1/p160 (26, 31, 47), TIF2/Grip1 (29, 58), ARA70 (63), and CBP/p300 (16, 27, 31). Several of these factors showed markedly different affinities for various nuclear receptors (56, 59). CBP and p300 are large nuclear proteins and have been demonstrated to interact functionally with a number of sequence-specific transcriptional activators (for a review, see reference 30). Previous data indicated that competition for limiting amounts of CBP may account for many of the inhibitory actions of both GR and the retinoic acid receptor on AP1 activation (31).

Genes for two related TIF1 proteins, TIF1alpha and TIF1beta , have been cloned and shown to be members of the RING (really interesting new gene) finger family (for reviews, see references 12 and 52). The RING finger motif can be defined simply as Cys3-His-Cys4, a new class of the zinc finger. At least 80 members of the RING finger family have been identified. Many members, including the tumor suppressor BRCA-1 (42), the oncogene product Mel18 (32), and the mediator of the tumor necrosis factor receptor, TRAF2 (51), have been implicated as being in control of cell growth, cell differentiation, and development. The functions of these RING fingers remain to be defined, although some reports have suggested that they are the interface for protein-protein interactions (4, 10).

To delineate the mechanisms of transcriptional regulation of the agp gene by C/EBPbeta , we have initiated studies on proteins that interact with C/EBPbeta by purifying them using a number of procedures, including anti-C/EBPbeta antibody immunoaffinity chromatography (40, 41). In this report, we present results on the identification and characterization of the roles of TIF1beta in the activation of the agp gene. These results indicate that the enhancement of GR or C/EBPbeta activity by TIF1beta occurs through direct protein-protein interactions.

    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Plasmids and constructs. The EST clone containing partial human TIF1beta cDNA (from nucleotides 1882 to 2673) was obtained from Research Genetics. An 0.8-kb DNA fragment insert isolated from the plasmid was used as a probe for screening the day-16 mouse embryo cDNA library (Novagen). A cDNA clone with a 2.8-kb insert containing the complete open reading frame of TIF1beta was obtained. Mammalian expression plasmids were constructed by cloning the following TIF1beta fragments into cytomegalovirus (CMV) expression vector pcDNA3 (Invitrogen): the full-length EcoRI-HindIII fragment (pcDNA3-TIF1beta ), and EcoRI-SacI fragment (residues 1 to 563), an EcoRI-PvuII fragment (residues 1 to 372), and a fragment resulting from BamHI deletion (residues 80 to 383 deleted) of the full-length EcoRI-HindIII fragment. An SfiI-HindIII fragment (residues 14 to 834), a BamHI fragment (residues 80 to 383), a BamHI-SacI fragment (residues 383 to 563), and a BamHI-HindIII fragment (residues 383 to 834) were cloned into the pGEX-1 vector (Pharmacia) for the production of glutathione S-transferase (GST) fusion proteins. The full-length EcoRI fragment of C/EBPbeta , an N-terminal NcoI fragment (amino acids 21 to 151 [C/EBPbeta -N), or a C-terminal NcoI-HindIII fragment (amino acids 151 to 296 [C/EBPbeta -C]) was ligated to the pRSET vector (Invitrogen) for recombinant protein production.

Other plasmid constructs, such as pCMV-C/EBPbeta , rat AGP (wild type [WT])-CAT, AGP (C mutant)-CAT, AGP (D mutant)-CAT, AGP (E mutant)-CAT, AGP (CDE mutant)-CAT, and AGP (GRE mutant)-CAT, were as described previously (40, 59). Briefly, the plasmids were obtained by ligation of the wild-type or mutant (see below) rat agp gene promoter sequence from -736 to +1 to the chloramphenicol acetyltransferase (CAT) reporter gene. The C, D, E, and GRE mutants correspond to serial 3-base substitutions at positions -74 to -72 (ACA to GTG), -96 to -94 (CAA to TGG), -106 to -104 (AGA to GAG), and -118 to -116 (ACA to GTG), respectively. pMMTV-CAT is the mouse mammary tumor virus long terminal repeat ligated to the CAT reporter gene. The mammalian cell expression vector (pRSV-hGR) and recombinant baculovirus containing GR were kindly provided by M.-J. Tsai of Baylor College of Medicine. pRSV-CREB and pCMV-PKAc were obtained from Susan Taylor.

Recombinant proteins and antibodies. Human TIF1beta (from nucleotide 1882 to 2673) was cloned into the pRSET vector and expressed in Escherichia coli BL21(DE3)(pLysS). This recombinant protein was purified on a nickel column and used for rabbit immunization. Monoclonal and polyclonal antibodies to C/EBPbeta were as described previously (18). Anti-GR antibody was purchased from Santa Cruz Biotech.

Cell cultures, transient transfection, and CAT assay. BHK, HeLa, and P388D1 cells were cultured in Iscove's modified Dulbecco's medium supplemented with 10% fetal calf serum. DNA transfection was performed by the calcium phosphate precipitation method. BHK cells were grown in 6- or 3.5-cm-diameter petri dishes to 30 to 40% confluence. The amounts of CAT reporter plasmid DNA and expression plasmid DNA used in each experiment are described in the figure legends. pcDNA3 plasmid DNA was used to adjust the total amount of DNA for each transfection to be equal. pCMV/SEAP (Tropix) (0.5 µg) was included in each transfection as an internal control for transfection efficiency. During the 24-h posttransfection period, the cells were placed in fresh medium and, in some experiments, induced with 1 µM dexamethasone (water soluble; Sigma). Cells were harvested 24 h later and extracted with 100 µl of 0.25 M Tris-HCl (pH 7.8). The acetylated forms of chloramphenicol were separated by thin-layer chromatography and quantified with an image analyzer (BAS 1000; Fuji). All transfection experiments were repeated two to four times.

Preparation of whole-cell extracts, immunoprecipitation, and Western blotting. Whole-cell extracts from P388D1 cells were prepared by lysing the cells with buffer containing 25 mM HEPES (pH 7.6), 0.3 M NaCl, 1.5 mM MgCl2, 0.2 mM EDTA, 0.5% Nonidet P-40 (NP-40), and 0.5 mM dithiothreitol (DTT). For immunoprecipitation analysis, 1 mg of whole-cell extracts was precleaned with preimmune serum and protein A-Sepharose in 0.5 ml of immunoprecipitation buffer (25 mM HEPES [pH 7.6], 0.25 M NaCl, 1 mM EDTA, 0.1% NP-40, 0.5 mM DTT, 6% glycerol) at 4°C for 2 h. The precleaned supernatants were incubated with 5 µg of anti-TIF1beta or anti-C/EBPbeta antibody and protein A-Sepharose in the presence or absence of 1 µM dexamethasone at 4°C for 90 min. After extensive washes, the protein complex was dissolved in sodium dodecyl sulfate (SDS) loading buffer and subjected to SDS-polyacrylamide gel electrophoresis (PAGE). The separated polypeptides were blotted onto a Hybond-C membrane (Amersham) and probed with anti-TIF1beta , anti-C/EBPbeta , or anti-GR antibody. The results were detected with an enhanced chemiluminescence kit (Amersham).

Protein-protein interaction assay. Glutathione-Sepharose 8A beads (Pharmacia) were mixed with 3 µg of wild-type or deletion mutant recombinant GST-TIF1beta fusion protein or GST only in 500 µl of phosphate-buffered saline containing 1% Triton X-100 on a rotary shaker for 20 min at room temperature. The beads were washed three times with phosphate-buffered saline, combined with 100 ng of recombinant full-length C/EBPbeta , truncated C/EBPbeta -N, or truncated C/EBPbeta -C in a final volume of 500 µl of binding buffer (25 mM HEPES [pH 7.6], 0.25 M NaCl, 1 mM EDTA, 0.1% NP-40, 0.5 mM DTT, 6% glycerol), and incubated on a rotary shaker for 2 h at 4°C. The beads were washed three times with binding buffer, and the bound proteins were subjected to SDS-PAGE and Western blot analysis.

    RESULTS
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

Isolation and characterization of cDNA clones for TIF1beta . The RING finger protein family consists of members found in animals, plants, and viruses, but the function of the RING finger domain remains to be defined. By comparison with sequences of RING finger domains similar to those of inhibitors of apoptosis or RING-1, a number of human EST clones carrying putative RING finger domains were identified. Sequence analysis revealed that one of these clones, containing an 0.8-kb insert, was highly homologous to a mouse protein, TIF1beta (37). Rabbit antibodies were generated by use of a recombinant protein derived from the EST clone. In serendipitous Western blot experiments for identifying C/EBPbeta -interacting proteins, we used a rabbit anti-TIF1beta antibody as a control. Surprisingly, it reacted with a protein of ~100 kDa that appeared in the eluent of the anti-C/EBPbeta immunoaffinity column (data not shown). This observation prompted us to study the possible physical and functional interactions between TIF1beta and C/EBPbeta . Using the 0.8-kb DNA fragment as a probe to screen the mouse cDNA library, a 2.8-kb cDNA clone that could encode a protein of 834 amino acids was obtained.

TIF1beta and TIF1alpha are strongly homologous in the N- and C-terminal regions. The N-terminal region is a RING finger preceding two B box-type fingers and a putative coiled-coil domain (RBCC motif) (36). The C-terminal region consists of a plant homeodomain (PHD) finger followed by a bromodomain (36).

TIF1beta appears to be widely expressed, since Northern blotting reveals a major 3-kb TIF1beta transcript in all human tissues (data not shown). The subcellular localization of TIF1beta was then determined by indirect immunofluorescence, which showed granular staining of TIF1beta only in the nucleoplasm and not in the nucleolus (data not shown).

TIF1beta stimulates the C/EBPbeta -mediated activation of the agp gene. The physical interaction of C/EBPbeta and TIF1beta was examined by an immunoprecipitation assay. P388D1 is a mouse macrophage cell line which expresses C/EBPbeta constitutively. Anti-C/EBPbeta antibody can bring down TIF1beta in P388D1 whole-cell extracts (Fig. 1A). Direct protein-protein interactions were studied by pull-down assays with GST-TIF1beta (full length) and both full-length C/EBPbeta and truncated forms of C/EBPbeta , C/EBPbeta -N and C/EBPbeta -C (Fig. 1B). The results showed that the bZIP domain of C/EBPbeta (i.e., C/EBPbeta -C) is sufficient for its direct interaction with TIF1beta .


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  		FIG. 1.   Protein-protein interactions between C/EBPbeta and TIF1beta . (A) P388D1 whole-cell extracts were immunoprecipitated with anti-C/EBPbeta polyclonal antibody (C/EBPbeta ) or preimmune serum (PI). The precipitated proteins were subjected to SDS-PAGE followed by Western blotting with anti-C/EBPbeta monoclonal or anti-TIF1beta polyclonal antibodies. (B) Several recombinant C/EBPbeta constructs were incubated with glutathione bead-immobilized GST-TIF1beta (lanes 4 to 6) or GST (lanes 7 to 9). After extensive washes, the protein complexes were subjected to SDS-PAGE and immunoblotted with anti-C/EBPbeta antibody. Lanes 1 to 3 represent direct loading of different recombinant C/EBPbeta constructs. FL, N, and C represent full-length C/EBPbeta , C/EBPbeta -N, and C/EBPbeta -C, which are described in Materials and Methods.

To characterize the functional role of TIF1beta in the activation of the agp gene by C/EBPbeta , we performed cotransfection experiments. TIF1beta augmented the activation of the agp gene by C/EBPbeta in a dose-dependent manner (Fig. 2A). To further elucidate the roles of C/EBPbeta -binding motifs in the activation effect between C/EBPbeta and TIF1beta , we performed transfection assays with reporters containing mutated C/EBPbeta -binding motifs (C, D, E, and CDE mutants). Compared to the results obtained with the wild-type reporter gene, there was stimulation of C/EBPbeta activity by TIF1beta with the C-, D-, or E-site-mutated reporter gene (13-fold for the wild type and 26-, 10-, and 24-fold for the C, D, and E mutants, respectively). Induction by these factors was dramatically reduced when a reporter containing mutations of all three C/EBPbeta -binding sites (i.e., the CDE mutant) was tested (Fig. 2B). Transactivation of the GRE-mutated agp gene by C/EBPbeta and TIF1beta was comparable to that of the wild-type gene (Fig. 2B). Thus, the augmentation effect of TIF1beta on the activation of the C/EBPbeta gene is exclusively C/EBPbeta -binding motif dependent, and GR is apparently not required.


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  		FIG. 2.   Stimulation of C/EBPbeta -mediated gene activation by TIF1beta . (A) BHK cells were cotransfected with 2 µg of AGP (WT)-CAT, 0.5 µg of pCMV-C/EBPbeta (C/EBPbeta ), and increasing amounts of pcDNA3-TIF1beta (TIF1beta ) (1, 5, and 10 µg). (B) In 3.5-cm-diameter petri dishes, BHK cells were cotransfected with 0.5 µg of AGP (WT)-CAT, AGP (C mutant)-CAT, AGP (D mutant)-CAT, AGP (E mutant)-CAT, AGP (CDE mutant)-CAT, or AGP (GRE mutant)-CAT and 0.1 µg of pCMV-C/EBPbeta with or without 2 µg of pcDNA3-TIF1beta . The data represent the average activity of two independent duplicate experiments. The fold induction by TIF1beta is indicated below the panel. mt, mutant. (C) (Left panel) In 3.5-cm-diameter petri dishes, BHK cells were cotransfected with 0.5 µg of AGP (WT)-CAT and 0.05 µg of pCMV-C/EBPalpha in the presence or absence of 2 µg of pcDNA3-TIF1beta . (Right panel) BHK cells were transfected with 0.25 µg of pC/EBPbeta -CAT, and 0.1 µg of pCMV-C/EBPbeta or both 0.1 µg of pRSV-CREB and 0.1 µg of pCMV-PKAc in the absence or presence of 2 µg of pcDNA3-TIF1beta . Error bars indicate standard deviations. -, pcDNA3 vector control.

To further assess the activation specificity of TIF1beta and other factors, we conducted transient transfection assays with expression vectors for C/EBPalpha , CREB, and TIF1beta . As shown in Fig. 2C, left panel, TIF1beta also augmented the C/EBPalpha -mediated transactivation of the agp gene. The pC/EBPbeta -CAT reporter contains the promoter from the c-ebpbeta gene (nucleotides -390 to +82) (17). It has been reported that there are C/EBP- and CREB-responsive elements in the regulatory region of c-ebpbeta (17, 44). TIF1beta potentiated C/EBPbeta activity but not CREB activity in the c-ebpbeta gene promoter (Fig. 2C, right panel).

To define the regions of TIF1beta that could interact with C/EBPbeta physically and functionally, we constructed mammalian cell expression vectors and prepared recombinant GST fusion proteins of various deletion mutants of TIF1beta (Fig. 3A and B, upper panels). Mutants of TIF1beta with either the PHD finger and the bromodomain deleted (amino acids 1 to 563) or the RBCC domain deleted (amino acids 80 to 383 deleted) failed to potentiate the activation of the agp gene by C/EBPbeta (Fig. 3A, lower panel). Physical interaction experiments with recombinant proteins derived from C/EBPbeta and deletion mutants of TIF1beta indicated that the RBCC domain of TIF1beta was sufficient to interact with C/EBPbeta (Fig. 3B, lower panel). The region of amino acids 383 to 563 seemed to interact weakly with C/EBPbeta . Taken together, these results suggest that TIF1beta interacts with C/EBPbeta through the RBCC domain and enhances C/EBPbeta transcriptional activity through the PHD finger and the bromodomain.


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  		FIG. 3.   Functional and biochemical characterization of C/EBPbeta -interacting domains of TIF1beta . (A) (Upper panel) Schematic representation of several TIF1beta expression vectors; numbers denote amino acid positions. (Lower panel) Transient transfection assays. BHK cells (in 3.5-cm-diameter petri dishes) were transfected with 0.5 µg of AGP (WT)-CAT, 0.1 µg of pCMV-C/EBPbeta , and 2 µg of pcDNA3-TIF1beta (full length [fl] or from amino acid 1 to 563 or 1 to 372, or full length but with amino acids 80 to 383 deleted). (B) (Upper panel) Schematic representation of several GST-TIF1beta fusion proteins. (Lower panel) Protein pull-down assay. Glutathione bead-immobilized recombinant GST-TIF1beta (fl or from amino acid 80 to 383, 383 to 563, or 383 to 834) incubated with full-length recombinant C/EBPbeta (100 ng). After extensive washes, the protein complex was analyzed by immunoblotting with anti-C/EBPbeta antibody. -, pcDNA3 vector control.

TIF1beta enhances the transcriptional activity of GR. TIF1alpha was identified as a protein that interacts directly with the ligand-binding domains of several nuclear receptors in a ligand- and AF2-dependent manner both in vivo and in vitro. It was suggested that TIF1alpha mediates the transcriptional activation of the target gene by the AF2 domain of nuclear receptors (36). TIF1alpha and TIF1beta share highly conserved domains. To further investigate the role of TIF1beta in nuclear receptor-mediated transactivation of target gene expression, we performed transient transfection assays. As shown in Fig. 4, in the presence of exogenous GR and dexamethasone, TIF1beta stimulated the transcription of mouse mammary tumor virus and the agp promoter in a dose-dependent manner. TIF1beta did not augment the transcriptional activity of GR in the absence of dexamethasone (data not shown).


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  		FIG. 4.   TIF1beta potentiates GR-activated gene expression. (A) HeLa cells were transiently transfected with 2 µg of pMMTV-CAT reporter plasmid, 1 µg of pRSV-hGR (GR), and increasing amounts of pcDNA3-TIF1beta (TIF1beta ) (1, 5, and 10 µg). Each assay was done in the presence of 1 µM dexamethasone. (B) BHK cells were transiently transfected with 2 µg of AGP (WT)-CAT reporter plasmid. Other plasmids and conditions of treatment are as described for panel A. Relative CAT activity normalized with an internal control represents an average of two independent duplicate experiments. Error bars indicate standard deviations.

To determine the molecular basis of target gene activation by TIF1beta and GR, we conducted an analysis of the interaction between TIF1beta and GR by an immunoprecipitation assay. Polyclonal antibody to TIF1beta but not preimmune serum immunoprecipitated GR from P388D1 whole-cell extracts in the absence or presence of dexamethasone (Fig. 5A; the anti-GR antibody detected two isoforms, 95 and 90 kDa). This result indicates that TIF1beta and GR coexist in a complex. Direct protein-protein interactions were examined by an immunoprecipitation assay with GST-TIF1beta fusion protein and recombinant GR. TIF1beta interacted with GR in the absence or presence of dexamethasone (Fig. 5B).


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  		FIG. 5.   Protein-protein interactions between GR and TIF1beta . (A) Immunoprecipitation of GR by anti-TIF1beta antibody. P388D1 whole-cell extracts were immunoprecipitated with anti-TIF1beta or control (PI) antibody in the presence (+) or absence (-) of 1 µM dexamethasone (DEX) and subjected to SDS-PAGE and Western blotting with anti-GR polyclonal antibody. (B) Recombinant GST-TIF1beta was incubated with recombinant GR in the presence (+) or absence (-) of 1 µM dexamethasone and then immunoprecipitated with anti-TIF1beta antibody or preimmune serum. After extensive washes, the protein complex was analyzed by Western blotting with anti-GR antibody. Recombinant GR used for the interaction assay (1/5 input) was included as a control.

Previous studies indicated that maximal induction of the agp gene by glucocorticoid requires the downstream C/EBPbeta -binding sequences (34, 50, 60). To further study the TIF1beta -mediated GR induction of the agp gene, we performed transfection assays using agp promoters containing mutated C/EBPbeta -binding sites or mutated GRE. As indicated by the previous results, the activation of the agp gene by TIF1beta and GR was observed only in the presence of dexamethasone (Fig. 6). Reporters containing mutated C/EBPbeta -binding elements (namely, C, D, and E mutants) remained responsive to TIF1beta and GR. In contrast, the reporter containing mutated GRE was unresponsive to TIF1beta and GR (Fig. 6). The activation of the agp gene by TIF1beta alone in the presence of dexamethasone was likely due to the effect of endogenous GR. These results show that the transcriptional activation of the agp gene by TIF1beta and GR is dependent on GRE but independent of C/EBPbeta -binding motifs.


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  		FIG. 6.   GRE-dependent and C/EBPbeta -binding-element-independent augmentation of the activation of AGP-CAT by GR and TIF1beta . AGP (WT)-CAT, AGP (CDE mutant)-CAT, or AGP (GRE mutant)-CAT was used as a reporter (see Materials and Methods). In 3.5-cm-diameter petri dishes, BHK cells were cotransfected with one reporter plasmid (1 µg) and 0.5 µg of pRSV-hGR (GR), 5 µg of pcDNA3-TIF1beta (TIF1beta ), or both in the presence or absence of 1 µM dexamethasone (DEX). The fold induction for each experiment is shown. The values are the averages of at least two independent experiments. Error bars indicate standard deviations.

We further examined the effect of TIF1beta on the agp gene by cotransfecting pRSV-GR and pCMV-C/EBPbeta simultaneously. As shown in Fig. 7, the net effect of the transactivation of the agp gene by cotransfection of pCMV-C/EBPbeta , pRSV-GR, and pCMV-TIF1beta seemed to be the result of pRSV-GR plus pCMV-TIF1beta . Taken together, these results suggest that there is no additive or synergistic activation of the agp gene by the overexpression of GR, TIF1beta , and C/EBPbeta under these experimental conditions.


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  		FIG. 7.   Activation of the agp gene by C/EBPbeta , GR, and TIF1beta . BHK cells (grown in 6-cm petri dishes) were transfected with 2 µg of AGP (WT)-CAT and 0.5 µg of pCMV-C/EBPbeta (C/EBPbeta ), 1 µg of pRSV-hGR (GR), or 5 µg of pcDNA3-TIF1beta (TIF1beta ) in various combinations in the absence or presence of 1 µM dexamethasone (DEX). Normalized relative CAT activity represents an average of two independent experiments. Error bars indicate standard deviations.

    DISCUSSION
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

TIF1beta was originally identified as a protein that interacts directly with two chromosomal proteins, mHP1alpha and mMOD1 (37). In this report, TIF1beta was identified as a coactivator for C/EBPbeta and GR in the activation of the agp gene. The specificity of TIF1beta for GR or C/EBPbeta was demonstrated by transfection assays with wild-type or mutant reporter plasmids containing GRE and/or C/EBPbeta -binding elements. Direct protein-protein interactions between TIF1beta and GR or C/EBPbeta were apparently responsible for the activation of the agp gene by these proteins. These results suggest that TIF1beta may act as an integrator or coactivator for both glucocorticoid and cytokine signaling pathways leading to the activation of C/EBPbeta . The identification of TIF1beta as a coactivator provides further clues about the mechanisms of transactivation by GR and C/EBPbeta and the regulation of their target genes, such as acute-phase response genes.

The competition for a common coactivator, CBP, by AP-1 and the nuclear receptor provides an example of how genes that contain either an AP-1- or a nuclear receptor-binding site could be regulated (31). However, a more complex pattern of regulation was observed for genes containing composite response elements. One GRE and three C/EBPbeta -binding sites are located in the upstream regulatory region of the agp gene. The regulation of this gene by interactions between TIF1beta , GR, and C/EBPbeta seems to be complex and depends on the steady-state levels of these factors. When pRSV-GR, pCMV-C/EBPbeta , and pCMV-TIF1beta were cotransfected into cells, no apparent changes in the transactivation of the agp gene were seen compared to those seen with pRSV-GR and pCMV-TIF1beta (Fig. 7). In fact, the slight decrease in activation observed could have been attributed to competition between C/EBPbeta and GR for TIF1beta .

Results from the deletion analysis indicated that the RBCC domain and the PHD domain-bromodomain are essential for the function of TIF1beta . The RBCC domain is the C/EBPbeta -interacting domain. Although the actual functional significance of these domains is unknown, it is currently assumed that they are involved in protein-protein interactions. The RBCC motif has been found in the N-terminal part of several putative transcriptional factors, ribonucleoproteins, and proto-oncogene products, including PML, RFP, RPT-1, SS-A/Ro, XNF7, and PWA33 (for a review, see reference 21). Three RBCC domain-containing proteins, PML, RFP, and TIF1alpha , have been identified in the context of fusion oncoproteins resulting from chromosomal translocations. The RBCC domain is fused to truncated products of other genes (23, 36, 54). Mutations in the RBCC domain of PML prevent PML nuclear body formation (11). Many PHD finger-containing proteins have been implicated in interactions between chromosomal proteins (1). These include products of the Drosophila genes trithorax and polycomblike. It is interesting to note that bromodomains are found in the adaptor proteins p300, CBP, and GCN5, as well as in SWI/SNF2 (13, 30, 35). These proteins reside in large multiprotein complexes. Thus, the overall structure of TIF1beta implies that it activates gene transcription by taking part in the formation of multiprotein complexes.

A TIF1beta -related protein, TIF1alpha , was found to interact with several nuclear hormone receptors. TIF1alpha contains a nuclear receptor-binding motif, LXXLL (28, 37). However, there is no motif resembling LXXLL in the TIF1beta sequence. Inhibition of RXRalpha activity was observed as a result of ectopic expression of TIF1alpha in the transient transfection assays. The RBCC domain-containing protein PML exerts a very powerful enhancing effect on the transactivating properties of several steroid hormone receptors (25). It is likely that a specific functional interaction exists between coactivators and nuclear receptors. The activation effect of TIF1alpha or TIF1beta on GR- or RXRalpha -induced gene expression, respectively, remains to be studied.

In addition to GR and C/EBPbeta , TIF1beta has been reported to interact with the transcriptional silencing domain of the Drosophila Kruppel-related KRAB proteins and to serve as a corepressor (22, 33, 43). The mechanism of repression by TIF1beta remains elusive. To test the possibility that TIF1beta is a general mediator of various transcriptional factors, we performed cotransfection assays with mammalian cell expression vectors for CREB and the protein kinase A catalytic subunit in the presence of the pC/EBPbeta -CAT reporter. TIF1beta could not enhance CREB activity (Fig. 2C). Another member of the C/EBP family, C/EBPalpha , was also tested, and the results showed that TIF1beta could augment the C/EBPalpha -activated expression of AGP (WT)-CAT (Fig. 2C). Thus, in addition to C/EBPbeta , the transcriptional activity of another member of the C/EBP family may be modulated by TIF1beta .

How could TIF1beta function as a coactivator? TIF1beta seems to be a bifunctional protein involved in the remodeling of the chromatin template in both the repression and the activation of transcription (37). Thus, the interaction between GR and TIF1beta or between C/EBPbeta and TIF1beta may promote the conversion of a transcriptionally inactive heterochromatin-like structure to an active euchromatin-like open structure by triggering the release of HP1 and MOD1 (37). We previously identified a nucleolar phosphoprotein, Nopp140, that functions as a mediator between C/EBPbeta and the general transcription factor TIFIIB (40). In light of the analogous features shared by the interactions between C/EBPbeta and Nopp140 or TIF1beta , we also tested the physical interaction between TIF1beta and TFIIB. Our results did not offer conclusive evidence on any physical interaction between TIF1beta and TFIIB (data not shown). Thus, the mechanism of activation of the agp gene by C/EBPbeta and TIF1beta is different from that of C/EBPbeta and Nopp140.

The present results revealed a direct protein-protein interaction between TIF1beta and GR and showed that this interaction is ligand independent. However, the functional interaction between GR and TIF1beta is ligand dependent. It is speculated that TIF1beta may participate in the formation of a coactivator complex to activate target gene expression. CBP has been demonstrated to interact with other nuclear receptor coactivators (SRC-1/ACTR/pCIP family) to form a functional complex and to result in a synergistic response to the nuclear receptors (19, 31, 57). These coactivators have been identified as histone acetyltransferases that remodel chromatin structure to facilitate transcriptional activation (19, 46, 53). Further experiments to identify other TIF1beta -interacting proteins may yield important mechanistic insights.

    ACKNOWLEDGMENTS

This research was supported by grants NSC86-2311-B001-089 and NSC88-2311-B001-114 (to C.-J.C.) and NSC86-2311-B001-094-Y (to S.-C.L.) from the National Science Council.

We thank Susan Taylor, Sophia Tasi, and Ming-Jer Tasi for plasmids and Bertrand Chin-Ming Tan for critical reading of the manuscript.

    FOOTNOTES

* Corresponding author. Mailing address: Institute of Molecular Medicine, College of Medicine, National Taiwan University, #7 Chun Shan South Rd., Taipei, Taiwan. Phone: 886-2-2356-2982. Fax: 886-2-2321-0977. E-mail: slee{at}ccms.ntu.edu.tw.

    REFERENCES
Top
Abstract
Introduction
Materials & Methods
Results
Discussion
References

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