INTRODUCTION

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p53 is the most frequently mutated human tumor suppressor gene identified to date. In model systems, restoration of p53 function in tumor cells leads either to a cell cycle block or to apoptosis. These activities have been linked to the ability of p53 to bind to specific DNA sequences and activate transcription. Among the genes that are activated by p53 are the cyclin-dependent kinase inhibitor p21 and a family of genes involved in oxidative stress (10, 36).

Recently, Kaghad et al. serendipitously identified a cDNA encoding a novel p53-like protein (22). The protein, called p73, is approximately 60% identical to p53 in the region corresponding to the p53 DNA-binding domain. Furthermore, all of the residues that contact DNA in the p53-DNA crystal structure are conserved in p73 (7). In addition, the N terminus of p73 is similar (~25% identity) to the N-terminal transactivation domain of p53, and the C terminus of p73 contains a region which is comparably similar to a C-terminal oligomerization domain within p53 (34, 44, 48). Finally, the genomic organizations of p53 and p73 are highly similar, suggesting that they have a common ancestry (22). In keeping with this high degree of similarity, p73 can, at least when overproduced, activate transcription from p53-responsive promoters and induce tumor cell apoptosis (20, 22).

The p73 gene maps to chromosome 1p36, a region that is frequently deleted in a variety of human cancers (22). Nonetheless, to date no mutations have been identified in the remaining p73 allele in such tumors. Thus, p73, unlike many tumor suppressor gene products such as the retinoblastoma protein (pRB) and p53, does not appear to conform to a two-hit model of tumorigenesis. It has been suggested that this may be due to epigenetic silencing of the p73 allele retained in tumors (22). Alternatively, it is possible that p73 is not a tumor suppressor gene product and is not the relevant target of 1p36 deletions.

A number of unrelated DNA tumor viruses can inactivate pRB. Inactivation of pRB leads to derepression of E2F-responsive promoters, which, in turn, allows quiescent cells to enter S phase. In model systems, untimely derepression of E2F-responsive promoters can also lead to both p53-dependent and p53-independent apoptosis (17, 25, 35, 37, 43, 50). These viruses, however, encode a variety of antiapoptotic proteins. For example, polyomaviruses, papillomaviruses, and adenoviruses encode T, E6, and E1B 55K proteins, respectively, which bind to and inactivate p53 (1, 3, 8, 11, 16, 19, 26, 30, 32, 40, 42, 49, 51). Indeed, p53 was first identified as a cellular T-binding protein (26, 30). In this manner, the host DNA replication machinery is usurped for viral replication.

T, E6, and E1B each target different regions of p53. T binds to p53's core DNA-binding domain (27, 38), whereas E1B 55K binds to, and silences, p53's N-terminal transactivation domain (4, 23, 29, 51). E6 interacts with p53's core DNA-binding domain and C terminus and targets p53 for ubiquitin-dependent proteolysis (28, 31, 32, 39, 40). In short, viral oncoproteins have been valuable reagents for studying the structure and functions of p53. Given the apparent similarity of p53 and p73, we asked whether viral oncoproteins that inactivate p53 were likewise capable of inactivating p73. Surprisingly, none of these viral oncoproteins interact with p73. Thus, small DNA tumor viruses can discriminate between p53 and p73.

	MATERIALS AND METHODS

Top Abstract Introduction Materials & Methods Results Discussion References

Cell lines and transfections. SK-N-SH, SK-N-AS, SK-N-DZ, and IMR-32 neuroblastoma cells, SK-N-MC neuroepithelioma cells, and IM-9 myeloma cells were obtained from the American Type Culture Collection (Rockville, Md.) and were grown in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum at 37°C in the presence of 10% CO₂. COS monkey kidney cells, 293 human embryonic kidney cells, and SAOS2 osteosarcoma cells were from the laboratory stocks of David Livingston and were maintained in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum at 37°C in the presence of 10% CO₂. Transfections were performed by the N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES)-buffered saline (BBS) method as described earlier (21). Briefly, cells growing in monolayers were transfected when approximately 70 to 80% confluent with 20 to 30 µg of plasmid DNA. The DNA precipitates were removed 18 h later and the cells were fed fresh medium. Protein and reporter assays were performed 24 h later.

Plasmids. The mammalian expression plasmids encoding hemagglutinin (HA)-tagged versions of simian p73 and p73 were described earlier (20). The human p53 cDNAs in pGEX2T-p53 (wild type) and pGEX2T-p53 (135Tyr) (18) (gifts of John Huibregtse and Peter Howley) were excised by digestion with BamHI and EcoRI and ligated into pcDNA3-HA that had been cut with these two enzymes to make pcDNA3-HAp53 and pcDNA3-HAp53(135Tyr), respectively.

Antibodies. Monoclonal antibodies were generated by standard techniques (14). RBF/DNJ (BnR) mice were immunized with either glutathione S-transferase (GST)-p73 (amino acids [aa] 380 to 637) for the ER antibodies or GST-p73 (aa 380 to 499) for the GC antibodies. The antibodies were screened based on their ability to immunoprecipitate ³⁵S-radiolabelled p73 ( and ) translation products and their ability to detect the original GST immunogens by Western blot analysis. ER13, ER15, and GC15 were selected for further characterization as described in Results.

Immunoprecipitation and immunoblot analysis. To detect endogenous p73, cells growing in monolayers were removed from plastic dishes by scraping and collected, along with floating cells, by centrifugation. The cells were then washed once with ice-cold phosphate-buffered saline and lysed, except where indicated, in EBC buffer (50 mM Tris [pH 8.0], 120 mM NaCl, 0.5% Nonidet P-40) supplemented with aprotonin (11.5 µg/ml), phenylmethylsulfonyl fluoride (50 µg/ml), NaF (100 mM), and Na orthovanadate (0.2 mM). In some experiments the cells were lysed in Triton 1% buffer (25 mM Tris [pH 7.4], 25 mM NaCl, 1% Triton X-100) or radioimmunoprecipitation assay buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1.0% Nonidet P-40, 0.5% deoxycholate, 0.1% sodium dodecyl sulfate [SDS]) supplemented with the same inhibitors. For some transfection experiments, cells were lysed directly in EBC buffer without scraping. Lysates were clarified by centrifugation at 14,000 × g for 15 min at 4°C. Protein concentrations were determined by the Bradford method.

In vitro translation. In vitro translation was performed with the TNT translation system (Promega) according to the manufacturer's protocol.

Electrophoretic mobility shift assay. A 23-mer oligonucleotide corresponding to a wild-type (5'-AGCTTAGGCATGTCTAGGCATGTCTA-3') p53 DNA-binding site (10) was synthesized, annealed to a complementary synthetic oligonucleotide in vitro, and radiolabelled with [-³²P]ATP by using polynucleotide kinase (New England Biolabs). DNA binding reactions were performed in 20 µl containing 2 µl of the indicated protein in vitro translation product (or unprogrammed reticulocyte lysate), 10 µl of 2× DNA binding buffer (50 mM Tris [pH 7.5], 40% glycerol, 100 mM KCl, 100 mM dithiothreitol, 2 mg of bovine serum albumin per ml, and 0.2% [vol/vol] Triton X-100), 1 ng of radiolabelled p53 DNA-binding site, and 300 ng of salmon sperm DNA. Where indicated, 40 ng of nonlabelled wild-type or sequence-scrambled (sense strand, 5'-CCAGCTAGCAGGCAGCATCAGTACTT) duplex oligonucleotides was added as specific and nonspecific competitors, respectively. Antibody supershift experiments were performed by adding 2 µl of a 1:10 (vol/vol) dilution of the indicated hybridoma supernatant. Binding reaction mixtures were incubated for 15 min at room temperature and then resolved by electrophoresis at 4°C in a 4% nondenaturing polyacrylamide gel containing 0.5× Tris-borate-EDTA buffer. Electrophoresis was conducted for 2 to 3 h at 200 V. The gels were then dried and exposed to film at 70°C with an intensifying screen.

p53 degradation assay. The E6 destruction and GST-E6 binding assays were performed as described elsewhere (1, 31, 40).

CAT assay. SAOS2 cells were transiently transfected with 5 µg of reporter plasmid, 2 µg of pCMV-gal, 0.5 µg of p73 or p53 expression plasmid, and 10 µg of wild-type or mutant large-T-antigen plasmid. The total amount of DNA was brought up to 24 µg with pRcCMV carrier plasmid (Invitrogen). At 48 h after transfection, chloramphenicol acetyltransferase (CAT) assays were performed as described previously (21).

	RESULTS

Top Abstract Introduction Materials & Methods Results Discussion References

In order to study the p73 protein, we generated monoclonal anti-p73 antibodies. Two alternatively spliced p73 mRNA isoforms, designated p73 and p73, were identified by Kaghad et al. (22). The p73 isoform lacks exon 13. Consequently, p73 contains a frameshift and terminates prematurely after the addition of a 5-aa sequence not present in the alpha isoform. Bacterial expression plasmids that encoded GST fused to the C terminus of either simian p73 or p73 were made (Fig. 1). These fusion proteins, which were designed so as to minimize the likelihood of generating antibodies that would cross-react with p53, were then used to immunize mice. Hybridomas were generated by standard techniques, and monoclonal antibodies were screened based on their ability to immunoprecipitate p73, but not p53, in vitro translation products. At least three such monoclonal anti-p73 antibodies, named ER13, ER15, and GC15, were identified in this fashion.

View larger version (20K): [in this window] [in a new window]   FIG. 1.   GST-p73 fusion proteins used to generate monoclonal antibodies. Shown schematically are p53, p73, and p73. Mice were immunized with GST fused to either the C terminus of p73 (ER mice) or the C terminus of p73 (GC mice). The p73 cDNA lacks exon 13, resulting in a frameshift at residue 495. p73 therefore contains five residues (black box) not present in p73.

ER13 bound to p73, but not p73, in immunoprecipitation and immunoblot assays (Fig. 2A and D), suggesting that the ER13 epitope is located within the C-terminal region that is unique to p73 (Fig. 1). Of note, however, ER13 did immunoprecipitate p73 in the presence of p73 under native, but not denaturing, conditions (Fig. 2B [compare lanes 3 and 7] and 3 and data not shown). This suggests that p73 and p73 can heterodimerize in solution. ER15 and GC15 bound to both p73 and p73 in immunoprecipitation and immunoblot assays (Fig. 2A and D). The ability of GC15 to immunoprecipitate p73, however, was diminished under denaturing conditions (Fig. 2A, compare lanes 6 and 10). This, coupled with the different band patterns observed in the immunoblot assays shown in Fig. 2D, implies that ER15 and GC15 recognize different epitopes common to p73 and p73 (note that multiple bands are seen in Fig. 2D due to limited proteolysis of GST fusion proteins during their production and purification). None of the anti-p73 antibodies recognized p53 or the recently identified p53-related protein p51/Ket (33, 41, 46) (Fig. 2C and D).

View larger version (58K): [in this window] [in a new window]   FIG. 2.   Characterization of anti-p73 antibodies. (A) p73 and p73 35S-labelled in vitro translation products were immunoprecipitated with either control (anti-T; PAb419) or anti-p73 (ER13, ER15, and GC15) antibodies, as indicated, under native (lanes 3 to 6 and 11 to 14) or denaturing (lanes 7 to 10 and 15 to 18) conditions. Two microliters of the indicated in vitro translation product was loaded directly in lanes 1 and 2. (B) p73 and p73 were cotranslated in vitro in the presence of [35S]methionine and immunoprecipitated with either control (anti-T; PAb419) or anti-p73 (ER13, ER15, and GC15) antibodies, as indicated, under native (lanes 2 to 5) or denaturing (lanes 6 to 10) conditions. Two microliters of the indicated in vitro translation product was loaded directly in lanes 1, 11, and 12. (C) p51A, p53, and p73 35S-labelled in vitro translation products were immunoprecipitated with either control (anti-T; PAb419) or anti-p73 (ER13, ER15, and GC15) antibodies, as indicated. Two microliters of the indicated in vitro translation product was loaded directly in lanes 1 to 3. For panels A to C, proteins were resolved by SDS-polyacrylamide gel electrophoresis and detected by fluorography. (D) GST-p53 (lanes 1 to 5), GST-p73 (aa 380 to 499) (lanes 6 to 10), and GST-p73 (aa 380 to 637) (lanes 11 to 15) were produced in bacteria, purified by using glutathione-Sepharose, resolved by SDS-polyacrylamide gel electrophoresis in preparative wells, and transferred to a membrane. The membrane was cut into strips and immunoblotted with either control (anti-T; PAb419) or anti-p73 (ER13, ER15, and GC15) antibodies, as indicated. Bound antibody was detected colorimetrically with an alkaline phosphatase-conjugated antimouse antibody.

View larger version (34K): [in this window] [in a new window]   FIG. 3.   Identification of p73 and p73 in cell extracts. (A) COS cells were lysed in the presence of 0.5% Nonidet P-40 (EBC buffer) (lanes 1 to 3), in radioimmunoprecipitation assay buffer (lanes 4 to 6), or in the presence of 1% Triton (lanes 7 to 9) and immunoprecipitated (IP) with a control antibody (anti-T [PAb419]), anti-p73 (ER13), or anti-p73 (GC15) as indicated. Antibodies were cross-linked to protein A-Sepharose prior to immunoprecipitation. (B) COS cells (lanes 1 to 4) and SK-N-AS cells (lanes 5 to 8) were lysed in EBC buffer and immunoprecipitated with a control antibody (anti-T [PAb419]), anti-p73 (ER13), anti-p73 (ER15), or anti-p73 (GC15) as indicated. For both panels, Bound proteins were resolved by electrophoresis in an SDS-8% polyacrylamide gel and immunoblotted with anti-p73 (ER15) by using a horseradish peroxidase-conjugated antimouse antibody and enhanced chemiluminescence.

It has not been formally proven that mammalian cells produce both p73 and p73 proteins. To address this, COS monkey kidney cells were lysed with a variety of detergents and immunoprecipitated with anti-p73 antibodies, or a control antibody, under conditions of antibody excess (Fig. 3A, lanes 1 to 9; Fig. 3B, lanes 1 to 4). Specifically bound proteins were detected by immunoblot analysis with an anti-p73 antibody (ER15). Two proteins, with apparent molecular masses of ~80 and 70 kDa, were detected in the anti-p73, but not control, immunoprecipitates. These two proteins comigrated with the in vitro translation products of simian p73 and p73 cDNAs, respectively, under a variety of SDS-polyacrylamide gel electrophoresis conditions (data not shown). As an additional specificity control, SK-N-AS human neuroblastoma cells, which do not produce detectable levels of p73 mRNA (22), were analyzed in parallel (Fig. 3B, lanes 5 to 8). As expected, these cells lacked the 80- and 70-kDa species (Fig. 3B, compare lanes 6 to 8 with lanes 2 to 4). We have thus far been unable to purify enough of the 80- and 70-kDa species to perform partial proteolytic peptide mapping or to obtain peptide sequence data. Nonetheless, the facts that they (i) are recognized by multiple, independent, anti-p73 monoclonal antibodies, (ii) comigrate with p73 and p73 in vitro translation products, and (iii) are absent in a cell line that lacks p73 mRNA strongly suggest that they are p73 and p73, respectively.

p73 maps to chromosome 1p36, a region that is frequently deleted in a variety of human tumors, including neuroblastomas (5, 45, 47). Although no intragenic p73 mutations have been identified to date, it has been suggested that the p73 gene locus might be subject to epigenetic regulation. In the next set of experiments, whole-cell extracts were prepared from COS cells, IM9 human myeloma cells, and a panel of neuroblastomas and neuroepitheliomas (Fig. 4). These extracts were resolved by SDS-polyacrylamide gel electrophoresis and immunoblotted with an anti-p73 antibody (ER15). p73 and p73 were readily detected in both COS cell extracts and IM9 cell extracts (Fig. 4, lanes 1 and 2). Thus, the failure to detect p73 and p73 in SK-N-AS cells (Fig. 3B, lanes 6 to 8, and 4, lane 5) cannot be attributed to species differences. p73 and p73 were either absent or barely detectable in all of the neuroblastoma and neuroepithelioma cell lines tested (Fig. 4, compare lanes 4 to 8 with lanes 1 and 2). Some of these cell extracts, including those prepared from COS, SK-N-SH, and SK-N-DZ cells, contained faster-migrating species that reacted with the ER15 antibody (data not shown). Whether these proteins represent additional p73 isoforms, additional p53/p73 family members, or unrelated proteins that share the ER15 epitope is currently under investigation. We find that most human tumor cell lines, like the neuroblastoma cell lines studied here, produce low or undetectable levels of p73 and p73 (unpublished data).

View larger version (42K): [in this window] [in a new window]   FIG. 4.   p73 and p73 protein levels in neuroblastoma cell lines. Cell extracts prepared from the indicated cell lines were resolved by electrophoresis in an SDS-8% polyacrylamide gel and immunoblotted with an anti-p73 antibody (ER15). Each lane contained ~300 µg of total cell extract. Bound antibody was detected by using a horseradish peroxidase-conjugated antimouse antibody and enhanced chemiluminescence.

The availability of anti-p73 antibodies allowed us to next test directly whether p73 could, as predicted, bind to canonical p53 DNA-binding sites. A radiolabelled p53 DNA-binding site was incubated with either p53 (Fig. 5A, lanes 4 to 9) or p73 (lanes 10 to 15) synthesized in vitro by using a rabbit reticulocyte lysate. Unprogrammed rabbit reticulocyte lysate served as a negative control (lanes 1 to 3). As expected, p53 bound to this site (Fig. 5A, lane 4, arrow). This complex was specific, as it was abolished in the presence of excess, unlabelled competitor DNA that contained p53-binding sites and was unaffected by a competitor DNA that lacked such sites (Fig. 5A, compare lanes 6 and 5 to lane 4). In contrast, the complex indicated by the asterisk, detectable even with unprogrammed reticulocyte lysate, was abolished by both specific and nonspecific competitor DNA. The complex labelled p53/DNA contained p53, as it was supershifted by an anti-p53 antibody (Fig. 5A, lane 7) but not by anti-p73 or control (anti-T) antibodies (lanes 8 and 9). Similarly, p73 formed a specific complex with DNA that was supershifted by an anti-p73 antibody but not by the anti-p53 and anti-T antibodies (Fig. 5A, lanes 10 to 15). Comparable results were obtained with p73 (Fig. 5B, compare lanes 1 to 5 with lanes 6 to 10). Thus, as predicted, p73 and p73 can bind to p53 DNA-binding sites in a specific manner.

View larger version (56K): [in this window] [in a new window]   FIG. 5.   p73 binds to canonical p53 DNA-binding sites. A 32P-radiolabelled p53 DNA-binding site was incubated with p53 in vitro translation product (A, lanes 4 to 9), p73 in vitro translation product (A, lanes 10 to 15, and B, lanes 1 to 5), p73 in vitro translation product (B, lanes 6 to 10), or unprogrammed reticulocyte lysate (A, lanes 1 to 3) and subjected to electrophoretic mobility shift analysis. Binding reactions were carried out in the presence of a vast molar excess of unlabelled specific or nonspecific competitor DNA where indicated. Anti-p53, anti-p73, or a control antibody (anti-T) was added prior to the electrophoretic mobility shift assay as indicated. The asterisk indicates a nonspecific complex.

These results, together with earlier reports, suggest that p73 can, at least when overproduced, mimic the ability of p53 to bind to DNA, to activate transcription, and to inhibit tumor cell growth. DNA tumor viral oncoproteins have been invaluable reagents for elucidating the normal functions of tumor suppressor proteins, such as pRB and p53. Simian virus 40 (SV40) T antigen binds to the central region of p53 responsible for DNA binding (27, 38). The adenovirus E1B 55K protein interacts with residues within the N-terminal p53 transactivation domain that are highly conserved between p73 and p53 (4, 23, 29, 51). The human papillomavirus E6 protein interacts with the C terminus and the core structure of p53 (18, 31, 32, 39, 40). Given the high degree of similarity between p53 and p73, we next asked whether these viral oncoproteins could likewise physically interact with p73.

293 human embryonic kidney cells are stably transformed with a fragment of the adenovirus genome and produce E1B 55K (12). In the first set of experiments, these cells were immunoprecipitated with an anti-E1B 55K or control monoclonal antibody (Fig. 6A, lanes 2 and 1, respectively). The immunoprecipitates were resolved by SDS-polyacrylamide gel electrophoresis and immunoblotted with an anti-p73 antibody (left panel) or anti-p53 antibody (right panel). Anti-p73 and anti-p53 immunoprecipitates prepared in parallel served as additional controls (lanes 4 and 3, respectively). As expected, p53 coimmunoprecipitated with E1B 55K (Fig. 6A, lane 2, right panel). In contrast, p73 was not detectable in anti-E1B 55K immunoprecipitates (Fig. 6A, lane 2, left panel) despite the presence of p73 in these cells (Fig. 6A, lane 4, left panel).

View larger version (28K): [in this window] [in a new window]   FIG. 6.   Differential binding of p53 and p73 to E1B 55K. (A) 293 human embryonic kidney cells were lysed and immunoprecipitated (IP) with the indicated antibodies. Specifically bound proteins were resolved by electrophoresis in an SDS-10% polyacrylamide gel and detected by immunoblotting with anti-p73 antibody (left panel) or anti-p53 antibody (right panel). Bound antibodies were detected by using a horseradish peroxidase-conjugated antimouse antibody and enhanced chemiluminescence. The asterisk indicates faster-migrating species, previously noted by others, that interact with anti-p53 antibodies. (B) 293 human embryonic kidney cells were transfected with 1 or 10 µg, as indicated by the triangles, of expression plasmids encoding HA-tagged p53 (lanes 2, 7, and 8) or HA-tagged p73 (lanes 4, 5, 9, and 10) or with 10 µg of the backbone expression plasmid (lanes 1 and 6). Cell extracts were prepared and immunoprecipitated with anti-HA (lanes 1 to 5) or anti-E1B 55K antibodies (lanes 6 to 10). Specifically bound proteins were resolved by electrophoresis in an SDS-10% polyacrylamide gel and detected by immunoblotting with anti-HA antibody (12CA5) by using a horseradish peroxidase-conjugated antimouse antibody and enhanced chemiluminescence. The asterisks indicate nonspecific bands.

These results left open the possibility that the failure to detect an association between p73 and E1B 55K reflected differences in the abundances of p73 and p53 in these cells. To address this concern, 293 cells were transiently transfected with an empty expression plasmid (Fig. 6B, lanes 1 and 6) or plasmids encoding HA-tagged p53 (lanes 2, 3, 7, and 8) or p73 (lanes 4, 5, 9, and 10). Cell extracts were prepared and immunoprecipitated with anti-HA antibody (lanes 1 to 5) or anti-E1B 55K (lanes 6 to 10) under conditions of antibody excess. Bound proteins were detected by anti-HA immunoblot analysis. Both p73 and p53 were produced at comparable levels (compare lanes 2 and 3 to lanes 4 and 5, respectively). Despite this, p53 was readily detected in anti-E1B 55K immunoprecipitates, whereas p73 was not (compare lane 7 to lane 9). Thus, we have been unable to detect stable complex formation between E1B 55K and p73. Similar results were obtained for p73 (data not shown).

To determine whether E6 could interact with p73, we first performed GST pull-down assays with GST-E6 (Fig. 7A). As expected, radiolabelled p53 bound to GST-E6, whereas p73 and p73 did not (compare lanes 4 to 6 with lanes 7 to 12). None of these proteins bound to GST alone (lanes 13 to 15). Next we used an in vitro degradation assay developed by Scheffner et al. (40). This assay exploits the fact that rabbit reticulocyte lysate contains all of the proteins required for ubiquitin-dependent proteolysis of p53. Coincubation of bacterially produced E6 with radiolabelled p53 in vitro translation product led to virtual disappearance of the latter within 30 min (Fig. 7B, compare right and left panels). In contrast, under identical assay conditions, recombinant E6 had no discernible effect on the stability of p73 or p73 (Fig. 7B, compare right and left panels). Thus, E6 can not physically interact with p73 and is unable to target it for ubiquitin-dependent proteolysis.

View larger version (59K): [in this window] [in a new window]   FIG. 7.   E6 targets p53, but not p73, for degradation in vitro. (A) p53, p73, and p73 35S-labelled in vitro translation products were incubated with immobilized GST-E6 (lanes 4 to 12) or GST (lanes 13 to 15) as indicated. Each binding reaction mixture contained 40 µl (lanes 4, 7, 10, and 13 to 15), 20 µl (lanes 5, 8, and 11), or 10 µl (lanes 6, 9, and 12) of in vitro translation product. Specifically bound proteins were resolved by SDS-polyacrylamide gel electrophoresis and detected by fluorography. Five microliters of the indicated in vitro translation product was loaded directly in lanes 1 to 3. (B) p53, p73, and p73 35S-labelled in vitro translation products were incubated at 30°C with a lysate prepared from bacteria producing GST-E6 (right panel) or GST-E4 (left panel). The proteins were then resolved by electrophoresis in an SDS-10% polyacrylamide gel and detected by fluorography.

T antigen binds to the region that is most highly conserved between p53 and p73 (27, 38). This region has been implicated in p53's ability to bind to specific DNA sequences and is also frequently altered in human tumors (2, 15, 34, 48). p73 was first identified in COS cells, a cell line that stably produces T antigen. We noted, however, that p73 was not present in anti-T immunoprecipitates prepared from these cells (Fig. 3A, lanes 1, 4, and 7, and Fig. 3B, lane 1), raising the possibility that T antigen does not bind to p73. To address this further, COS cells were transiently transfected with plasmids encoding HA-tagged versions of wild-type p53, a tumor-derived p53 mutant (p53 135Tyr) (6), p73, and p73 or with the empty vector (Fig. 8A). Cell extracts were prepared and immunoprecipitated with anti-HA antibody (lanes 1 to 5) or anti-T antibody (lanes 6 to 10). Bound proteins were resolved by SDS-polyacrylamide gel electrophoresis and immunoblotted with an anti-HA antibody. All of the ectopically expressed proteins were produced at comparable levels (compare lanes 2 through 5). As expected, T antigen bound to wild-type p53 and, to a lesser extent, to the tumor-derived p53 mutant (lanes 7 and 8). In contrast, p73 was not detectable in the anti-T immunoprecipitates (lanes 9 and 10).

View larger version (21K): [in this window] [in a new window]   FIG. 8.   Differential binding of p53 and p73 to SV40 T. (A) COS cells, which stably produce SV40 T, were transfected with expression plasmids encoding the indicated HA-tagged proteins or with the backbone expression plasmid (Mock). Cell extracts were prepared and immunoprecipitated (IP) with anti-HA (lanes 1 to 5) or anti-T (lanes 6 to 10) antibodies. Specifically bound proteins were resolved by electrophoresis in an SDS-10% polyacrylamide gel and detected by immunoblotting with anti-HA antibody (12CA5) by using a horseradish peroxidase-conjugated antimouse antibody and enhanced chemiluminescence. (B) SAOS2 cells were transfected a CAT reporter plasmid containing a minimal promoter consisting of two p53-binding sites upstream of a TATA box together with expression plasmids for wild-type p53 or p73. In each case, the amount of CAT activity, in the absence of T antigen, was set to 100%. Where indicated, the cells were also transfected with a plasmid encoding wild-type (wt) or mutant (mut) T antigen. Error bars represent one standard deviation.

In the final set of experiments, SAOS2 cells were transfected with a reporter plasmid in which the CAT gene was placed under the control of a p53-responsive promoter (2XRGC-CAT) along with expression plasmids encoding either p53 or p73 (Fig. 8B). These cells have both copies of the p53 gene deleted and consequently do not contain p53 protein (9). In addition, they contain either no or barely detectable levels of p73 (reference 22 and data not shown). In keeping with earlier studies, both p53 and p73 activated this reporter (20). Coexpression of wild-type T, but not a T-antigen mutant (dl357-370) that is incapable of binding to p53 (24), reproducibly inhibited the ability of p53 to activate this reporter. In contrast, neither wild-type T nor the T mutant significantly inhibited p73 function in these assays. Note that these experiments were performed with a 20-fold excess of T-antigen expression plasmid relative to the p53 or p73 expression plasmid. Similar results were obtained when a reporter containing the p53-responsive p21 promoter was used instead of 2XRGC-CAT (data not shown). In summary, both the biochemical data and transcriptional activation data suggest that T antigen, like E1B and E6, discriminates between p53 and p73.

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

Viral oncoproteins, as well as high-affinity monoclonal antibodies, have proven to be invaluble reagents for studying the functions of tumor suppressor proteins. In this report, we have described the generation and validation of multiple anti-p73 monoclonal antibodies. Using these reagents, we have confirmed that the two previously recognized p73 isoforms are produced in mammalian cells and can, as predicted, bind to canonical p53 DNA-binding sites.

To date no intragenic p73 mutations have been identified in human cancer cells. It has been suggested, however, that p73 is monoallelically expressed (22). If this was true, then loss of the transcriptionally active allele, as a result of a chromosomal deletion, might contribute to human carcinogenesis. On the other hand, others have found evidence of biallelic p73 expression in human T cells. It remains to be determined whether monoallelic expression of p73 might be restricted to certain tissues or whether the ability to imprint the p73 locus might itself be a polymorphic trait. p73 transcript analysis is further complicated by the existence of alternative promoters as well as of alternatively spliced mRNAs (4a). In summary, while a role for epigenetic control of p73 expression cannot be excluded, there are currently no genetic data that firmly establish that p73 is a tumor suppressor gene product.

At the protein level, we found that many tumor cell lines, including neuroblastoma cell lines, produce low levels of p73 and p73. In the absence of p73 gene mutations, however, it is impossible to know whether this reflects pathology or physiology. Thus, one possibility is that p73 and p73 are, indeed, suppressed epigenetically. Alternatively, it is possible that the precursor cells that give rise to these tumors normally produce low levels of p73 and p73. In addition, it is possible that p73 levels fall as a result of, rather than as a cause of, the malignant transformation of these cells.

One would have predicted that viral oncoproteins that inactivate p53 would also inactivate p73 if p53 and p73 played similar roles in cells. A potential precedent would be the inactivation of multiple retinoblastoma protein family members by adenovirus E1A, SV40 T, and human papillomavirus E7. Instead, we were unable to detect stable complexes between these proteins and p73 by using both biochemical and functional assays. One possible explanation is that p73 is not produced in those cells that are susceptible to transformation by these viruses. We found, however, that p73 is produced in COS cells and 293 cells. These cell lines are transformed by T and the left half of the adenovirus genome, respectively. Thus, stable binding of T or E1B 55K to p73 is apparently not necessary for transformation.

The high degree of similarity to p53 might have led to viral oncoprotein binding even if, as suggested above, inactivation of p73 is not required for transformation. In other words, if not by natural selection, then p73 might have bound to adenovirus E1B 55K, SV40 T, or human papillomavirus E6 by chance. The observation that viral oncoproteins discriminate between p53 and p73 raises the possibility that preservation of one or more p73 functions facilitates viral replication. In this light, the high levels of p73 seen in COS cells and 293 cells raise two interesting possibilities. The first is that high levels of p73 render a cell permissive for viral replication or transformation. The second is that p73 is induced following transformation with these agents.

In summary, despite the similarity between p53 and p73, only the former is a recurrent target of mutations in human cancer and a direct target of viral oncoproteins. This suggests that p53 and p73 are not functionally equivalent. In this regard, experiments performed to date suggest that p73, unlike p53, is not induced by DNA damage. Thus, p53 may have uniquely evolved to respond to genotoxic stress. Clearly, additional experiments are needed to decipher the normal functions of p73 and to determine whether it plays a role in human carcinogenesis.