INTRODUCTION

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Ras proteins are small guanine nucleotide binding proteins that play a central role in signal transduction pathways that regulate cell proliferation (2). Wild-type Ras proteins are activated transiently, via guanine nucleotide exchange mechanisms, in response to a wide variety of extracellular signalling agents (3). When in the GTP-bound state, Ras is capable of binding to several different established and potential effector proteins, including members of the Raf, phosphatidylinositol 3 (OH)-kinase [PI(3)K], and Ral guanine nucleotide dissociation stimulator (RalGDS) families, Rin, protein kinase C, AF6, and the GTPase-activating proteins p120^GAP and neurofibromin (17, 28). Binding to Ras leads, directly or indirectly, to activation of these effectors, which in turn activate downstream signalling cascades. Thus, Ras may be viewed as a hub from which multiple pathways radiate. Activating mutations in Ras result in constitutive signalling to these downstream elements, and such mutations are observed with high frequency in human tumors (4).

Expression of mutated, oncogenic Ras in cultured rodent fibroblast cell lines induces a highly pleiotropic response, including alterations in cell morphology, loss of contact inhibition, changes in gene expression, decreased dependence on serum growth factors, and the ability to proliferate in the absence of adhesion to a substratum (i.e., anchorage-independent growth). Many of these phenotypes can be dissociated from one another. For example, introduction of Ras oncoprotein into quiescent Swiss 3T3 cells led to both morphological transformation and DNA synthesis, but only the induction of DNA synthesis required activation of protein kinase C (27). Furthermore, a Rat 6 fibroblast-derived mutant cell line, ER-1-2, responded to stable expression of the v-H-ras oncogene with alterations in morphology and gene expression that were nearly indistinguishable from those observed with a matched control cell line yet failed to form colonies in soft agar in response to ras (11, 22). Similarly, expression of a dominant negative form of Rac1 in Ras-transformed Rat 1 cells inhibited anchorage-independent growth of these cells but had only marginal effects on their transformed morphology (34).

The studies cited above raise the possibility that different aspects of the transformed phenotype might be controlled by distinct combinations of Ras-regulated pathways. Evidence for this notion has been elegantly provided through the use of Ras effector loop mutants. Certain point mutations in this region (amino acids 32 to 40 in H-Ras) render Ras defective for binding specific effector proteins while remaining competent for binding and activating others, albeit at lower than wild-type efficiency (39, 46). Several individual mutants were defective in transformation assays but, when coexpressed, complemented each other (19, 39, 46). These and additional studies have implicated at least three effector proteins as potential synergistic mediators of transformation by Ras: Raf, PI(3)K, and RalGDS (15, 19, 20, 33, 39, 46, 47). Raf stimulates the mitogen-activated protein kinase (MAPK)/Erk cascade, leading to phosphorylation and activation of transcription factors and other proteins (29). PI(3)K stimulates cortical actin rearrangement via the small GTP binding protein Rac (39). Ras also activates the c-Jun N-terminal kinase cascade in a Rac-dependent manner (30), but the role of PI(3)K in Ras-mediated activation of this pathway is not yet established. The mechanisms by which RalGDS contributes to the transformed phenotype are not known but may involve both Ral-dependent and -independent pathways (33, 45, 47). It should be mentioned that Raf, MAPK, PI(3)K, and Rac are each required for Ras to exert its full powers of transformation (18, 21, 35, 39) and, under certain conditions, transform fibroblasts on their own (6, 9, 18, 44).

We have undertaken an analysis of the mechanisms by which Ras inappropriately drives cell cycle events to induce anchorage-independent growth. Anchorage-independent growth is the best in vitro correlate of tumorigenicity (42), but the effector pathway or pathways utilized by Ras to produce this phenotype are still largely unknown. We and others demonstrated that several important cell cycle events were dependent on cell-substratum adhesion of nontransformed fibroblast cell lines, including (i) activation of G₁ cyclin-dependent kinases (Cdks; as measured by cyclin D1- and E-dependent kinase activities and phosphorylation of pRB family members) and (ii) expression of the cyclin A gene (5, 10, 13, 16, 40, 51). Certain other aspects of the cell cycle, such as expression of cyclin E, were not regulated by adhesion. Strikingly, all of these cell cycle activities occurred in the absence of adhesion in Ras-transformed cell lines (5, 16). In contrast, ER-1-2 cells that expressed v-H-ras (ER-1-2/ras cells), which failed to proliferate in soft agar, possessed G₁ Cdk activities when cultured without adhesion but remained almost completely adhesion dependent for expression of cyclin A (16). Importantly, ectopic expression of cyclin A rescued anchorage-independent growth of ER-1-2/ras cells but did not induce anchorage-independent growth of control or ER-1-2 cells, presumably because these cells still lacked G₁ Cdk activities in the absence of adhesion (16). Anchorage-independent activation of G₁ Cdks and expression of cyclin A are therefore likely to be functionally relevant endpoints in determination of the transformed phenotype.

Expression of the cyclin A gene is dependent on G₁ Cdk activity (50). Results from the ER-1-2 cell system and other studies (7, 8, 16, 51) indicate, however, that there is an additional, adhesion- and Ras-regulated function that is also required for cyclin A expression and that this function is at least partly dissociable from the mechanisms by which adhesion and Ras regulate G₁ Cdk activity. It is possible, therefore, that multiple Ras effector pathways are required to supplant adhesion-mediated cell cycle regulation and induce growth in soft agar. To test this hypothesis more directly, we have stably expressed Ras effector loop mutants in Rat 6 and NIH 3T3 fibroblasts and analyzed the cells for (i) growth in soft agar and (ii) the ability to drive specific cell cycle events in the absence of cell-substratum adhesion. These studies indicate that Ras signals to the cell cycle machinery via several pathways and that a combination of pathways is required to produce efficient anchorage-independent growth.

	MATERIALS AND METHODS

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Cell culture. Rat 6- and NIH 3T3-derived cell lines were cultured in Dulbecco modified Eagle medium (Gibco) plus 10% bovine calf serum as previously described (16, 22). NIH 3T3 cells were obtained from T. Hei (Columbia University). This strain of NIH 3T3, which has a very low frequency of spontaneous focus formation, derives originally from a deposit by S. A. Aaronson to the Japanese Cancer Research Resources Bank. Soft agar assays were performed as described by Kang and Krauss (16). To recover cells cultured under nonadherent conditions, preparative methylcellulose cultures were used in place of soft agar cultures (16). Briefly, 10⁵ cells were inoculated into 10 ml of Dulbecco modified Eagle medium containing 5% calf serum and 1.3% methylcellulose in a 50-ml conical tube. The tubes were then placed in a water-jacketed CO₂ incubator at 37°C. Three days later, the medium was diluted with 40 ml of ice-cold phosphate-buffered saline (to solubilize the methylcellulose), and the cells were recovered by gentle centrifugation.

Transfections and retroviral infections. pDCR-ras(12V), pDCR-ras(12V35S), pDCR-ras(12V37G), and pDCR-ras(12V40C) encode the indicated mutant Ras alleles under the control of the cytomegalovirus promoter and also contain the selectable marker gene neo. These reagents were originally developed by White and colleagues (46) and were kindly provided by D. Bar-Sagi (State University of New York at Stony Brook). Rat 6 and NIH 3T3 cells were transfected with a total of 20 µg of these plasmids, either singly or in paired combination, by the calcium phosphate technique (48). Transfected cultures were selected in G418-containing medium (400 µg per ml for Rat 6-derived cell lines and 700 µg per ml for NIH 3T3-derived cell lines), and drug-resistant colonies were pooled and analyzed as described in Results. Two independent, paired combination transfections were performed for the Rat 6 cells, with indistinguishable results. Additionally, because cotransfection of the 12V,35S and 12V,40C constructs did not produce macroscopic colonies in Rat 6 cells, these plasmids were also transfected serially. The pooled transfectants that stably expressed the 12V,35S mutant were then retransfected with the 12V,40C construct along with a plasmid encoding hygromycin resistance. Transfected cultures were selected in hygromycin-containing medium (200 µg/ml), and resistant colonies were pooled and tested for growth in soft agar. The serial transfectants gave results that were very similar to those for the cotransfectants.

Immunoblotting. Cells from monolayer or methylcellulose suspension cultures were harvested in lysis buffer (50 mM Tris-HCl [pH 8.0], 250 mM NaCl, 1% Nonidet P-40, 2 mM EDTA) containing 1 mM phenylmethylsulfonyl fluoride, 10 ng of leupeptin per ml, 50 mM NaF, and 1 mM sodium orthovanadate. Total proteins were then separated on sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred to nitrocellulose membranes (Amersham), and the membranes were probed with specific antibodies. After extensive washing (with 40 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA), the blots were reprobed with horseradish peroxidase-conjugated secondary antibody, and specific protein bands were visualized with the Amersham ECL chemiluminescence detection system. Immunoblotting was performed with the following antibodies, from the indicated sources; anti-H-Ras (SC-35; Santa Cruz Biotechnology), anti-Erk2 (SC-154; Santa Cruz Biotechnology), anti-cyclin D (06-13T; UBI), anti-cyclin E (SC-481; Santa Cruz Biotechnology), anti-cyclin A (M. Pagano, New York University), anti-human cyclin A (clone 6E6; Novocastra Laboratories), anti-human cyclin E (06-134; UBI), anti-pRB (14001A; PharMingen), anti-p27^kip1 (A. Koff, Memorial Sloan-Kettering Cancer Center), and anti-p21^cip1 (SC-397; Santa Cruz Biotechnology).

Erk2 assays. Cells from methylcellulose suspension cultures were harvested in lysis buffer (50 mM HEPES [pH 7.6], 100 mM NaCl, 1% Nonidet P-40, 2 mM EDTA) containing 1 mM phenylmethylsulfonyl fluoride, 10 ng of leupeptin per ml, 50 mM NaF, and 1 mM sodium orthovanadate; 400 µg of cell lysate was immunoprecipitated with anti-Erk2 antibody (SC-154; Santa Cruz Biotechnology), and the precipitates were washed repeatedly and incubated in reaction buffer (10 mM HEPES, 10 mM magnesium acetate) containing 50 µM ATP, 5 µCi of [-³²P]ATP, and 40 µg of myelin basic protein in a final volume of 40 µl for 30 min at 30°C. The products of the reaction were separated on an SDS-15% polyacrylamide gel. The gel was then dried and exposed to X-ray film.

Cyclin E-dependent kinase assays. For in vitro cyclin E-dependent kinase assays, cyclin E was immunoprecipitated from 500 µg of total cellular protein (from lysates prepared as described above) with polyclonal anti-cyclin E antibody (SC-481; Santa Cruz Biotechnology), and the immunoprecipitates were washed three times with lysis buffer and twice with kinase buffer (20 µM Tris-HCl [pH 7.4], 4 µM MgCl₂). The washed immunoprecipitates were then incubated with kinase buffer, 2 µg of histone H1, 1 µM ATP, and 5 µCi of [-³²P]ATP in a final volume of 16 µl for 30 min at 37°C. The products of the reaction were separated on an SDS-12% polyacrylamide gel. The gel was then dried and exposed to X-ray film.

	RESULTS

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Phenotypic effects of Ras effector loop mutants on NIH 3T3 and Rat 6 cells. To test the hypothesis that multiple Ras effector pathways might contribute to the induction of anchorage-independent growth, the NIH 3T3 and Rat 6 cell lines were transfected with expression vectors for fully activated H-Ras (12V), three H-Ras effector loop mutants that also contained the activating 12V mutation (12V,35S, 12V,37G, and 12V,40C), or an empty vector as a control. Of the three effector proteins for which there is evidence of a role in Ras-mediated transformation, the 12V,35S mutant binds to Raf but not PI(3)K or RalGDS, the 12V,37G mutant binds to RalGDS but not Raf or PI(3)K, and the 12V,40C mutant binds to PI(3)K but not Raf or RalGDS (33, 39, 46, 47). The expression vector also contained the selectable marker gene neo; multiple G418-resistant colonies were selected, pooled, and tested for expression of Ras protein by Western blotting with anti-Ras antibody. As shown in Fig. 1A, endogenous levels of Ras protein were at the limit of detection in empty vector controls (designated neo), but all Ras mutants were expressed at similar, easily detectable levels.

View larger version (29K): [in this window] [in a new window]   FIG. 1.   Expression of H-Ras 12V and effector loop mutant proteins in NIH 3T3 and Rat 6 cells. (A) NIH 3T3 and Rat 6 cells were stably transfected with expression vectors for the indicated proteins. The identity of the more rapidly migrating band of variable intensity is unknown, but it is not endogenous Ras. (B) NIH 3T3 and Rat 6 cells were transfected with combinations of expression vectors for the indicated proteins. Cell extracts (100 µg) were analyzed by SDS-polyacrylamide gel electrophoresis and immunoblotting with anti-Ras antibodies. The higher signal strength of immunoreactive Ras proteins in panel B than in panel A is due to a longer film exposure in panel B. Quantitation of the respective Ras proteins by densitometry gave the following ratios (in parentheses): for NIH 3T3 cells, 12V (1.0), 12V,35S (2.0), 12V,37G (3.2), 12V,40C (2.1), 12V,35S plus 12V,37G (1.5), 12V,35S plus 12V,40C (2.0), 12V,37G plus 12V,40C (1.6); for Rat 6 cells, 12V (1.0), 12V,35S (1.0), 12V,37G (0.7), 12V,40C (1.0), 12V,35S plus 12V,37G (1.9), 12V,35S plus 12V,40C (1.1), 12V,37G plus 12V,40C (1.5). Note that the same 12V-expressing cells and the same amounts of extract were used for panels A and B.

View larger version (65K): [in this window] [in a new window]   FIG. 2.   Soft agar colony formation and morphology in adherent cultures of NIH 3T3 and Rat 6 cells that express H-Ras 12V and effector loop mutants. NIH 3T3 and Rat 6 cells that expressed neo or the indicated H-Ras constructs were cultured in soft agar or on tissue culture dishes (monolayer) as described in Materials and Methods and photographed. (A) Soft agar cultures. The photomicrographs demonstrate relative colony size; for colony-forming efficiencies, see Table 1. (B) Monolayer cultures of NIH 3T3 cells. Original magnification, ×40.

View larger version (44K): [in this window] [in a new window]   FIG. 3.   Analysis of Erk2 activity in NIH 3T3 and Rat 6 cells that express H-Ras 12V and various effector loop mutants. Cells expressing the indicated constructs were cultured in methylcellulose and harvested. Erk2 was immunoprecipitated and assessed for activity with myelin basic protein (MBP) as a substrate as described in Materials and Methods. Erk2 levels in each cell type were also assessed by Western blotting with anti-Erk2 antibodies. The numbers below the MBP kinase assay represent relative activities derived by densitometric analysis of the signal, with the neo lane designated 1.

Effects of various Ras mutants on adhesion-mediated expression and regulation of cell cycle proteins. Phosphorylation of pRB, cyclin E-dependent kinase activity, and expression of cyclin A are all fully dependent on cell anchorage in several different nontransformed cell types, including NIH 3T3 and Rat 6 (5, 10, 13, 16, 40, 51). Oncogenic Ras abrogates the adhesion dependence of these activities in both cell types, and its ability to do so is apparently linked to its ability to drive anchorage-independent growth (5, 16). We therefore asked to what extent, if any, expression of the Ras effector loop mutants might relieve adhesion dependence of these events. Because it is not possible to recover viable, nonadherent cells from soft agar cultures, we used a methylcellulose culture system that allows for nearly quantitative recovery of intact cells cultured under nonadherent conditions (16). It has been demonstrated previously that the growth properties in soft agar and methylcellulose of the fibroblast lines used in these studies are nearly identical (16, 49). Furthermore, the proliferative behavior of the effector loop mutant-expressing cells in methylcellulose culture was very similar to that in soft agar (data not shown).

View larger version (52K): [in this window] [in a new window]   FIG. 4.   Analysis of adhesion-regulated cell cycle activities in NIH 3T3 cells that express H-Ras 12V and effector loop mutants. Cells expressing neo or the indicated H-Ras constructs were grown on tissue culture plates (+ adhesion) or in preparative methylcellulose cultures ( adhesion) and were analyzed by immunoblotting or immunoprecipitation and kinase assay as follows. (A) pRB phosphorylation. Blots were probed with a specific anti-pRB antibody. The hyperphosphorylated (ppRB) and hypophosphorylated (pRB) forms are distinguished by their mobilities and are indicated by arrows. (B) Cyclin E-dependent kinase activity. Cyclin E was immunoprecipitated and analyzed for associated histone H1 kinase activity. The bottom panel shows a Western blot of cyclin E, demonstrating approximately equivalent levels of cyclin E in the different cell types under the various culture conditions. (C) Expression of cyclin A. Blots were probed with a specific anti-cyclin A antibody. Each experiment was performed at least twice on two independent extracts from each cell line. Representative data are shown. See Materials and Methods for additional details.

View larger version (46K): [in this window] [in a new window]   FIG. 5.   Analysis of adhesion-regulated cell cycle activities in Rat 6 cells that express H-Ras 12V and effector loop mutants. Cells expressing neo or the indicated H-Ras constructs were grown on tissue culture plates (+ adhesion) or in preparative methylcellulose cultures ( adhesion) and were analyzed by immunoblotting or immunoprecipitation and kinase assay as follows: pRB phosphorylation (A), cyclin E-dependent kinase activity (B), and expression of cyclin A (C). The more slowly migrating band in the minus adhesion lane of the 12V,40C-expressing cells is of unknown origin but has not been observed in other experiments and is not cyclin A. Details are as described in the legend to Fig. 4. Each experiment was performed at least twice on two independent extracts from each cell line. Representative data are shown.

View larger version (50K): [in this window] [in a new window]   FIG. 6.   Analysis of adhesion-regulated cell cycle activities in NIH 3T3 cells that express H-Ras 12V and various combinations of effector loop mutants. Cells expressing the indicated H-Ras constructs were grown on tissue culture plates (+ adhesion) or in preparative methylcellulose cultures ( adhesion) and were analyzed by immunoblotting or immunoprecipitation and kinase assay as follows: pRB phosphorylation (A), cyclin E-dependent kinase activity (B), and expression of cyclin A (C). See Fig. 3 for typical expression pattern of cyclin A in neo-expressing NIH 3T3 cells. Details are as described in the legend to Fig. 4. Each experiment was performed at least twice.

View larger version (55K): [in this window] [in a new window]   FIG. 7.   Analysis of adhesion-regulated cell cycle activities in Rat 6 cells that express H-Ras 12V and various combinations of effector loop mutants. Cells expressing the indicated H-Ras constructs were grown on tissue culture plates (+ adhesion) or in preparative methylcellulose cultures ( adhesion) and were analyzed by immunoblotting or immunoprecipitation and kinase assay as follows: pRB phosphorylation (A), cyclin E-dependent kinase activity (B), and expression of cyclin A (C). Details are as described in the legend to Figure 4. Each experiment was performed at least twice.

View larger version (40K): [in this window] [in a new window]   FIG. 8.   Effects of adhesion and various Ras proteins on expression of cyclin D1, p27kip1, and p21cip1 in NIH 3T3 cells. Cells expressing neo or the indicated H-Ras constructs were grown on tissue culture plates (+ adhesion) or in preparative methylcellulose cultures ( adhesion) and were analyzed by immunoblotting with specific antibodies to cyclin D1, p27kip1, or p21cip1, as indicated. Each experiment was performed twice on two independent extracts from each cell line. Representative data are shown.

Cooperation between Ras effector loop mutants and cyclins in inducing anchorage-independent growth. Expression of cyclins D1 and A is adhesion dependent in several cell types (13, 16, 51). Ectopic expression of each of these cyclins influenced adhesion-mediated regulation of cell cycle progression, although in most cases, it was not sufficient to induce colony formation in soft agar (16, 36, 51). The ras-mediated increase in cyclin D1 levels, observed in this and other studies (Fig. 8) (16 and 26), is thought to be important for transformation by this oncogene. Furthermore, ectopic expression of cyclin A rescued anchorage-independent growth of transformation-resistant ER-1-2/ras cells (16) (see the introduction). Thus, it was of interest to test whether ectopic expression of cyclin D1, E, or A could complement individual Ras effector loop mutants in the induction of anchorage-independent growth. NIH 3T3 and Rat 6 cells that expressed neo, 12V, or each of the three effector loop mutants were infected with recombinant retroviruses that drive expression of human cyclin D1, E, or A cDNA from the viral long terminal repeat (16). The parental vector, pBabePuro (31), also confers resistance to the drug puromycin. The cells were infected with one of the cyclin-expressing viruses or, as a control, a virus that lacked a cDNA insert. The infectants were selected in puromycin-containing medium, and puromycin-resistant colonies were then pooled and analyzed for expression of the various exogenous cyclins and colony formation in soft agar.

View larger version (65K): [in this window] [in a new window]   FIG. 9.   Analysis of ectopic expression of cyclins D1, E, and A in NIH 3T3 and Rat 6 cell derivatives. NIH 3T3 or Rat 6 cells that express neo, H-Ras 12V, or various effector loop mutants were infected with recombinant retroviruses that either lacked a cDNA insert () or harbored a human cyclin D1 (hCyclin D1), human cyclin E, or human cyclin A cDNA (+). Cell extracts were analyzed by immunoblotting with antibodies specific for cyclin D1, human cyclin E, or human cyclin A. Specific bands are indicated by the arrows. The human cyclin A band migrates between two nonspecific, cross-reactive bands and is also indicated by asterisks in the appropriate lanes.

	DISCUSSION

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Stable expression of oncogenic Ras in rodent fibroblast cell lines induces a wide array of responses referred to collectively as the transformed phenotype. Such responses are thought to be of relevance to the role of Ras in human cancers and include alterations in cell morphology, growth factor requirements, and gene expression. A response that shows an excellent correlation with tumorigenicity is anchorage-independent growth, measured as the ability to form colonies in semisolid medium (42). Recent studies have indicated that Ras functions by binding to multiple effector proteins that, in turn, activate distinct downstream signalling pathways (17, 28). A major task is to discern which effector pathways contribute to which aspects of the transformed phenotype. Ras effector loop mutants, which are defective for binding specific effector proteins while remaining competent for binding and activating others, are particularly well suited to such investigations (46). In this study, we have exploited Ras effector loop mutants to examine whether multiple Ras-regulated pathways are involved in the induction of anchorage-independent growth. It has previously been shown that several cell cycle events, including activation of G₁ Cdks and expression of cyclin A, are dependent on cell adhesion in nontransformed fibroblasts (5, 10, 13, 16, 40, 51) and that anchorage-independent growth most likely depends on the ability of Ras to supplant this requirement for adhesion (5, 16, 49). The effector loop mutants were therefore also used to assess whether multiple pathways might signal to the cell cycle machinery in cells cultured in suspension. We report here that although oncogenic Ras (12V) induced formation of colonies in soft agar by both NIH 3T3 and Rat 6 cells, each of three effector loop mutants (12V,35S, 12V,37G, and 12V,40C) had almost completely lost this ability. Pairwise combinations of these mutants, however, synergized to produce growth in soft agar by both cell types. The most likely explanation for these observations is that multiple Ras-mediated pathways are required for efficient induction of anchorage-independent growth. Synergy between specific effector loop mutants has also been observed previously in assays of focus formation and of induction of DNA synthesis following microinjection (15, 19, 39, 46). This report is, as far as we are aware, the first to demonstrate such synergy in stably transfected cell lines.

The ability of individual Ras effector loop mutants, and combinations of such mutants, to abrogate the adhesion dependence of specific cell cycle events is consistent with the conclusion drawn above. In Rat 6 cells, expression of individual mutants produced only trace levels of pRB phosphorylation and cyclin E-dependent kinase activity in the absence of cell-substratum adhesion. Furthermore, none led to expression of cyclin A under nonadherent conditions. In contrast, coexpression of 12V,35S plus 12V,37G or 12V,37G plus 12V,40C led to both colony formation in soft agar and loss of the anchorage requirement for these cell cycle activities. This correlation also held for the combination of 12V,35S and 12V,40C, which did not lead to significant levels of either colony formation or adhesion-independent expression of cyclin A in Rat 6 cells. These data suggest that an effector that binds to 12V,37G, but not the other two mutants, may be required for induction of anchorage-independent growth of this cell line. Two potential effectors that fit this description are RalGDS and Rin1 (14, 47).

NIH 3T3 cells responded to the effector loop mutants in a manner roughly similar to that observed with Rat 6 cells. The NIH 3T3 line was, however, different from Rat 6 in two important ways. First, in NIH 3T3 cells, all three mutants were partially able to drive pRB phosphorylation, cyclin E-dependent kinase activity, and expression of cyclin A in the absence of cell-substratum adhesion. This is striking and indicates that, at least in these cells, Ras may signal to the cell cycle machinery without binding to any two of the three effectors thus far implicated in transformation: Raf, PI(3)K, and RalGDS. It is not yet clear whether signalling by any of these pathways alone is sufficient to drive partial, anchorage-independent activation of the cell cycle machinery, because the effector mutants used in this study may bind to additional proteins that could also participate in these actions. For example, when assayed in the yeast two-hybrid system, 12V,37G bound to Rin1, and all three mutants bound to AF6 (14, 19).

NIH 3T3 cells that expressed 12V,37G or 12V,40C produced only trace levels of cyclin A in suspension, providing a reasonable explanation for their inability to form macroscopic colonies in soft agar (16, 49, 51). The 12V,35S-expressing cells, however, displayed higher levels of hyperphosphorylated pRB and cyclin A in suspension culture than did cells that expressed the other two mutants, yet they were equally impaired at forming colonies. The synergy in soft agar colony formation seen with NIH 3T3 cells that expressed combinations of mutants was, however, reflected in enhanced adhesion-independent expression of cyclin A by these cells. It is possible, therefore, that there is a tightly regulated threshold effect for cyclin A levels and transformation. Thus, the relatively small differences in cyclin A expression seen between NIH 3T3 cells that expressed 12V,35S and the effective paired combinations may have been sufficient to trigger an all-or-none response in macroscopic colony formation. Alternatively, there may be additional requirements for efficient induction of anchorage-independent growth that are synergistically regulated by the various effector loop mutants. Whether these putative requirements represent additional cell cycle events, or metabolic activities such as anaerobic glycolysis (41), is not known.

A second difference between NIH 3T3 and Rat 6 cells was that in the former cell line but not the latter, Ras effector loop mutants synergized with ectopic expression of various cyclins in the formation of colonies in soft agar. Cyclins D1, E, and A are each required for the G₁-to-S phase transition, and the levels of these proteins are rate limiting for G₁ phase progression (1, 12, 32, 36-38). Taken together, these data strongly suggest one important reason individual effector loop mutants are impaired at inducing anchorage-independent growth is that in the absence of adhesion-mediated signals, they are insufficient to activate fully the cell cycle machinery that controls G₁ phase progression and the G₁-to-S phase transition. Overall, cyclin A was much more effective at synergizing with the effector loop mutants than were cyclins D1 and E. This is consistent with our previous conclusion that the ability of ras to drive expression of cyclin A in the absence of cell adhesion is likely to be critical to the induction of anchorage-independent growth by this oncogene (16, 49). The failure of ectopic cyclin expression to complement individual effector loop mutants in Rat 6 cells may be due to the much weaker ability of these mutants to supplant the adhesion requirements of all of the cell cycle activities investigated in these cells relative to NIH 3T3 cells.

The conclusions drawn in this report are consistent with those derived from most other recent studies; i.e., multiple pathways contribute to, and may be required for, full transformation by Ras (15, 19, 20, 33, 39, 46, 47). There are, however, some exceptions worth mentioning. Khosravi-Far et al. reported that stable expression of the 12V,35S, 12V,37G, and 12V,40C mutants each led to growth in soft agar and tumorigenicity (19). These results were obtained with one strain of NIH 3T3 cells but not, apparently, with a second strain (19, 46). In another study Stang et al. investigated an exhaustive panel of effector loop mutants and concluded that the Raf pathway alone may be sufficient for transformation of Rat 2 cells (43). This may be due to a particular sensitivity of this cell line to this pathway or, possibly, to the fact that transformation was scored only by examination of the morphology of cells in drug-resistant colonies and not by more stringent assays such as growth in soft agar. The failure of the 12V,40C mutant to induce morphological transformation of NIH 3T3 or Rat 6 cells is also noteworthy. Microinjection of this construct into several different cell lines caused rearrangement of cortical actin and membrane ruffling to a degree similar to that observed with 12V alone (15, 39). This finding suggests that the requirements for stable morphological transformation and for the cytoskeletal alterations measured in these short-term assays may not be identical.

Taken together, the results of this study are most consistent with the interpretation that individual Ras effector pathways do not control individual aspects of the transformed phenotype; rather, multiple effector pathways are required for each aspect of the transformed phenotype. With regard to anchorage-independent growth, these pathways collaborate to supplant the adhesion requirements of specific cell cycle events which presumably drive cell proliferation in the absence of cell-substratum adhesion. It is hoped that the cell lines developed in this study will facilitate identification of the Ras-mediated pathways involved in anchorage-independent growth and the biochemical mechanisms by which they connect to the cell cycle machinery.