INTRODUCTION

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Steroid hormone receptors are members of a superfamily of ligand-dependent transcriptional activators which direct the expression of specific gene networks involved in regulating the differentiation and growth of reproductive tissues, as well as other metabolic processes. Receptors for steroid hormones are a subgroup of the nuclear receptor supergene family which have distinctive properties. In the absence of hormone, these receptors associate with heat shock proteins (HSPs) that serve as protein folding chaperones to maintain an active receptor conformation capable of receiving and responding to the hormonal signal (55). Steroid binding induces a series of changes in the receptor that leads to transcriptional activation, including a conformational change, dissociation from the oligomeric HSP complex, dimerization, and binding to hormone response elements (HREs) of target genes (see review in reference 62). Consensus HREs for steroid receptors are inverted palindromes arranged as hexanucleotide core motifs separated by 3 bp of undesignated sequence (61, 75). Steroid receptors bind preferentially as homodimers to HREs with the axis of dyad symmetry over the center of the palindromic element (19). Other members of the superfamily include receptors for nonsteroidal ligands, such as thyroid hormone receptor (TR), retinoic acid receptor (RAR), and vitamin D receptor (VDR) (35, 36). These nuclear receptors are distinguished from steroid receptors by the lack of stable interaction with HSPs (55), recognition of HREs that are arranged as direct repeats (DR) with variable half-site spacing, and binding to DR elements as heterodimers with retinoid X receptor (RXR) as the common dimer partner (29, 34, 63, 72 74).

How receptor binding to HREs enhances transcription of target genes is not well understood. Nuclear receptors are thought to stabilize the formation of a preinitiation complex at promoters through protein-protein interactions with basal transcription factors (23, 59), TATA-binding protein-associated factors (24), or a specific group of coactivators that are thought to provide a bridge between the receptor and the basal transcriptional machinery (22, 25, 26, 43, 59, 66).

Receptor interacting proteins which can either facilitate or inhibit receptor binding to target DNA sequences have also been described. A thyroid hormone receptor uncoupling protein (TRUP) was isolated that interacts with TR and RAR to block DNA binding in vitro and transactivation function within the cell (6). The calcium binding protein, calreticulin, has been reported to inhibit the binding of several different nuclear receptors to their target DNA sites in vitro as a result of interacting with conserved sequences in the DNA binding domains (DBDs) of all nuclear receptors. When overexpressed in mammalian cells, calreticulin also can inhibit receptor-dependent transcription (5, 12, 13). Additionally, several studies have reported the existence of proteins that stimulate sequence-specific DNA binding of nuclear receptors. Purification of recombinant receptors typically results in a loss of DNA binding activity that can be partially or fully restored by the addition of other protein(s). It has been well established that RXR enhances the DNA binding and transcriptional activity of a subgroup of nuclear receptors (TR, VDR, and RAR) by heterodimerization, where RXR directly contacts HREs (29, 34, 35, 72, 74). RXR has not been observed to functionally interact with any of the steroid hormone receptors. Studies have also described proteins that facilitate the binding of steroid receptor homodimers to palindromic HREs in vitro. For the most part, the protein(s) responsible for facilitating steroid receptor-DNA binding has not been identified and has been characterized as an enriched protein fraction or as an isolated protein of a specific molecular mass (9, 11, 14, 30, 37, 41, 51, 56). We previously showed that mammalian-cell nuclear extracts contain a protein(s) that substantially increases the binding of human progesterone receptor (PR) to its target DNA in vitro (15). The major nuclear factor responsible for enhancing PR-DNA binding was later identified as the chromatin nonhistone high-mobility group protein 1 (HMG-1) (42, 46). Whether HMG-1 or other accessory proteins that enhance steroid receptor-DNA binding in vitro have a physiological role in steroid receptor action in the cell has not been determined.

HMG-1 and the closely related HMG-2, referred to collectively as HMG-1/-2, are modular proteins that contain duplicated amino-terminal and centrally located DNA binding domains, termed HMG boxes, and an acidic carboxyl terminus. HMG-box motifs have been identified in several other proteins, some of which are sequence-specific transcription factors (7, 8, 31, 32). HMG-1/-2 have no known specific DNA recognition sequence; they prefer to bind to specific DNA structures, such as prebent DNA or the sharp angles at four-way junction DNA (7). HMG-1/-2 also have the ability to induce bends in DNA, and they bind in the minor groove. Based on nuclear magnetic resonance (NMR) solution structure, the tertiary structure of the HMG box from different proteins has been reported to be composed of three  helices folded into an L shape configuration believed to be important for the characteristic ability of HMG-box proteins to recognize and bind to bends or other distortions in DNA structure (49, 68). Although the physiological function of HMG-1/-2 remains poorly understood, this ability to recognize and manipulate DNA structure has led to the idea that these proteins function as architectural factors in processes that require transient manipulation of DNA structure, such as DNA repair, recombination, replication, and transcription (20, 70). As evidence that they can function as architectural transcriptional cofactors, HMG-1/-2 have been reported to facilitate the binding of several types of sequence-specific transcription factors to their target DNA sites; these factors include the octamer transcription factors Oct-1, Oct-2, and Oct-6 (77); the homeotic protein HOXD9 (73); the adenovirus major late promoter transcription factor MLTF (60, 67); p53 (25); and progesterone receptor (39, 42). In addition, transient-cotransfection assays demonstrated that HMG-1/-2 enhanced the transcriptional activity of the octamer transcription factors and HOX proteins within mammalian cells (73, 77). In a cell-free transcription assay with purified components, HMG-1/-2 were also found to be essential for activator-mediated assembly of the TFIID-TFIIA initiation complex on several promoters (54). Genetic evidence for a functional role of HMG-1/-2 in transcriptional regulation was recently provided by studies that deleted the HMG-like NHP6A and NHP6B genes in the yeast Saccharomyces cerevisiae (44). Mutant yeast cells carrying disruptions for both NHP6A and NHP6B exhibited a substantial reduction in activated transcription of a subset of yeast genes without altering basal promoter activity.

In the present study we investigated whether HMG-1 enhancement of the sequence-specific DNA activity of PR occurs with other members of the nuclear receptor superfamily. By use of cotransfection assays, we also investigated the effect of HMG-1 on the transcriptional activity of nuclear receptors within mammalian cells. We show that HMG-1 and the closely related HMG-2 increase the sequence-specific DNA binding activity in vitro and the transcriptional activity in whole cells of all of the steroid receptors tested. In contrast, HMG-1/-2 had little or no effect on the in vitro DNA binding activity of the nonsteroid class of nuclear receptors tested, including VDR, RXR, and RAR, and had minimal effect on VDR-mediated gene transcription in vivo. These results suggest that HMG-1/-2 are previously unrecognized positive coregulatory proteins for the steroid hormone subgroup of the nuclear receptor superfamily.

	MATERIALS AND METHODS

Top Abstract Introduction Materials & Methods Results Discussion References

Materials. Promegestone (17,21-dimethyl-19-norpregna-4,9-dien-3,20-dione) was obtained from NEN Life Sciences (Boston, Mass.). Other steroids (triamcinolone acetonide, 17-estradiol, and dihydrotestosterone) were purchased from Sigma Chemical Co. (St. Louis, Mo.). 1,25-Dihydroxy-vitamin D₃ [1,25-(OH)₂D₃] was obtained from Solvay Duphar and 9-cis-retinoic acid was purchased from Sigma. [³H]sodium acetate was from ICN Pharmaceuticals (Irvine, Calif.); coenzyme A and acetyl coenzyme A synthase were obtained from Sigma. Talon metal ion affinity resins were purchased from Clontech (Palo Alto, Calif.), and nickel chelation affinity resins were obtained from Qiagen (Chatsworth, Calif.). The radionucleotides [-³²P]dATP (3,000 Ci/mmol) and [-³²P]dCTP (3,000 Ci/mmol) were purchased from NEN Life Sciences. The following mouse monoclonal antibodies (MAbs) to steroid receptors were used: AB-52 prepared against human PR, which recognizes both the A and B receptor forms (16); h151 raised against a synthetic peptide corresponding to amino acids 287 to 300 of the hinge region of human estrogen receptor (ER) (17); and N441 raised against a synthetic peptide corresponding to amino acids 340 to 356 in the amino-terminal region of the human androgen receptor (AR; unpublished data). Polyclonal rabbit antiserum raised against human glucocorticoid receptor (GR) was provided by John Cidlowski (National Institute of Environmental Health Sciences, Research Triangle Park, N.C.). A rat MAb to VDR, 9A7, was provided by Wesley Pike (University of Cincinnati, Cincinnati, Ohio). Polyclonal antisera against human RXR and RAR have been previously described (58). A mouse immunoglobulin M (IgM) MAb (clone 854/E10) to calf thymus HMG-1 was prepared as described previously (47), and a rabbit polyclonal antibody specific for HMG-2 was provided by Raymond Reeves (Washington State University, Pullman, Wash.).

Expression of recombinant receptors and HMG-1 in the baculovirus system. Recombinant baculovirus transfer vectors for human PR-A and PR-B were constructed by inserting the cDNAs for each receptor isoform into BamHI sites of pBlueBacHis-2 (InvitroGen, San Diego, Calif.). This placed PR coding sequences in frame with amino-terminal plasmid sequences that contain an ATG translation start site, six sequential histidine residues, and an enterokinase cleavage site. A polyhistidine-tagged human GR baculovirus transfer vector was constructed by inserting a BamHI-XhoI fragment from pI9 (18) encoding amino acids 9 to 777 of human GR in frame into pBlueBacHis-2 (C) restricted with BamHI and HindIII. The XhoI and HindIII ends of GR cDNA were blunted by filling in before joining. The pI9 plasmid was provided by Ron Evans (Salk Institute, San Diego, Calif.). A 2.8-kb full-length human androgen receptor (hAR) cDNA, plus sequences coding for one extra amino-terminal alanine and six histidine residues, was cloned into a 9.1-kb pAcC4 baculovirus transfer vector (Cetus Corp.) that resulted in the following amino-terminal sequence: Met-Ala-His₆-Glu-Val. The hAR recombinant baculovirus transfer vector was constructed by PCR amplification with a primer that placed an NcoI site at the starting methionine of human AR, making it coincident with the position of the starting methionine of the polyhedron protein. A triple-ligation reaction was performed as previously described (71). A recombinant baculovirus transfer vector for HMG-1 was constructed by inserting rat HMG cDNA from pBS-rHMG-1 into EcoRI and SalI sites of pBlueBacHis-2(B). The rat HMG coding region was placed in frame with amino-terminal sequences of the plasmid containing an ATG translation start site, six sequential histidine residues, and an enterokinase cleavage site. A recombinant baculovirus transfer vector for steroid receptor coactivator 1 (SRC-1) (43) was constructed by inserting SRC-1 cDNA (amino acids 1 to 1140) from pBK-CMVSRC-1 (provided by Bert O'Malley, Ming-Jer Tsai, and Sergio Oñate, Baylor College of Medicine) into BamHI and PstI sites of the baculovirus transfer plasmid, pBlueBacHis-2(C). The SRC-1 coding region was also inserted in frame with amino-terminal plasmid sequences containing an ATG translation start site, six sequential histidine residues, and an enterokinase cleavage site. Cloning junctures in all transfer plasmids were sequenced (Sequenase 2.0; U.S. Biochemicals), and inserts were determined to be correctly oriented and in frame with the starting ATG codon and the six sequential histidine residues.

Expression of recombinant VDR, RXR, and RAR in yeast. Human VDR, RXR, and RAR were expressed as polyhistidine-tagged proteins in the protease-deficient yeast strain BJ2168 from a copper-inducible promoter on 2µm plasmids as described previously (3). Briefly, transformed yeast were grown in 2-liter volumes to an A₆₀₀ of 1.0 in minimal selection medium, and expression was stimulated by addition of 100 µM copper sulfate for 16 h at 30°C. VDR cultures were also induced by addition of 1 µM 1,25-(OH)₂ D₃. Cells were then harvested and lysed with acid-washed glass beads in the following buffer: 10 mM sodium phosphate, pH 8.0; 0.4 M KCl; 0.5 mM phenylmethylsulfonyl fluoride (PMSF); aprotinin, 1 µg/ml; and 5 mM -mercaptoethanol. Lysates were centrifuged at 100,000 × g for 45 min at 4°C to yield a soluble supernatant.

Purification of polyhistidine-tagged nuclear receptors with metal ion affinity columns. For polyhistidine-tagged steroid receptors expressed from baculovirus vectors, Sf9 insect cells were lysed in the following buffer: 20 mM Tris-HCl, pH 8.0; 350 mM NaCl; 10 mM imidazole; 5% glycerol; and a cocktail of protease inhibitors (16). All procedures were done at 0 to 4°C. Cell lysates were centrifuged at 100,000 × g for 30 min, and the supernatant was taken as a soluble whole-cell extract. Whole-cell extract was passed once over Talon resins in a column at a flow rate of 1 to 2 ml/min. The resins were washed with cell lysis buffer until the optical density at 280 nm returned to the buffer baseline. Bound receptors were eluted under nondenaturing conditions by competition with 100 mM imidazole. Receptors were eluted into siliconized tubes to prevent binding to surfaces, and dithiothreitol (DTT; 1 mM), zinc chloride (1 µM), and MgCl₂ (1 mM) were added immediately to stabilize receptor DNA and steroid binding activity. Samples were stored at 80°C in aliquots and remained stable for DNA binding activity for several months if not repeatedly frozen and thawed.

Purification of HMG-1 and HMG-2. A non-acid extraction method was used to purify HMG-1 and HMG-2 from calf thymus by a modification of the method originally defined by Adachi et al. (1). Fresh calf thymus (60 g) was cut into small pieces, trimmed of connective tissue, and homogenized in a stainless-steel blender in 6 volumes (tissues to buffer) of 10 mM sodium citrate buffer containing 0.15 M NaCl and 1 mM PMSF. The homogenate was filtered through cheesecloth and centrifuged for 15 min at 2,000 × g. The pellet was then washed three times by resuspension in homogenization buffer and centrifugation at 2,000 × g. The pellet was resuspended in 180 ml of 50 mM Tris-HCl (pH 7.8) containing 3 mM PMSF and then centrifuged for 10 min at 6,000 × g. This step was repeated. The resulting pellet containing nuclei and other membrane fractions was resuspended in a buffer (10 mM Tris-HCl, pH 7.8; 0.35 M NaCl; 5 mM DTT; 1 mM PMSF) in a glass-glass Dounce homogenizer. After homogenization, samples were left on ice for 1 h with brief intermittent vortexing to release 0.35 M NaCl-extractable nuclear proteins. The samples were then centrifuged at 5,000 × g for 20 min. The salt extraction of the nuclear pellet was repeated, and the two salt nuclear extracts were combined and centrifuged at 50,000 × g for 30 min. The supernatant was dialyzed overnight at 4°C against 10 mM Tris-HCl (pH 7.8) containing 1 mM DTT. The dialyzed salt nuclear extract was then passed over a PBE94 chromatofocusing column with a peristaltic pump at a flow rate of 1 to 2 ml/min. The column was washed and eluted with a linear 0 to 1.5 M NaCl gradient (in 10 mM Tris-HCl, pH 7.8; 5 mM DTT), and 1.0-ml fractions were collected and analyzed by sodium dodecyl sulfate (SDS)-12% polyacrylamide gel electrophoresis (PAGE) and Coomassie blue staining for the presence of HMG-1/-2. The peak fractions containing HMG-1/-2 were pooled, dialyzed against 10 mM Tris-HCl (pH 7.8)-1 mM DTT, and rechromatographed on a second PBE94 column. The fractions containing HMG-1 and HMG-2 were pooled and stored at 80°C.

Coimmunoprecipitation assay. Purified calf thymus HMG-2 (1,000 ng) was incubated with purified PR-B (200 ng) for 1 h at 4°C. The samples were then incubated for 3 h at 4°C on an end-over-end rotator with a 100-µl suspension of protein A-Sepharose that was prebound with a rabbit polyclonal antibody (2 µg) to HMG-2. As controls to determine the extent of nonspecific binding, blank protein A-Sepharose-coated beads and anti-HMG-2 antibody-coated beads were incubated with PR-B (200 ng) in the absence of HMG-2. Beads were washed four times with TEDG_N100 (10 mM Tris base, pH 7.4; 1 mM EDTA; 1 mM DTT; 100 mM NaCl; 10% glycerol), transferred to a new tube, and washed twice more. Bound PR-B was eluted with SDS-sample buffer and analyzed by Western blotting with the PR-specific MAb 1294. The same coimmunoprecipitation assay was performed except that 200 ng of purified human VDR was used in place of PR-B and protein A-Sepharose eluates were analyzed for VDR by Western blotting with a rat MAb (9A7) to human VDR.

Immobilized metal affinity chromatography pull-down assays. Baculovirus-expressed polyhistidine-tagged HMG-1 (10 µg) was incubated with non-histidine-tagged PR-B (8 µg) that was expressed in baculovirus and purified by MAb affinity chromatography as described in earlier studies (42, 46). Samples were incubated for 30 min at 4°C and then added to Talon resins in suspension and incubated for another 1 h at 4°C. Talon resins were washed four times in 20 mM Tris-HCl (pH 8.0)-10% glycerol-100 mM NaCl-15 mM imidazole, transferred to a new tube, and washed twice more. Bound proteins were eluted with SDS-sample buffer, and PR-B was detected by Western blotting with the PR-specific MAb 1294.

EMSA. DNA binding was determined by electrophoretic mobility shift assay (EMSA) for PR and AR under the same conditions as described previously (42, 46, 47). Briefly, receptors in whole-cell or purified extracts of Sf9 cells (amounts per assay are indicated in the figure legends) were incubated for 1 h at 0 to 4°C with 0.3 ng of a ³²P-end-labeled glucocorticoid response element-progesterone response element (GRE-PRE) oligonucleotide (specific activity, 100,000 to 300,000 cpm/ng) in a DNA binding buffer containing 10 mM Tris-base (pH 7.5), 50 mM NaCl, 5 mM DTT, 2 mM MgCl₂, 1 mM EDTA, and 5% glycerol. The oligonucleotide was end labeled by filling in of 5' single-stranded ends with the Klenow fragment of DNA polymerase by using [-³²P]dATP and [-³²P]dCTP. The binding reactions also contained 1 µg of gelatin (or albumin) as a carrier protein and 80 to 100 ng of competitor poly(dA-dT)-poly(dA-dT). All components of the binding reaction were preincubated for 30 min at 4°C prior to addition of the [³²P]DNA probe. After 1 h, DNA binding reactions (25 µl) were electrophoresed on 5% polyacrylamide (40:1 acrylamide/bisacrylamide ratio) gels in 0.5× TAE buffer (0.02 M Tris-acetate, pH 8.0; 0.5 mM EDTA) with cooling to maintain the gel temperature at 4°C. Gels were dried under vacuum and autoradiographed, and free [³²P]DNA and [³²P]DNA complexes were quantitated by direct scanning of the gels for radioactivity by a series 400 Molecular Dynamics PhosphorImager. DNA binding as determined by EMSA for other nuclear receptors was accomplished by similar methods with the following minor modifications. A different binding buffer that included 10 mM HEPES (pH 7.8), 50 mM KCl, 4 mM MgCl₂, and 12% glycerol was used with GR, ER, VDR, RAR, and RXR. Additionally, 2.5% glycerol was added to the polyacrylamide gel, and the electrophoresis buffer was 0.25× TBE (0.02 M Tris-borate, pH 8.0; 0.02 M boric acid; 0.5 M EDTA).

SDS-PAGE and Western blots. Receptors or HMG-1/-2 were subjected to SDS-PAGE on 12, 10, or 7.5% polyacrylamide gels as previously described (42, 46, 47), and protein bands were detected by staining with Coomassie blue or silver (21). Separated proteins were transferred to nitrocellulose membranes and incubated with the appropriate receptor-specific antibody overnight at 4°C. For mouse MAbs, Western blot detection was done with a secondary goat anti-mouse-horseradish peroxidase colorimetric method or by using rabbit anti-mouse IgG and ³⁵S-labeled protein A followed by autoradiography. With rabbit polyclonal antibodies, goat anti-rabbit-horseradish peroxidase (Cappel) was used for detection. For the rat 9A7 MAb to human VDR, the detection method was with rabbit anti-rat IgG followed by the addition of S-labeled protein A and autoradiography.

Expression plasmids and reporter gene constructs. The expression plasmids pHMG-1 and pHMG-2 contain genomic clones of mouse HMG-1 and HMG-2, respectively, under the control of their own constitutive promoters that were then cloned into the EcoRI site of pBluescript KS(+) as previously described (73). pBluescript KS(+) from Stratagene was used as an empty vector control. A mammalian-cell expression plasmid for human PR-B (pPR-B) was provided by Donald McDonnell (Duke University, Durham, N.C.) (64), an expression plasmid for human AR (p5HBL-AR-A) was provided by Elizabeth Wilson (University of North Carolina, Chapel Hill, N.C.), and an expression plasmid for GR (pSVGR) was provided by Ron Evans (Salk Institute). Human VDR expression plasmid (pRShVDR) for mammalian cells was under the control of the Rous sarcoma virus promoter (76).

Cell culture and transient transfections. COS1 cells were maintained in Dulbecco modified Eagle medium (DMEM; Gibco-BRL) supplemented with 10% fetal bovine serum (HyClone) and were plated in multiwell dishes (six-well dishes; Falcon Plastics) at a density of 175,000 to 200,000 cells/well. HeLa cells were plated at the same density in DMEM supplemented with 5% fetal bovine serum. Cells were transiently transfected by an adenovirus-mediated method described previously (4). Purified defective adenovirus particles, covalently coupled to poly-L-lysine (provided by Nancy Weigel, Baylor College of Medicine, Houston, Tex.), were mixed with plasmid DNA for 30 min at room temperature in 20 mM HEPES buffer (pH 7.8) followed by the addition of poly-L-lysine at 2.4 µg/ml for an additional 30 min at room temperature. Just prior to transfection, culture medium was removed from the wells and replaced with 3 ml of serum-free DMEM per well, and the adenovirus-plasmid mixture was added directly to the medium at a multiplicity of infection of 250 to 400 viral particles/cell. Cells were then incubated for 2 h at 37°C, and then 3 ml of DMEM supplemented with 10% fetal bovine serum was added per well to bring the final serum concentration to 5%. The cells were then incubated for another 24 h at 37°C. After 24 h the cells were treated for another 24 h with and without hormone. At 48 h after transfection, cell monolayers were rinsed with CAT-LUC wash buffer (40 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM EDTA), and the cells were lysed in the well by the addition of 300 µl of cell lysis buffer (20 mM potassium phosphate, pH 7.4; 5 mM MgCl₂; 0.5% Triton X-100) per well; the lysates were then measured for CAT or LUC activity. To estimate the percentage of transfected cells, parallel cells in six-well dishes were transfected with a cytomegalovirus (CMV)--galactosidase (-Gal) reporter gene. At 48 h after transfection, cells were fixed in the well with 0.5% glutaraldehyde in phosphate-buffered saline and incubated with a 2% X-Gal (5-bromo-4-chloro-3-indolyl--D-galactopyranoside) staining solution (4). The percentage of blue-stained cells averaged 15% for HeLa cells and 30% for COS1 cells. To control for variation in transfection efficiency, the cells were routinely cotransfected with a CMV--Gal internal control plasmid (2 ng/well). Cotransfection cultures with receptors and HMG-1/-2 expression plasmids also contained appropriate amounts of empty control vectors so that all cultures received the same amount of total plasmid DNA.

CAT and LUC assays. CAT enzyme activity was measured by a radiometric phase-extraction method as previously described (38). Enzyme activity was calculated as the counts per minute of ³H-labeled acetyl coenzyme A converted per µg of protein in the cell lysate. LUC assays were done with a Monolight luminometer 2001 or 2010. Aliquots of cell lysates (5 to 50 µl) were added to 0.35 ml of LUC assay buffer (100 mM potassium phosphate, pH 7.8; 15 mM magnesium sulfate, 5 mM ATP; 1 mM DTT), and light output was measured for 10 s with a built-in 2-s delay after injection of 100 µl of 1 mM luciferin. The protein concentration was measured by Bradford assay, and equal amounts of protein (10 to 30 µg) were added to the CAT assays. Specific CAT and LUC activities were determined by subtraction of the assay background obtained from lysates of mock-transfected cells that received no plasmids. -Gal activity from internal CMV--Gal control plasmids was measured in a luminometer by a chemiluminescent method according to the manufacturer's instructions (Tropix, Inc., Bedford, Mass.), and CAT and LUC activities were corrected for variations in -Gal activity. Cell transfections were all done in duplicate culture wells, and reporter gene assays were performed in duplicate with each lysate. Therefore, all CAT and LUC values are averages from four assay determinations.

	RESULTS

Top Abstract Introduction Materials & Methods Results Discussion References

Purification of recombinant nuclear receptors and HMG-1/-2 proteins for in vitro DNA binding studies. Because our previous studies dealt only with PR (42, 46), it was used in this study as a standard for comparison. Human PR is expressed as two isoforms: an amino-terminal truncated PR-A and a full-length PR-B. The two PR isoforms are identical in their centrally located DNA binding domains and carboxyl-terminal ligand-binding domains (28). PR-A and PR-B have indistinguishable DNA binding properties in vitro (10), and HMG-1 was previously observed to stimulate the binding of both PR-A and PR-B to target DNA sites in a similar manner (42, 46). All DNA binding results in this study are shown with PR-A; indistinguishable results were obtained with PR-B (data not shown). In order to have a common purification procedure, all nuclear receptors in this study were expressed as recombinant proteins with an amino-terminal polyhistidine tag for use in metal ion affinity chromatography under nondenaturing conditions. The exception was human ER, which was expressed as a nonfusion protein and purified by using sequence-specific DNA affinity columns as previously described (39). Steroid receptors, including human PR, GR, AR, and ER, were expressed in Sf9 insect cells by using the baculovirus system and were bound to their cognate hormonal ligands during expression. Nonsteroid nuclear receptors, including human VDR, RAR, and RXR, were expressed in yeast cells.

View larger version (34K): [in this window] [in a new window]   FIG. 1.   Purification of baculovirus-expressed steroid receptors. Human PR-A, AR, and GR were expressed as polyhistidine-tagged proteins in baculovirus and were purified by metal ion affinity resins (Talon). Human ER was expressed as a nonfusion protein in baculovirus and purified by using a DNA affinity column. (A) (Left panel) Silver-stained SDS-7.5% PAGE of PR-A purification fractions. Lanes: 1, Sf9 whole-cell extract (WCE; 5 µl); 2, Talon resin flowthrough (F/T; 5 µl); 3, column wash (Wash; 50 µl); and 4, 100 mM imidazole (Idz) eluate (10 µl). (Right panel) Western blot of the same PR-A purification fractions (5 µl) with the AB-52 MAb. (B) (Left panel) Silver-stained SDS-PAGE of purified AR and GR eluted from Talon resins by 100 mM imidazole. ER is an NaCl eluate of a DNA affinity resin. (Right panel) Western blots of purified AR, GR, and ER were done with the following receptor specific antibodies: N441, an MAb to AR; a polyclonal antibody to GR; and h151, an MAb to human ER.

View larger version (34K): [in this window] [in a new window]   FIG. 2.   Purification of nonsteroid nuclear receptors. Human RXR, RAR, and VDR were expressed in yeast cells as polyhistidine-tagged proteins and were purified by using metal ion affinity resins. Purified products were analyzed by SDS-PAGE and silver staining (left panel) and by Western blotting with receptor-specific antibodies (right panel).

View larger version (43K): [in this window] [in a new window]   View larger version (72K): [in this window] [in a new window]   View larger version (73K): [in this window] [in a new window]   FIG. 3.   HMG-1 and HMG-2 purification and effects on PR-DNA binding in vitro. (A) HMG-1 and HMG-2 from calf thymus were purified and separated on a PBE94 chromatofocusing column as described in Materials and Methods. Coomassie blue-stained SDS-polyacrylamide gels (12%) and Western blot analysis of purified HMG-1/-2 fractions were prepared as follows: lane 1 (pool 1), HMG-2; lane 2 (pool 2), HMG-1 and HMG-2; lane 3 (pool 3), HMG-1; lane 4, purified recombinant polyhistidine-tagged HMG-1. Western blots were done with an IgM MAb (854/E10) that recognizes both HMG-1 and HMG-2. (B and C) The influence of purified calf thymus HMG-1 (pool 3) and HMG-2 (pool 1) on the binding of purified PR-A to a 32-bp PRE oligonucleotide probe as assessed by EMSA. A constant amount (30 nM) of purified PR-A was incubated with increasing amounts of purified HMG-1 (B) or HMG-2 (C). The amounts of HMG-1 and HMG-2 used were 30, 50, 70, 100, 200, 300, 400, 500, 700, and 1,000 µg/assay in lanes 3 to 12, respectively.

HMG-1 and the closely related HMG-2 facilitate the sequence-specific DNA-binding activity of recombinant purified human PR. Our previous studies showing that HMG-1 increased the DNA binding activity of PR were done with a nonfusion baculovirus-expressed PR purified by MAb affinity chromatography (42, 46). Therefore, before analyzing the effects of HMG-1 on other nuclear receptors, we determined whether polyhistidine-tagged PR purified by metal affinity resins would respond in a similar manner to HMG-1 as had antibody-purified PR. Crude and highly purified preparations of baculovirus expressing polyhistidine-tagged PR-A (Fig. 1A) were analyzed by EMSA for binding to a PRE oligonucleotide. As with our results with nonfusion MAb-purified PR (42, 46), polyhistidine-tagged PR-A in crude extracts of Sf9 insect cells bound to the PRE probe, whereas an equal amount of purified polyhistidine-tagged receptor exhibited no DNA binding activity (Fig. 4A). The addition of calf thymus nuclear extracts or purified calf thymus HMG-1 stimulated the formation of PR-DNA complexes (Fig. 4A). As controls for the general effect of other proteins on the stability of purified PR, the addition of unrelated proteins such as albumin or insulin had no effect on PR-DNA binding (Fig. 4A). The DNA complex stimulated by HMG-1 is specific, as demonstrated by supershifts with a MAb to PR and by competition with unlabeled homologous PRE oligonucleotide but not by an ERE oligonucleotide (Fig. 4A). Purified recombinant baculovirus-expressed HMG-1 exhibited stimulatory activity similar to that of native HMG-1 purified from calf thymus, indicating that minor contaminants in calf thymus preparations are not responsible for stimulating PR-DNA binding activity (data not shown). In previous studies we observed that bacterially expressed and purified HMG-1 stimulated PR-DNA binding (42). The fact that one source of purified cellular HMG-1 and two sources of purified recombinant HMG-1 all have similar activities strongly indicates that the stimulation of PR-DNA binding is an intrinsic property of HMG-1/-2 and is not due to contaminating proteins.

View larger version (66K): [in this window] [in a new window]   FIG. 4.   HMG-1/-2 stimulate the sequence-specific DNA binding activity of all steroid receptors tested. (A) Equal amounts (30 nM) of polyhistidine-tagged PR-A in Sf9 whole-cell extract (WCE) or affinity-purified PR-A were analyzed for binding to a 32P-labeled palindromic GRE-PRE oligonucleotide by EMSA. Lanes contain Sf9 whole-cell extract (WCE), purified PR alone (), or purified PR plus 1 µg of ovalbumin (+Oval), 1 µg of nuclear extract (+NE) from calf thymus, 1 µg of insulin (+INS), or 300 ng of purified calf thymus HMG-1 (+HMG-1). The HMG-1-stimulated PR-DNA complexes were supershifted by the PR-specific MAb, AB-52, and were competed by a 100-fold molar excess of the PRE oligonucleotide (100×sp) but not by an ERE oligonucleotide (100×ns). (B) Equal amounts (70 nM) of baculovirus-expressed AR in WCE and affinity-purified AR were analyzed for binding to the GRE-PRE oligonucleotide by EMSA as described for panel A. (C) Equal amounts (10 nM) of baculovirus-expressed ER in WCE and affinity-purified ER were analyzed for binding to an ERE oligonucleotide by EMSA as described for panel A with or without the addition of 300 ng of purified calf thymus HMG-1. (D) Equal amounts (200 nM) of baculovirus-expressed GR in WCE and affinity-purified GR were analyzed for binding to the GRE-PRE by EMSA as described for panel A. All receptors were bound to their cognate (see Materials and Methods) hormonal ligands in Sf9 cells during expression.

HMG-1/-2 facilitate the sequence-specific DNA binding activity of all steroid receptors tested. We next analyzed the influence of HMG-1/-2 on the DNA binding activities of other steroid receptors. Because PR, GR, and AR all recognize a common GRE, these receptors were assayed for binding to the same palindromic GRE oligonucleotide probe. ER binding was assessed with a palindromic ERE probe. As with the results with PR, recombinant baculovirus-expressed AR, GR, and ER in crude Sf9 cell extracts bound specifically to their target DNA probes. Equal amounts of purified preparations of these same receptors exhibited a loss of DNA binding activity that was restored by the addition of either HMG-1 or HMG-2 but not by the addition of other unrelated proteins (Fig. 4B to D). The DNA complexes stimulated by HMG-1 or HMG-2 were specific, as demonstrated by supershifts with the appropriate receptor-specific antibody and by competition with homologous unlabeled DNA probes (Fig. 4B to D). Similar to results with PR, HMG-1 and HMG-2 were found to be interchangeable with respect to stimulating the DNA binding activity of other purified steroid receptors (data not shown).

View larger version (21K): [in this window] [in a new window]   FIG. 5.   Saturation DNA binding analysis of purified nuclear receptors in the presence or absence of HMG-1/-2. The concentrations of purified receptors indicated in the figure were varied in DNA binding reactions against a constant amount (0.3 ng) of 32P-labeled oligonucleotide probe, and DNA binding was analyzed by EMSA. Each receptor was assayed alone (HMG-1 or HMG-2) or with the addition of 150 to 300 ng of HMG-1, HMG-2, or ovalbumin (+OVAL). The oligonucleotide probe for PR-A (panel A), GR (panel B), and AR (panel C) was a palindromic GRE-PRE. A palindromic ERE oligonucleotide was used as the probe for ER (panel D), a DR-3 oligonucleotide was used for the VDR-RXR heterodimers (panel E), and a DR-1 probe was used for the RXR homodimers (panel F). Purified receptors were quantitated by protein Bradford assay and by comparing silver-stained receptor band intensities with those of known amounts of purified molecular weight protein standards. The specific DNA complexes at each concentration of receptor were quantitated by phosphorimage analysis and plotted as the percentage of total DNA.

HMG-1/-2 have no effect on the binding of nonsteroid nuclear receptors to direct-repeat target DNA sequences. We next examined the influence of HMG-1/-2 on binding of human VDR and RAR to DR target DNA sequences. EMSA showed that purified VDR did not bind to a DR-3 probe (Fig. 6A) and that purified RAR failed to interact with a DR-5 probe (Fig. 6B). In contrast to the results with steroid receptors, the addition of HMG-1 or HMG-2 had no effect on the DNA binding activities of VDR and RAR (Fig. 6). As expected, the addition of purified RXR stimulated the DNA binding activity of purified VDR and RAR by a heterodimerization mechanism, as shown by supershifts with RXR-, VDR-, and RAR-specific antibodies (Fig. 6). Competition with excess unlabeled homologous and unrelated oligonucleotides demonstrates that the RXR-induced complexes are specific (Fig. 6A and B). The addition of HMG-1 or HMG-2 also had no effect on the DNA binding activity of RXR-VDR and RXR-RAR heterodimers (Fig. 6A and B). To determine whether HMG-1/-2 might in fact stimulate this class of nonsteroid nuclear receptors but simply require a higher concentration than is needed to stimulate steroid receptors, increasing amounts of HMG-2 were added to VDR-RXR heterodimer DNA binding reactions. As shown in Fig. 6C, at concentrations three times higher (900 ng) than are required to maximally stimulate PR-DNA binding, HMG-2 had no effect on the sequence-specific DNA binding activity of VDR-RXR heterodimers (Fig. 6C). Also, the HMG-2 antibody did not supershift VDR-RXR DNA complexes in the presence of large amounts of HMG-2, suggesting that HMG-2 is not recruited to the complex (Fig. 6C).

View larger version (46K): [in this window] [in a new window]   View larger version (42K): [in this window] [in a new window]   View larger version (58K): [in this window] [in a new window]   FIG. 6.   HMG-1/-2 has no influence on sequence-specific DNA binding activity of purified human VDR and RAR. (A) Human VDR expressed and purified from yeast cells was incubated for 1 h at 4°C with 0.3 ng of a [32P]DR-3 probe, and samples were analyzed by EMSA. A single concentration of purified VDR (60 nM) was incubated alone () or with the addition of 150 ng of purified HMG-1, 1 µg of ovalbumin (Oval), 50 ng of purified RXR, or 50 ng of RXR plus 150 ng of purified HMG-1. A supershift of RXR-induced DNA complexes with an antibody to RXR (RXRab) shows that RXR is a component of the stimulated DNA complex. The specificity of the DNA complexes is shown by the lack of competition with a 100-fold molar excess of an unlabeled nonspecific DNA (100×ns) compared to an unlabeled DR-3 (100×DR-3). (B) Purified human RAR (60 nM) expressed and purified from yeast cells was incubated with 0.3 ng of a [32P]DR-5 probe, and samples were analyzed by EMSA as for panel A. (C) Purified VDR and RXR were incubated with the DR-3 probe as for panel A. Supershifts of the RXR-VDR heterodimer-DNA complex are shown with antibodies to VDR (VDR ab) and RXR (RXR ab). Increasing amounts of purified HMG-2 (300, 600, and 900 ng) were added to the VDR-RXR heterodimer-DNA binding reaction. At the intermediate amount of HMG-2 (i.e., 600 ng), DNA binding reactions were incubated with two concentrations of HMG-2 antibody (HMG-2 ab) or a control unrelated antibody (Control ab).

HMG-1 and HMG-2 transiently interact with PR and are recruited by PR to the DNA complex. To further investigate the mechanism by which HMG-1/-2 stimulate the apparent binding affinity of steroid receptors for HREs, we focused our analysis on PR. Using short PRE oligonucleotide probes, we previously observed that stimulation of PR-DNA binding by HMG-1 was not accompanied by a decrease in electrophoretic mobility of the DNA complex, nor were stimulated complexes supershifted by the addition of antibodies to HMG-1 (42, 46). This suggested that HMG-1 is a less-stable component of the DNA complex than PR and that it dissociates during electrophoresis. However, in the context of a longer DNA fragment containing a single PRE, the addition of HMG-1 did result in reduced mobility of some of the stimulated PR-DNA complexes, which was further supershifted by anti-HMG-1 MAb (47). To test whether HMG-2 can also associate with the PR-DNA complex, we used a longer PRE oligonucleotide probe than that used in Fig. 3 to 5 (43 bp versus 32 bp). As shown in Fig. 7A, purified HMG-2 alone did not bind to the 43-bp PRE probe at any of the concentrations tested, nor were complexes formed with purified PR alone at a submaximal receptor concentration. The addition of purified PR and HMG-2 together resulted in a synergistic stimulation of DNA complexes that contained both HMG-2 and PR, as evidenced by supershifts with PR (AB-52)- or HMG-2-specific antibodies (Fig. 7A). As a control, an antibody (h151) to ER had no effect on the electrophoretic mobility of the complex. These results show that HMG-2 can associate with the PR-DNA complex. Furthermore, the inability of HMG-2 to associate with the PRE until receptor was added indicates that HMG-2 is recruited to the complex by PR.

View larger version (19K): [in this window] [in a new window]   FIG. 7.   Association of HMG-2 with the PR-DNA complex and direct binding of HMG-1/-2 to PR in the absence of DNA. (A) HMG-2 is recruited to the PR-DNA complex. A 43-bp oligonucleotide containing the distal-most GRE-PRE and flanking sequences of MMTV was used as the DNA probe for EMSA. The DNA probe (0.3 ng) was incubated with increasing amounts of purified HMG-2 alone (100 to 300 ng), with purified PR-A alone (30 nM), or with purified PR-A (30 nM) plus 300 ng of HMG-2, either without (none) or with antibody to PR (AB-52), ER (h151), or HMG-2 (HMG-2ab; rabbit antisera added in increasing amounts). (B) (Left panel) Baculovirus-expressed polyhistidine-tagged HMG-1 was incubated with nonfusion recombinant PR-B, and the samples were bound to Talon resins. Resins were washed six times in a buffer containing 100 mM NaCl and 15 mM imidazole, and bound proteins were eluted with SDS-sample buffer and analyzed by Western blotting with the 1294 MAb to PR. As a control for nonspecific binding, PR-B was incubated with Talon resins in the absence of polyhistidine-tagged HMG-1. As a positive control for a known interacting protein, PR-B was incubated with polyhistidine-tagged SRC-1. Purified PR-B (middle panel) or VDR (right panel) was incubated with calf thymus HMG-2, and samples were immunoprecipitated with a rabbit antibody to HMG-2 by using protein A-Sepharose as an absorbent. The protein A-Sepharose beads were washed six times in a buffer containing 100 mM NaCl, and bound proteins were eluted with SDS-sample buffer and analyzed by Western blotting with the 1294 MAb to PR (middle panel) or the 9A7 MAb to human VDR (right panel). The leftmost lane in each of these two panels is the assay input of PR-B or VDR.

HMG-1/-2 stimulate PR-mediated gene activation in mammalian cells. To determine whether HMG-1/-2 can affect the transcriptional activity of PR in vivo, COS1 cells were cotransfected with expression plasmids for hPR-B, HMG-1, or HMG-2, and a progesterone-responsive reporter construct containing two GREs-PREs fused to the TATA box of E1b and the CAT reporter gene (DHRE-E1b-CAT). PR-B in general is a stronger transcriptional activator than PR-A; however, this differential activity is both cell-type and promoter context specific (64, 69). With this simple GRE-PRE promoter, PR-B in COS1 cells is a much stronger activator than PR-A (not shown). Thus, our studies have focused on the effect of HMG-1/-2 on the activity of PR-B. When COS1 cells were transfected with phPR-B alone, addition of the synthetic progestin R5020 resulted in an ~10-fold stimulation of CAT activity (Fig. 8A). Upon cotransfection with phPR-B and increasing amounts of the HMG-1 expression plasmid (pHMG-1), R5020 stimulation of CAT activity was increased in a dose-dependent manner by another sevenfold at the highest amount of transfected pHMG-1 (7.03-fold ± 1.9 standard error of the mean [SEM], n = 11). Cotransfection with an empty vector control had minimal effect on R5020 stimulation of the DHRE-E1b-CAT reporter gene (Fig. 8A). In the absence of hormone small increases in reporter gene activity were observed when cotransfecting cells with phPR-B and pHMG-1. The increase in R5020 induction by cotransfected HMG-1 is PR dependent and is not due to an effect on the basal promoter activity of the reporter gene. This is shown by the lack of stimulation of CAT activity when cells were transfected with pHMG-1 in the absence of hPR-B (Fig. 8A). These results demonstrate that cotransfection of HMG-1 substantially increases hormone-dependent PR-mediated transactivation without affecting the basal promoter activity of the reporter gene.

View larger version (35K): [in this window] [in a new window]   FIG. 8.   Ectopically expressed HMG-1 or HMG-2 stimulates the transcriptional activity of human PR in mammalian cells. (A) COS1 cells were transfected with a PR-B expression plasmid (phPR-B; 0.5 ng/well), increasing amounts of an HMG-1 expression plasmid (pHMG-1; 1 to 20 ng/well) or an empty vector control (1 to 20 ng/well), and the DHRE-E1b-CAT reporter plasmid (500 ng/well). Cells were treated with or without R5020 (100 nM) for the last 24 h of transfection. In some wells, cells were cotransfected with increasing amounts of pHMG-1 (1 to 20 ng/well) and no PR-B (PR-B). CAT activity was calculated as counts per minute of 3H-labeled acetyl coenzyme A converted per microgram of total protein, and the values shown are averages from three independent experiments. (B) COS1 cells were transfected with various amounts of phPR-B expression plasmid in the presence or absence of pHMG-1 (20-fold molar excess over phPR-B). At 24 h after transfection, cells were treated for the next 24 h with or without R5020 (100 nM), and CAT activity was measured and calculated as the ratio of CAT activity to -Gal activity of the internal control plasmid. The results are average values from duplicate transfection wells from a single experiment and are representative of two independent experiments. (C) COS1 cells were transfected with phPR-B (1 ng/well), DHRE-E1b-CAT reporter (500 ng/well), various amounts of pHMG-1 and pHMG-2, or an empty control vector (0.5- to 20-fold molar excess over the PR expression plasmid). The CAT activity in each treatment group was calculated as the fold R5020 induction over that with no hormone. The data were expressed as relative CAT activity, setting the hormone-induced level obtained in cells transfected with PR alone as the 100% value. The values are averages from four independent experiments. (D) HeLa cells were transfected with phPR-B (10 ng/well), increasing amounts of either pHMG-1 or the empty control vector (1.0- to 20-fold molar excess over phPR-B), and the DHRE-E1b-CAT (500 ng/well). The relative CAT activity was calculated as for panel C. The values are averages from duplicate transfections from a single experiment that is representative of three independent experiments.

HMG-1 stimulates transcriptional activity of other steroid receptors (GR and AR) in vivo but has minimal effect on VDR. We also tested, by use of transient-transfection assays, the effect of HMG-1 on the transcriptional activity of other nuclear receptors in vivo. Because PR, GR, and AR can all bind and mediate functional responses through the same consensus hormone response element, the PRE₂-tk-LUC reporter vector was used to assess the effect of HMG-1 on transcriptional activation mediated by GR and AR. Both AR- and GR-mediated activation of PRE₂-tk-LUC was increased substantially in cells that were cotransfected with pHMG-1 compared to cells cotransfected with receptor alone or with receptor and an empty vector control (Fig. 9A). The only difference from results obtained with PR was a greater stimulation of ligand-independent activation of AR and GR by HMG-1 (Fig. 9A). To determine whether HMG-1 affects the activity in vivo of a receptor from the nonsteroid class of nuclear receptors, COS1 cells were cotransfected with a VDR expression plasmid and increasing amounts of pHMG-1, and the ability of vitamin D₃ to induce expression of VDRE-promoter constructs was assessed. Since COS1 cells express endogenous RXR, these assays measured the transcriptional activity of the RXR-VDR heterodimers. At the two lowest amounts of cotransfected plasmids, pHMG-1 had no effect on vitamin D₃-induced, VDR-dependent activation of the VDRE-24(OH)ase-LUC reporter gene (Fig. 9B). However, at the highest amount of cotransfected plasmid, pHMG-1 stimulated VDR-mediated gene activation by 2.6-fold (2.6 ± 0.73 SEM, n = 7). Within the same experiments, cotransfection with pHMG-1 increased R5020-dependent, PR-mediated activation of the PRE-tk-LUC reporter gene by 10-fold (Fig. 9B). Because VDRE-24(OH)ase-LUC is a complex promoter with potential sites for other transactivators that could be responsible for the 2.6-fold enhancement of VDR activity by pHMG-1, we have also analyzed the effect of HMG-1 on the VDR-dependent activation of two other reporters, including VDRE-1MTV-LUC and DR3-tk-LUC. Ectopically expressed HMG-1 had no stimulatory effect on VDR transactivation of either of these vitamin D₃-responsive reporter constructs, even with amounts of cotransfected pHMG-1 10 times greater than that required to maximally enhance PR activity in vivo (Fig. 9C and D). Again, coexpressed HMG-1, as an internal positive control, stimulated PR activity within the same transient transfection assays by ca. 10-fold (Fig. 9C and D). Thus, consistent with the effects of HMG-1/-2 on receptor-DNA binding in vitro, ectopically expressed HMG-1/-2 substantially increased the transcriptional activity of steroid receptors in vivo but had minimal [24(OH)ase promoter] or no (VDRE-1MTV-LUC and DR3-tk-LUC) effect on the activity of at least one other class of nuclear receptor.

View larger version (34K): [in this window] [in a new window]   FIG. 9.   HMG-1 stimulates transcriptional activity of other steroid receptors in vivo but has minimal effects on VDR-mediated transcription. COS1 cells were cotransfected with human AR (p5HBL-AR-A) or GR (pSVGR) expression plasmids (10 ng/well), pHMG-1 (400 ng/well) or an empty vector control, and the PRE2-tk-LUC (200 ng/well) reporter. Cells were incubated for 24 h prior to harvest with vehicle, dexamethasone (Dex.; 106 M) or dihydrotestosterone (DHT) (107 M). LUC was measured and calculated as relative activity, setting the hormone-induced levels in cells transfected with receptor alone (plus empty vector control) at 100%. The values are averages from three independent experiments for AR and from four independent experiments for GR. (B) COS1 cells were transfected with a human VDR expression plasmid (1 ng/well), increasing amounts of pHMG-1 or an empty vector control (4- to 40-fold excess over VDR), and the VDRE-24(OH)ase-LUC reporter (200 ng/well). At 24 h after transfection, cells were treated for another 24 h without and with 1,25-(OH)2 D3 (10 nM). Within the same experiments, COS1 cells were cotransfected with phPR-B (1 ng/well), pHMG-1 or empty vector (40 ng/well), and PRE2-tk-LUC (200 ng/well). At 24 h after transfection, the cells were treated for another 24 h without and with R5020 (10 nM). LUC activity was measured and calculated as the relative activity as for panel A. Values are averages from seven independent experiments. (C and D) COS1 cells were cotransfected with VDR expression plasmid (1 ng/well), increasing amounts of pHMG-1, or empty control vector (4- to 400-fold excess over VDR) and VDRE-1MTV-LUC (200 ng/well) or DR3-tk-LUC (200 ng/well) reporter plasmids. Cells were treated for the last 24 h prior to harvest with 10 mM 1,25-(OH)2 D3, and luciferase was assayed and calculated as the relative activity as for panels A and B. Within the same transfections, the effect of pHMG-1 on the PR-mediated activation of PRE2-tk-LUC was assessed as for panel B. The values are averages from four independent experiments for VDRE-1MTV-LUC and from two independent experiments for DR3-tk-LUC.

	DISCUSSION

Top Abstract Introduction Materials & Methods Results Discussion References

In previous studies we showed that HMG-1 markedly and specifically increased the binding affinity of PR for target DNA in vitro (42, 46). We now show by transient-cotransfection assays that HMG-1 and the closely related HMG-2 can also stimulate the transcriptional activity of PR in mammalian cells. This increase in PR activity was observed in two different cell types and with two different target promoters, suggesting a generality to the in vivo functional interaction between HMG-1/-2 and PR. The major effect of ectopically expressed HMG-1 and HMG-2 was to increase the transcriptional activity of the receptor-hormone complex without affecting the basal promoter activity of the reporter gene (Fig. 8A). It also appears that coexpression of HMG-1/-2 directly stimulates PR-mediated transcription as opposed to altering the cellular levels of expressed PR or the copy number of the reporter gene plasmid. As determined by Western blots and whole-cell-binding assays, cotransfection of COS1 cells with pHMG-1 and phPR-B did