INTRODUCTION

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Mitogen-activated protein kinases (MAPKs) are components of pathways that relay signals to particular cell compartments in response to a diverse array of extracellular stimuli (38, 42, 63, 83). Activated MAPK can translocate to the nucleus and phosphorylate substrates, including transcription factors, thereby eliciting a biological response. At least three groups of MAPKs have been identified in mammals: ERK (extracellular signal-regulated kinase), JNK (c-Jun N-terminal kinase; also known as stress-activated protein kinase), and p38 MAPK (also known as cytokine-suppressive anti-inflammatory drug-binding protein). ERK contributes to the response of cells to signals initiated by many growth factors and hormones through a Ras-dependent pathway (63). In contrast, JNK and p38 MAPK are activated by environmental stresses, such as UV radiation, osmotic shock, heat shock, protein synthesis inhibitors, and lipopolysaccharide (38, 83). The JNK and p38 MAP kinases are also activated by treatment of cells with proinflammatory cytokines, including interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-) (38, 83). MAPKs are involved in the control of a wide spectrum of cellular processes including growth, differentiation, survival, and death (38, 63).

MAPKs are activated by conserved protein kinase signaling modules which include a MAPK kinase kinase (MAPKKK) and a dual-specificity MAPK kinase (MAPKK). The MAPKKK phosphorylates and activates the MAPKK which, in turn, activates the MAPK by dual phosphorylation on threonine and tyrosine residues within a Thr-Xaa-Tyr motif located in protein kinase subdomain VIII (38, 63). Separate protein kinase signaling modules are used to activate different groups of MAPKs (13). The MAPKKK and MAPKK that activate the ERK MAP kinases include c-Raf-1 and MEK1, respectively (63). The c-Raf-1 protein kinase activity is regulated by the small GTPase Ras, which induces translocation of c-Raf-1 to the plasma membrane, where it is thought to be activated (63). In contrast, JNK and p38 MAPK appear to be activated by small GTPases of the Rho family (3, 10, 49, 59, 91). The mechanism by which Rho GTPases activate the JNK and p38 MAPK signaling pathways is unclear. Although Rho GTPases interact with the PAK group of STE20-related protein kinases, it appears that JNK and p38 MAP kinase activation may be mediated, in part, by the mixed-lineage group of protein kinases (MLK) (62, 74) or by the scaffold protein POSH (72). STE20-like protein kinases represent possible targets for other upstream signals that lead to JNK activation. Among the STE20-like protein kinases, the hematopoietic progenitor kinase 1 (HPK1) (2, 37, 41, 79) and the kinase homologous to STE20/SPS1 (KHS) (78) appear to specifically activate JNK. There is evidence for significant complexity in the mechanism of initiation of the JNK and p38 MAPK signaling pathways because of the large number of MAPKKK protein kinases that contribute to stress-activated MAPK signaling (19, 38). Whether there is a general or a specific role for Rho family GTPases in the activation of the JNK and p38 MAP kinase signaling pathways has not been established.

The protein kinases that have been reported to act as MAPKKKs for the JNK signaling pathway include the MEK/ERK kinase (MEKK) group, the MLK group, TPL-2, ASK1, and TAK1 (19, 38). The MEKK group of MAPKKKs includes MEKK1 (43, 50, 86), MEKK2 (6, 11), MEKK3 (6, 11, 15), MEKK4/MTK1 (25, 71), and MAPKKK5/MEKK5 (80). The MLK group of MAPKKKs includes MLK1 (14), MLK2/MST (14, 33), MLK3/SPRK (62, 74, 75), dual-leucine zipper-bearing kinase (DLK)/MUK (18, 32), and leucine zipper-bearing kinase (LZK) (64). Binding sites for the Rho family GTPases Cdc42 and Rac1 have been described for MLK1, MLK2, and MLK3 (but not DLK or LZK) (7). It has also been reported that MEKK1 and MEKK4 (but not MEKK2 or MEKK3) bind directly to the Rho family GTPases Cdc42 and Rac1 (20). These interactions between Rho GTPases and MAPKKKs may contribute to the effects of Rho GTPases on JNK activation. MAPKKK protein kinases that do not interact with Rho GTPases may mediate the effects of Rho-independent signals that lead to JNK activation.

Several MAPKKs have been identified. ERK is activated by MEK1 and MEK2 (1); p38 MAPK is activated by MKK3 (13), MKK4 (13, 45), and MKK6 (28, 53, 61, 68); JNK is activated by MKK4 (13, 45, 65). The existence of JNK activators distinct from MKK4 was suggested by chromatographic fractionation of cell extracts (48, 52) and by the results of targeted disruption of the MKK4 gene (58, 87). We (76) and others (21, 34, 36, 44, 46, 55, 77, 84, 89) have recently characterized the novel JNK activator MKK7. In contrast to MKK4, which activates both JNK and p38 MAPK, the MKK7 protein kinase selectively activates only JNK. Interestingly, the two JNK activators D-MKK4 (Drosophila MKK4) (29) and Hep/D-MKK7 (26, 67) are conserved in Drosophila. Genetic analysis demonstrates that D-MKK4 and Hep/D-MKK7 serve nonredundant functions in the fly (38). Similarly, gene disruption experiments demonstrate that MKK4 is an essential gene in the mouse, indicating that MKK7 is unable to fully substitute for the function of MKK4 (24, 57, 58, 70, 87). These data demonstrate that MKK4 and MKK7 serve nonredundant functions as activators of the JNK MAP kinases in vivo.

We report here the molecular cloning of the MKK7 gene and the characterization of MKK7 isoforms that are created by alternative splicing. The MKK7 isoforms are differentially activated by upstream signals, and their regulation differs from that of the MKK4 isoforms. These data establish that JNK activation is mediated by a family of protein kinases that are formed by the alternative splicing of two genes.

	MATERIALS AND METHODS

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Materials. Human TNF- and IL-1 were from Genzyme Corp. [-³²P]ATP was obtained from Amersham Corp. Recombinant glutathione S-transferase (GST)-c-Jun and GST-JNK1 were purified from bacteria as described elsewhere (12). Mammalian expression vectors for MKK4 isoforms (13) and MKK71 (76) have been described elsewhere. MKK4 includes an additional 34 amino acids fused to the NH₂ terminus of MKK4 (13). The expression vectors pCMV-HA-MEK1 and pCMV-HA-N3-MEK1-EE were provided by N. Ahn (47). The expression vectors pEBG-KHS (78), pSR-HPK1 (37), pMT2T-MEKK3 (15), pCDNAI-MTK1/MEKK4 (71), pCDNA3-MLK3 (74), and pSR-DLK (33) were provided by J. Blenis, T. Tan, U. Siebenlist, H. Saito, S. Gutkind, and J. Avruch, respectively.

Molecular cloning of MKK7 isoforms. Genomic DNA clones corresponding to the MKK7 locus were isolated by screening a  FixII murine genomic library (Stratagene, Inc.), using the MKK71 cDNA as a probe. The genomic sequence of MKK7 was obtained with an Applied Biosystems model 373A machine. To identify additional MKK7 isoforms, a mouse testis cDNA library cloned in the phage ZAPII (Stratagene, Inc.) was screened by using the MKK71 cDNA as a probe. Positive clones were plaque purified and sequenced. The MKK7 isoforms were subcloned into the mammalian expression vectors pCDNA3 (Invitrogen Inc.) and pEBG (86). The Flag epitope (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys; Immunex Corp.) was fused to the NH₂ terminus of the MKK7 isoforms by insertional overlapping PCR (35). A nuclear export signal (NES) sequence (23) corresponding to residues 32 to 51 of MEK1 (Ala-Leu-Gln-Lys-Lys-Leu-Glu-Glu-Leu-Glu-Leu-Asp-Glu-Gln-Gln-Arg-Lys-Arg-Leu-Glu) was inserted following the Flag epitope, by using a PCR-based procedure to create NES-MKK7 isoforms. Bacterial expression of MKK7 was performed with the vector pGEX-4T-1 (Pharmacia-LKB Biotechnology, Inc.).

FISH. Lymphocytes isolated from male mouse spleen were used for chromosome fluorescence in situ hybridization analysis (FISH) (30, 31). Metaphase chromosomes spread on a glass slide were air dried, baked at 55°C (1 h), and digested with RNase A. The DNA was denatured for 2 min at 70°C in 70% formamide in 2× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate) and dehydrated with ethanol. DNA prepared from a mouse MKK7 genomic clone was biotinylated and used as the hybridization probe (30). The FISH and 4',6-diamidino-2-phenylindole (DAPI) staining were recorded on film. The assignment of the FISH mapping data with chromosomal bands was achieved by superimposing the FISH signals with images of the DAPI-banded chromosomes.

Tissue culture and transfection assays. COS-7 and 293 cells were cultured at 37°C in Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated fetal bovine serum (Life Technologies, Inc.), 2 mM glutamine, 100 U of penicillin per ml, and 100 U of streptomycin per ml in a humidified environment with 5% CO₂. Transient transfections were performed with the LipofectAMINE reagent (Life Technologies) according to the manufacturer's recommendations. After 36 h, the cells were serum starved for 1 h and treated with UV-C (80 J/m²), anisomycin (5 µg/ml), TNF- (20 ng/ml), or IL-1 (2 to 10 ng/ml) for 30 min prior to lysis. Cell extracts were prepared in lysis buffer (20 mM Tris [pH 7.4], 10% glycerol, 1% Triton X-100, 0.137 M NaCl, 25 mM -glycerophosphate, 2 mM EDTA, 1 mM sodium orthovanadate, 10 µg of leupeptin per ml, 10 µg of aprotinin per ml, 1 mM phenylmethylsulfonyl fluoride) and centrifuged at 15,000 × g (15 min at 4°C). The concentration of total soluble protein in the supernatant was quantitated by the Bradford method (Bio-Rad).

Binding assays. GST-tagged MKK7 proteins were isolated by incubation with glutathione (GSH)-Sepharose (Pharmacia-LKB Biotechnology) in lysis buffer (4 h at 4°C). The beads were washed five times with lysis buffer, and the presence of bound JNK was examined by immunoblot analysis.

Protein kinase assays. Epitope-tagged MAPKK was immunoprecipitated from cell extracts by incubation for 3 h at 4°C with the Flag-specific monoclonal antibody M2 (IBI-Kodak) bound to protein G-Sepharose beads (Pharmacia-LKB Biotechnology). GST-tagged MAPKK was isolated by incubation for 3 h at 4°C with GSH-Sepharose beads (Pharmacia-LKB Biotechnology). The complexes were washed twice with lysis buffer and three times with kinase buffer (25 mM HEPES [pH 7.4], 25 mM -glycerophosphate, 25 mM MgCl₂, 0.5 mM dithiothreitol, 0.1 mM sodium orthovanadate). MAPKK activity was measured at 30°C for 20 min in 30 µl of kinase buffer containing 0.5 µg of GST-JNK1, 2 µg of GST-c-Jun, and 50 µM [-³²P]ATP (10 Ci/mmol; 1 Ci = 37.6 GBq). The reactions were terminated by addition of Laemmli sample buffer. Proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE 10% polyacrylamide gel) and identified by autoradiography. The incorporation of [³²P]phosphate into GST-c-Jun was quantitated by PhosphorImager analysis.

Western blot analysis. Proteins were resolved by SDS-PAGE (10% gel), electrophoretically transferred to an Immobilon-P membrane (Millipore Inc.), and probed with the Flag-specific monoclonal antibody M2 (1:4000; IBI-Kodak) or a monoclonal antibody to MKK4 (1:1000; Pharmingen). Immune complexes were detected by enhanced chemiluminescence (Lumiglo; Kirkegaard & Perry Laboratories).

Immunofluorescence microscopy. COS-7 cells were seeded onto glass coverslips coated with poly-L-lysine (Sigma Chemical Co.); 36 h after transfection, the cells were fixed for 10 min with 4% paraformaldehyde in phosphate-buffered saline (PBS) and then permeabilized for 5 min with 0.2% Triton X-100 in PBS. After incubation for 15 min with 3% (wt/vol) bovine serum albumin (BSA) in PBS, the coverslips were incubated for 1 h with primary antibodies in PBS with 3% BSA. The primary antibodies were rabbit anti-MKK4 (K-18; 1:100; Santa Cruz Biotechnology), goat anti-MKK7 (C-18; 1:100; Santa Cruz), rabbit anti-phosphorylated (on Thr-183 and Tyr-185) JNK (phospho-JNK) (9251; 1:100; New England Biolabs Inc.), and monoclonal antibodies (1:500) to Flag (M2) and HA (12CA5). Immune complexes were detected with Texas red-conjugated anti-mouse immunoglobulin (Ig), Texas red-conjugated anti-goat Ig, rhodamine-conjugated anti-rabbit Ig, and fluorescein-conjugated anti-rabbit Ig antibodies (1:100; Jackson ImmunoResearch, Inc.) in 3% BSA in PBS. After nuclei were stained for 1 min with DAPI (1:10,000; Molecular Probes, Inc.), the coverslips were mounted in Vectashield (Vector Laboratories, Inc.). All procedures were performed at room temperature. Fluorescence microscopy was performed with a Zeiss Axioplan microscope.

Nucleotide sequence accession numbers. The sequences of the murine MKK7 cDNA clones have been deposited in GenBank with accession no. U93030, AF060943, AF060944, AF060945, AF060946, and AF060947.

	RESULTS

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Molecular cloning of MKK7 isoforms. MKK7 has recently been identified as a specific activator of the JNK group of MAPKs. Northern blot analysis demonstrated the presence of at least two MKK7 mRNAs (2.2 and 4.2 kb) in adult murine tissues (76), an observation which suggested that MKK7 may be expressed as a group of alternatively spliced isoforms. To characterize members of the MKK7 group, we exhaustively screened a mouse testis cDNA library by using our original MKK7 clone (MKK71) as a probe. This analysis led to the identification of additional isoforms: MKK72, MKK71, MKK72, MKK71, and MKK72 (Fig. 1). The predicted amino acid sequences are identical except at the NH₂ terminus (, , and  isoforms) and at the COOH terminus (1 and 2 isoforms).

View larger version (42K): [in this window] [in a new window]   FIG. 1.   Primary structures of MKK7 protein kinase isoforms. The primary sequence of the mouse MKK7 protein kinase isoforms deduced from the sequences of cDNA clones are presented in single-letter code. Residues that are identical to those in MKK72 (.), deletions (), and termination codons (#) are indicated.

View larger version (50K): [in this window] [in a new window]   FIG. 2.   The MKK7 gene is located on mouse chromosome 11. The MKK7 gene was identified by FISH analysis of murine metaphase chromosomes by using an MKK7 genomic clone as a probe. The FISH signal is illustrated on the left (arrow). The corresponding DAPI signal indicating chromosome 11 is shown on the right. Detailed comparison of DAPI-banded chromosome 11 with the FISH signal indicated that the MKK7 gene is located in region A2.

View larger version (16K): [in this window] [in a new window]   FIG. 3.   Schematic representation of the structure of the MKK7 gene. The MKK7 gene is formed by 14 exons. The alternative splicing that creates the , , , 1, and 2 isoforms of MKK7 is illustrated. The coding (black) and noncoding (grey) regions and excluded exons (striped) are shown. Initiation codons (ATG), termination codons (*), and polyadenylation signals () are indicated.

Comparison of protein kinase activities of MKK7 isoforms. We used a coupled protein kinase assay in vitro to examine the protein kinase activities of MKK7 isoforms that were isolated from transfected mammalian cells. This assay employs bacterially expressed JNK1 as a substrate for the MKK7 protein kinases. The activation of JNK1 by MKK7 was examined by using bacterially expressed c-Jun as a substrate for JNK. Control experiments indicated that MKK7 does not phosphorylate c-Jun (data not shown). These assays demonstrated that the activities of the MKK7 and MKK7 isoforms were similar and that the alternative splicing at the COOH terminus of MKK7 (1 and 2 isoforms) did not markedly alter protein kinase activity (Fig. 4). In contrast, activities of the MKK7 isoforms were lower than those of the MKK7 and MKK7 isoforms. Quantitation of the MKK7 activity by PhosphorImager analysis indicated that MKK71 was 37-fold more active than MKK71.

View larger version (26K): [in this window] [in a new window]   FIG. 4.   Effects of MKK7 homologs on JNK activation. (A) COS-7 cells were transfected with expression vectors encoding Flag epitope-tagged MKK71, MKK72, MKK71, MKK72, MKK71, or MKK72. The expression of each of these MKK7 isoforms was examined by immunoblot (IB) analysis using the Flag-specific monoclonal antibody M2. The MKK7 isoforms were immunoprecipitated, and their activities were measured in the immune complex by a coupled protein kinase assay (KA) using recombinant GST-JNK1 and GST-c-Jun as substrates. The phosphorylated c-Jun was detected after SDS-PAGE by autoradiography and was quantitated by PhosphorImager analysis. The effect of cotransfection with an empty expression vector () or with an MEKK1 expression vector (+) is shown. Similar results were observed in three separate experiments. (B) MKK7 activity, presented as relative protein kinase activity. (C) MKK7 activation caused by MEKK1 (fold activity over MKK7 activity in the absence of MEKK1).

The NH₂-terminal region of MKK7 interacts with JNK. The observation that activities of the MKK7 isoforms were lower than those of the MKK7 and MKK7 isoforms suggests that these MKK7 isoforms may differentially interact with the substrate JNK. To test this hypothesis, we examined the interaction of MKK7 and MKK7 isoforms with JNK1 following expression in COS-7 cells. We precipitated the MKK7 protein kinases from cell lysates, and examined the presence of JNK1 in the precipitates by immunoblot analysis (Fig. 5A). These assays demonstrated that MKK7 isoforms, but not MKK7 isoforms, coprecipitated with JNK. This binding interaction may contribute to the higher activities of MKK7 isoforms than of MKK7 isoforms (Fig. 4).

View larger version (15K): [in this window] [in a new window]   FIG. 5.   Interaction of JNK with MKK7 isoforms. (A) Selective binding of MKK7 isoforms to JNK. Epitope-tagged JNK1 and either GST (Control) or GST-tagged MKK71, MKK72, MKK71, or MKK72 were expressed in COS-1 cells. Protein expression levels, monitored by immunoblot analysis of the cell lysates, were similar in all transfections. The MKK7 protein kinases were isolated from cell extracts by incubation with GSH-agarose. The binding of JNK1 was examined by immunoblot analysis with an antibody to the HA epitope tag. (B) JNK binds to the NH2 terminus of MKK7. Bacterially expressed GST (Control) or a GST fusion protein (residues 1 to 73 of MKK7) was immobilized on GSH-agarose and incubated with extracts prepared from COS-7 cells expressing epitope-tagged JNK1. The agarose beads were washed, and the amount of bound JNK1 was examined by immunoblot analysis.

Regulation of MKK7 isoforms by MAPKKKs. The protein kinases MEKK1, MLK3, and DLK preferentially activate JNK (32, 33, 50, 62, 74, 75, 86), MEKK3 activates both JNK and ERK (6, 11, 15), while MEKK4 has been reported to activate both JNK and p38 MAPK (25, 71). Since MEKK1 causes differential activation of MKK7 isoforms (Fig. 4), we examined whether other MAPKKKs, including members of the MEKK and MLK groups (19), could also cause differential activation of MKK7 isoforms.

View larger version (44K): [in this window] [in a new window]   FIG. 6.   Effect of MEKK and STE20 protein kinases on JNK activation. To examine the ability of MAPKKKs and STE20 homologs to activate JNK1, COS-7 cells were cotransfected with a mammalian expression vector encoding HA-tagged JNK1 together with an empty expression vector (Control) or an expression vector encoding either MEKK1, MEKK3, MEKK4, MLK3, DLK, KHS, or HPK1. The expression of JNK1 was examined by immunoblot (IB) analysis using a monoclonal antibody to the HA epitope tag. JNK1 was immunoprecipitated and its activity was measured in the immune complex by a protein kinase assay (KA) using recombinant GST-c-Jun as the substrate. The phosphorylated c-Jun was detected after SDS-PAGE by autoradiography and was quantitated by PhosphorImager analysis. JNK activity is presented as relative protein kinase activity. The levels of JNK activation caused by MEKK1, MEKK3, MEKK4, MLK3, DLK, KHS, and HPK1 were 28-, 29-, 2.0-, 24-, 30-, 5.3-, and 4.3-fold, respectively. Similar results were obtained in three separate experiments.

View larger version (44K): [in this window] [in a new window]   FIG. 7.   Activation of MKK4 and MKK7 protein kinases by MAPKKKs. To examine the abilities of MAPKKKs to activate MKK isoforms, COS-7 cells were cotransfected with mammalian expression vectors encoding tagged MKK71, MKK72, MKK71, MKK72, MKK4, or MKK4 together with either an empty expression vector (Control) or an expression vector encoding MEKK1, MEKK3, MEKK4, MLK3, or DLK. MKK protein expression was monitored by immunoblot (IB) analysis. The MKK4 and MKK7 isoforms were immunoprecipitated, and their activities were measured by a coupled protein kinase assay (KA) using recombinant JNK1 and c-Jun as substrates. The phosphorylated c-Jun was detected after SDS-PAGE by autoradiography and was quantitated by PhosphorImager analysis. MAPKK activity is presented as relative protein kinase activity. The levels of MAPKK activation caused by MEKK1, MEKK3, MEKK4, MLK3, and DLK were 27-, 19-, 1.8-, 5.0-, and 7.0-fold (MKK71); 32-, 10-, 1.4-, 13-, and 6.5-fold (MKK72); 5.6-, 2.0-, 0.3-, 4.2-, and 4.5-fold (MKK71); 13-, 2.6-, 1.3-, 7.5-, and 5.1-fold (MKK72); 42-, 88-, 2.0-, 38-, and 39-fold (MKK4); and 53-, 77-, 2.2-, 48-, and 36-fold (MKK4), respectively. Similar results were obtained in three separate experiments.

Regulation of MKK4 and MKK7 isoforms by STE20-like protein kinases. Several STE20-like protein kinases have been reported to be capable of activating the JNK pathway (19). Among them, KHS (78) and HPK1 (37, 41, 79) appear to be specific for the JNK signaling pathway. We therefore examined the abilities of KHS and HPK1 to activate MKK4 and MKK7 isoforms. Cells were transiently transfected with expression vectors encoding epitope-tagged MKK4 or MKK7 isoforms, together with expression vectors for either KHS or HPK1. Control experiments demonstrated that the KHS and HPK1 protein kinases caused similar levels of JNK activation (Fig. 6). The amount of MKK4 and MKK7 protein expression was examined by Western blot analysis (Fig. 8). The MKK4 and MKK7 protein kinases were immunoprecipitated, and their activities were measured by a coupled protein kinase assay. KHS and HPK1 caused similar increases in the activities of MKK71, MKK72, and MKK4. However, differences in the activation of MKK4 and MKK7 isoforms were detected. Indeed, HPK1 was the most potent activator of MKK71 and MKK72, while KHS was the most potent activator of MKK4.

View larger version (30K): [in this window] [in a new window]   FIG. 8.   Activation of MKK4 and MKK7 protein kinases by STE20 homologs. To examine the abilities of KHS and HPK1 to activate MKK7 isoforms, COS-7 cells were cotransfected with Flag-tagged MKK71, MKK72, MKK71, MKK72, MKK4, or MKK4 together with an empty expression vector (Control) or an expression vector for either KHS or HPK1. Protein expression was monitored by immunoblot (IB) analysis. Flag-tagged MKKs were immunoprecipitated, and MKK activity was measured in the immune complex by a coupled protein kinase assay (KA) using recombinant JNK1 and c-Jun as substrates. The phosphorylated c-Jun was detected after SDS-PAGE by autoradiography and quantitated by PhosphorImager analysis. MAPKK activity is presented as relative protein kinase activity. The levels of MAPKK activation caused by KHS and HPK were 15- and 31-fold (MKK71); 3.9- and 15-fold (MKK72); 4.0- and 3.5-fold (MKK71); 5.4- and 4.4-fold (MKK72); 3.2- and 2.9-fold (MKK4); and 4.4- and 2.4-fold (MKK4), respectively. Similar results were obtained in three separate experiments.

Regulation of MKK4 and MKK7 protein kinases by extracellular stimuli. To identify the nature of the MKKs involved in the regulation of the JNK cascade in response to specific extracellular stimuli, we examined the effects of environmental stresses and proinflammatory cytokines on activation of MKK4 and MKK7 protein kinase activities. We found that MKK4 and MKK7 isoforms were selectively regulated by upstream kinases (Fig. 7 and 8). Therefore, we examined the effects of UV-C radiation, anisomycin, TNF-, and IL-1 on the regulation of MKK7 and MKK4 isoforms. The MKK4 and MKK7 isoforms were isolated, and their activity was measured by a coupled kinase assay. TNF and IL-1 selectively activated the MKK7 isoforms and only weakly activated MKK4 (Fig. 9). In contrast, UV-C radiation and anisomycin caused selective activation of MKK4 compared to MKK7 (Fig. 9). These data indicate that the MKK4 and MKK7 isoforms are selectively regulated by specific extracellular stimuli. This selective regulation is consistent with the genetic evidence for nonredundant roles of MKK4 and MKK7 in Drosophila and mammals (38).

View larger version (35K): [in this window] [in a new window]   FIG. 9.   Regulation of MKK4 and MKK7 protein kinases by extracellular stimuli. 293 cells were transiently transfected with expression vectors (pEBG) encoding either MKK71, MKK72, MKK71, MKK72, MKK4, or MKK4; 36 h after transfection, the cells were untreated (Control) or treated with UV-C (UV; 80 J/m2), anisomycin (ANISO; 5 µg/ml), TNF- (20 ng/ml), or IL-1 (2 ng/ml). The cells were harvested after incubation at 37°C (30 min). MKK4 and MKK7 were isolated by using GSH-Sepharose beads, and the MKK activity was measured by a coupled protein kinase assay using recombinant JNK1 and c-Jun as substrates. The radioactivity incorporated into c-Jun was quantitated after SDS-PAGE by PhosphorImager analysis. MAPKK activity is presented as relative protein kinase activity. The levels of MAPKK activation caused by UV, anisomycin, TNF-, and IL-1 were 1.4-, 1.5-, 2.2-, and 1.9-fold (MKK71); 2.8-, 1.8-, 3.9-, and 2.5-fold (MKK72); 2.4-, 2.1-, 2.5-, and 4.1-fold (MKK71); 2.0-, 1.7-, 2.8-, and 2.9-fold (MKK72); 4.7-, 3.2-, 0.9-, and 0.9-fold (MKK4); and 2.6-, 2.3-, 0.9-, and 0.9-fold (MKK4), respectively. Similar results were obtained in three separate experiments.

Subcellular localization of MKK4 and MKK7 protein kinases. JNK has been reported to accumulate in the nucleus upon treatment of cells with stimuli known to activate the JNK signaling pathway (8, 40, 51). We therefore examined the subcellular localization of MKK4 and MKK7 by immunofluorescence analysis. These studies demonstrated that the endogenous MKK4 and MKK7 protein kinases were distributed in both the cytoplasm and the nucleus (Fig. 10A). Exposure of the cells to UV-C radiation or treatment with IL-1 did not cause marked changes in the distribution of the endogenous MKK4 or MKK7 protein kinases.

View larger version (25K): [in this window] [in a new window]   FIG. 10.   Subcellular distribution of MAPKK isoforms. (A) Endogenous MKK4 and MKK7 (red) were detected by immunofluorescence analysis using primary antibodies specific for these MKK isoforms. The cells were untreated (Control) or treated (30 min) with UV (80 J/m2) or IL-1 (10 ng/ml). DNA was visualized by staining with DAPI (blue). The scale bar (white) represents 20 µm. (B) Epitope-tagged MKK71, MKK72, MKK71, MKK72, MKK4, MKK4, MEK1, and N3-MEK1-EE were detected by using a primary monoclonal antibody to the epitope tag and a Texas red-labeled secondary antibody. The scale bar (white) represents 10 µm.

Effect of nuclear exclusion on the activity of MKK7. The distribution of the MKK7 protein in the cytoplasm and the nucleus differs from that described for the ERK MAPK activator MEK1. While the endogenous MKK7 protein appears to preferentially accumulate in the nucleus (Fig. 10A), MEK1 is excluded from the nucleus. The mechanism of nuclear exclusion is mediated by the presence of an NES in the NH₂-terminal region of MEK1 (23). Disruption of the NES causes nuclear accumulation of MEK1 and marked potentiation of signaling (22). These data suggest that the NES may be important for the normal control of MEK1 activity.

View larger version (14K): [in this window] [in a new window]   View larger version (26K): [in this window] [in a new window]   View larger version (25K): [in this window] [in a new window]   FIG. 11.   Effect of an NES on the properties of MKK7. (A) Schematic representation of MKK7 and NES-MKK7. Both proteins were constructed with an NH2-terminal Flag epitope (grey box). The nuclear export signal (NES) of MEK1 (residues 32 to 51) was inserted following the Flag epitope to create NES-MKK7. (B) The MKK7 proteins and HA-JNK1 were expressed in COS-7 cells. Transfected cells were identified by immunofluorescence analysis using antibody M2, which binds the Flag epitope on the MKK7 proteins. Activated JNK in the transfected cells was examined by using an antibody to phospho-JNK [JNK(P)]. The MKK7 (red) and phospho-JNK (green) were detected with secondary antibodies conjugated to Texas red and fluorescein, respectively. DNA was visualized by staining with DAPI (blue). The scale bar (white) represents 10 µm. (C) Regulation of MKK72 and NES-MKK72 by extracellular stimuli. COS-7 cells expressing MKK72 or NES-MKK72 were untreated (Control) or treated (30 min) with UV-C (80 J/m2) or IL-1 (10 ng/ml). The expression of MKK7 was examined by immunoblot (IB) analysis using the Flag-specific monoclonal antibody M2. MKK7 activity was measured in a coupled protein kinase assay (KA). The radioactivity incorporated into c-Jun was quantitated after SDS-PAGE by PhosphorImager analysis. MAPKK activity is presented as relative protein kinase activity. The levels of MAPKK activation caused by UV and IL-1 were 3.0- and 3.7-fold (MKK72) and 1.5- and 1.8-fold (NES-MKK72), respectively. (D) Activation of JNK1 by MKK7 and NES-MKK7. COS-7 cells were cotransfected with plasmids expressing HA-tagged JNK1 together with an empty vector (Control) or an expression vector encoding Flag-tagged MKK71, NES-MKK71, MKK72, or NES-MKK72. The expression of MKK7 and JNK1 was monitored by immunoblot (IB) analysis using antibodies to Flag and HA, respectively. JNK1 activity was measured by immune complex kinase assay (KA) using the substrate c-Jun. The radioactivity incorporated into c-Jun was quantitated after SDS-PAGE by PhosphorImager analysis. JNK activity is presented as relative protein kinase activity. The levels of JNK activation observed for MKK71, NES-MKK71, MKK72, and NES-MKK72 were 21-, 27-, 25-, and 31-fold, respectively.

	DISCUSSION

Top Abstract Introduction Materials and methods Results Discussion References

The genes that encode the JNK activators MKK4 and MKK7 are located on mouse chromosome 11. The MKK7 gene was mapped to mouse chromosome 11 (Fig. 2). It is interesting that the MKK4 gene is also located on this chromosome (81). The localization of both of the genes that encode JNK activators on the same mouse chromosome suggests that these genes might be linked. This is an interesting possibility because MKK4 is a candidate tumor suppressor gene that is mutated in certain tumors (73), suggesting that the MKK7 gene may also be a target of genetic alterations in disease processes.

The MKK7 gene encodes a group of protein kinases. The MKK7 gene includes 14 exons. Alternative splicing leads to the inclusion or exclusion of exons located in the 5' and 3' regions of the gene, resulting in the expression of a group of MKK7 isoforms that differ in their NH₂ and COOH termini (Fig. 3). These MKK7 protein kinase isoforms act as activators of JNK MAPK. Comparative studies demonstrate that the MKK7 isoforms differ in the extent of activation in response to different upstream components of the JNK signaling pathway (Fig. 7 to 9).

The MKK7 group of protein kinases are present in the cytoplasm and the nucleus. Studies of the ERK MAPK signaling pathway demonstrate that the ERK MAPKs are present in the cytoplasm of quiescent cells and that the ERKs accumulate in the nucleus following activation (9, 27, 66). Similarly, it has been reported by several groups that JNK accumulates in the nucleus following activation (8, 40, 51). In contrast to the activation-induced nuclear accumulation of the ERK MAPKs, the ERK activators MEK1 and MEK2 are cytoplasmic enzymes (1). A nuclear export sequence in the NH₂-terminal region of MEK1 appears to mediate rapid export out of the nucleus and, therefore, the accumulation of MEK1 in the cytoplasm (22, 23, 39). In contrast, we found that the JNK activator MKK7 was present in both the cytoplasm and the nucleus (Fig. 10). This nuclear location was observed for endogenous MKK7 (Fig. 10A) and for multiple MKK7 isoforms (Fig. 10B). Nuclear localization was also observed for the JNK activator MKK4. The mechanism that accounts for the nuclear location of MKK4 and MKK7 is unclear because the sequences of these protein kinases do not include an obvious nuclear localization sequence. It is possible that the absence of an NES may contribute to the localization of MKK4 and MKK7 in the nucleus. Thus, the subcellular location of the JNK activators MKK4 and MKK7 differs from that of the ERK activators MEK1 and MEK2.