INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

A number of genes encoding small structural RNAs are transcribed by RNA polymerase III in conjunction with various accessory factors that, in the simplest cases (tRNA genes) applicable to both yeast and metazoans, include the multisubunit TFIIIB and TFIIIC complexes (reviewed in references 12, 18, 44, and 45). For virus-associated (VA) RNA or mammalian tRNA genes, preinitiation complex (PIC) assembly involves promoter recognition (A and B boxes) by TFIIIC, followed by sequential recruitment of TFIIIB and RNA polymerase III (reviewed in reference 42). In yeast, TFIIIC induces the formation of a stable TFIIIB-promoter complex that is sufficient (even after TFIIIC dissociation) for RNA polymerase III recruitment and function (16), although a similar phenomenon has not been reported for metazoans.

The structural and functional analysis of RNA polymerase III accessory factors is most advanced in yeast. Yeast TFIIIC contains six polypeptides of 138, 131, 95, 91, 60, and 55 kDa (reviewed in references 1 and 27); yeast TFIIIB contains TATA-binding protein (TBP), a 70-kDa TFIIB-related factor (TFIIIB70/BRF), and a 90-kDa subunit (TFIIIB90/B") (reviewed in reference 19); and yeast RNA polymerase III contains 16 subunits ranging from 10 to 160 kDa (11). Photocross-linking studies have localized various of these components to specific regions of the tRNA gene, including localization of the yeast TFIIIC138 (yTFIIIC138) subunit to the B box region, the yTFIIIC95 subunit to the A box region, the yTFIIIC131 subunit to regions both upstream of the start site and between the A and B boxes, the yTFIIIB70 and yTFIIIB90 subunits to a region upstream of the start site, and the yRPC34, yRPC31, and yRPC82 subunits of RNA polymerase III to a region surrounding the transcription start site (2-4). Consistent with these results and a simple sequential recruitment model, yTFIIIC131 has been shown to interact directly with both yTFIIIB70 and yTFIIIB90, albeit not yet with yTFIIIC95 as predicted from the cross-linking analyses, and yTFIIIB70 has been shown to interact with yRPC34 (8, 20, 32, 43).

Corresponding studies of the human RNA polymerase III machinery have revealed a comparable complexity, although not all essential factors have been purified to homogeneity (reviewed in references 40 and 41). Human TFIIIC can be resolved chromatographically into a subcomplex (TFIIIC2) that has five subunits (of 220, 110, 102, 90, and 63 kDa) and exhibits limited (namely, B box) DNA-binding activity (21, 47) and a partially purified subcomplex (TFIIIC1) that enhances the binding of TFIIIC2 (39, 46). The more recent purification to near-homogeneity of a TFIIIC complex exhibiting the combined properties of TFIIIC2 and TFIIIC1 has revealed several candidate TFIIIC1 subunits and suggested that TFIIIC functions as a single, stable complex (42). Human TFIIIB has been shown to contain TBP and a subunit (human TFIIIB90 [hTFIIIB90]) related to yTFIIIB70 (29, 38), although more recent studies have identified additional candidate subunits related to yTFIIIB90 (14a, 36a). Consistent with a potentially greater complexity of the metazoan RNA polymerase III machinery, other factors affecting various aspects of transcription have also been reported (reviewed in references 40 and 42). Consistent with a phylogenetic conservation of the RNA polymerase III recruitment mechanism, hTFIIIB90 has been shown to interact with a human RNA polymerase III subunit (hRPC39) that is related to yRPC34, is present in a subcomplex with homologues (hRPC32 and hRPC62) of yRPC31 and yRPC82, and is specifically required for transcription initiation (41).

Despite the conservation of RNA polymerase III and TFIIIB subunits, as well as common core promoter elements (A and B boxes) for recognition by TFIIIC, the cognate cDNA clones for the two largest subunits of TFIIIC2 (including a B box contact subunit) failed to reveal any sequence relationship with the reported yeast TFIIIC subunits (23, 26, 34). To further understand the structure and function of human TFIIIC, including both similarities and dissimilarities with yeast TFIIIC, we have cloned cognate cDNAs and characterized two human TFIIIC2 subunits that have counterparts in yeast TFIIIC and that function through novel interactions in TFIIIB and RNA polymerase III recruitment.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Purification and cloning of hTFIIIC102 and hTFIIIC63. TFIIIC2 was purified as described previously (21), and component subunits were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Microsequencing analysis was performed on the 102 (hTFIIIC102)- and 63 (hTFIIIC63)-kDa polypeptides as described elsewhere (23). cDNA clones encoding hTFIIIC102 and hTFIIIC63 were obtained by screening human cDNA libraries (Namalwa, BJAB, and HeLa cell libraries) with several degenerate oligonucleotide probes corresponding to the internal peptide sequences of hTFIIIC102 and hTFIIIC63. DNA sequences were obtained by the dideoxynucleotide chain termination method (United States Biochemical).

Antigen and antiserum preparation. To produce His₁₀-tagged recombinant proteins for use as antigens, cDNAs encoding hTFIIIC102 (amino acids 207 to 711) and hTFIIIC63 (amino acids 1 to 519) were subcloned into pET-19b vectors. The resulting plasmids were introduced into Escherichia coli BL21(DE3)pLysS (35). After induction with isopropyl--D-thiogalactopyranoside (IPTG), recombinant proteins were purified by Ni²⁺-nitrilotriacetic acid (NTA)-agarose affinity chromatography followed by SDS-PAGE. For the preparation of antisera, New Zealand White rabbits were injected and boosted subsequently every 3 weeks with excised gel slices emulsified with Freund's adjuvant. Blood was collected 10 to 15 days after each boost. The antigens were cross-linked to CNBr-activated Sepharose 4B (Pharmacia) according to the manufacturer's instructions and were then used to purify the corresponding antisera as described previously (13).

Immunodepletion and immunoprecipitation of HeLa nuclear extracts with anti-hTFIIIC102 and anti-hTFIIIC63 antibodies. Antigen-purified anti-hTFIIIC102 and anti-hTFIIIC63 antibodies were first bound to protein A agarose (Oncogene Sciences) and then covalently cross-linked to the beads with dimethyl pimelimidate as described previously (13). As a control, the individual preimmune sera were processed the same way as immune sera. Antibody-coupled beads were incubated for 2 to 3 h at 4°C with HeLa nuclear extracts in buffer BC (20 mM Tris-HCl [pH 8], 20% glycerol, 0.2 mM EDTA, 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 mM dithiothreitol [DTT]) containing 500 mM KCl (BC500) and 0.1% Nonidet P-40 (NP-40). After centrifugation, the supernatant was dialyzed against BC100 and aliquots were frozen. The beads were washed extensively with the same buffer. The bound proteins were eluted with glycine (pH 2.5) and then neutralized with Tris-HCl (pH 8).

In vitro transcription assays. Transcription reactions for human tRNA and 5S genes and for adenovirus VAI genes were performed in a 25-µl reaction mixture with 250 ng of each supercoiled DNA template, 60 mM KCl, 6 mM MgCl₂, 2 mM DTT, 8% glycerol, 10 mM HEPES (pH 7.9), 0.6 mM (each) ATP, CTP and UTP, 0.025 mM GTP, and 2.5 µCi of [-³²P]GTP. Reactions were allowed to proceed for 1 h at 30°C before termination by the addition of 50 µl of stop solution (100 mM sodium acetate, 20 mM EDTA, 1% SDS, and 1 mg of yeast tRNA/ml). The mixtures were extracted with phenol-chloroform, precipitated with ethanol, and resolved on 8% polyacrylamide-7 M urea gels.

Protein-protein interaction assays. Purified recombinant proteins (0.5 to 2 µg) were bound to glutathione-Sepharose or M2 agarose beads and were incubated with 1 to 100 ng of the other purified recombinant protein in BC400-0.1% NP-40. The beads were washed extensively with BC400-0.1% NP-40. Residual proteins were eluted by boiling the beads in SDS gel sample buffer and were analyzed by Western blot analysis.

Assembly of the hTFIIIC102-hTFIIIC63-hTFIIIB90-TBP subcomplex. Sf9 cell lysates containing independently expressed FLAG-hTFIIIB90, hemagglutinin (HA)-hTFIIIC102, and His₁₀-hTFIIIC63 proteins and bacterial extracts containing glutathione S-transferase (GST)-TBP were mixed and incubated at 4°C in BC300-0.1% NP-40 for 2 to 3 h. The preassembled subcomplexes were first bound by Ni²⁺-NTA-agarose, then washed, and eluted in 1 M imidazole-BC300-0.1% NP-40. The eluted subcomplexes were immobilized on protein G-Sepharose coated with HA antibodies, washed, and eluted with BC300-0.1% NP-40-1 mg of HA peptides/ml. The HA peptide-eluted subcomplexes were then bound to glutathione-Sepharose, washed, and eluted with 100 mM glutathione-BC300-0.1% NP-40. The glutathione-eluted subcomplexes were finally immobilized on M2 agarose, washed, eluted by boiling in SDS gel sample buffer, and visualized by Western blot analysis.

Nucleotide sequence accession number. The GenBank accession no. of the hTFIIIC102 sequence is AF133123. The GenBank accession no. of the hTFIIIC63 sequence is AF133124.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Cloning and sequence analysis of hTFIIIC63 and hTFIIIC102. The purification of hTFIIIC2 has been described previously (21). The protein sequence information for four internal peptides of the 63-kDa subunit, designated hTFIIIC63, and four internal peptides of the 102-kDa subunit, designated hTFIIIC102, were obtained and used to clone cDNAs encoding all corresponding peptides. The deduced open reading frame of hTFIIIC63 cDNA encodes a polypeptide of 519 amino acids with a calculated molecular mass of 60 kDa (Fig. 1A). The deduced open reading frame of hTFIIIC102 cDNA encodes a protein of 886 amino acids with a predicted molecular mass of 101 kDa (Fig. 1B). A search of the National Center for Biotechnology Information nonredundant databases with the BLAST network service and the Genetics Computer Group GAP program servicer was performed to determine sequence similarities between hTFIIIC102 or hTFIIIC63 and other proteins. hTFIIIC63 shows significant sequence relationships to the TFIIIC95 (95) subunit of yeast TFIIIC (22% identity and 45% similarity) and to a hypothetical 50-kDa Caenorhabditis elegans protein (GenBank accession no. Z35603) (31% identity and 51% similarity). hTFIIIC102 shows 31% identity and 46% similarity to the TFIIIC131 (131) subunit of yeast TFIIIC, as well as 33% identity and 46% similarity to a hypothetical C. elegans protein (GenBank accession no. Z70783). In addition to the overall sequence similarity with yTFIIIC95, hTFIIIC63 shows conservation of a highly acidic region at the C terminus and a central helix-turn-helix motif (Fig. 1A). Like yTFIIIC131, hTFIIIC102 contains N-terminal acidic and basic regions, a central acidic region, a C-terminal helix-loop-helix (HLH) region, and, most notably, 11 tetratricopeptide repeats (TPRs) (Fig. 1C and D).

View larger version (141K): [in this window] [in a new window]   FIG. 1.   Predicted amino acid sequences and sequence alignments with yeast counterparts of the hTFIIIC102 and hTFIIIC63 subunits of the TFIIIC2 complex. The peptide sequences obtained by microsequence analyses are underlined. (A) Predicted amino acid sequence of hTFIIIC63 and alignment with yTFIIIC95 (31, 36). A highly acidic region at the C terminus and a central helix-turn-helix motif are double underlined. (B) Predicted amino acid sequence of hTFIIIC102 and alignment with yTFIIIC131 (28). (C) Schematic representation of hTFIIIC102 and yTFIIIC131 (28). Two basic regions are found at amino acid positions 23 to 34 and 130 to 147, and three acidic regions are found at amino acid positions 45 to 52, 92 to 114, and 363 to 374. The HLH motif is found at amino acid positions 552 to 600. (D) Consensus sequence of the TPR unit as defined by Sikorski et al. (33) and sequences of the 11 TPR units of hTFIIIC102. The four conserved residues that form "helix A" (residues 4, 7, 8, and 11), the three residues that form "helix B" (residues 20, 24, and 27), and the unique proline residue often found at position 32 (10, 14) are shown in boldfaced capital letters. Other residues that fit the TPR consensus sequence are shown in boldfaced lowercase letters. The following equivalences, based on the work of Marck et al. (28), are used: A, G, S, and V; E and D; K and R; I, L, M, and V; and L, F, Y, H, and W. Aspartate (D) residues, often found at positions 33 and 34 of TPR units, are underlined at those positions.

View larger version (35K): [in this window] [in a new window]   FIG. 2.   Identification of the cDNA-encoded proteins as the 102- and 63-kDa hTFIIIC2 subunits. The polypeptide components of a highly purified TFIIIC2 fraction were analyzed in a silver-stained gel (lane 1). Anti-hTFIIIC102 and anti-hTFIIIC63 antibodies were used to detect hTFIIIC102 and hTFIIIC63 in a highly purified TFIIIC2 fraction (lanes 2 and 4) and in purified reticulocyte lysate-expressed FLAG-hTFIIIC102 and baculovirus-expressed His10-hTFIIIC63 (lanes 3 and 5).

The cDNA-encoded 102- and 63-kDa proteins are both bona fide subunits of the TFIIIC2 complex and are involved in RNA polymerase III-mediated transcription. It has been established that human TFIIIC is required for the formation of stable PICs on 5S RNA, tRNA, and VA RNA genes (25). To determine whether the hTFIIIC102 and hTFIIIC63 cDNA-encoded proteins are components of the TFIIIC complex, antigen-purified anti-hTFIIIC102 and anti-hTFIIIC63 antisera, as well as control preimmune sera, were used to immunoprecipitate TFIIIC from HeLa nuclear extracts under stringent (0.5 M KCl-0.1% NP-40) conditions. As revealed by immunoblot analysis with antibodies to corresponding recombinant proteins (Fig. 3A), each immunoprecipitate, but neither preimmune serum precipitate, contained the 220-, 110-, 102-, 90-, and 63-kDa polypeptides that had been shown to copurify and correlate with the TFIIIC2 activity (21, 47). These results indicate that hTFIIIC102 and hTFIIIC63 are integral, tightly associated components of the TFIIIC2 complex. To determine the requirement for hTFIIIC102 and hTFIIIC63 in the transcription of 5S RNA, tRNA, and VA RNA genes, nuclear extract was depleted with either anti-hTFIIIC102 or anti-hTFIIIC63 antibodies and tested in a transcription assay. As shown in Fig. 3B, the high levels of transcription from 5S RNA, tRNA, and VAI RNA genes were reduced to undetectable levels after immunodepletion of either hTFIIIC102 (compare lane 5 with lane 1, lane 7 with lane 2, and lane 9 with lane 3) or hTFIIIC63 (compare lane 11 with lane 1, lane 13 with lane 2, and lane 15 with lane 3) but were unaffected by treatment with preimmune sera (lanes 4, 6, 8, 10, 12, and 14). Significantly, addition of an immunopurified TFIIIC complex containing both TFIIIC1 and TFIIIC2 (42), which were both required to restore transcription to anti-hTFIIIC110 immune serum-depleted extracts (34), restored to the depleted extracts transcription from the VAI template at levels similar to those observed with untreated extracts or with extracts treated with preimmune sera (Fig. 3C; compare lanes 4 and 5 with lanes 1 and 2, and lanes 8 and 9 with lanes 1 and 6). Thus, we conclude that hTFIIIC102 and hTFIIIC63, and associated polypeptides within TFIIIC, are necessary for RNA polymerase III-mediated transcription.

View larger version (40K): [in this window] [in a new window]   FIG. 3.   Immunoprecipitation of the TFIIIC2 complex and immunodepletion of TFIIIC transcription activity by anti-hTFIIIC102 and anti-hTFIIIC63 antibodies. (A) Immunoprecipitates from HeLa nuclear extracts treated with preimmune (P) and immune (I) anti-hTFIIIC102 (lanes 1 and 2) and anti-hTFIIIC63 (lanes 3 and 4) antibodies in BC500-0.1% NP-40 were subjected to Western blot analysis. The polypeptides were detected by a mixture of antibodies against hTFIIIC220, -110, -102, -90, and -63. The amounts of individual antibodies were adjusted so that the intensities of the hTFIIIC220, -110, -102, -90, and -63 immunoreactive bands were similar. The extra band between the 63- and 90-kDa polypeptides in lanes 2 and 4 reflects cross-reactivity with the anti-hTFIIIC110 antibodies and appears to represent a proteolytic breakdown product of hTFIIIC110 that was not consistently observed. (B) Nuclear extracts treated with preimmune (P) and immune (I) anti-hTFIIIC102 (lanes 4 to 9) and anti-hTFIIIC63 (lanes 10 to 15) antibodies were used for in vitro transcription assays with 5S RNA (lanes 1, 4, 5, 10, and 11), VAI RNA (lanes 2, 6, 7, 12, and 13), and tRNA (lanes 1, 8, 9, 14, and 15) templates. (C) Either 1 µl (lanes 4 and 8) or 2 µl (lanes 5 and 9) of an immunopurified TFIIIC was added to nuclear extracts depleted with immune anti-hTFIIIC102 (lanes 4 and 5) or with anti-hTFIIIC63 (lanes 8 and 9) and tested for transcription with the VAI RNA template.

Interaction of hTFIIIC63 and hTFIIIC102 with each other and with hTFIIIB90 and TBP. In yeast, it has been shown that PIC assembly on tRNA genes involves promoter recognition by TFIIIC followed by TFIIIB recruitment through protein-protein interactions. yTFIIIC95 and yTFIIIC131 are positioned, respectively, at and on both sides of the A box (2, 3), and yTFIIIC131 recruits TFIIIB through interactions with the TBP-interacting yTFIIIB70 subunit (8, 20) and with yTFIIIB90 (32). Since hTFIIIC102, hTFIIIC63, and hTFIIIB90 are homologues of yTFIIIC131, yTFIIIC95, and yTFIIIB70, respectively, we used purified recombinant proteins to test the potential interactions among hTFIIIC102, hTFIIIC63, hTFIIIB90, and TBP under stringent (0.4 M KCl-0.1% NP-40) conditions. As shown in Fig. 4A, purified baculovirus-expressed hTFIIIC102 (detected by anti-hTFIIIC102 antibodies) was bound to M2 agarose-immobilized FLAG-tagged hTFIIIC63 but not to M2 agarose alone. Similarly, purified baculovirus-expressed hTFIIIC102 and hTFIIIC63 (detected by anti-hTFIIIC102 and anti-hTFIIIC63 antibodies) were bound to M2 agarose-immobilized FLAG-TFIIIB90 but not to M2 agarose (Fig. 4B). Purified hTFIIIC102 and hTFIIIC63 showed similar interactions with glutathione-Sepharose-immobilized GST-TBP, but not with glutathione-Sepharose-immobilized GST alone (Fig. 4B). These results indicate that hTFIIIC102 and hTFIIIC63 associate tightly with each other and with hTFIIIB90 and TBP in the absence of other associated subunits.

View larger version (35K): [in this window] [in a new window]   FIG. 4.   Interactions of hTFIIIC102 with hTFIIIC63, hTFIIIB90, and TBP, and interactions of hTFIIIC63 with hTFIIIB90 and TBP. Input samples contained 10% of the amounts used for the interactions. (A) Purified baculovirus-expressed HA-hTFIIIC102 was incubated with M2 agarose or M2 agarose containing bound FLAG-hTFIIIC63 (M2-fIIIC63), and samples were washed and eluted as described in Materials and Methods. HA-hTFIIIC102 in input and eluted fractions was detected by immunoblotting with antisera against hTFIIIC102. (B) Purified baculovirus-expressed His10-hTFIIIC63 (top panel) or HA-hTFIIIC102 (bottom panel) was incubated with M2 agarose or M2 agarose-immobilized FLAG-hTFIIIB90 (M2-fB90) and with glutathione-Sepharose-immobilized GST or GST-TBP, and samples were washed and eluted as described in Materials and Methods. His10-hTFIIIC63 and HA-hTFIIIC102 were detected in input and eluted fractions by immunoblotting with anti-hTFIIIC63 and anti-hTFIIIC102 antibodies, respectively. (C) Sf9 cell extracts containing expressed His10-hTFIIIC63 (upper panel) or FLAG-hTFIIIB90 (lower panel) were incubated with glutathione-Sepharose-immobilized GST or with GST-truncated-hTFIIIC102 proteins (A, amino acids 1 to 148; B, amino acids 1 to 214; C, amino acids 207 to 507; D, amino acids 204 to 325; E, amino acids 419 to 711; F, amino acids 326 to 420; G, amino acids 419 to 531; and H, amino acids 527 to 724) in BC400-0.1% NP-40. After extensive washing of the beads with the same buffer, bound proteins were eluted by boiling in SDS sample buffer and analyzed by immunoblotting with antisera against hTFIIIC63 and hTFIIIB90. The amounts of GST-truncated-hTFIIIC102 proteins A through H and GST proteins were normalized by SDS-PAGE with Coomassie blue staining.

hTFIIIC102, hTFIIIC63, hTFIIIB90, and TBP form a stable subcomplex. The demonstrated interaction of hTFIIIC102 with hTFIIIB90 is similar to that reported for the yeast counterparts (8, 20), whereas the interactions of hTFIIIC63 with hTFIIIB90 and TBP and the interactions of hTFIIIC102 with hTFIIIC63 and with TBP represent novel observations that further strengthen the PIC assembly model. To test the prediction from these studies that hTFIIIC63, hTFIIIC102, hTFIIIB90, and TBP may form a subcomplex, Sf9 cell extracts containing independently expressed His₁₀-hTFIIIC63, FLAG-hTFIIIB90, and HA-hTFIIIC102 proteins and bacterial extracts containing GST-TBP were mixed to form a presumptive subcomplex of four subunits. The mixture was then subjected to four successive affinity purification steps (with Ni²⁺-NTA-agarose, HA antibodies covalently linked to protein G-Sepharose, glutathione-Sepharose, and M2 agarose), each specific for a distinct recombinant protein, and the resulting preparation was analyzed by immunoblot analysis. The results in Fig. 5 show that the final immunopurified preparation contains hTFIIIC102, hTFIIIC63, hTFIIIB90, and TBP, indicating that these components can be assembled into a subcomplex that is sufficiently stable to survive four independent affinity purification methods at 300 mM KCl-0.1% NP-40. The results of gel filtration analyses (data not shown) are also consistent with the formation of a stable subcomplex.

View larger version (25K): [in this window] [in a new window]   FIG. 5.   hTFIIIC102, hTFIIIC63, hTFIIIB90, and TBP form a subcomplex in vitro. The subcomplex formed from the baculovirus-expressed His10-hTFIIIC63, HA-hTFIIIC102, and FLAG-hTFIIIB90 and bacterially expressed GST-TBP was purified by successive affinity chromatography on Ni2+-NTA-agarose, HA antibodies linked to protein G-Sepharose, glutathione-Sepharose, and then M2 agarose. The preparative subcomplex was analyzed by immunoblotting with a mixture of antibodies against hTFIIIC102, hTFIIIC63, hTFIIIB90, and TBP. The relative amounts of the individual antibodies were different from the amounts used in Fig. 3A and 6B, such that the different intensities of the hTFIIIC102 and hTFIIIC63 immunoreactive bands do not necessarily reflect different stoichiometric amounts of these components.

Interactions between TFIIIC, TFIIIB, and RNA polymerase III. Yeast RNA polymerase III is recruited to the TFIIIB-TFIIIC promoter complex through interactions with TFIIIB (16), and this involves interactions of yTFIIIB70 with an RNA polymerase III subunit (yRPC34) that is part of a dissociable three-subunit complex (6, 43). A similar mechanism appears to operate in the human system, since binding of TFIIIB is dependent on prior binding of TFIIIC (5, 25) and since hTFIIIB90 and human TBP interact with an RNA polymerase III subunit (hRPC39) that is homologous to yRPC34 and part of an initiation-specific subcomplex containing hRPC62 and hRPC32 in addition to hRPC39 (41). Given the novel human TFIIIC and TFIIIB subunit interactions described above, as well as the availability of recombinant human RNA polymerase III subunits RPC62, RPC39, and RPC32, we tested whether any of the latter subunits could interact with hTFIIIC63 or hTFIIIC102. The GST fusion protein binding assays in Fig. 6A show that purified hTFIIIC63 interacts with GST-hRPC62 (lane 5) but not with equivalent amounts of GST-hRPC39 (lane 4), GST-hRPC32 (lane 3), or GST alone (lane 2). None of these RNA polymerase III subunits showed stable interactions with purified hTFIIIC102 (Fig. 6A). We further tested whether these interactions occur in the context of the TFIIIC complex. In support of this idea, an immunopurified TFIIIC complex, including all five TFIIIC2 subunits, bound to GST-hRPC62 but not to GST alone (Fig. 6B).

View larger version (30K): [in this window] [in a new window]   FIG. 6.   Interactions of hTFIIIC102 and hTFIIIC63 with RNA polymerase III subunits hRPC32, hRPC39, and hRPC62. Input samples contained 10% of the amounts used for the interactions. (A) Purified baculovirus-expressed His10-hTFIIIC63 (top panel) or HA-hTFIIIC102 (bottom panel) was incubated with glutathione-Sepharose-immobilized GST-hRPC32 (lane 3), GST-hRPC39 (lane 4), GST-hRPC62 (lane 5), and GST (lane 2) proteins in BC150-0.1% NP-40. After extensive washes with the same buffer, the beads were boiled in SDS sample buffer. His10-hTFIIIC63 and HA-hTFIIIC102 in input and eluted fractions were detected on immunoblots by antisera against hTFIIIC63 and hTFIIIC102, respectively. The amounts of GST-hRPC32, GST-hRPC39, GST-hRPC62, and GST proteins in the inputs were normalized by SDS-PAGE with Coomassie blue staining. (B) Beads containing glutathione-Sepharose-immobilized GST-hRPC62 or GST were incubated with an immunopurified TFIIIC in BC150-0.1% NP-40. After extensive washes with the same buffer, the beads were boiled in SDS sample buffer. hTFIIIC220, -110, -102, -90, and -63 in the SDS eluates were detected by immunoblotting with a mixture of antisera against hTFIIIC220, -110, -102, -90, and -63. The amounts of individual antibodies were adjusted so that the intensities of the hTFIIIC220, -110, -102, -90, and -63 immunoreactive bands were similar.

View larger version (12K): [in this window] [in a new window]   FIG. 7.   Interactions between TFIIIB, TFIIIC, and RNA polymerase III. Input samples contained 10% of the amounts used for the interactions. (A) M2 agarose or M2 agarose-immobilized FLAG-hTFIIIB90 (M2-fIIIB90) was incubated with an immunopurified TFIIIC and washed with BC400-0.1% NP-40. Bound proteins were eluted with SDS buffer and analyzed by immunoblotting with antisera against hTFIIIC63. (B) Ni2+-NTA-agarose or Ni2+-NTA-agarose-immobilized His10-hTFIIIC63 and anti-HA agarose or anti-HA agarose-immobilized hTFIIIC102 were incubated with a purified core FLAG-hTFIIIB90-GST-TBP subcomplex. The latter subcomplex was isolated by successive affinity chromatography of a mixture of baculovirus-expressed FLAG-hTFIIIB90 and bacterially expressed GST-TBP on glutathione-Sepharose and M2 agarose, with BC400-0.1% NP-40 washes prior to elution (38). Bound proteins were eluted in the SDS buffer and analyzed by immunoblotting with antisera against hTFIIIB90. (C) Ni2+-NTA-agarose or Ni2+-NTA-agarose-immobilized His10-hTFIIIC63 was incubated with an immunopurified RNA polymerase III and washed with BC150-0.1% NP-40. Bound proteins were eluted in the SDS buffer and analyzed by immunoblotting with antisera against hRPC82 and hRPC53.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

TFIIIC plays a primary role in promoter recognition and formation of stable PICs on a subset of genes transcribed by RNA polymerase III. Toward a more detailed analysis of the central role of human TFIIIC in these processes, we report the cloning and characterization of cDNAs encoding two subunits (hTFIIIC102 and hTFIIIC63) of the TFIIIC2 subcomplex that directly binds to tRNA and VA RNA promoters. These cDNAs have been used to investigate the primary structure, phylogenetic conservation, and mechanism of action of TFIIIC. In contrast to previously reported TFIIIC2 subunits with no yeast counterparts, hTFIIIC102 and hTFIIIC63 have yeast homologues and show overlapping but more-extensive interactions than those reported for their yeast counterparts.

Structure and evolutionary conservation of hTFIIIC102 and hTFIIIC63. Given that yeast TFIIIC and human TFIIIC are multisubunit complexes and recognize similar promoter sequences (A and B boxes) in various class III genes, it was surprising to find that human TFIIIC220 (TFIIIC) and yeast TFIIIC138 (138), which both make B box contacts, show no significant sequence similarity (23, 26) and, further, that human TFIIIC110 (TFIIIC) also shows no significant sequence similarity to any of the five cloned subunits (138, 131, 95, 91, and 55 kDa) of yeast TFIIIC (34). However, since most or all of the TFIIIB (14a, 29, 36a, 38) and RNA polymerase III (reviewed in references 40, 41, and 42a) subunits are conserved from yeast to human, it was anticipated that human TFIIIC would contain at least some subunits with sequence relationships to yeast TFIIIC (notably those interacting with conserved TFIIIB subunits). Consistent with this notion, hTFIIIC63 and hTFIIIC102 have significant sequence and functional relationships, respectively, to the TFIIIC95 and TFIIIC131 subunits of yeast TFIIIC. hTFIIIC63 preserves, minimally, the highly acidic region and helix-turn-helix motif of yTFIIIC95, and hTFIIIC102 preserves the charged regions, TPRs, and HLH domain structures of yTFIIIC131.

Interactions of human TFIIIC, TFIIIB, and RNA polymerase III in relation to PIC assembly. As detailed in the introduction, DNA binding and photoaffinity cross-linking studies revealed approximate topological positions of yeast TFIIIC, TFIIIB, and RNA polymerase subunits on the promoter and suggested interactions that were confirmed in part by direct protein-protein interactions and genetic interaction analyses. Consistent with the simple sequential recruitment model, the genetic interaction studies reported (i) interactions of yTFIIIC131 with yTFIIIB70 and yTFIIIB90, which are situated in the most distal occupied region of the promoter in the PIC (however, the expected physical interaction of yTFIIIC95, which is localized within the PIC in the A box region, with yTFIIIC131, part of which is situated upstream of the start site in the PIC, has not yet been demonstrated), and (ii) interaction of yTFIIIB70 with yRPC34, which also is localized to an upstream region of the A box in the PIC. In agreement with the sequence conservation of all these components from yeast to human, including the relationships of the newly described hTFIIIC63 and hTFIIIC102 to yTFIIIC95 and yTFIIIC131, respectively, we have demonstrated that hTFIIIC102 can interact with hTFIIIB90 (homologue of yTFIIIB70) and, in a previous report (41), that hTFIIIB90 can interact with hRPC39 (homologue of yRPC34). Significantly, however, we have also described novel interactions of human TFIIIC components not previously described for the homologous yeast components. These include the important interaction between hTFIIIC102 and hTFIIIC63, which documents a direct link between the TFIIIB-interacting and DNA-interacting subunits of TFIIIC, as well as interactions between hTFIIIB90 and hTFIIIC63, interactions between TBP and both hTFIIIC102 and hTFIIIC63, and interactions between hRPC62 and hTFIIIC63 (summarized in Fig. 8). The latter results suggest that additional TFIIIB-TFIIIC and TFIIIC-RNA polymerase III contacts may facililate, perhaps via concerted interactions, both TFIIIB and RNA polymerase III recruitment. Alternatively, these interactions may also be involved in the function of the PIC components during initiation, elongation, termination, or reinitiation steps. The hTFIIIC63-hRPC62 interaction is particularly interesting because the corresponding yeast components appear to be localized to a common position in the PIC (4, 9) and because hRPC62 is present, with hRPC32 and hRPC39, in a subcomplex required specifically for transcription initiation by human RNA polymerase III (41). These observations also suggest, contrary to the prevailing yeast model (18), that human TFIIIC may have functions in addition to TFIIIB recruitment, and they are reminiscent of recent indications that human TFIIIC has a (presumably distinct) role in transcription termination and reinitiation by RNA polymerase III (42).

View larger version (19K): [in this window] [in a new window]   FIG. 8.   An integrated view of the interactions detected between subunits of human TFIIIB, TFIIIC, and RNA polymerase III. The observed protein-protein interactions are indicated by double-headed arrows. The A box and B box of the VAI/tRNA gene promoter are shown as hatched and solid rectangles, respectively. Hatched symbols, subcomplex of initiation-specific RNA polymerase III subunits; shaded symbols, TFIIIB subunits; open symbols, TFIIIC2 subunits. Individual subunits involved in interactions between TFIIIC2 and TFIIIC1 or between core RNA polymerase III and the three-subunit subcomplex are unknown. The positioning of the hTFIIIC220 subunit is based on cross-linking studies (21), whereas the positioning of the hTFIIIC63 and hTFIIIC102 subunits, as well as that of the TFIIIB subunits, is based on studies of the cognate yeast components.

Role of the TPRs in TFIIIC function. Tandem arrays of the TPR have been found in a wide variety of eukaryotic proteins and are thought to mediate protein-protein interactions (reviewed in reference 24). In relation to the present study, yTFIIIC131 (homologue of hTFIIIC102) contains three clusters of TPR motifs (28) in N-terminal (TPR1 through -5), central (TPR6 through -9), and C-terminal (TPR10 through -11) regions. yTFIIIC131 fragments containing at least two repeats were shown to interact independently with yTFIIIB70 in vitro (20), whereas a fragment containing the N-terminal region and the adjoining TPR1 was sufficient for in vivo interactions with yTFIIIB70 (8). The failure of the in vivo (yeast two-hybrid) assays to detect yTFIIIB70 interactions with yTFIIIC131 lacking only the small N-terminal regions may have reflected autoinhibitory effects (within yTFIIIC131) that are normally reversed by conformational changes dependent on multiple interactions between full-length yTFIIIC131 and yTFIIIB70 (8).