INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

Most eukaryotic chromosome ends are maintained by a ribonucleoprotein (RNP) complex called telomerase. Telomerase is a reverse transcriptase that uses an integral RNA component to catalyze the addition of telomeric repeats to the 3' end of single-stranded telomeric DNA (8).

In many organisms, the telomerase complex is a large (750- to 1,000-kDa) RNP containing an integral RNA, a reverse transcriptase protein subunit, and several associated proteins. The telomerase RNA component provides a template for telomere DNA synthesis, and its essential role in telomerase activity, telomere length maintenance, and chromosome stability has been demonstrated in ciliates, yeast, and mice (2, 14-16, 29, 39, 42). The telomerase reverse transcriptase (TERT) was first identified in the yeasts Saccharomyces cerevisiae (EST2) and Schizosaccharomyces pombe (trt1⁺) and the ciliate Euplotes aediculatus (p123) (32, 36) and subsequently in humans (hTERT) (12, 25, 34, 36, 38). Mutations of conserved amino acids within the reverse transcriptase domain of S. cerevisiae Est2 and in human TERT result in the loss of telomerase activity (6, 12, 32, 38, 46). In rabbit reticulocyte lysates, human telomerase activity is reconstituted by the addition of human TERT (hTERT) and the telomerase RNA (1, 46).

In addition to the presumed core telomerase components, consisting of the telomerase RNA and TERT, several proteins associated with telomerase activity have also been identified. In humans, the "foldosome" proteins hsp90 and p23 and three telomerase RNA binding proteins, dyskerin, L22, and hStau, are each associated with telomerase activity in cell extracts (18, 28, 35). In S. cerevisiae, the Sm protein is necessary for the stability of the yeast telomerase RNA (44), while three other proteins, Est1, Est3, and Cdc13, are dispensable for telomerase activity but are required for telomere length maintenance (30, 33, 45). In the ciliate Tetrahymena thermophila, two proteins that copurify with telomerase, p80 and p95 (4, 7, 10), bind to telomerase RNA and telomeric DNA, although their precise role in the Tetrahymena telomerase complex is not yet clear (4, 7).

The mammalian homolog of p80, TEP1, is associated with telomerase activity in human, mouse, and rat immortalized-cell extracts (11, 37). The amino-terminal 900 amino acids of TEP1, which contain the region homologous to Tetrahymena p80, also interacted with telomerase RNA in an in vivo RNA-protein interaction assay (11). Despite its association with telomerase components, the role of TEP1 in telomerase function is unknown. Recently, TEP1 has also been identified as a component of a large cytoplasmic RNP termed the vault particle (24). The genetic characterization of these proteins is critical to our understanding of the complexity, composition, and regulation of telomerase in vivo. We utilized homologous recombination to disrupt the first mammalian telomerase-associated protein to be identified, mTep1, in mice and embryonic stem (ES) cells and analyzed the effects on telomerase activity and telomere length maintenance.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Construction of a murine Tep1 targeting vector. To isolate murine Tep1 genomic fragments, a mouse Tep1 BACMID genomic DNA clone was obtained from Genome Systems Inc. (St. Louis, Mo.) and a full-length mTep1 cDNA probe was used. Two genomic KpnI/SphI fragments (approximately 6.3 and 2.3 kb) of the mTep1 BACMID DNA hybridized to a cDNA probe spanning a region containing the first third of the mTep1 open reading frame. These fragments were subcloned into pSPORT (Stratagene). A targeted construct was designed to replace four exons of mTep1 with the neomycin resistance gene. In brief, PCR primers (5'-CTCGAGGTTCGTAGGGTCAATGGTGTGTC-3' [sense] and 5'-GTCGACATTTCTGTGTTCAAGACAAATCAG-3' [antisense]) were used to amplify a 3.7-kb long-arm fragment from the 6.3-kb mTep1 genomic clone using the Expand long template PCR system (Boehringer Mannheim). Similarly, an ~770-bp short arm was amplified from the 2.3-kb mTep1 genomic clone using the PCR primers 5'-TCTAGATAGGTGGCGTTGATCGGTGATCG-3' (sense) and 5'-GCGGCGGCAACCTTTTGAAGAACAACCAATG-3' (antisense).

Targeted disruption of the mTep1 gene in ES cells. The mTep1 targeting vector was linearized with NotI at the short arm and electroporated into E14 ES cells (derived from the R129J strain). After G418 selection (0.3 mg/ml), homologous recombinants were identified by PCR and confirmed by Southern blot analysis, following published protocols (9). Primer TEP1-1, which is specific for the deleted portion of mTep1, was used to detect the wild-type allele and primer TEP1-2 was used to detect the mutant allele, while primer TEP1-3 was used to detect both the wild-type and mutant alleles of mTep1. PCR amplification was carried out with 30 cycles of 94°C for 1 min, 62°C for 1 min, and 72°C for 1 min. The sequences of the above primers are as follows: TEP1-1, 5'-CCAGCAGTATGAGGGTCGTCAGTGG-3'; TEP1-2, 5'-GCTAAAGCGCATGCTCCAGAC-3'; and TEP1-3, 5'-CACATGCCTGTCTGGTTCTGTGGAG-3'.

Generation of mTep1-deficient mice and ES cells. Chimeric mice were produced by microinjection of three independent mTep1^+/ ES cell clones into E3.5 C57BL/6J blastocysts and transferred to ICR pseudopregnant foster mothers. Chimeric males were mated with C57BL/6J females (Jackson Laboratory). Germ line transmission of the mutant allele was confirmed by PCR and Southern blot analysis of tail DNA from mice with an agouti coat color. The mating strategy for the mTep1^/ mice was the same as that used for the generation of the telomerase RNA-deficient mice (2). In brief, the first generation of mTep1^/ homozgyous animals were referred to as G1, and the subsequent generations (obtained by mating two mTep1^/ animals of an equivalent generation but derived from a different founder) were G2, G3, etc. mTep1^/ ES cell clones were generated from G418-resistant mTep1^+/ ES cell clones by culturing with an increased G418 concentration (4 mg/ml). ES cell culture was carried out as previously described (9).

Cell lysate preparation and telomerase assays. S-100 extracts from cultured cells and freshly dissected mouse tissues were prepared as described previously (41). Cells lysed in a buffer containing 0.5% CHAPS {3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate} were prepared as described elsewhere (26) (Intergen, Inc.). Telomerase activity was assayed using the telomeric repeat amplification protocol (TRAP) assay following the manufacturer's instructions (26) (Intergen, Inc.). The standard elongation assay for telomerase activity was performed on S-100 extracts after fractionation on DEAE-agarose columns as described previously (5, 40).

Immunoprecipitation analysis of mTep1. Immunoprecipitation analysis using an affinity-purified anti-TEP1 polyclonal rabbit antibody was performed as described previously (12). The specificity of the TEP1 polyclonal antibody was confirmed by Western analysis using cell extracts from human 293 cells overexpressing recombinant hTEP1 and using a glutathione transferase-mTEP1 recombinant purified fusion protein (12).

Telomere length measurements by FISH. The average telomere fluorescence at chromosome ends in splenocytes, thymocytes, and ES cells was measured by using fluorescence in situ hybridization (FISH) combined with flow cytometry (Flow-FISH) (43), with minor modifications. A telomere-specific fluorescein isothiocyanate-conjugated (CCCTAA)₃ peptide nucleic acid probe (0.3 µg/ml) (Perseptive Biosystems) was employed. Telomere fluorescence is expressed as molecules of equivalent soluble fluorochrome (MESF) (13).

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Targeted disruption of mTep1 genes. The mTep1 targeting strategy was designed to disrupt a region of mTep1 that is required for binding to the murine telomerase RNA in a yeast three-hybrid interaction assay (11) (Fig. 1A). Three separate heterozygote mTep1 (mTep1^+/) ES cell clones were used to generate mTep1^+/ founder mice (data not shown). One of the mTep1^+/ ES cell clones was used to generate three nullizygous mTep1 (mTep1^/) clones. Different mTep1^+/ founders were bred together to generate mTep1^/ mice (Fig. 1B). The mTep1^/ mice were designated as generation 1 (G1), and the progeny of G1 cousins were defined as G2, et cetera, as described previously (2). Seven generations (to G7) of mTep1^/ mice were fertile and showed no obvious phenotypic defects compared to wild-type mice. Northern analysis of poly(A)⁺ mRNA from several mTep1^/ mouse tissues showed no detectable transcripts upon hybridization to a full-length mTep1 cDNA probe (data not shown). Analysis of cell extracts prepared from mTep1^/ G1 MEFs with an affinity-purified anti-TEP1 rabbit polyclonal antiserum (spf2) revealed no detectable mTep1 protein in lysates or anti-TEP1 immunoprecipitation from mTep1-deficient mouse tissues (Fig. 1C and data not shown).

View larger version (28K): [in this window] [in a new window]   FIG. 1.   Disruption of the murine Tep1 gene in ES cells and mice. (A) Schematic representation of homologous recombination of the targeting vector with the endogenous locus. Insertion of a new Spe1 site into the targeted locus allows the targeted and wild-type alleles to be distinguished by Southern blot analysis with the indicated 3' flanking probe. S, SpeI; N, Not1. (B) Detection of targeted and wild-type mTep1 alleles by Southern blot analysis of DNA from G1 mice. DNA was digested with SpeI and hybridized with the probe shown in panel A. The 5.1-kb SpeI fragment corresponding to the wild-type allele is decreased to 1.6 kb upon disruption of the locus by integration of the Neor gene. (C) Immunoblot of MEF extracts probed with an anti-TEP1 antibody (12). The position of the mTep1 protein is indicated. The first lane contains human 293 cell lysate transfected with a recombinant hTEP1 plasmid (293-hTEP1). The protein species below the 85-kDa protein marker in each immunoprecipitate is not specific to anti-TEP1 antibody.

mTep1 is not essential for telomerase catalysis in vivo. To determine the role of mTep1 in telomerase catalysis, we examined mTep1-deficient ES cells, MEF cultures derived from G1 mTep1^/ embryos, and several tissues from mTep1^/ mice (up to G3) for telomerase activity. In both the conventional telomerase elongation assay and the PCR-based telomerase assay (TRAP) (26), all three independently derived mTep1^/ ES cell clones possessed levels of telomerase activity similar to those in the mTep1^+/ clone and the parental line (data not shown). We also tested whether telomerase activity was altered in tissues from mTep1^/ mice. Telomerase activity was not significantly altered in testes, liver, kidney, lung, or thymus tissue from mTep1^/ mice compared to wild-type mouse tissues (Fig. 2A and Table 1). Subsequent analysis of several tissues that normally lack telomerase activity, including brain, skin, heart, and spleen (2, 41), revealed no reactivation of telomerase activity in these tissues from mTep1^/ mice (Table 1 and data not shown). In MEFs derived from G1 mTep1^/ embryos, there was also no significant change in telomerase activity compared to that in the mTep1^+/ and wild-type MEFs (Table 1 and data not shown). In the conventional telomerase assay, we did not detect any changes in telomerase activity levels in liver or testis extracts from mTep1^/ mice (Fig. 2B).

View larger version (51K): [in this window] [in a new window]   FIG. 2.   Telomerase activity of mTep1-deficient tissues. (A) TRAP was performed for 25 cycles on testis tissue extracts of the indicated genotype. R, RNase A treatment of the sample. An internal PCR standard for TRAP is shown at bottom right. The amounts of testis extract used was as follows: 4 µg of extract plus RNase A (wild-type testis), or 4, 2, 1, and 0.5 µg of testis extract. (B) The conventional telomerase elongation assay was performed on murine liver and testes. A telomerase-positive human cell extract (Raji) is shown as a positive control. The arrow indicates the position of telomerase elongation products from mouse tissue extract, which were sensitive to RNase A treatment (R). The amount of extract used for each sample was 15 or 7.5 µg.

The role of mTep1 in telomere length regulation in vivo. Mice and ES cells disrupted in the telomerase RNA component lack telomerase activity and undergo telomere attrition in vivo (2, 16, 29, 39). In S. cerevisiae, EST1, EST3, and CDC13 are not required for telomerase activity (31); however, mutation or disruption of these genes results in progressive telomere loss (30, 31). To determine whether loss of mTep1 could be essential for telomere length maintenance, we analyzed telomere length in mTep1-deficient mice and ES cells.

+/

/

View larger version (26K): [in this window] [in a new window]   FIG. 3.   Relative telomere lengths in ES cells and in splenocytes and thymocytes derived from mouse tissues, determined by Flow-FISH. (A) Average telomere fluorescence in early (p1) and later (p10 and p20) passages of ES cells, including wild type, mTep1+/, and three mTep1/ clones. Each MESF value represents the results for one individual ES clone at the indicated passage; hence, there are no error bars. (B) Average telomere fluorescence in splenocytes and thymocytes derived from early and late generations (G1 through G7) of mTep1/ mice. In each set, data were pooled from at least five mice (error bars represent standard deviations).

View larger version (38K): [in this window] [in a new window]   FIG. 4.   FISH analysis of metaphase preparations of primary MEFs derived from G1 mTep1+/+ (A) or G5 mTep1/ embryos (B). No significant changes in telomere signal intensity or chromosomal aberrations were detected in the G5 mTep1/ embryos.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

In mammals, the first genetic evidence for the essential role of telomerase activity in telomere length maintenance was elegantly demonstrated in mice lacking the telomerase RNA component (2, 14-16, 29, 39, 42). In this study, we have used a genetic approach to examine the role of the first identified mammalian telomerase-associated protein, mTep1, in telomerase catalysis and telomere length regulation in vivo.

Despite the fact that TEP1 is associated with the telomerase RNA and the telomerase catalytic subunit TERT in cell extracts from immortalized human, mouse, and rat cells, mTep1-deficient mice show no significant alteration in telomerase activity or telomere length. These results indicate that mTep1 is not essential for the catalytic activity of the telomerase complex, at least as measured by the in vitro elongation assay, and that as observed when deleted alone, mTep1 is not required for telomere length maintenance. These results are consistent with our previous finding from an in vitro assay that the reconstitution of human telomerase activity does not require the addition of hTEP1 (1).

Several possibilities could account for the absence of a telomerase-associated phenotype in mTep1^/ mice. Since mTep1 is associated with telomerase activity in immortalized cells, it is possible that it plays a specific role in the function of telomerase in immortalized cells but not in normal cells (11). Another possibility is that if TEP1 is associated with only a fraction of the total telomerase activity in vivo, its disruption might have no overt phenotypic consequences. A substoichiometric association of TEP1 with telomerase activity is suggested by the observation that antibodies against TEP1, hStau, and L22 are each able to immunoprecipitate telomerase activity, but not each other, from human immortalized cell extracts (28). Alternatively, other telomerase RNA binding proteins may share a redundant role with mTep1 in telomerase function. In Tetrahymena, both p80 and p95 synergistically bind to the telomerase RNA (4, 7). A mammalian homolog of p95 has not yet been identified. In mammals, two additional telomerase RNA binding proteins, L22 and hStau, were cloned using a yeast three-hybrid screen (28). These proteins are each associated with telomerase activity and hTERT, and yet there is no evidence of a direct association with each other or with TEP1 (28). Whether separate telomerase complexes containing distinct telomerase RNA binding proteins share overlapping functions in vivo has yet to be determined. Indirect immunofluorescence analysis of TEP1 and TERT will be instrumental in demonstrating whether TEP1 indeed colocalizes with a subset of hTERT in intact cells.

Not all telomerase-associated proteins appear to be restricted to the telomerase complex. At least some fraction of human telomerase activity is associated with p23 and Hsp90, two components of the ubiquitous foldosome which facilitate assembly of the active telomerase complex in rabbit reticulocyte lysates (18). TEP1 is expressed in many tissues, including those that lack telomerase activity, suggesting that TEP1 may be important for other cellular functions. In support of this notion, TEP1 has also recently been discovered to be an integral component of a large cytoplasmic (13-MDa) RNP complex referred to as the vault particle (24). Vault particles have been isolated from a number of organisms and appear to be ubiquitous among eukaryotes (19). Although the function of the vault particle is unknown, several recent reports have implicated the particle in intracellular transport (3, 17, 20, 27). Purified vaults display an eight-fold, barrel-like symmetry and contain at least three components in addition to TEP1, including the 104-kDa major vault protein MVP (21), a 193-kDa poly(ADP-ribosyl) polymerase called VPARP (23), and a small RNA called vRNA (20, 22). TEP1, but not telomerase activity, copurifies with the vault particle, and several vRNAs specifically interact with TEP1 in the yeast RNA-protein interaction assay (24). The so-called subunit sharing of the TEP1 protein between two seemingly unrelated RNPs suggests that TEP1 may play a more general role in RNP structure, function, or assembly. It is thus possible that TEP1 is a part of other RNPs in addition to the vault and telomerase complexes. Le and colleagues have also proposed that the telomerase RNA binding proteins hStau and L22 might be involved in aspects of RNA processing or RNP assembly that are not limited to telomerase (28). While the absence of a direct role for telomerase is confounding, the generation of mTep1-deficient mice is a first step toward the genetic dissection of the relationship between telomerase and the assembly and regulation of other cellular RNP complexes. We are currently examining mTep1-deficient mice for the role of TEP1 in vaults and for the possible involvement of TEP1 in other RNPs. In addition, the generation of mice deficient in other telomerase-associated components will enable us to ascertain whether TEP1 is genetically redundant with other telomerase RNA binding proteins for telomerase function in vivo.