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Molecular and Cellular Biology, June 2001, p. 3763-3774, Vol. 21, No. 11
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.11.3763-3774.2001

Vav1 Regulates Phospholipase Cgamma Activation and Calcium Responses in Mast Cells

Timothy Scott Manetz,1 Claudia Gonzalez-Espinosa,1 Ramachandran Arudchandran,1 Sandhya Xirasagar,1 Victor Tybulewicz,2 and Juan Rivera1,*

Section on Chemical Immunology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892-1820,1 and the National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom2

Received 3 November 2000/Returned for modification 12 December 2000/Accepted 7 March 2001


    ABSTRACT
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

The hematopoietic cell-specific protein Vav1 is a substrate of tyrosine kinases activated following engagement of many receptors, including Fcvarepsilon RI. Vav1-deficient mice contain normal numbers of mast cells but respond more weakly than their normal counterparts to a passive systemic anaphylaxis challenge. Vav1-deficient bone marrow-derived mast cells also exhibited reduced degranulation and cytokine production, although tyrosine phosphorylation of Fcvarepsilon RI, Syk, and LAT (linker for activation of T cells) was normal. In contrast, tyrosine phosphorylation of phospholipase Cgamma 1 (PLCgamma 1) and PLCgamma 2 and calcium mobilization were markedly inhibited. Reconstitution of deficient mast cells with Vav1 restored normal tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 and calcium responses. Thus, Vav1 is essential to Fcvarepsilon RI-mediated activation of PLCgamma and calcium mobilization in mast cells. In addition to its known role as an activator of Rac1 GTPases, these findings demonstrate a novel function for Vav1 as a regulator of PLCgamma -activated calcium signals.


    INTRODUCTION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

The early events following activation of the high-affinity receptor for immunoglobulin E (IgE) (Fcvarepsilon RI) on mast cells are well studied (32). Like other immunoreceptors, Fcvarepsilon RI contains multiple subunits, the IgE binding alpha  chain, and the beta  and gamma  chains that function to transduce signals via the paired tyrosine residues of the immunoreceptor tyrosine-based activation motifs (ITAMs) that are found within (45). Aggregation of multiple IgE-occupied Fcvarepsilon RI by polyvalent antigen (Ag) leads to transphosphorylation of the beta - and gamma -chain ITAMs by the associated Src family protein tyrosine kinase (PTK) Lyn (17, 41). This creates a new binding surface for Syk PTK, whose tandem Src homology 2 (SH2) domains facilitate the interaction with the ITAM, resulting in activation of this enzyme (4, 31). Activated Syk then phosphorylates multiple substrates, among which the linker for activation of T cells (LAT) is proximal and essential to Fcvarepsilon RI-activated responses (48). LAT, the SH2 domain-containing leukocyte phosphoprotein of 76 kDa (SLP-76), and phospholipase Cgamma (PLCgamma ) have all been implicated in the regulation of calcium responses in mast cells (40, 48, 59) and in other cells (12, 59, 64). These proteins, along with the Rac GTPase-activating Vav1 protein, interact to form a functional macromolecular complex at the plasma membrane that regulates both Rac and Ras signaling (1, 14, 21, 37, 51).

Vav1 is a cytosolic protein primarily expressed in hematopoietic cells whose structure and function have been extensively examined (6). Its structure includes a calponin homology (CH) domain, a Dbl homology (DH) domain, a pleckstrin homology (PH) domain, a single SH2 domain, and two Src homology 3 (SH3) domains that flank the SH2 domain. Functional studies demonstrated that tyrosine phosphorylation activates Vav's guanine nucleotide exchange factor (GEF) activity for Rho family GTPases (15) with a clear preference for Rac GTPases. Vav1 also promotes intracellular signaling by its C-terminal Grb2-like adapter region, which contains an SH2 domain flanked by two SH3 domains (47). Thus, because of its GEF and adapter functions Vav1 influences multiple cellular processes (47). Among these processes, the mobilization of calcium is intimately linked with Vav1 (5), as this is the most dominant defect in T-cell receptor (TCR)-stimulated Vav1-deficient T cells. TCR signaling in general also appears significantly reduced in Vav1-deficient thymocytes, with the resulting impairment in positive and negative selection allowing very limited numbers of T cells to mature and migrate into the periphery (18, 55). Though peripheral B-cell numbers were unaffected by Vav1 deficiency, the B-1a B-cell population in the peritoneal cavity was almost completely absent (63). In addition, the existing peripheral B cells displayed a reduced functional capacity following B-cell receptor stimulation. Unlike that of T cells, mast cell development is unimpaired by Vav1 deficiency (66); hence, exploring Vav1 function in mast cells avoids the possible secondary effects caused by abnormal cell development. Given the known similarities in intracellular signaling among the TCR, the B-cell Ag receptor, and Fcvarepsilon RI (29), investigation of Vav1 function in Fcvarepsilon RI-stimulated mast cells may likely reveal mechanisms common to all.


    MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Mice and BMMC culture. Vav1-/- mice and Vav1+/+ littermate controls on a mixed C57BL/6 and 129/Sv background were generated as described previously (57). Animals were maintained and used in accordance with National Institutes of Health (NIH) guidelines. Bone marrow mast cell (BMMC) cultures were established from 4- to 8-week-old mice as previously described (48). Mast cell differentiation was confirmed by toluidine blue staining and measurement of IgE receptor expression as determined by flow cytometry. Cultures were utilized only when >= 90% of the cells stained positive. IgE receptor expression was determined by incubating approximately 106 cells at 37°C with 1.5 µg of anti-2,4-dinitrophenol (DNP) IgE antibody (Ab) for 3 h. Following washing, the cells were incubated at 4°C for 5 min with a 0.5-µg concentration of Fc Block (PharMingen) followed by a 0.5-µg concentration of fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgE Ab in a standard staining buffer (45 min). After washing, the cells were analyzed for surface IgE on a flow cytometer and compared to an isotype control.

Reagents and Abs. Anti-DNP IgE Ab was produced as previously described (34). DNP-human serum albumin (DNP-HSA; Ag; Sigma) was diluted in phosphate-buffered saline (PBS) prior to use. For virus-mediated gene transfer experiments, the pSFV1 expression system, Vav1 constructs, and infection procedure were as previously described (2, 51). Briefly, rat cDNAs of Vav1 and of a Vav1 with the Dbl domain (K194 to I405) deleted were subcloned into the AvrII and NsiI sites of a modified pSFV1 plasmid that contained a multiple cloning site and an in-frame green fluorescent protein (GFP) cassette. For virus production, mRNA was generated in vitro from the pSFV1-Vav1 constructs and from the helper pSFV2 (which encodes viral coat proteins) and electroporated into baby hamster kidney cells to generate virus (2). BMMCs (107) were infected, with all procedures performed 5 h following initial infection. The Vav1 with the Dbl domain deleted was previously shown to be unable to activate the Rac1-JNK pathway in mast cells (56) but able to translocate to the plasma membrane upon aggregation of Fcvarepsilon RI, like wild-type Vav1 (51). Abs used for immunoprecipitation were as follows: mouse anti-Fcvarepsilon RI beta  chain (46), chicken anti-Fcvarepsilon RI gamma  chain (36), rabbit anti-Syk (gift of U. Blank, Institut Pasteur), rabbit anti-LAT, rabbit anti-Vav, mixed mouse monoclonal anti-PLCgamma 1, rabbit anti-PLCgamma 2, goat anti-SLP-76, goat anti-ERK2, and goat anti-JNK1 (Santa Cruz Biotechnology). Abs used for immunoblotting were as follows: mouse anti-Fcvarepsilon RI beta  chain, chicken anti-Fcvarepsilon RI gamma  chain, mouse anti-Syk (gift of P. Draber, Institute of Molecular Genetics, Prague, Czech Republic), rabbit anti-LAT serum (gift of L. E. Samelson, National Cancer Institute, NIH), mouse anti-Vav, mixed mouse monoclonal anti-PLCgamma 1, rabbit anti-PLCgamma 2, rabbit anti-SLP-76, rabbit anti-ERK2, rabbit anti-JNK1 (Santa Cruz), mouse anti-phospho-ERK2, rabbit anti-phospho-JNK (NEN), rabbit anti-Akt, rabbit anti-phospho-Akt (P-Ser 472/473/474; PharMingen), and mouse antiphosphotyrosine (4G10-biotin; Upstate Biotechnology). Secondary Abs used for blotting were as follows: sheep anti-mouse IgG-horseradish peroxidase (HRP), donkey anti-rabbit IgG-HRP (Amersham), mouse anti-rabbit Ig-biotin, rabbit anti-goat IgG-HRP (Sigma), and rabbit anti-chicken IgY-HRP (Jackson Immunoresearch). Extravidin peroxidase conjugate (Sigma) was used as a secondary reagent for all biotin-conjugated Abs.

Lysate preparation, immunoprecipitation, and immunoblotting. For the lysate preparation, cells (106/ml) were incubated in interleukin-3 (IL-3)-deficient medium containing 0.5 µg of anti-DNP IgE Ab/ml (preincubation) for 4 h. After preincubation, washing, and resuspension in Tyrodes-0.05% bovine serum albumin (BSA) (Tyrodes consists of 10 mM HEPES, 130 mM NaCl, 5 mM KCl, 1.4 mM CaCl2, 1 mM MgCl2, and 5.6 mM glucose), the cells (30 × 106/ml) were stimulated for various times with 100 ng of DNP-HSA/ml. Reactions were terminated by the addition of ice-cold PBS containing pyrophosphate, orthovanadate, and 50 mM EGTA. After washing in the same PBS solution, cells were lysed on ice using 1% NP-40 containing Tris-HCl (pH 7.5), 60 mM beta -octylglucoside, pyrophosphate, orthovanadate, aprotinin, leupeptin, and phenylmethylsulfonyl fluoride for 30 min as described previously (52). Immunoprecipitations and immunoblotting were previously described (1). Relative quantitation of immunoblots was performed by densitometry.

Systemic anaphylaxis and histology. The anaphylaxis method used was described previously (40). Briefly, animals were sensitized with 3 µg of DNP-specific IgE Ab by intravenous tail vein injection. The animals were subsequently challenged (24 h) by intravenous tail vein injection with vehicle (PBS) or 500 µg of DNP-HSA. After 1.5 min, mice were sacrificed, blood samples were immediately obtained by cardiac puncture, and the serum was isolated. Serum histamine levels were determined according to the manufacturer's protocol using a histamine inhibition enzyme-linked immunosorbent assay kit (Immunotech). Determination of skin mast cell number was previously described (48).

Phosphatidylinositol-3,4,5-trisphosphate (PIP3), inositol-1,4,5-trisphosphate (IP3), and calcium measurements. PIP3 measurements were performed by a modification of the previously described procedure (42). Briefly, BMMCs were incubated in the absence of IL-3 but in the presence of 1 µg of IgE/ml for 4 h at 37°C. Cells were washed twice with Tyrodes-BSA buffer and resuspended in the same buffer at 5 × 106 cells/ml in the presence of 120 µCi of [32P]orthophosphate/ml. After a 90-min incubation at 37°C, the cells were washed once with Tyrodes-BSA and resuspended at 107 cells/ml. Wortmannin (100 nM) was added to the corresponding tubes 15 min before Ag stimulation. Stimulation was performed with 200 ng of Ag/ml at 37°C for 1 or 5 min. The reaction was stopped by microcentrifuging the cells for 10 s at 4°C and resuspending the pellet in 750 µl of methanol-1.2 N HCl (1:1 [vol/vol]). Twenty micrograms of PIP3 and phosphoinositides from bovine brain was added as a carrier before the addition of 390 µl of chloroform. Tubes were vortexed, and the organic phase was collected after centrifugation and reextracted with 400 µl of methanol-0.1 M EDTA (pH 8.0). The new organic (lower) phase was recovered after centrifugation, and 200 µl was dried using a Savant evaporator. Phospholipids were resuspended in 25 µl of chloroform and applied to thin-layer chromatography (TLC) plates precoated with 1.2 M potassium oxalate. Phosphoinositides (Sigma) of known composition were used as a standard. The separation was performed in a mixture of chloroform-acetone-methanol-acetic acid-water (80/30/26/24/14 [vol/vol/vol/vol/vol]). Chromatography was performed for 4 h, and labeled phospholipids were detected using Molecular STORM. The migration of each phospholipid was confirmed by exposing the plates to iodine vapors.

IP3 production was measured as described by Choi et al. (10), with a minor modification. Briefly, 107 BMMCs (suspension cells) were stimulated in PIPES [piperazine-N,N'-bis(2-ethanesulfonic acid)] buffer (500 µl) in the absence or presence of 100 ng of Ag, and the reaction was terminated by adding 100 µl of ice-cold 100% trichloroacetic acid. IP3 was then extracted from the samples and quantified using a commercially available assay kit (Dupont/NEN).

Calcium was measured as described previously (48). Briefly, cells (2 × 106) were dually loaded with either 16 µM Fluo-3-AM or 16 µM Fura Red (Molecular Probes) in RPMI-2% fetal calf serum for 45 min at 37°C. The cells were then incubated with IgE (1 µg/106 cells) on ice for 1 h and brought to room temperature for 20 min. The cells were resuspended in Tyrodes-BSA, and changes in dye fluorescence with time were determined by flow cytometry, after stimulation with 30 ng of Ag/ml followed by 1 µM thapsigargin at 37°C. Calcium mobilization is reported as the Fluo-3/Fura Red fluorescence intensity ratio over time. For the virus-mediated gene transfer experiments with GFP or Vav-GFP, 107 cells per sample were stained with Fura Red, and the GFP-expressing (GFP+) cells (based on a histogram gate compared to noninfected cells) were analyzed for Fura Red fluorescence over time. The percentage of GFP+ cells varied from 2.5 to 25%.

Degranulation. Degranulation was determined by beta -hexosaminidase release (44). Cells (106) were stimulated with indicated concentrations of DNP-HSA for up to 30 min at 37°C in 0.5 ml of Tyrodes-0.05% BSA and then placed on ice. Cells were centrifuged (280 × g at 4°C for 10 min), and supernatants were collected. Cell pellets were lysed (30 min) in 0.5% Triton X-100 (0.5 ml), and the supernatant and pellet (30-µl) samples were incubated for 1 h at 37°C with 1 mM p-nitrophenyl-N-acetyl-beta -D-glucosamide (30 µl) in 0.1 M sodium citrate buffer on a 96-well plate. The incubation was terminated by the addition of 0.1 M Na2CO3-NaHCO3 buffer (200 µl), and the optical density was read on a plate reader at a 405-nm wavelength. Net degranulation is expressed as the percentage of total cellular beta -hexosaminidase released to the medium following Ag stimulation minus that released prior to Ag stimulation (spontaneous release). Spontaneous release did not differ significantly among the phenotypes for all of the experiments.

RT-PCR. After preincubation, washing, and resuspension in Tyrodes-BSA, the cells (30 × 106 total in 5 ml) were stimulated for various times with 1 ng of DNP-HSA/ml. The reaction was terminated by the addition of TRI reagent (Molecular Research Center, Inc.). The RNA was extracted using chloroform and precipitated with isopropanol. First-strand cDNA was synthesized using 5 µg of total RNA with the Life Technologies Superscript reverse transcription-PCR (RT-PCR) kit. Using 7.5 µl of the first-strand cDNA reaction, amplification of the individual cytokines was performed using specific primers (Clontech) under the following conditions: 96°C for 1 min; 2 cycles of 96°C for 1 min and 60°C for 4 min; 28 cycles (IL-4, IL-6, tumor necrosis factor alpha, and monocyte chemoattractant protein 1) or 35 cycles (IL-2, IL-3, IL-10, and gamma interferon [IFN-gamma ]) of 94°C for 1 min, 60°C for 2.5 min, and 72°C for 4 min; 72°C for 10 min; and 4°C for 6 h. Most cytokines amplified under these conditions show a linear increase in detectable levels with corresponding increases in cDNA. This allowed relative quantitation of cytokine levels when normalized to the mRNA levels of a housekeeping gene like glyceraldehyde-3-phosphate dehydrogenase or when a competitor cDNA was used, as we previously described (8, 54). The products were resolved by 2% agarose gel electrophoresis.


    RESULTS
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

To explore the importance of Vav1 in mast cell responses, we tested Vav1-deficient (Vav1-/-) animals for their ability to undergo passive systemic anaphylaxis. Ag challenge of animals previously sensitized with DNP-specific IgE Ab showed that Vav1-/- mice displayed a reduced ability to undergo systemic anaphylaxis compared with that of Vav1+/+ mice (Fig. 1A). This decrease was not due to Vav1-deficient mice possessing fewer mast cells, as the number of skin mast cells and their granule phenotype, as determined by histochemical staining, were unchanged among Vav1+/+, Vav1+/-, and Vav1-/- mice (Fig. 1B and data not shown). This suggested that the decreased serum histamine levels in Vav1-/- mice likely resulted from an Fcvarepsilon RI-mediated functional defect and not a defect in mast cell development.


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  		FIG. 1.   Effect of Vav1 deficiency on passive systemic anaphylaxis, IgE receptor expression, and Vav tyrosine phosphorylation. (A) Vav+/+ (squares), Vav+/- (triangles), and Vav-/- (inverted triangles) mice (n = 12 per dose group) were passively sensitized and challenged to induce systemic anaphylaxis as described in Materials and Methods. The serum histamine concentration (nanomolar concentration) was determined via inhibition enzyme-linked immunosorbent assay and compared among PBS (filled symbols)- and Ag (open symbols)-challenged animals, with the mean for each dose group indicated by a line. The asterisk indicates a significance of P <=  0.03 (t test) for Vav-/- responders compared to Vav+/+ mice. No significant difference was found between Vav+/+ and Vav+/- mice. (B) A section of depilated dorsal skin was removed from each animal (n = 2 per phenotype), and three sections per skin sample were stained with toluidine blue for mast cells. Mast cell number was assessed at a magnification of ×200 in random fields. (C) Vav+/+ (blue), Vav+/- (red), and Vav-/- (black) BMMC cultures (4 weeks) were sensitized with IgE and stained with an FITC-conjugated anti-IgE Ab to indirectly determine BMMC surface IgE receptor expression by flow cytometry. Nonspecific binding was determined by an FITC-conjugated isotype control (yellow). (D) Vav was immunoprecipitated from lysates of BMMCs (30 × 106) stimulated or not with 100 ng of Ag for 2 min, resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to a nitrocellulose membrane. Tyrosine phosphorylation was determined by blotting with the antiphosphotyrosine Ab (PY, upper panel). After stripping, protein levels were determined by reblotting with anti-Vav Ab (lower panel). IP, immunoprecipitation; IB, immunoblotting.

To investigate the underlying mechanism responsible for this physiological defect, all subsequent experiments were performed on BMMCs cultured from the bone marrow of Vav1-/-, Vav1+/-, and Vav1+/+ mice, all of which developed normally. By 4 to 5 weeks of culture, the fraction of cells expressing Fcvarepsilon RI (Fig. 1C) was more than 95% and the staining of mast cell granule content by toluidine blue or alcian blue-safranin (data not shown) was comparable among Vav1-/-, Vav1+/-, and Vav1+/+ cells. A gene dose-dependent decrease in Fcvarepsilon RI-induced tyrosine phosphorylation of Vav1 corresponded with the loss of Vav1 protein (Fig. 1D). To correlate the previous systemic anaphylaxis results with mast cell degranulation in vitro, BMMC degranulation was compared among the Vav1+/+, Vav1+/-, and Vav1-/- phenotypes (Fig. 2A). Fcvarepsilon RI-mediated degranulation (indicated by beta -hexosaminidase release) was significantly reduced in Vav1-/- cells compared to Vav1+/+ cells, although the total amount of beta -hexosaminidase per cell did not differ among phenotypes. The most dramatic reduction (63%) was at suboptimal concentrations of Ag (3 ng/ml), where the response was linear, but even at optimal Ag concentrations a reduction of more than 30% was detected. This decrease was due, in part, to slower kinetics of release by Vav1-/- cells (Fig. 2B). Interestingly, stimulation with thapsigargin (Fig. 2A, inset) or ionomycin (data not shown) did not reconstitute the degranulation response in Vav-/- cells.


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  		FIG. 2.   Vav1 deficiency affects mast cell degranulation and cytokine mRNA levels. (A) Degranulation (percentage of total beta -hexosaminidase release) was measured following 30 min of stimulation with various doses of Ag among IgE-sensitized Vav+/+ (squares), Vav+/- (triangles), and Vav-/- (inverted triangles) BMMCs. Values represent an average of six independent dose response experiments per phenotype. Degranulation was also measured following 30 min of stimulation with 250 ng of thapsigargin (inset). (B) Cells of all three phenotypes (as described above) were stimulated with 9 ng of Ag for 0 to 30 min, and the percentage of total beta -hexosaminidase release was determined. Values represent an average of four independent kinetic experiments for Vav+/+ and Vav-/- cells and three experiments for Vav+/- cells. For panels A and B, the single asterisks represent P <=  0.05 and the double asterisks represent P <=  0.01 for Vav+/+ cells compared to Vav-/- cells at that Ag concentration (A) or time point (B), as determined by t test. (C) The kinetics of BMMC cytokine mRNA levels among Vav+/+, Vav+/-, and Vav-/- cells were determined from unstimulated cells (0 h) or cells stimulated for up to 2 h with 1 ng of Ag. Cytokine mRNA levels were determined by RT-PCR as described in Materials and Methods. One representative of five experiments is shown. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MCP-1, monocyte chemoattractant protein 1; TNFalpha , tumor necrosis factor alpha.

Mast cell cytokine production is another important component of the mast cells' physiological role in health and disease (see reference 20 for a review). Since activated Vav1-/- splenic T cells have a reduced capacity to produce IL-2 (55), there was an interest in determining whether Vav1 regulates expression of similar genes in mast cells. The mRNA profile of a variety of cytokines was examined by RT-PCR following Fcvarepsilon RI stimulation, as we previously demonstrated that cytokine mRNA levels correlate with cytokine production and secretion (48). While Vav1 deficiency did not appreciably alter the kinetics of mRNA expression, the changes observed in mRNA levels were gene dose dependent. IL-2 and IFN-gamma were the most significantly affected; relative quantitation (8, 54) revealed an 85.4% ± 6.9% inhibition of IL-2 mRNA and a 60.4% ± 15.0% inhibition of IFN-gamma mRNA in Vav1-/- cells compared to that in Vav1+/+ cells (Fig. 2C). IL-3, IL-4, IL-6, and tumor necrosis factor alpha mRNA levels were also decreased, though not to the extent of IL-2 and IFN-gamma . In most experiments, IL-10 mRNA was increased while the chemokine monocyte chemoattractant protein 1 was only minimally affected in Vav1-/- cells (Fig. 2C). That Vav1 deficiency inhibited IL-2 and IFN-gamma mRNA levels more dramatically than those of other cytokines suggested that Vav's influence on Fcvarepsilon RI-mediated cytokine mRNA expression is rather selective.

Vav's role in Fcvarepsilon RI signaling was explored by analyzing the tyrosine phosphorylation of proteins from Vav1+/+, Vav1+/-, and Vav1-/- BMMCs following Fcvarepsilon RI stimulation. For all proteins investigated, no change in the overall expression level was observed among the three phenotypes. Following Fcvarepsilon RI stimulation, tyrosine phosphorylation of Fcvarepsilon RI beta  and gamma  chains or of Syk was unaffected by Vav1 deficiency at 2 min (the peak time of receptor phosphorylation [Fig. 3]). However, in Vav1-/- cells, phosphorylation of Fcvarepsilon RI beta  and gamma  chains was partly decreased (33.1% ± 7.7%) at 10 min poststimulation compared to that in Vav1+/+ cells, suggesting a more transient phosphorylation of Fcvarepsilon RI in the absence of Vav1. No significant difference in Fcvarepsilon RI phosphorylation was observed for Vav+/- cells, and Syk phosphorylation appeared to be identical for all three genotypes at the indicated time. The profile of Fcvarepsilon RI-induced tyrosine-phosphorylated proteins for each phenotype demonstrated that the loss of Vav1 led to the increased tyrosine phosphorylation of a 42-kDa protein (Fig. 4A). Because this approximates the molecular mass of the mitogen-activated protein kinases ERK2 and JNK1, which are known to regulate gene expression (53), we investigated their Fcvarepsilon RI-dependent activation. ERK2 phosphorylation was significantly increased (approximately twofold) in Vav1+/- and Vav1-/- cells compared to that in Vav1+/+ cells, suggesting that it was the 42-kDa protein that was detected in the previous antiphosphotyrosine experiment (Fig. 4B). In contrast, JNK1 phosphorylation was decreased by up to 53% (inhibition ranged from 29 to 53% at the peak phosphorylation time of 15 min) in the absence of Vav1 (Fig. 4C).


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  		FIG. 3.   Tyrosine phosphorylation of the IgE receptor and Syk is Vav1 independent. Lysates were prepared from IgE-sensitized Vav+/+, Vav+/-, and Vav-/- BMMCs (30 × 106/ml) stimulated for 0, 2, or 10 min with 100 ng of Ag. Fcvarepsilon RI beta  chain (A), Fcvarepsilon RI gamma  chain (B), and Syk (C) were immunoprecipitated, resolved on sodium dodecyl sulfate-polyacrylamide gels, and transferred to nitrocellulose membranes. Tyrosine phosphorylation (PY) was determined by blotting with the antiphosphotyrosine Ab (upper panels). Individual protein levels were determined by reblotting with protein-specific Abs (lower panels). Fcvarepsilon RI beta  protein is identified with an arrow to distinguish it from Ig light chain (IgLC). One representative of three experiments is shown. IP, immunoprecipitation; IB, immunoblotting.


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  		FIG. 4.   Tyrosine phosphorylation of cell lysate proteins, ERK2, and JNK1 is differentially affected by Vav1 deficiency. Lysates were prepared from IgE-sensitized Vav+/+, Vav+/-, and Vav-/- BMMCs (30 × 106/ml) stimulated for 0, 2, or 10 min with 100 ng of Ag. Total cell lysates (A) for the three phenotypes were resolved on a sodium dodecyl sulfate-polyacrylamide gel and transferred to a nitrocellulose membrane. ERK2 (B) and JNK1 (C) were immunoprecipitated and resolved as described above. Tyrosine phosphorylation (PY) was determined by blotting with the antiphosphotyrosine Ab (upper panel, A) or with phosphospecific Abs to ERK2 and JNK (upper panels, B and C, respectively). Individual protein levels were determined by reblotting with protein-specific Abs (lower panels) for ERK2 and JNK1, while the cell lysates were probed for protein content with anti-Lyn Ab. One representative of a minimum of three experiments is shown. IB, immunoblotting; IP, immunoprecipitation.

The inhibition of degranulation, cytokine production, and JNK1 activation by Vav1 deficiency suggested that Vav1 is likely to regulate an event common to all these responses. Therefore, because we previously demonstrated the presence of a Vav1-containing Fcvarepsilon RI-induced LAT-organized signaling complex that is critical to mast cell responses (1, 48), we hypothesized that the defect should result from impaired complex formation and/or activation. LAT phosphorylation was unchanged in Vav1-/- cells (Fig. 5A). Because Vav and SLP-76 were previously shown to interact and synergize in the production of IL-2 in T cells (62), we examined the activation of SLP-76 as measured by its phosphorylation. SLP-76 phosphorylation was significantly enhanced in nonactivated and activated Vav1+/- and Vav1-/- cells compared to their wild-type counterpart (Fig. 5B). Because Syk kinase phosphorylates both Vav1 and SLP-76, the absence of Vav1 could allow more SLP-76 to interact with Syk, thus causing enhanced phosphorylation. Nevertheless, it is clear that the phosphorylation of LAT and SLP-76 occurs in the absence of Vav1. The tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 was also investigated, since PLCgamma was previously demonstrated to associate with LAT (65). Tyrosine phosphorylation of PLCgamma 1 decreased in a gene dose-dependent manner with the loss of Vav1. Little or no detectable phosphorylated protein was evident 10 min following stimulation of Vav1-/- cells (Fig. 5C). Tyrosine phosphorylation of PLCgamma 2 was also affected, although differences were less apparent between Vav1+/+ and Vav1+/- cells, whereas a dramatic decrease was observed in Vav1-/- cells (Fig. 5D). This demonstrated that the absence of Vav1 protein caused a dramatic inhibition in the Fcvarepsilon RI-induced tyrosine phosphorylation of both PLCgamma 1 and PLCgamma 2. Thus, as might be expected, PLCgamma activity was significantly reduced, with peak IP3 levels inhibited by approximately 50% in the absence of Vav1 (Fig. 6A). To determine if the loss of PLCgamma 1 and PLCgamma 2 phosphorylation and activity resulted from a decreased association with LAT, we immunoprecipitated LAT from mast cells of all three phenotypes and immunoblotted for PLCgamma 1 and PLCgamma 2. Figure 6B demonstrates that PLCgamma 1, PLCgamma 2, and SLP-76 were recruited to LAT even in the absence of Vav1. The association was dependent on Fcvarepsilon RI stimulation with no significant differences in association of PLCgamma 1 and PLCgamma 2 with LAT observed at the peak of phosphorylation (2 min). At 10 min poststimulation, all three experiments showed slightly less (10.2 to 33.5%) of PLCgamma 1 and PLCgamma 2 coimmunoprecipitating with LAT derived from Vav1-/- mast cells, suggesting a slightly more transient association of PLCgamma 1 and PLCgamma 2 with LAT in the absence of Vav1. Thus, Vav1 is not required for PLCgamma 1 and PLCgamma 2 interaction with LAT but is required for PLCgamma tyrosine phosphorylation.


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  		FIG. 5.   Tyrosine phosphorylation of LAT and SLP-76 is intact in Vav1-deficient mast cells, whereas tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 is inhibited. Lysates were prepared from IgE-sensitized Vav+/+, Vav+/-, and Vav-/- BMMCs (30 × 106/ml) stimulated for 0, 2, or 10 min with 100 ng of Ag. LAT (A), SLP-76 (B), PLCgamma 1 (C), and PLCgamma 2 (D) were immunoprecipitated, resolved on sodium dodecyl sulfate-polyacrylamide gels, and transferred to nitrocellulose membranes. Tyrosine phosphorylation (PY) was determined by blotting with the antiphosphotyrosine Ab (upper panels). Individual protein levels were determined by reblotting with protein-specific Abs (lower panels). A representative of a minimum of three experiments is shown. IP, immunoprecipitation.



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  		FIG. 6.   Vav1 deficiency inhibits IP3 and PIP3 production but does not affect the association of PLCgamma 1, PLCgamma 2, and SLP-76 with LAT. (A) IP3 levels from Vav1+/+ (squares) and Vav-/- (triangles) BMMCs were measured prior to and after stimulation with 100 ng of Ag. Samples were collected at the indicated times. (B) Lysates were prepared from IgE-sensitized Vav+/+, Vav+/-, and Vav-/- BMMCs (30 × 106/ml) stimulated for 0, 2, or 10 min with 100 ng of Ag. LAT was immunoprecipitated, resolved on a sodium dodecyl sulfate-polyacrylamide gel, and transferred to a nitrocellulose membrane. PLCgamma and SLP-76 coimmunoprecipitating with LAT were determined by immunoblotting with Abs to PLCgamma 1, PLCgamma 2, and SLP-76. The amount of LAT was subsequently determined by reblotting with anti-LAT Abs (lower panel). One representative of three experiments is shown. IP, immunoprecipitation; IB, immunoblotting. (C) PIP3 levels in [32P]orthophosphate-labeled Vav+/+ or Vav-/- mast cells were measured prior to and following Ag stimulation (200 ng/ml) for the indicated time in the absence (-) or presence (+) of wortmannin. One representative experiment for TLC is shown. The TLC separation pattern shown was determined from a phosphoinositide standard of known composition. A prolonged exposure is shown to detect PIP3 signals. Results of the quantitation of all experiments are shown in the graph. PIP3 levels were normalized to nonstimulation conditions (where 0 min = 100%). PIP3 levels in the presence of wortmannin were arbitrarily set to the levels of nonstimulated cells (100%), as no signal was detectable. WT, wild type; KO, knockout; PS, phosphatidylserine; PI, phosphatidylinositol. (D) Akt activation is more transient in Vav-/- than in Vav+/+ mast cells. Cells were activated as described above, and Akt phosphorylation was measured from whole-cell lysates with an anti-Akt phosphospecific Ab. One representative of three experiments is shown. WT, wild type; KO, knockout.

Because phosphatidylinositol 3-kinase (PI3K) activity is required for membrane recruitment and activation of PLCgamma as well as for calcium responses (3, 43), we determined the PI3K activity in Vav+/+ and Vav-/- mast cells by measuring the amount of PIP3 generated in response to Fcvarepsilon RI. That Vav1 could have an effect on PI3K activity is supported by various studies, including the previously described interaction between these proteins (50). Measurement of PIP3 levels in Ag-stimulated cells, in the presence or absence of the PI3K inhibitor wortmannin, revealed a more transient production of PIP3 in the Vav1-/- mast cells than in Vav+/+ cells (Fig. 6C). At 5 min poststimulation, almost complete loss of PIP3, compared to that in Vav+/+ cells, was observed in Vav-/- cells (Fig. 6C). Because PI3K activity is required for the activation of protein kinase B/Akt (19), we measured the activation of Akt in Vav+/+ and Vav-/- cells by use of a phosphospecific Ab. Figure 6D shows that Akt activation in Vav-/- mast cells is more transient than that observed in Vav+/+ cells, thus confirming a role for Vav1 in the sustained activation of PI3K and Akt.

Activation of PLCgamma was demonstrated to be critical for its function in generating the second messenger IP3 (39), which regulates calcium mobilization; thus, we next investigated calcium responses in mast cells. This line of investigation was supported by previous reports of reduced calcium mobilization in Vav1-/- T cells and B cells (18, 57). Calcium mobilization was monitored following stimulation with Ag, and a gene dose-dependent decrease in calcium mobilization was found, with the lowest calcium response being observed for Vav1-/- cells (Fig. 7A). Interestingly, subsequent stimulation with thapsigargin also showed lowered calcium responses from Vav1-/- cells. Consistent with a decrease in IP3 production, the decreased mobilization of calcium also correlated with the reduced degranulation following Ag or thapsigargin stimulation of Vav1-/- BMMCs (Fig. 2A). Depletion of extracellular calcium and addition of EGTA to the medium did not cause a change in the observed differences of the calcium profiles of all three phenotypes, although the extent of the response for each phenotype was reduced (data not shown). This suggested that the mobilization of calcium from intracellular stores was reduced in the absence of Vav1, consistent with the observed defect in PLCgamma activation.


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  		FIG. 7.   Calcium mobilization and tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 are Vav1 dependent. (A) IgE-sensitized Vav1+/+ (blue), Vav+/- (red), and Vav-/- (yellow) cells (2 × 106) in Tyrodes-0.05% BSA were loaded with Fluo-3-AM and Fura Red AM for determination of calcium mobilization by flow cytometry. Cells were stimulated with 30 ng of Ag followed by 1 µM thapsigargin (as indicated). Data shown are ratios of Fluo-3 and Fura Red dye fluorescence measured over time. One representative of four experiments is shown. (B and C) For reconstitution of PLCgamma phosphorylation (B) and the calcium response (C), cells were infected with a GFP-expressing viral vector or one containing Vav1-GFP and loaded with Fura Red AM only (C). (B) Lysates were prepared from Vav1+/+ BMMCs expressing GFP (GFP; lanes 1 and 2), Vav1-/- BMMCs expressing GFP (GFP; lanes 3 and 4), Vav1-/- BMMCs expressing Vav1-GFP (lanes 5 and 6), or Vav1-/- BMMCs expressing Vav1-GFP with the Dbl domain deleted (-DH) (lanes 7 and 8) following 0 or 2 min of stimulation with 100 ng of Ag. PLCgamma 1 and PLCgamma 2 were immunoprecipitated, resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and transferred to nitrocellulose membranes. Tyrosine phosphorylation (PY) was determined by blotting with the antiphosphotyrosine Ab (upper panels). Individual protein levels were determined by reblotting with protein-specific Abs (lower panels). One representative experiment is shown. The fold induction was determined by densitometric analysis of a minimum of three experiments, and the intensity of the phosphotyrosine signal was normalized to the amount of immunoprecipitated protein. Standard deviations ranged from 7 to 22% of the values shown. IP, immunoprecipitation; IB, immunoblotting. (C) Infected BMMCs were identified by GFP fluorescence. The calcium response of Vav1+/+ cells expressing GFP (blue), Vav1-/- cells expressing GFP (pink), Vav1-/- cells expressing -DH Vav1-GFP (teal), or Vav1-/- cells expressing Vav1-GFP (yellow) was assessed following stimulation as described above. Data are presented as Fura Red intensities for GFP+ cells over time.

We tested the postulate that reconstitution of Vav1 in Vav1-/- cells would lead to reconstitution of the tyrosine phosphorylation and activation of PLCgamma 1 and PLCgamma 2. We reasoned that Vav could contribute to the tyrosine phosphorylation of PLCgamma by facilitating the interaction of a tyrosine kinase with PLCgamma in this complex, as Syk and other tyrosine kinases (16, 35) interact with Vav1. Since activation of PLCgamma 1 and PLCgamma 2 is required for normal mast cell calcium responses (40, 48, 59), we also measured calcium responses following reconstitution with Vav1. We introduced Vav1 with an enhanced GFP tag (Vav1-GFP) or the GFP vector alone (as a control) into Vav+/+ or Vav1-/- cells using a previously described Semliki Forest virus vector (2). Ag stimulation of GFP-expressing Vav+/+ cells showed an increase of 13.3- and 15.6-fold in the tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2, respectively. Expression of GFP alone in Vav1-/- cells followed by Ag stimulation showed some increased tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 (2.4- and 3.7-fold increase, respectively) compared to that for nonstimulated conditions. However, when PLCgamma 1 and PLCgamma 2 were immunoprecipitated from Vav-GFP-reconstituted Vav1-/- cells, a dramatic increase in the Fcvarepsilon RI-stimulated tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 (10.7- and 11.4-fold increase, respectively) was detected (Fig. 7B). This demonstrates that Vav1 is critical to the tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2. To further address the underlying mechanism, we asked if the GEF activity of Vav1 is required for tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2. Vav-/- mast cells were reconstituted with a Vav-GFP construct in which only the DH domain (K194 to I405) was deleted (-DH). Immunoprecipitation of PLCgamma 1 and PLCgamma 2 from nonstimulated and stimulated cells, in three individual experiments, revealed that tyrosine phosphorylation of PLCgamma 1 is relatively independent of the GEF activity of Vav1 (average 10.0-fold increase in three experiments) whereas that of PLCgamma 2 (average 2.1-fold increase in three experiments) is dependent (Fig. 7B). Thus, the results show that Vav1 regulates the tyrosine phosphorylation of PLCgamma 1 and PLCgamma 2 differentially.

Because GFP fluoresces at the same wavelength as does Fluo-3, for calcium studies, we preloaded cells with Fura Red alone as the indicator for increased free calcium. Thus, the results are expressed as an increase in intensity of Fura Red fluorescence rather than the fluorescence ratio of two dyes (Fig. 7A versus C). Following reconstitution with Vav1-GFP, the ability of the infected (GFP+) cells to mobilize calcium upon Fcvarepsilon RI activation was examined. As expected, and comparable to dual dye experiments, Vav1-/- (GFP+) cells showed a significantly diminished calcium response compared to that of Vav1+/+ cells (Fig. 7C). Additionally, a test of whether the GEF activity of Vav1 is required for the reconstitution of the calcium response revealed that the absence of this activity results in a defective calcium response identical to that in Vav1-/- (GFP+) cells. However, when the Vav1-/- BMMCs were reconstituted with Vav1-GFP, the calcium mobilization following Ag stimulation was comparable to that in GFP-infected wild-type mast cells (Fig. 7C). Unlike the Vav1-/- (GFP+) or Vav (DH-)-GFP+ reconstituted cells, reconstitution of Vav1-GFP in Vav-/- mast cells reconstituted the thapsigargin-induced calcium response. However, although all experiments showed reconstitution of the calcium response activated by thapsigargin, the level of the reconstituted response was more variable than that seen with Ag stimulation. Preliminary experiments also showed that, in the absence of extracellular calcium, and in the presence of EGTA, Vav1 reconstitution of the calcium response still occurred (data not shown) consistent with reconstitution of release from intracellular stores. Collectively, these data demonstrate that decreased PLCgamma activation and calcium mobilization are reversed by the introduction of competent Vav1.


    DISCUSSION
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

The present study shows that Vav1 deficiency in mast cells results in a well-defined phenotype. We found no obvious role for Vav1 in the development of mast cells. Activation of Fcvarepsilon RI-proximal PTKs was also intact, as demonstrated by normal levels of beta  and gamma  chains and Syk tyrosine phosphorylation. Most signals downstream of Syk PTK activation were also intact, in the absence of Vav1, as the tyrosine phosphorylation of LAT was unaffected and tyrosine phosphorylation of SLP-76 and ERK2 was enhanced. Signaling deficiencies were observed for molecules that regulate calcium responses or are regulated by intracellular calcium; thus, tyrosine phosphorylation of PLCgamma 1, PLCgamma 2, and JNK1 was defective.

Vav1 was reported to activate JNK and gene expression in mast cells (52, 56). Because JNK activity was implicated in the regulation of IL-2 mRNA stability and expression (9), our observation of a dramatic loss of IL-2 mRNA is consistent with the observed partial loss of JNK1 activity. The loss of calcium responses is also a likely explanation for reduced IL-2 responses, as calcium is required for synergy of calcineurin and PKCtheta in activation of JNK1 and IL-2 production (60). That Vav1 deficiency mainly affected IL-2 and IFN-gamma was also not surprising. IL-2 and IFN-gamma expression are regulated by NF-AT, whose activation is exquisitely sensitive to calcium (7, 58), and Vav1 regulates the activation of NF-AT in T cells (61). In contrast, an increased IL-10 mRNA level was observed in Vav1-deficient cells. This correlated with increased phosphorylation of ERK2 and SLP-76; however, a causal relationship remains to be established.

Genetic studies of mast cell function have revealed overlapping phenotypes in the absence of LAT (48), SLP-76 (40), PLCgamma 2 (59), and now Vav1 expression. As all of these proteins are linked by participation in a macromolecular signaling complex (1), it is not surprising that the absence of one may impinge on the function of others. A theme common to all is the regulation of calcium responses. Little is known of the phosphorylation status of the other signaling proteins in the absence of PLCgamma 1 (27) or PLCgamma 2 (59), although, for the former, activation of ERK2 was found to be prolonged in murine embryonic fibroblasts (26), consistent with our observation of enhanced ERK2 activation in the absence of Vav1. However, it should be noted that an opposite effect has been reported previously for PLCgamma 2-deficient B cells (22), thus suggesting cell-specific differences in the regulation of ERK2. In Vav1-deficient mast cells, phosphorylation of LAT and SLP-76 was not adversely affected, whereas phosphorylation of PLCgamma 1 and PLCgamma 2 was inhibited. In SLP-76-deficient mast cells, tyrosine phosphorylation of PLCgamma 1 and Vav1 was inhibited; however, the state of LAT phosphorylation in these cells was not reported (40). In LAT-deficient mast cells (48), phosphorylation of Vav1 was normal, whereas that of SLP-76, PLCgamma 1, and PLCgamma 2 was inhibited, and calcium responses were also decreased. Thus, it appears that failure to activate Vav1 and/or to target activated Vav1 to a LAT-organized signaling complex (1), where it activates Rac1, contributes to stable activation of PI3K, and promotes phosphorylation and activation of PLCgamma 1 and PLCgamma 2, is a reasonable explanation for the calcium response defect in LAT-, SLP-76-, or Vav1-deficient cells (40, 48). Our present findings strongly support this premise, because, in the absence of Vav1, association of PLCgamma 1, PLCgamma 2, and SLP-76 with LAT was not significantly altered. Thus, all components (with the exception of Vav1) shown to regulate calcium responses localized appropriately, but Vav-/- cells showed transient PI3K activation, loss of PLCgamma 1 and PLCgamma 2 phosphorylation, reduced IP3 levels, and diminished calcium responses. Furthermore, rescue of the defective tyrosine phosphorylation of both PLCgamma isoforms and calcium responses was achieved by expression of Vav1. Thus, we conclude that Vav1 association with a LAT-organized complex regulates the activity of PLCgamma 1 and PLCgamma 2 in membranes.

Interestingly, in CD3/CD28-stimulated Vav-/- T cells PLCgamma 1 phosphorylation appeared normal, although IP3 calcium responses were reduced (13). The diminished IP3 response suggests that activation or function of PLCgamma is defective in Vav1-deficient T cells; thus, we have no clear explanation for the seemingly normal phosphorylation of Vav1 in T cells. Nevertheless, it is possible that Vav1 functions differently in T cells than in mast cells or that it regulates PLCgamma activity by other means in these cells. Our data show that Vav1 contributes to prolonged PI3K activation and thus to the presence of the PI3K product, PIP3, which regulates the membrane recruitment and activity of PLCgamma (43). Thus, the absence of Vav1 could inhibit calcium mobilization by affecting the activity of PI3K and thus the recruitment or, more likely (based on our findings of normal association with LAT), the activation of PLCgamma , consistent with the previously described role of PI3K in calcium responses (3). Our finding that thapsigargin did not reconstitute calcium responses in Vav1-deficient mast cells also supports a connection between Vav1 and PI3K, as the thapsigargin-induced calcium response was previously demonstrated to be PI3K dependent in mast cells (11, 25). Interestingly, the GEF activity of Vav1 was found to be critical for Vav1-mediated calcium responses and for the tyrosine phosphorylation of PLCgamma 2 but less essential for PLCgamma 1 phosphorylation. Therefore, because wild-type Vav1 reconstitutes the phosphorylation of both PLCgamma isoforms, our findings support the view that Vav1 has both GEF-dependent and -independent functions (33) and suggest that both functions may contribute to the calcium response. Because of Vav's multiple protein-protein interaction domains, it is not surprising that Vav1 may facilitate the juxtaposition of a (as yet unidentified) tyrosine kinase with its substrate, namely, PLCgamma 1, resulting in its phosphorylation independent of the Vav1 GEF activity. Moreover, preliminary experiments support a role for Vav's adapter function in the regulation of the calcium response, as Vav-/- mast cells reconstituted with an SH2 domain-mutated Vav1 (21) showed a dramatic delay in calcium responses (data not shown). Nevertheless, our results show that the apparently normal phosphorylation of PLCgamma 1 alone is not sufficient for the calcium response and that the GEF activity of Vav1 is required. In fact, recent studies demonstrate that the Rho family members Rac1 and Cdc42 serve as regulators of mast cell degranulation by activating the IP3-calcium pathway (23). Additionally, these small GTPases can reconstitute calcium responses and degranulation in a response-deficient RBL mast cell line (24). Since Vav1 is an upstream effector controlling Rac1 and possibly Cdc42 activation (15, 38), and these small GTPases are important in PI3K activation (30), Vav1's adapter and GEF activities appear to function synergistically to regulate PLCgamma 1 and PLCgamma 2 activity and calcium responses.

The prominent phenotype of Vav1-deficient mice is a defect in the development of T cells and the B1 B-cell subset. The present study of normally developing mast cells provides the suggestion that Vav-1-mediated PLCgamma activation may be involved in ontogeny of T cells and of certain B cells. This is supported by the recent observation that PLCgamma 2-deficient mice demonstrated a selective absence of the B1 B-cell subset (59) akin to the defect seen in Vav1-/- mice (63). However, PLCgamma 2-deficient mice showed normal T-cell development; thus, PLCgamma 2 does not appear to play a critical role in T-cell development (59). Whether PLCgamma 1 activation plays a role in T-cell development remains to be determined, but PLCgamma 1 has been demonstrated elsewhere to play an important role in mammalian growth and development, as homozygous deletion of this gene results in embryonic lethality (28). It is clear, however, that development of different cell types is variably dependent on activation of PLCgamma and calcium responses (28, 49, 59).

Our findings demonstrate Vav1's ability to activate PLCgamma by phosphorylation- and GEF-dependent pathways as critical for normal calcium responses in mast cells. Vav1 is selectively expressed in hematopoietic cells, but other homologs are ubiquitously expressed and can serve as GEFs for members of the Rho family of GTPases (6). Thus, beyond their role as GEFs in the remodeling of the cell's cytoskeleton, it remains to be determined whether all members of this GEF family function in the regulation of calcium signals and whether the mechanisms involved are common to all members.


    ACKNOWLEDGMENT

This work was partly supported by a Pan American Fellowship award (CONACYT/NIH) to C.G.-E.


    FOOTNOTES

* Corresponding author. Mailing address: NIAMS/NIH, Building 10, Room 9N228, 10 Center Dr., MSC 1820, Bethesda, MD 20892-1820. Phone: (301) 496-7592. Fax: (301) 402-0012. E-mail: juan_rivera{at}nih.gov.


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Abstract
Introduction
Materials and Methods
Results
Discussion
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Molecular and Cellular Biology, June 2001, p. 3763-3774, Vol. 21, No. 11
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.11.3763-3774.2001


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