INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

The human c-fes proto-oncogene encodes a 93-kDa non-receptor protein tyrosine kinase (Fes) with strong expression in myeloid hematopoietic cells. Fes has been implicated in signal transduction pathways for several hematopoietic cytokines, including interleukin-3 (IL-3), IL-4, IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and erythropoietin (reviewed in reference 37). Other evidence suggests that Fes may contribute to differentiation signaling from cytokine receptors. For example, expression of Fes in the human myeloid leukemia cell line K-562 results in growth suppression and terminal differentiation (4, 40). More recent work suggests that interaction with Bcr-Abl may be responsible for Fes activation in this chronic myelogenous leukemia (CML)-derived cell line, implicating Fes as a suppressor of CML progression as well (19). On the other hand, inhibition of Fes expression with antisense oligonucleotides blocks differentiation of HL-60 cells and triggers an apoptotic response instead (24). These results implicate Fes in both differentiation and survival signaling pathways in myeloid leukocytes.

The c-Fes protein consists of three distinct structural domains: a long unique N-terminal region, a central SH2 domain, and a C-terminal kinase domain (37). Fes lacks an SH3 domain, a negative regulatory tail tyrosine residue, and other structural features that contribute to the negative regulation of Src, Abl, and other non-receptor protein tyrosine kinases. However, like Src and Abl, Fes tyrosine kinase activity is tightly regulated in mammalian cells and induces little or no transforming activity upon ectopic expression in rodent fibroblasts (6, 8). The mechanism responsible for negative regulation of Fes tyrosine kinase activity in vivo is not clear.

One unique aspect of c-Fes structure is the presence of coiled-coil homology domains in the unique N-terminal region (4, 33). Coiled coils are composed of amphipathic -helices that exhibit a heptad repeat pattern in which the first and fourth positions are occupied by hydrophobic amino acids (21). These residues align to form a hydrophobic interface between the supercoiled strands. Previous work from our laboratory has shown that the N-terminal region of Fes mediates its oligomerization in vitro, suggesting that interconversion of monomeric and oligomeric forms of the protein may regulate kinase activity in vivo. Consistent with this idea is our finding that overexpression of the isolated Fes N-terminal region can suppress the kinase and transforming activities of a full-length Fes mutant protein that is activated by membrane targeting. The suppressive effect may involve formation of an inactive heteromer comprised of full-length and truncated strands (4, 33).

In this study, we provide evidence that the Fes coiled-coil homology domains are required for both negative regulation and full activation of kinase function. We observed that a single point mutation of a key leucine residue in the first coiled-coil (CC1) domain results in strong kinase activation in vivo, leading to potent transforming activity in fibroblasts as well as survival and differentiation signaling in myeloid cells. Interestingly, when the activating mutation in CC1 is combined with a similar point mutation in the second coiled coil (CC2), tyrosine kinase and biological activities are significantly impaired, suggesting that CC2 may be required for mediating Fes oligomerization or downstream signaling in vivo. Gel filtration experiments show that the unique N-terminal region can adopt monomeric and oligomeric forms, suggesting that the coiled coils regulate kinase activity by controlling the oligomerization state of the protein. Use of coiled-coil domains is unique among non-receptor protein tyrosine kinases as a regulatory mechanism.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Construction of Fes coiled-coil point mutant proteins. Residues essential for coiled-coil structure were determined using the COILS algorithm (23) (see Fig. 1) and include Leu 145 in the CC1 domain and Leu 334 in the CC2 domain. These leucine residues were converted to proline using an Altered Sites mutagenesis kit according to the instructions of the manufacturer (Promega). Both individual-mutation (L145P, L334P) and double-mutation (2LP) proteins were created. The presence of each mutation was confirmed by automated DNA sequence analysis.

Construction of GFP-Fes fusion proteins. To create green fluorescent protein (GFP)-Fes expression constructs, the enhanced GFP coding sequence was amplified from the vector pEGFP-C1 (Clontech) by PCR and subcloned into the retroviral expression vector pSRMSVtkneo (27) to create plasmid pSR-GFP. The cDNA clones encoding wild-type Fes, the coiled-coil domain point mutant proteins, and the other forms of Fes used in this study were subcloned downstream and in frame with GFP in this vector.

Production of recombinant retroviruses. Recombinant retroviruses were produced by cotransfecting 293T cells with the pSR-GFP-Fes constructs described above and either ecotropic or amphotropic packaging vectors as described elsewhere (2, 4, 18). A GFP retrovirus was prepared from the pSR-GFP parent vector for use as a negative control. Retroviral supernatants were collected every 12 h for 3 days, and the pooled supernatants were aliquoted and stored at 80°C.

Retroviral infection of Rat-2 fibroblasts and focus-forming assay. Rat-2 cells were obtained from the American Type Culture Collection and maintained in Dulbecco's modified Eagle's medium supplemented with 5% fetal bovine serum. Cells were plated in six-well plates (2 × 10⁵ cells per well) 1 day prior to infection. Retroviral stocks (4 ml) were thawed on ice, supplemented with Polybrene to 4 µg/ml, and added to the cells. The plates were centrifuged at 1,500 × g for 3 h at room temperature to enhance the efficiency of infection. The virus was replaced with fresh medium, and the infected cells were incubated for 48 h at 37°C prior to being plated in transformation assays.

Expression of Fes coiled-coil mutant proteins in TF-1 myeloid leukemia cells. The human myeloid leukemia cell line TF-1 (17) was obtained from the American Type Culture Collection and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 50 µg of gentamicin per ml, and 1 ng of GM-CSF (BioSource International) per ml. Recombinant retroviruses for TF-1 cell infection were prepared using the 293T cell protocol described above, except that an amphotropic packaging plasmid was used. For TF-1 cell infection, 2 × 10⁵ cells were plated in each well of a six-well plate and centrifuged at 500 × g for 15 min. The medium was aspirated and replaced with the retroviral supernatant plus Polybrene, and the infection protocol was continued as described above for the Rat-2 cells. Following infection, the cells were placed under G418 selection for 10 to 14 days. Expression of GFP or the GFP-Fes fusion proteins was confirmed by fluorescence microscopy, under which the drug-resistant cell population exhibited uniform green fluorescence. Expression of full-length GFP-Fes fusion proteins was also confirmed by immunoblotting (see below).

Analysis of Fes protein expression and autophosphorylation. Aliquots of Rat-2 or TF-1 cells were pelleted, washed with phosphate-buffered saline, and lysed in 1 ml of radioimmunoprecipitation assay (RIPA), buffer (50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulfate [SDS], 1 mM EDTA, and 1% sodium deoxycholate). Fes proteins were immunoprecipitated from clarified cell lysates using either the M2 anti-FLAG monoclonal antibody resin (Sigma) or an anti-GFP antibody (Clontech) and protein G-Sepharose (Pharmacia). Immunoprecipitates were washed with RIPA buffer, resolved by SDS-polyacrylamide gel electrophoresis (PAGE), and immunoblotted for Fes expression with the anti-FLAG antibody or for phosphotyrosine content with the antiphosphotyrosine monoclonal antibody PY99 (Santa Cruz).

Gel filtration analysis of the Fes N-terminal region. The coding sequence of the Fes N-terminal region encompassing both coiled-coil motifs (Ala 91 to Gly 392; CC1+2) was amplified from wild-type and coiled-coil mutant Fes templates by PCR and subcloned into the baculovirus transfer vector pVL-GFP. The pVL-GFP vector was constructed by PCR amplification of the enhanced GFP coding sequence from pEGFP-C1 (Clontech) and subcloning downstream of the polyhedrin promoter. The resulting vectors were used to produce recombinant baculoviruses using Baculogold DNA (PharMingen) and the manufacturer's protocol. For gel filtration experiments, the GFP-Fes CC1+2 fusion proteins were expressed in Sf-9 insect cells and lysates were prepared by sonication in 1.0 ml of S-300 buffer (50 mM Tris-HCl [pH 7.4], 50 mM NaCl, 1 mM EDTA). Lysates were clarified by centrifugation at 100,000 × g for 1 h and applied to a Sephacryl S-300 column (1.6 by 77 cm) previously equilibrated with S-300 buffer. Fractions (0.25 ml) were collected directly in 96-well microtiter plates, and the elution profile of each GFP-CC1+2 fusion protein was determined by fluorescence detection using a SpectraMax Gemini XS fluorimetric microplate reader (Molecular Devices, Inc.). As an additional control, unfused GFP was run alone and found to be monomeric. Peak fractions were pooled and concentrated, and the presence of the GFP-CC1+2 fusion protein was verified by immunoblotting with anti-GFP antibodies (Clontech).

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Identification of N-terminal residues critical for coiled-coil structure. Coiled-coil domains are characterized by a heptad repeat of amino acids often denoted a-b-c-d-e-f-g, with hydrophobic residues at positions a and d (21). The hydrophobic residues pack against each other to form the supercoil interface, while polar residues generally occupy the other positions and interact with solvent. Analysis of the Fes sequence with the COILS algorithm (22) predicts two regions with a high probability of coiled-coil structure formation within the Fes N-terminal region (33) (Fig. 1). Using COILS, we identified Leu residues that occupy a central d position within each predicted coiled coil and appear to be critical for the integrity of the coiled-coil structure. As shown in Fig. 1, replacement of Leu 145 with Pro in the first coiled-coil motif reduced the probability of coiled-coil formation from nearly 100% to less than 40%. A similar Pro substitution for Leu 334 within the second coiled-coil region nearly abolished the predicted coiled-coil formation. These substitutions were introduced into the full-length c-Fes coding region either alone or in combination. The proteins with the resulting coiled-coil mutations (L145P, L334P, and 2LP) were fused to GFP in a retroviral vector for expression in fibroblasts and human myeloid progenitor cells. The structures of these Fes proteins and the other constructs used in this study are shown in Fig. 2.

View larger version (19K): [in this window] [in a new window]   FIG. 1.   Identification of Fes N-terminal residues essential for coiled-coil formation. Wild-type and mutant forms of the 822-amino-acid Fes sequence were analyzed using the COILS algorithm. The probability of each residue contributing to coiled-coil formation is plotted as a function of its position within the overall sequence. The mutant proteins include one with a proline substitution for Leu 145 in the CC1 domain (L145P) protein, one with a proline substitution for Leu 334 in the CC2 domain (L334P protein), and a double mutant protein with both substitutions (2LP). For this analysis, a 28-residue sliding window was used along with the MTIDK matrix. For additional details, see http://www.ch.embnet.org/software/COILS_form.html. WT, wild type.

View larger version (26K): [in this window] [in a new window]   FIG. 2.   GFP-Fes constructs used in this study. The structure of the wild-type GFP-Fes fusion protein is shown at the top and includes a unique N-terminal region, an SH2 domain, and a C-terminal kinase domain. The locations of the two coiled-coil homology regions are indicated as the shaded boxes (CC1 and CC2). Mutant proteins include one with a proline substitution for Leu 145 in the first coiled-coil domain (L145P), one with a proline substitution for Leu 334 in the CC2 domain (L334P), and the corresponding double mutant protein with both substitutions (2LP). We also constructed GFP fusion proteins of the CC1 and CC2 deletion mutant proteins and the corresponding kinase-inactive forms of these proteins (CC1-KE, CC2-KE). All constructs have a C-terminal FLAG epitope tag (not shown).

Proline substitution for d-position leucine residues in the Fes coiled-coil domains have opposing actions on tyrosine kinase activity in Rat-2 fibroblasts. Previous studies have shown that wild-type c-Fes is nontransforming and that its tyrosine kinase activity is highly repressed following expression in Rat-2 fibroblasts, providing a convenient system to study the contribution of Fes structural features to the negative regulation of kinase activity (4, 8). Using recombinant retroviruses, the Fes coiled-coil domain point mutant proteins were expressed in Rat-2 fibroblasts as GFP fusion proteins. The GFP-Fes proteins were immunoprecipitated, and kinase activity was analyzed in terms of autophosphorylation by antiphosphotyrosine immunoblotting. As shown in Fig. 3, wild-type GFP-Fes tyrosine autophosphorylation was very low in this system, indicative of negative regulation as observed previously. However, proline substitution for Leu 145 within the CC1 domain enhanced autophosphorylation by more than 10-fold, indicating that the CC1 domain is required for negative regulation of kinase activity in vivo. Proline substitution for Leu 334 in the CC2 domain did not affect kinase activity relative to that of wild-type GFP-Fes. However, this same substitution reduced the kinase activity of the L145P mutant protein by more than 50%, suggesting that an intact CC2 domain is required for efficient autophosphorylation in vivo.

View larger version (33K): [in this window] [in a new window]   FIG. 3.   Autophosphorylation of GFP-Fes proteins in Rat-2 fibroblasts. Rat-2 fibroblasts stably expressing each of the GFP-Fes constructs shown in Fig. 2 were lysed, and GFP-Fes fusion proteins were precipitated with the M2 anti-FLAG monoclonal antibody. (A) Immunoprecipitates were washed with RIPA buffer, resolved by SDS-PAGE, and immunoblotted for Fes expression with the anti-FLAG antibody (Fes) or for phosphotyrosine content with the antiphosphotyrosine monoclonal antibody PY99 (P-Tyr). A representative blot is shown. (B) The experiment was repeated three times, and the relative levels of GFP-Fes protein and phosphotyrosine content were quantitated using an imaging densitometer. Ratios of tyrosine autophosphorylation to Fes protein were calculated and are presented as means ± standard deviations. KE, kinase-inactive form.

The Fes CC1 domain point mutant protein exhibits strong transforming activity in Rat-2 cells. Results presented in the previous section show that disruption of the CC1 domain with a Leu-to-Pro substitution releases Fes kinase activity in vivo. To determine whether this mutant protein retains the capacity to generate a biological signal, we compared its transforming activity with those of wild-type Fes and the other coiled-coil domain mutant proteins. Rat-2 fibroblasts were infected with recombinant Fes retroviruses and placed under G418 selection for 2 weeks, at which time the foci were stained and counted. As shown in Fig. 4 and 5, the wild-type GFP-Fes fusion protein did not exhibit focus-forming activity, consistent with earlier studies of wild-type c-Fes (4, 8, 18). However, the L145P mutant protein exhibited very potent focus-forming activity, consistent with the effect of this mutation on Fes autophosphorylation. In contrast, proline substitution for Leu 334 in the CC2 domain did not release transforming activity. Combining the CC1 and CC2 mutations reduced focus-forming activity by more than 50% relative to that of the L145P single mutant protein. The focus-forming activities of the mutant proteins closely paralleled the relative levels of kinase activity (Fig. 3).

View larger version (84K): [in this window] [in a new window]   FIG. 4.   Focus-forming activity of GFP-Fes proteins in Rat-2 fibroblasts. Rat-2 fibroblasts were infected with recombinant GFP-Fes retroviruses as described in Materials and Methods. For focus-forming assays, infected cells were plated in 60-mm-diameter dishes and incubated in the presence of G418 for 14 days. Scanned images of the stained cultures are shown, which appear as confluent monolayers with transformed foci (where present) growing on top.

View larger version (26K): [in this window] [in a new window]   FIG. 5.   Quantitative comparison of GFP-Fes coiled-coil mutant protein transforming activities. Each of the GFP-Fes proteins illustrated in Fig. 2 was tested for focus-forming activity as described in Materials and Methods and the legend to Fig. 4. Foci were visualized by Wright-Giemsa staining and counted using a Bio-Rad model GS-710 scanning densitometer and colony-counting software. Foci from three independent cultures were counted; the bar graph shows average values ± standard deviations. KE, kinase-inactive form.

The Fes CC1 domain point mutant protein generates signals for survival and attachment in myeloid leukemia cells. Previous studies have suggested that Fes may transduce signals for differentiation and survival in response to cytokines or other upstream signals (37). To determine whether the Fes CC1 domain point mutant protein can generate signals in a physiological context, we expressed this mutant protein in the myeloid leukemia cell line TF-1. This cell line requires GM-CSF for growth and differentiates into macrophage-like cells in response to phorbol esters (17). Wild-type GFP-Fes as well as each of the coiled-coil domain mutant proteins were introduced into TF-1 cells using recombinant retroviruses along with GFP alone as a negative control. Infected cell populations were selected in the presence of G418 and GM-CSF, and uniform expression of the transduced proteins in each of the infected cell populations was verified by fluorescence microscopy (data not shown). To determine whether expression of the Fes coiled-coil point mutant proteins affected the requirement of TF-1 cells for cytokine, levels of growth of the infected cell populations were compared in the presence and absence of GM-CSF. As shown in Fig. 6, no proliferation was observed in cells expressing wild-type GFP-Fes or GFP alone. However, cells expressing the Fes protein with the CC1 domain point mutation (L145P) grew rapidly in the absence of cytokine, providing direct evidence that Fes kinase activity is sufficient to generate a signal for survival and proliferation in myeloid cells. Interestingly, the protein with point mutations in both CC domains was unable to generate this response, suggesting that similar mechanisms regulate Fes activity in myeloid cells and upon ectopic expression in fibroblasts. All of the cell populations grew rapidly in the presence of GM-CSF. The protein with the CC2 domain point mutation (L334P) was unable to support cytokine-independent proliferation of TF-1 cells, consistent with the fibroblast result (data not shown).

View larger version (24K): [in this window] [in a new window]   FIG. 6.   Cytokine-independent survival and proliferation of TF-1 myeloid leukemia cells expressing the GFP-Fes CC1 domain point mutant protein. TF-1 cells were infected with recombinant retroviruses carrying GFP as a negative control (open bars), GFP-Fes (gray bars), GFP-Fes L145P protein (hatched bars), or GFP-Fes 2LP (solid bars). Following infection, the cells were placed under G418 selection in the presence of GM-CSF for 10 to 14 days, at which point each of the drug-resistant cell populations exhibited uniform green fluorescence (data not shown). To monitor GM-CSF dependence, cells were washed free of cytokine and replated in the absence (top panel) or presence (bottom panel) of GM-CSF and viable cells were counted following trypan blue staining every 2 days. Two independent experiments yielded comparable results.

View larger version (53K): [in this window] [in a new window]   FIG. 7.   A point mutation in the GFP-Fes CC1 domain induces attachment and spreading of TF-1 myeloid leukemia cells. TF-1 cells were infected with a recombinant GFP retrovirus or the indicated GFP-Fes retroviruses and selected as described in the legend to Fig. 6. Following selection, the cells were replated in 24-well plates in the presence of GM-CSF and returned to the incubator for 5 days. Cells were stained in situ with Giemsa stain and visualized by light microscopy. Phase-contrast images of TF-1 cells expressing GFP, GFP-Fes, GFP-Fes L334P, and GFP-2LP were recorded under low-power magnification (×100). Images of cells expressing the GFP-Fes CC1 domain point mutant (L145P) protein were recorded under low-power (middle left panel) and high-power (×400; middle right panel) magnification.

View larger version (17K): [in this window] [in a new window]   FIG. 8.   Quantitative comparison of the effects of GFP-Fes CC domain mutant proteins on TF-1 cell morphology in the presence and absence of GM-CSF. TF-1 cells were infected with a recombinant GFP retrovirus or the indicated GFP-Fes retroviruses and selected as described in the legend to Fig. 6. For attachment experiments, 5 × 104 cells were plated in duplicate 24-well plates in the presence (+) or absence () of GM-CSF. Cells were returned to the incubator for 5 days (open bars) or 7 days (shaded bars), stained with Giemsa stain, and visualized by light microscopy. The number of attached cells was counted in three randomly chosen fields, and results are presented as average values ± standard deviations. This entire experiment was performed twice and produced the same pattern of responses in each case.

View larger version (25K): [in this window] [in a new window]   FIG. 9.   Autophosphorylation of GFP-Fes CC domain mutant proteins in TF-1 myeloid cells. TF-1 cells stably expressing each of the GFP-Fes fusion proteins indicated were washed free of GM-CSF and incubated in the presence or absence of cytokine for 48 h. Cells were collected by centrifugation and lysed, and GFP-Fes fusion proteins were precipitated with the M2 anti-FLAG monoclonal antibody. Immunoprecipitates were washed with RIPA buffer, resolved by SDS-PAGE, and immunoblotted for Fes expression with the anti-FLAG antibody (Fes) or for phosphotyrosine content with the antiphosphotyrosine monoclonal antibody PY99 (P-Tyr). This experiment was repeated twice and produced comparable results in each case; a representative blot is shown.

Analysis of the wild-type and mutant Fes coiled-coil regions by gel filtration. Data presented here and in our previous studies indicate that the Fes N-terminal coiled-coil domains may control Fes kinase activity by regulating the oligomerization state of the protein (4, 33). To investigate the effects of the coiled-coil domain point mutations on N-terminal oligomerization, we performed a series of gel filtration experiments. For these experiments, wild-type and mutant Fes N-terminal sequences encompassing both coiled-coil motifs (Ala 91 to Gly 392) were expressed as GFP fusion proteins in Sf-9 insect cells. Each GFP-CC1+2 fusion protein was then resolved on a Sephacryl S-300 gel filtration column, and 288 fractions were collected for each sample in 96-well plates. The positions of the GFP-CC1+2 fusion proteins in the elution profiles were determined as the relative fluorescence intensity of each well. As shown in Fig. 10, the wild-type GFP-CC1+2 fusion protein eluted as a mixture of monomeric and oligomeric forms. The peak intensities of the monomer (peak 1) and major oligomer (peak 3) were approximately equal. The monomer (peak 1) eluted with an apparent molecular mass lower than expected (40 kDa observed versus 52 kDa calculated), which may reflect a tight conformation of the protein. However, SDS-PAGE shows that this peak is full-length GFP-CC1+2 (see below). The major oligomer (peak 3) eluted with a mass of approximately 300 kDa, consistent with at least a pentamer; a similar result was observed previously for full-length Fes as well as the complete N-terminal region (33). At least one additional intermediate form was apparent (peak 2), suggesting that Fes can form multiple oligomeric structures. This result provides the first evidence that the Fes coiled-coil region can exist as a monomer and suggests that it can interconvert between monomeric and oligomeric states. Note that GFP itself elutes as a single symmetrical peak, consistent with a monomer, indicating that it does not contribute to oligomer formation (data not shown). The position of GFP in the chromatogram is indicated at the top of Fig. 10.

View larger version (14K): [in this window] [in a new window]   FIG. 10.   Analysis of N-terminal-Fes-GFP fusion proteins by gel filtration. Recombinant fusion proteins of GFP and Fes N-terminal amino acids encoding the CC1 and CC2 domains as well as the intervening sequence were expressed in Sf-9 cells. Cells were lysed by sonication, and lysates were clarified by ultracentrifugation and applied to a Sephacryl S-300 column. Fractions (0.25 ml) were collected in 96-well plates and analyzed for fluorescence using a microplate reader. Four fusion proteins were examined, and the resulting fluorescence elution profiles are shown together with an illustration of each protein. From top to bottom these include wild-type GFP-Fes CC1+2, the CC1 point mutant (L145P) protein, the CC2 point mutant (L334P) protein, and the double mutant protein. The void volume was determined independently using blue dextran, which eluted as a single peak at the position indicated as Vo (fraction 134). All fractions that appeared before the blue dextran peak were devoid of fluorescence and are omitted for clarity. Unfused GFP was also run as a control; it eluted as a single symmetrical peak, the position of which is indicated at the top (fraction 242). The following standards, with their molecular masses and elution positions noted, were also run: thyroglobulin, 634 kDa, fraction 149; ferritin, 439 kDa, fraction 165; catalase, 200 kDa, fraction 185; aldolase, 178 kDa, fraction 189; albumin, 62 kDa, fraction 202; ovalbumin, 48 kDa, fraction 207; and chymotrypsinogen A, 21 kDa, fraction 246. The entire experiment was repeated twice and produced the same pattern of fluorescent peaks for each protein. Note that the absolute levels of fluorescence varied less than twofold among the four fusion proteins.

View larger version (21K): [in this window] [in a new window]   FIG. 11.   Immunoblot analysis of GFP-Fes CC1+2 fusion proteins following gel filtration. Peak fractions from Sephacryl S-300 chromatography of the GFP-Fes CC1+2 fusion proteins shown in Fig. 10 were pooled, concentrated, and resolved by SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes and probed with an antibody to GFP. Results are shown for wild-type GFP-Fes CC1+2 (WT), the CC1 mutation (L145P) protein, the CC2 mutation (L334P) protein, and the double mutant protein (2LP). The numbers above each lane refer to the major peaks shown in Fig. 10.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Cooperation of the CC1 and CC2 domains in the regulation of c-Fes tyrosine kinase activity. Previous studies have shown that c-Fes and the closely related tyrosine kinase Fer exhibit several motifs with strong coiled-coil homology in their unique N-terminal regions (5, 16, 33). In c-Fes, the N-terminal region has been shown to mediate oligomerization of the kinase, leading to autophosphorylation by a trans mechanism (33, 35). These findings suggest that Fes oligomerization may be required for autophosphorylation of Tyr 713 within the activation loop, a prerequisite for kinase activation both in vitro and vivo (13, 35). In this report, we provide evidence demonstrating opposing actions of the coiled-coil domains in the regulation of Fes tyrosine kinase activity in vivo. Proline substitution for Leu 145 in the more N-terminal coiled-coil domain released Fes tyrosine kinase activity in Rat-2 fibroblasts, leading to transformation. This result suggests that the CC1 domain primarily serves a negative regulatory function, a conclusion consistent with our earlier work demonstrating that CC1 deletion can enhance the transforming and tyrosine kinase activities of a membrane-targeted form of c-Fes (4). The activating effect of the L145P mutation was reversed when combined with a proline substitution for Leu 334 in the more C-terminal region of coiled-coil homology. This result indicates that the CC2 domain is required for full kinase activation. Given its close proximity to the SH2 and kinase domains, the CC2 domain may act to align the kinase domains in an orientation favoring efficient trans phosphorylation within the active oligomer. Interestingly, deletion of the CC2 domain within GFP-Fes resulted in a small increase in kinase activity in fibroblasts, suggesting that the CC1 domain may partially complement the loss of CC2 (Fig. 3).

Fes activation is sufficient for survival and differentiation signaling in myeloid cells. Identification of an activated mutant enabled us to investigate the signaling function of Fes in a physiologically relevant cell type. The cell line used for this study was TF-1, a myeloid leukemia cell line that retains a cytokine requirement for growth and survival (17). Expression of the L145P mutant protein of Fes resulted in cytokine-independent proliferation of TF-1 cells, providing evidence that Fes may activate downstream signaling pathways that regulate cellular survival. These results are consistent with earlier work using v-Fps, a transforming retroviral homolog of c-Fes, and other tyrosine kinase oncogenes (25, 30). In addition, constitutive activation of Fes as a result of the L145P mutation caused attachment and spreading of TF-1 cells, consistent with previous work implicating Fes in the terminal differentiation of myeloid leukemia cells (4, 34, 40). Both the survival and differentiation effects of the L145P mutation were almost completely reversed in the double mutant protein (2LP), suggesting that an intact CC2 domain is required for efficient kinase activation or downstream signaling in myeloid cell types. Interestingly, the CC2 mutation did not have as dramatic an impact on the transforming function of the GFP-Fes L145P protein following ectopic expression in Rat-2 cells. One implication of this difference is that CC2-mediated interaction of Fes with a specific signaling partner may be required for the generation of physiological signals in myeloid cells. It also possible that cell-specific trans-acting factors may impose more strict control on Fes kinase activity in physiological sites of expression.