INTRODUCTION

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Protein synthesis in intact reticulocytes and their lysates is dependent on the availability of heme. In heme deficiency, protein synthesis is inhibited, with a marked decrease in the formation of 40S-eukaryotic initiation factor 2 (eIF2)-Met-tRNA_f GTP (43S preinitiation complex) and with increased phosphorylation of the  subunit of eIF2. eIF2 is an initiation factor which binds GTP and Met-tRNA_f and is required for the formation of the 43S initiation complex. The phosphorylation of eIF2 in heme deficiency is the result of the activation of heme-regulated inhibitor (HRI), which is a heme-regulated eIF2 kinase (8, 9). The mechanism of inhibition of translation initiation by the phosphorylation of eIF2 has been studied extensively (18, 19). In brief, the recycling of eIF2 in initiation requires the exchange of bound GDP for GTP. Under physiological conditions, eIF2 has a 400-fold-greater affinity for GDP than for GTP. The exchange of tightly bound GDP for GTP requires eIF2B, which is rate limiting and is present at 15 to 25% of the amount of eIF2. When eIF2 is phosphorylated, the binding of eIF2(P)-GDP to the regulatory subcomplex of eIF2B is much tighter than the binding of eIF2-GDP to eIF2B (25). This tighter interaction of phosphorylated eIF2 with eIF2B prevents the GDP-GTP exchange activity of eIF2B. In this manner, once the amount of phosphorylated eIF2 exceeds the amount of eIF2B, the shutoff of protein synthesis occurs.

Phosphorylation of eIF2 was later found to be a common regulatory mechanism of protein translation under various stress conditions and is carried out by the family of eIF2 kinases. In addition to HRI, there are three other known eIF2 kinases at present. They are the interferon-induced double-stranded RNA (dsRNA)-dependent eIF2 kinase (PKR), the GCN2 protein kinase, and the recently identified mammalian endoplasmic reticulum (ER) resident kinase (PERK), which is identical to PEK that is highly expressed in the pancreas (17, 38). These eIF2 kinases share extensive homology in their kinase catalytic domains and phosphorylate eIF2 at the serine 51 residue (reviewed in reference 23). However, the regulatory domains and the regulatory mechanism of each of these eIF2 kinases are very different. PKR is regulated by dsRNA through two N-terminal dsRNA-binding domains and is activated by dsRNA upon viral infection (reviewed in reference 33). GCN2 is activated under the condition of amino acid starvation through the C-terminal domain, which contains a His-tRNA synthase-like sequence (3, 20, 40). PERK is essential for ER stress and is activated by ER stress through its luminal domain, which is similar to the sensor domain of the ER-stress kinase Irel (16, 17, 38). HRI is regulated by heme through the two heme-binding domains in the N terminus and kinase insert domain (6, 34).

In addition to the stress conditions described above, there are other environmental stresses that are known to induce rapid inhibition of translation in the cell through eIF2 phosphorylation. These include heat shock, arsenite treatment, osmotic stress, and nutrient starvation (4). It remains to be determined which eIF2 kinase(s) is activated under these stresses. Recently, the availabilities of cells lacking PKR or PERK have been useful in addressing this question. It has been shown that PERK is essential for ER stress but not for cytoplasmic stresses such as arsenite or heat shock (16, 17). PKR is dispensable for either essential amino acid starvation or ER stress (17, 24).

The production of HRI knockout mice in our laboratory (14) enables us to determine the role of HRI in these stress responses. This work was performed using mouse reticulocytes and nucleated erythroid progenitor cells from fetal livers. Since HRI is most abundant in erythroid cells, these cells are most suitable for studying the functions of HRI. Furthermore, reticulocytes have been used classically as a model system for studying translation, since they have no nuclei and therefore no transcriptional regulation, which can also affect protein output.

We report here, for the first time, the presence of HRI in nucleated erythroid progenitor cells. In addition, in both reticulocytes and fetal liver erythroid progenitor cells, HRI is activated by various cytoplasmic stresses, including arsenite treatment, heat shock, and osmotic shock, but not by ER stress or nutrition starvation. Most importantly, our results demonstrate that HRI is the only eIF2 kinase that is activated by arsenite treatment of erythroid cells, as HRI^/ cells fail to increase eIF2 phosphorylation upon arsenite treatment. These results underscore the importance of HRI in stresses other than heme deficiency, particularly the oxidative stress induced by arsenite.

	MATERIALS AND METHODS

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Materials. [³⁵S]methionine (3,000 Ci/mmol) and [-³²P]ATP (3,000 Ci/mmol) were purchased from New England Technologies Inc. Sodium arsenite, N-acetyl-L-cysteine (NAC), and clofibric acid (CIA) were from Sigma, and geldanamycin (GA) was from Calbiochem. The anti-PKR antibody was from Santa Cruz, and the anti-phosphorylated-eIF2 antibody was from Biosource International Inc.

Preparation of mouse reticulocytes. Wild-type, HRI knockout, and PKR knockout mice were injected with phenylhydrazine at 40 mg/kg on days 0, 1, and 3 to induce hemolysis, subsequent erythropoiesis, and release of reticulocytes into the bloodstream. Blood samples were collected by heart puncture at day 7, and the percentages of reticulocytes in the peripheral blood were determined by new methylene blue staining. The percentages of reticulocytes were between 85 and 95%.

Stress challenge of reticulocytes. The blood samples were washed twice with ice-cold phosphate-buffered saline supplemented with 5 mM glucose and resuspended in Dulbecco modified Eagle medium with 2% dialyzed fetal bovine serum at a concentration of 10⁸ reticulocytes/ml. The cells were plated onto six-well plates (10⁸/well) and preincubated for 30 min at 37°C in a tissue culture incubator for recovery. The cells were then subjected to different stresses: (i) heat shock at 42°C for 30 min, (ii) sodium arsenite at various concentrations and time periods as indicated in the figures, (iii) osmotic shock with 0.5 M NaCl for 1 h, and (iv) Thapsigargin (Tg) (1 to 20 µM) for ER stress for 1 h. For nutrient starvation, the reticulocytes were washed three times with either serum-free or methionine-free medium and maintained in these media for 1 h for serum or amino acid starvation. In examining the chaperone antagonists and reducing reagent, the cells were pretreated with GA (4 to 40 µg/ml), CIA (5 to 40 mM), and NAC (5 to 20 mM) for 30 min before the stress challenges. For protein synthesis, cells were pulse-labeled with 100 µCi of [³⁵S]methionine in low-methionine medium (containing 1/10 of the methionine concentration of the complete medium) for 30 min after the stress challenge.

Preparation and stress challenge of mouse fetal liver cells. Embryonic livers at day 14.5 from both wild-type and HRI^/ mice were excised and dispersed by being passed through a 21-gauge needle in Dulbecco modified Eagle medium with 2% fetal bovine serum. A single-cell suspension was preincubated in this culture medium for recovery for at least 45 min. The cells were used for stress response study as described above for reticulocytes.

Lysate preparation and Western blot analysis. For Western blotting, the cells were lysed in sodium dodecyl sulfate (SDS) sample buffer by adding 1/3 volume of 4× SDS sample buffer directly into cell suspensions. The samples were then boiled for 3 min and loaded onto SDS-polyacrylamide gels (7% for HRI and 12% for eIF2P and total eIF2). Western blot analyses for HRI and eIF2 were carried out as described previously (2).

Immunoprecipitation (IP) and kinase assays. Cells (10⁸) were lysed in 500 µl of cell lysis buffer (20 mM Tris-HCl [pH 7.4], 150 mM NaCl, 1 mM Na₂EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na₃VO₄, 1 mM NaF, 1 µM aprotinin, 1 µM leupeptin, 5 µM pepstatin A, and 1 mM phenylmethylsulfonyl fluoride) at 4°C for 15 min. Cell debris were removed by centrifugation at 10,000 × g for 5 min at 4°C. Cell lysates from equal numbers of cells (10⁷) were mixed with anti-mouse HRI antiserum (14) or anti-PKR polyclonal antibody and incubated at 4°C for 2 h. Protein A-Sepharose beads (50 µl) were then added, and the samples were incubated at 4°C overnight with rocking. The Sepharose immunocomplex was washed five times with 1 ml of wash solution (50 mM Tris-HCl [pH 7.4], 50 mM NaCl, 1 mM Na₂EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na₃VO₄, 1 mM NaF, and protease inhibitors). After the last wash, the Sepharose beads were resuspended in 20 µl of 2× kinase assay buffer (40 mM Tris-HCl [pH 8.0], 4 mM MgCl₂, and 0.2 mM EDTA). eIF2 kinase assays were then performed by the addition of 3.5 µl of labeling mix (0.5 µl of 10 mM ATP, 5 µCi of [-³²P]ATP, and 200 ng of the recombinant yeast eIF2) at 30°C for 5 min for HRI or 20 min for PKR. The reactions were terminated by the addition of 10 µl of 3× SDS sample buffer. The extent of eIF2 phosphorylation was visualized by autoradiography after SDS-polyacrylamide gel electrophoresis (SDS-PAGE).

Tetracycline-inducible expression of HRI in CHO cells. The cDNAs of both the wild type and an inactive mutant of HRI (K196R HRI) were put into the pCDNA4/TO vector with the tetracycline-inducible promoter (Invitrogen). The plasmid DNAs were transfected into the Chinese hamster ovary (CHO)-TRX cell line (Invitrogen), and stable cell lines were established by selection of the cells with both Zeocin and Blasticidin. Expression of HRI and its mutant was induced with 1 µg of tetracycline per ml for 24 h. Cells were then treated with arsenite at 100 µM for 1 h. Cells were washed once with cold phosphate-buffered saline and lysed in SDS sample buffer. The expression and activation of HRI were detected by Western blot analysis as described above.

	RESULTS

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Activation of HRI by arsenite treatment. To characterize the role of HRI in the inhibition of translation in response to arsenite treatment, reticulocytes were produced by treating mice with phenylhydrazine as described in Materials and Methods. Cells were washed, incubated in culture medium, and then treated with various concentrations of sodium arsenite for 60 min or with 200 µM arsenite for different time periods. Protein synthesis was measured by the incorporation of [³⁵S]methionine into the globin chains. Arsenite treatment shut off globin synthesis completely in these cells, since no detectable [³⁵S]methionine incorporation into globins was observed in the arsenite-treated cells (Fig. 1A). Arsenite has been shown to induce eIF2 phosphorylation in NIH 3T3 cells (30). The extent of eIF2 phosphorylation in arsenite-treated reticulocytes was examined by Western blot analysis using anti-phospho-eIF2 antibody. As shown in Fig. 1A, there was a pronounced increase in eIF2 phosphorylation in the reticulocytes upon arsenite treatment, while the amount of total eIF2 remained constant.

View larger version (44K): [in this window] [in a new window]   FIG. 1.   Activation of HRI by arsenite in mouse reticulocytes. (A) Effect of arsenite concentration on activation of HRI. Mouse reticulocytes were isolated as described in Materials and Methods. After recovery, the cells were treated with 100, 200, or 400 µM arsenite (As) or 1 µM Tg for 30 min and then labeled with [35S]methionine for an additional 30 min. Total cellular proteins were then separated by SDS-PAGE. The incorporation of [35S]methionine into globins was detected by exposing the nitrocellulose membrane to X-ray film. The extents of HRI activation and eIF2 phosphorylation were detected with anti-mouse HRI antibody and anti-eIF2P antibody. Total eIF2 was determined with anti-eIF2 monoclonal antibody. The upshifted species of HRI are indicated by an arrow. C, control. (B) Time course of HRI activation by arsenite. Mouse reticulocytes were treated with 200 µM arsenite for different time periods as indicated. (C) Kinase activity of HRI. HRI in reticulocytes was immunoprecipitated from lysates of arsenite-treated, Tg-treated, or control reticulocytes with anti-HRI antibody, and the kinase activities were assayed in vitro by using recombinant yeast eIF2 as a substrate. The incorporation of 32P into eIF2 was detected by autoradiography. In lanes 7 and 8, the reticulocyte lysates from the control cells were either treated in vitro with 50 µM arsenite or heat shocked (hs) at 42°C for 30 min prior to immunoprecipitation. (D) Heme-independent activation of HRI by arsenite. Reticulocytes were treated with or without hemin (H, 40 µM) for 1 h prior to arsenite treatment (200 µM, 1 h).

Activation of HRI by various cytoplasmic stresses in reticulocytes and nucleated erythroid progenitor cells. Since HRI is activated by arsenite but not by Tg, the question whether HRI can also be activated by other cytoplasmic stresses was examined. Reticulocytes and fetal liver erythroid cells were subjected to different stress conditions as described in Materials and Methods. Activation of HRI in reticulocytes detected by electrophoretic mobility shift was observed under heat shock and osmotic stress, with corresponding increases of eIF2 phosphorylation and inhibition of globin synthesis under these conditions (Fig. 2A and B). However, HRI was not activated under amino acid starvation or serum starvation (Fig. 2B and C). The increases in eIF2P in vivo were quantitated from Western blot signals from three separate experiments; there was a three- to fourfold-higher eIF2P level in reticulocytes under these stress conditions (Fig. 2B). Although this method of detection does not allow estimation of the percentage of eIF2 phosphorylated, it is useful and adequate for determining the relative extents of eIF2 phosphorylation under different conditions.

View larger version (45K): [in this window] [in a new window]   FIG. 2.   Differential activation of HRI by different stresses in reticulocytes. (A) Activation of HRI by osmotic stress and heat shock. Mouse reticulocytes were subjected to osmotic stress (Os) and heat shock (hs) for 30 min and then labeled with [35S]methionine for an additional 30 min. The extents of HRI activation and eIF2 phosphorylation and the incorporation of [35S]methionine into globins were detected as described in the legend to Fig. 1. C, control. (B) Effects of cytoplasmic stresses on HRI activation. Mouse reticulocytes were subjected to stresses as described in Materials and Methods. The extents of HRI activation and eIF2 phosphorylation were examined by Western blot analysis. The amounts of eIF2P under various stresses were quantitated by densitometry scans of Western blots from three separate experiments and are shown here. As, arsenite; AA, amino acid starvation; S, serum starvation. (C) eIF2 kinase activity of HRI. The activity of HRI in either stressed or nonstressed reticulocytes, as indicated, was assayed by IP-coupled protein kinase assays as described in Materials and Methods and the legend to Fig. 1C. The incorporation of 32P into eIF2 was detected by autoradiography. NI, nonimmune serum control. (D) Effect of alkaline phosphatase (AP) on the electrophoretic mobility of HRI. Cell lysates from either stressed or nonstressed reticulocytes were treated with or without 2 U of calf intestine alkaline phosphatase at 37°C for 30 min. The phosphorylation status of HRI was analyzed by SDS-PAGE and Western blot analysis. (E) Autophosphorylation is required for the slower electrophoretic mobility of the HRI activated by arsenite treatment. Wild-type (Wt) HRI and its inactive mutant K196R HRI were expressed in CHO-TRX cells by induction with tetracycline (Tet) overnight as described in Materials and Methods. Cells were treated with arsenite for 1 h. The expression and activation of HRI and its mutant in CHO cells were examined as described above.

View larger version (39K): [in this window] [in a new window]   FIG. 3.   Activation of HRI by various cytoplasmic stresses in fetal liver erythroid progenitors. (A) Effect of arsenite. Fetal liver cells from both wild-type and HRI/ mice were isolated as described in Materials and Methods. Cells were treated with different concentrations of arsenite (As) for 1 h. C, control. (B) Effect of osmotic stress (Os), heat shock (hs), and Tg. Fetal liver cells were treated with different cytoplasmic stresses: osmotic stress by NaCl (0.5 M, 1 h), heat shock (42°C, 30 min), and ER stress by Tg (1 µM, 1 h). (C) Effect of amino acid starvation and serum starvation. Starvation of cells was performed by incubation in either methionine (Met)-free or serum-free medium for 1 h. (D) Effects of arsenite treatment on protein synthesis in wild-type and HRI/ fetal liver cells. Fetal liver cells were subjected to arsenite treatment for 90 min. [35S]methionine was added at time zero. Cells were harvested at different time periods as indicated. The total cellular proteins were separated by SDS-PAGE. The incorporation of [35S]methionine into globins was detected by autoradiography and was quantitated by scintillation counting of nitrocellulose strips containing globin chains.

HRI is the only eIF2 kinase activated by arsenite in erythroid cells. As described above, HRI can be activated by cytoplasmic stresses such as arsenite treatment, heat shock, and osmotic stress. It remains to be determined how important HRI is in these stress responses. Is HRI the only eIF2 kinase responsible for these stresses? Other eIF2 kinase, especially PKR, are known to be present in reticulocytes and may also be involved in these stress responses. PKR^/ reticulocytes and HRI^/ reticulocytes and fetal liver cells were used to address this question. Arsenite treatment with either different concentrations (Fig. 3A and 4A) or different time periods (data not shown) failed to increase eIF2 phosphorylation in HRI^/ cells. Furthermore, arsenite treatment resulted in the inhibition of protein synthesis in HRI^+/+, but not HRI^/, fetal liver cells (Fig. 3D). This result demonstrates that HRI is the only eIF2 kinase activated in erythroid cells upon arsenite treatment. In contrast, increased eIF2 phosphorylation was still observed in HRI^/ reticulocytes and fetal liver cells under other stresses, such as heat shock and osmotic stress (Fig. 3B and 4A). Thus, even though HRI is activated by these stresses, it is not the only eIF2 kinase responsible for increased eIF2 phosphorylation under stress. Other eIF2 kinases may be activated and are also involved in heat shock and osmotic stress responses. However, HRI is the predominant eIF2 kinase activated in heat shock, since the extent of eIF2 phosphorylation was significantly reduced in HRI^/ cells compared to in wild-type cells. In osmotic stress, other eIF2 kinase members or phosphatases contribute more significantly, since no significant difference in eIF2 phosphorylation was observed between wild-type and HRI^/ cells.

View larger version (33K): [in this window] [in a new window]   FIG. 4.   Cytoplasmic stresses of wild-type, HRI/, and PKR/ reticulocytes. (A) eIF2 phosphorylation of wild-type, HRI/, and PKR/ and reticulocytes upon different stresses. Reticulocytes from wild-type, HRI/, or PKR/ mice were subjected to different stress conditions as described in the legend to Fig. 2A. As, arsenite; Os, osmotic stress; hs, heat shock; C, control. (B) eIF2 kinase activity of PKR. PKR from either stressed or nonstressed reticulocytes was immunoprecipitated with anti-PKR antibody. The kinase activity of PKR was determined by in vitro kinase assays as described in Materials and Methods. The incorporation of 32P into eIF2 was detected by autoradiography. NI, nonimmune serum control.

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Role of ROS in stress-induced activation of HRI. Stress-activated signaling cascades may be mediated by an altered redox potential in the cell during oxidative stress. The key contributors to altered redox potential are reactive oxygen species (ROS), which are formed in most cases by extracellular stress conditions. Arsenite is thought to be an oxidative stress inducer and can cause increases of intracellular ROS (10). Arsenite activation of HRI was found to occur only in intact reticulocytes and not in reticulocyte lysates (Fig. 1C). Thus, arsenite does not act directly on HRI.

View larger version (64K): [in this window] [in a new window]   FIG. 5.   Role of ROS in HRI activation. Mouse reticulocytes were pretreated with different concentrations of the reducing reagent NAC as indicated for 30 min before being subjected to different stresses: no stress treatment (C), arsenite treatment (200 µM) for 1 h (As), heat shock at 42°C for 30 min, and osmotic stress with 0.5 M NaCl for 1 h. The activation of HRI and eIF2aP was examined.

Role of heat shock protein chaperones in HRI activation under stress conditions. Both hsp90 and hsc70 have been shown to interact and regulate HRI activity in vitro (41, 42). We have examined the possible involvement of these two chaperones in the activation of HRI in intact reticulocytes upon stress. Reticulocytes were pretreated with GA, a specific inhibitor of hsp90 (29), or with CIA, an hsc70 antagonist (42), for 30 min prior to arsenite treatment. Our results demonstrate that the activation of HRI by arsenite is prevented by GA or CIA in a dose-dependent manner with a concomitant decrease of eIF2 phosphorylation (Fig. 6). CIA or GA alone had no significant effect on HRI activation or eIF2P. Similar results were observed under heat shock and osmotic stress. Thus, these results strongly suggest that hsp90 and hsc70 are required for the activation of HRI by cytoplasmic stresses in intact reticulocytes.

View larger version (47K): [in this window] [in a new window]   FIG. 6.   Role of chaperones in activation of HRI by arsenite. Reticulocytes were pretreated with different concentrations of GA (4, 8, 20, and 40 µg/ml) (B) and CIA (5, 10, 20, and 40 mM) (A) for 30 min and then subjected to a 60-min incubation with (As) or without (C) arsenite treatment at 200 µM. The activation of HRI and eIF2P was examined.

	DISCUSSION

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It has been well established that HRI is activated in heme deficiency of reticulocytes (reviewed in reference 8). Our recent study of HRI^/ mice demonstrates the physiological function of HRI in balancing globin synthesis with the availability of heme (14). However, it remains to be determined whether HRI also plays a role in regulating protein synthesis under other stress conditions. Here, we report a novel function of HRI in response to cytoplasmic stresses other than heme deficiency in both reticulocytes and immature nucleated erythroid cells. By using HRI^/ reticulocytes and fetal liver erythroid progenitor cells, we demonstrate that HRI is the only eIF2 kinase activated by arsenite in erythroid cells and is responsible for the increased eIF2 phosphorylation caused by arsenite treatment in these cells.

It has been speculated for some time about which eIF2 kinase family member is responsible for the increased eIF2 phosphorylation and the subsequent inhibition of protein synthesis caused by cytoplasmic stresses such as arsenite treatment, heat shock, and osmotic stress (4). Among the four known eIF2 kinases, PERK is not activated by either arsenite or heat shock (17). GCN2 was originally discovered in yeast and is activated during amino acid starvation (20). It was later also found in Drosophila, mouse, and human (3, 40). It has been reported that GCN2 can also be activated by glucose starvation (43) or serum starvation (3). To date, the contribution of GCN2 to arsenite treatment and other cytoplasmic stresses is still unknown. Our present study indicates that GCN2 is not likely to be activated by arsenite. As shown in Fig. 3, both wild-type and HRI^/ fetal liver cells responded to amino acid and serum starvation, indicating the presence of functional GCN2. However, HRI^/ fetal liver cells failed to respond to arsenite despite the response to amino acid and serum starvation.

In nonerythroid cells, PKR is likely the eIF2 kinase activated by arsenite, since little or no HRI is expressed in nonerythroid cells (13). It has been reported that PKR is activated by arsenite in murine interleukin-3-dependent NFS/N1.H7 cells (21) and NIH 3T3 and MEF cells (30). In addition PKR-null MEF cells are more resistant to arsenite-induced apoptosis (30). Recently, a cellular protein activator of PKR was cloned from human (PACT) (31) and mouse (RAX) (21). PACT/RAX is a dsRNA-binding protein, forms a heterodimer with PKR through its dsRNA-binding motifs, and activates PKR in vitro in the absence of dsRNA (21, 31). Arsenite treatment induces rapid phosphorylation of PACT/RAX and the association with PKR (30). However, we found here that PKR is not essential for arsenite-induced eIF2 phosphorylation in reticulocytes (Fig. 4A) and is not activated by arsenite in mouse erythroid cells as examined by IP-coupled protein kinase assays (Fig. 4B). One reason for this is that the activator of PKR, PACT/RAX, may be lacking or unable to be phosphorylated in erythroid cells upon stress. Therefore, PKR cannot be activated by arsenite in erythroid cells.

Since both PKR and HRI can be activated by various stresses, the involvement of these two eIF2 kinases seems to depend on the tissue-specific expression of these kinases. In erythroid cells, HRI is the most predominant eIF2 kinase and is responsible for the phosphorylation of eIF2 by arsenite and most other cytoplasmic stresses. In nonerythroid cells like NIH 3T3 and MEF cells, it is likely that PKR is activated by these stresses, since there is little to no HRI in these cells. Our results also indicate that ER stress and amino acid starvation are unique to PERK and GCN2, respectively. These stresses do not activate HRI (Fig. 1, 2, and 3). Similarly, it has been shown recently that PKR is dispensable for ER stress and amino acid starvation (24). The possible role of GCN2 in cytoplasmic stress remains to be investigated when GCN2^/ cells are available.

In the case of high-salt osmotic stress, the response is different from that to either arsenite or heat shock. There is no significant reduction of eIF2P in either HRI^/ or PKR^/ reticulocytes. It is possible that GCN2 or PERK may be another eIF2 kinase responding to osmotic stress.

Unlike the regulation of HRI by heme, which binds directly to HRI and inhibits its activity, activation of HRI by arsenite treatment or heat shock is independent of heme. This conclusion is supported by two different experimental approaches. One of them is heme reversibility. Activation of HRI in heme deficiency in intact reticulocytes can be prevented by adding heme to the cells (14) (Fig. 1D), while arsenite activation of HRI cannot be suppressed by addition of hemin (Fig. 1D). This result indicates that the mechanisms of HRI activation by heme deficiency and arsenite treatment differ. The other supporting evidence is provided by the requirement of the intact living cells for the activation of HRI by arsenite and heat shock but not by heme deficiency. Once reticulocytes were lysed, HRI could not be activated by treatment with arsenite or heat shock (Fig. 1C). Thus, activation of HRI by arsenite is indirect and requires other components of the living cell. Furthermore, the difference in mechanisms of HRI activation by arsenite and heme deficiency is also indicated by the different phosphorylation statuses of HRI under these conditions. The upshift of HRI in SDS-PAGE caused by arsenite treatment, heat shock, and osmotic stress is higher than that caused by heme deficiency (data not shown).

A model summarizing stress activation of eIF2 kinases in erythroid cells is shown in Fig. 7. Under stress conditions, HRI serves a major role in regulating protein synthesis in erythroid cells. It can be activated by several different stresses other than heme deficiency. Activation of HRI by heme deficiency occurs intracellularly, while activation by other stresses requires the presence of the intact cells. Arsenite-induced eIF2 phosphorylation is mediated exclusively through the activation of HRI, while increased eIF2 phosphorylation under osmotic stress or heat shock is mediated through activation of both HRI and other eIF2 kinases or through the inhibition of phosphatases. HRI contributes more significantly under heat shock than under osmotic stress. The mechanism of the activation of HRI by arsenite is different from that of activation by heat shock or osmotic shock. ROS are involved in the signaling of arsenite activation of HRI only. Furthermore, HRI activated by arsenite is hyperphosphorylated compared to HRI activated by heat shock or osmotic shock. HRI is not activated by nutrient starvation or by ER stress in either reticulocytes or fetal liver erythroid progenitor cells.

View larger version (33K): [in this window] [in a new window]   FIG. 7.   Model of the role of HRI in the stress response of erythroid cells. HRI is a major stress-activated eIF2 kinase in erythroid cells and can be activated by several different stresses in addition to heme deficiency. HRI is the only eIF2 kinase activated by arsenite. It is also the main eIF2 kinase activated by heat shock. The activation of HRI contributes to the regulation of cell proliferation, survival, and/or differentiation under stress challenges. AA, amino acid.

Both arsenite treatment and heat shock are known to activate the mitogen-activated protein (MAP) kinases, including ERKs, p38, and JNKs (32). The MAP kinase pathway is also important for activation of many signaling targets, which lead to modification of transcriptional factors and translational regulators (27). We have found that ERKs, JNKs, and p38 were expressed and were activated by arsenite in fetal liver cells (data not shown). However, we found that the ERK inhibitor PD98059 (27) and the p38 inhibitor SB203580 (36) had no significant effect on HRI activation and eIF2 phosphorylation in fetal liver cells or reticulocytes (data not shown). Similarly, we found that staurosporine, which is known to inhibit all MAP kinases but not eIF2 kinases (26), had no significant effect on HRI activation either (data not shown). Thus, it appears that the activation of HRI by stresses does not require the activation of MAP kinases.

Stress-activated signaling cascades can be affected by altered redox potential in the cell. ROS are a key contributor to altered redox potential (reviewed in reference 35). Arsenite can cause the formation of ROS and is thus thought to be an oxidative stress inducer (10). ROS are considered to be the primary intracellular change that serves as an important cellular component linking external stimuli with signal transduction. ROS can modulate signaling molecules, including both kinases and transcription factors. One of the most well-known targets of ROS is the stress-activated kinase (1). In this study, we also found that HRI activation by arsenite depends on ROS formation in the cell. ROS can modulate signaling molecules through two different means (37). One is to oxidize the cysteine of the target proteins directly and alter conformation and activity. The other is through cysteine-rich, redox-sensitive proteins such as thioredoxin and glutathione. It remains to be determined how ROS regulate the activity of HRI. However, it seems likely that glutathione acts as a mediator. The translation rate in rabbit reticulocyte lysates can be inhibited by GSSG (the oxidized form of glutathione) through the activation of HRI (11, 22).

The chaperone hsp90 is known to be a kinase chaperone, as it binds to various kinases and regulates their activities (5). Its role in the folding and maturation of newly synthesized HRI has been demonstrated in rabbit reticulocyte lysates (41). We show here that hsp90 is also essential for HRI activation under stress conditions. The mechanism by which hsp90 works in this process is still not clear. Both the nonactivated form and stress-activated hyperphosphorylated forms of HRI can be coimmunoprecipitated with hsp90 (data not shown).

The role of hsc70 in HRI regulation seems to be more complicated. A dual role of hsc70 in HRI regulation has been suggested by Uma et al. (42). Their in vitro study using rabbit reticulocyte lysates indicates that hsc70 is required for the folding and transformation of HRI into an active kinase and is subsequently required to negatively attenuate the activation of transformed HRI. Disruption of Hsc70 function by CIA in heme-deficient reticulocyte lysates resulted in the hyperactivation of HRI. However, this is not the case in intact reticulocytes. Treatment of reticulocytes with CIA alone did not cause the activation of HRI. Under cytoplasmic stress induced by arsenite, heat shock, and osmotic stress, CIA inhibits the stress-induced activation of HRI. Thus, in living cells, hsc70 is essential for HRI activation by stresses.

Contamination of drinking water with arsenic compounds poses an important health hazard. Long-term arsenic exposure can lead to many kinds of cancers, such as lung, bladder, and skin cancers, as well as to defects in erythropoiesis (28, 39). We have shown that HRI is involved in the regulation of erythroid cell proliferation (12). Our recent work on HRI knockout mice also indicates an essential role of HRI in stress-induced erythropoiesis (14). The unique role of HRI in the arsenite response and the availability of the HRI knockout mice generated in our laboratory provide us important tools to elucidate the molecular mechanisms of how arsenite affects erythropoiesis.

In summary, we have discovered a new function of HRI in erythroid cells under cytoplasmic stress responses other than heme deficiency. We also demonstrated the essential role of HRI in arsenite-induced eIF2 phosphorylation and inhibition of protein synthesis in erythroid cells.