INTRODUCTION

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The involvement of calcium in cell death has been widely reviewed over the last several decades. A prevalent assumption is that either a low or high intracellular calcium concentration ([Ca²⁺]_i) can cause cell death because of the critical role played by calcium in many physiological processes. However, it has also been shown that calcium can promote cell survival in a number of systems. For example, a modest elevation of [Ca²⁺]_i above normal resting levels (100 to 150 nM) can promote cell survival in developing neurons (20).

The proapoptotic effects of calcium can vary across cell types and may be mediated by different mechanisms. For example, calcium-dependent induction of Nur77 gene expression is essential for T-cell receptor-induced death in thymocytes (92), whereas calcium-induced activation of calcineurin has been implicated in calcium-induced cell death in other cell types (88). The mechanisms underlying these two types of cell death are relatively clear. Both Nur77 and calcineurin trigger mitochondrial release of cytochrome c and subsequent cell death. The former occurs via direct translocation onto mitochondria (47), and the latter occurs via dephosphorylation of Bad to initiate mitochondrial response (88).

On the other hand, very little is known about calcium-mediated resistance to cell death. Calcineurin has also been suggested to be involved in calcium-mediated cell protection (52). Furthermore, p38 mitogen-activated protein kinase-dependent activation of MEF2C activation may be involved in the calcium-dependent survival of neurons (53, 63). The mechanism by which calcium prevents cell death under these conditions is unknown.

It has been well accepted that the principal role of the lysosome is one of cellular housekeeping. Lysosomes remove damaged macromolecules from the cellular environment and convert them into reusable products. However, recent works demonstrated that lysosomal exocytosis occurs in many cell types, suggesting that this organelle may not be just a final station of the endocytic pathway but may have other functions (2, 25, 75, 76, 85). At one time, lysosomes were proposed to be "suicide bags," or prepackaged destruction awaiting a programmed instruction to strike (14). The suicide bag theory has not endured because it lacks supporting evidence (50). However, the possibility that lysosomes play a role in cell death cannot be excluded, since over the past 40 years, many groups of investigators have observed alterations in lysosomes during cell death (14, 51, 60).

Leakage of lysosomes has been observed morphologically in several models of cell death, including death caused by oxidative stress and by serum deprivation (7, 65). Autophagy was associated with death in several different cell types, and inhibition of autophagy prevented apoptosis induced by withdrawal of nerve growth factor (36, 62, 91). The role of the lysosome in cell death was further supported by the observation that various lysosomotropic agents, such as ammonium chloride and chloroquine, can prevent certain types of cell death, such as that induced by tumor necrosis factor alpha (TNF) (43, 49, 58). The lysosomal protease cathepsin D is involved in a number of types of apoptosis, including that induced by gamma interferon, Fas, and TNF (15, 28, 34, 67, 73). Although the available data are somewhat limited, there is a growing body of evidence suggesting that lysosomes actively participate in cell death.

TNF is a proinflammatory cytokine that acts as a mediator of host defense as well as inflammatory diseases (3, 4). TNF was originally identified and purified as a factor that leads to rapid hemorrhagic necrosis of an established tumor (9, 64). It is now known that TNF can induce cell death in numerous cell types, and it is widely used to study the mechanisms of cell death. The morphological characteristics of cells differ markedly among various TNF-treated cell lines. For example, TNF induces an apoptotic phenotype (chromatin condensation and cell shrinkage in a nearly or wholly intact plasma membrane) in MCF-7, KYM, and PC60 tumor cell lines (19), whereas L929 and WEHI 164 clone 13 cells respond to TNF with characteristics of necrosis (5, 19).

It is known that death signaling in both apoptotic and necrotic cell death is initiated by clustering of the TNF receptor-1 and recruitment of the death domain-containing adapter protein TRADD (1, 19, 33). The subsequent apoptosis of TNF-treated cells is mediated by activation of caspases and release of cytochrome c from the mitochondria (48, 82, 83), while the necrotic cell death of L929 cells is independent of caspases (86).

In an effort to identify genes that mediate the cytolytic effect of TNF, we have used a random gene disruption approach to generate a series of L929 mutants that are resistant to TNF-induced cell death. The gene disrupted in one of the TNF-resistant lines was identified as PMCA4, a ubiquitously expressed protein that couples the extrusion of calcium across the plasma membrane with ATP hydrolysis (22, 27). To date, four PMCAs (PMCA1 to -4) have been identified. PMCA1 and PMCA4 are expressed in almost all tissues, whereas PMCA2 is expressed primarily in brain and heart and PMCA3 expression is essentially confined to brain and skeletal muscle. PMCAs were believed to be Ca²⁺ pumps that maintain basal levels of intracellular calcium (22, 27). Here we show that resistance to cell death in PMCA4-deficient L929 fibroblasts is mediated by a modest elevation in [Ca²⁺]_i. We found that an increase in lysosome volume is a common feature of cells that undergo either apoptotic or necrotic cell death. We further show that increased levels of [Ca²⁺]_i in PMCA4-deficient cells enhanced lysosomal exocytosis. This increase in [Ca²⁺]_i levels accounts, at least in part, for the diminished lysosomal response in PMCA4-deficient cells. The impaired lysosomal response leads, in turn, to TNF resistance.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Cell culture. All cell lines were obtained from the American Type Culture Collection (Rockville, Md.) and cultured under the recommended conditions. TNF sensitivity was examined in L929 murine fibrosarcoma cells, which exhibited a spontaneous survival rate of less than 1 in 10⁶ after 48 h of exposure to TNF at 100 ng/ml. Stable TNF-resistant cell lines derived from L929 cells were established either by retroviral infection or by plasmid transfection with the GenePorter transfection reagent (GTS Inc., San Diego, Calif.). Stably transfected TNF-resistant clones were selected in normal growth medium with the addition of either G-418 (1 mg/ml) or blasticidin S (10 µg/ml) (Invitrogen, Carlsbad, Calif.).

Retroviral vector construction and subcloning. The pDisrup retroviral vector was constructed based on the Moloney murine leukemia virus retroviral vector pLNCX backbone (56). This vector encodes a neo gene that is driven by the cytomegalovirus (CMV) promoter, but lacks a poly(A) addition signal sequence (89). Standard recombinant DNA techniques were used in vector construction and subcloning. The splicing donor and acceptor sites were designed based on the human adenovirus type 2 major late mRNA intron sequence. Incorporation of the splicing donor sequence downstream from the neo coding sequence permitted the generation of a neo mRNA fused with the downstream exon of a disrupted gene if viral insertion occurred within an intron. If the virus inserted within an exon, the expressed neo mRNA should represent a direct fusion with endogenous exon sequences at the insertion site. neo mRNA cannot be expressed if the insertion occurs in a nongene region. Further details of the retroviral plasmid are available upon request. The PMCA4 cDNA was obtained from Ernst Carafoli and subcloned into the pcDNA6 expression vector, which carries blasticidin resistance (Invitrogen, Carlsbad, Calif.).

Retrovirus production and cell infection. pDisrup recombinant retroviruses were generated in Phoenix Amphotropic producer cells using the calcium phosphate method of transfection (56). Viruses were produced at 32°C, and virus-containing medium was collected 24 h posttransfection and filtered through a 0.45-µm filter. L929 cells were plated in six-well plates at a density of 5 × 10⁵ cells/well. One round of retroviral infection was performed by replacing medium with 2 ml of pDisrup virus (containing 4 µg of Polybrene per ml), followed by centrifugation of the six-well plates at 2,500 rpm for 30 min at 32°C.

3'-RACE. The portion of the endogenous gene that was fused with the neo gene was amplified by the 3' rapid amplification of cDNA ends (RACE) technique. Total RNA was isolated, and reverse transcription was performed with the primer 5'-CCA GTG AGC AGA GTG ACG AGG ACT CGA GCT CAA GC[T]₁₇-3'. A nested PCR was performed with the resulting reverse transcription product with the following primers: P1/Q1 (5'-ATG GGC TGA CCG CTT CCT-3' and 5'-CCA GTG AGC AGA GTG ACG-3') and P2/Q2 (5'-GAC GAG TTC TTC TGA CTA GCT AG-3' and 5'-GAG GAC TCG AGC TCA AGC-3'). P1 and P2 are located on the neo resistance gene, while Q1 and Q2 are on the anchor sequence of QT. The PCR fragments were subcloned into the TA cloning vector (Invitrogen) and subjected to DNA sequencing.

Western blot analysis. Crude membranes were prepared as described (27) and subjected to sodium dodecyl sulfate-6% polyacrylamide gel electrophoresis, followed by standard Western blotting procedures. The monoclonal antibody 5F10 (Affinity Bioreagents, Inc, Golden, Colo.) was used to detect total PMCA immunoreactivity.

Measurement of intracellular calcium. Cells were loaded with 4 µg of fluo-3 acetoxymethyl (AM) (Molecular Probes, Inc., Eugene, Oreg.) and 10 µg of Fura-Red AM (Molecular Probes, Inc.) per ml in the presence of 4 mM probenecid (Sigma, St. Louis, Mo.) at 37°C for 1 h, according to published protocols (61). Flow cytometry analysis was performed at 488 nm excitation, the fluorescence of fluo-3 was collected at 520 nm, and Fura-Red emission was collected at 640 nm. An in situ calibration assay for calcium measurements in L929 cells was performed as described (37). The fluo-3/Fura-Red ratio versus calcium concentration was plotted and used to calculate [Ca²⁺]_i in each sample as described (37).

Measurement of VAC. Cells in various stages were harvested and then incubated at 37°C for 15 min with 100 ng/ml LysoTracker (Molecular Probes, Inc.) or 5 µg/ml acridine orange (AO) (Sigma, St. Louis, Mo.). Cells were then washed, pelleted, and resuspended in 1 ml of phosphate-buffered saline (PBS) for measurement of fluorescence derived from the aggregated LysoTracker or AO in acidic compartments. The healthy cells (with intact lysosomes) were gated, and the mean value of the fluorescence intensity was used as the relative value of total volume of the acidic compartment (VAC).

Cell viability assay using PI staining plus forward-angle light scattering. The integrity of the plasma membrane was assessed by the ability of cells to exclude propidium iodide (PI) (Sigma, St. Louis, Mo.). Cells were trypsinized, collected by centrifugation, washed once with PBS, and resuspended in PBS containing 1 µg/ml PI. The levels of PI incorporation were quantified by flow cytometry on a FACScan flow cytometer. Cell size was evaluated by forward-angle light scatting. PI-negative cells of normal size were considered live cells.

Measurement of lysosme pH. Double fluorescein- and tetramethylrhodamine-labeled dextran (FTD) (Molecular Probes) was used as a ratiometric indicator to study the pH change of lysosomes. L929 cells were labeled with FTD (3 mg/ml) for 30 min and then changed to FTD-free medium for 60 min. FTD was internalized and selectively localized in lysosomes (21). The ratio of fluorescein emission at 520 nm to rhodamine emission at 580 nm was used to calculate pH. The calibration and calculation of fluorescein emission-to-rhodamine emission ratio were performed as described (23).

Measurement of intracellular pH. 2',7'-bis-(2-carboxyethyl)-5-(and -6)-carboxyfluorescein (BCECF) was used as a ratiometric dye for intracellular pH measurement. Cells were loaded with 2 µg of BCECF AM per ml. Emission fluorescences at 525 and 640 nm were collected. The calibration and calculation of the 525 nm:640 nm emission ratio were performed as previously described (11).

Measurement of endocytosis and exocytosis. The endocytosis of TNF-treated L929 cells was measured as described (57, 87). Briefly, the cells were treated with TNF for 0.5, 1, 2, 4, 6, or 8 h and then cultured with dextran conjugated with tetramethylrhodamine (Molecular Probes) (3 mg/ml). The cells were washed after 30 min, and the uptake of dextran was determined by fluorescence-activated cell sorting (FACS) analysis. Exocytosis was measured by monitoring the release of dextran that was taken up into lysosomes (21). Briefly, cells were labeled with dextran conjugated with tetramethylrhodamine for 30 min. The cells were washed and incubated in dextran-free medium for an additional hour to allow all conjugated dextran to be taken up into lysosomes. The cells were treated with TNF for different periods of time, and the dextran that was retained inside the cells was determined by FACS analysis (21).

-Hexosaminidase activity assay. Confluent monolayers of L929 cells in culture dishes were washed with PBS and incubated with 0.5 ml of Dulbecco's modified Eagle's medium in the absence of phenol red for the indicated times. N-Acetyl--D-glucosaminidase (-hexosaminidase) activity in the culture medium and in the cells was measured as described previously (69).

Transmission electron microscopy. A standard protocol was used to prepare the samples for electron microscopy (45). The experiments were performed by the Core Electron Microscope Facility of the Scripps Research Institute using a Philips CM-100 electron microscope. Specific images were recorded photographically using Kodak SO-163 film.

Electrophoretic mobility shift assay. For the electrophoretic mobility shift assay, nuclear extracts of cells were incubated with a double-stranded, ³²P-labeled oligonucleotide containing an NF-B binding site as a probe as described elsewhere (41)

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

Disruption of PMCA4 gene in L929 cells confers resistance to TNF-induced cell death. We used retrovirus insertion-mediated mutagenesis in L929 fibroblasts coupled with TNF treatment to select TNF-resistant cell lines that were generated by the mutagenesis. As described in Materials and Methods, the retroviral vector was designed so that the neo gene was fused to the sequence of the exon that was at the 3' end of the viral insertion site. The identities of the disrupted genes in various TNF-resistant cell lines were determined by 3'-RACE of the fused neo mRNA.

View larger version (51K): [in this window] [in a new window]   FIG. 1.   PMCA4 is involved in TNF-induced cell death, and [Ca2+]i influences TNF sensitivity in L929 cells. (A) The fused mRNA of neo and an endogenous gene in a TNF-resistant L929 clonal cell line was amplified by 3'-RACE. The junction sequence of the fused cDNA is shown, which reveals that the viral insertion occurred at the 5' end of the PMCA4 gene. Amino acid sequence at the C terminus of neo is shown beneath the mRNA sequence. The sequence introduced by viral vector is shown in lowercase. The number in parentheses shows the beginning of the PMCA4 gene relative to its start codon (+1). (B) PMCA protein is reduced in PMCA4 mutant cells (PMCAmut). An antibody that recognizes all four PMCA isoforms was used in Western blotting analysis on membrane protein samples from PMCAmut and parental wild-type L929 cells (PMCA1 to -4 are each 130 kDa, as indicated by the arrow). A nonspecific band in the Western blot that shown in the lower panel indicates equal protein loading. (C) Total RNA was prepared from PMCAmut and two other clonal cell lines that had viral insertions in other loci. neo mRNA levels were analyzed by Northern blot using a 32P-labeled double-stranded neo probe. A single neo fusion mRNA was detected in PMCAmut cells, as indicated by the triangle. The asterisk indicates transcripts driven by the 5' long terminal repeat, which was detected in all three lines by the neo probe because it contains antisense sequence of neo. Arrows show the positions of 28S and 18S rRNA. (D) Stable cell lines were generated from PMCAmut cells by transfection with either a wild-type PMCA4 expression vector (reconstituted) or the empty vector (vector). All three cell lines, together with parental wild-type L929 cells, were treated with TNF (100 ng/ml), and cell viability was determined by PI exclusion plus cell size at various time points (see Materials and Methods for details). The samples were analyzed by flow cytometry (FACScan flow cytometer [Becton Dickinson]) with CellQuest acquisition and analysis software. (E) Parental L929, PMCAmut, reconstituted, and vector cells were treated with TNF for various periods of time as indicated. Live cells were gated as in panel D, and [Ca2+]i was measured by fluo-3 and Fura-Red fluorescence ratios. Data are expressed as average ± standard error of the mean as determined in triplicate samples.

Impaired dynamic Ca²⁺ regulation in response to TNF in PMCA^mut cells. Since PMCA4 functions in maintaining the basal [Ca²⁺]_i, we next compared [Ca²⁺]_i in these various cell lines. Calcium concentration was measured by flow cytometry using fluo-3/Fura-Red ratios as described (61). Fluo-3 emits fluorescence upon binding to Ca²⁺ and the fluorescence of simultaneously loaded Fura-Red was used to normalize dye loading. Under basal conditions in normal growth medium, [Ca²⁺]_i levels were identical in parental L929 cells, PMCA^mut cells, PMCA4-reconstituted cells, and vector-transfected cells (Fig. 1E). In contrast, when the cells were treated with TNF, [Ca²⁺]_i increased by 35 nM in parental and in PMCA4-reconstituted cells, whereas [Ca²⁺]_i increased by 100 nM in PMCA^mut cells and vector-transfected cells. The higher [Ca²⁺]_i in PMCA4-deficient cells (Fig. 1E) was correlated with a higher survival rate of these cells after TNF treatment (Fig. 1D), suggesting that the elevation in [Ca²⁺]_i prevents TNF-induced cell death.

Increased [Ca²⁺]_i protects against cell death in L929 cells. It has been well established that modest elevations in [Ca²⁺]_i can prevent cell death in developing neurons (20). The protective effect of increased [Ca²⁺]_i has also been observed in other systems (52, 70). The data presented in Fig. 1D and 1E suggested that the elevated [Ca²⁺]_i also protects L929 cells against TNF-induced death. To further establish whether the TNF resistance observed in the PMCA^mut line resulted from increases in [Ca²⁺]_i, we treated parental L929 cells with TNF in the presence of various concentrations of either LaCl₃, an inhibitor of PMCA (31), or A23187, a calcium ionophore. Both LaCl₃ and A23187 treatment elevated [Ca²⁺]_i and enhanced survival in TNF-treated L929 cells (Fig. 2A and 2B). Optimal protection against TNF-induced cell death was observed at a [Ca²⁺]_i of 200 nM (100 nM above the basal level) (Fig. 2A and 2B). Similar [Ca²⁺]_i levels were observed in PMCA^mut cells treated with TNF (Fig. 1E).

View larger version (30K): [in this window] [in a new window]   FIG. 2.   Increase in [Ca2+]i induces resistance to cell death in L929 cells. (A) Parental L929 cells were treated with TNF in the presence or absence of various concentrations of LaCl3, an inhibitor of PMCAs, for 24 h as indicated. [Ca2+]i and cell viability were determined. (B) Parental L929 cells were treated with TNF in the presence or absence of various concentrations of the calcium ionophore A23187 for 24 h as indicated. [Ca2+]i and cell viability were determined. (C) Parental and PMCAmut cells were treated with staurosporine, etoposide, or lonidamine in the presence or absence of A23187 for 36 h. Cell viability was determined.

View larger version (44K): [in this window] [in a new window]   FIG. 3.   PMCA4 deficiency did not influence TNF-induced NF-B activation, and its effect on cell death did not require new protein synthesis. (A) Parental and PMCAmut cells were treated with TNF for different periods of time as indicated. NF-B activation was measured by electrophoretic mobility shift assay. (B) Parental and PMCAmut cells were treated with TNF or TNF and cycloheximide (CHX, 10 µg/ml) for different periods of time. Cell viability was determined.

Disruption of calcium regulation may affect lysosomal function in TNF-treated cells. The effect of the PMCA inhibitor LaCl₃ and the calcium ionophore A23187 on TNF-induced cell death was compared in cells expressing normal levels of PMCA4 (parental and reconstituted cells) and cells deficient in PMCA4 (PMCA^mut and vector-transfected PMCA^mut) (Fig. 4A). When the samples were treated with TNF alone, parental and reconstituted cells had an average survival rate of less than 20%, while PMCA^mut and vector-transfected PMCA^mut cells had a survival rate of 45% (Fig. 4A). There is a more than twofold difference in survival rate between PMCA4-normal and PMCA-deficient cells. However, when the cells were cotreated with LaCl₃ or A23187 and TNF, the survival rates of PMCA4-normal and -deficient lines became comparable and increased to 50% (Fig. 4A). The similar survival rate of the TNF-treated cells of the four lines in the presence of LaCl₃ or A23187 further supported the conclusion that PMCA4 deficiency and LaCl₃ and A23187 affected the same event, [Ca²⁺]_i.

View larger version (49K): [in this window] [in a new window]   FIG. 4.   Increases in [Ca2+]i and inhibition of lysosomal activity can abolish the differences in TNF sensitivity between parental and PMCAmut cells. Parental L929, PMCAmut, reconstituted, and vector cells were treated with TNF in the presence or absence of various inhibitors, as indicated, and cell viability was determined 24 h later. (A) The four cell lines were cotreated with TNF and either the calcium ionophore A23187 (1 µM) or the PMCA inhibitor LaCl3 (20 µM), and the resulting survival rates are shown. (B) The four cell lines were cotreated with TNF and either the free-radical scavenger butylated hydroxyanisole (BHA, 50 µM) or complex II inhibitor thenoyltrifluoroacetone (TTFA, 250 µM), and the resulting survival rates are shown. (C) The four cell lines were cotreated with TNF and either a phospholipase A2 inhibitor, aristolochic acid (ArA, 50 µM), or a phospholipase C inhibitor (D-609, 50 µg/ml), and the resulting survival rates are shown. (D) The four cell lines were cotreated with TNF and either chloroquine (25 µM) or pepstatin A (50 µM), and the resulting survival rates are shown.

TNF increases lysosome volume, and disruption of PMCA4 gene impairs this effect. We next asked whether lysosomes are involved in TNF resistance that is associated with disruption of the PMCA4 gene in L929 cells. LysoTracker is an acidotropic probe that can be used to stain lysosomes and other types of acidic compartments. AO is a weak basic amine that selectively accumulates in cellular compartments of low internal pH and is also widely used for staining lysosomes (44, 65). Parental L929 and PMCA^mut cells were treated with TNF, stained with LysoTracker and AO, and analyzed microscopically.

View larger version (46K): [in this window] [in a new window]   FIG. 5.   TNF treatment increases VAC, and this effect is inhibited in PMCA4-deficient cells. The TNF-induced increase in VAC correlates with cell death. (A) Parental L929 and PMCAmut cells were untreated () or treated with TNF (+) for 8 h. Cells were then stained with LysoTracker and AO and analyzed by fluorescence microscopy. (B) Electron microscopy was used to analyze ultrastructural features of parental and PMCAmut cells after 8 h of TNF treatment. Arrows indicate enlarged lysosomes, which should be the major contributors to increased staining of acidic compartments in TNF-treated parental cells observed in panel A. Bars indicate the magnification scale. (C) Lysosome changes in parental L929 cells treated with TNF for different periods of time. The cells were stained with AO. (D) Cells were treated with TNF for various periods of time as indicated. The value of VAC was measured by flow cytometry using LysoTracker or AO staining as described in the text. LysoTracker and AO intensity in live cells was plotted to show the relative change in VAC. Parental and reconstituted cells had a significantly larger increase in VAC in response to TNF than PMCAmut and vector control cells. (E) The survival rate of the cells shown in panel A is shown. The level of the increase in VAC (panel A) correlated with the rate of cell death (panel B).

Calcium-enhanced lysosomal exocytosis is responsible, at least in part, for lack of TNF-induced increase in VAC in PMCA^mut cells. Calcium is known to stimulate lysosomal exocytosis in a number of different cell types, including fibroblasts (2, 68, 69). Therefore, it is possible that increased [Ca²⁺]_i in TNF-stimulated PMCA^mut cells promotes lysosome exocytosis. This could impede the increase in lysosomal mass observed in parental cells. To test this hypothesis, we measured lysosomal exocytosis in TNF-treated parental and PMCA^mut cells. Dextran can be imported into the cell by endocytosis and retained within lysosomes (21). Thus, pulse-chase experiments can be used to measure the rate of dextran exocytosis.

View larger version (19K): [in this window] [in a new window]   FIG. 6.   Lysosomal exocytosis is more rapid in PMCAmut cells than in parental cells. (A) Cells were incubated with tetramethylrhodamine-coupled dextran (2 mg/ml) for 1 h to load lysosomes with dextran. The cells were then washed and incubated in dextran-free medium for another hour and then treated with TNF. Dextran retention was measured by flow cytometry at the indicated time points after TNF treatment. (B) Parental and PMCAmut cells were switched to fresh medium and untreated or treated with TNF for 2 h as indicated. Activity of the lysosomal enzyme -hexosaminidase was measured in the medium and in the cells. The amount of enzyme in the medium is expressed as a percentage of the total enzyme level found in the cells. (C) Cells were treated with A23187 and TNF changes in VAC are shown.

Lysosomal response and cell death. To examine the requirement for the lysosomal response in TNF-induced cell death, we thought to test whether inhibition or enhancement of the increase in VAC influenced TNF-induced cell death. TNF-induced increase in VAC in parental L929 cells was inhibited by A23187 (Fig. 7A, left panel). The levels of the VAC were correlated with rate of cell death (Fig. 7A, right panel). PMCA^mut cells already had much less TNF-induced increase in VAC, and A23187 did not have much effect on VAC and cell death induced by TNF in PMCA^mut cells (Fig. 7A). These data support the idea that the inhibition of the lysosomal response diminished TNF-induced cell death.

View larger version (37K): [in this window] [in a new window]   FIG. 7.   Inhibition of TNF-induced increase in VAC in parental L929 cells increases survival rate, and enhancing VAC increases TNF-induced cell death in PMCA4mut cells. (A) Cells as indicated were treated with TNF in the presence or absence of A23187 for different periods of time. VAC (left panel) and cell viability (right panel) were measured. A23187 inhibited TNF-induced increase in VAC and cell death in parental cells. (B) Parental L929 (top panels) and PMCAmut (lower panels) cells were treated with TNF in the presence or absence of different inhibitors of exocytosis as indicated: nocodazole (Noc), 10 µM; jasplakinolide (Jas), 0.3 µM; and MDL-12330A (MDL), 50 µM. VAC (left panels) and cell viability (right panels) were measured at different time points after TNF treatment. The exocytosis inhibitors did not influence the TNF-induced increase in VAC and cell death in parental cells. In contrast, inhibition of exocytosis enhanced the TNF-induced increase in VAC and cell death in PMCAmut cells. (C) Parental and PMCAmut cells were treated with either 0.3 mM sucrose (Suc) or 0.3 mM glucose (Glu) as indicated. The formation of sucrosomes induced by 0.3 mM sucrose was detected by AO staining, as shown in the left panel. The survival rate of these cells in response to TNF treatment is shown in the right panel. Sucrosomes enhanced TNF-induced cell killing in PMCAmut cells.

Cell death is generally associated with an increase in VAC. We next examined lysosomes in several different cell types that were exposed to various death stimuli. L929 and MCF-7 cells were treated with TNF; PC12 cells were serum deprived; J774 cells were treated with oxidized LDL; Jurkat cells were treated with a Fas antibody; and U937 cells were treated with ceramide. As shown in Fig. 8A, increased LysoTracker staining was observed in each of the samples exposed to a death stimulus. The relative VAC is shown in Fig. 8B and 8C. VAC changes varied among the different cell types. The increase in VAC did not correlate with the type of cell death (either apoptotic or necrotic), but rather seemed to be related to whether the cells were adherent or nonadherent. Suspended cells (Jurkat and U937 cells) exhibited much fewer lysosomal changes than adherent cells (L929, PC12, J774, and MCF-7 cells), which is probably due to suspended cells' having less cytosole than adherent cells. Nevertheless, an increase in VAC is a common event across many, if not all, cell death processes, including apoptosis and necrosis.

View larger version (70K): [in this window] [in a new window]   FIG. 8.   Lysosomal response in different models of cell death. Several different cell culture systems were used to evaluate the change in VAC during the cell death process. L929 and MCF-7 cells were treated with TNF (100 ng/ml) for 8 h; PC12 cells were serum deprived for 6 days; J774 cells were treated with oxidized LDL (100 µg/ml) for 3 days; Jurkat cells were treated with Fas antibody (0.5 µg/ml) for 3 h; and U937 cells were treated with ceramide (100 µM) for 4 h. (A) Lysosomes were stained with LysoTracker and morphologically analyzed by fluorescence microscopy. (B and C) VAC was measured using LysoTracker staining (B) or AO staining (C). The relative LysoTracker or AO intensity is expressed as percent change from the control and was calculated by dividing the arbitrary fluorescence intensity unit number by that of corresponding untreated samples.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

Using a random mutagenesis approach, we found that disruption of the PMCA4 gene produced a resistance to TNF-induced cell death in L929 cells (Fig. 1). We further demonstrated that a modest elevation in [Ca²⁺]_i underlies the resistance to TNF-induced cell death in PMCA^mut cells (Fig. 1 and 2). The calcium-mediated resistance to cell death appeared to be related to lysosome function (Fig. 4). Increased lysosomal volume is a general characteristic of various cell death processes (Fig. 5 and 8). Calcium-promoted lysosomal exocytosis (Fig. 6 and 7) accounted, at least in part, for the inhibition of TNF-induced increase of lysosome volume observed in PMCA^mut, which in turn diminished TNF-induced cell death.

Elevation in [Ca²⁺]_i has long been known to induce apoptotic or necrotic cell death. Multiple mechanisms can mediate this prodeath effect of calcium, which has been studied intensively (59). However, while an antideath effect of calcium has been reported in a number of different cell systems (20, 52, 70), this effect of calcium has largely been neglected (59). Here we provide genetic evidence to support the hypothesis that calcium does indeed have antideath effects. The prodeath effects of calcium on cell death vary considerably in different cell types. The antideath effect of calcium may be similarly dependent on cell type, as calcium promotes lysosomal exocytosis in many, but not all, types of cells (2). It has been reported that Ca²⁺ concentrations between 1 and 5 µM are optimal for exocytosis of lysosomes in permeabilized cells (69). The exocytosis of lysosomal content was detected in our system (intact cells) when the intracellular Ca²⁺ concentration was 200 nM. It is possible that the local concentration of Ca²⁺ in certain cell compartments of our cell systems had reached the micromolar range or that 200 nM Ca²⁺ is sufficient to promote lysosomal exocytosis in live cells. Our data did not exclude the possibility that the inhibition of TNF-induced increase in VAC by calcium was also mediated at a level of lysosome biogenesis.

The transcription factor MEF2 was recently shown to be important in regulating neuronal survival during differentiation and depolarization (53, 63). p38 mitogen-activated protein kinase also plays a critical role in neuronal survival, since p38 activates MEF2. In contrast to its protective function in neurons, MEF2 promotes apoptosis in T cells. This process is thought to be mediated by enhancing the expression of Nur77 (92). L929 cells had very low levels of MEF2 activity, based on a gel shift assay (data not shown). We did not detect any differences in MEF2 activity between parental and PMCA^mut cells or between TNF-stimulated and nonstimulated cells (data not shown). p38 was activated by TNF treatment, but inhibition of p38 kinase activity did not alter TNF-induced cell death in either parental or PMCA^mut cells (data not shown). Thus, resistance to TNF-induced cell death in PMCA4-deficient cells is unlikely to be related to the p38-MEF2 pathway.

Activation of caspase 8 plays a key role in TNF-induced apoptosis in a number of different types of cells, including MCF-7 cells (74, 78). However, caspase 8 was not involved in TNF-induced death of L929 cells (86). Thus, the lysosomal response during cell death can occur in the absence of caspase 8 activation. In addition, the intracellular calcium concentration seems to have no effect on caspase 8 activation, since treatment of MCF-7 cells with A23187 did not influence TNF-induced caspase 8 activation (data not shown). There are reports suggesting the existence of both caspase-dependent and caspase-independent death pathways (6, 10, 16, 17, 30, 39, 40, 46, 55, 79). Expression of Bax in mammalian cells caused cell death with typical apoptotic phenotypes. Inhibition of caspases by zVAD prevented the cleavage of nuclear and cytosolic substrates of caspases and DNA fragmentation, but failed to prevent the reduction of mitochondrial membrane potential and production of ROS (90).

Interestingly, cell death in the presence of caspase inhibition was associated with cytoplasmic vacuolation (90), which is likely to be the same lysosomal enlargement observed in our experiments. Overexpression of mammalian Bax imposes a lethal phenotype in both Saccharomyces cerevisiae and Schizosaccharomyces pombe (26, 29, 38, 54). The mechanism by which Bax induces yeast cell death is believed to be similar to that in mammals (93). Yeast cells lack caspases, and their death does not show nuclear fragmentation, but rather massive vacuolization of the cytoplasm (93). This is similar to the morphology of human cells expressing Bax in the presence of a caspase inhibitor (90) and L929 cells that were stimulated with TNF. Thus, the signaling pathway that leads to lysosomal responses may be parallel to the caspase pathway. In support of this possibility, there are a number of reports showing that inhibition of caspases blocked nuclear events of apoptosis but not cell death or only delayed cell death (6, 10, 16, 17, 32, 39, 46, 55).

Mitochondria play an important role in many cell death processes (42). Treating L929 cells with TNF in the presence of the ROS scavenger butylated hydroxyanisole inhibited the increase in VAC (data not shown), suggesting that the lysosomal response is downstream of TNF-induced mitochondrial changes. Since there was no detectable cytochrome c released from the mitochondria of TNF-treated L929 cells (24), cytochrome c released from mitochondria may not be required for the lysosomal enlargement observed in our experiments. However, the involvement of other apoptogenic factors released from mitochondria cannot be excluded.

Since programmed cell death is an ordered process, it is likely that lysosomes were responsible for the self-digestion during the course of cell death. Previous studies have provided a large body of evidence suggesting that lysosomes are involved in cell death processes (7, 15, 36, 62, 65, 67, 91). In surveying the literature, we found a number of studies in which increases in the number and size of lysosomes were actually recorded in many electron photomicrographs of apoptotic or necrotic cells, but unfortunately, most of these results have been overlooked (18, 71, 72, 81). The lack of a thorough evaluation of the general involvement of lysosomes in the cell death program is probably related to the lack of a means to evaluate the overall lysosomal changes in cells. The PMCA^mut cell line provided an opportunity for us to evaluate the lysosomal changes that occur during the cell death process and to use VAC as an index for lysosomal change. Our data demonstrate that an increase in VAC is a common event in cell death that occurs in response to diverse stimuli.

Increase of lysosomal volume in processes such as TNF-induced cell death appears to be an execution event, as treatment with A23187 inhibits the increase and can effectively impede cell death after initiation of the apoptotic signal (data not shown). However, in certain cases, lysosomes may be also be involved in the initiation of cell death. Autophagy observed in nerve growth factor-deprived neurons (91) and in tamoxifen-treated MCF-7 cells (8) may represent an initial compensatory response to the absence of a growth signal (13). This may act as both a trigger and an executor of cell death. Translocation of a lysosomal protease, cathepsin B or D, to the cytosol was shown to promote mitochondrial release of cytochrome c and apoptosis (28, 66). Accidental leakage of lysosomes in certain pathological situations or during aging may activate the apoptosis pathway or target other cellular organelles, such as mitochondria, to enhance the death signal (77). This possibility may underlie the observation that lysosome leakage occurs prior to mitochondrial and nuclear events in certain instances of cell death (28, 66).

Taken together, our data suggest a mechanism for the antideath effect of calcium and support the idea that lysosomes are not merely the final station of the endocytic pathway (2). In addition to their housekeeping role, lysosomes can indeed function as suicide bags, as Christian de Duve proposed in the late 1950s (14). Nuclear digestion (DNA fragmentation and nucleus condensation) has been well accepted as an essential event in programmed cell death. Lysosome-mediated cytosolic digestion may be as important as nuclear degradation in the cell death process. The increase in the volume of acidic compartments could be another hallmark of dying cells.