INTRODUCTION

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Control of the amount of translatable mRNA is a common parameter to regulate gene expression in many organisms. The available quantity of an RNA depends largely on the rate of synthesis and/or posttranscriptional processes which control the stability of a transcript by preventing it from degradation. Such posttranscriptional processes have been studied in prokaryotes and different subcellular compartments of eukaryotic cells. In spite of the many variations, some common features behind these processes emerge in all organisms and systems. These include the involvement of mRNA secondary structures and 3' polyadenylation of RNA. While organized RNA secondary structures generally support transcript stabilization, polyadenylation has opposing functions in eukaryotes and prokaryotes.

In the nucleus of eukaryotic cells, pre-mRNAs are polyadenylated at 3' ends that have been generated by endonucleolytic cleavages. These poly(A) tails play important roles in translation initiation and mRNA export from the nucleus and also have a major function in stabilizing mRNAs (27). A completely different function has been attributed to polyadenylation in prokaryotes, where the degradation of mRNA is stimulated by the presence of 3' poly(A) tracts (4). The degradation-promoting effect of polyadenylation was even found in transcripts, which were otherwise protected and stabilized by stem-loop structures (3). A similar effect has also been observed in chloroplasts, where polyadenylation also accelerates the decay of mRNA (15, 25).

Polyadenylation has also been found in mitochondria of various organisms. The addition of adenosines to mRNAs in mammalian mitochondria has been suggested to create functional translation stop codons, which are not encoded in the DNA (22). Short oligo(A) tails were also detected at the 3' ends of RNA in yeast mitochondria. These are about 8 nucleotides (nt) long and predominantly found at the large rRNAs (29). Similarly, oligo(A) tracts were found at some RNA species of the fragmented rRNA in Plasmodium falciparum (12). While the function of the oligo(A) tails in yeast is still unclear, those in Plasmodium were suggested to protect these RNA fragments from exonucleolytic digestion. Recently, polyadenylation was also reported for RNAs in plant mitochondria. In sunflower a dicistronic mRNA associated with cytoplasmic male sterility was suggested to be specifically destabilized by polyadenylation in fertility-restored lines (10). In maize, polyadenylation was detected at multiple sites of the cox2 transcripts. As in sunflower, poly(A) tracts were indicated to decrease mRNA stability, although the effect observed in maize mitochondrial extracts was very weak (19). We report here nonencoded nucleotides at the 3' ends of pea and Oenothera mitochondrial transcripts containing double inverted repeats in their 3' untranslated regions (UTRs). These structures have been suggested to increase the stability of the RNA, and the stem-loop structures of pea atp9 transcripts as well as of orf138 mRNAs in Brassica were found to act as processing signals and/or stability elements at least in vitro (1, 5, 6, 16, 23). In this report special emphasis was dedicated to the interaction of the stabilizing effect of such double stem-loop structures and degradation-enhancing polyadenylation. In addition to adenosines, nonencoded cytidines and, to a lesser extent, uridines were detected in vivo. The combination of nonencoded cytidines with adenosines may indicate an involvement of the tRNA terminal nucleotidyltransferase in the addition of nonencoded nucleotides to 3' ends of plant mitochondrial mRNAs.

	MATERIALS AND METHODS

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3' RACE analysis of mtRNA. The 3' rapid amplification of cDNA ends (RACE) was carried out in principle as described by Frohmann et al. (9). First-strand synthesis was initiated on 5 µg of total pea mitochondrial RNA (mtRNA) with an oligo(dT)₁₇-adapter primer (DTXSC) using Superscript II reverse transcriptase (RT) (GIBCO-BRL) under conditions given in the manufacturer's manual. About one-fourth of the total cDNA pool was used as the template in a PCR with adapter primer XSC and atp9-specific primer PA-1 (349 to 329) with KlenTaq polymerase in a buffer supplied by the manufacturer (Clontech). After 35 cycles (30 s at 94°C, 1 min at 40°C, and 1 min at 68°C), PCR fragments were size fractionated on a 1.5% agarose gel and distinct products with sizes of 0.3, 0.4, 0.8, and 1.0 kb were observed above a smear ranging from about 0.2 to 2.0 kb. Since the PCR fragments generated from pea atp9 transcripts, which are polyadenylated downstream of the double stem-loops, are expected to be at least 665 bp long, cDNA fragments between 0.5 and 0.9 kb in length were eluted from the gel and used as templates in a PCR with primers XSC and PA-10, a nested atp9-specific primer (98 to 84 with a 5'-attached EcoRI restriction site). After 35 cycles (30 s at 94°C, 1 min at 50°C, and 1 min at 68°C), reaction products were digested with SalI and EcoRI and run on an agarose gel. cDNA fragments between about 0.35 and 0.55 kb long including the expected fragment of about 0.43 kb, were eluted from the gel and cloned into pBluescript vectors (Stratagene) following standard protocols (24). In an analogous approach, oligo(dT)-adapter primer DTXSC and adapter primer XSC were replaced by DTNSX-1 and NSX-1 oligonucleotides, and atp9-specific primers PA-10 and PAX-1 (+259 to +274 with a 5'-attached XbaI restriction site) were used. cDNA clones generated with the NSX primer set carry NsiI restriction sites immediately downstream of the poly(A) tracts. Linearization at this site allows the in vitro transcription of mRNAs ending with poly(A) tracts.

Direct ligation of anchor primers to 3' ends of in vitro processing products and steady-state mtRNA. In vitro processing assays were carried out under standard conditions, and reaction products were separated on a 6% polyacrylamide gel. Radioactive bands were visualized by autoradiography and were excised from the gel. The RNAs were eluted from the gel in a buffer containing 0.5 M ammonium acetate, 0.1 mM EDTA, and 0.1% sodium dodecyl sulfate. After phenol-chloroform extraction, the in vitro processing products were ethanol precipitated and ligated to 20 pmol of anchor primer (RiboNSX) in the presence of 20 U of RNA ligase (GIBCO-BRL), 1% (vol/vol) dimethyl sulfoxide, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl₂, 10 mM dithiothreitol, 0.25 mM ATP, and 0.6 µg of bovine serum albumin per µl. The ligation reaction was performed for 24 h at 15°C in a 10-µl total reaction volume. Following two rounds of phenol-chloroform extraction, nonligated anchor primers were removed by centrifugation in Microcon 30 microfiltration units at 14,000 × g to a final volume of 10 µl. First-strand synthesis was primed with oligonucleotide NSX-1 using the complete ligation reaction as the template and Superscript II RT. Subsequent amplification with primers PAX-1 and NSX-1 was performed with KlenTaq polymerase (Clontech) in a buffer supplied by the manufacturer. After 35 cycles (1 min at 94°C, 1 min at 50°C, and 30 s at 68°C), PCR products were digested with XbaI and SalI and were cloned into pBluescript vectors. Respective clones were identified in a colony hybridization using Hybond-N membranes (Amersham Pharmacia Biotech) and oligonucleotide PA-17hyb (+266 to +284) as probe, following a protocol given by the manufacturer.

Lysate preparation and in vitro processing reactions. Pea mitochondrial lysate preparation and the in vitro processing reactions were performed as described previously (6).

DNA templates and in vitro transcription. Polyadenylated pea atp9 precursor molecules are transcribed from cDNA clone 9.17 generated in the 3' RACE approach with primers PAX-1 and NSX-1 on in vitro-transcribed RNA obtained from clone no. 9. Plasmid 9.17 was linearized with NsiI and transcribed with T7 RNA polymerase. The resulting transcript (117 nt) contains the pea atp9 double stem-loop and 43-nt upstream and 23-nt downstream sequences. The upstream part corresponds to vector sequences from the T7 transcription start point to the XbaI restriction site. The downstream moieties comprise 21 adenosines at the 3' end. Transcripts containing (A)₃ or (A)₁₀ poly(A) tracts downstream of the inverted repeat are directly transcribed from DNA fragments obtained by amplification with primer M13 universal and primers IR⁺dT₃ [(A)₃] and IR⁺dT₁₀ [(A)₁₀] on clone 9.17 as the DNA template.

Nucleic acids. Pea and Oenothera mtRNAs were isolated from respective organelles enriched by differential centrifugation and purified on Percoll (Amersham Pharmacia Biotech) gradients as described previously (2). Sequences of the primers used are available on request. All oligonucleotides were purchased from GIBCO-BRL.

Miscellaneous methods. Sequencing reactions were carried out with the Thermo Sequenase fluorescent labeling kit (Amersham Pharmacia Biotech) according to instructions given by the manufacturer. All standard methods were used as described by Sambrook et al. (24).

	RESULTS

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Poly(A) tails at the 3' ends of atp9 mRNAs in pea mitochondria. The pea atp9 gene is transcribed into three different mRNA species with differing 5' termini but identical 3' ends. These 3' termini were mapped in two independent S1 protection analyses within the 3' part of the second of two consecutive inverted repeats and immediately downstream thereof (6, 21). Recently it was found that this double inverted repeat with the potential to fold into a double stem-loop structure does not terminate transcription in vitro but rather functions as a processing signal and stabilizing element during a controlled 3' processing event (5, 6). To investigate whether nonencoded poly(A) tails are present at these transcript termini, a 3' RACE analysis, including two rounds of PCR with an oligo(dT)-adapter primer and two nested atp9-specific primers, was performed with total pea mtRNA (for details see Materials and Methods). The sequence analysis of 18 clones indicated the presence of nonencoded poly(A) tails at the 3' ends of these mRNAs. In four clones, polyadenylation sites are located at positions +6 and +2 relative to the first nonpaired nucleotide (+1) downstream of the second stem (Fig. 1). The majority of the poly(A) tracts are found at positions 2 to 5 within the downstream part of the second stem. These polyadenylation sites coincide with previously mapped 3' ends. Three clones contained poly(A) sequences attached within the second loop, while two others are added at the beginning and end of the upstream part of the second stem, respectively. The lengths of the poly(A) stretches vary between 14 and 25 adenosines, with 10 of the 18 clones containing more than the 17 adenosines derived from the oligo(dT)-adapter primer. In four clones, nonencoded cytidine, guanosine, and thymidine (corresponding to uridine in the RNA) nucleotides are found predominantly in the 5' part of the attached sequences. Almost all clones investigated are edited at position +20 (relative to the translation start codon [+1]), while a cytidine-to-uridine alteration at position +50 is found less frequently. Both editing events change the codon identity from serine to leucine (data not shown).

View larger version (36K): [in this window] [in a new window]   FIG. 1.   3' RACE analysis of pea mitochondrial atp9 transcripts. (A) Previously mapped 3' ends are indicated within the pea atp9 potential double stem-loop structure (arrows). (B) Nucleotide sequences of 3' RACE clones containing poly(A) tails. Nonencoded nucleotides found in 18 analyzed cDNA clones (designations are shown on the left) are given in lowercase letters. The positions of the polyadenylation sites (indicated above the sequences) correspond to the 3'-most nucleotide. Numbering refers to the first nucleotide (+1) downstream of the inverted repeats (indicated by oppositely oriented grey shadowing arrows) in the genomic sequence (gen). Vertical arrows beneath the genomic sequence indicate previously mapped 3' ends. (C) The numbers of clones identified are given for each polyadenylation site.

Potential poly(A) tracts in Oenothera atp1 transcripts. The analysis of Oenothera atp1 transcripts extends this study to other double-stem-loop-containing mRNAs from mitochondria of another plant species (Fig. 2). Amplification with an atp1-specific primer and an adapter primer on a total cDNA pool primed with an oligo(dT)-adapter primer generated a dominant product of about 0.45 kb expected of nonencoded poly(A) sequences attached downstream of the stem-loop structure (Fig. 2B). This fragment was eluted from the gel and was either used as a template in a second PCR with a nested, atp1-specific primer and an adapter primer or directly cloned. Sequencing of 10 cDNA clones obtained from both amplification reactions revealed poly(A) tracts attached at positions +5, +4, and +3 immediately downstream of the double stem-loop structure. These polyadenylation sites are consistent with previously mapped 3' ends (26). Only a single clone indicated a polyadenylation site at position 3 within the downstream part of the second stem (Fig. 2C). These results show that the distribution of the polyadenylation sites in Oenothera atp1 mRNAs is less variable than in pea atp9 transcripts. The lengths of the poly(A) tails observed in the Oenothera atp1 cDNA clones range from 16 to 23 adenosines with only a single guanosine insertion within a poly(A) sequence.

View larger version (28K): [in this window] [in a new window]   FIG. 2.   Identification of potential polyadenylation sites in Oenothera atp1 transcripts. (A) Potential double stem-loop structure found in the 3' UTR of Oenothera atp1 mRNAs. Previously mapped 3' termini are indicated by arrows. (B) DNA fragments generated in a PCR with adapter primer (NSX) and an atp1-specific oligonucleotide (OeatpA-RI) on oligo(dT)-adapter-primed cDNA are size fractionated on a 1.5% agarose gel (lane P). Besides minor fragments of about 1.0, 0.85, and 0.2 kb, a strong cDNA fragment (0.45 kb) corresponding to RNAs polyadenylated downstream of the stem-loop is detected. Lengths of coelectrophoresed DNA marker fragments (lane M) are given in kilobase pairs on the left. (C) Partial sequences of Oenothera atp1 cDNA clones containing nonencoded poly(A) tails (indicated in lowercase letters). Designations and numbering are analogous to those for Fig. 1. (D) Correlation of the number of clones with poly(A) addition sites.

Rapid and efficient processing of polyadenylated pea atp9 transcripts in vitro. The results of the 3' RACE analyses identified nonencoded poly(A) tracts at the 3' ends of transcripts containing double stem-loop structures in their 3' UTRs. Such poly(A) tails were recently suggested to decrease mRNA stability in plant mitochondria (10, 19). This destabilizing effect would counteract the function of the double stem-loop, which was found to act as a processing signal and stability element at least in vitro (5, 6). To solve this question, the consequence of poly(A) tails on double-stem-loop-containing mRNA was tested in vitro. Nonpolyadenylated synthetic transcripts (96 nt) used in these assays were transcribed from a T7 promoter-containing PCR product (Fig. 3A) and corresponded to the standard substrate shown to be correctly processed in a pea mitochondrial in vitro system (6). It contains the pea atp9 double stem-loop structure and 21 and 28 original mitochondrial nt upstream and downstream, respectively. In the polyadenylated mRNA (113 nt), the 3' trailer downstream of the stem-loop structure consists of a 21-mer adenosine homopolymer downstream of two mitochondrially encoded nucleotides while the upstream leader (42 nt) represents pBluescript vector sequences. Details of the construction of the DNA templates for transcription of these substrates (Fig. 3B) are given in Materials and Methods. To test the influence of the regions upstream of the inverted repeat, an analogous RNA substrate, which contains original mitochondrial downstream sequences (28 nt) and the alien sequences deriving from the vector (42 nt), was also tested in vitro. After incubation of the nonpolyadenylated standard transcript for 60 min, a single processing product of about 70 nt was observed (Fig. 3C, left). This product corresponds to an RNA species where the downstream part is removed, as seen in previous processing experiments (6). An accelerated generation of processing products is observed upon incubation of polyadenylated substrates. After 10 min, two processing products of about 70 and 80 nt are generated. While the smaller product has a size consistent with a 3' end similar to that of the product obtained in the reaction with the nonpolyadenylated standard substrate, an additional RNA, about 5 to 10 nt larger, is generated. Longer incubation (for 60 min) resulted in the generation of two products that seem to be slightly smaller than those observed after 10 min (Fig. 3C, right). A product very similar to the one generated from the nonpolyadenylated standard substrate and to the smaller one (70 nt) deriving from the polyadenylated substrate was observed in in vitro tests with the nonpolyadenylated substrate, with vector sequences upstream of the stem-loop structure. The temporal dynamic of the product formation is identical to that of the processing reaction of the nonpolyadenylated standard substrate, which indicates that sequences upstream of the stem-loop structure have no degradation-promoting function (data not shown).

View larger version (17K): [in this window] [in a new window]   FIG. 3.   In vitro processing of polyadenylated and nonpolyadenylated pea atp9 precursor molecules. (A) Generation of the pea atp9 standard transcript containing a double inverted repeat (grey horizontal arrows). The substrate is transcribed in vitro (IVT) from a PCR product obtained with primers PA-12 and T7IVR+, which contains a promoter for T7 RNA polymerase (T7P, hatched box). (B) The polyadenylated substrate is transcribed from the linearized clone 9.17, obtained by RT-PCR cloning of a polyadenylated pea atp9 mRNA with primers NSX-dT (adapter primer for cDNA synthesis), PAX-1, and NSX-1 (PCR). (C) Identical amounts of substrates (about 12,000 cpm) with [poly(A)+] and without [poly(A)] poly(A) tracts are incubated for 10 and 60 min in a pea mitochondrial lysate. Resulting reaction products are separated on a 6% polyacrylamide gel (lanes 10 and 60). Control reactions (lanes C10 and C60) are performed under identical conditions without protein. Lengths of coelectrophoresed DNA marker fragments are given in nucleotides (Nt) in the right margin. Discrepancies between the sizes of RNA molecules and the DNA marker fragments are due to different migration velocities of RNA and DNA molecules.

View larger version (21K): [in this window] [in a new window]   FIG. 4.   Precise determination of 3' termini of in vitro processing products. (A) The in vitro processing products (b to f, indicated by dotted boxes) obtained from nonpolyadenylated [poly(A)] and polyadenylated [poly(A)+] substrates with inverted repeats (IR+) were excised, and the RNA molecules were eluted from the gel. The 3' ends of the different products were analyzed by direct anchor primer ligation followed by RT-PCR analysis. Lanes 10 and 60 represent results at 10 and 60 min; lanes C10 and C60 represent control reactions at 10 and 60 min. (B to F) Secondary structures of the pea atp9 double stem-loop with nonpolyadenylated (B) and polyadenylated (C to F) downstream moieties. Arrows indicate the 3' terminal nucleotide found in individual clones. The numbers of cDNA clones containing certain 3' ends are given at the arrows. Designations of the individual stem-loops (B to F) correspond to the designations of the analyzed processing products (b to f).

Temporal dynamics of the in vitro processing of polyadenylated pea atp9 transcripts. The generation of an additional distinct product from polyadenylated transcripts raises the possibility that this could be an intermediate processing product. To investigate the temporal course of the in vitro processing of polyadenylated pea atp9 mRNAs (Fig. 1B), the generation of processing products was followed over a period of 90 min (Fig. 5). Already after 1 min, diffuse smaller RNAs appear, indicating the initiation of the processing reaction, which results in the detectable formation of the approximately 90- to 95-nt-long RNA fragment after 2.5 min. The smeared RNA between the substrate and this product indicates exonucleolytic processivity. This impression is substantiated by the continuously decreasing size of this 90- to 95-nt-long RNA, with several nucleotides being consecutively removed upon prolonged incubation. A similar effect is also seen with the 80- to 85-nt-long product, which first appears after 5 min. The substrate and the two processing products show distinct dynamics in their respective abundances. As expected, the substrate decreases continuously over 90 min. The larger product (90 to 95 nt) has its highest abundance after 10 min, while the smaller product (80 to 85 nt) reaches maximum abundance after 60 min. These results suggest that the large processing product, whose 3' ends map immediately downstream of the double stem-loop, is a stabilized intermediate in the exonucleolytic generation of the small product, with its 3' ends located within the downstream part of the second stem. Thus, an exoribonuclease seems to remove the poly(A) tails very rapidly but stalls or falls away from the RNA either at the 3' end of the stem-loop structure or simply at the transition from the poly(A) stretch to the "normal" sequence. The smaller product most likely results from a second steric hindrance within the downstream part of the stem.

View larger version (79K): [in this window] [in a new window]   FIG. 5.   Time course of the in vitro processing of a polyadenylated pea atp9 precursor. A polyadenylated pea atp9 substrate is incubated over a period of 90 min, and probes are taken at 0 to 90 min, as indicated above the lines. Control reactions are performed under identical conditions in the absence of protein for 0 (C0) and 90 (C90) min. Sizes of coelectrophoresed DNA marker fragments are given in nucleotides (Nt).

Accelerated total degradation of RNA substrates with no inverted repeat. The results presented above indicate that polyadenylation accelerates RNA processing of stem-loop-containing RNAs, while the total degradation of the transcripts remains almost unaffected. To investigate the stabilizing function of this stem-loop structure during the formation of the different processing products, polyadenylated and nonpolyadenylated RNAs without a stem-loop were tested in the in vitro processing system. The substrate without a stem-loop structure and poly(A) tract, representing 101-nt-long RNA molecules with 3' ends just upstream of the first inverted repeat (Fig. 6A), is consecutively degraded without formation of any stable intermediate, indicating that the stem-loop is indeed responsible for the generation of stabilized products (Fig. 6B, left). A different processivity is seen with polyadenylated transcripts. Here the presence of a poly(A) tract in the substrate significantly accelerates the total turnover of the precursor RNA (Fig. 6B, center). Only a weak signal indicates the presence of an intermediate, which corresponds in size to the RNA without a poly(A) tail. The low intensity of this signal indicates only weak stability of this product.

View larger version (26K): [in this window] [in a new window]   FIG. 6.   Accelerated degradation of RNAs without stem-loops containing 3' poly(A) tails. (A) Substrates with no inverted repeat containing no 3' homopolymer tract (IR) and a 3' poly(A)21 [IR poly(A)+] are transcribed in vitro (IVT) from PCR products with primer T7IR containing a T7 promoter (T7P, hatched box) and primers PIR and IRdT (A)21, respectively. (B) Incubation of the respective substrates with pea mitochondrial protein extracts for 10 and 60 min. Designation of the lanes (10 and 60) corresponds to the length of incubation. Control reactions are performed in the absence of protein under otherwise identical conditions for 10 (C10) and 60 (C60) min. The sizes of DNA marker fragments are indicated in nucleotides (Nt).

Detection of nonencoded nucleotides at 3' transcript termini of steady-state RNA. The 3' RACE analysis using an oligo(dT)-adapter primer does indicate the presence of poly(A) sequences at the 3' ends of mtRNAs. Several questions are, however, left unanswered in this approach. First, the lengths of the poly(A) tails cannot be determined, and second, the majority of other nonencoded nucleotides (i.e., C, G, or U) might remain undetected. This may be a major drawback, since, for example, considerable uridylyltransferase activity has been detected in plant mitochondrial protein extracts (S. Binder, unpublished results).

View larger version (71K): [in this window] [in a new window]   FIG. 7.   A direct anchor primer ligation-RT-PCR analysis identifies nonencoded nucleotides at pea atp9 steady-state transcripts. (A) Scheme of the direct anchor primer ligation-RT-PCR approach as outlined in Materials and Methods. (B) Ethidium bromide-stained agarose gel with DNA fragments obtained from a PCR carried out with primers PA-10 and NSX-1. Sizes of DNA marker fragments are given in kilobase pairs on the right. (C) Sequences of the downstream inverted repeat of the pea atp9 3' UTR (indicated by grey shading) obtained from a direct anchor primer ligation-RT-PCR approach. Nonencoded nucleotides are given in lowercase letters. Previously mapped 3' transcript termini are indicated by vertical arrows beneath the genomic sequence (gen). The numbering of the sequence is given with respect to the first nucleotide (+1) downstream of the inverted repeat. (D) Number of clones containing encoded 3' terminal nucleotides and clones with nonencoded nucleotides found at certain positions relative to the first nucleotide downstream of the inverted repeat (+1).

Poly(A) tracts with 10 or more adenosines stimulate RNA processing and degradation. The anchor primer ligation-RT-PCR analysis detects only short nonencoded extensions up to 3 nt. Two explanations are possible for such relatively short tracts. Either the extensions per se are indeed only a few nucleotides long, or they are the result of the processing reaction. To test the latter assumption, substrates with no inverted repeat containing 0, 3, 10, or 21 additional adenosines were incubated with the pea mitochondrial lysate. While both 10 or 21 oligo(A) tails significantly accelerate degradation, no difference is observed in the processing of substrates with 3 or 0 additional adenosines (Fig. 8A). An analogous effect is seen with substrates containing inverted repeats. An extension of 3 adenosines has no impact on processing, whereas a processing product is rapidly formed from a precursor with 10 adenosines (Fig. 8B).

View larger version (45K): [in this window] [in a new window]   FIG. 8.   Oligo(A) tails with 10 or more adenosines promote RNA degradation or processing. (A) Incubation of synthetic transcripts with no inverted repeat with either no extension (IR), a 3-nt extension [IR(A)3], a 10-nt tract [IR(A)10], or a 21-nt tail [IR(A)21] are incubated with a pea mitochondrial lysate for 10 and 60 min. Clear differences are observed after 60 min with strong degradation-stimulating effects for the tails with 10 and 21 adenosines. In comparison, no significant differences in the decay are observed in reactions with substrates without extensions or with three additional terminal adenosines. Nt, nucleotides. (B) Substrates containing the pea atp9 inverted repeat with 3 [IR+poly(A)3] or 10 [IR+poly(A)10] adenosines attached 2 nt downstream of the second stem were incubated with a pea mitochondrial lysate. The product generated from the latter substrate where sequences downstream of the stem-loops have been removed clearly indicates a processing-promoting effect of the 10-adenosine extension. In comparison, only a minor shortening of the substrate with three terminal adenosines is observed after 10 and 60 min (lanes 10 and 60). No degradation or processing is observed after incubation of the substrates for 10 and 60 min in the absence of protein (lanes C10 and C60). Nt, nucleotides.

	DISCUSSION

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3' nonencoded nucleotides in plant mitochondrial transcripts. Two independent approaches were used to identify nonencoded nucleotides at the 3' ends of plant mitochondrial transcripts. While the 3' RACE analysis initiated with an oligo(dT)-adapter primer indirectly shows the presence of nonencoded adenosines at the 3' transcript termini, the direct anchor primer ligation-RT-PCR approach provides straightforward evidence for the existence of such nonencoded 3' extensions without any inherent experimental bias for certain nucleotides.

Function of polyadenylation in plant mitochondria. Recent reports of polyadenylation of plant mitochondrial transcripts suggested that the presence of 3' poly(A) tails accelerates the degradation of mRNAs (10, 19). Our results obtained for transcripts without a stem-loop structure are consistent with these results. A significant acceleration of RNA decay is caused by the presence of poly(A) tails in these transcripts. Surprisingly, an even stronger destabilizing effect is observed with poly(G)₂₁ tails, which contradicts previous reports on chloroplasts and cannot be rationally explained at present (7, 8; data not shown). No effects of poly(G) stretches were detected in transcripts of nuclear reporter genes in plants, indicating that indeed the effects of poly(G) stretches might differ for different compartments of the plant cell (13).

How are the 3' nonencoded nucleotides added to mRNAs? The presence of nonencoded nucleotides at the 3' ends of mRNAs raises the question of how these nucleotides are added. In other compartments of plant cells and in other organisms, poly(A) tails are generally synthesized by poly(A) polymerase, which has so far not been reported in plant mitochondria. It has recently been shown that this protein from Escherichia coli incorporates all 4 nt into the 3' extensions, at least in vitro, so one can speculate that an analogous enzyme activity may add the nonencoded nucleotides to mRNA 3' ends in plant mitochondria (28).