INTRODUCTION

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The mammalian high-mobility group I (HMGI) family of chromosomal proteins includes HMG-I and HMG-Y, which are coded for by the same gene, HMGI(Y), through alternative splicing (23), and the closely related HMGI-C protein (27). The HMGI proteins are involved in the regulation of chromatin structure and function (25). While not typical transcriptional activators, HMGI(Y) proteins are required for the expression of many eukaryotic genes. These proteins bind adenine- and thymine-containing sequences located in the minor groove of DNA. Their DNA-binding domain is located in the N-terminal region of the protein and contains three short basic repeats, the so-called AT hooks. HMGI(Y) DNA-binding sites have been identified in many promoters, e.g., interleukin-4 (13), interleukin-2 receptor -chain (22), lymphotoxin (15), and the human papovavirus JC (24) genes. These sites are often close to the DNA-binding sites of known transcription factors like NF-B (38) and Tst-1/Oct-6 (24) and appear critical for viral induction of the human beta interferon gene (14, 38, 39). HMGI(Y) also interacts directly with several transcription factors. In fact, it binds to the basic leucine zipper region of the activating transcription factor 2, thus promoting its dimerization and binding to the beta interferon promoter (14).

HMGI-C gene knockout mice show a pygmy phenotype with a reduction of the adult body weight, mainly affecting fat tissue (49). The fat index, a reliable indicator of the total fat content relative to body weight, is approximately eight times lower in pygmy mice than in the wild-type littermates. Furthermore, the regulation of HMGI-C in vivo modulates obesity in a mouse model, and rearrangements of the HMGI-C and the HMGI(Y) genes have been found in human lipomas carrying chromosomal translocations involving the regions 12q13-14 and 6p21, respectively (2, 34, 40). Our group has recently demonstrated that the HMGI-C rearrangement plays a critical role in the generation of lipomas. In fact, transgenic mice carrying a truncated HMGI-C gene, which contains only the three AT hook domains, develop a giant phenotype and predominantly abdominal and pelvic lipomatosis (4). These observations, taken together, implicated HMGI(Y) proteins in adipogenesis. To elucidate further the mechanism of action of HMGI(Y) in adipogenesis, we used mouse 3T3-L1 fibroblasts as a model system. These cells differentiate into adipocytes upon treatment with specific agents (35). Adipocyte differentiation involves a group of transcription factors, CCAAT/enhancer-binding proteins (C/EBPs) (5, 29, 33, 42, 46), which are expressed at specific stages of adipogenesis. Hormonal stimulation causes C/EBP and C/EBP levels to increase and induce the expression of the transcription factor peroxisome proliferator-activated receptor gamma (44). This factor, in turn, leads to an increase of C/EBP, which promotes the induction of several adipocyte-specific genes, including that for the fatty acid-binding protein 422/aP2 (11, 12) and the obese gene, which encodes leptin (21). Here we report the following: (i) HMGI(Y) gene expression increases during adipocytic conversion of 3T3-L1 cells; (ii) blockage of HMGI(Y) synthesis stimulates cell growth and impairs 3T3-L1 differentiation; (iii) HMGI(Y) physically interacts with C/EBP in vivo and in vitro; and (iv) HMGI(Y) proteins greatly enhance the C/EBP-mediated transactivation of the leptin promoter.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

Cell culture, transfections, and plasmids. The mouse NIH 3T3-L1 cells used in this study were generously donated by E. Santos (National Cancer Institute, National Institutes of Health, Bethesda, Md.). Cell cultures were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% calf serum (GIBCO BRL, Life Technologies, Gaithersburg, Md.). Induction of adipocytic differentiation in 3T3-L1 cells was performed essentially as described elsewhere (35). Briefly, confluent 3T3-L1 cells were grown in DMEM supplemented with 10% calf serum until confluency was reached. Two days later, they were grown in DMEM supplemented with 10% fetal calf serum, 0.5 mM 1-methyl-3-isobutylxanthine, 10⁶ M dexamethasone, and 10 µg of insulin/ml for 48 h. Cells were further cultured in the same culture medium devoid of dexamethasone and methylisobutylxanthine for 6 days. The 3T3-L1 fibroblasts were transfected by the calcium phosphate technique (18). Transfected cells were subjected to G418 selection (400 µg/ml). 293 cells were maintained in DMEM medium containing 10% fetal calf serum (GIBCO BRL, Life Technologies) and transiently transfected for in vivo binding assays or luciferase assays as described above. A 1,500-bp cDNA including the HMGI(Y) gene was subcloned into the HindIII site of the expression vector pRc/CMV (Invitrogen). A 489-bp cDNA fragment corresponding to the entire coding sequence of the HMGI(Y) gene was subcloned into the HindIII and XbaI sites of the expression vector pRc/CMV (Invitrogen) in the antisense orientation. Expression of the sense and antisense HMGI(Y) RNA was achieved by reverse transcription (RT)-PCR: the forward primer for the sense vector, designed on the ATG sequence of the HMGI(Y) gene, was as follows: 5'-AGGAGAATGAGCGAGTCG-3'. The reverse primer, designed on the Sp6 sequence of the cytomegalovirus (CMV) vector, was as follows: 5'-AGTCGAGGCTGATCAGCGAG-3'. The forward primer for the antisense vector, designed on the stop codon sequence of the HMGI(Y) gene, was as follows: 5'-CTGCGAGTGGTGATCACT-3'. The reverse primer, designed on the Sp6 sequence of the CMV vector, was as follows: 5'- AGTCGAGGCTGATCAGCGAG-3'. The p(161)ob-luc plasmid, containing 161 bp of the obese gene promoter driving a luciferase gene, and the m52 mutant, in which the C/EBP binding motif has been disrupted, are also described elsewhere (20).

Growth curves. For standard growth curves, cells were seeded at a density of 10⁵ in 60-mm-diameter plates (Falcon) and grown in DMEM supplemented with 10% calf serum (GIBCO). Medium was renewed every 2 days, and cells were counted (see Fig. 4A). The doubling time was measured when the cells were in the logarithmic phase of growth: for 3T3-L1 and 3T3-L1-HMGI(Y)s cells, it occurred between days 3 and 4, and for 3T3-L1-HMGI(Y)as cells, it occurred between days 4 and 5 of culture. For growth curves of differentiating cells, cells were seeded and grown as described above until confluent. Two days after reaching confluence, cells were induced to differentiate according to the standard protocol (see above). Starting from 1 day after induction of differentiation, cells were counted (see Fig. 4B).

Northern blot analysis. Total RNA was extracted with RNAzol (Tel-Test, Inc., Friendswood, Tex.) according to standard procedures (32). Northern blotting and hybridizations were carried out as previously described (32). The HMGI probe was derived from pHMGI(Y) (23). A 0.4-kb EcoRI-HindIII fragment corresponding to the cDNA of the constitutively expressed enzyme human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used to control equal RNA loading. Quantification of the hybridization signal was performed using a Molecular Dynamics PhosphorImager. The images recorded by the PhosphorImager were analyzed by volume integration with the ImageQuant software.

RT-PCR analysis of the expression of the adipocyte differentiation markers. Total RNA, digested with DNase, was reverse transcribed using random exonucleotides as primers (100 mM) and 12 U of avian myeloblastosis virus reverse transcriptase (GIBCO). Subsequent PCR amplification was as follows: 200 ng of cDNA was amplified in a 25-µl reaction mixture containing Taq DNA polymerase buffer, 0.2 mM deoxynucleoside triphosphates, 1.5 mM MgCl₂, 0.4 mM concentrations of each primer, and 1 U of Taq DNA polymerase (Perkin-Elmer-Cetus, Branchburg, N.J.). The PCR amplification was performed for 30 cycles (94°C for 30 s, 55°C for 2 min, and 72°C for 2 min). The primers used for aP2 gene expression were 5'-GATGTCAGCAGGAAGTCACC-3' and 3'-CGAAGGAGGTTTAGCAAGAG-5', corresponding to nucleotides 109 to 138 and nucleotides 427 to 408 (41). For the obese gene expression the sequences of the primers used were 5'-CCTGCTCCAGCAGCTGCAAG-3' and 5'-GAGGAAAATGTGCTGGAGACCC-3', which map on exon 1 and exon 2, respectively, and give rise to a specific 195-bp product (20). In addition, a set of primers specific for GAPDH was added to each reaction after 20 cycles of PCR to serve as an internal control for the amount of cDNA tested. The GAPDH-specific primers were the following: 5'-ACATGTTCCAATATGATTCC-3' (forward), corresponding to nucleotides 194 to 214, and 5'-TGGACTCCACGACGTACTCAG-3' (reverse), corresponding to nucleotides 336 to 356. The reaction products were analyzed on a 2% agarose gel and then transferred by blotting to GeneScreen Plus nylon membranes (Dupont, Boston, Mass.). The membranes were hybridized with an HMGI(Y) cDNA probe (7, 23). The relative levels of aP2 and ob expression were assessed by comparison with the level of GAPDH in the same sample.

Immunoblotting and immunoprecipitation. Protein extracts were prepared from terminally differentiated or undifferentiated fibroblasts as previously described (6). The following antibodies were used for immunoprecipitation and Western blotting: anti-C/EBP (C-19) rabbit polyclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, Calif.) and anti-HA 12CA5 mouse monoclonal antibodies (Boehringer, Mannheim, Germany). The rabbit polyclonal antibodies directed against the HMGI(Y) proteins already have been described (7, 8). For Western blot experiments, equal amounts of protein lysates were loaded, as demonstrated by staining of the membranes with Ponceau Red. To confirm equal loading, the same Western blots were incubated with antibodies to -tubulin (Sigma-Aldrich Corporation, St. Louis, Mo.). For coimmunoprecipitation experiments, antigens and antibodies were incubated for 1 h before the addition of protein A-Sepharose beads (Pharmacia Biotech, Uppsala, Sweden). After another 1 h, the beads were collected and washed five times with lysis buffer. The beads were then boiled in sodium dodecyl sulfate (SDS) loading buffer for immunoblotting analysis. The protein extracts separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) were transferred to Immobilon-P transfer membranes (Millipore). Membranes were blocked with 5% nonfat milk proteins and incubated with antibodies at the appropriate dilutions. Bound antibodies were detected by the appropriate horseradish peroxidase-conjugated secondary antibodies followed by enhanced chemiluminescence (Amersham).

In vitro and in vivo binding assays. For in vitro binding assays, the HMGI(Y) cDNA was expressed as a glutathione S-transferase (GST) fusion protein in bacteria as described previously (10). Briefly, a 900-bp EcoRI-BamHI fragment generated by PCR and including the complete coding sequence was subcloned in pGEX2T. The GST-HMGI(Y) construct was used to transform Escherichia coli strain BL21. Bacterially expressed GST and GST-HMGI(Y) proteins were bound to glutathione-agarose (Sigma-Aldrich Corporation). The beads were washed, and the size and purity of the bound protein were evaluated by Coomassie staining of an SDS-polyacrylamide gel. Equal amounts of GST and GST-HMGI(Y) proteins (5 µg) were used for binding assays. C/EBP cDNA was obtained by PCR amplification and cloned in the pBluescript vector (Stratagene, La Jolla, Calif.). Transcription and translation reactions were performed with the T7-rabbit reticulocyte lysate kit (Promega, Madison, Wis.) as suggested by the manufacturer. The in vitro-translated C/EBP was allowed to associate with glutathione-agarose-bound GST or GST-HMGI(Y) for 2 h in lysis buffer (6) at 4°C. The pellets were washed four times in lysis buffer, and the proteins were dissociated by boiling in loading buffer and electrophoresed on a 10% polyacrylamide-SDS gel. The proteins were transferred to Immobilon-P, and C/EBP was visualized as described above. For in vivo binding assays, 293 cells were transfected as described by Graham and van der Eb (18). Cells were transfected with 5 µg of each plasmid, and carrier DNA was added to a total of 10 µg. Cells were harvested 36 h after transfection, and protein extracts were prepared as described above. Extracts were immunoprecipitated and immunoblotted with the indicated antibodies.

Transient transfection and luciferase activity assay. Transfections into 293 cells were performed by calcium phosphate precipitation (18). Cells were transfected with 5 µg of p(161)ob-luc, m52, or RSV-luc reporter plasmids together with 1 µg of pHMGI(Y)s. The hemagglutinin (HA)-tagged HMGI(Y) wild-type and deletion mutants were generated by PCR, sequenced, and subcloned into the pCEFL vector. One microgram of pSV2CAT plasmid was cotransfected to demonstrate equal transfection efficiency in the cell lines tested, and chloramphenicol acetyltransferase activity was measured by thin-layer chromatography with 95% chloroform-5% methanol. Cells were harvested 24 h after transfection, and luciferase activity was measured with a luminometer (Lumat LB9507; Berthold). The relative activities were calculated by dividing the normalized activities by the activity of the m52 and the Rous sarcoma virus constructs, which were considered to be equal to 1. The data represent the average of results from three independent experiments, performed in duplicate, with standard deviations.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

3T3-L1 adipocytic differentiation is associated with an increase in HMGI(Y) protein levels. We first investigated whether the expression of HMGI(Y) was regulated during adipocyte differentiation. As a model system, we used the 3T3-L1 preadipocytic cells, which have been extensively characterized. These cells undergo adipocytic conversion upon exposure to fetal bovine serum and differentiating agents (dexamethasone, methylisobutylxanthine, and insulin), as previously described (35). Cells were harvested in growing, undifferentiated conditions, at time zero (2 days postconfluence), and at different times during differentiation, and RNAs and proteins were prepared. Northern blot analysis showed that endogenous HMGI(Y) is expressed at low levels in growing cells, and it increases at time zero. It reaches its maximal level between 6 h and day 1 of treatment with differentiating agents and decreases again at day 4 (Fig. 1A), suggesting that the expression of HMGI(Y) is regulated, during differentiation, at the mRNA level. Western blot analysis showed a parallel increase of the HMGI(Y)-gene-specific protein product (Fig. 1B).

View larger version (24K): [in this window] [in a new window]   FIG. 1.   (A) HMGI(Y) induction during NIH 3T3-L1 preadipocyte differentiation. Total RNA (20 µg/lane) extracted from proliferating and differentiated 3T3-L1 cells was hybridized with the HMGI(Y) cDNA and then with a rat GAPDH probe as a control for RNA loading. RNA was extracted from undifferentiated proliferating cells (P) at time zero and at 6 h, 1 day, and 4 days of differentiation, as indicated. (B) Nuclear proteins extracted from normal and induced 3T3-L1 cells were separated (20 µg/lane) by SDS-PAGE and transferred to polyvinylidene difluoride membranes. Western blots were incubated first with antibodies specific for HMGI(Y) proteins and then with horseradish peroxidase-conjugated secondary antibodies; the immunocomplexes were detected by enhanced chemiluminescence. As a control for equal loading, the blotted proteins were stained with Ponceau Red. Moreover, the same Western blots were incubated with antibodies to the ubiquitous -tubulin protein. Proteins were extracted from the same cells as in panel A.

Inhibition of HMGI(Y) protein synthesis affects the differentiation and growth rate of 3T3-L1 cells. To investigate whether HMGI(Y) expression is a prerequisite for adipocytic differentiation, HMGI(Y) protein synthesis was suppressed by an antisense methodology. To this aim, 3T3-L1 cells were transfected with a plasmid carrying the HMGI(Y) gene in the antisense orientation (pCMV-HMGI-Yas) under the transcriptional control of the cytomegalovirus promoter (Fig. 2A). At the same time, to investigate the effect of the overexpression of HMGI(Y) in the 3T3-L1 cells, we generated the 3T3-L1-HMGI(Y)s cells, carrying the HMGI(Y) gene in the sense orientation [pCMV-HMGI(Y)s]. The empty vector (pCMVneo) served as a control. Six 3T3-L1-HMGI(Y)as clones showing the lowest HMGI(Y) protein levels, four overexpressing HMGI(Y), and one mass population for each transfection were chosen for further analyses. Cells transfected with the empty vector (four clones and a mass population) were used as a control for our experiments. A strand-specific RT-PCR assay showed the expression of the sense and antisense HMGI(Y) mRNA in the transfected cells (Fig. 2B and B'). The HMGI(Y) protein levels were remarkably reduced in the 3T3-L1-HMGI(Y)as cells, whereas they were increased in the 3T3-L1-HMGI(Y)s cells (Fig. 2C), compared with the parental and the backbone-vector-transfected cells. As a positive control for HMGI(Y) expression, we used the PC MPSV cell line (rat thyroid cells transformed by the myeloproliferative sarcoma virus), which expresses high levels of the protein (9). The HMGI(Y)as cells failed to undergo adipocytic differentiation upon being given differentiating treatment. Adipocyte differentiation of 3T3-L1 cells in culture is similar to the in vivo process, i.e., enlarged cells filled with lipid droplets and expressing adipocyte-specific gene products appear. As shown in Fig. 3A, the typical fat droplets did not appear in the cytosol of antisense-expressing cells following differentiating treatment. We also analyzed the expression of two adipocyte-specific molecular markers, the adipocyte lipid-binding protein, aP2, and the product of the obese gene, leptin. To this aim, we used a semiquantitative RT-PCR assay with parental, HMGI(Y)as, and pCMVneo 3T3-L1 cells. Upon differentiating treatment, induction of the aP2 and leptin genes was suppressed in 3T3-L1-HMGI(Y)as cells but not in the parental and the pCMVneo-transfected 3T3-L1 cells (Fig. 3B). When we analyzed the phenotype of the 3T3-L1-HMGI(Y)s cells, we did not observe any differences from the parental or the empty-vector-transfected 3T3-L1 cells (data not shown).

View larger version (25K): [in this window] [in a new window]   FIG. 2.   (A) HMGI(Y) sense and antisense constructs were generated as described in Materials and Methods. The untranslated region, DNA-binding domain, and acidic domain of the HMGI(Y) protein are indicated. (B) RT-PCR analysis of pHMGI(Y)s expression in 3T3-L1-HMGI(Y)s cells. The sources of RNAs are the following: lane 1, PCR on the pCMV-HMGI(Y)s plasmid (positive control); lanes 2, 3, and 4, 3T3-L1-HMGI(Y)s (cell clones 1, 2, and 3); lane 5, 3T3-L1 pCMVneo clone 1. (B') RT-PCR analysis of pHMGI(Y)as expression in 3T3-L1-HMGI(Y)as cells. The sources of RNAs are the following: lane 1, PCR on a pCMV-HMGI(Y)as plasmid (positive control); lanes 2, 3, and 4, 3T3-L1-HMGI(Y)as (cell clones 1, 2 and 3); lane 5, 3T3-L1 pCMVneo clone 1. All cDNAs were coamplified with GAPDH as an internal control. Bands of comparable intensity, obtained by the GAPDH sequence-specific primers, suggest comparable amplification of all samples. (C) 3T3-L1, 3T3-L1-HMGI(Y)as, 3T3-L1-HMGI(Y)s, and 3T3-L1 pCMVneo cell clones were treated with differentiating agents. Nuclear proteins were extracted and separated (20 µg/lane) by SDS-15% PAGE and transferred to polyvinylidene difluoride membranes. Western blots were incubated first with antibodies specific for the HMGI(Y) protein and then with horseradish peroxidase-conjugated secondary antibodies; the immunocomplexes were detected by enhanced chemiluminescence. As a control for equal loading, the blotted proteins were stained with Ponceau Red. The same Western blots were incubated with antibodies to the ubiquitous -tubulin protein. Sources of proteins were the following: lane 1, PC MPSV cells (positive control); lane 2, 3T3-L1 cells; lanes 3 and 4, 3T3-L1-HMGI(Y)as cells (clones 1 and 2); lane 5, 3T3-L1 pCMVneo clone 1; lanes 6, 7, and 8, 3T3-L1-HMGI(Y)s cells (clones 1, 2, and 3).

View larger version (64K): [in this window] [in a new window]   FIG. 3.   (A) Inhibition of adipocytic differentiation induced by blockage of HMGI(Y) synthesis. Adipogenic differentiation of normal and pCMVneo- or pCMV-HMGI(Y)as-transfected 3T3-L1 cells is shown. Cell clones were cultured in the presence of standard differentiation induction medium containing 0.5 mM 1-methyl-3-isobutylxanthine, 1 mM dexamethasone, 5 µg of insulin/ml, and 10% fetal bovine serum. After 8 days of differentiation, cells were observed by light microscopy. Magnification, ×400. This experiment is representative of five independent assays. (B) mRNA levels of aP2 and leptin were determined by RT-PCR, gel electrophoresis, and Southern blot hybridization. For details, see Materials and Methods. The cDNAs were coamplified with GAPDH, as an internal control. No bands are seen in non-reverse-transcribed RNAs, thus excluding DNA contamination (data not shown). RNAs were extracted from these cells at days 0, 4, and 7 of differentiation, as indicated.

View larger version (11K): [in this window] [in a new window]   FIG. 4.   (A) Cells were grown as described in Materials and Methods and counted daily. (B) Cells were grown until confluence and then induced to differentiate as described in Materials and Methods. Cell counts started 1 day after the addition of the differentiation cocktail, as indicated, and were performed daily. For each type of experiment, one representative clone is shown; the results were confirmed on two additional clones. The data reported are the average results of two independent experiments.

HMGI(Y) physically interacts with C/EBP proteins. Transcriptional regulation of adipocyte differentiation requires the concerted activity of several transcription factors that control growth arrest and the coordinated expression of adipocyte-specific genes. Among these transcription factors, C/EBP proteins play a critical role in the development of the adipocyte differentiation program. Indeed, the levels of the C/EBP proteins increase during adipocyte differentiation. The increase of C/EBP and C/EBP occurs early during differentiation and is followed by the increase of C/EBP, which ultimately controls the expression of several genes, among which are the genes for aP2 and leptin (26). Our data indicated that HMGI(Y) proteins are also required for differentiation of 3T3-L1 preadipocytes. These observations suggested that HMGI(Y) might influence adipocytic differentiation through interactions with the C/EBP transcription factors. To test this hypothesis, 3T3-L1 cells were synchronously differentiated into adipocytes by hormonal treatment. Cells were harvested at time zero and at various times during differentiation, and the interaction between C/EBP and HMGI(Y) was examined by coimmunoprecipitation experiments. Protein extracts were immunoprecipitated with anti-C/EBP antisera and immunoblotted with anti-HMGI(Y) antibodies (Fig. 5A, upper panel). Interaction between C/EBP and HMGI(Y) was detected at time zero; it increased at 6 h and remained stable until day 6 of differentiation. This interaction was not detected when a preimmune serum was used for lysate extracts of 6 h (Fig. 5A). In agreement with previous observations, C/EBP levels increased during early differentiation of 3T3-L1 cells (Fig. 5A, lower panel). We also detected binding of HMGI(Y) and the other two C/EBP proteins (not shown); these interactions occurred with different kinetics.

View larger version (23K): [in this window] [in a new window]   FIG. 5.   (A) Interaction between C/EBP and HMGI(Y). Cell lysates were prepared from 3T3-L1 cells at the indicated times as described in Materials and Methods. Proteins were immunoprecipitated with antibodies directed against the protein C/EBP (Santa Cruz Biotechnology), as indicated. Immunoprecipitated (I.P.) proteins were immunoblotted with anti-HMGI(Y). Levels of C/EBP during differentiation are shown. IgG, immunoglobulin G. (B) Wild-type C/EBP protein was in vitro translated as described in Materials and Methods. Rabbit reticulocyte extracts were mixed with GST-HMG-Y recombinant protein for binding assays. Binding reaction products were washed, and proteins were separated on a polyacrylamide gel. Filters were probed with the anti-C/EBP antibody (Santa Cruz Biotechnology). (C) C/EBP and HMGI(Y) interaction in 293 cells. 293 cells were transfected with the indicated expression plasmids as described in Materials and Methods. Cell lysates were prepared, and equal amounts of proteins were immunoprecipitated with the indicated antibodies. In the top two panels, lane 1 shows a control immunoprecipitation. In lanes 2 and 3, the indicated cell lysates were immunoprecipitated either with the anti-C/EBP antibody (upper panel) or with the anti-HMGI(Y) antibody. The immunocomplexes were immunoblotted with the reciprocal antibodies, as indicated. In the bottom two panels, Western blot analysis shows the amounts of C/EBP and HMGI(Y) for each lysate used in the top two panels.

Mapping of the HMGI(Y) region responsible for binding to C/EBP. To map the HMGI(Y) region required for binding to C/EBP, we generated a series of progressive deletions of the HMGI(Y) gene in an area corresponding to the carboxy-terminal region of its product (Fig. 6A). The resulting cDNAs were tagged with the influenza virus HA epitope and cloned into the pCEFL expression vector. Immunoblotting analysis showed that approximately equal amounts of wild-type and mutant proteins were produced. These mutants were tested for their interaction in vivo with C/EBP in coimmunoprecipitation experiments. Each HMGI(Y) plasmid was transfected in 293 cells together with a C/EBP-expressing vector. Thirty-six hours after transfection, cells were harvested and protein extracts were immunoprecipitated with anti-C/EBP antibodies. As shown in Fig. 6B, deletion of the carboxy-terminal tail and of the third basic repeat did not impair the binding of the HMGI(Y) protein to C/EBP [compare wild-type HMGI(Y) with mutant 1-63]. Conversely, removal of the region between the middle and the last basic repeat (amino acids 54 to 63) and of the second repeat is detrimental to the interaction of HMGI(Y) with C/EBP [compare wild-type HMGI(Y) with mutants 1-53 and 1-43]. These results demonstrate that the carboxy-terminal tail and the third basic repeat are not essential for this interaction, whereas the region between the second and third repeats is required for the binding of HMGI(Y) and C/EBP.

View larger version (23K): [in this window] [in a new window]   FIG. 6.   The region between the second and the third AT hook is required for HMGI(Y)-C/EBP interaction. (A) Schematic diagram of plasmids expressing HA-tagged wild-type HMGI(Y), showing the 1- 63, 1-53, and 1-43 deletion mutant proteins. The HA epitope tag, AT hooks (+), and C-terminal (----) domains are also indicated. (B) 293 cells were transiently cotransfected with C/EBP and the indicated HMGI(Y) mutant plasmids. Equal amounts of cell lysates (2 mg) were immunoprecipitated with anti-C/EBP antibodies, and the immunocomplexes were probed with either anti-C/EBP (upper panel) or anti-HA antibodies (lower panel). Aliquots of the same lysates (50 µg) were probed with anti-HA antibodies to evaluate the comparable expression of the transfected plasmids.

HMGI(Y) cooperates with C/EBP in the regulation of the obese gene promoter, a C/EBP-regulated gene. The foregoing results suggested that C/EBP cooperates with HMGI(Y) in the activation and/or repression of adipocyte-specific gene promoters. We focused on the promoter of the leptin protein, encoded by the obese gene, since its expression during adipocytic induction seems to depend on HMGI(Y) synthesis. We first used the obese (ob) minimal promoter (161), which contains C/EBP motifs and which is a natural target of C/EBP transcription factors (20, 28). A plasmid containing the ob minimal promoter fused to the luciferase reporter gene (161 ob-luc) was transfected in 293 cells with or without C/EBP. As shown in Fig. 7A, C/EBP activated luciferase transcription. When C/EBP was cotransfected with HMGI(Y), activation of the ob promoter was significantly potentiated. Conversely, no activation was observed in the presence of HMGI(Y) alone or when the m52-ob-luc promoter, which is mutated in the C/EBP-binding site, was used for the cooperativity assay. Analogous results were obtained when we used the ob-luc-762 long promoter (data not shown). As a further control for the specificity of the stimulatory effect of HMGI(Y) on C/EBP-mediated transactivating activity, we used another reporter vector, RSV-luc. This vector contains a promoter which has a low basal activity in 293 cells and is insensitive to C/EBP. As shown in Fig. 7B, neither C/EBP or HMGI(Y) alone nor the combination of the two proteins was able to significantly stimulate this promoter. Moreover, we have demonstrated that HMGI(Y) is also able to cooperate with C/EBP in the transactivation of the leptin promoter (data not shown).

View larger version (36K): [in this window] [in a new window]   FIG. 7.   (A) Leptin transactivation by C/EBP and cooperation with HMGI(Y). Histograms show the luciferase activities of extracts from 293 cells cotransfected with the p(161)ob-luc reporter and the indicated C/EBP and HMGI(Y) plasmids. The mutant m52 reporter plasmid was used as a negative control. (B) Histograms showing the luciferase activities of extracts from 293 cells cotransfected with the RSV-luc reporter and the C/EBP and HMGI(Y) plasmids. The relative activities were calculated by dividing the normalized activities by the activity of the m52 and RSV-luc constructs, which has been considered equal to 1. The data represent the average of results of three independent experiments, performed in duplicate, with standard deviations. (C) After transfection, cell lysates were divided into two aliquots. One of these aliquots was used for transactivation assays, and the other was used for Western blot analysis as a control of protein expression. Protein extracts were separated by SDS-PAGE, transferred to Immobilon-P, and immunoblotted with the indicated antibodies.

	DISCUSSION

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Adipocytic differentiation requires HMGI(Y). HMGI(Y) and HMGI-C proteins are important architectural transcription factors (19), and a growing body of evidence suggests that they are involved in adipocytic differentiation (2, 34, 40, 49). We have investigated the role of HMGI(Y) proteins in adipogenesis using 3T3-L1 preadipocytic cells as a model system. Northern and Western blot analyses demonstrated induction of the HMGI(Y) gene and protein when 3T3-L1 cells were induced to differentiate into adipocytes. HMGI(Y) expression is detectable at very low levels in growing, undifferentiated 3T3-L1 cells and increases during differentiation. These observations suggested that an increase in HMGI(Y) levels is necessary for 3T3-L1 differentiation. Indeed, suppression of HMGI(Y) protein synthesis through antisense methodology prevented terminal adipocytic differentiation. Not only did these cells not show the typical fat-laden phenotype, but they also lacked the expression of two adipocytic markers, aP2 and leptin. On the other hand, forced expression of the HMGI(Y) gene resulted in inhibition of growth, but it was not able to induce differentiation. These data demonstrated that the wild-type HMGI(Y) protein is required, but not sufficient, for 3T3-L1 cells to differentiate into adipocytes. Three members of the C/EBP family of transcription factors (C/EBP, -, and -) have been implicated in the induction of adipocyte differentiation. In particular, overexpression of C/EBP is sufficient to arrest growth and to start the adipocyte differentiation program in preadipocytic cell lines (26). Recent data obtained in our laboratory indicate that its forced expression in the 3T3-L1 HMGI(Y)as cells is not able to revert their phenotype, i.e., the block of growth arrest and adipocytic differentiation (data not shown). These data indicate that HMGI(Y) is indeed necessary for C/EBP to induce its biological effects. This hypothesis is also supported by results of other experiments. The promoters of several adipocyte-specific genes contain C/EBP regulatory binding sites. For instance, C/EBP was shown to bind and transactivate the aP2 promoter. Furthermore, the leptin promoter contains at least one functional C/EBP binding site: disruption of this consensus sequence by site-directed mutagenesis causes a remarkable decrease in promoter activity (20, 28). Based on these observations, we argued that HMGI(Y) modulates the transcriptional activity of the C/EBPs. This hypothesis was confirmed by the finding that HMGI(Y) physically interacts with C/EBP transcription factors.

HMGI(Y) proteins suppress 3T3-L1 cell proliferation. In 3T3-L1 cells undergoing adipocyte differentiation, there is a G₁/G₀ arrest at confluence, followed by a phase of clonal expansion initiated by the differentiating agents (26) and a subsequent arrest about 2 days later. We show that suppression of HMGI(Y) expression causes a blockage in the differentiation associated with an increased growth rate in 3T3-L1 cells. Furthermore, treatment of HMGI(Y) antisense-expressing cells with differentiating agents failed to induce the cell cycle arrest that precedes differentiation. Therefore, we suggest that HMGI(Y) plays a critical role in adipocytic cell growth. The levels of HMGI(Y) do not correlate with the cell cycle status of the cells during induction of differentiation, being highest in the phase of mitotic clonal expansion (6 h and day 1) and reduced in growth-arrested, differentiating cells (days 0 and 4). This paradox could be explained by the fact that HMGI(Y) is an accessory protein, with multiple functions, for a wide range of transcription factors. Its effect could enhance growth arrest or proliferation depending on the presence of different transcription factors.

HMGI(Y) proteins physically interact with C/EBP and modulate C/EBP-mediated transcription. C/EBPs play a pivotal role in adipogenesis (6, 37). Here we demonstrate that HMGI(Y) binds to C/EBP in 3T3-L1 cells and in 293 cells by reciprocal coimmunoprecipitation. We also show that a GST-HMGI(Y) fusion protein binds C/EBP in a typical pull-down assay. Furthermore, we demonstrate that this binding requires the region between amino acids 53 and 63 of HMGI(Y). By binding to C/EBPs, HMGI(Y) may functionally cooperate with these proteins to activate and/or repress different promoters. Preliminary results obtained by using band shift analysis with a C/EBP-specific oligonucleotide show that high-molecular-weight complexes are formed at different times during 3T3-L1 differentiation (G. M. Pierantoni and R. M. Melillo, personal communication): HMGI(Y) may be involved in the assembly of higher-order complexes that are essential for both arrest of growth and the expression of the differentiated phenotype of adipocyte precursors. Consistent with the idea that HMGI(Y) serves as a general cofactor of adipocyte-specific transcription, we observed that HMGI(Y) expression modulates the transcription of a gene whose levels are regulated during adipocyte differentiation: the obese gene coding for leptin (48). Both C/EBP and C/EBP bind to the C/EBP consensus site on the leptin promoter and are able to activate transcription of the obese gene (28). Here we show that HMGI(Y) functions as a specific cofactor for C/EBP. Indeed, we demonstrate that C/EBP-mediated activation of the leptin promoter was strongly potentiated by the presence of the HMGI(Y) protein. The same results were obtained with the C/EBP transcription factor, suggesting that HMGI(Y) is an accessory factor for this family of proteins. This is consistent with the observation that HMGI(Y) is also able to bind to C/EBP and - (data not shown). Analysis of the obese minimal promoter sequence failed to identify putative HMGI(Y)-DNA binding sites, which are represented by tracts of adenines and thymines arranged on the same face of the DNA helix. Furthermore, electrophoretic mobility shift analysis performed in vitro with purified recombinant HMGI(Y) and the ob minimal promoter showed no high-affinity HMGI(Y) DNA-binding sites (Pierantoni and Melillo, personal communication). These observations, together with the ability of HMGI(Y) to bind C/EBP in solution in the absence of DNA, argue against the presence of HMGI(Y) in C/EBP-DNA complexes: it is possible that HMGI(Y) facilitates the binding of C/EBP to DNA by transiently associating with C/EBP.

Conclusions. The data presented here show that HMGI(Y) exerts a negative effect on the proliferation of adipocyte precursors and a positive effect on differentiation. This dual role is consistent with the finding that HMGI proteins may positively and negatively affect gene expression. We also demonstrate that the HMGI(Y) protein physically interacts with C/EBP proteins and that it functionally cooperates in the transcriptional activity mediated by these proteins, whose function is required to trigger the expression of adipocyte-specific genes.