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Molecular and Cellular Biology, October 2002, p. 7351-7363, Vol. 22, No. 20
0270-7306/02/$04.00+0 DOI: 10.1128/MCB.22.20.7351-7363.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Laura Coda,1 Antonio De Giuseppe,3 Massimo Santoro,2 and Pier Giuseppe Pelicci1,4,5*
Department of Experimental Oncology, European Institute of Oncology, 20141 Milan,1 Dipartimento di Biologia e Patologia Cellulare e Molecolare, Centro di Endocrinologia ed Oncologia Sperimentale del CNR, 80131 Naples,2 Istituto di Medicina Interna e Scienze Oncologiche, Perugia University, 06100 Perugia,3 FIRC Institute for Molecular Oncology (IFOM), 20139 Milan,4 Università Vita-Salute San Rassaele, Milan, Italy5
Received 26 December 2001/ Returned for modification 8 February 2002/ Accepted 22 July 2002
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ABSTRACT |
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 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
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INTRODUCTION |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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The modular organization of the proteins of the Shc family suggests that they function as adaptors within cytoplasmic signaling pathways. Indeed, several lines of evidence support a general role for the p52/46Shc proteins in the transduction of signals from activated tyrosine kinases (TKs) to Ras. p52/46Shc proteins are rapidly and efficiently tyrosine-phosphorylated by all TKs tested to date (2). Three major Shc phosphorylation sites have been identified within its CH1 region (31, 39). Once phosphorylated, the p52/46Shc proteins act to bridge receptor TKs (RTKs) and the Grb2 adaptor protein (2). Grb2, in turn, is constitutively complexed to SOS, a Ras guanine nucleotide exchange factor (4). Recruitment of the Grb2/SOS complex by p52/46Shc results in the membrane relocalization of SOS, an event sufficient to induce Ras activation. Consistent with this model, overexpression of p52/p46Shc enhances epidermal growth factor (EGF)- or granulocyte-macrophage colony-stimulating factor-induced Ras downstream signaling events (2), such as mitogen-activated protein kinase (MAPK) and fos promoter activation. Moreover, fibroblasts derived from Shc-null mice display a reduced MAPK activation in response to physiological concentrations of growth factors (16). In line with their role in the activation of Ras, the p52/p46Shc proteins are ubiquitously expressed in the adult mice.
Recent results have suggested that the Shc proteins could have further, apparently unrelated, functions in addition to Ras activation. Homozygous inactivation of p66Shc in mice extends their life spans and increases cellular resistance to oxidative stress-induced apoptosis (21). p66Shc is not involved in Ras activation (22). Instead, it is implicated in pathways activated by environmental stresses, as shown by its serine phosphorylation in cells exposed to oxidative stress (21).
Unlike Shc, which is variably expressed in many different tissues, expression of the other two Shc-related genes is either exclusively (Rai) or predominantly (Sli) restricted to the nervous system (25-27). Null mutations of Sli and Rai in mice resulted in no overt neuroanatomical abnormalities within the central nervous system. However, analysis of sympathetic and sensory neuronal populations revealed loss of sympathetic neurons in the mice lacking both Sli and Rai (but not in the single-null mutants) as well as loss of sensory neurons (associated with nociceptive function) in the Sli-deficient mice (30). These findings suggest that Rai and Sli regulate neural development and that they perform redundant functions in supporting sympathetic neuron development and survival while Sli plays a nonredundant role in the survival of sensory neurons. However, the biochemical mechanisms underlying the neural functions of Sli and Rai remain largely unknown.
Neurons of the developing sympathetic superior cervical ganglia (SCG) express high levels of both Rai and Sli transcripts and are significantly reduced in number in mice carrying double mutations of Sli and Rai (30). Loss of either the TrkA (9, 36) or the Ret (34) receptor, but not of the TrkB or TrkC receptors (9), results in virtual elimination of SCG neurons, thereby suggesting that the Rai/Sli mutant phenotype may be caused by the disruption of TrkA and/or Ret signaling. Indeed, preliminary evidences suggest that, like the Shc proteins, Sli and Rai may function as cytoplasmic signaling proteins downstream of activated TK receptors. Both Rai and Sli can bind activated TK receptors, including the EGF receptor (EGFR) and Trk receptor (10, 23, 24, 26). Whether Rai or Sli activates Ras or other intracellular pathways remains to be determined, as do the biological consequences of their activities.
Here, we report that Rai is a physiological substrate of the Ret receptor and that it exerts a prosurvival function in neuronal cells by activating the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway.
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MATERIALS AND METHODS |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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N1059, or the Y905F mutation were previously described (11, 20, 32). The chimeric EGFR/Ret (E/R) receptor was reported previously (33). Tyrosine-to-phenylalanine mutations were introduced in the p52Rai and Ret-MEN2A cDNAs by in vitro site-directed mutagenesis. The bovine p85 (into pcDNA3.1) cDNA was a gift of R. Hooshmand-Raad. To generate the Shc(CH1Rai) and Rai(CH1Shc) constructs, the Shc and Rai CH1 regions were amplified by PCR, sequenced, and inserted in frame into the corresponding location of hemagglutinin (HA)-Rai and HA-Shc, respectively.
Cell culture, infection, and transfection.
SK-N-MC E/R and PC12coR
cells were obtained by stably transfecting SK-N-MC or PC12 cells with E/R and GFR1 (gift from S. Jing) expression vectors, respectively. Transfection of the Phoenix packaging cell line and infection of target cells were performed as described previously (12). The efficiency of infection, measured as GFP positivity, was evaluated by fluorescence-activated cell sorter (FACS) analysis, and it was consistently stable over months and >95%. Transfections of SK-N-BE(2) cells were performed using Lipofectamine (Gibco BRL).
Differentiation of PC12coR cells and apoptosis detection.
PC12coR cells were plated onto 60-mm-diameter polylysine-coated dishes in 1% horse serum at a density of 10,000 cells/dish in the absence or presence of glial cell line-derived neurotrophic factor (GDNF) (1 to 100 ng/ml). Every second day, the cells were examined under light microscopy and fresh medium and growth factors were added.
Neurite outgrowth was quantitated by scoring the number of cells with neuritis longer than the size of two cell bodies. Results were expressed as increases (n-fold) in percentage with respect to unstimulated control cells. The statistical analysis was performed on the basis of the results of three experiments, each of them done in triplicate.
Apoptosis was measured by FACS analysis of propidium iodide-stained cells.
Immunoprecipitation, in vitro binding assay, and Far Western experiments. Cell cultures were serum starved for 24 h and treated with EGF (100 ng/ml), GDNF (1 to 100 ng/ml), nerve growth factor (NGF) (50 ng/ml), or neurturin (NTN) (100 ng/ml). The cells were then lysed at 4°C in ice-cold lysis buffer (27, 33). For immunoprecipitations, clarified lysates were incubated with polyclonal antibodies against p85, Ret, the CH1 regions of Rai and Shc, or the monoclonal anti-EGFR antibody (27, 33). For in vitro binding assays, total protein lysates (1 mg) were incubated for 2 h at 4°C with 10 µg of the appropriate GST fusion protein. For Far Western experiments, blots were blocked in 2% bovine serum albumin (BSA) in TTBS (20 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.05% Tween 20) for 2 h at room temperature and then incubated with reduced glutathione (3 µM) in TTBS with 0.5% BSA (wt/vol) for 1 h at room temperature. Blots were then incubated with the GST p85/N-SH2 or GST p85/C-SH2 proteins (10 nM) in TTBS in the presence of reduced glutathione and BSA. After extensive washing in TTBS, blots were detected with the affinity-purified anti-GST antibody.
SDS-PAGE and Western blotting. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting were performed according to established procedures. The following monoclonal antibodies were used: anti-EGFR (33), anti-pTyr (Upstate Biotechnology), anti-vinculin (Sigma), and anti-ErkP (Cell Signaling). The polyclonal antibodies anti-CH1Rai, anti-SH2Rai (27), anti-CH1Shc (27), anti-p85 (Upstate Biotechnology), anti-Ret (32), anti-AktP (Ser437; New England Biolabs), anti-Akt (New England Biolabs), anti-Erks (Santa Cruz Biotechnology), and anti-Grb2(Santa Cruz Biotechnology) were also used. Densitometry measurements of both total and phosphoprotein-specific Erks immunoblots were performed by PhosphorImager analysis (Typhoon 8600; Molecular Dynamics).
In vitro PI3K assay.
Lysate (300 µg) was immunoprecipitated with anti-p85 antibodies. The kinase reaction was performed for 30 min at room temperature in the PI3K buffer (20 mM Tris-HCl [pH 7.5], 100 mM NaCl, 0.5 mM EGTA [pH 8], 25 mM MgCl2, 10 µCi of [
-32P]ATP, 1 µg of phosphatidylinositol) and terminated by the addition of HCl. Thin-layer chromatography was conducted on Whatman silica gel plates in a closed thin-layer chromatography tank with methanol-chloroform-water-ammonia (47:60:11.3:2 [vol/vol/vol/vol]) as a buffer. The plates were processed for autoradiography, and the level of incorporation of radioactive ATP was quantitated with a phosphorimager.
In vitro kinase assay.
Ret proteins were immunoprecipitated from Cos cells transiently transfected with Ret/MEN2A or RETY905F constructs. The immunoprecipitated Ret proteins were suspended in a kinase buffer (40 mM HEPES-KOH [pH 8], 40 mM potassium glutamate, 1 mM EGTA, 0.5 mM EDTA, 8 mM magnesium acetate, 2 mM dithiothreitol, 10 mM sodium fluoride) with radiolabeled [-32P]ATP and 3 µg of MBP (Sigma Chemical Co.) or immunoprecipitated Rai proteins derived from lysates of Cos cells transiently transfected with Rai or RaiFFF cDNAs. The kinase reaction was carried out for 25 min in a 30°C water bath and was terminated by adding SDS sample buffer with 2-mercaptoethanol. The proteins were resolved on 12% denaturing gels.
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RESULTS |
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Similar experiments were performed in SK-N-BE(2) cells engineered to express the NGF receptor (TrkA). As in the case of EGF, NGF treatment failed to induce detectable tyrosine phosphorylation of the Rai proteins (Fig. 1D). Notably, EGF, GDNF, or NGF induced tyrosine phosphorylation of the cognate Shc proteins in the same cells (under identical experimental conditions) (Fig. 1D). It appears, therefore, that Rai, at variance with Shc, is not a common substrate of receptor TKs and that, of the analyzed receptors, it is activated specifically by Ret. We cannot exclude, however, that Rai functions as an adaptor protein for other activated receptors. Although we were unable to demonstrate phosphorylation of endogenous Rai proteins following EGF or NGF treatment of neural cells, we observed Rai phosphorylation in Cos cells transiently transfected with Rai and treated with pharmacological (100 ng/ml) doses of EGF (unpublished results) or cotransfected with Rai and TrkA and treated with NGF (unpublished results). Similarly, phosphorylation of overexpressed Rai has been recently reported in PC12 cells treated with NGF (23). Therefore, it is possible that, under specific physiological conditions, Rai may function as a substrate of activated growth factor receptors other than Ret.
Rai expression enhances the effects of activated Ret on survival and differentiation of PC12 cells.
To investigate its function in the Ret signaling pathway, Rai was ectopically expressed in PC12coR cells. PC12 is a well-established model system for studying differentiation and survival in a neuronal cell setting. Furthermore, PC12 cells do not express Rai (Fig. 1B), thereby allowing analysis of the effects of its ectopic expression. Although parental PC12 cells express Ret, they respond poorly to GDNF, due to the absence of GFR1 (coR), a glycosyl phosphatidylinositol-linked GDNF receptor that is necessary for the activation of Ret by GDNF (15). Stable expression of GFR1 in PC12 cells (PC12coR cells) rendered cells responsive to GDNF, as revealed by Ret autophosphorylation following GDNF (data not shown). We selected Rai-transduced PC12coR clones with levels of overexpressed protein comparable to those of endogenous Rai of SK-N-BE(2) cells or adult mouse brain (Fig. 2A).
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cells led to a loss of cell viability after 48 h, due to induction of apoptosis (Fig. 2B). High doses (100 ng/ml) of GDNF partially reversed this response, leading to the survival of serum-starved cells (Fig. 2B) and allowing their terminal differentiation (Fig. 2C). In contrast, treatment with low doses (1 ng/ml) of GDNF had only a marginal effect on survival (Fig. 2B) and differentiation (Fig. 2C and D) of PC12coR cells. Notably, the expression of Rai-sensitized PC12coR cells had effects similar to those of low doses (1 ng/ml) of GDNF on both survival (Fig. 2B) and differentiation (Fig. 2C and D). Intermediate doses of GDNF (10 ng/ml) almost completely suppressed serum starvation-induced apoptosis of PC12coR cells (Fig. 2B) but failed to induce terminal differentiation (Fig. 2C and D), in the absence of Rai. In contrast, neurite outgrowth was easily detected in the PC12coR cells expressing Rai following intermediate doses of GDNF (Fig. 2C and D).
These results suggest that Rai is a functional substrate of Ret and that it enhances the survival and differentiable effects of Ret in neuronal cells. Interestingly, we noticed that survival of serum-starved PC12coR cells expressing Rai was slightly, yet consistently, higher than that of control cells (Fig. 2B), suggesting that Rai exerts a slightly inhibitory effect on apoptosis of PC12 cells even in the absence of GDNF treatment and Ret activation. This putative function of Rai in established neuronal cells parallels those previously reported in central nervous system progenitor cells obtained from embryonic-day-14 rat striatum and grown in a minimal medium (5).
Rai does not bind Grb2 and increases late (GDNF-induced) MAPK activation. To investigate the molecular mechanisms underlying the effects of Rai on survival and differentiation, we first analyzed its ability to activate the Ras signaling pathway by measuring the extent and the kinetics of MAPK activation in Shc- or Rai-overexpressing cells following GDNF or EGF treatment. In the GDNF-treated control SK-N-BE(2) cells, phosphorylated p44 and p42 Erk2 were detected at 5 and 15 min following treatment while they were almost undetectable after 30 min (Fig. 3A and B). Under the same experimental conditions, Shc overexpression induced increased MAPK activation at both early (5 and 15 min) and later (30 min) time points. Rai overexpression, instead, had no effects on the early peak (5 and 15 min) of MAPK activation, whereas it increased activation at later time points (30 min) (Fig. 3A and B). No effect of Rai overexpression was, instead, observed on EGF-induced MAPK activation (Fig. 3C and D).
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coreceptor and confines the analysis to Ret-mediated signaling. Indeed, the C634R MEN2A mutation induces Ret activation through disulfide-linked receptor dimerization (32). Protein lysates were immunoprecipitated with specific anti-Rai antibodies and analyzed by anti-phosphotyrosine blots. Rai tyrosine phosphorylation decreased in the FYY and FFY mutants and almost disappeared in the FFF mutant (Fig. 4A). Similar results were obtained in SK-N-MC cells expressing the chimeric E/R receptor and transfected with the parental or the phosphorylation-defective FFF Rai cDNAs (Fig. 4B). In these cells, the extracellular domains of the EGFR are fused to the cytoplasmic portion of Ret, thereby allowing the activation of the Ret signaling pathway by EGF (33). Furthermore, the use of this artificial construct allows analysis of Ret independent of autonomous Ret signals initiated by GFR1, when cells are triggered by GDNF (38). EGF stimulation induced tyrosine phosphorylation of the wild-type, but not of the FFF, Rai protein (Fig. 4B), thereby suggesting that, as for the Shc proteins, Y231, Y232, and Y304 are the major Rai tyrosine phosphorylation sites. These results differ from those of a previous report, which showed that NGF treatment induces phosphorylation of overexpressed Rai only at the Y304 site, possibly due to differences in the experimental systems used (GDNF versus NGF and endogenous versus overexpressed Rai proteins) (23).
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Rai expression increases PI3K activation and Akt phosphorylation by activated Ret.
Activated RTKs promote membrane recruitment of PI3K, which then leads to the local formation of PI(3,4)P2 and PI(3,4,5)P3 (17). Akt is a kinase which, upon binding to PI(3,4)P2 and PI(3,4,5)P3, is phosphorylated by other serine/threonine kinases (PDK1 and PDK2) and, in turn, phosphorylates several substrates involved in the suppression of apoptosis (7). Thus, to investigate further the mechanisms of the anti-apoptotic function of Rai, we analyzed its effects on the Ret-mediated activation of the PI3K/Akt pathway by using three different cell systems: PC12 cells, PC12CoR cells, and SK-N-MC neuronal precursors expressing an E/R chimera. We evaluated the effects of Rai on Akt activation by measuring the status of Akt phosphorylation with anti-phospho-Akt antibodies. Following Ret stimulation, ectopic expression of Rai protein induced Akt activation in PC12 cells (Fig. 5A). In PC12CoR cells, Akt phosphorylation rapidly declined after 15 min of GDNF stimulation, and it remained elevated for 120 min in Rai-expressing cells (Fig. 5B). In SK-N-MC cells expressing the E/R chimera, Akt phosphorylation was potentiated by Rai expression (Fig. 5C). Interestingly, also in the absence of added growth factors, Rai expression slightly increased basal phospho-Akt levels in PC12, PC12CoR, and SK-N-MC E/R cells (Fig. 5A to C).
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Binding of Rai to the p85 subunit of PI3K. To study the mechanism(s) underlying the effects of Rai on PI3K, we first investigated whether Rai forms a stable complex with PI3K in vivo by using cells that had been transiently transfected with cDNAs encoding Rai, Ret-MEN2A, and the p85 subunit of PI3K. Anti-p85 Western blots of anti-Rai immunoprecipitates revealed the existence of a p85-Rai complex in both the presence and the absence of cotransfected Ret-MEN2A (Fig. 6A, upper panel). However, anti-phosphotyrosine blots of the same anti-Rai immunoprecipitates revealed the presence of phosphorylated Rai proteins only when Ret-MEN2A was cotransfected (Fig. 6A, middle panel). Similar experiments performed with SK-N-MC E/R cells revealed the existence of a stable Rai-p85 complex (Fig. 6B, upper panel) both in SF conditions (where Rai is hypophosphorylated) and following Ret activation by EGF (where Rai is hyperphosphorylated) (Fig. 6B, middle panel). Though these experiments were performed with overexpressed proteins, the results suggest that the phosphorylated and hypophosphorylated Rai proteins form a constitutive complex with p85 in vivo.
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To investigate the biological role of Rai-tyrosine phosphorylation, we analyzed the capacity of the phosphorylation-defective Rai FFF mutant to stimulate PI3K activity, Akt phosphorylation, and survival of neuronal cells. In the absence of Ret activation (that is, under SF conditions), the Rai FFF mutant retained the capacity of the wild-type protein to activate PI3K (Fig. 5D) and Akt (Fig. 5C), to promote survival (Fig. 5E), and to interact with p85 (Fig. 4B). In contrast, following Ret stimulation, the levels of activation of PI3K (Fig. 5D) and Akt (Fig. 5C) were comparable in the cells not expressing Rai (controls) and in those expressing the Rai FFF mutant. It appears, therefore, that the effects of Rai expression on the PI3K/Akt pathway and on survival are based on two different mechanisms, one which is dependent on Ret activation and Rai phosphorylation and another which does not require Ret or Rai phosphorylation. In both cases, however, a stable Rai-p85 complex is formed.
Phosphorylation of the Ret-MEN2A tyrosine Y1062 residue is indispensable for recruitment and phosphorylation of Rai and for PI3K/Akt activation.
Mutation of the Ret-MEN2A Tyr 1062 residue (Y1062F) eliminates binding of Ret to p85 as well as Ret-mediated activation of PI3K and Akt (8, 35). Similarly, deletion of an asparagine residue close to Y1062 (N1059) impairs Shc binding to Ret and the subsequent activation of downstream signaling pathways (11). Notably, asparagine 1059 has been found to be missing in patients affected by congenital megacolon or Hirschsprung's disease, a defect of development of the enteric nervous system which is caused by loss of function of the Ret receptor (11).
To explore the mechanisms through which Rai potentiates the effects of Ret on PI3K, we investigated whether Y1062 is involved in Rai phosphorylation and recruitment. The cDNAs for p52Rai and Ret-MEN2A (or its mutants) were transiently cotransfected into Cos cells, and the extent of Rai phosphorylation and Ret-Rai interaction was analyzed. Probing of anti-Ret immunoprecipitates with an anti-Rai antibody revealed the existence of a stable complex between the wild-type Ret-MEN2A protein and Rai (Fig. 7A, upper panel). Anti-phosphotyrosine staining of the same immunoprecipitates showed that the Ret-bound Rai polypeptides are phosphorylated (Fig. 7A, middle panel). Both the Y1062F and N1059 mutations markedly reduced the degree of Rai phosphorylation and association with Ret-MEN2A, indicating that the Ret-MEN2A tyrosine 1062 residue is a docking site for Rai. This was confirmed by in vitro binding experiments, which showed the following. (i) A GST fusion protein of the cytoplasmic tail of Ret containing Y1062 (Ret-COOH) binds Rai in a pull-down experiment, and such binding is dependent on GST-Ret-COOH tyrosine phosphorylation (Fig. 7B). (ii) Rai binds Ret through its PTB and (to a lesser extent) SH2 domains, and the Y1062F Ret mutation prevents this binding (Fig. 7C). (iii) Progressively increasing amounts of a Ret peptide encompassing the phosphorylated tyrosine 1062 (pp1062) (but not the corresponding dephosphorylated peptide, p1062) compete with binding of the Rai-PTB domain to Ret (Fig. 7D). Together, these results suggest a model in which phosphorylated Rai functions as an adaptor molecule for the recruitment of PI3K to phosphorylated Ret tyrosine 1062.
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DISCUSSION |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Mice lacking both Rai and Sli, but neither mutant alone, exhibit a significant loss of neurons within the SCG. Notably, the number of sympathetic SCG neurons in the double-mutant mice was reduced at birth and by 8 weeks postnatal, but it had been normal at embryonic day 13.5, which suggests a loss of postmitotic neurons during the later phase of embryonic development rather than a defect in neurogenesis (30). Since mutations of either TrkA or Ret result in a virtual elimination of SCG neurons, it has been suggested that Rai and Sli possess functionally redundant roles in the cytoplasmic propagation of survival signals from activated TrkA and/or Ret in SCG neurons (30). Here, we have demonstrated that in cultured neural cells, Rai is a physiological substrate of Ret and that its expression potentiates the survival effects of activated Ret. Therefore, one of the physiological functions of Rai appears to be that of transducing Ret-dependent survival signals in a subpopulation of sympathetic neurons. We cannot exclude, however, that Rai functions as an adaptor protein for other signaling pathways. Although we were unable to demonstrate phosphorylation of endogenous Rai proteins following EGF or NGF treatment of neural cells, Rai can become phosphorylated upon overexpression and activation of other receptors (this report and reference 23).
The PI3K/Akt pathway is an important regulator of neuronal survival in both central and peripheral populations. In the case of sympathetic neurons of the SCG, survival induced by NGF is critically dependent upon an intact PI3K pathway (6). The PI3K/Akt pathway is also essential for the transmission of Ret-dependent survival signals in neuronal cell cultures (8, 35). Here we show that Rai overexpression per se promotes PI3K/Akt activation and neuronal cell survival. These Rai activities are further potentiated by Ret triggering. Accordingly, we have also found that treatment of cells with a PI3K inhibitor blocks Rai prosurvival effects (data not shown).
The SH2 domain of Rai mediates a growth factor-independent physical interaction with p85. In turn, upon Ret triggering, the SH2 domain of p85 establishes a further contact with tyrosine-phosphorylated Rai proteins. Our data suggest that the p85-Rai interaction is not direct but is mediated by other adaptors.
It has been shown that Shc recruits PI3K to activated Ret receptors through the Grb2-Gab2 complex. In rat sympathetic neurons, PI3K can be recruited to Ret Tyr-1062 by Shc, through the Grb2-Gab2 complex. Gab2 is an adaptor protein that binds p85 (1, 14). Several results, however, argue against the possibility that the Rai-p85 interaction is also mediated by Shc proteins: (i) expression of Shc proteins is low or undetectable in postmitotic neurons (where Rai is highly expressed) (3) and (ii) a stable Ret-Rai complex is also formed in the absence of Shc protein expression (our unpublished results with fibroblasts derived from Shc-null embryos). Alternatively, Rai might recruit p85 through Gab2. Notably, we have preliminarily demonstrated that Rai forms a complex with Gab2 following Ret activation (unpublished results).
Experimental evidence points to phosphorylation of the Tyr-1062 residue of Ret as the key event for propagating PI3K/Akt activating signals (8, 35). This autophosphorylation site serves as a docking site for the recruitment of multiple proteins to activated Ret. Binding of both FRS2 and Shc to Tyr-1062 has been implicated in the assembly of two distinct complexes on Ret, respectively, one leading to Ras activation through recruitment of Grb2/SOS and another leading to the PI3K/Akt pathway through recruitment of Grb2/Gab-1 followed by p85 (1, 14, 20). In addition, the binding of IRS1 (19) and p62dok family members (13) to Ret Tyr-1062 leads primarily to PI3K or Ras activation, respectively. Here we show that Rai, mainly through its PTB domain, is another ligand of Tyr-1062 of Ret. In this frame, it is intriguing that a natural Ret mutant (N1059) causing Hirschsprung's disease exerts a defective association with Rai. This suggests that a defect in the Ret-Rai pathway may contribute to the impaired development of enteric sympathetic neurons. Likewise, constitutive triggering of the Rai-PI3K pathway by oncogenic Ret mutants (Ret-MEN2A) may contribute to the neoplastic expansion of neural crest cells which are targeted by the MEN2 disease.
Our data propose a model whereby Rai proteins function as adaptors to facilitate recruitment of p85 by phosphorylated Tyr-1062 on activated Ret. According to this model, in response to GDNF treatment, a preexisting Rai/p85 complex is recruited to the phosphorylated Ret Tyr-1062 mainly through the Rai PTB domain. Upon receptor triggering, the stability of the p85-Rai interaction, which is mediated by the Rai SH2 domain in unstimulated cells, might be further increased via a direct contact of phosphorylated Rai with the p85 SH2 domains. This additional contact may also serve as a mechanism to modify the structure of the p85-p110 complex to potentiate p110 activation. Likely, another critical step in Ret-mediated potentiation of PI3K is the translocation of Rai-PI3K complexes from the cytosol to the plasma membrane where PI3K substrate lipids are located. The mechanisms through which the constitutive Rai-PI3K complex activates the PI3K/Akt pathway in the absence of receptor activation remain unexplained. Preliminary experiments from our laboratory indicate that treatment of neuronal cells with various toxic stimuli induces a redistribution of Rai proteins from the cytosol to other cellular compartments. Thus, it is possible that receptor-independent activation of p85 in Rai-expressing neuronal cells occurs through relocalization of the preexisting Rai-p85 complex.
Finally, we showed that Shc overexpression prolongs and potentiates the early peak (5 to 15 min) of Ras activation while Rai overexpression increases MAPK activation only at later time points (30 min). The two events (Shc- or Rai-induced MAPK activation) might be mechanistically unrelated, as only Shc proteins could be detected in stable complexes with Grb2. Since a number of published experiments suggest that formation of Shc-Grb2 complexes contribute to Ras activation, other mechanisms might be responsible for the ability of overexpressed Rai to increase late MAPK activation following GDNF treatment. Notably, recent findings suggest that PI3K activates the Ras-MAPK pathway, by acting both upstream of Ras and between Ras and ERK2 (40). In this report, inhibitors of PI3K are shown to suppress activation of both endogenous ERK2 and Ras in COS cells treated with physiological (1 to 2 ng/ml) doses of EGF. Therefore, the effect of Rai overexpression on the late peak of MAPK activation might be a consequence of its ability to increase PI3K activation following GDNF.
Thus, two different members of the Shc protein family, Shc and Rai, are recruited to the activated Ret receptor. Their association with Ret, mediated by the same site (e.g., Tyr-1062), is likely mutually exclusive. Therefore, Shc and Rai may regulate Ret signaling during different stages of neuronal development and elicit different functional consequences.
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ACKNOWLEDGMENTS |
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1059 Ret mutant, and V. Raker, A. Ventura, and K. Helin for helpful comments and critical readings of the manuscript. This work was supported by grants from AIRC and EC.
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FOOTNOTES |
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Present address: Istituto di Patologia Generale, Perugia University, 06100 Perugia, Italy.
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