This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, H.
Right arrow Articles by Kahn, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, H.
Right arrow Articles by Kahn, M. L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2003, p. 4764-4777, Vol. 23, No. 14
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.14.4764-4777.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Reciprocal Signaling by Integrin and Nonintegrin Receptors during Collagen Activation of Platelets

Hong Chen and Mark L. Kahn*

Division of Cardiology and Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Received 11 December 2002/ Returned for modification 24 February 2003/ Accepted 21 April 2003


arrow
ABSTRACT
 
Activation of platelets by exposed collagen after vessel wall injury is a primary event in the pathogenesis of stroke and myocardial infarction. Two collagen receptors, integrin {alpha}2ß1 and glycoprotein VI (GPVI), are expressed at similar levels on human and mouse platelets, but their individual roles during collagen activation remain poorly defined. Recent genetic and pharmacologic experiments have revealed an essential role for GPVI but have failed to define the role of {alpha}2ß1 or explain how two structurally distinct collagen receptors might function together to mediate platelet collagen responses. Discriminating the roles of these two collagen receptors is complicated by evidence suggesting that GPVI and platelet integrins may activate a common intracellular signaling pathway. To determine how {alpha}2ß1 and GPVI activate platelets in response to collagen, we have (i) examined collagen signaling conferred by expression of these receptors in hematopoietic cell lines; (ii) determined the effect of blocking each receptor on the activation of human platelets by collagen; (iii) generated low-GPVI mice in which the {alpha}2ß1/GPVI receptor ratio has been altered from 1:1 to 50:1 to expose {alpha}2ß1 function; (iv) studied the collagen responses of mouse platelets lacking LAT, an adaptor protein critical for GPVI but not integrin signaling; and (v) addressed the mechanism by which soluble collagens activate wild-type platelets. These studies demonstrate that {alpha}2ß1 requires inside-out signals to participate in collagen signaling and that {alpha}2ß1 is required for collagen activation of platelets when GPVI signals are reduced by blocking anti-GPVI antibody, low receptor number, specific disruption of the GPVI signaling pathway, or forms of collagen that bind weakly to GPVI relative to {alpha}2ß1. We propose a reciprocal two-receptor model of collagen signaling in platelets in which the nonintegrin receptor GPVI provides the primary collagen signal that activates and recruits the integrin receptor {alpha}2ß1 to further amplify collagen signals and fully activate platelets through a common intracellular signaling pathway. This model explains many of the genetic and pharmacologic observations regarding collagen signaling in platelets and demonstrates a novel mechanism by which hematopoietic cells integrate signaling by structurally distinct receptors that share a common ligand.


arrow
INTRODUCTION
 
Platelet activation in response to vessel wall injury is an initiating event in atherothrombotic diseases such as stroke and myocardial infarction (22). Collagen is a vessel wall protein known to directly activate platelets (38), and platelet activation by exposed collagen is believed to be an early and important step in the pathogenesis of these diseases. The molecular basis of platelet activation by collagen has been studied for more than 15 years with the identification of two major collagen receptors on mouse and human platelets: the integrin {alpha}2ß1 (34) and glycoprotein VI (GPVI), a receptor homologous to immune receptors that signals through the transmembrane signaling adaptor Fc gamma receptor (FcR{gamma}) (8). Identification of the roles of GPVI and {alpha}2ß1 during collagen activation of platelets is essential for understanding the pathogenesis of stroke and myocardial infarction and for the development of new therapies to treat these diseases.

Previous pharmacologic and genetic studies to define the roles of {alpha}2ß1 and GPVI during collagen activation of platelets have not yielded a clear picture of how these receptors work together to activate platelets in response to collagen. Early models proposed that collagen interaction with the high-affinity receptor {alpha}2ß1 was required for subsequent interaction with GPVI (2), but we have shown that heterologous expression of GPVI alone at a receptor density equivalent to that in platelets is sufficient to confer collagen adhesion and signaling (6). Loss of GPVI expression in mouse and human platelets results in a complete loss of collagen activation of platelets (26, 27, 29), identifying a necessary role for GPVI but leaving that of {alpha}2ß1 undefined. Early reports of human {alpha}2ß1 deficiency states demonstrated bleeding disorders and platelets with severely reduced collagen responses (19, 30). In contrast, mouse platelets lacking {alpha}2ß1 revealed almost no loss of aggregation responses to collagen (7, 11, 28). These studies are difficult to reconcile and may indicate important species differences, redundant receptor function, or a lack of participation by {alpha}2ß1 in collagen signaling. The difficulty in distinguishing contributions by {alpha}2ß1 and GPVI to collagen activation of platelets is compounded by their similar levels of expression on the platelet surface (6); by the possibility that the integrin {alpha}2ß1, like the fibrinogen receptor {alpha}IIbß3, requires inside-out activation for participation in collagen signaling (17); and by recent studies demonstrating that both receptors couple to the intracellular signaling proteins SYK, SLP-76, and PLC{gamma}2 (10, 12, 32).

To define the roles of GPVI and {alpha}2ß1 during collagen activation of platelets, we have combined several genetic and pharmacologic approaches. Heterologous expression of collagen receptors in hematopoietic cell lines expressing SYK, SLP-76, and PLC{gamma}2 conferred collagen signaling that was entirely GPVI dependent and unaffected by coexpression of {alpha}2ß1 unless the integrin was exogenously activated. Activated {alpha}2ß1, however, contributed to collagen signals, suggesting that the inability of {alpha}2ß1 alone to confer collagen signaling in cell lines may be due to a lack of integrin activation in these cells. To address the role of the two collagen receptors in human platelets, we used a novel blocking anti-GPVI antibody, 11A12, and the {alpha}2-blocking antibody 6F1 (9). Although 11A12 completely blocked collagen signaling conferred by GPVI in cell lines, neither antibody alone significantly blocked collagen activation of human platelets. Collagen activation could be completely blocked, however, by simultaneous exposure to both 11A12 and 6F1, a result that mirrors similar studies performed on mouse platelets (28) and reveals a role for both receptors that is conserved across species. To further expose the role of {alpha}2ß1 during collagen activation of platelets, we generated mice in which the {alpha}2ß1/GPVI ratio on platelets was altered from 1:1 to as high as 50:1 by reducing GPVI levels. Surprisingly, low-GPVI platelets lost responses to the high-affinity GPVI-specific agonists convulxin (CVX) and collagen-related peptide (CRP) but had relatively preserved collagen responses. Unlike those of wild-type platelets, however, the collagen responses of low-GPVI platelets were {alpha}2ß1 dependent, demonstrating a direct role for {alpha}2ß1 in platelet activation by collagen that is obscured by higher levels of GPVI. To determine whether {alpha}2ß1 mediated collagen signaling directly through outside-in integrin signals or indirectly through augmentation of GPVI signals, we studied the collagen response of mouse platelets lacking the transmembrane adaptor LAT. LAT is a lipid raft-associated transmembrane adaptor critical for GPVI but not integrin signaling (15). Despite normal levels of surface GPVI, collagen signaling in LAT-deficient platelets was {alpha}2ß1 dependent. Thus, reducing GPVI signaling from outside or inside the cell reveals a critical role for {alpha}2ß1 during collagen activation of platelets.

Finally, we have addressed the mechanism by which soluble collagens activate platelets. As previously reported, commercially available soluble collagens activated wild-type platelets, albeit at much higher concentrations than required for fibrillar collagen (11, 28, 35). Like GPVI-blocked, low-GPVI, and LAT-deficient platelet responses to fibrillar collagen, wild-type platelet responses to soluble collagen were {alpha}2ß1 dependent (11, 28, 35). Soluble collagens could not activate GPVI-deficient platelets, however, and soluble collagens supported adhesion of GPVI-expressing RBL-2H3 cells. Soluble collagens therefore provide another means of reducing but not ablating GPVI signaling to reveal a critical role for {alpha}2ß1.

Our studies support a model of collagen signaling in platelets in which two structurally distinct receptors regulate and amplify the response to exposed collagen in a reciprocal fashion. In this model GPVI is required to generate the first collagen signal that activates {alpha}2ß1 which then amplifies collagen signals by using many of the same intracellular signaling effectors. This model resolves many outstanding questions regarding the activation of platelets by different forms of collagen as well as the collagen activation of platelets from genetically modified mice. Reciprocal signaling by integrin and nonintegrin collagen receptors reveals a novel mechanism of functionally integrating receptor signaling in hematopoietic cells and suggests that therapeutic approaches to human vascular diseases based on inhibiting platelet collagen responses must address the role of both collagen receptors.


arrow
MATERIALS AND METHODS
 
Reagents and animals. CVX and CRP were from the same sources as described previously (6). Fibrillar type I collagen derived from equine tendon was obtained from Chronolog. Soluble collagens included rat tail type I collagen (Sigma), human placental type III collagen (Sigma), and human placental type I collagen (BD Biosciences). Anti-human GPVI mouse monoclonal antibodies were produced as previously described (6). Fluorescein isothiocyanate (FITC)-conjugated AK-7, anti-human integrin {alpha}2 antibody, and anti-rat integrin {alpha}2 monoclonal antibody, Ha1/29, were purchased from Pharmingen. TS2/16, anti-human integrin ß1 monoclonal antibody was from the American Type Culture Collection. Anti-human integrin {alpha}2 monoclonal antibody, 6F1, was a kind gift of Barry S. Coller. EMS16, a snake venom purified from Echis multisquamatus, was a kind gift of Cezary Marcinkiewicz. LAT-deficient mice were a kind gift of Larry Samelson. FcR{gamma}-deficient mice were obtained as previously described (6), and BALB/c mice were used as wild-type mouse controls.

Generation of HEL cells and RBL-2H3 cells expressing GPVI and/or integrin {alpha}2, or GPVI-RIIa. Stable clones of RBL-2H3 and HEL cells were obtained by using previously described methods (6).

Calcium signaling assays. RBL-2H3 and HEL cells were labeled with Fura-2am (Molecular Probes), and calcium signaling was detected as previously described (6). Platelet calcium assays were performed in an identical manner, except that 10 µg of Fura-2am/ml was added to human platelet-rich plasma obtained as previously described (6).

Aggregation and adhesion assays. Human and mouse platelet aggregation assays were performed as previously described (6). RBL-2H3 cell adhesion assays were performed as previously described (6).

Construction of the GPVI-RIIa chimeric gene. Extracellular domain of human GPVI was amplified from a plasmid containing GPVI cDNA with the following primer pair: 5'-ATCGGATCCATTTGAGGAACCATGTCTCCATC-3' and 5'-ATCCAAGCTTGGCGCGCCCTTGGTGTAGTACTGGCGG-3'. The amplified 0.8-kb fragment was subcloned into pBluescript KS(-) generating pBS-GPVI-0.8. The transmembrane domain and intracellular domain of Fc{gamma}RIIa was amplified from a plasmid containing Fc{gamma}RIIa cDNA with primer pairs (5'-ATCCACGCGTCCTCTTCACCAATGGGGATCATTGT-3' and 5'-ATCCCTCGAGTCAATGGTGATGGTGATGATGAC-3'). The amplified 0.4-kb fragments was subcloned into pBS-GPVI-0.8 at AscI and XhoI sites, generating plasmid pBS-GPVI-RIIA. The final chimeric construct GPVI-RIIa consists of the extracellular domain of GPVI and the transmembrane and intracellular domain of Fc{gamma}RIIa, and the correct construction was confirmed by sequencing.

Generation of GPVI-RIIa transgenic mice. The BamHI/XhoI 1.2-kb fragments of GPVI-RIIa was cloned into a retroviral vector (MIGR1) at the XhoI and BglII sites. To drive platelet-specific expression of GPVI-RIIa, the 2.4-kb EcoNI/SalI fragments of MIGR1-GPVI-RIIa containing GPVI-RIIa and green fluorescent protein (GFP) cDNA flanking an internal ribosomal entry site sequence was blunt ended with Klenow, ligated with NotI linker, and subcloned into pNASS-Ib{alpha}.GFP at NotI sites. The resulting plasmid pIb{alpha}-GPVI-RIIa-GFP was digested with AscI and SphI to release a fragment containing GPIb{alpha} promoter sequences driving the expression of GPVI-RIIa and GFP. Transgenic animals were generated by using standard oocyte injection protocols by the Transgenic Core Facility in the University of Pennsylvania. The expression of GFP in founder mouse tail blood was detected by flow cytometry. Transgene-positive founder lines were bred onto an FcR{gamma}-deficient mouse background.

Western blotting. Mouse and human platelets were pelleted as described for the calcium signaling assay. Mouse and human platelet pellets were lysed in 1x sample buffer, and Western blotting was performed with the anti-GPVI monoclonal antibody 6B12 as previously described (6).


arrow
RESULTS
 
Expression of GPVI but not {alpha}2ß1 confers collagen signaling in RBL-2H3 cells. The RBL-2H3 cell line is a rat hematopoietic cell line that expresses the intracellular signaling proteins SYK, SLP-76, and PLC{gamma} but is null for GPVI and expresses very low levels of the integrin {alpha}2 (Fig. 1A) (6, 20). We have previously shown that expression of GPVI in the RBL-2H3 cell line is sufficient to confer collagen signaling when GPVI receptor density matches that in human platelets (6). To determine whether expression of high levels of {alpha}2ß1 alone was sufficient to confer collagen responses in these cells, we generated RBL-2H3 cells in which human {alpha}2 was expressed in association with rodent ß1 (RBL.{alpha}2, Fig. 1A), a combination previously demonstrated to form a functional collagen receptor (42). To test whether expression of {alpha}2ß1 augmented collagen responses in GPVI-expressing cells, we also generated RBL-2H3 cell lines that coexpressed human GPVI and {alpha}2 (RBL.GPVI.{alpha}2). Expression of {alpha}2ß1 on RBL-2H3 cells conferred collagen adhesion under static conditions that could be blocked by the {alpha}2-blocking snake venom EMS-16 (23), by the anti-{alpha}2 antibody 6F1, or by the removal of cation with EDTA (Fig. 1B). As predicted by previous experiments performed with GPVI-expressing RBL-2H3 cells (6), adhesion of RBL.GPVI.{alpha}2 cells to collagen was also directly mediated by GPVI and could only be partially blocked with anti-{alpha}2 blocking agents or with EDTA (Fig. 1B). Despite adhesion to collagen, no calcium signaling was detected in RBL.{alpha}2 cells in response to collagen, although RBL.GPVI.{alpha}2 cells exhibited robust calcium responses to collagen (Fig. 1C). To detect a contribution by {alpha}2ß1 to collagen signaling in cells expressing both receptors, RBL.GPVI.{alpha}2 cells were exposed to EMS16 or 6F1 (Fig. 1D). Surprisingly, no effect on collagen signaling was noted with blockade of {alpha}2ß1.



View larger version (25K):
[in this window]
[in a new window]
  		FIG. 1. Expression of GPVI, but not integrin {alpha}2ß1, confers collagen signaling in RBL-2H3 cells. (A) Expression of {alpha}2 integrin in wild-type (RBL) and {alpha}2-expressing (RBL.{alpha}2) RBL-2H3 cells. Endogenous rat {alpha}2 was measured by using the anti-rat {alpha}2 antibody Ha1/29 with secondary FITC-conjugated anti-hamster antibody in wild-type RBL-2H3 cells (left) and expressed human {alpha}2 measured by using FITC-conjugated anti-human antibody AK-7 in RBL.{alpha}2 cells (right). (B) Expression of {alpha}2 or GPVI confers static adhesion to collagen. RBL-2H3 cells expressing either integrin {alpha}2 (RBL.{alpha}2) or integrin {alpha}2 plus GPVI (RBL.GPVI.{alpha}2) were analyzed for adhesion to a collagen-coated plate for 30 min with or without 6F1 (10 µg/ml), EMS-16 (10 nM), or EDTA (5 mM). Note that the binding of RBL.{alpha}2 cells to collagen was cation dependent and inhibited by the anti-{alpha}2 agents 6F1 and EMS16. In contrast, the binding to collagen of RBL.GPVI.{alpha}2 cells was resistant to these treatments. (C) GPVI but not {alpha}2ß1 confers collagen signaling in RBL-2H3 cells. RBL.{alpha}2 and RBL.GPVI.{alpha}2 cells were labeled with Fura-2 and intracellular calcium responses to collagen (10 µg/ml) or thrombin (10 nM) was measured. (D) Blockade of {alpha}2ß1 does not reduce collagen signaling in RBL-2H3 cells expressing both GPVI and {alpha}2ß1. RBL.GPVI.{alpha}2-mediated calcium signaling in response to collagen (10 µg/ml) was measured in untreated cells and in cells treated with high concentrations of EMS16 (500 nM) or 6F1 (100 µg/ml). (E) The anti-GPVI antibody 11A12 blocks GPVI-mediated collagen adhesion in RBL-2H3 cells. RBL-2H3 cells expressing high levels of GPVI (RBL.GPVI-173 [6]) were assayed for adhesion to collagen-coated plates for 15 min with or without pretreatment with 3, 10, or 30 µg of 11A12/ml. (F) Anti-GPVI antibody 11A12 blocks GPVI-mediated collagen signaling in RBL-2H3 cells. Calcium signals in response to collagen (10 µg/ml) were measured in RBL.GPVI-173 cells after treatment with 11A12 (20 µg/ml) or nonspecific mouse immunoglobulin G (IgG) (20 µg/ml). (G) Blockade of GPVI with 11A12 inhibits signaling in RBL-2H3 cells expressing both GPVI and {alpha}2ß1. Calcium signals in response to collagen (10 µg/ml) were measured in RBL.GPVI.{alpha}2 cells after treatment with 11A12 (20 µg/ml) or nonspecific mouse IgG (20 µg/ml).

To confirm that collagen signals in RBL-2H3 cells were dependent upon GPVI-collagen interaction, a blocking anti-human GPVI monoclonal antibody, 11A12, was generated. The antibody 11A12 blocks both collagen adhesion and collagen signaling by GPVI-expressing RBL-2H3 cells at low antibody concentrations (Fig. 1E and F). Like GPVI-expressing RBL-2H3 cells, the collagen responses of RBL-2H3 cells expressing both GPVI and {alpha}2ß1 were blocked by 11A12 (Fig. 1G). These experiments confirm that collagen signaling in RBL-2H3 cells is conferred by GPVI but not {alpha}2ß1 even if both receptors are present.

Exogenous activation of {alpha}2ß1 confers collagen signals that are resistant to the anti-GPVI antibody 11A12 in HEL cells. Platelet integrins are maintained in an inactive state and do not bind soluble ligand until they are activated by intracellular signals through a process known as inside-out signaling (31). Studies of the fibrinogen receptor {alpha}IIbß3 in the megakaryocytic HEL cell line have revealed that these cells, unlike platelets, are unable to generate the inside-out signals required to activate this integrin (13). These studies suggested that the failure of RBL.{alpha}2 and RBL.GPVI.{alpha}2 to exhibit {alpha}2ß1-dependent collagen signaling responses might be due to an inability to activate {alpha}2ß1. To test the possibility that {alpha}2ß1 requires activation to participate in collagen signaling, we used the human megakaryocytic HEL cell line and the TS2/16 antibody, an anti-human ß1 antibody that, when bound to {alpha}2ß1, increases the integrin's affinity for ligand (1). Unmanipulated HEL cells express low levels of {alpha}2ß1 and GPVI that may be augmented by treatment with phorbol myristate acetate (PMA) (3, 4) (Fig. 2A). Neither unmanipulated nor PMA-treated HEL cells demonstrated collagen responses (data not shown). Since collagen responses in RBL-2H3 cells are highly dependent upon GPVI receptor density (6), we stably transfected HEL cells with a GPVI-expressing vector to further boost the level of GPVI (HEL.GPVI, Fig. 2A). When treated with PMA, HEL.GPVI cells generated small calcium signals in response to collagen but these could be significantly augmented by activating {alpha}2ß1 with TS2/16 (Fig. 2B). Unlike collagen-responsive GPVI-expressing RBL-2H3 cells, however, collagen signals in TS2/16-treated HEL.GPVI/PMA cells were resistant to the blocking anti-GPVI antibody 11A12 and could only be blocked with the blocking anti-GPVI antibody 11A12 and anti-{alpha}2 antibody 6F1. These results suggest that {alpha}2ß1 may participate in collagen signaling responses in an activation-dependent manner. By extension, the failure to confer signaling in RBL-2H3 cells with expression of {alpha}2ß1 is likely to be due to an inability to activate the integrin.



View larger version (30K):
[in this window]
[in a new window]
  		FIG. 2. The integrin {alpha}2ß1 participates in collagen responses in HEL cells in an activation-dependent manner. (A) Generation of HEL cells expressing high levels of GPVI and {alpha}2ß1. To generate HEL cells expressing high levels of the collagen receptors GPVI and {alpha}2ß1, unmanipulated or GPVI-transfected cells were matured with PMA treatment. GPVI and {alpha}2 surface expression was detected by using FITC-11A12 and FITC-AK-7, respectively. HEL, unmanipulated HEL cells; HEL/PMA, PMA-treated HEL cells; HEL.GPVI, HEL cells in which human GPVI is stably expressed through a transgene; HEL.GPVI/PMA, HEL.GPVI treated with PMA. (B) {alpha}2ß1 contributes to collagen signaling in HEL cells after exogenous activation. Calcium signaling in response to collagen (10 µg/ml) was measured in HEL.GPVI/PMA cells untreated or pretreated with the activating anti-ß1 antibody TS2/16 (50 µg/ml, top panels). Calcium signals in TS2/16-treated HEL.GPVI/PMA cells in response to collagen (10 µg/ml) were also measured in the presence of the blocking anti-GPVI antibody 11A12 (30 µg/ml) with or without the blocking anti-{alpha}2 antibody 6F1 (30 µg/ml, lower panels). Note that the inhibition of collagen signaling conferred by TS2/16 treatment requires blockade of {alpha}2ß1 in addition to GPVI.

Blockade of both {alpha}2ß1 and GPVI is required to inhibit collagen activation of human platelets. Augmentation of collagen signaling by exogenously activated {alpha}2ß1 in HEL cells suggested that in platelets both receptors are likely to participate in collagen signaling. To test the role of each receptor for collagen activation of human platelets, aggregation studies were performed with blocking antibodies against {alpha}2, GPVI, or both. At higher concentrations of collagen (>10 µg/ml) very little inhibition was seen with blockade of either {alpha}2ß1 or GPVI alone, but virtually complete, synergistic inhibition was observed with simultaneous blockade of the two receptors (Fig. 3A). Similar results were obtained through analysis of calcium signaling induced by collagen, although small reductions in calcium flux are detectable with inhibition of each receptor alone (Fig. 3B). These results are consistent with those obtained in the HEL cell system with activated {alpha}2ß1 and demonstrate the participation of both receptors during collagen activation of platelets.



View larger version (20K):
[in this window]
[in a new window]
  		FIG. 3. Inhibition of collagen activation of human platelets requires blockade of both GPVI and {alpha}2ß1. (A) Inhibition of collagen-induced platelet aggregation requires treatment with both the anti-GPVI antibody 11A12 and the anti-{alpha}2 antibody 6F1. Gel-filtered human platelets (2 x 108/ml) were treated with 30 µg of 11A12/ml, 30 µg of 6F1/ml, or both for 5 min prior to measuring platelet aggregation in response to 30 µg of collagen/ml. Similar results were obtained by using 10 and 100 µg of collagen/ml (data not shown). (B) Inhibition of collagen-induced calcium signaling requires both the anti-GPVI antibody 11A12 and the anti-{alpha}2 antibody 6F1. Calcium signaling in response to collagen (10 µg/ml) was measured by using the same antibody conditions as in panel A. Note the synergistic inhibitory effect of blocking both GPVI and {alpha}2ß1 on platelet activation by collagen.

Low-GPVI platelets signal in response to collagen but not the GPVI-specific agonists CVX or CRP. Although our experiments supported a significant role for {alpha}2ß1 during collagen activation of human platelets, both mouse and human GPVI-deficient platelets are unresponsive to collagen (26, 27), and {alpha}2ß1-deficient platelets have near-normal collagen responses (11, 28). Given the requirement for {alpha}2ß1 activation observed in HEL cells, we hypothesized that during collagen stimulation {alpha}2ß1 might require GPVI for inside-out activation and, once activated, perform a signaling function redundant with that of GPVI. To expose such a role for {alpha}2ß1 during collagen signaling in platelets, we generated mice with altered collagen receptor stoichiometries such that the GPVI/{alpha}2ß1 ratio was radically skewed in favor of {alpha}2ß1. Since GPVI expression requires coexpression of the FcR{gamma} chain, FcR{gamma}-deficient mouse platelets lack surface GPVI and are completely unresponsive to collagen (27). It is not currently known whether mouse platelets express FcR{gamma} partners other than GPVI. To generate low-GPVI platelets without rescuing other FcR{gamma} partners, we therefore engineered transgenic mice expressing a GPVI-Fc{gamma}RIIa chimera (GPVI-RIIa) under control of the GPIb{alpha} promoter and crossed these transgenics onto an FcR{gamma}-deficient background (Fig. 4A). The GPVI-RIIa chimeric receptor contains the extracellular domain of human GPVI and the transmembrane and intracellular domains of the human platelet Fc receptor Fc{gamma}RIIa. Fc{gamma}RIIa is known to signal through the same intracellular signaling pathway as GPVI-FcR{gamma} and is expressed independently of FcR{gamma} in human but not mouse platelets (5, 24). Using this strategy we generated two lines of mice, M5 and C14, whose platelets express levels of GPVI-RIIa that are ca. 2% that of GPVI on normal human platelets and that we designate "low-GPVI" platelets (Fig. 4B). Although only results obtained by using M5-derived low-GPVI platelets are shown, M5 and C14 platelets exhibited identical responses to CVX, CRP, and collagen (data not shown). Low-GPVI platelets express wild-type levels of {alpha}2ß1 (data not shown).



View larger version (21K):
[in this window]
[in a new window]
  		FIG. 4. Integrin {alpha}2ß1 is required for collagen responses in low-GPVI mouse platelets. (A) Schematic of the GPVI-RIIa transgene used to generate mice with low-GPVI platelets. GPIb{alpha}, the GPIb{alpha} promoter; EC, human GPVI extracellular domain; TM/IC, Fc{gamma}RIIa transmembrane and intracellular domains; IRES, internal ribosomal entry site. (B) Low-GPVI platelets express GPVI-RIIa at a level equal to ca. 2% of the GPVI on human platelets. To generate low-GPVI platelets, two GPVI-RIIa transgenic founder lines, M5 and C14, were bred onto an FcR{gamma}-deficient background to eliminate native GPVI. The expression level of GPVI-RIIa in these two lines was measured by Western blotting with an antibody that recognizes the extracellular domain of human GPVI (clone 6B12) and compared to that of human platelet lysates from various numbers of platelets. Note the increased size of GPVI-RIIa relative to wild-type human GPVI due to the presence of the longer Fc{gamma}RIIa intracellular tail. The 50-kDa band detected in both wild-type human and transgenic mouse platelets is also detected in FcR{gamma}-deficient platelets that lack GPVI and is a background band. (C) Low-GPVI platelets respond to collagen but not to the GPVI-specific agonists CVX or CRP. The aggregation responses of low-GPVI (GPVI-RIIa+/+; FcR{gamma}-/-) and wild-type platelets (3 x 108/ml) to CVX (10 nM), CRP (150 µg/ml), and collagen (30 µg/ml) are shown. Each experiment is representative of three to five experiments with similar results. (D) The GPVI-RIIa receptor is efficiently activated by CVX but not collagen in RBL-2H3 cells. GPVI-RIIa-expressing RBL-2H3 cells were tested for calcium signaling in response to CVX (10 nM) or collagen (10 µg/ml). Note that GPVI-RIIa responds preferentially to the high-affinity GPVI ligand CVX. (E) Low-GPVI but not wild-type platelet collagen responses are dependent upon {alpha}2ß1. Low-GPVI and wild-type platelets were pretreated with the blocking anti-{alpha}2 antibody Ha1/29 (10 µg/ml) or control IgG (10 µg/ml) prior to stimulation with collagen (30 µg/ml). This experiment is representative of three experiments with similar results.

Unlike wild-type platelets, low-GPVI platelets exhibited no aggregation response to the high-affinity GPVI ligands CVX or CRP (Fig. 4C). Remarkably, despite a virtually complete loss of GPVI-specific responses, low-GPVI platelets had relatively preserved collagen responses (Fig. 4C). Low-GPVI platelets did exhibit a right shift in their collagen dose response that was in proportion to the level of GPVI-RIIa receptors expressed (Fig. 4C and data not shown). To rule out the possibility that collagen might be a more potent agonist for the GPVI-RIIa chimeric receptor than for wild-type GPVI, we characterized the responses of GPVI-RIIa to collagen and CVX in GPVI-RIIa-expressing RBL-2H3 cells. As previously reported for GPVI (6), expression of GPVI-RIIa at modest levels in RBL-2H3 cells conferred signaling responses to the high-affinity GPVI ligand CVX but not to collagen (Fig. 4D). Thus, preferential preservation of collagen responses in low-GPVI platelets is not due to a difference in ligand preference between GPVI and GPVI-RIIa receptors.

Collagen activation of low-GPVI platelets is mediated by {alpha}2ß1. Signaling in response to collagen but not GPVI-specific agonists in low-GPVI platelets is consistent with the function of a second receptor that recognizes collagen but not CVX or CRP. To determine whether {alpha}2ß1 is responsible for collagen signaling in low-GPVI platelets, we compared the sensitivity of wild-type and low-GPVI platelets to {alpha}2ß1 blockade with the blocking anti-{alpha}2 monoclonal antibody Ha1/29 (25). As observed in human platelets (Fig. 3A), blockade of {alpha}2ß1 in wild-type mouse platelets results only in a slightly delayed aggregation response to collagen (Fig. 4E). In contrast, blockade of {alpha}2ß1 completely blocked low-GPVI platelet responses to collagen (Fig. 4E). Interestingly, analysis of a third transgenic line whose platelets express levels of GPVI-RIIa that are ca. 10% that of GPVI on normal human platelets revealed an intermediate phenotype in which inhibition of {alpha}2ß1 significantly reduced but did not ablate platelet collagen responses (data not shown). These results demonstrate that collagen responses in low-GPVI platelets are mediated by the integrin {alpha}2ß1 and suggest that as GPVI levels increase the role of {alpha}2ß1 becomes increasingly redundant.

Despite normal levels of GPVI, collagen activation of LAT-deficient platelets also requires {alpha}2ß1. The ability to activate low-GPVI platelets with collagen but not CVX or CRP mirrors the response of platelets lacking the transmembrane adaptor LAT (15). As previously reported, LAT-deficient platelets exhibit only a mild reduction in platelet collagen responses but a profound reduction in the response to the GPVI-specific agonists CVX and CRP (Fig. 5A). A comparison of the dose-response curves of wild-type and LAT-deficient platelet aggregation reveals a 3-fold rightward shift for collagen and 100- and 1,000-fold rightward shifts for CVX and CRP, respectively (Fig. 5B). To determine whether the collagen responses of LAT-deficient platelets are {alpha}2ß1 dependent, we tested the effect of the blocking anti-{alpha}2 antibody Ha1/29. Like low-GPVI platelets, even strong LAT-deficient platelet responses to collagen were virtually ablated by {alpha}2ß1 inhibition (Fig. 5C). The requirement for {alpha}2ß1 is not related to a reduction in GPVI receptor number since the surface expression of GPVI and {alpha}2ß1 receptors on LAT-deficient platelets was indistinguishable from that on wild-type platelets (data not shown). These results demonstrate that reducing GPVI signaling in platelets from outside the cell by lowering the receptor number or from inside the cell by impairing GPVI signaling reveals a critical role for {alpha}2ß1 during collagen activation of platelets.



View larger version (20K):
[in this window]
[in a new window]
  		FIG. 5. {alpha}2ß1 is required for collagen responses in LAT-deficient mouse platelets. (A) Aggregation dose-responses of wild-type and LAT-deficient platelets to collagen and the GPVI-specific agonists CVX and CRP. Gel-filtered platelets from wild-type (LAT+/+) and LAT-deficient (LAT-/-) platelets were exposed to various concentrations of collagen, CVX, and CRP, and platelet aggregation was measured. Note the small right shift observed in LAT-deficient platelets in response to collagen compared to the large right shifts observed for CVX and CRP. This experiment is representative of 3 experiments with similar results. (B) The 50% effective concentration (EC50) of platelet aggregation responses of wild-type (+/+) and LAT-deficient (-/-) platelets. The EC50s were calculated based on the aggregation responses to collagen, CVX, and CRP shown in panel A. Note that the increases in EC50 for LAT-deficient platelet responses to the GPVI-specific agonists CVX and CRP vastly exceed that for collagen. (C) Collagen responses of LAT-deficient platelets require {alpha}2ß1. Wild-type (LAT+/+) and LAT-deficient (LAT-/-) platelets were pretreated with 30 µg of the blocking anti-{alpha}2 antibody Ha1/29 or IgG control/ml, and the response to various concentrations of collagen was measured. Note that even strong responses of LAT-deficient platelets to collagen are ablated by inhibition of {alpha}2ß1, whereas wild-type platelets exhibit only a slight delay in the aggregation response.

Activation of wild-type platelets by soluble collagens is {alpha}2ß1 dependent but soluble collagens interact with GPVI. The partial digestion of fibrillar type I and type III collagen yields more soluble forms of collagen that have been reported to bind preferentially to {alpha}2ß1 and not GPVI (11, 16, 17, 28, 35), suggesting that these forms of collagen might activate wild-type platelets in a manner analogous to the activation of low-GPVI and LAT-deficient platelets by fibrillar collagen. As previously reported (11, 28, 35), two commercially available forms of soluble type I collagen (derived from rat tail and human placenta) and a soluble type III collagen (derived from human placenta) stimulate aggregation of wild-type mouse platelets but do so much less efficiently than fibrillar collagen (Fig. 6A). Thus, wild-type platelet responses to soluble collagens are right shifted in a manner similar to that of low-GPVI and LAT-deficient platelet responses to fibrillar collagen. Like low-GPVI and LAT-deficient platelet responses to fibrillar collagen, wild-type platelet responses to soluble collagens are sensitive to blockade by the anti-{alpha}2 antibody (Fig. 6B) (11, 28, 35). However, both fibrillar and soluble collagen aggregation responses are lost in FcR{gamma}-deficient or GPVI-blocked platelets (Fig. 6C) (11, 28), suggesting that some residual interaction with GPVI is required for soluble collagen to activate platelets. To test whether soluble collagens can interact with GPVI, we performed static adhesion assays with wild-type and GPVI-expressing RBL-2H3 cells. Expression of GPVI conferred adhesion to soluble collagens, although adhesion was weaker than that obtained with more fibrillar collagen (Fig. 6D). These results demonstrate that although activation of platelets by soluble type I and type III collagens requires {alpha}2ß1, soluble collagens can bind GPVI weakly, and GPVI is required for activation of platelets by soluble collagens.



View larger version (24K):
[in this window]
[in a new window]
  		FIG. 6. Wild-type platelet responses to soluble collagens are {alpha}2ß1-dependent but soluble collagens interact with GPVI. (A) Aggregation responses of wild-type platelets to fibrillar and soluble collagens. Aggregation dose-responses of wild-type mouse platelets to two forms of soluble type I collagen (derived from rat tail and human placenta) and one form of soluble type III collagen (derived from human placenta) are shown in comparison to fibrillar type I collagen. Note that wild-type platelet responses to soluble collagens are right shifted up to 100-fold. (B) Aggregation responses of wild-type platelets to soluble but not fibrillar collagen require {alpha}2ß1. The effects of the blocking anti-{alpha}2 antibody Ha1/29 (anti-{alpha}2) and nonspecific hamster IgG (Ham IgG) on the response of wild-type platelets to three forms of soluble collagen and to type I fibrillar collagen are shown. Note that wild-type platelet responses to soluble collagens but not fibrillar collagen require {alpha}2ß1. Similar results were obtained with human platelets and the 6F1 antibody (data not shown). (C) Activation of platelets by soluble and fibrillar collagens requires GPVI-FcR{gamma}. FcR{gamma}-deficient platelet responses to activating concentrations of fibrillar and soluble collagens are shown in comparison to the G protein-coupled receptor agonist ADP. Note that soluble collagen responses require GPVI as well as {alpha}2ß1. (D) GPVI mediates adhesion to type I and III soluble collagens. Wild-type RBL-2H3 cells and RBL-2H3 cells expressing GPVI (RBL.GPVI) were analyzed for adhesion to plates coated with fibrillar type I collagen or soluble type I and III collagens. Note that expression of GPVI confers adhesion to soluble collagen as well as to fibrillar collagen, but to a lesser extent.


arrow
DISCUSSION
 
Previous studies of human and mouse platelets have yielded a complex picture of the platelet response to collagen. Two important collagen receptors, integrin {alpha}2ß1 and GPVI, have been identified, but whether and how they function together during collagen activation of platelets has been unclear. In the present study we utilized a number of methods, including heterologous expression in cell lines, blocking monoclonal antibodies, genetic experiments in mice, and different forms of collagen to define the function of each receptor. Our results support a unique two-receptor system in which integrin and nonintegrin collagen receptors function in a reciprocal manner to activate platelets in response to collagen.

Our studies address two outstanding issues regarding collagen activation of platelets: the sufficient roles of GPVI and {alpha}2ß1 and whether platelet collagen receptors function independently or in an integrated manner. Analysis of collagen receptor-expressing RBL-2H3 cells demonstrates that GPVI but not {alpha}2ß1 is sufficient to confer collagen signaling, a result consistent with the preservation of collagen responses in {alpha}2ß1-deficient but not GPVI-deficient mouse platelets (7, 11, 27, 28). The inability of {alpha}2ß1 to confer collagen signaling and the complete loss of collagen signaling observed in GPVI-deficient platelets may indicate that {alpha}2ß1 functions only as an adhesive receptor for collagen. Alternatively, {alpha}2ß1 may require inside-out activation to participate in collagen signaling, a process that may be deficient in RBL-2H3 cells (13) and in which GPVI might be required to generate the activating signal in an experimental system in which collagen was the sole agonist present. To expose an inside-out signaling defect in cell lines, we exogenously activated {alpha}2ß1 by using the TS2/16 antibody in HEL cells that express both {alpha}2ß1 and GPVI. TS2/16-mediated {alpha}2ß1 activation generated collagen signals that, in contrast to those in untreated cells, could not be inhibited by a blocking anti-GPVI antibody. These studies support previous work demonstrating an increase in the binding of soluble collagen (a relatively specific {alpha}2ß1 ligand, discussed further below) after platelet activation (17) and suggest that {alpha}2ß1 participates directly in collagen signaling in an activation-dependent manner.

If GPVI alone is sufficient for collagen signaling and {alpha}2ß1 requires inside-out activation signals generated by GPVI to participate in collagen signaling, the role of {alpha}2ß1 might only be exposed by drastically reducing but not ablating GPVI signals. To reduce but not eliminate GPVI signaling in platelets, we used four approaches: (i) pharmacologic inhibition of GPVI with a blocking anti-GPVI antibody; (ii) generation of mice with platelets expressing very low levels of GPVI; (iii) selective interruption of GPVI but not integrin signaling with platelets lacking LAT, a lipid raft-associated transmembrane adaptor; and (iv) activation of wild-type platelets by soluble collagens that are relatively but not absolutely specific ligands for {alpha}2ß1. Remarkably, although these approaches reduce GPVI signaling in distinct ways from both outside and inside the cell, they result in virtually identical phenotypes: severe loss of platelet responses to GPVI-specific agonists but relatively preserved platelet collagen responses that are dependent upon {alpha}2ß1 function.

Inhibition of human platelet collagen responses required blockade of both GPVI and {alpha}2ß1, a result identical to that observed with blocking anti-mouse GPVI and anti-mouse {alpha}2 antibodies (28, 36). The inability of anti-GPVI antibodies to completely block platelet collagen responses in both species is most likely due to the fact that pharmacologic inhibition, unlike genetic ablation, is not absolute. GPVI-blocked platelets therefore retain enough GPVI signaling to activate {alpha}2ß1, which then supports collagen responses unless it is simultaneously blocked. These results reveal a significant role for {alpha}2ß1 during collagen activation of platelets and suggest that pharmacologic approaches designed to inhibit these responses may require blockade of both integrin and nonintegrin receptors. These results also provide evidence for the conservation of collagen receptor function across species and suggest that there is no need to postulate the existence of two distinct GPVI-binding sites for collagen to explain prior results with blocking anti-GPVI antibodies (36).

To genetically expose the role of {alpha}2ß1, we generated mice with platelets in which the {alpha}2ß1/GPVI receptor ratio is shifted radically in favor of {alpha}2ß1, while retaining a small number of GPVI receptors to provide inside-out signals for {alpha}2ß1 activation during collagen exposure (these are referred to here as low-GPVI platelets). Low-GPVI platelets expressing as few as 30 to 40 receptors per platelet (6) exhibited relatively preserved aggregation responses to collagen despite the loss of aggregation responses to the high-affinity GPVI-specific ligands CVX or CRP. Unlike wild-type, but similar to GPVI-blocked human platelets, low-GPVI platelet collagen responses were {alpha}2ß1 dependent, a result supported by a recent study of platelets with a less drastic reduction in GPVI receptor density (37). Thus, reducing GPVI-mediated collagen signals either through pharmacologic blockade or through genetic reduction in receptor number results in collagen signals that are supported by the integrin {alpha}2ß1.

How does {alpha}2ß1 support collagen responses in GPVI-blocked and low-GPVI platelets? {alpha}2ß1-dependent signals may be explained either through a direct and/or an indirect mechanism. In a direct mechanism, activated {alpha}2ß1 binds collagen and generates independent outside-in signals that are sufficient to fully activate platelets. In an indirect mechanism, activated {alpha}2ß1 functions as a coreceptor to enhance signaling through GPVI or an unidentified collagen receptor. To distinguish between direct {alpha}2ß1-mediated signaling and indirect {alpha}2ß1-mediated augmentation of GPVI signaling, we studied platelets lacking LAT, a lipid raft-associated transmembrane adaptor critical for GPVI but not integrin signal transduction.

Recent studies of integrin outside-in signaling in platelets have revealed that both the integrin receptor for fibrinogen ({alpha}IIbß3) and GPVI-FcR{gamma} signal through the non-receptor tyrosine kinase SYK (40), the adaptor SLP-76 (14), and the phospholipase PLC{gamma}2 (39). Recent studies of platelet spreading on the {alpha}2ß1-specific peptide ligand GFOGER suggest that {alpha}2ß1 also utilizes these signaling molecules during outside-in signaling (12). Thus, platelets lacking SYK, SLP-76, or PLC{gamma}2 are deficient in both fibrinogen spreading and activation by GPVI-specific agonists in addition to being unresponsive to collagen. In contrast, LAT-deficient platelets exhibit an almost complete loss of signaling responses to the GPVI-specific ligands CVX and CRP but spread normally on fibrinogen (Fig. 5) (15, 33). Thus, the transmembrane adaptor LAT is a signaling protein utilized preferentially by GPVI-FcR{gamma} but not by integrins in platelets. This is consistent with studies demonstrating that during collagen stimulation of platelets GPVI but not {alpha}2ß1 associates with lipid rafts (21, 39; data not shown), where LAT is exclusively localized (41). It is also consistent with the observation that intracellular signaling responses to collagen in GPVI-blocked platelets are identical to those in untreated platelets except for a nearly complete lack of LAT phosphorylation (36). Thus, our finding that LAT-deficient platelet collagen responses are mediated by {alpha}2ß1 despite normal levels of surface GPVI is most consistent with direct activation of platelets by {alpha}2ß1 outside-in signals.

Our studies support a model of collagen signaling in platelets in which an integrin and a nonintegrin receptor function in a reciprocal fashion to generate common activating intracellular signals in response to a common ligand (Fig. 7). In this model, the nonintegrin receptor GPVI is required to generate the first signal, S1, which engages the integrin {alpha}2ß1 through inside-out activation. In the setting of high GPVI receptor density, unlimited access to collagen, and unimpaired GPVI signaling, GPVI-mediated signals are sufficient to fully activate platelets. However, when the GPVI S1 is reduced by GPVI receptor blockade, low GPVI receptor density, or impaired GPVI signaling, the integrin {alpha}2ß1 is required to generate subsequent signals, S2, for full platelet activation. Significantly, this model does not exclude an {alpha}2ß1 coreceptor function by which it might also augment GPVI-mediated signals. However, coreceptor function alone cannot explain the requirement for {alpha}2ß1 in LAT-deficient platelets where GPVI is expressed at a high level. This model fits the accumulated cellular and genetic data regarding collagen activation of platelets: (i) platelets lacking GPVI or FcR{gamma} have lost the ability to activate {alpha}2ß1 in response to collagen and therefore cannot respond to collagen despite normal {alpha}2ß1 expression; (ii) {alpha}2ß1-deficient platelets express high enough levels of GPVI to make {alpha}2ß1 unnecessary for collagen signaling under experimental conditions using collagen suspensions; (iii) SYK, SLP-76, and PLC{gamma}2 are required for signaling downstream of both collagen receptors; and (iv) LAT-deficient platelets have lost most but not all GPVI signaling and thus are able to activate {alpha}2ß1 and generate {alpha}2ß1-dependent signals. Since the reports of {alpha}2ß1-deficient human individuals with reduced platelet collagen responses preceded the ability to measure GPVI levels (19, 30), it is tempting to speculate that these individuals may have also had reduced GPVI function.



View larger version (22K):
[in this window]
[in a new window]
  		FIG. 7. Reciprocal model of collagen signaling by GPVI and integrin {alpha}2ß1 in platelets. A proposed model of collagen signaling mediated by GPVI and integrin {alpha}2ß1 is shown. GPVI is postulated to provide the first collagen signal, S1, that mediates inside-out activation of {alpha}2ß1. In the presence of normal levels of GPVI, large amounts of collagen and LAT, the GPVI-derived S1 is sufficient for platelet activation by fibrillar collagen. Activated {alpha}2ß1 is postulated to generate a second collagen signal, S2, that is LAT independent and sufficient to activate platelets. {alpha}2ß1-generated signals are proposed to be required when GPVI signaling is low, e.g., due to antibody blockade, low receptor expression, loss of LAT, or exposure to soluble collagens that bind preferentially to {alpha}2ß1. Both GPVI and {alpha}2ß1 are believed to activate SYK, SLP-76, and PLC{gamma}2. The position of LAT is meant to demonstrate its critical role downstream of GPVI but not {alpha}2ß1 rather than to indicate that it lies upstream of SYK or SLP-76.

Our model predicts that a pure {alpha}2ß1 ligand would be unable to independently bind or activate resting platelets due to an inability to generate the signal required to activate the integrin (i.e., S1). Soluble collagens, generated by partial enzymatic digestion of fibrillar type I or type III collagen to a form lacking the complete quaternary structure of native banded collagen, have been described as such a ligand (11, 16, 17, 28, 35). Significantly, type III collagen that has been highly purified to remove all traces of fibrillar collagen has been demonstrated to behave as an {alpha}2ß1-selective ligand and bind platelets only after exogenous activation of the {alpha}2ß1 integrin with TS2/16 antibody or endogenous activation by other platelet receptors (16, 17). In contrast, other forms of soluble collagen have been reported to mediate platelet adhesion and activation independently but require {alpha}2ß1 to do so (Fig. 6) (11, 28, 35). A straightforward explanation for these observations is that forms of soluble collagen capable of activating platelets retain a small amount of GPVI interaction that is sufficient to activate {alpha}2ß1. This mechanism is supported by the observation that soluble collagens require GPVI to activate platelets (Fig. 6C) (11, 28) and by the ability of soluble collagens to support adhesion of GPVI-expressing RBL-2H3 cells (Fig. 6D). Thus, platelet responses to soluble collagens can be explained on the basis of a severe but not absolute loss of GPVI activation with retention of {alpha}2ß1 interaction, resulting in platelet responses that phenocopy those of GPVI-blocked, low-GPVI, and LAT-deficient platelets to fibrillar collagen. The use of a soluble-type collagen also provides a logical explanation for the results of a study of collagen signaling in platelets performed prior to the molecular identification of GPVI that demonstrated {alpha}2ß1-dependent signaling mediated by Syk and PLC{gamma}2 (18). A model of reciprocal signaling by GPVI and {alpha}2ß1 therefore provides an explanation for the collagen responses observed both with platelets from genetically engineered mice and wild-type platelets with different forms of collagen.

The development of a dual receptor system in which integrin and nonintegrin receptors respond reciprocally to a common ligand suggests that each receptor contributes a specialized component of the platelet response to exposed collagen. Since GPVI responses to collagen are highly dependent upon receptor density (6), it is possible that GPVI-collagen interactions regulate the primary, graded signaling responses to vessel injury, whereas {alpha}2ß1 mediates rapid secondary adhesive and signaling responses once a threshold level of GPVI-collagen interaction has been reached. Since platelet-collagen interactions are believed to participate in primary platelet adhesion and activation events in the setting of flowing blood, such a system might have evolved to allow platelets to respond differentially but with adequate speed to varying degrees of vascular disruption and collagen exposure. Further testing of this model will provide greater insight into the role of integrin signaling during platelet activation and facilitate the development of novel therapeutic agents designed to block or slow the platelet collagen response in the setting of vascular disease.


arrow
ACKNOWLEDGMENTS
 
We thank Cezary Marcinkiewicz for providing EMS16, Barry Coller for providing 6F1 antibody, Skip Brass and Ed Morrisey for thoughtful discussions and critical reading of the manuscript, Larry Samelson for LAT-deficient mice, and Ying Liu and Ke Lu for valuable assistance with mouse husbandry and genotyping.

This work was supported by grants from the NHLBI (H.C. and M.L.K.), the AHA (M.L.K.), and the W. W. Smith Charitable Trust (M.L.K.).


arrow
FOOTNOTES
 
* Corresponding author. Mailing address: Division of Cardiology, Department of Medicine, University of Pennsylvania, BRB II/III Rm. 952, 421 Curie Blvd., Philadelphia, PA 19104. Phone: (215) 898-9007. Fax: (215) 573-2094. E-mail: markkahn{at}mail.med.upenn.edu. Back


arrow
REFERENCES
 
    1
  1. Alon, R., P. D. Kassner, M. W. Carr, E. B. Finger, M. E. Hemler, and T. A. Springer. 1995. The integrin VLA-4 supports tethering and rolling in flow on VCAM-1. J. Cell Biol. 128:1243-1253.[Abstract/Free Full Text]
    2. 2
  2. Barnes, M. J., C. G. Knight, and R. W. Farndale. 1998. The collagen-platelet interaction. Curr. Opin. Hematol. 5:314-320.[Medline]
    3. 3
  3. Berlanga, O., R. Bobe, M. Becker, G. Murphy, M. Leduc, C. Bon, F. A. Barry, J. M. Gibbins, P. Garcia, J. Frampton, and S. P. Watson. 2000. Expression of the collagen receptor glycoprotein VI during megakaryocyte differentiation. Blood 96:2740-2745.[Abstract/Free Full Text]
    4. 4
  4. Burger, S. R., M. M. Zutter, S. Sturgill-Koszycki, and S. A. Santoro. 1992. Induced cell surface expression of functional {alpha}2ß1 integrin during megakaryocytic differentiation of K562 leukemic cells. Exp. Cell Res. 202:28-35.[CrossRef][Medline]
    5. 5
  5. Chacko, G. W., A. M. Duchemin, K. M. Coggeshall, J. M. Osborne, J. T. Brandt, and C. L. Anderson. 1994. Clustering of the platelet Fc gamma receptor induces noncovalent association with the tyrosine kinase p72syk. J. Biol. Chem. 269:32435-32440.[Abstract/Free Full Text]
    6. 6
  6. Chen, H., D. Locke, Y. Liu, C. Liu, and M. L. Kahn. 2002. The platelet receptor GPVI mediates both adhesion and signaling responses to collagen in a receptor density-dependent fashion. J. Biol. Chem. 277:3011-3019.[Abstract/Free Full Text]
    7. 7
  7. Chen, J., T. G. Diacovo, D. G. Grenache, S. A. Santoro, and M. M. Zutter. 2002. The {alpha}2 integrin subunit-deficient mouse: a multifaceted phenotype including defects of branching morphogenesis and hemostasis. Am. J. Pathol. 161:337-344.[Abstract/Free Full Text]
    8. 8
  8. Clemetson, J. M., J. Polgar, E. Magnenat, T. N. Wells, and K. J. Clemetson. 1999. The platelet collagen receptor glycoprotein VI is a member of the immunoglobulin superfamily closely related to Fc{alpha}R and the natural killer receptors. J. Biol. Chem. 274:29019-29024.[Abstract/Free Full Text]
    9. 9
  9. Coller, B. S., J. H. Beer, L. E. Scudder, and M. H. Steinberg. 1989. Collagen-platelet interactions: evidence for a direct interaction of collagen with platelet GPIa/IIa and an indirect interaction with platelet GPIIb/IIIa mediated by adhesive proteins. Blood 74:182-192.[Abstract/Free Full Text]
    10. 10
  10. Gao, J., K. E. Zoller, M. H. Ginsberg, J. S. Brugge, and S. J. Shattil. 1997. Regulation of the pp72syk protein tyrosine kinase by platelet integrin {alpha}IIbß3. EMBO J. 16:6414-6425.[CrossRef][Medline]
    11. 11
  11. Holtkotter, O., B. Nieswandt, N. Smyth, W. Muller, M. Hafner, V. Schulte, T. Krieg, and B. Eckes. 2002. Integrin {alpha}2-deficient mice develop normally, are fertile, but display partially defective platelet interaction with collagen. J. Biol. Chem. 277:10789-10794.[Abstract/Free Full Text]
    12. 12
  12. Inoue, O., K. Suzuki-Inoue, W. L. Dean, J. Frampton, and S. P. Watson. 2003. Integrin {alpha}2ß1 mediates outside-in regulation of platelet spreading on collagen through activation of Src kinases and PLC{gamma}2. J. Cell Biol. 160:769-780.[Abstract/Free Full Text]
    13. 13
  13. Jennings, L. K., S. M. Slack, C. D. Wall, and T. H. Mondoro. 1996. Immunological comparisons of integrin alpha IIb beta 3 (GPIIb-IIIa) expressed on platelets and human erythroleukemia cells: evidence for cell specific differences. Blood Cells Mol. Dis. 22:23-35.
    14. 14
  14. Judd, B. A., P. S. Myung, L. Leng, A. Obergfell, W. S. Pear, S. J. Shattil, and G. A. Koretzky. 2000. Hematopoietic reconstitution of SLP-76 corrects hemostasis and platelet signaling through {alpha}IIbß3 and collagen receptors. Proc. Natl. Acad. Sci. USA 97:12056-12061.[Abstract/Free Full Text]
    15. 15
  15. Judd, B. A., P. S. Myung, A. Obergfell, E. E. Myers, A. M. Cheng, S. P. Watson, W. S. Pear, D. Allman, S. J. Shattil, and G. A. Koretzky. 2002. Differential requirement for LAT and SLP-76 in GPVI versus T-cell receptor signaling. J. Exp. Med. 195:705-717.[Abstract/Free Full Text]
    16. 16
  16. Jung, S. M., and M. Moroi. 1998. Platelets interact with soluble and insoluble collagens through characteristically different reactions. J. Biol. Chem. 273:14827-14837.[Abstract/Free Full Text]
    17. 17
  17. Jung, S. M., and M. Moroi. 2000. Signal-transducing mechanisms involved in activation of the platelet collagen receptor integrin {alpha}2ß1. J. Biol. Chem. 275:8016-8026.[Abstract/Free Full Text]
    18. 18
  18. Keely, P. J., and L. V. Parise. 1996. The {alpha}2ß1 integrin is a necessary co-receptor for collagen-induced activation of Syk and the subsequent phosphorylation of phospholipase C{gamma}2 in platelets. J. Biol. Chem. 271:26668-26676.[Abstract/Free Full Text]
    19. 19
  19. Kehrel, B., L. Balleisen, R. Kokott, R. Mesters, W. Stenzinger, K. J. Clemetson, and J. van de Loo. 1988. Deficiency of intact thrombospondin and membrane glycoprotein Ia in platelets with defective collagen-induced aggregation and spontaneous loss of disorder. Blood 71:1074-1078.[Abstract/Free Full Text]
    20. 20
  20. Kimura, T., H. Kihara, S. Bhattacharyya, H. Sakamoto, E. Appella, and R. P. Siraganian. 1996. Downstream signaling molecules bind to different phosphorylated immunoreceptor tyrosine-based activation motif (ITAM) peptides of the high-affinity IgE receptor. J. Biol. Chem. 271:27962-27968.[Abstract/Free Full Text]
    21. 21
  21. Locke, D., H. Chen, Y. Liu, C. Liu, and M. L. Kahn. 2002. Lipid rafts orchestrate signaling by the platelet receptor glycoprotein VI. J. Biol. Chem. 277:18801-18809.[Abstract/Free Full Text]
    22. 22
  22. Lusis, A. J. 2000. Atherosclerosis. Nature 407:233-241.[CrossRef][Medline]
    23. 23
  23. Marcinkiewicz, C., R. R. Lobb, M. M. Marcinkiewicz, J. L. Daniel, J. B. Smith, C. Dangelmaier, P. H. Weinreb, D. A. Beacham, and S. Niewiarowski. 2000. Isolation and characterization of EMS16, a C-lectin type protein from Echis multisquamatus venom, a potent and selective inhibitor of the {alpha}2ß1 integrin. Biochemistry 39:9859-9867.[CrossRef][Medline]
    24. 24
  24. McKenzie, S. E., S. M. Taylor, P. Malladi, H. Yuhan, D. L. Cassel, P. Chien, E. Schwartz, A. D. Schreiber, S. Surrey, and M. P. Reilly. 1999. The role of the human Fc receptor Fc gamma RIIA in the immune clearance of platelets: a transgenic mouse model. J. Immunol. 162:4311-4318.[Abstract/Free Full Text]
    25. 25
  25. Mendrick, D. L., D. M. Kelly, S. S. duMont, and D. J. Sandstrom. 1995. Glomerular epithelial and mesangial cells differentially modulate the binding specificities of VLA-1 and VLA-2. Lab. Investig. 72:367-375.[Medline]
    26. 26
  26. Moroi, M., S. M. Jung, M. Okuma, and K. Shinmyozu. 1989. A patient with platelets deficient in glycoprotein VI that lack both collagen-induced aggregation and adhesion. J. Clin. Investig. 84:1440-1445.
    27. 27
  27. Nieswandt, B., W. Bergmeier, V. Schulte, K. Rackebrandt, J. E. Gessner, and H. Zirngibl. 2000. Expression and function of the mouse collagen receptor glycoprotein VI is strictly dependent on its association with the FcR{gamma} chain. J. Biol. Chem. 275:23998-24002.[Abstract/Free Full Text]
    28. 28
  28. Nieswandt, B., C. Brakebusch, W. Bergmeier, V. Schulte, D. Bouvard, R. Mokhtari-Nejad, T. Lindhout, J. W. Heemskerk, H. Zirngibl, and R. Fassler. 2001. Glycoprotein VI but not {alpha}2ß1 integrin is essential for platelet interaction with collagen. EMBO J. 20:2120-2130.[CrossRef][Medline]
    29. 29
  29. Nieswandt, B., V. Schulte, W. Bergmeier, R. Mokhtari-Nejad, K. Rackebrandt, J. P. Cazenave, P. Ohlmann, C. Gachet, and H. Zirngibl. 2001. Long-term antithrombotic protection by in vivo depletion of platelet glycoprotein VI in mice. J. Exp. Med. 193:459-469.[Abstract/Free Full Text]
    30. 30
  30. Nieuwenhuis, H. K., J. W. Akkerman, W. P. Houdijk, and J. J. Sixma. 1985. Human blood platelets showing no response to collagen fail to express surface glycoprotein Ia. Nature 318:470-472.[CrossRef][Medline]
    31. 31
  31. O'Toole, T. E., D. Mandelman, J. Forsyth, S. J. Shattil, E. F. Plow, and M. H. Ginsberg. 1991. Modulation of the affinity of integrin alpha IIb beta 3 (GPIIb-IIIa) by the cytoplasmic domain of alpha IIb. Science 254:845-847.[Abstract/Free Full Text]
    32. 32
  32. Obergfell, A., B. A. Judd, M. A. del Pozo, M. A. Schwartz, G. A. Koretzky, and S. J. Shattil. 2001. The molecular adapter SLP-76 relays signals from platelet integrin {alpha}IIbß3 to the actin cytoskeleton. J. Biol. Chem. 276:5916-5923.
    33. 33
  33. Pasquet, J. M., B. Gross, L. Quek, N. Asazuma, W. Zhang, C. L. Sommers, E. Schweighoffer, V. Tybulewicz, B. Judd, J. R. Lee, G. Koretzky, P. E. Love, L. E. Samelson, and S. P. Watson. 1999. LAT is required for tyrosine phosphorylation of phospholipase C{gamma}2 and platelet activation by the collagen receptor GPVI. Mol. Cell. Biol. 19:8326-8334.[Abstract/Free Full Text]
    34. 34
  34. Santoro, S. A. 1986. Identification of a 160,000 dalton platelet membrane protein that mediates the initial divalent cation-dependent adhesion of platelets to collagen. Cell 46:913-920.[CrossRef][Medline]
    35. 35
  35. Savage, B., M. H. Ginsberg, and Z. M. Ruggeri. 1999. Influence of fibrillar collagen structure on the mechanisms of platelet thrombus formation under flow. Blood 94:2704-2715.[Abstract/Free Full Text]
    36. 36
  36. Schulte, V., D. Snell, W. Bergmeier, H. Zirngibl, S. P. Watson, and B. Nieswandt. 2001. Evidence for two distinct epitopes within collagen for activation of murine platelets. J. Biol. Chem. 276:364-368.
    37. 37
  37. Snell, D. C., V. Schulte, G. E. Jarvis, K. Arase, D. Sakurai, T. Saito, S. P. Watson, and B. Nieswandt. 2002. Differential effects of reduced glycoprotein VI levels on activation of murine platelets by glycoprotein VI ligands. Biochem. J. 368:293-300.[CrossRef][Medline]
    38. 38
  38. Wilner, G. D., H. L. Nossel, and E. C. LeRoy. 1968. Aggregation of platelets by collagen. J. Clin. Investig. 47:2616-2621.
    39. 39
  39. Wonerow, P., A. Obergfell, J. I. Wilde, R. Bobe, N. Asazuma, T. Brdicka, A. Leo, B. Schraven, V. Horejsi, S. J. Shattil, and S. P. Watson. 2002. Differential role of glycolipid-enriched membrane domains in glycoprotein VI- and integrin-mediated phospholipase C{gamma}2 regulation in platelets. Biochem. J. 364:755-765.[CrossRef][Medline]
    40. 40
  40. Woodside, D. G., A. Obergfell, L. Leng, J. L. Wilsbacher, C. K. Miranti, J. S. Brugge, S. J. Shattil, and M. H. Ginsberg. 2001. Activation of Syk protein tyrosine kinase through interaction with integrin ß cytoplasmic domains. Curr. Biol. 11:1799-1804.[CrossRef][Medline]
    41. 41
  41. Zhang, W., R. P. Trible, and L. E. Samelson. 1998. LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T-cell activation. Immunity 9:239-246.[CrossRef][Medline]
    42. 42
  42. Zutter, M. M., S. A. Santoro, J. E. Wu, T. Wakatsuki, S. K. Dickeson, and E. L. Elson. 1999. Collagen receptor control of epithelial morphogenesis and cell cycle progression. Am. J. Pathol. 155:927-940.[Abstract/Free Full Text]

Molecular and Cellular Biology, July 2003, p. 4764-4777, Vol. 23, No. 14
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.14.4764-4777.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boylan, B., Gao, C., Rathore, V., Gill, J. C., Newman, D. K., Newman, P. J. (2008). Identification of Fc{gamma}RIIa as the ITAM-bearing receptor mediating {alpha}IIb{beta}3 outside-in integrin signaling in human platelets. Blood 112: 2780-2786 [Abstract] [Full Text]  
  • Bezman, N. A., Lian, L., Abrams, C. S., Brass, L. F., Kahn, M. L., Jordan, M. S., Koretzky, G. A. (2008). Requirements of SLP76 tyrosines in ITAM and integrin receptor signaling and in platelet function in vivo. JEM 205: 1775-1788 [Abstract] [Full Text]  
  • Varga-Szabo, D., Pleines, I., Nieswandt, B. (2008). Cell Adhesion Mechanisms in Platelets. Arterioscler. Thromb. Vasc. Bio. 28: 403-412 [Abstract] [Full Text]  
  • Calvo, E., Tokumasu, F., Marinotti, O., Villeval, J.-L., Ribeiro, J. M. C., Francischetti, I. M. B. (2007). Aegyptin, a Novel Mosquito Salivary Gland Protein, Specifically Binds to Collagen and Prevents Its Interaction with Platelet Glycoprotein VI, Integrin {alpha}2beta1, and von Willebrand Factor. J. Biol. Chem. 282: 26928-26938 [Abstract] [Full Text]  
  • Bix, G., Iozzo, R. A., Woodall, B., Burrows, M., McQuillan, A., Campbell, S., Fields, G. B., Iozzo, R. V. (2007). Endorepellin, the C-terminal angiostatic module of perlecan, enhances collagen-platelet responses via the {alpha}2{beta}1-integrin receptor. Blood 109: 3745-3748 [Abstract] [Full Text]  
  • Strehl, A., Munnix, I. C. A., Kuijpers, M. J. E., van der Meijden, P. E. J., Cosemans, J. M. E. M., Feijge, M. A. H., Nieswandt, B., Heemskerk, J. W. M. (2007). Dual Role of Platelet Protein Kinase C in Thrombus Formation: STIMULATION OF PRO-AGGREGATORY AND SUPPRESSION OF PROCOAGULANT ACTIVITY IN PLATELETS. J. Biol. Chem. 282: 7046-7055 [Abstract] [Full Text]  
  • Samaha, F. F., Kahn, M. L. (2006). Novel Platelet and Vascular Roles for Immunoreceptor Signaling. Arterioscler. Thromb. Vasc. Bio. 26: 2588-2593 [Abstract] [Full Text]  
  • Cosemans, J. M. E. M., Munnix, I. C. A., Wetzker, R., Heller, R., Jackson, S. P., Heemskerk, J. W. M. (2006). Continuous signaling via PI3K isoforms beta and {gamma} is required for platelet ADP receptor function in dynamic thrombus stabilization. Blood 108: 3045-3052 [Abstract] [Full Text]  
  • Abtahian, F., Bezman, N., Clemens, R., Sebzda, E., Cheng, L., Shattil, S. J., Kahn, M. L., Koretzky, G. A. (2006). Evidence for the Requirement of ITAM Domains but Not SLP-76/Gads Interaction for Integrin Signaling in Hematopoietic Cells.. Mol. Cell. Biol. 26: 6936-6949 [Abstract] [Full Text]  
  • Boylan, B., Berndt, M. C., Kahn, M. L., Newman, P. J. (2006). Activation-independent, antibody-mediated removal of GPVI from circulating human platelets: development of a novel NOD/SCID mouse model to evaluate the in vivo effectiveness of anti-human platelet agents. Blood 108: 908-914 [Abstract] [Full Text]  
  • Horii, K., Kahn, M. L., Herr, A. B. (2006). Structural basis for platelet collagen responses by the immune-type receptor glycoprotein VI. Blood 108: 936-942 [Abstract] [Full Text]  
  • Newman, P. J. (2006). New role for the GPVI/FcR{gamma} chain complex. Blood 107: 1248-1249 [Full Text]  
  • Sarratt, K. L., Chen, H., Zutter, M. M., Santoro, S. A., Hammer, D. A., Kahn, M. L. (2005). GPVI and {alpha}2{beta}1 play independent critical roles during platelet adhesion and aggregate formation to collagen under flow. Blood 106: 1268-1277 [Abstract] [Full Text]  
  • Ajzenberg, N., Berroeta, C., Philip, I., Grandchamp, B., Ducellier, P., Huart, V., Verpillat, P., Guillin, M.-C., Benessiano, J. (2005). Association of the -92C/G and 807C/T Polymorphisms of the {alpha}2 Subunit Gene With Human Platelets {alpha}2{beta}1 Receptor Density. Arterioscler. Thromb. Vasc. Bio. 25: 1756-1760 [Abstract] [Full Text]  
  • Gruner, S., Prostredna, M., Aktas, B., Moers, A., Schulte, V., Krieg, T., Offermanns, S., Eckes, B., Nieswandt, B. (2004). Anti-Glycoprotein VI Treatment Severely Compromises Hemostasis in Mice With Reduced {alpha}2{beta}1 Levels or Concomitant Aspirin Therapy. Circulation 110: 2946-2951 [Abstract] [Full Text]  
  • Graham, K. L., Zeng, W., Takada, Y., Jackson, D. C., Coulson, B. S. (2004). Effects on Rotavirus Cell Binding and Infection of Monomeric and Polymeric Peptides Containing {alpha}2{beta}1 and {alpha}x{beta}2 Integrin Ligand Sequences. J. Virol. 78: 11786-11797 [Abstract] [Full Text]  
  • Lecut, C., Schoolmeester, A., Kuijpers, M. J.E., Broers, J. L.V., van Zandvoort, M. A.M.J., Vanhoorelbeke, K., Deckmyn, H., Jandrot-Perrus, M., Heemskerk, J. W.M. (2004). Principal Role of Glycoprotein VI in {alpha}2{beta}1 and {alpha}IIb{beta}3 Activation During Collagen-Induced Thrombus Formation. Arterioscler. Thromb. Vasc. Bio. 24: 1727-1733 [Abstract] [Full Text]  
  • Kuijpers, M. J. E., Schulte, V., Oury, C., Lindhout, T., Broers, J., Hoylaerts, M. F., Nieswandt, B., Heemskerk, J. W. M. (2004). Facilitating roles of murine platelet glycoprotein Ib and {alpha}IIb{beta}3 in phosphatidylserine exposure during vWF-collagen-induced thrombus formation. J. Physiol. 558: 403-415 [Abstract] [Full Text]  
  • Bennett, J. S. (2004). Dotting the I of an I-domain. Blood 104: 299-300 [Full Text]  
  • Kahn, M. L. (2004). Platelet collagen receptors play molecular ping-pong. Blood 103: 1180-1181 [Full Text]  

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, H.
Right arrow Articles by Kahn, M. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, H.
Right arrow Articles by Kahn, M. L.

Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»