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Molecular and Cellular Biology, March 2003, p. 1581-1589, Vol. 23, No. 5
0270-7306/03/$08.00+0     DOI: 10.1128/MCB.23.5.1581-1589.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Distinct Mechanisms of Receptor and Nonreceptor Tyrosine Kinase Activation by Reactive Oxygen Species in Vascular Smooth Muscle Cells: Role of Metalloprotease and Protein Kinase C-

Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232,¹ Institute of Molecular Oncology, Showa University, Tokyo 142-8555,² Department of Biochemistry, Sapporo Medical University, Sapporo 060-8556,³ Department of Biochemistry, School of Allied Health Science, Osaka University Faculty of Medicine, Osaka 565-0871, Japan,⁴ Pacific Northwest Research Institute, Seattle, Washington 98122⁵

Received 24 July 2002/ Returned for modification 4 September 2002/ Accepted 2 December 2002

	ABSTRACT

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Reactive oxygen species (ROS) are implicated in cardiovascular diseases. ROS, such as H₂O₂, act as second messengers to activate diverse signaling pathways. Although H₂O₂ activates several tyrosine kinases, including the epidermal growth factor (EGF) receptor, JAK2, and PYK2, in vascular smooth muscle cells (VSMCs), the intracellular mechanism by which ROS activate these tyrosine kinases remains unclear. Here, we identified two distinct signaling pathways required for receptor and nonreceptor tyrosine kinase activation by H₂O₂ involving a metalloprotease-dependent generation of heparin-binding EGF-like growth factor (HB-EGF) and protein kinase C (PKC)- activation, respectively. H₂O₂-induced EGF receptor tyrosine phosphorylation was inhibited by a metalloprotease inhibitor, whereas the inhibitor had no effect on H₂O₂-induced JAK2 tyrosine phosphorylation. HB-EGF neutralizing antibody inhibited H₂O₂-induced EGF receptor phosphorylation. In COS-7 cells expressing an HB-EGF construct tagged with alkaline phosphatase, H₂O₂ stimulates HB-EGF production through metalloprotease activation. By contrast, dominant negative PKC- transfection inhibited H₂O₂-induced JAK2 phosphorylation but not EGF receptor phosphorylation. Dominant negative PYK2 inhibited H₂O₂-induced JAK2 activation but not EGF receptor activation, whereas dominant negative PKC- inhibited PYK2 activation by H₂O₂. These data demonstrate the presence of distinct tyrosine kinase activation pathways (PKC-/PYK2/JAK2 and metalloprotease/HB-EGF/EGF receptor) utilized by H₂O₂ in VSMCs, thus providing unique therapeutic targets for cardiovascular diseases.

	INTRODUCTION

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Reactive oxygen species (ROS), including superoxide anion and hydrogen peroxide (H₂O₂), are known to act as second messengers (21, 41). ROS activate a wide variety of serine-threonine and tyrosine kinases, which are key regulatory proteins of signal transduction pathways important in mediating cellular growth, apoptosis, survival, migration, and aging (22, 35). An increasing body of evidence suggests that ROS and tyrosine kinase play prominent roles in the development and progression of the cardiovascular remodeling associated with hypertension, atherosclerosis, and restenosis after balloon angioplasty (2, 29).

ROS activate nonreceptor tyrosine kinases JAK2 (1, 59), PYK2/CAKß (25), and Src (11, 67) and receptor tyrosine kinases epidermal growth factor (EGF) receptor (23, 53, 67) and platelet-derived growth factor receptor (34) in vascular smooth muscle cells (VSMCs) as well as other cell lines. A few studies have shown an inhibition of ligand-stimulated receptor tyrosine kinase induced by ROS (33), suggesting a dominant role for ROS as a tyrosine kinase activator. Among tyrosine kinases activated by ROS, the EGF receptor and JAK2 are of particular interest in VSMCs. A G-protein-coupled receptor (GPCR) agonist, angiotensin II (AngII), has been shown to utilize ROS to activate the EGF receptor in VSMCs (23, 66). The activation of the EGF receptor by AngII or thrombin appears to be required for extracellular signal-regulated kinase (ERK) activation and the subsequent growth of VSMCs (15, 17, 19, 38). By contrast, ROS-dependent JAK2 activation is required for AngII-induced cytokine induction (56) and thrombin-induced heat shock protein induction (47) in VSMCs. Thus, ROS-dependent activation of the EGF receptor and JAK2 could mediate two distinct functions in VSMCs, such as growth and inflammatory responses, respectively. However, whether ROS activate the EGF receptor and JAK2 through distinct mechanisms remains unknown.

Recently, it has become apparent that the EGF receptor is also a part of the signaling networks activated by stimuli that do not directly interact with this receptor. These stimuli include agonists that specifically bind to other membrane receptors and environmental stressors (6). Collectively, EGF receptor transactivation by these factors is employed in a wide array of biological signaling responses (32, 45), which may participate in several disease processes (4, 42, 50). In this regard, EGF receptor transactivation is a current topic of signal transduction research.

ROS have been proposed to exert their effects through targeting the cysteine regions of the active sites of tyrosine phosphatases, which in turn activates tyrosine kinases (21). In fact, H₂O₂ has been shown to inhibit the dephosphorylation of the EGF receptor through the inhibition of a tyrosine phosphatase (39). Protein kinase C- (PKC-) is also implicated in ROS-dependent activation of tyrosine kinases, such as c-Abl and Src. H₂O₂ stimulates binding between PKC- and c-Abl where the activation of c-Abl is dependent on PKC- activation (61). Interestingly, H₂O₂-induced activation of PKC- is reported to be independent from tyrosine phosphatase inhibition (68). Alternatively, ROS may activate a tyrosine kinase by generating growth factors, such as heparin-binding EGF-like growth factor (HB-EGF), through metalloprotease cleavage. ROS production and metalloprotease-dependent HB-EGF generation are implicated in EGF receptor transactivation initiated through several GPCRs (9, 52). Our group has shown that both mechanisms are indispensable for EGF receptor transactivation induced by AngII in VSMCs (14, 23). In addition, a metalloprotease, ADAM17 (TACE), was reported to require PKC- activation to generate HB-EGF (37).

In this study, we examined the hypothesis that the activation of receptor and nonreceptor protein tyrosine kinases by ROS utilizes distinct signal transduction mechanisms involving a metalloprotease or PKC-. We found that a metalloprotease-dependent shedding of HB-EGF is required for H₂O₂-induced EGF receptor transactivation but not for JAK2 activation. By contrast, PKC- is required for H₂O₂-induced JAK2 activation but not for EGF receptor transactivation. The activation of JAK2 but not of the EGF receptor also requires PYK2 activation. Taken together, our findings provide a unique example of two distinct signaling pathways that mediate ROS-dependent tyrosine kinase activation in vascular cells.

	MATERIALS AND METHODS

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Reagents. BB2116 was kindly provided by Helen Mills (British Biotech). CGS27023, GM6001, AG1478, and rottlerin were purchased from Calbiochem. H₂O₂, AngII, N-acetylcysteine, and poly[Glu⁸⁰-Tyr²⁰] were purchased from Sigma. Antibodies were purchased from the following sources: phospho-JAK2, phospho-EGF receptor, and phospho-PYK2, BioSource International; JAK2, Upstate Biotechnology; EGF receptor, PKC-, PKC-, and PKC-ß1, Santa Cruz Biotechnology; PYK2, Transduction Laboratories; and neutralizing human HB-EGF, R & D Systems.

Cell culture. VSMCs were prepared from thoracic aortas of Sprague-Dawley rats (18). Subcultured cells from passages 3 to 12 were used and showed 99% positive immunostaining with smooth muscle -actin antibody (Sigma). Human aortic VSMCs were obtained from Clonetics and subcultured according to the manufacturer's manual. For experiments, VSMCs at 80 to 90% confluency were used after serum depletion for 3 days.

Adenovirus transfection. The generation of kinase-inactive PKC-, PKC-, PKC-ß1, and PYK2/CAKß mutant-encoded adenovirus constructs is described in detail elsewhere (36, 48). VSMCs were infected with adenovirus for 2 days as previously described (17).

Immunoprecipitation and immunoblotting. After stimulation, cells were lysed with ice-cold immunoprecipitation buffer (150 mM NaCl, 20 mM Tris [pH 7.5], 1% Triton X-100, 5 mM EDTA, 50 mM NaF, 10% [vol/vol] glycerol, 10 mg of leupeptin, 10 µg of aprotinin, and 10 µg of phenylmethylsulfonyl fluoride). The lysates were centrifuged, and the supernatant was immunoprecipitated with antibody and protein A/G agarose at 4°C for 16 h (19). Cell or immunoprecipitation lysates were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to a nitrocellulose membrane, and immunoblotted as described previously (19).

HB-EGF shedding assay. To examine the release of soluble HB-EGF, COS-7 cells were transfected with the alkaline phosphatase (AP)-tagged HB-EGF (HB-EGF-AP) plasmid (63) by a transferrin receptor-operated transfer (8, 58) with TransFast transfection reagent (Promega). Forty-eight hours after transfection, the medium was changed to Dulbecco modified Eagle medium without phenol red and cells were stimulated with H₂O₂. HB-EGF-AP secreted into the medium was assessed by measuring AP activity (63).

PKC- kinase assay. Kinase activity of PKC- was measured by an immune complex kinase assay as described previously (26, 61). After stimulation, cells were lysed with a buffer containing 50 mM HEPES (pH 7.5), 0.5% NP-40, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM sodium orthovanadate, 1 mM dithiothreitol, 1 mM NaF, 2 mM phenylmethylsulfonyl fluoride, and 10 µg each of pepstatin and leupeptin/ml. Cell lysates were centrifuged, and the supernatant was immunoprecipitated with anti-PKC- antibody for 2.5 h. The kinase assay was performed with a kinase assay buffer (20 mM Tris-HCl [pH 7.5], 10 mM MgCl₂, 2.5 µCi of [-³²P]ATP, and a substrate, 200 µg of histone H1/ml) incubated for 15 min at 30°C.

PYK2 kinase assay. PYK2 kinase activity was measured by an immune complex kinase assay as described previously (25). In brief, the cell lysates were centrifuged and the supernatant was immunoprecipitated with anti-PYK2 antibody for 2.5 h at 4°C. After being washed, the immune complexes were incubated with or without H₂O₂ for 10 min at room temperature in the kinase buffer (100 mM sodium HEPES [pH 7.6], 60 mM MgCl₂, 2 mM MnCl₂, 0.2 mM Na₃VO₄, 0.2% Triton X-100). Afterwards, the lysates were incubated at room temperature in kinase buffer containing 0.25 mg of poly[Glu⁸⁰-Tyr²⁰] and 2.5 µCi of [-³²P]ATP for 15 min. The reaction mixture was spotted onto Whatman 3MM paper, washed, and then measured by liquid scintillation counting.

	RESULTS

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H₂O₂ stimulates EGF receptor activation and JAK2 activation. The activation of the EGF receptor by H₂O₂ was assessed by a phospho-specific antibody. This antibody selectively recognizes the EGF receptor only when Tyr¹⁰⁶⁸ (a Grb2 binding site) is autophosphorylated. Also, the activation of JAK2 was assessed by a phospho-specific antibody that selectively recognizes Tyr^1007/1008 dually phosphorylated JAK2. These tyrosine residues are believed to be autophosphorylation sites, with Tyr¹⁰⁰⁷ phosphorylation being essential for JAK2 kinase activity (57). The specificities of these antibodies have been established previously (23). As shown in Fig. 1A, H₂O₂ time-dependently stimulated the phosphorylation of the EGF receptor and JAK2, with maximal phosphorylation occurring at 10 min. As shown in Fig. 1B, H₂O₂ concentration-dependently stimulated the phosphorylation of the EGF receptor and JAK2, with maximal phosphorylation occurring at H₂O₂ concentrations of 2 to 20 µM. These data suggest that the EGF receptor and JAK2 represent ROS-sensitive tyrosine kinases in VSMCs.

View larger version (61K): [in this window] [in a new window]   FIG. 1. Activation of EGF receptor and JAK2 by H2O2 in VSMCs. Cells were stimulated with 20 µM H2O2 for the indicated time periods (A) or with various concentrations of H2O2 for 10 min (B). Cell lysates were immunoblotted by antibodies toward phospho-EGF receptor (EGFR-p), EGF receptor (EGFR), phospho-JAK2 (JAK2-p), and JAK2 as indicated. Numbers on the left are molecular weights.

View larger version (39K): [in this window] [in a new window]   FIG. 2. Effect of metalloprotease inhibitor on H2O2-induced activation of EGF receptor and JAK2. (A) VSMCs were pretreated with the indicated concentrations of BB2116 for 30 min and stimulated with H2O2 (20 µM) for 10 min. Cell lysates were immunoblotted by antibodies toward phospho-EGF receptor (EGFR-p), EGF receptor (EGFR), and phospho-JAK2 (JAK2-p). (B) Densitometric analysis of EGF receptor and JAK2 phosphorylation. VSMCs were pretreated with or without 20 µM BB2116 for 30 min and stimulated with H2O2 (20 µM) for 10 min. Results are the means ± standard errors of the means (SEM) (n = 4). Asterisk, P < 0.05 versus H2O2 stimulation.

View larger version (23K): [in this window] [in a new window]   FIG. 3. Involvement of HB-EGF in H2O2-induced EGF receptor activation. (A) Human VSMCs were pretreated with HB-EGF neutralizing antibody (HB-EGF; 40 µg/ml) for 1 h and stimulated with H2O2 (20 µM) for 10 min. Cell lysates were immunoblotted by phospho-EGF receptor antibody (EGFR-p) and EGF receptor (EGFR) antibody. Numbers on the left are molecular weights. (B) After pretreatment with or without BB2116 (20 µM) for 30 min, COS-7 cells transfected with HB-EGF-AP plasmid were stimulated with H2O2 (20 µM). AP activity in the medium was determined. Results are the means ± SEM (n = 3). OD, optical density; basal, control sample.

JAK2 activation but not EGF receptor activation requires PKC-.

View larger version (45K): [in this window] [in a new window]   FIG. 4. Effects of rottlerin, a PKC- inhibitor, on H2O2-induced JAK2 and EGF receptor activation. (A) VSMCs were pretreated with various concentrations of rottlerin as indicated for 30 min and stimulated with H2O2 (20 µM) for 10 min. Cell lysates were immunoblotted by antibody toward phospho-JAK2 (JAK2-p). (B) VSMCs were pretreated with rottlerin (10 µM) for 30 min and stimulated with H2O2 (20 µM) for 10 min. Cell lysates were immunoblotted by antibodies toward phospho-JAK2, phospho-EGF receptor (EGFR-p), and JAK2. (C) Densitometric analysis of EGF receptor (EGFR) and JAK2 phosphorylation in the immunoblot shown in Fig. 4B. Results are the means ± SEM (n = 4). (D) VSMCs were pretreated with 20 mM N-acetylcysteine (NAC) for 90 min (upper panels) or 10 µM rottlerin for 30 min (lower panels) as indicated and stimulated with AngII (100 nM) for 3 min. Cell lysates were immunoblotted by antibodies toward phospho-JAK2 and JAK2 or phospho-EGF receptor and EGF receptor as indicated. Basal, control sample.

View larger version (38K): [in this window] [in a new window]   FIG. 5. Effects of dominant negative PKC- transfection on H2O2-induced JAK2 and EGF receptor activation. (A) Cells were transfected with the indicated amount of adenovirus encoding dominant negative PKC- (DNPKC) for 48 h and stimulated by H2O2 (20 µM) for 10 min. The cell lysates were immunoblotted by antibodies toward phospho-JAK2 (JAK2-p), JAK2, phospho-EGF receptor (EGFR-p), EGF receptor (EGFR), and PKC-. (B) Densitometric analysis of EGF receptor and JAK2 phosphorylation. Cells were transfected with adenovirus encoding dominant negative PKC- (MOI, 100) and stimulated with H2O2 (20 µM) for 10 min. Results are the means ± SEM (n = 3). (C) Cells were transfected with dominant negative PKC- (DNPKC) or dominant negative PKC-ß1 (DNPKCß) for 48 h and stimulated by H2O2 (20 µM) for 10 min. The cell lysates were immunoblotted by antibodies toward phospho-JAK2, PKC-, or PKC-ß1.

View larger version (39K): [in this window] [in a new window]   FIG. 6. Involvement of PYK2 in H2O2-induced JAK2 activation. (A) Cells were transfected with adenovirus (MOI, 10) encoding dominant negative PYK2 (DNPYK2) for 48 h and stimulated by H2O2 (20 µM) for 10 min. The cell lysates were immunoblotted by antibodies toward phospho-JAK2 (JAK2-p), JAK2, phospho-EGF receptor (EGFR-p), EGF receptor (EGFR), and PYK2. (B) Cells were transfected with adenovirus (MOI, 100) encoding dominant negative PKC- (DNPKC) for 48 h and stimulated by H2O2 (20 µM) for 10 min. The cell lysates were immunoprecipitated with anti-PYK2 antibody and immunoblotted by antibodies toward phospho-PYK2 (PYK2-p) and PYK2. IP, immunoprecipitate.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

 
The significant finding reported in the present study is that ROS utilize distinct signaling mechanisms to mediate the activation of receptor and nonreceptor tyrosine kinases. Specifically, a metalloprotease-dependent cleavage of HB-EGF is required for H₂O₂-induced EGF receptor transactivation but not for JAK2 activation whereas PKC- is required for H₂O₂-induced PYK2/JAK2 activation and not for EGF receptor transactivation, as illustrated in Fig. 7. We believe that this is the first example showing that ROS activate tyrosine kinases through distinct mechanisms in the same cell culture system.

View larger version (29K): [in this window] [in a new window]   FIG. 7. Scheme illustrating proposed two distinct activation mechanisms of protein tyrosine kinases by H2O2 in VSMCs. EGFR, EGF receptor.

Alternatively, ROS may modulate intracellular signals such as c-Src and ERK, which may indirectly activate the metalloprotease responsible for HB-EGF generation. The contribution of the ERK cascade to HB-EGF and transforming growth factor- generation has been reported previously (20, 27, 65). However, the ERK cascade is unlikely to mediate HB-EGF generation by H₂O₂ in VSMCs. This is because the ERK cascade exists downstream of EGF receptor transactivation in H₂O₂-stimulated VSMCs (23). c-Src is involved in EGF receptor transactivation by GPCRs (3, 46, 66), and c-Src appears to exist upstream of HB-EGF release (51). Moreover, H₂O₂-induced EGF receptor transactivation was inhibited by a selective Src inhibitor, PP2, in endothelial cells (7). The role of c-Src in mediating HB-EGF-dependent EGF receptor transactivation by H₂O₂ is under current investigation.

Our findings presented here strongly suggest the requirement of PKC- for JAK2 activation by H₂O₂. In the present study, we have used rottlerin as a PKC- inhibitor because it is commonly used as a selective inhibitor of this PKC isoform. In fact, our group has shown that this inhibitor blocks the translocation of PKC- toward the membrane stimulated by AngII in VSMCs and that it also inhibits the autophosphorylation of human recombinant PKC- in vitro (26). In contrast, two recent publications reported a failure of PKC- inhibition by rottlerin in a kinase assay using a synthetic substrate and one of the publications reported that rottlerin showed additional inhibitory effects besides PKC- inhibition (10, 60). To further evaluate the involvement of PKC- in JAK2 activation by H₂O₂, we utilized kinase-inactive PKC mutants and showed that only the PKC- mutant inhibited JAK2 activation. This is in line with the recent finding that PKC- is required for JAK2 activation induced by AngII (26), a well-established ROS inducer (30), in VSMCs. In addition, several reports indicate that H₂O₂ stimulates PKC- activity in various cell types (40, 49). In this study, we also found that H₂O₂ could stimulate PKC- activity in VSMCs. In addition, PKC- was previously shown to be required for HB-EGF production, possibly through the activation of ADAM9 (37). However, our present findings rather eliminate the role of PKC- in H₂O₂-induced EGF receptor activation. This is in good agreement with previous findings by our group that PKC does not mediate EGF receptor transactivation induced by AngII (19).

VSMCs normally express PYK2 that is activated by ROS or AngII through ROS production (16, 25). It has been shown that PYK2 function is indispensable for several AngII-induced signaling pathways and subsequent hypertrophy in VSMCs (24, 54). Specifically, PYK2 is constitutively associated with JAK2 and is required for JAK2 activation by AngII (26). Although PYK2 is implicated in EGF receptor transactivation in fibroblasts (3), this may not be the case for EGF receptor transactivation in VSMCs (66). Here, we found that JAK2 activation but not EGF receptor activation by H₂O₂ requires PYK2, which appears to be downstream of PKC-. Interestingly, it was demonstrated that H₂O₂ stimulates PKC- and c-Abl association, where c-Abl is activated by a PKC--dependent mechanism (61). Thus, the possibility that JAK2 or PYK2 is a substrate with which PKC- is capable of associating should be considered. PYK2 and its related tyrosine kinase FAK share a common structure with conserved important motifs (43, 55). Recently, FAK was shown to be involved in AngII-induced growth-promoting responses in cultured VSMCs (28). Although our present findings together with previous findings showing an interaction between PYK2 and JAK2 in VSMCs strongly suggest a critical role for PYK2 in mediating ROS-dependent JAK2 activation, it is possible that dominant negative PYK2 may interfere with the FAK function together with PYK2 function. Therefore, further studies are needed to examine the role of FAK in JAK2 activation.

In the present study, we recognized that most approaches that interfered with H₂O₂-dependent activation did not result in complete inhibition. Thus, both EGF receptor activation and JAK2 activation by ROS could involve additional pathways independent from the pathways identified in this study. In this regard, Src family kinase-dependent pathways have been proposed to mediate JAK2 activation (1) or EGF receptor activation by ROS (7, 66). Also, ROS are believed to stimulate tyrosine phosphorylation by the inhibition of tyrosine phosphatases via the cysteine residues in the active site regions of these enzymes (21). Knebel et al. (39) in fact demonstrated that H₂O₂ could inhibit the dephosphorylation of the EGF receptor through the inhibition of tyrosine phosphatases. Thus, future research should be conducted to determine whether a tyrosine phosphatase or Src kinase is involved in one or both mechanisms of tyrosine kinase activation by ROS in VSMCs.

In conclusion, we have shown that ROS utilize distinct signal transduction mechanisms to activate nonreceptor and receptor protein tyrosine kinases in VSMCs. This important finding may lead to the selective inhibition of various distinct ROS functions that may sufficiently prevent or attenuate several cardiovascular-related diseases.

	ACKNOWLEDGMENTS

 
We thank Kunie Eguchi and Trinita Fitzgerald for their excellent technical assistance.

This work was supported by National Institute of Health training grant HL07323 and a United Negro College Fund/Merck postdoctoral science research fellowship (G. Frank), by an American Heart Association Scientist Development Grant and a Vanderbilt University Diabetes Center Pilot & Feasibility Proposal (S. Eguchi), and in part by the research grants HL58205, DK20593, and CA68485 from the National Institute of Health (T. Inagami).

	FOOTNOTES

 
* Corresponding author. Mailing address: Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146. Phone: (615) 322-4334. Fax: (615) 322-3201. E-mail: satoru.eguchi{at}vanderbilt.edu.

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