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Persistent Activation by Constitutive Ste7 Promotes Kss1-Mediated Invasive Growth but Fails To Support Fus3-Dependent Mating in Yeast
Mol. Cell. Biol. Maleri et al. 24: 9221

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Files in this Data Supplement:

  • Text, Figs. S1 - S4, Tables S1 and S2 - Supplemental Materials and Methods
    Figure S1. Phosphorylation site mapping of the Ste7 N terminal after in vitro phosphorylation by Fus3.
    Figure S2. Phosphorylation site mapping of the Ste7 catalytic and C-terminal domains after in vitro phosphorylation by Fus3.
    Figure S3. SDS-PAGE mobility of Ste7 phosphorylation site derivatives after pheromone induction in vivo.
    Figure S4. Micrographs comparing the morphology of invasive colonies from Fus3 and fus3∆ strains expressing different Ste7 phosphorylation site substitution mutations.
    Supplemental References
    Table S1. Plasmids.
    Table S2. Oligonucleotides.

    MS Word document, 727K





This Article
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