Files in this Data Supplement:

• Text, Figs. S1 - S4, Tables S1 and S2 - Supplemental Materials and Methods
  Figure S1. Phosphorylation site mapping of the Ste7 N terminal after in vitro phosphorylation by Fus3.
  Figure S2. Phosphorylation site mapping of the Ste7 catalytic and C-terminal domains after in vitro phosphorylation by Fus3.
  Figure S3. SDS-PAGE mobility of Ste7 phosphorylation site derivatives after pheromone induction in vivo.
  Figure S4. Micrographs comparing the morphology of invasive colonies from Fus3 and fus3∆ strains expressing different Ste7 phosphorylation site substitution mutations.
  Supplemental References
  Table S1. Plasmids.
  Table S2. Oligonucleotides.

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