
Counting of Rif1p and Rif2p on Saccharomyces cerevisiae Telomeres Regulates Telomere Length
Mol. Cell. Biol. Levy and Blackburn
24: 10857
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Files in this Data Supplement:
- Supplemental file 1
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Supplemental Table S1: Plasmids used in this study.
Supplemental Table S2: Oligonucleotides used in this study.
Supplementary Figure S1: Negative telomere length regulation by tethered Rif proteins and by PDZ-mediated protein interactions is not dependent of RAD52.
Supplementary Figure S2: Levels of rap1∆Cp are comparable to wild-type while rap1-17p levels are much reduced.
Supplementary Figure S3: Levels of Rap1-PDZp and Rap1∆C-PDZp are comparable to wild-type Pap1p levels.
Supplementary Figure S4: PDZ-mediated protein interactions at the telomere confer length regulation.
Other supplementary information:
Strain passaging on plates versus liquid culture and "jumpy" telomers.
rap1∆C versus rap1-17.
PAP1-PDX confers telomere length regulation in the absence of the Rif proteins.
Rap1p fusion proteins that do not lead to shorter telomeres.
Supplementary references.
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