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Molecular and Cellular Biology, February 2004, p. 1649-1654, Vol. 24, No. 4
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.4.1649-1654.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Capn5 Is Expressed in a Subset of T Cells and Is Dispensable for Development

Tanna Franz, Lara Winckler, Thomas Boehm, and T. Neil Dear*

Department of Developmental Immunology, Max Planck Institute for Immunobiology, D-79108 Freiburg, Germany

Received 25 September 2003/ Returned for modification 13 November 2003/ Accepted 19 November 2003


   ABSTRACT
Top
 Abstract
Introduction
 Materials and Methods
 Results and Discussion
 References
 
The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in embryonic and newborn thymuses, in various epithelial tissues, and in tissues of the central nervous system. In the thymus, Capn5 was expressed mainly in relatively immature CD25+ embryonic thymocytes. Despite the numerous expression sites of Capn5, the majority of Capn5-null mice were viable and fertile and appeared healthy. Histopathological analysis did not reveal any differences between Capn5-null and wild-type mice. There were no defects in the major T- or B-cell populations in the thymus, spleen, bone marrow, or peritoneum, nor did apoptosis appear abnormal in Capn5-null T cells. There was no evidence for the development of autoimmune disease in Capn5-null animals. However, a small proportion of homozygous null offspring from heterozygous matings were runted and most often did not survive to adulthood.


   INTRODUCTION
Top
 Abstract
 Introduction
Materials and Methods
 Results and Discussion
 References
 
Calpains are a family of cytosolic cysteine proteases. CAPN1 and CAPN2, which are dependent on micromolar and millimolar concentrations of Ca2+, respectively, for their in vitro proteolytic activity, consist of an isoform-specific large (80-kDa) subunit and an invariant small (30-kDa) subunit termed CAPN4 (17). The large-subunit protein is conveniently divided into four domains, I to IV. The crystal structure of CAPN2 (15, 37) reveals that catalytic domain II consists of two subdomains, IIa and IIb, which are reoriented upon Ca2+ binding to generate the protease active site. Domain III has some structural similarity to the C2 domain, which is known to bind Ca2+ (29). The crystal structure of the small subunit, which associates with domain IV, demonstrates the presence of five Ca2+-binding EF hand motifs in the C-terminal region of calpain (3, 21).

The calpain family consists of 13 different large-subunit genes (9). CAPN1, -2, -8, -9, -11, -12, and -14 conform to the classic four-domain structure described above (11, 12, 19, 34), whereas CAPN3 contains two large insertions (33). CAPN5, -6, -7, and -10 possess alternative C-terminal domains (4, 10, 13), and CAPN13 lacks domain IV altogether (9). In CAPN5, this domain, termed domain T, possesses homology to the C2 domain of phospholipases, as does domain N of CAPN7 (32; T. N. Dear and T. Boehm, unpublished data). Thus, these proteins, although lacking EF hand motifs, may still bind Ca2+. In addition, CAPN6 lacks amino acids that are considered to be essential for the cysteine protease active site (10).

Although a great deal of biochemical information on calpains has been accumulated, their physiological role is unclear. They function by limited cleavage of a variety of substrates (5) and have been implicated in a variety of processes, including apoptosis (38), cell division (24), modulation of integrin-cytoskeleton interactions (31), and synaptic plasticity (6). They have been associated with numerous pathological conditions, such as Alzheimer's disease, cataracts, demyelination, cardiac ischemia, inflammation, and traumatic brain injury (for reviews, see references 5, 35, and 39). In humans, mutations in CAPN3 are responsible for limb girdle muscular dystrophy type 2A (27). A single nucleotide polymorphism in intron 3 of CAPN10 is associated with type 2 diabetes mellitus (14).

Calpain activity is also associated with apoptosis in a variety of systems. In neurons, for example, similarities in substrate specificity and mechanisms of activation between calpains and caspases have been noted (38). The protein p25, which accumulates in the brains of patients with Alzheimer's disease and leads to neuronal apoptosis, is cleaved from p35 by both CAPN1 and CAPN2 (18, 20). Caspase 12, which is required for amyloid-ß neurotoxicity (26), can be activated by calpain (25).

Inactivation of specific calpain genes by homologous recombination is one potential method of gaining insight into calpain function. Disruption of mouse Capn4 results in embryonic lethality (1), while its inactivation in 3T3 cells leads to transformation and tumorigenesis (22). Disruption of Capn1 alters platelet function, but otherwise mice are viable and fertile (2). Inactivation of mouse Capn3 generates a phenotype similar to human limb girdle muscular dystrophy type 2A and results in apoptosis of skeletal myofibers possibly due to altered accumulation and nuclear translocation of I{kappa}B and NF-{kappa}B (28). We have used gene targeting in ES cells to inactivate the Capn5 gene.


   MATERIALS AND METHODS
Top
 Abstract
 Introduction
 Materials and Methods
Results and Discussion
 References
 
Mice. Capn5-null mice were maintained on a mixed 129/SvJ, C57BL/6J genetic background. For embryos, mice were mated in the evening and if a vaginal plug was observed the next morning, fertilization was assumed to have taken place at midnight.

Targeting strategy. Genomic clones for Capn5 were obtained by screening a 129/SvJ genomic DNA library constructed in the cosmid vector pSuperCos (Stratagene, La Jolla, Calif.). The genomic organization of Capn5 has been published previously (23). A 7-kb KpnI fragment encompassing exons 2 to 6 was subcloned into the vector pBluescript. An IRES-lacZ-MC1Neo cassette was inserted into the NaeI site located within exon 3, and a herpes simplex virus thymidine kinase cassette for negative selection was inserted downstream of the short arm of homology to create the targeting construct pNCL3KO-9.

Generation of Capn5-deficient mice. The targeting construct pNCL3KO-9 was linearized with NotI, and 25 µg was electroporated into 107 CJ7 ES cells. Selection proceeded in the presence of 400 µg of G418 per ml (active concentration) and 2 µM ganciclovir. After 9 days, colonies were picked, expanded, and analyzed by Southern blot analysis to identify targeted ES clones. The homologous targeting efficiency was 1 in 20. Positive clones were further analyzed to exclude any clones containing multiple insertions of the targeting vector. ES cells from two independent clones were injected into C57BL/6J blastocysts and largely agouti male chimeras were mated to C57BL/6J females. The resulting F1 offspring were screened by Southern blot analysis of tail biopsy genomic DNA for germ line transmission of the targeted allele and intercrossed to generate homozygous null mice.

Genotyping. DNA was isolated by tail biopsy, digested with EcoRV, separated by electrophoresis in a 0.8% (wt/vol) agarose gel, and transferred to Hybond-N nylon membrane. The membrane was hybridized to a 32P-labeled DNA probe corresponding to exon 7 of the Capn5 genomic sequence.

FACS analysis. Before staining, cell suspensions from all organs were passed through a 40-µm cell strainer to remove residual connective tissue and cell clumps. For each staining, 2 x 106 cells were transferred into a tube and centrifuged at 1,000 x g for 5 min. The supernatant was discarded, the cells were resuspended in 10 µl of primary antibody solution by vortexing, covered with aluminum foil to prevent bleaching and quenching, and incubated for 15 min on ice. To remove unbound antibody, 2 ml of fluorescence-activated cell sorter (FACS) buffer was added and the tube was centrifuged at 1,000 x g for 5 min. The supernatant was discarded, and the cells were resuspended in 200 µl of FACS buffer (3% [vol/vol] fetal calf serum, 0.15% [wt/vol] sodium azide in phosphate-buffered saline [PBS]). At least 200,000 cells within the lymphocyte live gate were acquired with a FACScalibur (Becton Dickinson). The data were collected and analyzed with CellQuest. The antibodies were labeled as follows:anti-CD3-allophycocyanin; anti-CD25-phycoerythrin; anti-CD44-Red670. All antibodies were obtained from Pharmingen and used at a dilution of 1:50.

ß-Galactosidase staining of tissues. Embryos or tissues were removed and fixed by incubating for 1 to 3 h in 4% (wt/vol) paraformaldehyde in PBS at 4°C with gentle shaking. The tissues were then washed briefly in PBS and incubated in staining solution (1 mg of 5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside [X-Gal] per ml, 5 mM crystalline potassium ferricyanide, 5 mM potassium ferricyanide trihydrate, and 2 mM MgCl2 in PBS) for up to 48 h. Staining was monitored and terminated at an appropriate time point by washing in PBS, followed by refixation for 16 h at 4°C.

Detection of ß-galactosidase-positive cells by FACS analysis. Embryonic thymus tissue was isolated and disaggregated by being pushed through a sieve and passed through a 40-µm cell strainer. A total of 200 µl of prewarmed staining solution (2 mM fluorescein di-ß-D-galactopyranoside [FDG; Molecular Probes, Eugene, Oreg.], 4% [vol/vol] fetal calf serum, and 10 mM HEPES [pH 7.2] in PBS) was added, and the solution was incubated for 2 min at 37°C. FDG loading was then terminated by addition of 1.8 ml of ice-cold PBS containing 4% (vol/vol) fetal calf serum, 10 mM HEPES (pH 7.2), and 1 µg of propidium iodide per ml. Cells were then stained for other cell surface markers for FACS analysis. FDG-positive cells were monitored by emission at 512 nm by using excitation at 488 nm.

Northern blot RNA analysis. Total RNA was isolated from mouse tissues by the guanidine isothiocyanate method (7). Ten micrograms of total RNA was separated by electrophoresis in a 1.4% (wt/vol) agarose gel containing 2.2 M formaldehyde as previously described (30) and blotted to Hybond-N nylon membrane (Amersham) in accordance with the manufacturer's instructions. The blot was hybridized to a 32P-labeled cDNA fragment corresponding to exons 7 to 15 of the Capn5 cDNA sequence in Expresshyb hybridization solution (Clontech). Hybridization and high-stringency washing conditions were performed in accordance with the manufacturer's instructions. The blot was reprobed with an Hprt cDNA probe to confirm RNA loading.


   RESULTS AND DISCUSSION
Top
 Abstract
 Introduction
 Materials and Methods
 Results and Discussion
References
 
Generation of Capn5-null mice. The Capn5 gene was disrupted by homologous recombination in ES cells. The targeting construct used contained an insertion of an IRES-lacZ-Neo cassette into exon 4 of Capn5 (Fig. 1A). This strategy was chosen because even if a partial protein was produced from the targeted allele, the protease active site was likely to be disrupted, as the critical cysteine of the active site lies upstream of the insertion while the asparagine and histidine lie downstream. Additionally, the inclusion of the IRES-lacZ insertion allows the expression of Capn5 in tissues to be examined by staining for ß-galactosidase activity. Chimeric mice were generated from these ES cells. Four mice were generated, two of which demonstrated germ line transmission. Intercrossing of mice heterozygous for the targeted mutation generated Capn5-deficient mice. To differentiate the wild-type and targeted alleles, a 32P-labeled probe corresponding to exon 7 and part of intron 6 was used, which detected 11.5- and 9.5-kb EcoRV fragments from the wild-type and targeted alleles, respectively. An example of a Southern blot analysis of DNAs from the weaned offspring of a mating between heterozygous mice is shown in Fig. 1B, indicating that mice homozygous for the targeted allele were viable.



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  		FIG. 1. Targeted disruption of the Capn5 gene. (A) The targeting construct was made by inserting an IRES-lacZ-Neo cassette into the NaeI restriction site in exon 4 of Capn5 genomic DNA. The linearized targeting construct and the endogenous genomic locus with relevant restriction enzyme sites are shown. The positions of the primers used for PCR analysis and the probe used for Southern blot analysis are indicated. (B) Southern blot analysis of EcoRV-digested DNAs from offspring of heterozygous matings hybridized with the 32P-labeled probe. The 11.5- and 9.5-kb bands generated from the wild-type and knockout alleles are indicated. (C) Northern blot analysis of total RNAs from the brains of wild-type mice and mice heterozygous and homozygous for the targeted Capn5 allele hybridized with a 32P-labeled Capn5 cDNA probe. The 2.3-kb Capn5 RNA transcript present in the wild-type and heterozygous samples is indicated. The blot was rehybridized with an Hprt cDNA probe to confirm that equivalent levels of RNA had been loaded. HSV-TK, herpes simplex virus thymidine kinase.

 
To determine whether the targeted allele was being transcribed, total RNAs from the brains of wild-type and heterozygous and homozygous null mice were examined by Northern blot assay for Capn5 RNA transcripts with a Capn5 cDNA probe located downstream of the IRES-lacZ insertion. Brain tissue was chosen for analysis as, compared to other adult tissues, it expresses high levels of Capn5 mRNA. There was no detectable Capn5 mRNA in the homozygous null animals (Fig. 1C). A similar absence of Capn5 mRNA was observed in liver RNA samples from homozygous null animals (data not shown). Thus, it appears that no complete Capn5 mRNA, and therefore no protein, can be produced in such mice.

Analysis of Capn5 expression in heterozygous knockout mice. The null allele contained an integrated lacZ gene such that ß-galactosidase is produced in cells normally expressing Capn5. This allowed a detailed examination of the expression pattern of Capn5 by staining of tissues from heterozygous mice for ß-galactosidase activity. By using in situ analysis, we previously found Capn5 mRNA to be expressed in the developing thymus and dorsal root ganglia (8). The Capn5lacZ allele was also expressed in these tissues (Fig. 2A and B), confirming that this allele was functional. lacZ staining was strongest in the thymus between embryonic days 13.5 and 19. Expression was weaker in postnatal thymuses and undetectable in thymuses of 4-week-old mice (data not shown). Other expression sites identified included the outer epithelium of the stomach (Fig. 2C and D), the pons nuclei and other nuclei of the brain (Fig. 2E), the bladder epithelium (Fig. 2F), and the ganglia of the gut (Fig. 2G and H). Additionally, staining was also observed in the mucosal epithelium of the nostrils and the whisker barrels (data not shown).



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  		FIG. 2. Expression of the Capn5lacZ allele during embryonic development. Postcoital day 14.5 embryos were removed and stained for ß-galactosidase (lacZ) activity. Tissues were then dissected and photographed. (A) Comparative expression in the thymuses of Capn5+/+ and Capn5+/- embryos. (B) Dorsal side of the neural tube of a Capn5+/- embryo showing expression in the dorsa root ganglia. (C) Expression in the epithelium of the stomach. (D) Histological section of the stomach stained for ß-galactosidase expression and counterstained with hematoxylin and eosin. Expression is restricted to the epithelial cells (arrow). (E) Expression in the brain in the pons nuclei and nuclei in the forebrain region. (F) Expression in the bladder epithelium (arrow). (G) Expression in ganglia of the intestine. (H) Close-up of the boxed region in panel G. Abbreviations: DRG, dorsal root ganglia; L, liver; H, heart; P, pons nuclei; Sp, spleen; St, stomach; T, thymus.

 
In situ hybridization of a 33P-labeled Capn5 RNA probe to tissue sections confirmed the above-mentioned sites expressing Capn5 mRNA. In addition, several new sites were also uncovered that were not detected by ß-galactosidase staining of heterozygous embryos, most likely because the expression was internal to the organ and thus masked in staining of whole organs. A common site was expression in epithelial cells. Expression was observed in the transitional epithelium of the afferent and efferent kidney tubules, the internal mucosa of the rectum, and the ependymal cells surrounding the central canal of the spinal cord, and weak expression was observed in the skin and the liver (data not shown). In adults, Capn5 mRNA had been down-regulated in these tissues; exceptions were the pons nuclei and two unidentified nuclei of the forebrain. In situ hybridization also detected expression in the pyramid cells of the dorsal and ventral hippocampus (data not shown).

Expression of Capn5 in T cells. As Capn5 is prominently expressed in the embryonic and newborn thymus, we examined expression in this organ in greater detail. Thymus cells from newborn mice heterozygous for the targeted Capn5lacZ allele were perfused with FDG, a substrate that is metabolized by ß-galactosidase to a fluorescent compound that can be detected by FACS analysis. The Capn5lacZ allele was expressed in about 15% of embryonic thymocytes with little expression in the stroma (Fig. 3A). FACS analysis was then performed with FDG in combination with various antibodies against thymocyte and stroma cell surface markers. None of the stromal subpopulations examined (dendritic cells, corticoepithelial cells, macrophages, and NK cells) expressed Capn5. In the thymocytes, there was no expression in the CD4, CD8, or {gamma}{delta}TCR population (data not shown). Approximately half of the CD3-positive cells expressed Capn5. A similar proportion of CD44-positive cells expressed Capn5 (Fig. 3B). All CD25+ thymocytes were ß-galactosidase positive, while CD25- thymocytes were negative (Fig. 3B). Thus, there is a strong correlation between the expression of CD25 and that of Capn5. This implies that Capn5 is expressed in immature thymocytes. Clearly, however, there is some expression in more mature cells as some CD3+ thymocytes expressed Capn5.



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  		FIG. 3. Analysis of Capn5lacZ allele expression in thymus cells. (A) Representative flow cytometric analysis of Capn5+/- thymocytes and stromal cells stained with FDG to detect ß-galactosidase activity. (B) Representative flow cytometric analysis of cells stained with FDG and antibodies against CD3, CD25, and CD44. Cells staining positively or negatively for CD3, CD25, or CD44 (top) were gated and analyzed for expression of ß-galactosidase (bottom).

 
Despite this, detailed FACS analysis of subpopulations in the thymus, spleen, lymph nodes, peritoneal cavity, and blood of Capn5-null mice and wild-type littermate controls did not reveal any differences in the proportions of T cells positive for a variety of hematopoietic cell markers, including CD25, CD44, CD3, CD4, CD8, CD62L, CD69, TCR{alpha}ß, and TCR{gamma}{delta} (data not shown). A detailed analysis of the major immune cell populations of the spleen, thymus, bone marrow, peritoneum, lymph nodes, and peripheral blood was undertaken, but no differences between wild-type and Capn5-null mice were detected. The response of Capn5-null mice to typical T-dependent and T-independent antigens was normal. As calpain activity has been associated with dexamethasone-induced apoptosis in thymocytes (36), such apoptosis was measured but no significant differences were observed between wild-type and Capn5-null thymocytes. Additionally, we tested whether Capn5-null mice were susceptible to induction of autoimmune diabetes by DNA vaccination with expression plasmids for insulin and GAD65 (16). Four-week-old mice were vaccinated at days 1 and 7 and observed for 4.5 months. No changes in blood glucose levels were observed between wild-type and Capn5-null mice. Furthermore, no other signs of autoimmunity, such as lymphocytic infiltrations in the salivary glands, adrenal glands, or liver, were observed.

Capn5-null mice exhibit reduced viability. Capn5-null mice appeared normal. There were no obvious abnormalities in behavior, morphology, or development. There were no differences in ß-galactosidase staining in heterozygous versus nullizygous embryos, suggesting that no major cell population is lost. We compared the distribution of genotypes in newborn litters of heterozygous matings. Examination of 17 litters (n = 120 pups) of Capn5+/- x Capn5+/- matings showed that the distribution of genotypes conformed to Mendelian expectations, with 37 (31%) Capn5+/+, 54 (45%) Capn5+/-, and 29 (24%) Capn5-/- mice (P = 0.3). It was notable, however, that a small number of pups from heterozygous matings (18 out of 418 pups = 4.3%) were severely runted at birth and most died by 3 weeks of age with a few surviving to 7 or 8 weeks of age (data not shown). DNA was obtained from nine of these runts, and all but one were homozygous for the Capn5 null mutation; the other mouse was heterozygous. As homozygous Capn5-null mice were viable and fertile, matings were established between homozygous null animals. The proportion of runted offspring increased to 9.6% in such matings (10 out of 104 pups). As all offspring from such matings were homozygous for the null mutation, this increase in runted offspring further suggested that homozygosity for the Capn5-null mutation contributed to the runting observed. Of the 10 runted mice obtained from these matings, 1 male and 1 female mouse survived to adulthood and although runted appeared otherwise fit. No offspring were obtained from the male, but the female was fertile and Capn5-/- pups obtained from this female appeared healthy, were of a normal size, and grew at a normal rate. The mutation was bred into the C57BL/6J strain by six generations of backcrossing. When heterozygous animals in this background were intercrossed, no runted pups were observed among homozygous offspring.

Conclusions. In summary, Capn5-deficient mice do not suffer from striking abnormalities affecting any of the known expression sites. The genetic background appears to influence the phenotype, with runting and premature death resulting in a small proportion of animals. The calpain gene family is large, with possibly 13 large-subunit isoforms (9), and it may be that functional redundancy accounts for the lack of a phenotype in Capn5-null mice. Characterization of mutant mice carrying various combinations of calpain knockout alleles may assist in determining the role of each gene. Alternatively, the changes in Capn5-deficient mice may be subtle, affecting processes that have not yet been investigated, or may only be manifested upon a specific challenge. Furthermore, as the targeted modification of the Capn5 gene results in a truncated protein, the possibility cannot be excluded that this protein retains some functional activity of sorts, thus acting as a hypomorphic rather than a nullimorphic allele.


   ACKNOWLEDGMENTS
 
This work was partially supported by a grant from BASF, Ludwigshafen, Germany.

We thank Benoit Kanzler for blastocyst injection of ES cells and Melanie Hunn for technical assistance.


   FOOTNOTES
 
* Corresponding author. Mailing address: Mary Lyon Centre, Medical Research Council, Harwell, Didcot OX11 0RD, United Kingdom. Phone: 44 1235 841104. Fax: 44 1235 841200. E-mail: n.dear{at}har.mrc.ac.uk. Back


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 Materials and Methods
 Results and Discussion
 References
 
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Molecular and Cellular Biology, February 2004, p. 1649-1654, Vol. 24, No. 4
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.4.1649-1654.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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