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Molecular and Cellular Biology, March 2004, p. 2074-2082, Vol. 24, No. 5
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.5.2074-2082.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Defective Neural Tube Closure and Anteroposterior Patterning in Mice Lacking the LIM Protein LMO4 or Its Interacting Partner Deaf-1

Kyungmin Hahm,^1,2, Eleanor Y. M. Sum,³ Yuko Fujiwara,¹ Geoffrey J. Lindeman,³ Jane E. Visvader,³ and Stuart H. Orkin^1,2*

Division of Hematology-Oncology, Department of Pediatrics, Children's Hospital and Dana-Farber Cancer Institute,¹ Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115,² VBCRC Laboratory, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia³

Received 10 November 2003/ Accepted 13 November 2003

	ABSTRACT

Top Abstract Introduction Materials and Methods Results Discussion References

 
LMO4 belongs to a family of transcriptional regulators that comprises two zinc-binding LIM domains. LIM-only (LMO) proteins appear to function as docking sites for other factors, leading to the assembly of multiprotein complexes. The transcription factor Deaf-1/NUDR has been identified as one partner protein of LMO4. We have disrupted the Lmo4 and Deaf-1 genes in mice to define their biological function in vivo. All Lmo4 mutants died shortly after birth and showed defects within the presphenoid bone, with 50% of mice also exhibiting exencephaly. Homeotic transformations were observed in Lmo4-null embryos and newborn mice, but with incomplete penetrance. These included skeletal defects in cervical vertebrae and the rib cage. Furthermore, fusions of cranial nerves IX and X and defects in cranial nerve V were apparent in some Lmo4^-/- and Lmo4^+/- mice. Remarkably, Deaf-1 mutants displayed phenotypic abnormalities similar to those observed in Lmo4 mutants. These included exencephaly, transformation of cervical segments, and rib cage abnormalities. In contrast to Lmo4 nullizygous mice, nonexencephalic Deaf-1 mutants remained healthy. No defects in the sphenoid bone or cranial nerves were apparent. Thus, Lmo4 and Deaf-1 mutant mice exhibit overlapping as well as distinct phenotypes. Our data indicate an important role for these two transcriptional regulators in pathways affecting neural tube closure and skeletal patterning, most likely reflecting their presence in a functional complex in vivo.

	INTRODUCTION

Top Abstract Introduction Materials and Methods Results Discussion References

 
The LIM domain is characterized by a double zinc finger structure and is found in proteins that have critical functions in cell fate determination, differentiation, and cytoskeleton organization (reviewed in references 2, 8, and 20). This motif was originally identified in LIM homeodomain transcription factors which have established roles within the central nervous system (CNS). The LIM domain also occurs in a variety of nuclear and cytoplasmic proteins, including LIM-only (LMO), LIM kinase, and focal adhesion proteins. In these proteins, there are usually two or more LIM domains, which may occur by themselves or in association with functionally divergent domains. One of the central functions of the LIM domain is to mediate protein-protein interactions, which may have either positive or negative effects on gene transcription (2, 20).

The LMO subclass of LIM proteins comprises four members (LMO1 to LMO4), each of which is defined by two tandem zinc finger domains (30). The LMO1 and LMO2 genes were originally identified by their translocation in acute T-cell leukemia, and their overexpression in transgenic mice leads to T-cell tumors (30). Lmo2 has been established to have a critical function in early hemopoiesis (44) and angiogenesis (43). Little is known about the physiological role of LMO3, which was cloned on the basis of sequence homology. LMO4 was identified by virtue of its interaction with the ubiquitous cofactor protein Ldb1/NLI/CLIM2 (13, 21, 33) and in an expression screen using autologous serum from a breast cancer patient (21, 31). It is the most divergent member of the family and is widely expressed in both embryonic and adult tissues, including thymus, skin, and distinct regions within the brain (6, 21, 33). The Lmo4 gene is also highly expressed in the proliferating mammary gland and is overexpressed in more than 50% of primary breast cancers (41), underscoring its importance in the regulation of cell growth.

LMO proteins appear to function as molecular adaptors for the assembly of multiprotein complexes (30). There is no evidence that this family of LIM proteins can bind DNA specifically, but rather, their functions are primarily mediated by protein-protein interaction. LMO proteins potentially modulate transcription by binding to transcription factors or chromatin modeling proteins. LMO2 has been established to form a complex comprising the hematopoietic transcription factors SCL(TAL-1)/E2A and GATA1 as well as the cofactor Ldb1 (42). Similarly, LMO4 has been shown to participate in a novel complex comprising BRCA1 and CtIP in breast epithelial cells (34). LMO4 also associates with other proteins, including the cofactor Ldb1 (13, 21), and the transcription factors Deformed Epidermal Autoregulatory Factor-1/Nuclear Deaf Related factor (DEAF-1/NUDR/Suppressin) (33), Grainyhead-like epithelial transactivator (GET-1) (22) and the basic helix-loop-helix protein HEN1 (24). DEAF-1/NUDR is a nuclear DNA-binding protein that was first shown to recognize sites within the autoregulatory element of the deformed gene in Drosophila melanogaster (12). DEAF-1 comprises two conserved domains (17)—SAND (Sp100, AIRE-1, NucP41/75, DEAF-1) (4, 11) and MYND (myeloid, nervy, and deaf-1) (12), both of which are found in several transcription factors. Similar to LMO4, DEAF-1 appears to be expressed widely (17, 23). Thus, DEAF-1 and LMO4 may act as general regulators of gene transcription and may function in concert to influence biological processes in specific cell types.

To further understand the biological roles of Lmo4 and Deaf-1, we have disrupted each gene by homologous recombination. We report here that mice lacking Lmo4 die perinatally from complex phenotypic abnormalities, with approximately 50% of mice exhibiting exencephaly. Lmo4-deficient mice displayed defects in their presphenoid bone and cranial nerves and homeotic transformations of their cervical vertebrae and rib cage. Strikingly, Deaf-1-deficient mice also displayed exencephaly, skeletal anomalies, and a low frequency of homeotic transformations but no presphenoid bone or cranial nerve defects. In contrast to Lmo4-null mice, Deaf-1 homozygotes that did not exhibit exencephaly survived the neonatal period and were essentially normal. Thus, Lmo4 and Deaf-1-null mice exhibit both distinct and common phenotypes. The overlapping phenotypes observed in these mutant mice suggest that LMO4 and DEAF-1 form a physiological complex in specific cell types.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

 
Targeted disruption of the murine Lmo4 locus. Lmo4 genomic clones were isolated from a FixII mouse strain 129 library (Stratagene). To generate a conditional targeting construct, a 1.0-kb HindIII fragment containing Lmo4 5' DNA and a 6.0-kb HindIII-ClaI fragment containing 3' DNA sequences were cloned into pTKLNCL (37). The 6.0-kb HindIII-ClaI fragment contains a loxP site inserted in the EcoRI site located 1.3 kb 3' of the HindIII site. The targeting construct was linearized with KpnI and electroporated into CJ-7 embryonic stem (ES) cells. Correctly targeted ES cell clones were injected into C57BL/6 blastocysts. To generate the Lmo4:LacZ knockin targeting construct, a 6.9-kb 5' fragment was isolated; this consists of a 4.6-kb BamHI-NcoI fragment containing the Lmo4 5' flanking genomic DNA plus a 2.3-kb fragment containing the LacZ coding sequence followed by a poly(A) signal. This 6.9-kb fragment and a 4.7-kb EcoRI-ClaI fragment containing the Lmo4 3' DNA were cloned into pTKLN, which lacks the PGK-cytosine deaminase cassette present in pTKLNCL. The targeting construct was linearized with SalI and electroporated into CJ-7 ES cells. One of three targeted ES cell clones injected into C57BL/6 blastocysts gave germ line transmission. Genotyping was performed by Southern blot analysis using a 0.6-kb XhoI-HindIII fragment on genomic DNA digested with XhoI and EcoRV.

Targeted disruption of the murine Deaf-1 locus. Deaf-1 genomic clones were isolated from a RPCI-22 mouse BAC library (Research Genetics). To generate a targeting construct, a 2.8-kb BamHI-Asp718 fragment containing Deaf-1 5' DNA and a 4.1-kb Asp718-XhoI fragment containing 3' DNA were cloned into pTKLN. The targeting construct was linearized with SalI and electroporated into CJ-7 ES cells. Two independently targeted ES cell clones were injected into C57BL/6 blastocysts, one of which gave germ line transmission. Genotyping was done by Southern blot analysis using a 1.2-kb BglII-EcoRV fragment to probe BglII-digested genomic DNA.

Mouse breeding and embryological techniques. Chimeras were crossed with CD-1 females carrying the Gata1-cre transgene (18, 25) or C57BL/6 females to obtain F₁ progeny carrying the targeted Lmo4 locus and/or GATA1-cre transgene. All analyses were performed with progeny from F₃ or subsequent generations. For neural crest-specific deletions, Lmo4^fl/fl mice carrying the Wnt1-cre transgene were generated by mating Lmo4^fl/fl mice with Wnt1-cre transgenic mice.

Whole-mount immunohistochemistry was performed on embryonic day 9.5 (E9.5) embryos using the antineurofilament antibody 2H3 (Developmental Hybridoma Bank, National Institute of Child Health and Human Development) as described by Swiatek and Gridley (35). Preparation of skeletons was as described previously (15). Briefly, embryos and mice were eviscerated, skinned, fixed in ethanol, and stained with Alcian blue or alizarin red (Sigma). Staining of embryos for lacZ expression was performed as described previously (25).

Immunoblot analysis. Protein lysates from wild-type and Lmo4-deficient E16.5 embryos were prepared in 1 ml of ice-cold lysis buffer (150 mM NaCl; 5 mM EDTA; 50 mM Tris-HCl [pH 7.5]; 1% NP-40 and 1 mM dithiothreitol, supplemented with Complete inhibitor tablet [Roche Diagnostics]; 10 mM NaF; 1 mM Na₃VO₄) from frozen embryos using a mortar and pestle. Total protein (30 µg) was denatured by boiling in sodium dodecyl sulfate loading buffer and then separated on polyacrylamide gels (Novex) prior to being transferred to polyvinylidene difluoride membranes (Millipore). Nonspecific binding of proteins to membranes was blocked by incubation in phosphate-buffered saline containing 5% skim milk and 0.1% Tween 20. The membranes were then probed with rat anti-LMO4 monoclonal antibody (1 to 2 µg/ml) or Deaf polyclonal rabbit antisera (17), a generous gift from J. Huggenvik. The membranes were subsequently incubated with horseradish peroxidase-coupled secondary antibodies (Dako) and developed by enhanced chemiluminescence (Amersham Biosciences, Inc.). To control for the integrity of proteins in tissue lysates, blots were reprobed with antitubulin monoclonal antibody (Sigma).

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

 
Generation of targeted Lmo4 and Lmo4:LacZ knockin mice. To investigate the role of Lmo4 during development, we used homologous recombination in ES cells to disrupt the murine Lmo4 locus (38), which comprises three primary coding exons. We constructed a targeting vector containing three loxP sites flanking the first coding exon of Lmo4 (exon 2) and the neomycin resistance cassette (Fig. 1A). Three clonal G418^R-targeted ES cell lines were produced, and appropriate integration was verified by Southern blot analysis using multiple probes flanking the targeted region. Chimeric mice generated from one ES cell line gave germ line transmission. These chimeras were crossed with GATA1-cre transgenic mice (18, 25), in which cre recombinase is expressed during early embryogenesis, to generate two types of mice: (i) mice in which only the neomycin resistance cassette in the Lmo4 locus was excised (floxed allele) and (ii) mice in which both the neomycin cassette and exon 2 of the Lmo4 gene were excised, yielding a knockout allele (Fig. 1C). Heterozygous mice were intercrossed to generate homozygous mutants. To verify that deletion of exon 2 (LIM1 domain) within the Lmo4 locus gave rise to animals null for Lmo4, we performed Western blot analysis using a rat anti-LMO4 monoclonal antibody that specifically recognizes the second LIM domain of LMO4. LMO4 protein was readily detectable in E16.5 Lmo4^+/+ embryos but not in Lmo4^-/- embryos (Fig. 1E). Thus, targeted disruption of the murine Lmo4 gene generated a null mutation.

View larger version (24K): [in this window] [in a new window]   FIG. 1. Targeted disruption of the mouse Lmo4 gene. (A) Partial restriction map of the mouse Lmo4 gene (top) and structure of the Lmo4 targeting vector (bottom), which contains the thymidine kinase, cytosine deaminase, and neomycin resistance (neor) genes, all under the control of the mouse PGK promoter. The location of the three loxP sites is given. Homologous recombination results in disruption of the coding sequence of Lmo4 and removal of exon 2, which encodes the first LIM domain. The filled boxes represent coding exons. The flanking probe used for Southern blot analysis is shown as a black bar. (B) Structure of the Lmo4:LacZ knockin construct. The construct contains the neor and thymidine kinase genes under the control of the mouse PGK promoter. Homologous recombination results in removal of the majority of exon 2 and disruption of the coding sequence of Lmo4. The LacZ gene (shaded) was fused in frame with the translation initiation codon located at the beginning of exon 2. Exons are indicated as filled boxes. Abbreviations for restriction sites: B, BamHI; RI, EcoRI; H, HindIII; X, XhoI; C, ClaI. (C) Generation of mice bearing either a constitutive null Lmo4 allele or a floxed allele. Chimeras were crossed with Gata1-cre transgenic females to obtain F1 progeny carrying either the floxed Lmo4 or knockout allele. Wild-type (11-kb), knockout (10-kb; both the neor cassette and floxed alleles excised), floxed (1.6-kb; only the neo cassette excised), and wild-type but modified (2.8-kb; targeted but neor cassette present) alleles are indicated. Tail DNA was digested with XhoI and EcoRV, and Southern blot analysis of F2 progeny was performed using a 600-bp XhoI-HindIII probe (A). (D) Lmo4:LacZ knockin mice were generated by breeding chimeras with Gata1-cre transgenic mice. Southern blot analysis of F1 progeny was performed using DNA digested with EcoRV and XhoI and a 600-bp XhoI-HindIII probe. (E) Protein lysates (30 µg) from either Lmo4-/- or wild-type embryos at E16.5 were analyzed by Western blotting using a monoclonal antibody specific for the second LIM domain of Lmo4. No truncated Lmo4 protein was detected in Lmo4-/- embryos. Immunoblotting with an antitubulin antibody (-tubulin) confirmed equal loading of protein.

Targeted disruption of the Lmo4 gene leads to perinatal lethality and defects in neural tube closure. Although progeny were born with the expected Mendelian ratio, no Lmo4^-/- neonates survived beyond the first day of birth. Among 193 live-born neonates, 45 corresponded to Lmo4^-/- mice, and 23 of these were found to have exencephaly. This phenomenon results from failure of neural tube closure in the mid- and hindbrain regions during early embryogenesis (Fig. 2A to C). The majority of defects in neural tube closure affected both the mid- and hindbrain regions of Lmo4-null mice (Fig. 2B and C). In some cases, malformation of the hindbrain was more prominent (data not shown). Exencephaly in the Lmo4 mutants was markedly reduced (less than 10%) on a C57BL/6 background, relative to that on a mixed (C57BL/6, CD-1, 129) background, consistent with previous findings that the exencephalic phenotype shows strain dependence (32).

View larger version (74K): [in this window] [in a new window]   FIG. 2. Failure of neural tube closure in Lmo4 mutants. Exencephaly was observed in approximately 50% of Lmo4 mutants. (A to C) Wild-type (A) and mutant Lmo4-/- (B and C) embryos at E9.0 with exencephaly. (D to F) Lmo4 expression in embryos at E8.5 (D and E) and E9.5 (F), as detected by LacZ staining of Lmo4:LacZ knockin embryos.

To address whether Lmo4 was expressed in the mid- and hindbrain regions during neural tube closure, Lmo4:LacZ knockin embryos were examined between E8.5 and E9.5. These mice accurately reflect the activity of the endogenous locus, as verified by immunohistochemistry using monoclonal LMO4 antibodies (unpublished data) and by RNA in situ hybridization (data not shown). In the developing mouse, the neural tube closes between E8.5 and E9.5 and is complete by late E9.5. Strong LacZ staining was evident in the mid- to hindbrain regions of E8.5 embryos as well as in the somites (Fig. 2D and E). In E9.5 embryos, prominent Lmo4 expression was also observed in the brain, branchial arches, and somites (Fig. 2F), as previously reported (21, 33). Thus, Lmo4 is expressed in the mid- and hindbrain regions at E8.5 and E9.5 when neural tube closure occurs, consistent with the observed phenotype.

Malformation of the presphenoid bone in Lmo4^-/- mice. Lmo4^-/- mice exhibited exencephaly with approximately 50% penetrance. In the remaining 50% of mutants, no gross defects in head structures were evident. To further investigate other potential abnormalities within the head region of both types of mutants, we examined the integrity of the skull by staining cartilage and bone. Exencephalic Lmo4^-/- neonates displayed a profoundly malformed sphenoid bone, in which the presphenoid body was missing and the trabecula basal plate that joins the pre- and basi-sphenoid was largely missing (Fig. 3F versus D). The presphenoid body gives rise to a part of the sphenoid bone which forms the central basal plate of the skull. The presphenoid bone is first observed in the mouse embryo at approximately E15.5, as cartilage with lateral processes protruding from the posterior end of the body (Fig. 3G). By E18.5, the presphenoid body is fully ossified (Fig. 3D). In nonexencephalic Lmo4 mutants, although the body of the presphenoid bone was present, the lateral processes originating from the posterior part of this body were missing in all embryos (eight out of eight) (Fig. 3G versus H). Examination of the presphenoid body in E15.5 to E17.5 Lmo4^-/- embryos demonstrated that these processes did not appear as cartilage primordia at E16.5 (Fig. 3H) and had failed to form by E17.5 (data not shown). Thus, malformation of the presphenoid bone in these Lmo4 mutants is likely to stem from improper formation of cartilage at E16.5. In normal animals, the cartilage within these lateral processes undergoes ossification and eventually contributes to the lateral walls of the optic canals, through which the optic fibers pass. In Lmo4 mutants (both E18.5 embryos and newborn mice), the inferior half of the lateral optic canal did not form properly and muscle and ligamentous attachments in this region were disorganized (data not shown).

View larger version (98K): [in this window] [in a new window]   FIG. 3. Malformation of the presphenoid bone in Lmo4 mutants. (A to F) Comparison of the internal skull base (ventral view) of wild-type mice (A and D) and newborn Lmo4 mutants without exencephaly (B and E) and with exencephaly (C and F), following staining for bone and cartilage using alizarin red and Alcian blue. The region surrounding the sphenoid bone is shown at higher magnification in D, E, and F. The presphenoid (PS) and the basi-sphenoid (BS) bodies are fully ossified (red stain). (D) The arrow depicts the lateroposterior processes protruding from the presphenoid body; these are missing in panels E and F. (G and H) Skull bases of E16.5 wild-type and Lmo4-/- embryos without exencephaly, respectively. Arrows point to the region where the lateroposterior processes lie. These are missing in panel H. (I to K) Skull bases of wild-type (I), Lmo4:LacZ knockin heterozygote (J), and ROSA26:LacZ:Wnt1-cre (K) embryos at E18.5 were stained for LacZ activity (ventral view). The lateroposterior processes present in panel J are missing in panel K. The blue staining in panel K, in the area where the processes lie, reflects background staining from the underlying tissue. ROSA26:LacZ:Wnt1-cre reporter embryos were generated by crossing Rosa26:LacZ reporter mice with Wnt1-cre transgenic mice. (L) Alizarin red and Alcian blue staining of bone and cartilage in the presphenoid bone in Lmo4fl/fl:Wnt1-cre newborn mice. The arrows mark the region where posterolateral processes protrude from the presphenoid body.

Lmo4 mutant mice exhibit homeotic-like transformations in their rib cage and cervical vertebrae. LMO proteins and their cofactor Ldb-1 have been implicated in regulating the transcriptional activity of various homeobox proteins, via either direct or indirect interactions with these proteins (2, 20). We explored whether Lmo4 mutant mice manifested any patterning defects reminiscent of those that occur in Hox-deficient mice. The skeletons of wild-type, Lmo4^+/-, and Lmo4^-/- mice were stained with alizarin red and Alcian blue, which are specific for bone and cartilage, respectively. Table 1 summarizes the homeotic-like transformations observed in Lmo4 mutants (E18.5 embryos and newborn mice). A variety of malformations and segment identity defects were observed in both Lmo4^-/- and Lmo4^+/- mice, but with variable penetrance. Lmo4^-/- mice (6 of 21) displayed defects in their rib cage, in which the eighth rib was aberrantly attached to the sternum (Fig. 4A versus B). Most of the attachment occurred on the right side, but two embryos were noted to have the eighth rib attached to the sternum on both sides, resulting in bilateral rib attachment (data not shown). One Lmo4 heterozygote also showed attachment of the eighth rib to the sternum. Asymmetric attachment, i.e., disorganized attachment of ribs to the sternum, was frequently observed in Lmo4 mutants (Fig. 4C versus D; Table 1): 7 out of 21 Lmo4^-/- and 4 out of 27 Lmo4^+/- mice. In severe cases, the first rib was not attached to the body of the sternum. In addition to defects within the rib cage, Lmo4 mutants displayed infrequent homeotic transformations of their cervical vertebrae. Furthermore, in 2 out of 21 embryos, the anterior tubercule was found attached to C7 instead of C6 (Fig. 4E versus F). Partial fusion of C2 and C3 vertebrae was observed in one Lmo4 mutant (Fig. 4G).

View larger version (75K): [in this window] [in a new window]   FIG. 4. Skeletal abnormalities in Lmo4 mutant mice. Bone and cartilage were stained with alizarin red and Alcian blue, respectively. (A to D) Ventral view of sternum and ribcage in wild-type mice (A and C) and Lmo4 mutants (B and D). (B) Aberrant attachment of the eighth rib to the sternum is indicated by a solid arrow. (D) Asymmetric alignment of the ribs is shown. (C) Symmetric alignment occurs in wild-type mice. In some Lmo4 mutants (F), anterior tubercules (AT) were attached to C7 (F) instead of C6, as occurs in wild-type mice (E). (F) One AT appears to be attached to both C6 and C7. (G) In another Lmo4 mutant, partial fusion of C2 and C3 was observed. AT stained with Alcian blue are indicated by white arrows. Vertebrae C2 to C7 are indicated by arrows.

View larger version (139K): [in this window] [in a new window]   FIG. 5. Cranial nerve malformation in Lmo4 mutant embryos. Wild-type (A), heterozygous (B), and homozygous Lmo4 (C and D) embryos at E9.5 were stained for neurofilaments using antibody 2H2. Fusion of cranial nerves IX and X, which occurs in 50% of Lmo4-/- and 25% of Lmo4+/- embryos, is indicated by arrows. In 2 out of 10 Lmo4-/- embryos, abnormal staining of axons surrounding ganglion V was observed (arrowhead in panel D). Cranial nerves V, VII, IX, and X and branchial arch II (BAII) are indicated.

View larger version (35K): [in this window] [in a new window]   FIG. 6. Targeted disruption of the mouse Deaf-1 gene. (A) Partial restriction map of the mouse Deaf-1 gene (top) and structure of the Deaf-1 targeting vector (bottom), which contains the thymidine kinase and neomycin resistance (neor) genes, both under the control of the mouse PGK promoter. Homologous recombination results in disruption of two exons encoding the SAND domain, represented as open boxes. The flanking probe used for Southern blot analysis is shown as a black bar. Abbreviations for restriction sites: Bg, BglII; B, BamHI; K, Asp718; R1, EcoRI; X, XhoI. (B) Generation of Deaf-1+/- mice. Deaf-1 chimeras were crossed with Gata1-cre transgenic mice to generate F1 progeny heterozygous for the targeted Deaf-1 allele. DNA was digested with EcoRI, and Southern blot analysis was performed using the 1.2-kb BglII-EcoRV probe indicated in panel A. (C) Protein lysates (30 µg) derived from E16.5 embryos were analyzed by Western blotting using a polyclonal antibody raised against Deaf-1. No Deaf-1 protein was observed in Deaf-1-/- embryos. The faint band at 46 kDa appears in both wild-type and -/- embryonic extracts and represents a nonspecific cross-reactive product. 293T cells transfected with an expression vector encoding hemagglutinin-tagged Deaf-1 served as a control. Western blot analysis using antitubulin antibody (-tubulin) confirmed equal loading.

View larger version (72K): [in this window] [in a new window]   FIG. 7. Defective neural tube closure and skeletal abnormalities in Deaf-1-deficient embryos. Deaf-1 mutant embryos (B) fail to close their neural tube by E10.5 and show outgrowth of the mid-hindbrain region compared to wild-type embryos (A). The penetrance of this phenotype on a mixed genetic background was 80%. (C to G) Bone and cartilage of wild-type (C) and Deaf-1 mutants (D to G) were stained with alizarin red and Alcian blue to analyze potential skeletal defects. Five out of twelve Deaf-1 mutants showed attachment of the eighth rib to the sternum (D) as seen in Lmo4 mutants. Two out of 35 Deaf-1+/- (E and G) and 2 out of 12 Deaf-1-/- (F) embryos showed bifurcation or fusion of ribs. The eighth and ninth ribs were bifurcated (E), while the first and second ribs appeared to be fused, as indicated by the arrows (F and G). Only one Deaf-1-/- embryo (F) showed fusion of the first and second ribs, and the anterior tubercule (AT) was attached to C7 instead of C6, as indicated by the arrow. In a Deaf-1 heterozygote (G), the first rib was attached to C7 instead of T1 and the AT was attached to C5 instead of C6.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

 
Members of the Lmo family of transcriptional coregulators have distinct expression profiles. Lmo4, the fourth member of this class, is widely expressed throughout embryonic development and in adult tissues. In contrast, Lmo1, Lmo2, and Lmo3 exhibit patterns of expression that are more tissue restricted. Within the embryo, Lmo4 is predominantly expressed in the CNS and peripheral nervous system, the thymus, the epidermis, and other epithelial tissues (21, 33). In the adult, high mRNA levels are found in brain, thymus, heart, mammary gland, skin, lung, liver, and spleen (21, 31, 33, 41). We report here that targeted disruption of the murine Lmo4 gene leads to early neonatal lethality. Multiple defects were evident in these mice, including failure of the neural tube to close, sphenoid bone abnormalities, and aberrant skeletal patterning. No anomalies were apparent in the major organs or within the hemopoietic compartment. Despite prominent expression of Lmo4 in lymphocytes, no defects were found in the ability of Lmo4^-/- ES cells to reconstitute the lymphoid compartment of Rag2-deficient mice (unpublished data).

Strikingly, exencephaly and homeotic transformations were also observed in mice lacking Deaf-1, a transcription factor that has been demonstrated to interact directly with Lmo4. Deaf-1, like Lmo4, displays a wide tissue distribution in adult tissues, including brain, lung, and skin (17, 23). This factor comprises a SAND domain with a conserved KDWK core that mediates DNA binding and a MYND domain consisting of a potential zinc-binding motif involved in protein-protein interactions. In Drosophila, Deaf-1 has been established to be essential for early embryonic development (39). Arrest usually occurred prior to zygotic gene expression, but some embryos developed into larvae that exhibited segmentation defects with variable severity. These defects included loss of segments and abnormal segment development along the anterior-posterior axis.

Lmo4 and Deaf-1 mutant mice exhibit common as well as distinct phenotypes. Both genes appear to be required for closure of the neural tube. Fifty percent of Lmo4 mutants exhibited exencephaly, while up to 80% of Deaf-1-deficient mice showed this defect. Unlike Deaf-1 mice, Lmo4 mutants without exencephaly died within a few minutes of birth. In the developing mouse, high levels of Lmo4 mRNA are present within neural crest cells, in addition to motor neurons, sensory neurons, somites, and Schwann cell progenitors (6, 21, 33). Specific targeting of the Lmo4 gene in either neural crest lineage cells or neuronal cells within the CNS, using Wnt1-cre or Nestin-cre transgenic mice, respectively, led to perinatal lethality or growth retardation (data not shown). Thus, while it is not clear what the underlying causes of neonatal lethality are, it is apparent that Lmo4 expression is required in these cell derivatives for proper development. Lmo4 but not Deaf-1 mutant mice showed abnormalities in the sphenoid bone at the base of the skull with complete penetrance. The lateral processes emanating from the presphenoid body were absent in Lmo4 homozygous mice, and in exencephalic Lmo4 mutants both the presphenoid body and trabecula basal plate were apparently missing. The biological consequences of these defects are not well understood.

Lmo4 and Deaf-1 mutant mice exhibit defects in their cervical vertebrae and rib cage. In addition, some of the transformations evident in Lmo4 and Deaf-1 mutants affected both homozygotes and heterozygotes, suggesting that gene dosage is important for correct function. In both Lmo4 and Deaf-1 mutants, the eighth rib was found aberrantly attached to the sternum. Asymmetric attachment of ribs was visualized frequently in Lmo4-null mice but rarely in Deaf-1 mutants. Conversely, bifurcated ribs were noted in Deaf-1 mutants but not in Lmo4-deficient mice. Transformations of cervical vertebrae were observed infrequently in both Lmo4 and Deaf-1 mutants. In contrast, cranial nerve defects in the hindbrain region were only evident in Lmo4 mutants. This probably arises from improper boundary formation within rhombomeres, caused by defective neuronal pathfinding. It is possible that Lmo4 plays a role in axon pathfinding in the hindbrain, similar to that of Hoxa2 (10).

The transformations observed in Lmo4 and Deaf-1 mutants and the cranial nerve defects evident in Lmo4 mutants are frequently seen in Hox-deficient mice, suggesting that Lmo4 and/or Deaf-1 alters the expression or activity of these homeobox proteins. Multiple genes within paralogous Hox groups 3 and 4 exhibit complex rib attachment and sternum phenotypes in knockout mice. Moreover, several Hox genes (such as Hoxa2, Hoxa3, and Hoxb3) expressed in the anterior boundary of the hindbrain lead to defects in cranial nerve patterning when disrupted (36). Hox transcription factor genes may represent targets of Lmo4 and Deaf-1. Alternatively, Lmo4/Deaf-1 may act as cofactors for Hox proteins and influence their activity. In the latter case, a shift in Hox gene expression would not be expected in either Lmo4 or Deaf-1 mutants, but rather, the ability of specific Hox factors to activate or repress their target genes would be altered. Deaf-1 was originally identified as a cofactor for the Hox protein Deformed, which is required for the development of structures derived from the mandibular and maxillary segments in Drosophila (27). It is tempting to speculate that Deaf-1 (and Lmo4) plays a parallel role as a Hox cofactor in mammals. Interestingly, Ldb1, a cofactor for Lmo4 and other LIM domain proteins, has been shown to bind and/or influence the function of Hox proteins, including Otx, Bicoid, and ftz, and the LIM homeodomain proteins Lhx1, Lhx3, and Lmx, (1, 3, 14, 19, 28). Removal of the mouse Ldb1 gene leads to early embryonic lethality associated with multiple patterning defects during gastrulation, including truncation of the anterior-to-hindbrain structures (29). Some of these phenotypes may be mediated via Hox or other homeodomain proteins. Taken together, it seems likely that the presumptive Lmo4/Deaf-1 or Lmo4/Deaf-1/Ldb1 complexes play a role in modulating Hox function in specific cell types.

Both Lmo4 and Deaf-1 mutants demonstrate homeotic-like transformations that vary in penetrance. Indeed, many of the homeotic transformations in single Hox mutants exhibit low penetrance and variable expression until compound targeted mice are generated. For example, targeted deletion of all HoxB genes results in a more penetrant and severe phenotype that represents the sum of those observed in single HoxB gene mutants (26). In addition, compound mutants for paralogous group genes (Hoxa-4, Hoxb-4, and Hoxd-4) show more complete homeotic transformations and a dose-dependent increase in the number of transformed vertebrae relative to that in single mutants (16).

There is increasing evidence that LMO-mediated interactions have functional relevance. Lmo2 has been established to form multiprotein transcriptional complexes in vivo with the hemopoietic transcription factors Scl/Tal-1 and Gata-1 and the ubiquitous regulators E47 and Ldb1 (42). Biochemical analyses of hematopoietic nuclear extracts has provided further evidence that these high-molecular-weight complexes exist (40). Mice deficient in Lmo2, Scl, and Gata-1 have established a close functional relationship between these proteins in the hematopoietic system. The skeletal transformations and defective neural tube closure reported here for the Lmo4 and Deaf-1 mutants suggest that Lmo4 and Deaf-1 form a physiological protein complex in specific cell types. The distinct phenotypes apparent in Lmo4 mutants further reveal that Lmo4 possesses Deaf-1-independent functions. Elucidation of the targets of Lmo4 and Deaf-1 should provide insight into the molecular mechanisms underlying the complex phenotypic abnormalities observed in Lmo4- and Deaf-1-null mice.

	ACKNOWLEDGMENTS

 
We thank R. Bronson for histologic examination of Lmo4^-/- sections, A. McMahon for Wnt1-cre transgenic mice, and J. Huggenvik for NUDR antibody. We are grateful to A. Voss for critical review of the manuscript.

J.V., G.L., and E.S. were supported by the Victorian Breast Cancer Research Consortium, Melbourne, Australia.

	FOOTNOTES

 
* Corresponding author. Mailing address: Division of Hematology-Oncology, Children's Hospital and Dana-Farber Cancer Institute, Department of Pediatrics, and Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115. Phone: (617) 632-3564. Fax: (617) 632-4367. E-mail: Stuart_Orkin{at}dfci.harvard.edu.

Present address: Biogen Idec, Exploratory Science, Cambridge, MA 02142.

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