Files in this Data Supplement:

• Supplemental file 1 - Supplemental text (materials and methods, results, and discussion) and Fig. S1 (Strategy to detect the RAG-induced nicks on the top or bottom strands), S2 (Detection of RAG cleavage sites on the top and bottom strands of the bcl-2 Mbr), S3 (Detection of RAG cleavage sites by denaturing PAGE on a 339-bp fragment containing bcl-2 Mbr), S4 (Effect of supercoiling on RAG complex nicking at the bcl-2 Mbr on a plasmid DNA), S5 (RAGs induce double-strand breaks on symmetric bubble and heterologous loop DNA structures), and S6 (Use of a prenicked bubble to confirm the identity of a RAG-nicked bubble structure).
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