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Dimer Formation in Dictyostelium discoideum
Ruchira Engel,2,
Mieke Blaauw,1
Antonie J. W. G. Visser,2 and
Peter J. M. van Haastert1*
Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands,1 MicroSpectroscopy Centre, Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands2
Received 31 January 2005/ Returned for modification 18 March 2005/ Accepted 25 May 2005
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ABSTRACT |
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subunits. However, Dictyostelium discoideum cells lacking the phosducin-like protein PhLP1 display defective rather than enhanced G protein signaling. Here we show that green fluorescent protein (GFP)-tagged Gß (GFP-Gß) and GFP-G subunits exhibit drastically reduced steady-state levels and are absent from the plasma membrane in phlp1– cells. Triton X-114 partitioning suggests that lipid attachment to GFP-G occurs in wild-type cells but not in phlp1– and gß– cells. Moreover, Gß dimers could not be detected in vitro in coimmunoprecipitation assays with phlp1– cell lysates. Accordingly, in vivo diffusion measurements using fluorescence correlation spectroscopy showed that while GFP-G proteins are present in a complex in wild-type cells, they are free in phlp1– and gß– cells. Collectively, our data strongly suggest the absence of Gß dimer formation in Dictyostelium cells lacking PhLP1. We propose that PhLP1 serves as a cochaperone assisting the assembly of Gß and G into a functional Gß complex. Thus, phosducin family proteins may fulfill hitherto unsuspected biosynthetic functions. |
INTRODUCTION |
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subunits (18, 32, 53, 57, 64, 69). In so doing, they are thought to function as a cellular "sink" which sequesters free Gß subunits following their dissociation from receptor-activated G proteins (2, 19, 33, 41, 58). As G protein-coupled receptors only couple to G
ß trimers, the sequestration of Gß attenuates transmembrane signaling. Thus, phosducin family proteins may adapt the cell's sensitivity to extracellular signals.
Being interested in factors underlying the adaptation of G protein-mediated chemotactic signaling in Dictyostelium discoideum, we recently identified three Dictyostelium Phd-like protein genes (3). To test whether PhLP1, the Dictyostelium protein that is most similar to mammalian Phd and PhLP, is involved in modulating G protein signaling, we analyzed phlp1 knockout cells. Surprisingly, G protein signaling is completely defective rather than enhanced in phlp1– cells, which exhibit a phenotype that is remarkably similar to that of gß knockout cells. Fluorescence confocal microscopy experiments with cells expressing green fluorescent protein (GFP)-tagged Gß (GFP-Gß) or GFP-G fusion proteins indicated that Gß complexes are absent from the plasma membrane of phlp1– cells, providing a possible explanation for the abrogation of signal transduction (3). These findings suggested that Gß and G fail to be assembled into a Gß complex in phlp1– cells or that the complex is not properly routed to the plasma membrane in these cells.
In this paper, we have further investigated the Gß defect in phlp1– cells. We show that steady-state levels of (GFP-tagged) Gß and G subunits are dramatically reduced and that these proteins are detected in the cytosol when the PhLP1 protein is not present. Triton X-114 partitioning experiments suggest that G is not lipid modified in phlp1 knockout cells. Prenylation is normally the first step in a sequence of posttranslational modification events following Gß dimerization (22, 70) and is essential for membrane association (45, 60). Moreover, a complementary combination of in vitro biochemical and in vivo spectroscopic experiments strongly suggests that Gß and G do not form a complex in the absence of PhLP1: tagged Gß and G subunits are not detectably coimmunoprecipitated, and cytosolic diffusion measurements using fluorescence correlation spectroscopy (FCS) reveal that the diffusion of GFP-G expressed in phlp1– cells is similar to that of free GFP-G monomers in cells lacking Gß.
Collectively, our data suggest that the phosducin-like protein PhLP1 serves a role in Gß dimer formation. We propose that PhLP1 may function as a cytosolic cochaperone for Gß assembly.
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MATERIALS AND METHODS |
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subunits. The gß– (LW6) and phlp1– knockout cell lines, as well as cells expressing either GFP-Gß or GFP-G, have been described previously (3, 36, 68).
Plasmid construction and transfection.
An expression plasmid encoding both GFP-tagged Gß and hemagglutinin (HA)-tagged G (HA-G) was constructed as follows. DNA encoding Dictyostelium G was isolated as a BglII-SpeI fragment from a pGEM-T G-Easy construct (3) and subcloned in pMB74 downstream of the actin15 promoter. The Dictyostelium expression vector pMB74 is derived from pMB12Neo (37) by replacement of the 2H3 terminator with an actin8 terminator. A double-stranded oligonucleotide containing a Dictyostelium translational initiation site (A5ATG) and a sequence encoding an N-terminal HA epitope (MISYPYDVPDYA) was inserted in frame with the G coding sequence by ligating it as a BamHI-BglII fragment into the BglII site of G/pMB74. The resulting expression cassette, with HA-G codons flanked by an actin15 promoter and an actin8 terminator, was isolated by XbaI digestion, Klenow treatment, and ClaI digestion and subcloned in the SmaI and ClaI sites of a pLB5Neo-based expression plasmid encoding GFP-Gß (3). The resultant plasmid, pGß Express, contains tandem expression cassettes for GFP-Gß and HA-G.
To express a GFP-PhLP1 fusion construct, DNA encoding PhLP1 was amplified by PCR using the forward primer 5'-TCTCAGATCTAAAGAATGGAACAAAACATTTTAAATAG-3' and the reverse primer 5'-GGACTAGTATCGTCATTATCATCATCGGAC-3', with the DNA encoding the open reading frame of phlp1 as the template (3). The DNA fragment was subcloned in pGEM-T Easy (Promega) and sequenced. Subsequently, the phlp1 insert was released and ligated into the BglII and SpeI sites of MB74GFP. The MB74GFP plasmid is similar to MB74 but contains the S65T GFP gene behind the SpeI site. The final fusion protein consists of the open reading frame of PhLP1, two serines, and the complete S65T GFP protein. The plasmids were introduced into Dictyostelium cells by electroporation following standard procedures, and transfectants were selected with G418.
Western blot analysis.
Cells were lysed either directly by boiling in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer or by filter lysis and detergent solubilization (see below). Equal amounts of lysates were loaded; lysates containing GFP-Gß or GFP-G were applied to standard 10 or 12% SDS-PAGE gels. Lysates containing the small HA-G protein were applied to 15% Tricine-SDS-PAGE gels (54). Following electrophoresis, proteins were electroblotted onto polyvinylidene difluoride membranes (Millipore). For confirming equal loading, blots were stained with Ponceau S. For Western blot analysis, the membranes were blocked for 2 h in 5% low-fat milk in Tris-buffered saline plus Tween 20 (TBST) (20 mM Tris-HCl [pH 7.4], 137 mM NaCl, 0.05% Tween 20). Subsequently, membranes were incubated overnight at 4°C with polyclonal anti-GFP antibody ab6556 (Abcam) diluted 1:5,000 in blocking buffer, washed several times with TBST, incubated for 1 h at room temperature with a peroxidase-coupled sheep anti-rabbit antibody (Roche), washed several times with TBST and once with TBS without Tween 20, and developed with an ECL kit (Roche).
Crude cell fractionation. Cells were harvested from confluent 9-cm dishes and washed twice with phosphate buffer (pH 6.5). Cell pellets were taken up in 150 to 300 µl ice-cold lysis buffer (50 mM Tris-HCl [pH 7.5], 5 mM EDTA, 5 mM EGTA, 150 mM NaCl, 1 mM dithiothreitol, containing Complete protease inhibitor cocktail [Roche]) and lysed through a 3-µm Nuclepore filter (Whatman). Lysates were centrifuged for 5 min in an Eppendorf centrifuge at 4°C, and a sample of the supernatant was carefully removed while taking care not to take along any particulate matter. The pellets were washed and then resuspended in an original volume of lysis buffer. Equal samples of supernatant (cytosolic) and pellet (particulate) fractions were used for Western blot analysis.
Triton X-114 partitioning assay. Cells were prepared and lysed as described above. Subsequently, 100 µl lysate was diluted with 100 µl lysis buffer containing 2% Triton X-114, which had been precondensed thrice (4), and rotated for 40 min at 4°C. Insoluble material was removed by spinning for 15 min at 4°C in an Eppendorf centrifuge, and supernatants were subjected to phase partitioning by incubation at 30°C for 3 min and spinning for 5 min at room temperature. The upper, water phases were reextracted with an equal volume of 2% Triton X-114, and the lower, detergent phases were reextracted with 0.2% Triton X-114. Following partitioning at 30°C, detergent phases were combined, and the volume was adjusted with lysis buffer to match the volume of the water phase samples. Equal samples of water and detergent phases were used for Western blot analysis.
Immunoprecipitation. Cells were prepared and lysed as described above. All subsequent procedures were performed at 4°C. Following lysis, 100 µl lysate was diluted with 400 µl lysis buffer containing 1.25% Triton X-100 and rotated for 45 min. Insoluble material was removed by centrifugation for 15 min in an Eppendorf centrifuge, and the lysate was precleared twice by rotating with 50 µl 50% (vol/vol) protein A-Sepharose beads for 1 h and removal of the beads by centrifugation for 10 min. Precleared supernatants were then rotated for 1 h with 2 µl monoclonal anti-GFP antibody ab1218 (Abcam), and immunocomplexes were collected by rotation with 25 µl 50% (vol/vol) protein A-Sepharose beads for 1 h and centrifugation for 1 min. The beads were washed four times with lysis buffer containing 1% Triton X-100 and once with lysis buffer without Triton X-100 while transferring the beads to a fresh tube. Beads were taken up in 2x concentrated SDS-PAGE sample buffer, heated for 3 min at 95°C, and spun down for 5 min in an Eppendorf centrifuge. Supernatants were stored at –20°C prior to Western blot analysis.
Fluorescence correlation spectroscopy.
The diffusion measurements of GFP-tagged proteins in cells were performed on a ConforCor 2 (Carl Zeiss). The details of the setup have been described previously (23). In our experiments, GFP was excited with the 488-nm line from an argon-ion laser and focused into the sample with a water immersion C-Apochromat 40x lens objective (Zeiss). The excitation intensity was 
11 µW. The excitation and emission light was separated by a dichroic beam splitter (HFT 488/633). The fluorescence was detected by an avalanche photodiode after being filtered through a band-pass filter of 505 to 550 nm. The pinhole was set at 70 µm.
Cells from a confluent dish were transferred to a 96-chamber glass-bottomed microplate (Whatman, Inc.) and washed twice with potassium phosphate buffer (17 mM, pH 6.5). Measurements were performed for cells incubated in buffer at room temperature. Around 100 autocorrelation traces were obtained for each cell line from five measurements made at a randomly chosen spot in the cytoplasm of around 20 different cells. The expression levels in cells were too high for FCS measurements, and so the GFP was photobleached to acceptable fluorescence intensity levels by exposing the cells to a high-intensity laser beam (1 mW) for 1 s. The measurement duration for all cell lines was 10 s.
The data were analyzed using the software FCS Data Processor (61). The autocorrelation traces were fitted with a model describing Brownian motion of a single species in three dimensions (equation 1), with an additional offset term to account for artifacts caused by drifts in average fluorescence on time scales of >1 s arising from cellular and intracellular movement (5).
 | (1) |
In equation 1, G(
) is the autocorrelation function, N is the average number of molecules in the detection volume, Tdif is the average diffusion time of the molecules, and sp is the structural parameter. A global-analysis approach was used to fit up to five traces simultaneously with the Tdif linked and a fixed value of sp. The value of sp, determined from calibration measurements with a rhodamine 110 solution, was around 5. The translational diffusion coefficient D was calculated from the diffusion time Tdif by using equation 2,
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xy is the equatorial radius of the confocal volume element and is obtained from calibration measurements. |
RESULTS |
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complexes, PhLP1 was fused to GFP and expressed in phlp1– cells. This resulted in a cell line with normal development and a complete rescue of the aggregation-negative phenotype (unpublished observations). Confocal microscopy revealed that PhLP1-GFP is localized predominantly in the cytosol, without indications for any membrane enrichment (Fig. 1). Stimulation of these cells with 1 µM cyclic AMP in a perfusion chamber does not lead to a significant change in the fluorescence intensity of PhLP1-GFP in the cytosol (detection limit of 4% change in fluorescence intensity [50]) or any detectable translocation of PhLP1-GFP from the cytosol to other cellular compartments (unpublished observations). These observations suggest that PhLP may function in the cytoplasm and that we have no evidence for an interaction of PhLP1 with Gß complexes at the plasma membrane.
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are expressed at reduced levels and do not associate with the plasma membrane in phlp1– cells.
To be able to monitor the fate of Gß and G in intact cells, we expressed GFP-Gß or GFP-G fusion proteins in wild-type AX3 cells and in phlp1– cells (3). Complexes of Gß and G tagged with GFP variants have been demonstrated to be fully functional in Dictyostelium amoebae (26, 27). As a reference, we also employed gß– cells, which do not express Gß and therefore lack normal Gß complexes (36, 68).
Western blot analysis revealed that correctly sized proteins are expressed in all cell lines (Fig. 2A). However, the steady-state levels of GFP-Gß and GFP-G proteins are dramatically reduced (at least 20-fold) in phlp1– cells compared to the levels in wild-type cells. Such reduced protein levels were also observed for endogenously expressed Gß by use of an anti-Gß antibody (unpublished observations). Interestingly, the mRNA levels for Gß are not significantly different between control and phlp1– cells (Fig. 2B), suggesting that the reduced levels of Gß, GFP-Gß, and GFP-G proteins are due to increased instability of the proteins in phlp1– cells. The cellular level of GFP-G in gß– cells is also significantly lower than in control cells (Fig. 2A). This indicates that G subunits are less stable in the absence of Gß. Such a mutual dependency of binding partners for stable expression has been previously documented for various protein complexes, including Gß dimers (24, 51, 55, 60, 67).
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can be detected both in a cytosolic fraction and in a particulate fraction containing plasma membranes (Fig. 3). Jin and coworkers similarly reported a considerable amount of GFP-Gß (about 30%) to be present in cytosolic fractions of transfected Dictyostelium amoebae and claimed that the same holds true for endogenous Gß (27). In striking contrast to the situation for wild-type cells, GFP-Gß and GFP-G cannot be detected in particulate fractions of phlp1– transfectants, although they are detected in cytosolic fractions. Again, in gß– cells, GFP-Gß can combine with endogenous G and exhibits behavior similar to that in wild-type cells, whereas GFP-G does not reach the plasma membrane in default of a Gß partner. These results are fully in line with our previous fluorescence confocal microscopy studies (3) and explain why G protein signaling is defective in cells lacking the PhLP1 protein. Either a Gß complex is formed but not transported to its final destination, or the complex is not formed at all, with individual Gß and G subunits not being able to associate with the plasma membrane. In either case, this largely results in the demise of the nonfunctional protein.
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is not prenylated in phlp1– cells.
To further define the stage at which the formation of a functional Gß complex goes awry in phlp1– cells, we assessed whether G proteins are still posttranslationally modified. During normal Gß synthesis, a CAAX motif at the C terminus of immature G subunits is prenylated, followed by proteolytic removal of the last three residues (AAX) and carboxyl methylation of the now C-terminal prenyl-cysteine moiety (22, 70). The attachment of a prenyl group is mandatory for stable membrane association of Gß subunits (45, 60).
Lipid modification of G can be demonstrated by metabolic labeling with radioactive precursors but can also be investigated in a nonradioactive fashion by using the Triton X-114 partitioning assay originally devised for integral membrane proteins (4). In this assay, cell lysates containing Triton X-114 are prepared and then induced to undergo phase separation by transient warming to 30°C. Proteins carrying a significantly hydrophobic moiety, such as a prenyl group, are drawn from the water phase into the detergent phase. The partitioning assay has been used not only for small monomeric G proteins but also for heterotrimeric G proteins (28).
As can be seen in Fig. 4, substantial amounts of both GFP-Gß and GFP-G expressed in AX3 cells are drawn into the detergent phase after partitioning. This indicates that GFP-G is lipid modified in wild-type cells and that GFP-Gß must form a tight complex with endogenous G since it lacks hydrophobic modifications. When expressed in gß– cells, GFP-Gß also forms a complex with endogenous G proteins and therefore partitions into the detergent phase. However, GFP-G expressed in gß– cells can only be detected in the water phase. This indicates that G is not (efficiently) modified unless it is present in Gß heterodimers. Such a finding corroborates suggestions in the literature that the Gß complex is the substrate for the prenylation machinery (22).
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expressed in phlp1– cells could only be detected in the water phase after extraction and partitioning. Thus, G remains unmodified in phlp1– cells and fails to draw Gß into the detergent phase. A lack of lipid attachment in phlp1– cells might be explained by defective modification of an otherwise properly formed Gß complex or by the failure of Gß and G to form a complex in the first place.
Coimmunoprecipitation studies suggest that Gß and G do not form a complex in phlp1– cells.
To assess whether there is indeed a lack of Gß complex formation in phlp1– cells, we performed coimmunoprecipitation experiments. In order to be able to detect both Gß and G, we transfected AX3 and phlp1– cells with a plasmid which directs the coexpression of GFP-tagged Gß (GFP-Gß) and hemagglutinin epitope-tagged G (HA-G). Cell lysates were subjected to immunoprecipitation with an anti-GFP antibody, and subsequently aliquots of the precipitate were analyzed by Western blot experiments with antibodies recognizing GFP (for immunoprecipitated GFP-Gß) or HA (for coimmunoprecipitated HA-G).
As can be seen in Fig. 5, GFP-Gß can be immunoprecipitated from both wild-type AX3 and phlp1– knockout cell lysates. HA-G was clearly coprecipitated with GFP-Gß when these proteins were coexpressed in wild-type cells. In contrast, a similar band could not be detected in the precipitate of phlp1– cells coexpressing GFP-Gß and HA-G, even though significant amounts of GFP-Gß had been precipitated. Furthermore, we have mixed small amounts of a lysate prepared from wild-type cells expressing GFP-Gß and HA-G with large amounts of lysate from phlp1– cells in a 1:20 ratio and could easily observe the presence of the Gß dimer (unpublished observations). These results suggest that a normal Gß complex is not present at significant levels in phlp1– cells, and we propose that the levels of Gß and G in phlp1– cells are low because they are not in dimers and are hence destabilized, rather than the opposite, that dimers are rare because Gß and G levels are too low in the absence of PhLP1. Unfortunately we have not yet been able to induce Gß dimer formation in vitro by using a mixture of lysates from GFP-Gß/HA-G-expressing phlp1– cells (providing unassembled GFP-Gß and HA-G) with wild-type cells (providing phosducin).
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in phlp1– and gß– cells suggests absence of Gß complexes in phlp1– cells.
The coimmunoprecipitation experiments described above indicate that Gß and G subunits do not form a complex in cells lacking PhLP1. Further experiments were complicated by the reduced steady-state levels of GFP-Gß and GFP-G in phlp1– cells. To overcome this problem, and to substantiate our in vitro findings in an in vivo environment, we used FCS, which is a sensitive technique that can be used to monitor the diffusion of proteins expressed at low concentrations in living cells (20, 30, 43). The diffusion characteristics of a protein provide valuable clues about its aggregation state or intracellular interactions. Thus, the cytoplasmic diffusion coefficients obtained from FCS measurements can be used to discriminate free subunits from Gß complexes in different cell lines.
The 7-kDa G is much smaller than the 40-kDa Gß and, thus, diffusion characteristics of GFP-G would be more strongly affected than those of GFP-Gß when Gß no longer forms a complex. Therefore, we chose to analyze the diffusion of GFP-G in the cytoplasm of wild-type and mutant cell lines (Fig. 6 and Table 1).
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in phlp1– cells is shifted to higher values than that in wild-type AX3 cells. The slow diffusion of GFP-G in wild-type cells indicates that it is predominantly present as a complex in wild-type cells but not in phlp1– cells. To determine if the diffusion of GFP-G in phlp1– cells was indeed the diffusion of free GFP-G monomers, we measured the diffusion of GFP-G in gß– cells. As can be seen in Fig. 6, the diffusion coefficient distribution for GFP-G expressed in gß– cells strongly overlaps with that obtained for phlp1– cells, clearly indicating that GFP-G in phlp1– cells is free. The average diffusion coefficient of GFP-G molecules in gß– cells is very similar to the value obtained for GFP-G in phlp1– cells (14 and 15 µm2/s, respectively). These values are slightly smaller than the value of 20 µm2/s obtained for freely diffusing GFP monomers in Dictyostelium cells (52). Since GFP-G and GFP are similar in size (35 and 27 kDa, respectively), their diffusion coefficients are also expected to be similar. However, GFP is approximately globular (49) whereas G in a Gß dimer has a rod-like structure (63), and though it is not known how the structure changes when G is not associated with Gß, it is likely that it is (partially) unfolded. The difference in the shapes of GFP and GFP-G can thus be the reason for the discrepancy seen between their diffusion coefficients. Taken together, the FCS data strongly suggest that, in cells that are devoid of PhLP1, GFP-G molecules are present as free monomers. We therefore conclude that Gß complexes, if present at all, form a very small minority in living phlp1– cells. |
DISCUSSION |
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dimers in cells lacking phosducin-like protein PhLP1.
Dictyostelium amoebae lacking the phosducin-like protein PhLP1 exhibit a phenotype which is strikingly similar to the phenotype observed upon gß gene disruption (3). Our present findings suggest that the gß–-like phenotype of phlp1– cells is due to defective Gß dimer formation. We observed a dramatic reduction in Gß and G steady-state protein levels in phlp1 knockout cells, while mRNA levels were unaltered. This might be explained with the notion that Gß complex formation is required for stable expression of either partner (24, 51, 55, 60, 67). Our inability to detect posttranslational lipid attachment to GFP-G in phlp1 knockout cells would also be in keeping with a lack of Gß dimer formation. It has been suggested that Gß assembly precedes cytosolic prenylation of the C-terminal CAAX motif of G (22). Indeed, when we overexpress GFP-G in gß– cells which cannot form Gß complexes, the protein is unstable and does not seem to be lipid modified in Triton X-114 partitioning assays.
The absence of Gß dimer formation is also strongly suggested by our inability to coimmunoprecipitate GFP-tagged Gß and HA-tagged G in vitro after coexpression in phlp1– cells. Furthermore, in vivo FCS experiments revealed that the diffusion of GFP-G in phlp1– cells is similar to that in gß– cells, whereas it is significantly slower in wild-type AX3 cells. The average diffusion coefficient values can be explained assuming the presence of free GFP-G monomers in phlp1– and gß– cells and of GFP-Gß complexes in AX3 cells. Therefore, the FCS results indicate that Gß and G are indeed apart in cells which lack PhLP1.
Taken together, the above data strongly suggest that without the PhLP1 protein, Gß dimer formation is defunct in Dictyostelium cells. We emphasize that it cannot be ruled out that small amounts of Gß dimers are still being formed in phlp1– cells. Maybe such traces of Gß can still provide some modulation of (a subset of) effectors, but in the assays which we reported previously, we failed to detect any Gß function (3).
PhLP1: a cochaperone for Gß dimer formation?
Whereas the G subunit is a relatively small and flexible peptide lacking tertiary structure (7, 55), the Gß subunit exhibits complex folding. Gß is a member of the WD repeat protein family (47, 62), which in turn forms part of a larger family of ß-propeller proteins (46, 48). Gß proteins contain seven WD repeats which form seven ß sheets making up the blades of a propeller-like structure. The last blade is made up of one ß strand donated by the first WD repeat and three ß strands donated by the last, C-terminal repeat, forming a noncovalent "Velcro snap" (6) which closes the ring structure of the propeller. Such a repetitive and highly integrated structure may not allow proper Gß folding and/or assembly to occur spontaneously.
The structural difference between Gß and G is reflected in a difference of ease of production. G can be produced in any expression system, but Gß is more demanding. Assembly-competent Gß cannot be produced in Escherichia coli or wheat germ extracts (21, 44) and only inefficiently in rabbit reticulocyte lysates (11, 12, 44, 55). This indicates the need for an accessory factor during Gß synthesis which may have diverged between plant and mammalian cells and is in limiting supply in reticulocyte lysates. The need for one or more cellular factors is also indicated by the finding that assembly-competent Gß can only be collected from recombinant baculovirus-infected Sf9 cells during early infection stages, when host cell protein expression is not shut down yet (14, 21). Thus, Gß proteins may require molecular chaperones (10, 17) for their incorporation into functional Gß complexes.
A likely candidate as one of these factors is the group II chaperonin CCT (chaperonin containing TCP-1), which is also known as TRiC (34, 66). The barrel-shaped cylinder of CCT, a folding cage made up of two rings of eight subunits each, may provide a favorable environment for, and participate in, the folding and assembly of various proteins. Intriguingly, an unexpected proportion of yeast proteins interacting with CCT subunits harbor seven WD repeats (66). Indeed, this set of proteins includes STE4, the yeast Gß subunit. These findings have recently been verified for both STE4 and five other, unrelated WD repeat proteins in yeast (59).
Apart from protein folding, CCT has also been implicated in the assembly of multimeric proteins. The WD repeat domain of the VHL tumor suppressor protein has been shown to bind to CCT prior to VHL assembly with the elongin BC complex (8, 16). Moreover, with the help of five additional cofactors, - and ß-tubulin are dimerized following their folding by CCT (15, 66). Thus, CCT, aided by cochaperones, may assist both folding and assembly of multimeric proteins, including WD repeat proteins such as Gß.
The picture that emerges from our studies on Dictyostelium phlp1– cells is that Gß complex formation is abolished in the absence of PhLP1. It is unlikely that PhLP1 deficiency leads to a loss of function of the CCT machinery, as the CCT machinery is absolutely required for actin and tubulin folding and its absence is lethal in yeast (35, 65). Rather, it is tempting to speculate that PhLP1 serves a role as a cofactor cooperating with CCT in the formation of native Gß heterodimers. Interestingly, mammalian PhLP, but not Phd, has been shown to coimmunoprecipitate CCT subunits (42).
While the current work was under review, three reports were published providing substantial support for the hypothesis that PhLP is a molecular chaperone for Gß assembly (25, 39, 40). The structure of the complex between CCT and PhLP reveals PhLP binding at the apical top of CCT, above the folding activity. This leads to the hypothesis that PhLP delivers its binding partner (possibly Gß) to the CCT complex for folding (40). Consistent with this hypothesis is the observation that RNA interference inhibition of the CCT subunit TCP-1 leads to a strong reduction of the level of Gß subunits (25). Very recently, Lukov et al. (39) have shown that reduced expression of PhLP by RNA interference results in inhibition of Gß expression and G protein signaling, similar to PhLP-null cells in Dictyostelium (3) and Cryphonectria parasitica (29). Furthermore, Lukov et al. demonstrated that the inhibition of G protein signaling is due to an inability of nascent Gß to form dimers, as also demonstrated in the present work. They provide a model in which PhLP binds to Gß and stabilizes the nascent Gß polypeptide until G will associate and PhLP will dissociate to catalyze another round of assembly (39). Phosphorylation of PhLP at Ser18 to Ser20 enhances binding to CCT and is essential for Gß folding (39). However, the role of CCT remains unclear, since a PhLP mutant with reduced CCT binding (PhLP133-135A) is fully capable in Gß folding (39).
The structure of Phd-Gß complexes may provide clues as to what the cochaperoning role of phosducin-type proteins could possibly be. Phd interacts with blades 6 and 7 of native Gß and can induce conformational changes in that region while transporting Gß away from the membrane (13, 38). Intriguingly, during Gß biosynthesis, blade 7 must be formed by bringing together remote ß strands from the N-terminal and C-terminal WD repeats of Gß (6). Also, assembly-competent Gß has an open structure with a Stokes radius larger than that of native Gß complexes (21, 55, 56). Thus, one might hypothesize that molecules such as PhLP facilitate ring closure of the Gß propeller while Gß and G assemble. The fact that phlp1– cells grow significantly more slowly than both wild-type and gß– cells (3) could indicate that the cellular chaperone machinery is to some extent obstructed in the absence of PhLP1 or that PhLP1 may assist in the folding of other proteins that are required for optimal cell growth.
Three families of PhLP proteins have been recognized, with one member of each family in Dictyostelium (3). The PhLP1 subfamily incorporates the mammalian PhLP involved in Gß folding (39) and the PhLP proteins from Cryphonectria parasitica and Dictyostelium that are essential for Gß functioning and folding (3, 29). Members of the PhLP2 and PhLP3 families bind Gß poorly and have no Gß-malfunctioning phenotypes. Deletion of PhLP2 is lethal in both Dictyostelium and yeast (3, 9). Interestingly, in yeast, PhLP2 forms a complex with CCT and VID27, a protein with a WD40 propeller structure similar to that of Gß (1). Deletion of PhLP3 in yeast and Dictyostelium is not lethal (3, 9), but genetic evidence suggests that the yeast PhLP3 family member (named Plp1p) transfers the nascent ß-tubulin polypeptides to the CCT folding apparatus (31). An interesting possibility that the PhLP families act as chaperones for the assembly of several proteins arises.
In conclusion, we have found that cells lacking the phosducin-like protein PhLP1 are not able to form functional Gß dimers, and we propose that this defect is related to an essential cochaperone function of PhLP1 during Gß assembly.
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ACKNOWLEDGMENTS |
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The work by R.E. was financially supported by the Research Council for Earth and Life Sciences of the Netherlands Organization for Scientific Research.
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FOOTNOTES |
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These authors contributed equally to this work.
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REFERENCES |
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