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Molecular and Cellular Biology, January 2005, p. 751-766, Vol. 25, No. 2
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.2.751-766.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

DNA Methyltransferase 3b Regulates Nerve Growth Factor-Induced Differentiation of PC12 Cells by Recruiting Histone Deacetylase 2

Shoumei Bai,1 Kalpana Ghoshal,1 Jharna Datta,1 Sarmila Majumder,1 Sung Ok Yoon,1,2 and Samson T. Jacob1*

Department of Molecular and Cellular Biochemistry, College of Medicine,1 Center for Molecular Neurobiology, The Ohio State University, Columbus, Ohio2

Received 13 September 2004/ Accepted 14 October 2004


   ABSTRACT
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
To elucidate the role of epigenetic reprogramming in cell- or tissue-specific differentiation, we explored the role of DNA methyltransferases (Dnmts) in the nerve growth factor (NGF)-induced differentiation of PC12 (pheochromocytoma) cells into neuronal cells. The mRNA and protein levels of de novo methyltransferase Dnmt3b increased, whereas those of Dnmt3a and Dnmt1 decreased, during NGF-induced neurite outgrowth. Dnmt3b localized in the nucleus, as well as in the growing neurites. When the expression of Dnmt3b was inhibited by antisense or small interfering RNA, PC12 cells continued to proliferate and failed to generate neurites. Cells depleted of Dnmt3b were unable to exit the cell cycle even after 6 days of NGF treatment. Furthermore, this failure in differentiation correlated with significant attenuation in tyrosine phosphorylation of TrkA (a marker for NGF-induced differentiation) and reduced the expression of neuronal markers, Hu antigen, and MAP2. The methyl-CpG content of the PC12 genome or the methylation status of repetitive elements was not significantly altered after differentiation and was not affected by Dnmt3b depletion. This was consistent with the ability of the catalytic-site mutant of Dnmt3b to induce differentiation in Dnmt3b-depleted cells after NGF treatment. The Dnmt3b-mediated differentiation was attributed to its N-terminal domain, which recruits histone deacetylase 2 (Hdac2), as demonstrated by (i) impeding of differentiation by the Hdac inhibitors, (ii) facilitation of the differentiation process by overexpression of the N-terminal domain of Dnmt3b, (iii) higher Hdac activity associated with Dnmt3b after NGF treatment, and (iv) coimmunoprecipitation and cosedimentation of Dnmt3b specifically with Hdac2 in a glycerol density gradient. These data indicate a novel role of Dnmt3b in neuronal differentiation.


   INTRODUCTION
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
Epigenetic control of gene expression mediated by cytosine methylation in DNA, histone modifications, and chromatin remodeling plays a critical role in proliferation, cell cycle withdrawal, and terminal differentiation. These epigenetic modifications can cause specific changes in brain functions (29, 54, 55). For instance, mutation in the brahma-related (Brg1) gene, the essential subunit of the SWI/SNF chromatin remodeling complex, causes a retinal differentiation defect in zebrafish (26). Histone deacetylation is essential for oligodendrocyte differentiation of the neonatal cortical progenitor. Blocking of histone deacetylase activity by trichostatin A (TSA) inhibits the progression of progenitors to mature oligodendrocytes (37).

Our laboratory has been studying the roles of different factors involved in the epigenetic process, particularly DNA methyltransferases (Dnmts), histone deacetylases (Hdacs), and methyl-CpG binding proteins (MBDs), on gene expression (23, 24, 35). Methylation of DNA at position 5 of cytosine within CpG dinucleotides is the most abundant covalent modification in the eukaryotic genome (27, 31). Although CpG is usually underrepresented in much of the genome, short (500- to 2,000-bp-long) CpG regions, designated CpG islands, are found in the proximal promoter regions of almost 50% of mammalian genes. Methylation of CpG islands influences the local chromatin structure as a result of the binding of a class of proteins designated MBDs. These proteins in turn recruit corepressors that include Hdac and various chromatin-remodeling factors (9, 10, 18, 22, 43, 49). The formation of a large repressor complex in the promoter region prevents binding of the key transcription factors to the cognate elements, which leads to transcriptional silencing. Initiation of the process leading to gene silencing has made Dnmts the driving force behind this intricate epigenetic event.

DNA methyltranferases that catalyze the transfer of methyl groups from S-adenosyl-L-methionine (Ado-Met) to position 5 of cytosines have been characterized in different eukaryotes, including mammals (8, 11, 41). Three functional Dnmts, namely, Dnmt1, Dnmt3a, and Dnmt3b, have been identified in mammalian cells. Dnmt1 preferentially acts on hemimethylated CpG substrates and is involved in the maintenance of specific DNA methylation patterns during DNA replication (7). On the other hand, Dnmt3a and Dnmt3b exhibit de novo methyltransferase activities (40). In addition to the C-terminal catalytic domain, all of the Dnmts harbor a transcriptional repressor domain in their N-terminal regions. Targeted disruption of the Dnmt1 or Dnmt3b gene causes embryonic lethality in mice, indicating that they are essential for normal embryo development, whereas Dnmt3a null mice die shortly after birth (34, 40). Appropriate regulation of development by these Dnmts may be achieved through the interplay of DNA methyltransferases with chromatin-remodeling factors, histone-modifying enzymes, and transcription factors (2, 20, 43, 45).

DNA methyltransferases and their isoforms are expressed in a tissue- or cell-type-specific manner (7, 12, 38), which suggests their participation in distinct developmental or differentiation process. In order to elucidate the roles of different Dnmts in proliferation and terminal differentiation, we chose pheochromocytoma (PC12) cells that can be either maintained in the proliferating stage or induced to differentiate by nerve growth factor (NGF). PC12 cells have been widely used in studying different aspects of growth factor-mediated differentiation (48, 51). Here, we report selective up-regulation of Dnmt3b during NGF-induced neuronal differentiation. In contrast, the expression of both Dnmt1 and Dnmt3a decreases dramatically. When the Dnmt3b level is reduced by RNA interference, NGF-dependent differentiation is inhibited, suggesting an essential role of Dnmt3b in growth factor-mediated differentiation. Surprisingly, the role of Dnmt3b in the differentiation process is independent of its catalytic activity.


   MATERIALS AND METHODS
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Cell culture. PC12 cells were obtained from the American Type Culture Collection (CRL-1721) and maintained in collagen (Sigma)-coated culture dishes in RPMI 1640 medium supplemented with 10% horse serum and 5% fetal bovine serum (25). For NGF treatment, the cells were washed with serum-free medium and maintained in RPMI 1640 medium supplemented with horse serum (1%) and 50 ng of NGF (Promega)/ml.

Plasmid construction. All of the mutant expression vectors were generated by PCR using Vent polymerase (New England Biolabs) with pcDnmt3b as a template. pcDnmt3b was constructed by excision of the full-length mouse Dnmt3b cDNA from pBSK-Dnmt3b with EcoRV and BamHI and inserted into the same site of pcDNA3.1(–). Dnmt3b cDNA with the ATRX domain deleted ({Delta}ATRXDnmt3b; amino acids 423 to 522 deleted) was generated by two-round PCR amplification, following the PCR mutagenesis protocol described earlier (36) with some modifications. In the first round, two separate PCRs were performed. (i) The region upstream of the ATRX domain was amplified using forward vector-specific T7 primer (5'-TAATACGACTCACTATAGGG) and the reverse Dnmt3b-specific primer B1 (5'-ATCTTTCCTGCGTCGGGACAAACAGCGGTCTTCCAG). (ii) The region downstream of the ATRX domain was amplified using the forward Dnmt3b-specific primer F1 (5'-GACCGCTGTTTGTCCCGACGCAGGAAAGATTGGAAC) and the reverse primer B2 (5'-CGGGATCCTTCACAGGCAAAGTAGTCCTT). (Overlapping sequ-ences in the Dnmt3b-specific primers flanking the ATRX domain are und-erlined.) The two PCR products from the first round of PCR were purified, mixed in 1:1 molar ratio, and used for the second round of PCR, using the T7 and B2 primers to generate {Delta}ATRXDnmt3b. The PCR product was digested with EcoRV and BamHI and inserted into the same site of p3XFLAG-CMV (Sigma). An N-terminal deletion ({Delta}NDnmt3b; amino acids 1 to 528 deleted) was gener-ated by amplification using the forward primer 5'-GGGGTACCACCATGCGCCTGCAAGACTTCTTCTTCAC and the reverse primer B2 (see above). This product was digested with KpnI and BamHI and cloned into the same site of p3XFLAG-CMV. A catalytic domain deletion ({Delta}CDnmt3b; amino acids 590 to 777 deleted) mutant was generated by PCR amplification using the forward T7 primer and the reverse 5'-CGGGATCCGGACTCTGCACAGACTTCGGA primer, digested with EcoRV and BamHI, and cloned into the same sites of p3XFLAG-CMV (Sigma). The authenticity of the deletion mutants, as well as any aberrant PCR error, was determined by sequencing.

Generation of stable cell lines. To generate cell lines expressing antisense Dnmt3b, mouse Dnmt3b cDNA was excised from pBSK-Dnmt3b with EcoRV and BamHI and inserted into the same site of pcDNA 3.1(+) so that the Dnmt3b cDNA was in reverse orientation with respect to the cytomegalovirus (CMV) promoter. PC12 cells were transfected with this antisense Dnmt3b vector using the calcium phosphate precipitation method (4). The PC12 cells were transfected with {Delta}CDnmt3b vector mediated by Lipofectamine reagent (Invitrogen) to generate cells expressing the N-terminal domain of Dnmt3b. The transfected cells were cultured in medium containing G418 (500 µg/ml), and individual G418-resistant colonies were obtained by the serial-dilution method. To confirm the down-regulation of Dnmt3b, whole-cell extracts of these clones were subjected to Western blot analysis with Dnmt3b-specific antibody raised in our laboratory (35). Clones with significantly reduced Dnmt3b levels were further confirmed by immunofluorescence assay as described below. Two clones (numbers 28 and 21) expressing significantly reduced levels of Dnmt3b were used for further studies.

Transient transfection. PC12 cells (5 x 105) were seeded in six-well plates. Lipofectamine reagent was used for transfection of the wild-type Dnmt3b or Dnmt3b deletion mutants ({Delta}N, {Delta}C, or {Delta}ATRX), along with Dnmt3b small interfering RNA (siRNA) (3bsi; 40 nM). NGF was added 12 h posttransfection. For longer treatment (up to 6 days), The successive transfections were performed every other day. The morphological changes were monitored by photography using a digital camera (Nikon COOLPIX 4300) under a phase-contrast microscope (Nikon ECLIPSE TS100).

RNA interference assay. 3bsi was designed to target specifically the 3' untranslated region (UTR) of rat Dnmt3b cDNA. The specificity was confirmed by a BLAST search using the National Center for Biotechnology Information database, as well as a rat genomic database. No significant homology to the 21-bp siRNA sequence was found in the database. As a control, scrambled siRNA (Dharmacon) that did not exhibit homology to any coding region but with a similar GC content was used. To generate double-stranded siRNA, sense (5'-AGAUGACAGGUGCCCAGAGUU) and antisense (5'-CUCUGGGCACCUGUCAUGUUU) strands of siRNA (20 µM) were annealed in 1x siRNA annealing buffer [6 mM HEPES (pH 7.4), 20 mM KCl, and 0.4 mM Mg(OAC)2] for 1 min at 90°C, followed by 1 h of incubation at 37°C. For transfection, 5 x 105 PC12 cells were plated in a six-well plate 24 h earlier, and siRNA was added, along with 5 µl of Lipofectamine. For long-term treatment, cells were transfected with siRNA every 60 h.

Real-time quantitative reverse transcription (RT)-PCR analysis. Total RNA was isolated from PC12 cells using the guanidinium thiocyanate-acid phenol method (13) and treated with Turbo DNase 1 (Ambion), following the manufacturer's protocol. cDNA was synthesized from 3 µg of total RNA using random hexamers as primers and murine leukemia virus reverse transcriptase (Applied Biosystems), following the manufacturer's protocol. An aliquot of the cDNA (equivalent to 225 ng of RNA for Dnmt3a and -3b and Dnmt1 and 2.25 ng for 18S rRNA) was used for real-time PCR analysis. All real-time PCRs were carried out using the Mx3000 Multiplex Quantitative PCR System (Stratagene).

The following PCR primers were used for RT-PCR analysis: Dnmt1, 5'-GCTAAGGACGATGATGAGACGC and 5'-CTTTTTGGGTGACGGCAACTC; Dnmt3a, 5'-CAGCGTCACACAGAAGCATATCC and 5'-GGTCCTCACTTTGCTGAACTTGG; Dnmt3b, 5'-GAATTTGAGCAGCCCAGGTTG and 5'-TGAAGAAGAGCCTTCCTGTGCC; 18S rRNA, 5'-TCAAGAACGAAAGTCGGAGG and 5'-GGACATCTAAGGGCATCACA .

The optimum primer concentration was 150 nM for Dnmt3b, Dnmt1, and 18S rRNA and 100 nM for Dnmt3a. All PCR amplifications were performed using Brilliant SYBR Green QPCR Master Mix (Stratagene), with ROX as a reference dye in a 20-µl reaction volume. A standard curve for each cDNA was first generated using 10-fold serial dilutions (108 to 102 copies) of the respective cDNAs as templates. To create standard curves, rat 18S rRNA, Dnmt1, Dnmt3a, and Dnmt3b cDNAs were amplified by RT-PCR. The copy number of each cDNA expressed in PC12 cells was calculated from the standard curve and normalized to that of 18S rRNA. The PCR cycling conditions were as follows: initial denaturation at 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s, and a dissociation cycle of 95°C for 1 min and 55°C for 30 s (to check the formation of a primer dimer). The dissociation profiles of the amplified products indicated that none of the primer pairs generated dimers.

RT-PCR analysis. Total RNA from PC12 cells was subjected to RT-PCR for Dnmts with primers as described above. The number of PCR cycles for all primer pairs was 25, except Cox-1 (cytochrome C oxidase) (15 cycles). The PCR primer pairs used for Cox-1 were 5'-CCCCCTGCTATAACCCAATATCA and 5'-TCCCTCCATGTAGTGTGTGTAGCGAGTCAG.

Western blot analysis. Whole-cell extracts from PC12 cells were prepared by suspending the cell pellets in cell lysis buffer (50 mM Tris-HCl [pH 7.5], 10% glycerol, 2% sodium dodecyl sulfate [SDS], and 1 mM dithiothreitol). Total protein (50 to 200 µg) was resolved by SDS-polyacrylamide gel electrophoresis (PAGE), transferred to a nitrocellulose membrane, and subjected to immunoblot analysis. The following antibodies were used for this study: Dnmt1 (a generous gift from Shoji Tajima), Dnmt3a and -3b (raised in our laboratory) (35), Ku antigen (N3H10; Neomarker), Hdac1 (Cell Signaling and Upstate Biotechnology), Hdac3 (Cell Signaling), Hdac2 (Santa Cruz), and horseradish peroxidase-conjugated secondary antibodies (Amersham). The ECL Western blotting detection system (Amersham) was used except for Hdac1, which was detected using highly sensitive chemiluminescent horsesradish peroxidase substrate (Sigma). Competitive Western blot analysis was performed to confirm the specificity of Dnmt3b antibody. Extracts from NGF-treated PC12 cells were separated by SDS-PAGE in parallel lanes and transferred to nitrocellulose membranes. One half of the membrane was subjected to Western blot analysis with Dnmt3b antibody, and the other half was incubated with antibody containing a 50-fold excess (molar) of recombinant Dnmt3b (expressed in bacteria as histidine-tagged protein and purified with Ni-nitrilotriacetic acid resins) for 1 h before Western blot analysis (35).

Indirect immunofluorescence assay. PC12 cells were seeded at a density of 105/well in two-well chamber slides (Labtek). For neuronal differentiation, the cells were treated with 50 ng of NGF/ml for at least 6 days, fixed in 4% paraformaldehyde, and permeabilized with 0.3% Triton X-100. Nonspecific binding was eliminated by incubating the cells in blocking solution (1% bovine serum albumin, 10% fetal bovine serum, and 10% goat serum) for 1 h at room temperature. The cells were then washed with phosphate-buffered saline (PBS) and incubated with primary antibody overnight at 4°C. The cells were washed again with PBS and incubated with fluorescein isothiocyanate-conjugated anti-rabbit secondary antibody (for Dnmt3a or Dnmt3b) and tetramethyl rhodamine isothiocyanate (TRITC)-conjugated secondary antibody (anti-mouse for Hu antigen or MAP2) for an additional hour. The cells were mounted in the mounting medium supplemented with DAPI (4',6'-diamidino-2-phenylindole) to stain the nuclei and viewed using a fluorescence microscope (Nikon ECLIPSE 800).

Analysis of DNA replication. Equal numbers (105) of antisense Dnmt3b-transfected cells (numbers 21 and 28), the parental cells, and vector-transfected cells were cultured in 12-well plates for 6 days with or without NGF treatment. The cells were then washed with serum-free medium and incubated in the same medium for 1 h, followed by incubation with [3H1]thymidine (1 µCi/ml) (Amersham) for 4 h. The cells were then washed three times with PBS, and the DNA was precipitated with 10% trichloroacetic acid (TCA). The precipitated DNA was washed twice with 10% TCA and dissolved in 500 µl of lysis buffer (0.5 N NaOH, 0.5% SDS). An aliquot (400 µl) of the lysate was used for scintillation counting. Each sample was assayed in triplicate.

Analysis of BrdUrd incorporation. Parental PC12 cells, empty-vector-transfected cells, and Dnmt3b antisense clones (numbers 21 and 28) were either left untreated or treated with 50 ng of NGF/ml for 1 to 6 days. The cells were pulse-labeled with bromodeoxyuridine (BrdUrd) (10 µM; Sigma) for 4 h, fixed with 70% ethanol, denatured with 2 N HCl, and stained with anti-BrdUrd antibody (Sigma) for 2 h at 37°C, followed by staining with horseradish peroxidase-conjugated secondary antibody (Amersham). Color was developed with diaminobenzidine (Sigma), and the cells were counterstained with eosin Y (Sigma). The cells were photographed with a digital camera (Nikon COOLPIX 4300) under a phase-contrast microscope (Nikon ECLIPSE TS100).

Southern hybridization. Genomic DNA was isolated from untreated parental PC12 cells, 3bsi-transfected cells, and antisense Dnmt3b clones (numbers 21 and 28), as well as a vector-transfected control. DNA (5 µg) was digested with methylation-sensitive (HpaII) and -insensitive (MspI) enzymes, separated on a 0.8% agarose gel, and transferred to a nitrocellulose membrane, followed by Southern blot analysis with 32P-labeled, random-primed rat IAP (intracisternal A particle), rat AluI, and rat satellite 1 repeat elements. The rat IAP gene was generated by amplification with the primers 5'-GAATCCAACCGTGTTGCGTC and 5'-TGTTCTAACGACTTCCTCACATCG, specific for rat IAP repeats. The rat AluI repeat was isolated from rat genomic DNA digested with AluI, separated on an agarose gel, and cloned into the TA vector (Invitrogen). The rat satellite 1 probe was prepared as described previously (28).

TrkA phosphorylation. PC12 control cells and cells transfected with Dnmt3b siRNA (40 nM) were treated with NGF (50 ng/ml) for 10 min at 37°C. Whole-cell extracts were prepared in lysis buffer (20 mM Tris [pH 8.0], 137 mM NaCl, 0.5 mM EDTA, 10% glycerol, 1% NP-40, 10 mM sodium pyrophosphate, 10 mM NaF, 1 µg of aprotinin/ml, 10 µg of leupeptin/ml, 1 mM vanadate, 1 mM phenylmethylsulfonyl fluoride). Equal amounts of protein from cell lysates were subjected to an immunoprecipitation assay with pan-Trk-specific antibody (Santa Cruz), followed by Western blot analysis with the phosphotyrosine antibodies 4G10 (Upstate) and pY20 and pY99 (Santa Cruz). The blot was stripped off and reprobed with pan-Trk antibody to measure the total TrkA.

Immunoprecipitation analysis and histone deacetylase assay. Equal amounts of protein from the control and NGF-treated PC12 cells (parental or overexpressing the N-terminal domain of Dnmt3b [{Delta}Cdnmt3b]) in lysis buffer (see above) were subjected to immunoprecipitation with anti-Dnmt3b antibody or preimmune serum. The immune complex was pulled down with protein A agarose beads (Gibco), and the histone deacetylase activity was measured using a histone deacetylase assay kit (Upstate) following the manufacturer's protocol.

Coimmunoprecipitation analysis. PC 12 cells were treated with NGF for 6 days, and cell extracts were prepared in TNN buffer (50 mM Tris-HCl [pH 8.0], 150 mM NaCl, 0.5% NP-40, 1 µg of aprotinin/ml, 10 µg of leupeptin/ml, and 1 mM phenylmethylsulfonyl fluoride). The cell extract (1 mg of protein) was immunoprecipitated with Dnmt3b or Hdac2 (Santa Cruz) antibody. Dnmt3b preimmune serum or mouse immunoglobulin G (IgG) was used as a negative control. The immune complex was pulled down with protein A (for Dnmt3b) and protein G (for Hdac2), and the beads were washed three times with TNN buffer. The precipitated proteins were subjected to Western blot analysis with Dnmt3b and Hdac2 antibodies, followed by anti-Dnmt3b antibodies.

Glycerol density gradient centrifugation. Whole-cell extracts from control and differentiated PC12 cells were prepared in TNN buffer and loaded onto a 5 to 30% glycerol density gradient, followed by centrifugation at 39,000 rpm for 24 h (SW40Ti; Beckman). Eighteen fractions were collected from the bottom of the tube, and the proteins were separated by SDS-PAGE gel followed by Western blotting with Dnmt3a, Dnmt3b, and Hdac1 to -3 antibodies.


   RESULTS
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Dnmt1, Dnmt3a, and Dnmt3b are differentially expressed during NGF-induced differentiation of PC12 cells. As a first step to elucidate the role of DNA methyltransferases in NGF-induced differentiation of PC12 cells into neuronal cells, we analyzed the mRNA levels of Dnmt1, Dnmt3a, and Dnmt3b at different periods during NGF treatment by real-time RT-PCR. Treatment of PC12 cells with 50 ng of NGF/ml induced visible neurite outgrowth beginning at 24 h, and ~85% of the cells generated long neurites by the end of 6 days. During this time, Dnmt1 mRNA gradually decreased (Fig. 1A). Nearly fivefold reduction in Dnmt3a mRNA levels was observed after 24 h of NGF treatment (P < 0.01) (Fig. 1B), which was subsequently maintained at the reduced level. By contrast, the mRNA level of Dnmt3b increased progressively with time and was dramatically elevated after 6 days of NGF exposure (P < 0.01) (Fig. 1C). RNA samples without reverse transcription did not generate any PCR product (data not shown).



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  		FIG. 1. Dnmt1, -3a, and -3b are differentially regulated during PC12 cell differentiation. (A to C) Real-time RT-PCR analysis of Dnmt1, Dnmt3a, and Dnmt3b mRNAs during NGF treatment of PC12 cells. Total RNA isolated from untreated and NGF-treated (50 ng/ml) PC12 cells (1 to 6 days [d]) were treated with DNase1, converted to cDNA, and subjected to real-time quantitative PCR using primers specific for Dnmt1, Dnmt3a, and Dnmt3b cDNAs and 18S rRNA. The levels of Dnmt1, -3a, and -3b are presented as numbers of copies per 106 copies of 18S rRNA(t test; P < 0.01). The error bars indicate standard deviations. (D) Alteration in protein levels of DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b in response to NGF-induced PC12 cell differentiation. Whole-cell extracts from untreated PC12 cells and cells treated with NGF for different periods (1 to 6 days) were subjected to immunoblot analysis with antibodies against Dnmt1, Dnmt3a, Dnmt3b, and N3H10 (specific for Ku-70 autoantigen, used as a loading control). (E) Immunodepletion analysis of Dnmt3b. Extracts from NGF-treated PC12 cells were separated by SDS-PAGE in parallel lanes and transferred to nitrocellulose membranes. One half of the membrane was subjected to Western blot analysis with Dnmt3b antibody (–Ag), and the other half was preincubated with a 50-fold excess (molar) of exogenous recombinant Dnmt3b (expressed as His tag protein and purified with Ni-nitrilotriacetic acid resins) for 1 h before Western blot analysis was continued (+Ag). Ag, antigen. (F) Immunofluorescence analysis of Dnmt3a and -3b before and after differentiation of PC12 cells. PC12 cells were either left untreated (–NGF) or allowed to differentiate in the presence of NGF (50 ng/ml) for 6 days (+NGF). The cells were then stained for Dnmt3a and Dnmt3b using Dnmt3a- or Dnmt3b-specific antibody and DAPI (to locate the nucleus) and visualized under a fluorescence microscope. Bar, 100 µm.

 
We then determined whether the protein levels of DNA methyltransferases were affected by NGF treatment. Western blot analyses showed two- and eightfold decreases in Dnmt1 and Dnmt3a levels, respectively, after 1 day of NGF treatment (Fig. 1D). In contrast, the Dnmt3b protein level was relatively low in untreated PC12 cells and increased gradually for the duration of NGF treatment, which correlated with the mRNA level. The higher level of Dnmt3b in the differentiated PC12 cells is noteworthy, as it is very low in most of the adult tissues and cell types, as measured by Northern blotting and RT-PCR (42, 44). To demonstrate that the polypeptide detected by the antibody was indeed the Dnmt3b polypeptide, we preincubated the antibody with exogenous recombinant Dnmt3b prior to Western blot analysis. Significant reduction in the signal corresponding to the 100-kDa polypeptide upon immunodepletion confirmed its identity as Dnmt3b in PC12 cells (Fig. 1E, compare lanes +Ag and –Ag).

Next, we performed indirect immunofluorescence analysis to investigate the subcellular localization of Dnmt3a and Dnmt3b. The results correlated well with the Western blot data (Fig. 1F). Interestingly, Dnmt3b localized not only in the nucleus but also in the growing neuronal processes, while Dnnt3a was expressed exclusively in the nucleus. Together, these data indicate differential regulation of Dnmt3b expression during NGF-induced neurite outgrowth in PC12 cells.

Targeted down-regulation of Dnmt3b inhibits NGF-induced neurite outgrowth. The preferential increase in Dnmt3b expression following NGF treatment prompted us to investigate whether its up-regulation was critical for the differentiation process. This was achieved by transfecting PC12 cells with siRNA specific for the 3' UTR 3bsi to block its expression. Dnmt3b RNA depletion was obvious at 20 nM 3bsi after 48 h of transfection. The specificity of 3bsi-mediated inhibition was evident from the continued expression of Dnmt1 and Dnmt3a (Fig. 2A). The scrambled siRNA, which had no homology to any known cDNA, did not affect the expression of any of these genes (Fig. 2A). The majority (87%) of the cells transfected with the scrambled siRNA exhibited extensive neurite outgrowth after 6 days of NGF treatment (Fig. 2B and C), attaining a morphology similar to that of untransfected cells (Fig. 2F). In contrast, the cells transfected with 3bsi grew mostly in clusters, with a concomitant decrease in the population of neurite-bearing cells (12%) (Fig. 2C). This remarkable morphological difference between Dnmt3b-depleted cells and the parental PC12 cells was detectable as early as 3 days after NGF treatment (data not shown).



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  		FIG. 2. Targeteddown-regulation of Dnmt3b inhibits PC12 cell differentiation. (A) Selective down-regulation of Dnmt3b with 3bsi. Total RNA was isolated from PC12 cells transfected with scrambled siRNA (scramble) (40 nM) or 3bsi (20 to 60 nM) for 48 h, converted to cDNA (equivalent to 300 ng of RNA from each sample), and subjected to RT-PCR analysis with primers specific for Dnmt3b, Dnmt1, Dnmt3a, and Cox-1 cDNAs. (B and C) Inhibition of NGF-induced neurite outgrowth in PC12 cells treated with Dnmt3b siRNA. PC12 cells transfected with 3bsi RNA (40 nM) or scrambled siRNA (scramble) (40 nM) were treated with (+) 50 ng of NGF/ml for up to 6 days. The cells were viewed under a phase-contrast microscope, and images were captured with a digital camera. (B) Quantification of differentiating cells to assess the percentage of cells generating neurites at least two cell bodies in length. Approximately 200 cells were counted for each sample. The error bars represent the standard deviation of triplicate results. +, present; –, absent. (C) Microscopy image of cells transfected with either scrambled RNA or 3bsi. Bar, 250 µm. (D) Specific reduction of Dnmt3b level in stable cell lines expressing antisense Dnmt3b, as shown by immunoblot analysis. Total proteins isolated from two cell lines expressing antisense Dnmt3b (#21 and #28), untransfected PC12 cells (P), and pcDNA3.1 vector-transfected cells (V) were subjected to Western blot analysis using antibodies against Dnmt1, Dnmt3a, Dnmt3b, and Ku-70 autoantigen (N3H10). (E and F) Suppression of NGF-induced differentiation in stable cell lines expressing antisense Dnmt3b. Parental (P) and vector-transfected (V) cells and clones (#28 and #21) were allowed to differentiate in the presence of 50 ng of NGF/ml for 6 days. (E) Quantitative analysis of the percentage of cells bearing neurites. This figure represents the number of cells with neurites at least two cell bodies in length. The error bars represent the standard deviations of triplicate results. Approximately 200 cells were counted in each sample. (F) Representative microscopy image of control and Dnmt3b antisense cell lines. Bar, 50 µm.

 
To further confirm the role of Dnmt3b in the differentiation process, stable PC12 cell lines overexpressing antisense Dnmt3b RNA were generated. Reduced expression of Dnmt3b in stable cell lines was monitored by Western blot analysis (Fig. 2D) and indirect immunofluorescence assays (data not shown). Two clones (numbers 21 and 28) expressed significantly less Dnmt3b protein without the Dnmt3a and Dnmt1 levels being altered (Fig. 2D). For subsequent studies, we used these two lines and the vector (pcDNA3.1)-transfected cells. The number 21 and 28 cells responded poorly to NGF and grew in aggregate. Only a few cells at the peripheries of the clusters projected neurites (Fig. 2C). The morphological characteristics of these cells (Fig. 2F) were remarkably similar to those of cells treated with Dnmt3b siRNA (Fig. 2C). These results demonstrate that Dnmt3b expression is necessary for the induction of neuronal differentiation in response to NGF.

PC12 cells with reduced Dnmt3b continue to proliferate following NGF treatment. To test the possibility that the inefficiency of the Dnmt3b-depleted cells in generating neurites was due to their inability to exit the cell cycle, the DNA replication potentials of Dnmt3b-depleted number 21 and 28 lines were determined. Prior to NGF treatment, the replication potential of these cells was not significantly altered, as a level of [3H1]thymidine comparable to that in the control was incorporated in the cells. After NGF treatment for 6 days, however, the number 21 and 28 lines incorporated significant amounts of [3H1]thymidine (P < 0.05), while its uptake dramatically declined in the control cells (Fig. 3A). We also measured the proliferation potentials of these cells by BrdUrd incorporation assay, counting BrdUrd-positive cells after staining them with anti-BrdUrd antibody. At 24 h post-NGF treatment, there was a general decrease in the number of BrdUrd-positive cells among the Dnmt3b-depleted cells (Fig. 3). After 6 days, however, both cell lines continued to divide, as was evident from the extent of BrdUrd incorporation. The relatively greater reduction in BrdUrd uptake by the number 21 cell line correlated with its higher Dnmt3b level (compared to number 28 cells) (Fig. 2D). There was no significant BrdUrd uptake by the parental and vector-transfected cell lines after 6 days of NGF exposure (Fig. 3).



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  		FIG. 3. Cells depleted of Dnmt3b are unable to exit the cell cycle even after 6 days of NGF treatment. (A) DNA replication measured by thymidine incorporation. Equal numbers (105) of clones 21 and 28 and the parental cells (P) and vector control (V) were pulsed with [3H1]thymidine for 4 h. The cells were then washed three times with PBS and precipitated with 10% TCA. An aliquot (400 µl) of the lysate was counted in a scintillation counter. Each sample was assayed in duplicate, and the experiment was repeated three times (t test; P < 0.05). The error bars represent standard deviations. +, present; –, absent. (B) BrdUrd incorporation. Parental PC12 cells, vector-transfected cells, and number 21 and 28 cell lines were either left untreated or treated with 50 ng of NGF/ml for 1 to 6 days (d). The cells were pulse-labeled with BrdUrd (10 µM), fixed, and incubated with anti-BrdUrd antibody, followed by horseradish peroxidase-conjugated secondary antibody. The color was developed with diaminobenzidine, and the cell body was counterstained with eosin Y. Nuclei of the BrdUrd-positive cells were stained dark brown. Bar, 100 µm. (C) Quantitative analysis of BrdUrd incorporation (t test; P < 0.01). BrdUrd incorporation was represented as the percentage of BrdUrd-positive cells (dark-brown color) out of 1,000 cells counted from each sample.

 
Phosphorylation of TrkA, the NGF receptor, is reduced in Dnmt3b-deficient cells. The molecular mechanism of NGF-induced neuronal differentiation involves a signaling cascade initiated by binding of NGF to its receptor, TrkA, and the induction of TrkA autophosphorylation on tyrosine residues (48). To determine whether this signaling pathway was compromised in the Dnmt3b-depleted cells, we examined, by immunoprecipitation with pan-Trk antibody and Western blotting with anti-phosphotyrosine antibody, the tyrosine phosphorylation status of TrkA after NGF treatment in cells treated with Dnmt3b siRNA. The extent of tyrosine phosphorylation was reduced threefold in 3bsi-transfected cells compared to control cells, while the total TrkA protein levels did not change upon siRNA transfection (Fig. 4A). These data suggest that the failure to initiate the NGF signaling cascade might account for the impediment in neurite outgrowth observed in Dnmt3b-depleted cells.



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  		FIG. 4. Phosphorylation of TrkA is markedly reduced in cells with reduced Dnmt3b levels upon NGF treatment, with a concomitant decrease in the levels of the neuronal markers Hu antigen and MAP2. (A) Phosphorylation analysis. PC12 control cells and cells transfected with 3bsi (40 nM) were treated with (+) NGF (50 ng/ml) for 10 min. An equal amount of protein from the cell lysates was subjected to an immunoprecipitation assay with pan-Trk-specific antibody, followed by Western blot analysis with phosphotyrosine antibodies to determine the level of phosphorylated TrkA (pY-TrkA). The blot was stripped and reprobed with anti-pan-Trk antibody to demonstrate similar levels of TrkA protein before and after NGF treatment. (B) Down-regulation of Hu antigen in PC12 cells with reduced levels of Dnmt3b. Hu antigen was stained in the parental andvector-transfected cells and antisense Dnmt3b-transfected cells (#21 and #28) treated with NGF (50 ng/ml) for 6 days. The cells were then stained with antibody against Hu antigen ({alpha}-Hu), followed by TRITC-conjugated secondary antibody. DAPI was used for nuclear staining. Bar, 100 µm. (C) MAP2 expression is reduced in PC12 cells expressing antisense Dnmt3b. PC12 cells (parental, vector-transfected, and antisense Dnmt3b-transfected cells) were cultured with NGF (50 ng/ml) treatment for 6 days. Then, the cells were subjected to immunofluorescence analysis with Dnmt3b- and MAP2-specific antibodies. Subsequently, fluorescein isothiocyanate (FITC)- or TRITC-conjugated secondary antibodies were used for Dnmt3b and MAP2, respectively. DAPI was used to visualize the nucleus. Only one Dnmt3b antisense cell line is shown, since no significant MAP2 staining was observed in either number 21 or 28 cells. Bar, 250 µm.

 
Expression of neuronal-cell-specific markers, Hu antigen, and MAP2 is significantly diminished in cells expressing antisense Dnmt3b or treated with Dnmt3b-specific siRNA. Hu antigen (HuC and HuD) is expressed exclusively in nerve cells and is essential for initiating the differentiation of PC12 cells and maintaining neurite outgrowth (1, 17). To verify further that inhibition of Dnmt3b would hinder NGF-induced differentiation, we monitored Hu expression in stable cells expressing antisense Dnmt3b, as well as Dnmt3b siRNA-transfected PC12 cells, by immunofluorescence analysis. As expected, Prominent Hu antigen staining was observed in both parental and vector control cells after NGF-induced differentiation (Fig. 4B, panels a and b). However, the staining was significantly decreased in cell lines with reduced Dnmt3b (Fig. 4B, panels c and d). The decrease in Hu antigen expression was also observed in PC12 cells depleted of Dnmt3b with siRNA (data not shown). Hu antigen was not detected in all the cells before NGF treatment, which was consistent with the specific synthesis of Hu antigen in neuronal cells (data not shown). These results reinforce the earlier conclusion based on morphological studies (Fig. 2) that Dnmt3b depletion indeed inhibits NGF-induced PC12 cell differentiation.

We also monitored the expression of MAP2, another neuronal marker induced by NGF in PC12 cells (16). There was no significant MAP2 expression in untreated cells (data not shown). MAP2 expression was induced in the parental (Fig. 4C, panel a), as well as in vector-transfected cells after NGF treatment (panel d). It occurred concurrently with an increase in Dnmt3b expression after NGF treatment (Fig. 4C, panels b and e, and 1F). By contrast, MAP2 expression was significantly reduced in cells depleted of Dnmt3b (Fig. 4C, panels g and j). These results demonstrate the correlation between the expression of neuronal markers, Hu antigen, and MAP2 and the Dnmt3b levels in PC12 cells.

The methylation status of the repetitive elements is not significantly altered by inhibition of Dnmt3b expression. To elucidate the role of Dnmt3b in NGF-induced PC12 cell differentiation, we examined the potential alteration in the DNA methylation profile in Dnmt3b-depleted cells. The majority of the CpGs are distributed in repetitive elements of the entire genome. The methylation of these sequences is essential for the maintenance of genomic stability and chromatin integrity (14). IAP and Alu repeats are two major mobile repetitive elements frequently found in the genome. Active amplification and insertion of these repeats contribute to genomic instability (33, 47, 50). In addition, satellite 1 is a common repetitive element that constitutes the majority of the centromere and telomere of the rat chromosomes (28). To test if the methylation status of these repetitive elements was altered upon Dnmt3b depletion, genomic DNA was digested with the methylation-sensitive restriction enzyme HpaII or the isoschizomeric restriction enzyme MspI, which can cleave DNA regardless of the methylation status of the CpG. Southern blot analyses of these repetitive elements were performed to analyze the DNA methylation patterns of these regions. The results showed that genomic DNAs from the untreated and NGF-treated PC12 cells were resistant to HpaII digestion but were susceptible to MspI digestion (Fig. 5, lanes 7 to 9). Dnmt3b-depleted cells generated similar HpaII digestion profiles (lanes 3, 5, and 11). No significant changes were observed even after NGF treatment (lane 13). These results demonstrated that the methylation pattern in the genome of undifferentiated PC12 cells had already been established and did not change dramatically after NGF treatment. Depletion of Dnmt3b alone did not affect the methylation profiles of at least three repeat elements.



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  		FIG. 5. Absence of significant alteration in the methylation pattern of the IAP, AluI, and rat satellite 1 repetitive elements in Dnmt3b-depleted PC12 cells. Genomic DNAs (5 µg) isolated from untreated parental and 3bsi-transfected cells with (+) or without (–) NGF treatment, antisense clones 28 and 21, and the vector-transfected (Vector) cells were digested with the methylation-sensitive enzyme HpaII (H) or the methylation-insensitive enzyme MspI (M). The digested DNA was separated on an agarose gel (0.8%), transferred to a nitrocellulose membrane, and hybridized with 32P-labeled random-primed IAP, AluI, or rat satellite 1 probe, followed by autoradiography and phosphorimager analysis.

 
The N-terminal region of Dnmt3b that harbors the ATRX domain is required for NGF-dependent differentiation. As a first step to explore the underlying mechanism by which Dnmt3b regulates NGF-induced differentiation of PC12 cells, it was necessary to identify the domain of Dnmt3b that was essential for this process. Dnmt3b has a relatively large N-terminal domain and a C-terminal catalytic domain (Fig. 6A). The function of the N-terminal domain is largely unknown. The ATRX domain located in this region is homologous to the ATRX gene, encoding a member of the SWI/SNF family of chromatin-remodeling proteins (6). To identify the domain(s) required for the differentiation process, we generated N-terminal, the C-terminal, and the ATRX domain deletion mutants of Dnmt3b. In addition, a mouse wild-type Dnmt3b and a catalytic-site point mutant (C657S) that abolished DNA methyltransferase activity were used (30). Wild-type Dnmt3b and different Dnmt3b mutants were cotransfected with Dnmt3b siRNA to determine their abilities to recover NGF-induced differentiation in PC12 cells. This siRNA would induce degradation specifically of endogenous rat Dnmt3b but not transfected mouse Dnmt3b, as it was designed to target the 3' UTR of rat Dnmt3b and was absent in the mouse Dnmt3b cDNA expression vector (Fig. 6A). Overexpression of either the wild type or mutants of Dnmt3b did not significantly alter PC12 cell morphology in the absence of NGF (data not shown). In siRNA-treated cells, the wild-type Dnmt3b could recover NGF-induced neurite outgrowth by 90%. Moreover, the rescue of the neuronal phenotype could also be achieved by overexpression of the C657S mutant, which was catalytically inactive (30). Approximately 88% of the cells could grow neurites, compared to vector-transfected control cells in the presence of NGF.Vector-transfected cells generated a few neurites, which ruled out the possibility that this rescue was due to the introduction of exogenous DNA (Fig. 6B and C). These data suggest that the function(s), other than the methyltransferase activity, of Dnmt3b is required for mediating NGF-induced neuronal-cell differentiation.



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  		FIG. 6. The N-terminal domain of Dnmt3b restores NGF-induced PC12 cell differentiation. (A) Schematic representation of the different mutants of Dnmt3b used in the present study and Western blot analysis for analyzing their expression in PC12 cells. Anti-Myc antibody ({alpha}-myc) was used to detect Myc-tagged wild-type (WT) and catalytic-domain mutant Dnmt3b (C6547S), and anti-Flag antibody ({alpha}-flag) was used to show the overexpression of {Delta}ATRX, {Delta}C, and {Delta}NDnmt3b. Corresponding vectors were used as negative controls. The expected peptides are indicated (arrows). aa, amino acids. (B) Cotransfection of wild-type Dnmt3b or different mutant constructs, along with 3bsi. Cells were transfected with different DNA constructs (as indicated) with Lipofectamine reagent. After 24 h, the cells were treated with NGF and allowed to grow for an additional 6 days with two intermediate cotransfections every 72 h. Bar, 100 µm. (C) Ability of the wild type or different mutants of Dnmt3b to recover NGF-induced neurite outgrowth. The cells bearing neurites at least two cell bodies in length were counted. About 200 cells were counted in each sample. The error bars represent standard deviations of triplicate experiments (t test; P < 0.01). Con, control; CS, C657S. (D) Overexpression of N-terminal domain of Dnmt3b facilitates differentiation. (D-1) Quantitative analysis of cells generating neurites at least two cell bodies in length (t test; P < 0.01). d, day(s). (D-2) Microscopy images of vector-transfected and {Delta}CDnmt3b-transfected cells. Bar, 250 µm.

 
Next, we overexpressed the N-terminal domain of Dnmt3b that lacks the C-terminal catalytic domain ({Delta}C) in cells treated with Dnmt3b siRNA. {Delta}C restored NGF-induced differentiation to 70% of the level in vector-transfected cells. These data demonstrated that the catalytic-domain deletion mutant could compensate for the loss of endogenous Dnmt3b. In contrast, cells cotransfected with either {Delta}ATRX or {Delta}N, along with siRNA, grew in clusters, and most of the cells did not produce visible neurites. The morphology of these cells resembled that of the siRNA-depleted cells (Fig. 6B and C). To further evaluate the ability of the N-terminal domain alone to regulate NGF-induced PC12 cell differentiation, we compared the differentiation potentials of the cells stably transfected with the N-terminal domain ({Delta}Cdnmt3b) and of the vector-transfected cells. Overexpression of this domain also facilitated neurite outgrowth in these cells (Fig. 6D). At each time point studied during the course of differentiation, these cells differentiated more rapidly, in terms of both numbers of neurite-bearing cells and average neurite length, than the parental cells (P < 0.01) (Fig. 6D). Overexpression of the N-terminal domain and the wild-type Dnmt3b had a similar effect on NGF-induced PC12 cell differentiation (data not shown). Since NGF-induced PC12 cell differentiation could be recovered only in the presence of the N-terminal domain, a function(s) other than the catalytic activity of Dnmt3b must be required for differentiation into neuronal cells. Furthermore, the ATRX domain appears to be essential for this novel function(s).

Dnmt3b-associated histone deacetylase activity is elevated in PC12 cells upon NGF treatment, which is essential for neurite outgrowth. Apart from their DNA methyltransferase activities, Dnmt1, -3a, and -3b can also regulate gene expression by recruiting various chromatin-modifying factors, such as Hdacs (2, 20, 43). As the N-terminal domain of Dnmt3b that harbors the ATRX domain could restore NGF-induced differentiation in Dnmt3b-depleted cells, we raised the possibility that Dnmt3b could recruit Hdacs to facilitate the differentiation process. To address this issue, we first determined whether Hdac activity was critical for neurite outgrowth. Treatment of PC12 cells with NGF in the presence of the Hdac inhibitor TSA (50 nM) or depsipeptide (10 nM) generated only a few visible neurites, even after 5 days of NGF treatment, whereas extensive neurite outgrowth was observed in cells treated with NGF alone (P < 0.01) (Fig. 7A). This result suggests that Hdac activity is essential for NGF-induced differentiation of PC12 cells. None of these inhibitors alone promoted differentiation in PC12 cells in the absence of NGF (i.e., same as day zero of NGF treatment in Fig. 7A and data not shown).



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  		FIG. 7. The N-terminal domain of Dnmt3b recruits histone deacetylase and facilitates differentiation. (A) Impeding of NGF-induced PC12 cell differentiation by the histone deacetylase inhibitor TSA or depsipeptide. (A-1) Quantitative analysis of cells bearing visible neurites on different days (d) of NGF treatment (t test; P < 0.01). (A-2) Microscopic images of cells in the presence of vehicle (ethanol), TSA (50 nM), or depsipeptide (10 nM), along with NGF, after 5 days. Bar, 250 µm. (B) Analysis of Hdac activities in the immunoprecipitated protein complex pulled down by Dnmt3b antibody from undifferentiated and differentiated PC12 cells. An immunoprecipitation assay was performed with cell extracts from untreated (–) and NGF-treated (+) cells. The immunoprecipitated proteins pulled down were subjected to Hdac assay with 3H1-labeled acetyl histone H4 peptide. Butyrate was used to demonstrate that release of [3H1]acetate from acetyl-H4 beads was indeed due to Hdac. The error bars represent standard deviations of duplicate experiments with two different batches of extracts. (C) Higher Hdac activity pulled down by Dnmt3b antibody from cells overexpressing the N-terminal domain of Dnmt3b ({Delta}CDnmt3b). (C-1) Western blot analysis with Dnmt3b antibody was performed to show comparable levels of endogenous Dnmt3b in both vector- and {Delta}CDnmt3b-transfected cells and an additional 72-kDa polypeptide corresponding to the overexpressed protein in {Delta}CDnmt3b-transfected cells. WT, wild type. (C-2) Hdac activities pulled down by Dnmt3b antibody in the {Delta}CDnmt3b-overexpressing cells and vector-transfected cells. Butyrate was used to inhibit histone deacetylase activity. The error bars represent standard deviations of duplicate experiments. This experiment was repeated with different cell extracts.

 
Next, we tested whether Dnmt3b was associated with Hdac activity in PC12 cells. Immunoprecipitation analysis with Dnmt3b-specific antibody or preimmune serum was performed with cell lysates from NGF-treated and untreated PC12 cells. An Hdac assay with the immune-precipitated protein complex showed that Hdac activity was indeed associated with Dnmt3b. Moreover, this activity increased fourfold after NGF treatment (Fig. 7B). Inhibition of the deactetylation of 3H1-labeled acetyl-histone H4 peptide by sodium butyrate, a potent Hdac inhibitor, demonstrated that Hdac was indeed pulled down by anti-Dnmt3b antibody (Fig. 7B). Next, we measured Dnmt3b-associated Hdac activity in cells overexpressing the N-terminal domain ({Delta}C) of Dnmt3b that harbors the ATRX domain. Significantly higher Hdac activity (a 2.5-fold increase) was pulled down by anti-Dnmt3b antibody from these cells than from the control PC12 cells (Fig. 7C-2). The lack of alteration of the endogenous Dnmt3b protein level (Fig. 7C-1, upper bands) prompted us to conclude that the increased Hdac activity must be due to the overexpression of the N-terminal domain of Dnmt3b. These data suggest that the N-terminal domain recruits Hdac(s).

Dnmt3b preferentially interacts in PC12 cells with Hdac2, which is up-regulated upon NGF treatment. To identify the Hdac isoform associated with Dnmt3b in PC12 cells, we first studied the protein expression level of Hdac1 to -3 during NGF-induced differentiation. Western blot analysis showed that Hdac2 was up-regulated, whereas Hdac3 was down-regulated, during the differentiation process (Fig. 8A). Hdac1 could not be detected with the regular ECL detection system used for both Hdac2 and Hdac3. Very low levels of Hdac1 could be detected only with a detection system that was 5 to 10 times more sensitive, and its level was not significantly altered upon differentiation (Fig. 8A).



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  		FIG. 8. Association of Dnmt3b with Hdac2 in PC12 cells. (A) Up-regulation of Hdac2 in PC12 cells after differentiation. Whole-cell extracts (200 µg of protein) from untreated (–) and NGF-treated (+) cells (6 days) were used for Western blot analysis with Hdac2 antibody. The blot was stripped and reprobed with Hdac3 or Hdac1 antibody. Hdac1 could not be detected with a regular ECL Western blotting detection system (Amersham). A low level of Hdac1 was detected using highly sensitive chemiluminescent peroxidase substrate from Sigma. (B) Coimmunoprecipitation analysis. Whole-cell extracts (1 mg of protein) from PC12 cells treated with NGF for 6 days were immunoprecipitated with anti-Dnmt3b ({alpha}-Dnmt3b) or anti-Hdac2 ({alpha}-Hdac2) antibody. Dnmt3b preimmune serum and mouse IgG were used as respective negative controls. The precipitated proteins were resolved by SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to Western blot analysis with Dnmt3b and Hdac2 antibodies. (C) Cosedimentation of Dnmt3b with a population of Hdac2 during glycerol density gradient fractionation. Whole-cell extract from 107 PC12 cells (NGF treated for 6 days) was fractionated by glycerol density (5 to 30%) gradient. Fractions were collected from the bottom of the tube and separated on an SDS-PAGE gel, followed by Western blot analysis with Dnmt3a, Dnmt3b, and Hdac1 to -3 antibodies.

 
The up-regulation of both Dnmt3b and Hdac2 in NGF-treated PC12 cells suggested a possible interaction between the two proteins. To demonstrate the interaction between the two proteins in vivo, we performed immunoprecipitation with anti-Dnmt3b or anti-Hdac2 antibody using whole-cell extract from NGF-treated PC12 cells. The results of the coimmunoprecipitation studies demonstrated that either antibody could precipitate both Dnmt3b and Hdac2 (Fig. 8B). Normal mouse IgG or Dnmt3b preimmune serum did not pull down any of these proteins, which showed the specificity of the interaction between Dnmt3b and Hdac2 (Fig. 8B).

The association of Dnmt3b with Hdac2 was further confirmed by glycerol density gradient sedimentation of extract from NGF-treated PC12 cells, followed by Western blot analysis. The results showed that Dnmt3b comigrated with Hdac2, whereas Dnmt3a peaked at a higher density away from the fractions containing Dnmt3b and Hdac2 (Fig. 8C). Interestingly, Hdac1 cofractionated with Dnmt3a, which suggested their interaction in PC12 cells. In undifferentiated cells, Dnmt3b and Hdac2 profiles were very similar, but their levels were significantly reduced (data not shown). Hdac3 could be detected only in extracts from undifferentiated cells that migrated at a higher glycerol density (data not shown).


   DISCUSSION
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 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
The higher level of Dnmt3b in the neural ectoderm at embryonic stage E7.5 and the predominant expression of Dnmt3b in the forebrain and eye at a later stage of mouse embryo development (40) suggested an important role for this DNA methyltransferase in neural development. The present study was undertaken to test the hypothesis that Dnmt3b is crucial for neuronal differentiation. The results showed that Dnmt3b expression increased during NGF-induced neurite outgrowth in PC12 cells, and the cells depleted of Dnmt3b lost their ability to differentiate into the neuronal phenotype.

The methylation profile was established in undifferentiated PC12 cells, as revealed by the resistance of genomic DNA to HpaII digestion (Fig. 5). The methylation status of the repetitive elements was not significantly altered upon NGF treatment, which suggested that the methylation profile was maintained in differentiated cells. DNA methylation could be maintained in PC12 cells with a marked reduction in Dnmt3b expression. The lack of alteration in the methylation status of the PC12 genome after Dnmt3b depletion was further confirmed by a methyl acceptance assay that measures the methyl-CpG content of DNA. Genomic DNA isolated from the clones (numbers 28 and 21) or cells transfected with Dnmt3b siRNA, as well as the controls, was subjected to the methyl acceptance assay with [3H1]Ado-Met as a methyl donor (3). [3H1]methyl incorporation in PC12 cells was not significantly altered after NGF treatment (P > 0.85). Further treatment of cells with Dnmt3b siRNA, along with NGF, for 6 days did not change the global methylation status of the PC12 cell genome (data not shown).

To identify genes that were differentially methylated in PC12 cells after differentiation into neurites, we also performed restriction landmark genome scanning (RLGS) analysis with the methylation-sensitive enzyme NotI, a well-established technique for the analysis of the CpG methylation pattern of the whole genome. It differentiates genes based on the methylation status of the CpG island located predominantly in the promoter and exon 1 (15, 39). The RLGS profiles of at least 1,000 genes in PC12 cells before and after NGF treatment (for 14 days) were quite similar (data not shown). This observation further substantiates the conclusion, based on the methyl acceptance assay and Southern blot analysis of repetitive elements, that no significant changes in the methylation profiles of the genes with CpG islands occur during PC12 cell differentiation. Methylation-demethylation appears to be a long-term process requiring DNA replication and cell division. Indeed, erasure of the established genomic methylation profile in embryonic stem cells requires at least 40 passages even in Dnmt3a and Dnmt3b null cells (12). In contrast, differentiation was visible as early as 24 h after NGF treatment. These observations reinforce the conclusion that a nonenzymatic function of Dnmt3b plays a critical role in PC12 cell differentiation. Furthermore, Dnmt3b-mediated cell differentiation could be cell type specific, as Dnmt3b is dispensable for retinoic acid- or cyclic-AMP-induced F9 cell differentiation into ectoderm (data not shown). However, active changes in the methylation status can occur rapidly in the differentiation of specific cell types (19, 46, 52).

It is logical to assume that the up-regulation of Dnmt3b could compensate for the loss of Dnmt1 and Dnmt3a expression upon NGF-induced differentiation. Dnmt3a and Dnmt3b are known to exhibit some overlapping functions during mouse embryo development (40). Deletion of one gene does not significantly change the overall de novo methylation patterns, whereas genomewide hypomethylation occurs in Dnmt3a–/– Dnmt3b–/– mice (40). It is therefore conceivable that Dnmt3b accomplishes the overlapping functions when Dnmt3a is down-regulated. Surprisingly, DNA methylation could be maintained in PC12 cells with marked reduction in Dnmt3b expression, especially after NGF treatment. It is likely that Dnmt1 and Dnmt3a maintain the methylation profile in Dnmt3b-depleted cells prior to differentiation, and it remains unaltered after NGF treatment, as cells do not divide during differentiation. In this context, it is noteworthy that depletion of Dnmt3b alone in different human cancer cell lines does not alter the methylation profile of the genome (5). However, we cannot rule out changes in the methylation profiles of a few genes that could not be identified by RLGS analysis. These observations reinforce the conclusion that a nonenzymatic function independent of changes in the genomic DNA methylation pattern plays a critical role in PC12 cell differentiation. In this context, it is noteworthy that the noncatalytic domain of Dnmt3b could promote the suppression of the rRNA gene (S. Majumdar and S. Jacob, unpublished data).

Another contributing factor in neuronal differentiation could be the proteins associated with Dnmt3b. DNA methyltransferases are associated with Hdacs, the components of transcriptional repression complexes (20, 45). Treatment of cells with Hdac inhibitors alters the gene expression profile by hyperacetylation of N-terminal lysine residues of core histones, the basic proteins associated with nucleosomes (22, 32, 53). The N-terminal ATRX domain of Dnmt3a can be recruited to a specific promoter through its interaction with the gene-specific transcription factor Rp58 (20). In the present study, we have demonstrated that Hdac activity was associated with Dnmt3b in PC12 cells and that this activity was markedly altered after NGF treatment. Interestingly, overexpression of the ATRX-containing N-terminal domain of Dnmt3b resulted in higher levels of Hdac activity, indicating that the N-terminal domain of Dnmt3b indeed interacts with the histone deacetylases. Further study showed that Dnmt3b associates with Hdac2, whose expression was also up-regulated after NGF-induced differentiation. This finding is consistent with the report that Dnmt3b interacts with Hdac1 and Hdac2 in various cell types (21). Based on these observations, we propose that Dnmt3b functions as a transcriptional repressor by recruiting Hdac2 to the target gene promoters and that silencing these promoters is essential for neurite outgrowth. Identification of Dnmt3b target genes in PC12 cells will facilitate the elucidation of the function of Dnmt3b in NGF-induced differentiation.


   ACKNOWLEDGMENTS
 
We thank Chih-Lin Hsieh for providing the Myc-tagged wild type and catalytic-domain mutant of mouse Dnmt3b cDNA, En Li for mouse Dnmt3a and Dnmt3b cDNAs, Shoji Tajima for anti-Dnmt1 antibody, Arthur Burghes and Jill Rafael-Fortney for their generosity with the use of the fluorescence microscope, and Tasneem Motiwala and Jill Rafael-Fortney for critically reading the manuscript.

This study was supported in part by grants ES 10874, CA 81024, and CA 86978 (S.T.J.).


   FOOTNOTES
 
* Corresponding author. Mailing address: Department of Molecular and Cellular Biochemistry, College of Medicine, The Ohio State University, Columbus, OH 43210. Phone: (614) 688-5494. Fax: (614) 688-5600. E-mail: jacob.42{at}osu.edu. Back


   REFERENCES
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
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