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Genome Architecture of the Human ß-Globin Locus Affects Developmental Regulation of Gene Expression

Department of Biochemistry and Molecular Biology,¹ Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160,³ Division of Medical Genetics, University of Washington, Seattle, Washington 98195²

Received 16 January 2005/ Returned for modification 10 February 2005/ Accepted 21 July 2005

	ABSTRACT

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To test the role of gene order in globin gene expression, mutant human ß-globin locus yeast artificial chromosome constructs were used, each having one additional globin gene encoding a "marked" transcript (^m, ^m, or ß^m) integrated at different locations within the locus. When a ß^m-globin gene was placed between the locus control region (LCR) and the -globin gene, ß^m-globin expression dominated primitive and definitive erythropoiesis; only ß^m-globin mRNA was detected during the fetal and adult definitive stages of erythropoiesis. When an ^Am-globin gene was placed at the same location, ^Am-globin was expressed during embryonic erythropoiesis and the fetal liver stage of definitive erythropoiesis but was silenced during the adult stage. The downstream wild-type -globin genes were not expressed. When an ^m-globin gene was placed between the - and ß-globin genes, it remained silent during embryonic erythropoiesis; only the LCR-proximal wild-type -globin gene was expressed. Placement of a ß^m-globin gene upstream of the ^G-globin gene resulted in expression of ß^m-globin in embryonic cells and in a significant decrease in expression of the downstream wild-type ß-globin gene. These results indicate that distance from the LCR, an inherent property of spatial gene order, is a major determinant of temporal gene expression during development.

	INTRODUCTION

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The human ß-globin locus consists of five functional globin genes, which are expressed during ontogeny in the order in which they lie on the chromosome, 5'--^G-^A--ß-3'. During development, two switches occur in globin gene expression, from embryonic to fetal, coinciding with the transition from embryonic to definitive erythropoiesis, and from fetal to adult around the perinatal period. High-level expression of the ß-like globin genes is dependent upon both gene-proximal and -distal cis-acting regulatory elements. The major distal regulatory element is the locus control region (LCR). It contains five DNase I-hypersensitive sites (5'HS1 to -5) (42) and is located 6 to 22 kb upstream of the -globin gene. Functions of LCRs include tissue-specific enhancement of gene expression, chromatin-opening activity, insulation of the locus from the effects of surrounding chromatin, and determination of replication timing and choice of origin used (26, 27). It is currently assumed that LCR sequences, together with their cognate transcription factors and coactivators, form an active site "hub" that interacts with gene-proximal elements and their bound transcription factors to form a transcriptosome specific for the globin gene(s) expressed at each developmental stage (8, 20, 26, 34). Less-conserved sequences between the HS cores also may function in globin gene regulation (19), perhaps by acting to constrain the complex conformation.

Because the spatial arrangement of the ß-like globin genes parallels their temporal expression pattern, it has been proposed that gene order or proximity to the LCR may be a determinant of the order of globin gene expression during development. Previous studies have established an effect of either gene order or distance from the LCR (10, 18, 39, 44). Data from transgenic mice produced with - and ß-globin genes linked at various distances to the LCR demonstrated that proximity to the LCR increased the probability of interaction with the LCR (18). The effect of gene order on globin gene developmental regulation was tested by producing transgenic mice containing two tandemly arranged - or ß-globin or ß- and ß-globin genes linked to a µLCR (39). These studies showed that the trans-acting environment is the primary determinant for correct developmental control but that proximity to the LCR also is involved in regulation. The effect of distance from the LCR was analyzed using a larger 70-kb ß-globin locus in two linked cosmids (10). When the -globin gene was replaced with a marked ß-globin (ß^m) gene, ß-globin expression was sustained throughout development. In contrast, when the ß^m-globin gene was placed upstream of the -globin gene, ß^m-globin gene expression accounted for 75% of total ß-globin gene expression (ß^m + ß^wt). Inversion of the whole locus relative to the LCR in ß-globin locus yeast artificial chromosome (ß-YAC) transgenic mice resulted in ß-globin gene expression throughout development, with no -globin gene expression and limited -globin gene expression during primitive erythropoiesis, again suggesting that proximity to the LCR is a determinant of globin gene expression (44).

In this study we sought to further test the role of spatial gene order in the control of globin gene expression using ß-YAC transgenic mice carrying constructs containing one additional globin gene encoding a "marked" transcript (^m, ^m, or ß^m) integrated at a different locations within the locus. Our results indicate that distance from the LCR as determined by gene order, in addition to gene-proximal cis-acting elements, determines the appropriate developmental expression of ß-like globin genes.

	MATERIALS AND METHODS

Top Abstract Introduction Materials and Methods Results Discussion References

 
YAC constructs. A 213-kb ß-YAC containing the human ß-globin locus (Fig. 1) was utilized for all experiments (16, 36, 38). This ß-YAC was previously estimated to be 248 kb, but with a nearly complete sequence of the human ß-globin locus region now available (http://globin.cse.psu.edu) (4), the size was more accurately determined. An approximately 6-kb sequence gap still exists downstream of 3'HS1 in the latest release of the human genome sequence. The numerical coordinates used in construct descriptions below are based upon GenBank file U01317 unless indicated otherwise. All of the YAC modifications were introduced by yeast integrating plasmid (YIP)-mediated "pop-in," "pop-out" or two-step gene replacement methods of homologous recombination as previously described (1, 31, 38). Briefly, for the "pop-in," "pop-out" method, YIP clone DNA was linearized with the indicated restriction enzyme and transformed into spheroplasted Saccharomyces cerevisiae strain AB1380 containing the 213-kb ß-YAC. Transformants were selected for uracil prototrophy on complete medium (CM), and correct recombination was determined by Southern blot hybridization analysis. Spontaneous excision of the YIP was induced by overnight growth in nonselective CM. The yeast cells were plated on 5-fluoroorotic acid (5-FOA) CM plates to select for loss of the URA3 gene residing on the YIP vector, which results in 5-FOA resistance. The two-step gene replacement protocol utilized the same selection steps as the "pop-in," "pop-out" method, except two fragments with recombinogenic ends were used in sequential transformations. The first fragment contained the target sequence and the marked globin gene interrupted with the URA3 cassette; the second fragment was identical, but it lacked the URA3 gene. Correct recombinants were confirmed by Southern blot hybridization analysis and PCR.

View larger version (7K): [in this window] [in a new window]   FIG. 1. Gene order constructs. The human ß-globin locus is shown as a line, the genes are indicated as rectangles, and the DNase I-hypersensitive sites are marked with arrows. The various marked globin genes and their sites of integration are shown with arrows linking them to the site of integration.

ß^m 5' ß-YAC.

ß^m 5' ^G ß-YAC. A 4.1-kb HpaI-XbaI ß^m fragment was inserted 5' of the ^G-globin gene into a BglII site at GenBank coordinate 32837 (Fig. 1). A 3,167-bp XbaI fragment 5' to the ^G-globin gene (GenBank coordinates 32011 to 35178) was purified from pA-36 (gift from B. Forget) and ligated into the XbaI-digested and phosphatase-treated YIP vector pRS406 (Stratagene, La Jolla, CA) to produce pRS-5' ^G. The 4.1-kb HpaI-XbaI ß^m-globin fragment was prepared as described in the preceding section and ligated into BglII-cut, Klenow enzyme-filled and phosphatase-treated pRS-5' ^G to produce pRS ß^m 5' ^G. This YIP was linearized with Tth111I prior to transformation of ß-YAC-bearing yeast. Differential analysis of ß^m- and ß^wt-globin transcripts in murine lines was as described in the preceding section for the ß^m 5' ß-YAC construct.

^Am 5' ß-YAC. A 5.4-kb ^Am-globin SspI fragment (GenBank coordinates 38683 to 44077) was inserted at the same EcoRV site described for the ß^m 5' ß-YAC construct (Fig. 1). The ^Am-globin gene contains a six-base-pair deletion at +21 to +26 relative to the ^A-globin translation start site (41). A 3,139-bp BglII-HindIII fragment (GenBank coordinates 13769 to 16908) was isolated from pHSI and ligated into BamHI-HindIII-cut and calf intestinal alkaline phosphatase-treated pBluescript KS+ (Stratagene, La Jolla, CA) to produce pBS-5' . The 5.4-kb SspI-SalI fragment encompassing the ^Am-globin gene was isolated from pUC19 ^Am (+) (39) and ligated into EcoRV-digested and phosphatase-treated pBS-5' to produce pBS ^Am 5' . An 8.5-kb SalI-NotI fragment encompassing the ^Am 5' insert was isolated from pBS ^Am 5' and subcloned into SalI-NotI-digested and phosphatase-treated pRS406 to produce pRS ^Am 5' . This YIP construct was linearized with PshAI (GenBank coordinate 16300) prior to transformation of yeast and recombination into the ß-YAC. Differential analysis of ^Am- and ^Awt-globin transcripts in transgenic mouse lines was as described for the ß^m 5' ß-YAC construct.

^m 3' ß-YAC. A 3.7-kb EcoRI ^m-globin fragment (GenBank coordinates 17482 to 21233) was inserted between the - and ß-globin genes in a HindIII site at GenBank coordinate 59641 (Fig. 1). The mark is an eight-base-pair XhoI linker inserted in a PvuII site at GenBank coordinate 19524 (Q. Li, personal communication). A 2,595-bp EcoRI fragment encompassing sequence 3' to the -globin gene (GenBank coordinates 58034 to 60629) was isolated from p BglII and ligated into EcoRI-digested and phosphatase-treated pUC19, in which the HindIII site had been previously ablated by digestion with HindIII, Klenow enzyme filling of the 5' overhangs, and religation to generate pUC-3' . A 3,770-bp EcoRI fragment encompassing the -globin gene was isolated from p3.7 (2), Klenow enzyme filled, and ligated in HindIII-cut, Klenow enzyme-filled and phosphatase-treated pUC-3' to produce pUC 3' . A 1,516-bp ClaI-SpeI fragment encompassing the -globin mark was purified from p3.7^m (K. R. Peterson, unpublished construct) and ligated into ClaI-SpeI-cut and phosphatase-treated pUC 3' to generate pUC ^m 3' . The 6,370-bp EcoRI fragment containing the entire insert from pUC ^m 3' was subcloned into EcoRI-digested and phosphatase-treated pRS406 to produce pRS ^m 3' . A PmlI site (GenBank coordinate 60366) was used to linearize this YIP vector prior to transformation of yeast and "pop-in," "pop-out" recombination. Measurement of ^m- and ^wt-globin transcripts in transgenic mice was as described above for the ß^m 5' ß-YAC construct.

YAC purification and transgenesis. Purification of ß-YAC DNAs for microinjection was performed as described previously (16, 35-38). Briefly, pulsed-field gel electrophoresis of preparative high-mass DNA agarose plugs was used to fractionate yeast chromosomes and the ß-YAC, followed by electrophoretic concentration of the YAC DNA in a 4% low-melting-point agarose gel, ß-agarase digestion of the concentrated YAC DNA plugs, and filtration of the liquefied agarose solution through a 0.22-µm syringe filter in high-salt microinjection buffer. Purified and filtered YAC DNAs were microinjected into fertilized mouse oocytes (C57/Bl6) and then transferred to pseudopregnant foster mothers to produce transgenic mice. Transgenic animals were identified by PCR analysis of tail biopsy DNA. Founders were bred with nontransgenic mice to produce F₁ progeny; these in turn were bred to obtain F₂ staged embryos, fetuses, and adults. All structure-function analyses were carried out beginning with the F₂ generation.

Structural analysis of transgene integrity and copy number determination. Structural analysis of ß-YAC transgenes was performed to assess the integrity and colinearity of transgenes as described previously (31, 38). High-molecular-weight DNA embedded in agarose was prepared, and slices were digested with SfiI. Digested DNA was fractionated by pulsed-field gel electrophoresis and transferred to a Zeta-probe charged nylon membrane (Bio-Rad, Hercules, CA) by capillary blotting. Strips representing individual lanes of the gel were cut from the blot, and each one was hybridized with one of the following ³²P-radiolabeled probes: 0.7-kb PstI 5'HS3, 1.9-kb HindIII 5'HS2, 1.8-kb XbaI 5'HS1, 3.7-kb EcoRI -globin gene, 2.4-kb EcoRI fragment 3' ^A-globin gene, 1.0-kb EcoRV ß region, 2.1-kb PstI fragment 5' -globin gene, 0.9-kb EcoRI-BamHI fragment 3' ß-globin gene, 1.4-kb XbaI DF10 (3'HS1), 1.9-kb BglII HPFH3, 0.5-kb HindIII H500, and 1.5-kb EcoRI-BglII HPFH6. DNA probes were labeled using the Decaprime II kit (Ambion, Austin, TX), following the manufacturer's instructions.

The presence of 5'HS4 and 5'HS5 was confirmed by Southern blot hybridization using a ³²P-radiolabeled 3.2-kb BamHI fragment (Globin Gene Server human ß-globin locus coordinates 215110 to 218333) as a probe (4). Digestion with SfiI (218055) and MluI (226095) produces a diagnostic 8.0-kb fragment for 5'HS5 and 5'HS4. All lines contained 5'HS4 and -5 unless otherwise indicated.

Transgene copy numbers were determined by Southern blot hybridization using LCR 5'HS3, -, and ß-globin gene fragment probes coradiolabeled with a mouse Thy 1.1 gene fragment as described previously (15, 30). Probe-specific activity was corrected, and comparison of hybridization signal intensity between human globin transgenes and the endogenous diploid murine Thy1.1 gene gave an estimate of transgene copy number. Radioactive signals were quantitated using a phosphorimager (Packard Instruments, Meriden, CT).

RPA. Total RNA was isolated from yolk sac, fetal liver, and blood of developmentally staged transgenic F₂, or later, embryos, fetuses, and adults by the method of Chomczynski and Sacchi (7) using the RNAgents total RNA isolation reagents (Promega, Madison, WI) as previously described (39). Human and murine globin mRNAs were detected by RNase protections performed as described previously (21, 39). Antisense RNA probes were prepared using the MaxiScript II kit (Ambion, Austin, TX). Template DNAs used to prepare riboprobes to measure endogenous mouse - and -globins were pT7Mo and pT7Mo, respectively (28), and to measure human -, -, and ß-globins, the templates were pT7Hu(188), pT7^Am(170), and pT7ß^m, respectively (25, 43). Individual human ß-like globin gene expression levels were quantitated as percentages of total human ß-like globin gene expression or as percentages of murine - and -globin gene expression corrected for transgene and endogenous murine gene copy number using data collected on a Cyclone phosphorimager and OptiQuant analysis software (Packard Instruments, Meriden, CT). RPAs were done separately with each conceptus of each litter to minimize experimental error and determine variation in globin gene expression. For each construct, three transgenic lines were established, and with rare exceptions, two or three conceptuses were analyzed at each developmental stage. Quantitative data include the means and standard deviations from two or three experiments for each line. Thus, averages at each developmental stage are indicative of 4 to 9 experimental values for individual transgenic lines and 12 to 27 values for combined lines. Wild-type ß-YAC lines 4 and 10 served as controls for RPA and RT-PCR RFLP (40). All experiments performed included a parallel set of wild-type ß-YAC samples (data not shown). In all instances the outcome confirmed the data we have compiled in published or unpublished form over the past 12 years regarding expression levels in these lines. Wild-type ß-YAC control lanes were included in each figure for comparison purposes, for size or band location indicators only, or to demonstrate proper function of riboprobes. For statistical analysis, the Student t test of comparison of means was performed, and differences were considered significant at P values of <0.025.

Semiquantitative RT-PCR. cDNA was synthesized from 10-day embryonic yolk sac, 12-day fetal liver, 14-day fetal liver, or adult mouse blood total RNA using an oligo-dT primer (Promega Corp., Madison, WI) and Superscriptase II reverse transcriptase (Invitrogen, Carlsbad, CA). One microgram of total RNA was mixed with 0.5 µg oligo-dT and sterile water in a final volume of 11 µl. The mixture was heated to 70°C for 10 min and then chilled on ice. Once cooled, 4 µl 5x first-strand buffer, 2 µl 0.1 M dithiothreitol, 1 µl 10 mM deoxynucleoside triphosphate mix, and 1 µl RNasin (Promega Corp, Madison, WI) were added and the reaction mixture was heated at 42°C for 2 min. One µl Superscript II RT was added, and the 20-µl reaction mixture was incubated at 42°C for 50 min. RT enzyme was heat inactivated by incubation for 15 min at 70°C.

PCR was performed essentially as described by Omori et al. (32) using Biolase Taq polymerase (Bioline USA, Randolph, MA) in a 25-µl reaction mix containing 1x NH₄ buffer [16 mM (NH₄)₂SO₄, 67 mM Tris-HCl (pH 8.8), 0.01% Tween-20], 2 mM MgCl₂, 100 µM deoxynucleoside triphosphates, 1 µCi [-³²P]dCTP, 0.4 pmol forward primer, 0.4 pmol reverse primer, 1 U Taq polymerase, and globin gene-specific primers (Invitrogen, Carlsbad, CA). All primers were designed to cross an intron so that RNA-templated reverse-transcribed DNA amplification products could be distinguished from gene amplification products. For human ß-globin and mouse -globin, initial denaturation was at 95°C for 5 min, followed by 18 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 45 s; final extension was at 72°C for 10 min. Separate reactions were performed for human ß-globin and mouse -globin. Parallel titration reactions showed that this cycle number was within the exponential amplification range. The primer sequences for RT-PCR were as follows: ß^m 5' ß-YAC or ß^m 5' ^G ß-YAC ß-globin forward, 5'-ACATTTGCTTCTGACACAACTG-3', and reverse, 5'-AGGAGCCTGAAGTTCTCAG-3'. The mouse -globin primers employed were as described previously (32). A similar scheme was used for the human -globin gene products. Amplification conditions were identical to those used for ß-globin. The primer sequences for RT-PCR were as follows: ^m 3' ß-YAC forward, 5'-CATATCTGCTTCCGACACAG-3', and reverse, 5'-GGGGTAAACAACGAGGAGTC-3'. Mouse -globin was included as a control in separate reactions.

A nonquantitative RT-PCR was employed for the -globins. Initial denaturation was at 95°C for 5 min, followed by 20 to 40 cycles of 1-min steps at 95°C, 60°C, and 72°C; final extension was at 72°C for 10 min. The primer sequences for RT-PCR were as follows: ^Am 5' ß-YAC -globin forward, 5'-ACACTCGCTTCTGGAACGT-3', and reverse, 5'-TAGACAACCAGGAGCCTTCC-3'.

RT-PCR RFLP analysis. RT-PCR primers were designed to amplify regions of the wild-type and marked globin gene products that were distinguished by RFLP analysis. Following semiquantitative RT-PCR, 5 µl of the RT-PCR products were digested overnight with restriction enzymes and fractionated on an 8% native polyacrylamide gel, which was dried and subjected to phosphorimager analysis (Typhoon, GE Healthcare, Waukesha, WI). In the ß^m-globin gene constructs, the normal NcoI site was replaced with a ClaI site by virtue of introduction of the mark. Thus, the ß^wt-globin product was digested with NcoI but not ClaI, and the converse was true for the ß^m-globin product. For the ß^m 5' ß-YAC and ß^m 5' ^G ß-YAC mice, the full-length PCR product was 370 bp. Digestion of the wild-type product with NcoI produced 320-bp plus 50-bp bands, whereas the ß^m-globin product was not digested. ClaI digested the ß^m-globin product but not the ß^wt-globin product to produce 324-bp plus 50-bp fragments. All experiments were run twice using the samples indicated in the figures.

The ^m 3' ß-YAC samples were similarly processed, except that XhoI was used to distinguish the ^wt- and ^m-globin RT-PCR products. The 164-bp ^wt-globin product was not digested with XhoI, whereas the 172-bp ^m-globin product was cut into 155-bp and 17-bp fragments.

For the ^Am-globin construct, following RT-PCR, portions of the PCR product were subjected to overnight digestion with appropriate restriction enzymes, fractionated on a 2 to 3% agarose gel, and visualized by ethidium bromide staining. DdeI digestion was used to analyze the ^Am- and ^Awt-globin gene products. The ^Awt-globin RT-PCR product from ^Am 5' ß-YAC mice was cut with DdeI into 130-bp plus 19-bp fragments, whereas the ^Am-globin RT-PCR product remained as the full-length 159-bp fragment.

	RESULTS

Top Abstract Introduction Materials and Methods Results Discussion References

 
The goal of our studies was to analyze the effect of gene order on the temporal expression of the human ß-like globins using ß-YAC transgenic mice. Towards this purpose, four modified ß-YAC constructs were made (Fig. 1). Each of the constructs contained an additional marked ß-like globin gene inserted in a nonnative location within a 213-kb ß-YAC. For the construct ß^m 5' ß-YAC, a ß^m-globin gene was inserted 1.6 kb 3' to 5'HS1 and 4.4 kb 5' to the -globin gene CAP site. The ß^m 5' ^G ß-YAC construct bore a ß^m-globin gene inserted 1.7 kb 5' to the ^G-globin CAP site. For the ^Am 5' ß-YAC construct, an ^Am-globin gene was inserted at the same location as the ß^m-globin gene in the ß^m 5' ß-YAC construct. The ^m 3' ß-YAC construct contained an ^m-globin gene inserted 4.3 kb 3' to -globin exon 2 and 2.5 kb 5' to the ß-globin CAP site.

An adult ß-globin gene located proximally to the LCR is preferentially expressed throughout development. Three ß^m 5' ß-YAC transgenic lines were established. Each line harbored at least one intact human ß-globin locus transgene extending from 5'HS5 through the ß-globin gene enhancer (see Fig. S1 in the supplemental material). Expression studies were done by RNase protection assay using embryonic, fetal, and adult erythroid cells of F₂ progeny of the transgenic lines.

As shown in Fig. 2, the ß^m-globin gene was expressed throughout development, beginning as early as detectable (day 8) and continuing through adulthood. -Globin mRNA was not detected and -globin gene expression was reduced during embryonic erythropoiesis. At day 10 postconception, the -globin level was 15% of total human globin expression [/(+ß)] versus 90% in wild-type ß-YAC transgenic mice [/(+)].

View larger version (126K): [in this window] [in a new window]   FIG. 2. Developmental profile of ß-like globin gene expression in ßm 5' ß-YAC transgenic mice. Total RNA was isolated from the blood of staged fetuses and subjected to RNase protection analysis. Representative data from line 1 are shown. The days after conception are shown at the top of the autoradiograph. The numbers indicate individuals of the same developmental stage. Antisense RNA probes for human -, -, and ß-globin mRNAs were utilized. Protected fragments are displayed on the right. The protected fragment sizes are as follows: human ß-globin exon 2 (ß ex 2), 205 bp; -globin exon 2 (), 188 bp; -globin exon 2 (), 170 bp; marked ß-globin exon 1 (ßm ex 1), 145 bp; wild-type ß-globin exon 1 (ßwt ex 1), 91 and 50 bp. The ß-globin antisense probe hybridizes with exons 1 and 2 of both the ßwt- and ßm-globin mRNAs. Thus, the exon 2 protected fragment is the sum of these two gene products, and the exon 1 protected fragments distinguish the two mRNAs. The marker (M) in the left lane is a radiolabeled MspI digest of plasmid pBR322; fragment sizes are shown in base pairs. FL, fetal liver; Bl, blood; wt ß-YAC, wild-type 213-kb human ß-globin locus YAC transgenic mouse line 4 (42). Lines 2 and 3 showed an identical phenotype.

Semiquantitative RT-PCR coupled with restriction enzyme digestion analysis (RT-PCR RFLP) was performed using primers for the ß-globin 5' untranslated region (UTR) and exon 1/exon 2 boundary. cDNAs were synthesized from adult blood RNA and amplified by PCR, and the PCR products were digested overnight with restriction enzymes ClaI or NcoI. These data confirmed that only the ß^m-globin gene was expressed in adult mice carrying the ß^m 5' ß-YAC construct (Fig. 3). This analysis also demonstrated that the wild-type ß-globin gene was not expressed at any stage of development. ClaI treatment showed complete digestion of the 370-bp fragment, whereas NcoI did not digest the PCR product at all, demonstrating that all of the ß-globin mRNA synthesis in these lines was ß^m-globin. Furthermore, ß^wt-globin protected fragments were not observed in Fig. 2, nor were they observed in RNase protection assays using only a ß-globin exon 1 antisense riboprobe to exclusively detect and distinguish ß^m- and ß^wt-globin mRNAs (data not shown).

View larger version (57K): [in this window] [in a new window]   FIG. 3. Semiquantitative RT-PCR RFLP analysis of ß-globin expression in ßm 5' ß-YAC transgenic mouse lines. RT-PCRs were performed to amplify the 5' end of the ß-globin transcript in adult mouse blood total RNA samples from ßm 5' ß-YAC lines 1, 2, and 3 (See Materials and Methods). Representative data from two adult individuals for each line are shown. The 4-bp insertion in the ßm-globin gene created a diagnostic ClaI restriction enzyme site and destroyed an NcoI site. ClaI digestion of the ßm-globin produces 324-bp and 50-bp fragments, whereas NcoI does not cut (ßm control lane). The reverse occurs for wild-type ß-globin; NcoI produces 320-bp and 50-bp fragments, whereas ClaI does not cut (ßwt control lane). Both enzymes digest proportionately to gene product levels as shown in the ßmßwt control lane, where the majority of expression in these µLCRßmßwt mice is ßm-globin. Top panel, controls: ßmßwt, µLCRßmßwt transgenic mouse adult blood (39); ßm, µLCRßmA transgenic mouse adult blood (39); ßwt, wild-type 213-kb ß-YAC transgenic mouse line 4 adult blood (40). Bottom panel: ßm 5' ß-YAC lines 1, 2, and 3. Digest fragment sizes are shown on the left, along with the location and size of the mouse -globin product used to normalize our data. Molecular weight marker (M) sizes are listed on the right side of the panels.

Representative results by RNase protection assay are shown in Fig. 4. ß^m-Globin expression was detected in the embryonic yolk sac, and expression continued through definitive erythropoiesis in both fetal liver and adult blood (Fig. 4B). Nearly all of the ß-globin expressed was ß^m-globin, although ß^wt-globin expression was detectable in day 14 fetal liver and adult blood by RPA using only a ß-globin antisense riboprobe to reduce background (Fig. 4C). In the full-length ß-YAC mice (lines 1 and 2), mean -globin expression in the day 10 yolk sac was 2.5-fold higher than in wild-type ß-YAC lines (P < 0.01), whereas -globin expression was 45% lower than in control mice (P < 0.01) (Table 1; Fig. 5A). During day 12 and 14 fetal definitive erythropoiesis, mean -globin expression was approximately 28% of the wild-type ß-YAC expression level (Fig. 5A; Table 1).

View larger version (67K): [in this window] [in a new window]   FIG. 4. Globin gene expression during development in ßm 5' G ß-YAC transgenic mice. RPAs of samples from line 1 are shown. (A) Human ß-like globin expression in blood. (B) Expression of human and murine globin genes in hematopoietic tissues. (C) ßm- versus ßwt-globin mRNA expression in blood. Labeling is as for Fig. 2. Developmental days are indicated above the images; ß-like globin gene fragments are indicated on the right or left side, as are markers. The protected fragment sizes are the same as for Fig. 2 with the addition of mouse -globin (Mo ), 128 bp, and mouse -globin (Mo ), 151-bp doublet. YS, yolk sac; +, wild-type 213-kb ß-YAC transgenic mouse line 4 (40) positive controls.

View this table: [in this window] [in a new window]   TABLE 1. Human globin mRNA levels per copy of transgene and copy of endogenous murine - and -globin in ßm 5' G ß-YAC transgenic mice and wild-type ß-YAC control mice

View larger version (22K): [in this window] [in a new window]   FIG. 5. Human - and ß-globin mRNA levels in ßm 5' G ß-YAC transgenic mice during embryonic and definitive erythropoiesis. - or ß-globin gene expression levels were quantitated from RNase protection analyses of RNAs derived from the indicated tissues at the developmental stages shown by phosphorimaging. Levels were calculated as percentages of murine - and -globin gene expression corrected for transgene and endogenous murine gene copy number. The average and standard deviation for all individuals within all lines are plotted on the x axis. The number of conceptuses tested for each genotype is indicated in Materials and Methods; the range was from 9 to 27. (A) -Globin mRNA expression. (B) ß-Globin mRNA expression. Black bars, full-length ßm 5' G ß-YAC lines 1 and 2; hatched bars, ßm 5' G ßwt ß-YAC line 3; white bars, wild-type ß-YAC line 4 (40). Other abbreviations are as described in previous figure legends.

View larger version (57K): [in this window] [in a new window]   FIG. 6. Semiquantitative RT-PCR RFLP analysis of ß-globin gene expression in ßm 5' G ß-YAC transgenic mice. RT-PCRs were performed for wild-type ß-YAC and three ßm 5' G ß-YAC mouse lines. Total mRNAs from 10 day embryonic yolk sac (lanes 1), 12-day fetal liver (lanes 2), 14-day fetal liver (lanes 3), and adult blood (lanes 4) were analyzed using primers for the 5' UTR and exon 1/exon 2 boundary of the ß-globin gene (See Materials and Methods). The full-length product is 370 bp. Digestion of the wild-type ß-globin product with NcoI produces 320-bp and 50-bp fragments, whereas the ßm-globin product is not digested. ClaI digests the ßm-globin product, but not the ßwt-globin product, to produce 324-bp and 50-bp fragments. Top panel: left, wild-type 213-kb ß-YAC transgenic mouse line 4 (Wild-type ß-YAC [40]); right, ßm 5' G ß-YAC mouse line 1 (Line 1). Bottom panel: left, ßm 5' G ß-YAC line 2 (Line 2); right, ßm 5' G ßwt-globin ß-YAC mouse line 3, which bears a deletion of the normally located wild-type ß-globin gene [Line 3 (ßwt)]. NcoI completely digested the ßwt-globin product, whereas ClaI did not, in the wild-type ß-YAC mouse line. ClaI digested the ßm-globin PCR product, whereas NcoI did not, in ßm 5' G ßwt-globin ß-YAC line 3.

Together, these data suggest that the LCR-proximal ß^m-globin gene is expressed exclusively early in development (Fig. 4 and 6), and low-level expression of the more distal ß^wt-globin gene occurs beginning on day 12. Although there was a decline in ß^m-globin gene expression during adult erythropoiesis, the ß^m-globin gene was never completely silenced in spite of its location in the -globin gene domain. The elevated -globin gene expression during embryonic erythropoiesis suggests that location of the ß^m-globin gene 5' to the ^G-globin gene may give the -globin gene an expression advantage by downregulating -globin expression, especially in the absence of the ß^wt-globin gene. In day 12 fetal erythropoiesis, no -globin was observed in line 3 (ß^wt) mice, whereas in lines 1 and 2, -globin expression gradually declined, suggesting that the distal ß^wt-globin gene may exert a repressive effect on -globin expression.

A fetal -globin gene located proximally to the LCR is autonomously silenced during adult definitive erythropoiesis. Five ^Am 5' ß-YAC transgenic mouse lines were established; each contained at least one intact copy of the ß-YAC through the -globin genes (see Fig. S3 in the supplemental material). Three lines (1 to 3) carried at least one ß-YAC extending from 5'HS3 through the ß-globin gene, and two lines had deletions of the - and ß-globin genes and downstream sequences (ß line 1 and ß line 2). Representative results of RNase protection assays are shown for line 3 (Fig. 7). - and ^wt-globins were not expressed at any time during ontogeny. ^Am-Globin expression was initiated during primitive erythropoiesis and continued through fetal definitive erythropoiesis, as indicated by the presence of a 138-bp ^Am-globin exon 1 band. ^Am-Globin expression was not detected during adult definitive erythropoiesis in blood. ß-Globin was expressed during fetal definitive erythropoiesis and continued throughout adulthood. Analysis of lines lacking the ß^wt-globin gene also demonstrated that -, ^G-, and ^Awt-globins were not expressed at any stage (see Fig. S3 in the supplemental material, ß lines 1 and 2; data not shown). Interestingly, ^Am-globin expression was down-regulated even in the absence of the ß-globin gene, suggesting that the 5.4-kb SspI fragment carrying the ^Am-globin gene contains a silencer region. In addition, these data demonstrate that -globin expression may be autonomously silenced, independently from competition with the ß-globin gene for interaction with the LCR.

View larger version (60K): [in this window] [in a new window]   FIG. 7. Am-Globin is silenced in adult Am 5' ß-YAC transgenic mice. RPAs of developmentally staged fetuses from Am 5' ß-YAC line 3. (A) Human ß-like globin expression in blood. (B) Human and murine globin gene expression in hematopoietic tissues. Labeling is as for Fig. 2 and 4 with the addition of human Am-globin exon 1 (Am ex 1), 138 bp, and G/Awt-globin exon 1 (G/Awt ex 1), 118 bp. The A-globin antisense probe hybridizes with exon 2 of the Awt-, Am-, these and G-globin mRNAs; it also hybridizes with exon 1 of mRNAs. Thus, the exon 2 protected fragment is the sum of these three gene products, and the exon 1 protected fragments distinguish Am-globin mRNA from G- and Awt-globin mRNAs.

- and ß-globin gene expression was quantitated and compared to expression in wild-type ß-YAC mice (Table 2). In all lines, mean -globin expression was approximately the same as that measured in wild-type ß-YAC mice during primitive erythropoiesis, but expression varied widely between the mutant lines. Similarly, during fetal definitive erythropoiesis, -globin expression was variable between the different lines. The difference between the mutant and wild-type ß-YAC lines in day 12 fetal liver erythropoiesis (40% versus 24%, respectively) is not statistically significant. -Globin expression was essentially the same in mutant and wild-type mice by day 14, and it was absent in adult definitive erythropoiesis. ß-Globin was not detected during primitive erythropoiesis in either ^Am 5' ß-YAC mice or wild-type ß-YAC mice. In day 12 and day 14 fetal liver, ß-globin in the mutant line was 14% and 11% of the wild-type ß-YAC level, respectively, reflecting delayed activation of ß-globin gene transcription. During adult definitive erythropoiesis, mean ß-globin expression was 61% of that measured in wild-type ß-YAC mice. Analysis of the globin gene expression switching profile in blood confirmed that the switch from - to ß-globin expression was delayed compared to results for wild-type ß-YAC mice and occurred on approximately day 16 instead of on day 12 (data not shown).

View this table: [in this window] [in a new window]   TABLE 2. Human globin mRNA levels per copy of transgene and copy of endogenous murine - and -globin in Am 5' ß-YAC transgenic mice and wild-type ß-YAC control mice

An embryonic globin gene inserted between two adult globin genes is not expressed. Three ^m 3' ß-YAC lines were obtained (see Fig. S4 in the supplemental material). Analysis of -globin gene expression revealed that only wild-type -globin was expressed during primitive erythropoiesis; no signal was observed from the ^m-globin gene at any stage of development (Fig. 8A). These data were confirmed by semiquantitative RT-PCR RFLP analysis as described in Materials and Methods (Fig. 9). ^wt-Globin synthesis detected in day 12 and 14 fetal liver is due to the presence of primitive erythrocytes from earlier yolk sac erythropoiesis in the fetal circulation (31). Although transcription was not observed, the integrity of the inserted ^m-globin gene was confirmed by Southern blot hybridization and PCR analyses (see Fig. S4 in the supplemental material; also data not shown). Expression of the gene was confirmed by RPA of erythroid cells containing an ^m-globin plasmid (data not shown). The insertion of an ^m-globin gene between the - and ß-globin genes had no effect on either the temporal or spatial pattern of globin gene expression within the locus (Fig. 8B). More recently, a derivative of the ^m 3' ß-YAC construct was synthesized that lacks the normally located ^wt-globin gene. Transgenic lines produced from this construct also did not display ^m-globin gene expression at any stage of development as measured by semiquantitative RT-PCR (data not shown).

View larger version (91K): [in this window] [in a new window]   FIG. 8. m-Globin is not expressed in m 3' ß-YAC transgenic mice. RPAs of developmentally staged fetuses from m 3' ß-YAC lines. Labeling is as for Fig. 2, 4, and 7. (A) Wild-type -globin mRNA is detected as a 127-bp exon 1 protected fragment, whereas m-globin mRNA is detected as a 152-bp exon 1 protected fragment. (B) The temporal pattern of globin transgene expression.

View larger version (25K): [in this window] [in a new window]   FIG. 9. Semiquantitative RT-PCR RFLP analysis of -globin gene expression in m 3' ß-YAC transgenic mice. RT-PCRs were performed for wild-type ß-YAC and three m 3' ß-YAC mouse lines. Total mRNAs from 10 day embryonic yolk sac (lanes 1), 12-day fetal liver (lanes 2), 14-day fetal liver (lanes 3), and adult blood (lanes 4) were analyzed using primers for the 5' UTR and exon 1/exon 2 boundary of the -globin gene (see Materials and Methods). The full-length wt-globin product is 164 bp; the m-globin product is 172 bp. The wild-type -globin product is not digested with XhoI, whereas the m-globin product is digested and generates 155-bp and 17-bp fragments. Left panel, wild-type 213-kb ß-YAC transgenic mouse line 4 (wild-type ß-YAC) (40); right panel, m 3' ß-YAC mouse line 2 (Line 2). Results were the same for m 3' ß-YAC transgenic mouse lines 1 and 3.

	DISCUSSION

Top Abstract Introduction Materials and Methods Results Discussion References

The question of the role of gene order in developmental regulation has been previously addressed with various approaches. We demonstrated that when two globin genes from the same developmental stage were arranged tandemly and linked to the LCR, the gene more proximal to the LCR was expressed preferentially throughout development, indicating that proximity to the LCR was a determinant of globin gene expression, perhaps due to the increased frequency of interaction of the proximal gene with the LCR (39). One such arrangement occurs naturally within the human ß-globin locus. The ^G- and ^A-globin coding sequences are nearly identical, but the ^G-globin gene is expressed at higher level than the ^A-globin gene early in fetal erythropoiesis; the ratio inverts as development proceeds (22). When the - and ß-globin genes were linked tandemly to the LCR, -globin was expressed at a higher level than ß-globin during fetal definitive erythropoiesis, and the ratio was reversed during adult definitive erythropoiesis (39). This result occurred regardless of the order of the - and ß-globin genes with respect to the LCR, indicating that the trans-acting environment was the primary determinant of globin gene expression, overcoming other motifs, including proximity to the LCR. Although these experiments suggest that proximity to the LCR plays a role in correct gene expression, the distances between the LCR and linked genes were artificially shortened, leaving open the question as to whether gene order was important.

Another experimental system utilized two cosmid constructs encompassing portions of the human ß-globin locus that were ligated together just prior to producing transgenic mice (10). One set of mouse lines contained a locus in which a ß^m-globin gene was placed upstream of the -globin gene; in another set of lines the -globin gene was replaced with the ß^m-globin gene. In the lines where the ß^m-globin gene replaced the -globin gene, ß^m-globin was expressed throughout development; ß^wt-globin comprised only a small percentage of expression in adult tissues. In addition, -globin gene expression was silenced. In the lines where the ß^m-globin gene was located near the -globin gene, ß^m-globin accounted for 75% of total ß-globin synthesis and ß^wt-globin for 25%. In a third set of experiments, the genes of the human ß-globin locus were inverted with respect to the LCR in ß-YAC transgenic mice (44). In this instance the ß-globin gene was proximal, and it was constitutively expressed. The -globin gene was most distal; it was not expressed at all, and -globin expression was restricted to primitive erythropoiesis. These data suggested that any gene located near the LCR will be strongly expressed throughout ontogeny.

In this study, we used complete, intact locus constructs containing all potential regulatory elements and all the wild-type genes of the locus in their normal spatial configuration. The 213-kb human ß-globin locus YAC contains 187 kb of the locus, and mice generated with this YAC have been shown to display correct tissue-specific and temporal expression patterns (36, 38). A marked ß-globin gene was placed upstream of either the -globin gene or the ^G-globin gene. A marked ^A-globin gene was placed upstream of the -globin gene. A marked -globin gene was placed between the - and ß-globin genes. Several conclusions can be drawn from the expression patterns we observed.

Placement of the marked ß-globin gene upstream to the -globin gene resulted in predominantly ß^m-globin expression during yolk sac erythropoiesis. -Globin expression in embryonic cells was decreased by 50% compared to that for the wild-type ß-YAC -globin gene control, and there was no detectable -globin gene expression. Only ß^m-globin gene expression could be detected during definitive erythropoiesis in fetal liver and adult erythroid cells. Similar results were observed in a previous study in which a ß^m-globin gene replaced the -globin gene (10). The most likely explanation for these results is preferential interaction of the ß^m-globin gene with the LCR due to its proximity to this regulatory element. The absence of downstream ß^wt-globin gene expression can be attributed to competition. It is of interest that although there was -globin gene expression in the embryonic cells, -globin gene expression was absent. This finding suggests that structural (or distance) constraints exist that determine (or influence) the interaction between the LCR and the downstream globin genes. Perhaps the distance between the ß^m- and -globin genes is too short and prohibits the interaction of the LCR with the nearby downstream -globin gene. Similarly, Dillon et al. (10) showed that -globin was expressed during embryonic erythropoiesis when the ß^m-globin gene replaced the -globin gene, but expression was completely suppressed in the fetal liver. Thus, the distance between the -globin gene (or the ß^m-globin gene in this case) and the -globin gene is sufficient to allow interaction of either of these genes with the LCR.

Another conclusion from this study is that it is not so much the placement of a gene within the locus that determines globin gene switching but rather the presence of silencing sequences and their interactions with repressor complexes. Because the ß-globin gene lacks developmentally controlled silencing elements in its promoter, switching is abolished when that gene is located at the beginning of the locus. Conversely, when an ^Am-globin gene was placed at the same location upstream of the -globin gene, the ^Am-globin gene was turned off during definitive erythropoiesis and the switch from - to ß-globin gene expression occurred. These results provide support for the concept that to a major degree, globin gene switching is controlled negatively by the presence of silencing elements in the embryonic and fetal globin gene promoters and the interaction of these elements with the prevailing trans-acting environment. For the -globin genes, the silencing mechanism is leaky and can be overcome by several physiological manipulations, acquired pathological conditions, and mutations in vivo in humans. Also of note here is the fact that the level of ß^m-globin gene expression in the adult stage of development decreased to about 50% of that in the embryonic stage. As will be discussed later, this finding may reflect a change (in chromatin modification?) of the embryonic gene chromatin domain in which the ß^m-globin gene is located.

The placement of the ß^m-globin gene upstream of the ^G-globin gene resulted in phenotypes that could have been predicted on the basis of the hypothesis that gene order and proximity to the LCR determine globin gene expression. Thus, in the embryonic cells there was -, ß^m-, and -globin gene expression, but there was no expression of the distal wild-type ß-globin gene. -Globin expression switched off at the onset of fetal erythropoiesis; -globin gene expression subsequently switched off later. The distal ß^wt-globin gene was expressed at 1/10 the level of the upstream ß^m-globin gene during fetal or adult definitive erythropoiesis. All of these observations were expected based on the hypothesis. Significantly, however, instead of the expected increase of ß-globin gene expression as development proceeded, a consistent decrease of ß^m-globin gene expression was observed, so that total ß-globin gene expression (ß^wt+ß^m) in the adult cells was about 25% of that in the embryonic cells. As mentioned earlier, a decrease of ß^m-globin expression to 50% of that in embryonic cells was noted in adult erythropoiesis when the ß^m-globin gene was located upstream of the -globin gene. How can these unexpected declines in ß^m- and ß^wt-globin gene expression be explained? Changes in the transcriptional environment cannot be the reason, because the environment of the adult erythroid cells is expected to be more conducive to the interaction between the LCR and the ß-globin gene promoter. Perhaps, as suggested, the whole architecture of the ß-globin locus chromatin changes during development, so that an initial embryonic chromatin domain is succeeded by a fetal and finally an adult domain (17). Although this change in the chromatin domain still remains hypothetical, it could provide an explanation for our observations. For example, chromatin modification in the embryonic domain might affect the efficiency of looping of the LCR and decrease its interaction with the ß^m-globin gene placed in that domain. Chromatin modification studies may provide further insight into this question.

The control of -globin gene expression during development has been the subject of considerable discussion. Initial studies by two laboratories suggested that competition between the - and ß-globin genes for interaction with the LCR, coupled with the preferential interaction of the ß-globin gene with the LCR during the adult stage of development, are responsible for the silencing of the -globin gene in adult erythropoiesis (3, 6, 12). When the -globin gene was linked to a µLCR cassette, it was not silenced in the adult stage (13); it was, however, silenced in a cosmid containing the ^G- to ß-globin gene region with these genes in their normal chromosomal location (11). On the other hand, studies with transgenic mice carrying a mini-LCR linked to a -globin gene suggested that silencing could be autonomous, since several (but not all) of these transgenics did not express -globin in adult cells (9). The results of our study in which the ^Am-globin gene was placed upstream of the -globin gene provide support for the possibility of autonomous silencing, since the -globin gene, even that close to the LCR, was turned off in adult erythropoiesis. This experiment also provided information on the relative roles of gene order and transcriptional environment in determining globin gene expression, because only the upstream ^Am-globin gene was expressed in these transgenic mice; the distal -globin genes remained silent. Since the downstream -globin genes remained totally silent during the embryonic/fetal stage of development, it is unlikely that a fetal environment alone is sufficient for -globin gene expression. The results are best explained by assuming that proximity to the LCR is the major determinant of the order of -globin gene expression during development.

As mentioned earlier, some evidence suggests that the ß-globin locus may be divided into developmental stage-specific chromatin subdomains that have a role in determining gene expression (14, 17, 23). Our data show that an open adult chromatin subdomain does not activate ^m-globin gene expression when this gene is placed in the adult chromatin subdomain, between the - and ß-globin genes. More-recent data from our laboratory support this notion, because an ^m-globin gene located between the - and ß-globin genes was not expressed even in the absence of the wild-type -globin gene (unpublished data).

Three major conclusions may be drawn from our data. First, any gene located near the LCR will be strongly expressed throughout ontogeny, unless some gene-specific silencing mechanism exists. Second, the ^A-globin gene is autonomously silenced. Third, both gene order and gene-proximal cis regulatory elements are important for correct developmental expression of the globin genes. Gene order, per se, may not be a direct determinant of gene expression, but ultimately an inherent property of spatial gene order is the maintenance of the necessary distance between the LCR and the globin genes to avoid aberrant gene expression. Thus, gene order constrains distance and LCR proximity. To some extent, alteration of normal LCR proximity supersedes the dominance of the trans-acting environment, particularly when the gene does not have a mechanism of autonomous silencing.