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Institute of Molecular Medicine, National Taiwan University Medical School,1 Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan2
Received 9 September 2004/ Returned for modification 25 October 2004/ Accepted 12 January 2005
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ABSTRACT |
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INTRODUCTION |
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Mcl-1 appears to function at an apical step in many regulatory programs that control cell survival and death. Its synthesis is quickly stimulated after treatment of cells with many cytokines or other signals that lead to cell survival and/or differentiation (2, 6, 10, 11, 14, 25, 33). On the other hand, when cells are deprived of survival factors, stimulated by genotoxic stress (e.g., UV irradiation), or infected by certain viruses, Mcl-1 synthesis is rapidly blocked, and the existing pool of Mcl-1 protein is quickly degraded (6, 8, 24). Overexpression of Mcl-1 delays but does not prevent cells from undergoing apoptosis (6, 26, 38), indicating that Mcl-1 enhances short-term cell survival. In the immune system, Mcl-1 protein levels are rapidly degraded in the extremely short-lived neutrophils that constitutively undergo apoptosis for the resolution of inflammation (1, 22). Moreover, Mcl-1 levels were reported to decrease markedly in peripheral blood B lymphocytes when they undergo spontaneous apoptosis in vitro (19), and the macrophage survival is strictly dependent on the expression of Mcl-1 (18). A recent study by conditional ablation of Mcl-1 in the lymphoid system further indicates that Mcl-1 is required for the development and maintenance of B and T lymphocytes (25). Lastly, Mcl-1 appears to be extremely important for early embryo development, since Mcl-1-null embryos die at the peri-implantation stage (27). Taken together, these studies indicate that proper regulation of Mcl-1 levels is pivotal to the success of many developmental processes, as well as of the various defense responses of the host system.
Cytokine stimulation of Mcl-1 expression is rapid but transient (6). This is mainly because Mcl-1 proteins and mRNA are both extremely labile and are quickly degraded after the initial synthesis step (6, 34). The extremely labile nature of the Mcl-1 protein itself and the fact that the Mcl-1 level is a critical determinant in various cell survival pathways suggest that cellular processes that regulate Mcl-1 stability are as important as those that regulate Mcl-1 synthesis. Although transcriptional stimulation of Mcl-1 synthesis in response to various stimuli has been well documented (6, 30, 31; see also reference 7 for a recent review), the regulation of Mcl-1 stability has been hardly explored.
Translationally controlled tumor protein (TCTP) has been identified in a wide range of eukaryotes, including fungus, yeast, insects, plants, and mammals (3, 20, 28, 36). The extremely high degree of sequence conservation during evolution suggests that TCTP plays an essential role in the development of various organisms (28). Although TCTP was implicated in the human allergic response (20), apoptosis regulation (15), and various other growth-related functions (9, 29, 35), the biological roles of this protein remain elusive.
In the present study, by using a yeast two-hybrid approach, we identified that TCTP was one cellular factor that interacted with Mcl-1, a result consistent with that recently reported by Zhang et al. (37). However, in contrast to their conclusion that Mcl-1 acts as a chaperone of TCTP (fortilin) (37), we found that TCTP bound to Mcl-1 and modulated the stability and antiapoptotic activity of Mcl-1. Furthermore, we demonstrated that TCTP stabilized Mcl-1 via interfering with Mcl-1's degradation by the ubiquitin-dependent proteasome degradation pathway. The role of TCTP in modulating Mcl-1's stability and antiapoptotic activity is discussed.
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MATERIALS AND METHODS |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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C31 and PBTM116hMcl-1C27 are two yeast expression vectors that could direct the synthesis of a fusion protein containing the LexA-binding domain and the mouse or human Mcl-1 protein without the C-terminal transmembrane domain (mMcl-1C31 and hMcl-1C27). These two vectors were used as bait to screen human lymphocyte and mouse embryonic day 7 cDNA libraries according to the manufacturer's instructions (Clontech). All positive clones were processed to recover the plasmid containing the library cDNAs, and the recovered plasmids were reintroduced into the yeast strain L40 transformed with the bait vector to confirm their positivity before their sequence was analyzed by the standard method.
Antibodies.
Mouse Mcl-1-specific polyclonal antibodies were generated in rabbits by using bacterially produced, histidine-tagged mMcl-1C31 protein. TCTP-specific polyclonal antibodies were generated in guinea pigs by using recombinant TCTPN10. For most immunoprecipitation and immunoblotting analyses, partially purified rabbit or guinea pig sera were used. For immunocolocalization experiments, both TCTP and mMcl-1 antibodies were first affinity purified by using specific antigens cross-linked to CNBr-activated Sepharose (Amersham). Other antibodies used in the present study include those specific to hMcl-1, Bcl-2, Bax, ubiquitin, and green fluorescent protein (GFP; all from Santa Cruz Biotechnology); p53 (a gift from Young-Sun Lin, IBMS, Academia Sinica, Taipei, Taiwan); and 
-tubulin (Sigma).
Cells. NIH 3T3 is a mouse fibroblast cell line and was purchased from the American Type Culture Collection. CHOP is a Chinese hamster ovary cell line stably transfected with the polyomavirus large T antigen (9a) and was kindly provided by James W. Dennis (Mt. Sinai Hospital, Toronto, Ontario, Canada). Ba/F3 is a murine interleukin-3 (IL-3)-dependent pro-B-call line, and Ba/F3/hMcl-1 cells are Ba/F3 derivatives stably overexpressing the human Mcl-1 protein as previously described (30). Ba/F3hMcl-1/TCTP cells (clones T-20 and T-23) are Ba/F3/hMcl-1 (clone #23H) derivatives stably overexpressing FLAG-TCTP, whereas the Ba/F3hMcl-1/vector cells (clones V-13 and V-14) are the parental Ba/F3/hMcl-1 cells (clone #23H) stably transfected with an empty vector. Ba/F3/mMcl #Wt-34 and #Wt-38 are two representative clones of Ba/F3 derivatives stably overexpressing the hemagglutinin (HA)-tagged wild-type mMcl-1 protein. Ba/F3/E206R, Ba/F3/K257V and Ba/F3/K260V are all Ba/F3 derivatives stably overexpressing the HA-tagged mMcl-1 mutants with a single amino acid mutation at the position as indicated in their names. All Ba/F3 derivatives were generated by transfection of cells with respective expression vectors by electroporation as previously described (6). To analyze the viability of the Ba/F3 derivatives after deprivation of IL-3, cells were seeded at a density of 105/ml in RPMI 1640 medium supplemented with 10% fetal bovine serum and 10 U of recombinant IL-3/ml. At 2 days after seeding, cells were washed three times with phosphate-buffered saline (PBS) and seeded in low-serum (0.5%) medium without IL-3. Cell viability was determined by the trypan blue exclusion analysis. In some cases, the annexin V staining kit (BioVision) was used to quantify the relative number of cells that had undergone apoptosis.
Expression vectors.
pCDNA3-HA-mMcl-1, pCDNA3-HA-hMcl-1, pCDNA3-BID, pCDNA3-Bad, pCDNA3-Bcl-2, pCDNA3-A1, pCDNA3-HA-AKT, pCDNA3-HA-PU.1, pCDNA-FLAG-Bim, and pCDNA3-p53 are mammalian expression vectors for expressing various proteins as indicated in the construct names. pFLAG-CMV2-TCTP was generated by PCR amplification of the coding region of the mouse TCTP cDNA with two primers (sense, 5'-CCCAAGCTTATCATCTACCGGGACCTCATCAGCCATGACGAGCTGTTCTCCGAC-3';antisense, 5'-GATCGGATCCTTAACATTTCTCCATCTCTAAGCC-3'). The amplified fragments were restricted with HindIII and BamH1 and cloned into the corresponding sites of the pFLAG-CMV2 vector (Kodak). pGEX4T-1-TCTPN10 is a bacterial expression vector which directs the synthesis of a TCTP mutant (without the first 10 amino acids) C-terminally fused to the glutathione S-transferase (GST) protein. This vector was constructed by subcloning the EcoRI fragment of the positive clone obtained from the yeast two-hybrid screen (Fig. 1A, clone 1) into the corresponding sites of the pGEX4T-1 vector (Amersham-Pharmacia). pGEX 4T-1-mMcl-1 was constructed by subcloning the EcoRI fragment of the mouse Mcl-1 cDNA into the corresponding sites of the pGEX 4T-1 vector. The expression vectors encoding the HA-tagged, mMcl-1 mutants (E206R, T240V, K257V, and K260V) were constructed by standard PCR-assisted mutagenesis-coupled cloning methods. The template used for PCR amplification was pCDNA3-HA-mMcl-1. The first PCR was performed with the antisense primer (5'-ATCGGGCCCCTATCTTATTAGATATGCCAGACC-3'), together with one of the following primers (underlined nucleotides differ from the wild-type sequence): E206R (5'-GGCGTGCAGCGCAACCACCGGACGGCCTTCCAGGGCATG-3'), T240V (5'-GTTTTCAAAGATGGCGTAGTAAACTGGGGCAGGATTGTG 3'), K257V (5'-TTCGGTGCCTTTGTGGCCGTACACTTAAAGAGCGTAAAC-3'), and K260V (5'-TTTGTGGCCAAACACTTAGTGAGCGTAAACCAAGAAAGC-3'). The second PCR was performed with the sense primer (5'-TACCGCCAGTCGCTGGAG-3') and the PCR fragment obtained from the first PCR. The resultant Eco47III/ApaI fragment was used to replace the corresponding region of the wild-type mMcl-1 coding sequence within the pCDNA3-HA-mMcl-1 vector. All mutated nucleotides were confirmed by sequencing.
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N10, GST-TCTP, or GST-mMcl-1 fusion proteins were immobilized on glutathione-Sepharose 4B beads (Pharmacia) and then incubated for 2 h at 4°C with in vitro-synthesized, [35S]methionine-labeled proteins as indicated in Fig. 1B. After three extensive washes with the bead binding buffer (50 mM potassium phosphate [pH 7.5], 150 mM KCl, 10 mM MgCl2, 10% [vol/vol] glycerol, 1% [vol/vol] Triton X-100, 0.5 mM phenylmethylsulfonyl fluoride, 5 µg of leupeptin/ml, 10 µg of aprotinin/ml), the bound proteins were resolved on a sodium dodecyl sulfate (SDS)-containing 10% polyacrylamide gel and visualized by fluorography. Coimmunoprecipitation (co-IP) of TCTP and mMcl-1. CHOP cells were transiently transfected with wild-type or mutant mMcl-1 mammalian expression vectors, together with pFLAG-CMV2-TCTP, by the liposome-mediated gene transfer method as previously described (17). At 18 h after transfection, cells were lysed with an ice-cold lysis buffer (10 mM HEPES [pH 7.2], 142.5 mM KCl, 5 mM MgCl2, 1 mM EGTA, 0.2%NP-40, 1 mM phenylmethylsulfonyl fluoride, 5 µg of leupeptin/ml, and 10 µg of aprotinin/ml). Precleared cell lysates were then immunoprecipitated with partially purified antiserum specific to TCTP, mMcl-1, or the corresponding preimmune serum. The immunoprecipitated protein complexes were resolved by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to the polyvinylidene difluoride membrane (Millipore), and the coimmunoprecipitated proteins were analyzed by immunoblotting with anti-HA (Boehringer Mannheim), anti-FLAG M2 (Sigma), or other antibodies as indicated in the figures. After probing with an appropriate horseradish peroxidase-conjugated secondary antibody, specific signals on the membrane were visualized by using an enhanced chemiluminescence system (Amersham) according to the manufacturer's instructions. To analyze the interaction between endogenous TCTP and Mcl-1, the procedure described above was used except that the NIH 3T3 cell lysates were used in the assay.
Indirect immunofluorescence and confocal microscopy. To determine the cellular localization of Mcl-1 and TCTP, NIH 3T3 cells, cultured on coverslips, were fixed in 2% paraformaldehyde in PBS for 15 min. After a brief rinse with PBS at room temperature, cells were blocked and permeabilized in PBS containing 2% goat serum, 0.1% saponin, and 0.02% NaN3 for 15 min. The cells were then incubated at room temperature with affinity-purified rabbit anti-mouse Mcl-1 and guinea pig anti-mouse TCTP antibodies (5 µg/ml each in PBS containing 3% bovine serum albumin). Two hours after incubation, cells were washed with PBS and then incubated with fluorescein-conjugated goat anti-rabbit and Alexa Fluor 568-conjugated goat anti-guinea pig immunoglobulin G (IgG; Molecular Probes) for an additional 1 h at room temperature. After three washes with PBS, stained cells were analyzed with confocal fluorescence microscopy (Zeiss type LSM-5). To show the specificity of the stained signals, cells to be analyzed were processed in an identical way except that the primary antibody used in the staining procedure was preblocked with specific antigen produced in bacterial cells.
Knockdown of TCTP expression with small interfering RNA (siRNA). To knock down TCTP expression in the human cell lines, two TCTP siRNA-expressing vectors (siTCTP#1 and siTCTP#2) were constructed by using the pSUPER-neo+gfp vector (OligoEngine, Inc.). These two constructs were designed according to the manufacturer's protocol in such a way that a duplex siRNA corresponding to the following two regions of the human TCTP gene 5'-AAGGTACCGAAAGCACAGT-3'(siTCTP#1) or 5'-AACCATCACCTGCAGGAAA-3' (siTCTP#2) could be synthesized inside mammalian cells transfected with these vectors (4).
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RESULTS |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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We next carried out the following experiments to validate the specific interaction between Mcl-1 and TCTP. First, the in vitro GST pull-down assay was performed. As shown in Fig. 1B, the [35S]methionine-labeled Mcl-1 protein synthesized by the in vitro transcription-translation system was efficiently and specifically pulled down by the GST-TCTPN10 fusion protein (Fig. 1B, upper panel, lane 2). The observed in vitro binding activity of this fusion protein to Mcl-1 was specifically mediated by the TCTP moiety, since Mcl-1 was not pulled down by the GST protein alone. (Fig. 1B, upper panel, lane 3). The specific in vitro interaction between Mcl-1 and TCTP was also observed with the GST-Mcl-1 fusion protein and [35S]methionine-labeled, in vitro-synthesized TCTPN10 (Fig. 1B, middle panel, lane 3). Interestingly, in this in vitro binding assay, unlike TCTPN10, the full-length TCTP protein did not manifest any significant binding to Mcl-1 (compare the middle and the bottom panels of Fig. 1B, lanes 3).
Next, the in vivo interaction between Mcl-1 and TCTP was examined by a co-IP assay. In this assay, CHOP cells were transiently transfected with expression vectors encoding HA-tagged mMcl-1 (HA-mMcl-1) and FLAG-tagged full-length TCTP (FLAG-TCTP). As shown in Fig. 1C, immunoprecipitation of cell lysates containing HA-mMcl-1 and FLAG-TCTP (see Fig. 1C, lanes 1 and 4, for protein expression) with anti-mMcl-1, but not the preimmune serum, coimmunoprecipitated FLAG-TCTP as revealed by immunoblotting with anti-FLAG antibody (Fig. 1C, compare lanes 2 and 3). In a reciprocal experiment, the TCTP antibody also specifically coimmunoprecipitated HA-mMcl-1 (Fig. 1C, lane 5). Next, we examined whether the interaction between Mcl-1 and TCTP could be observed at endogenous levels of both proteins. For this experiment, co-IP assays with lysates from NIH 3T3 cells were performed. As shown in Fig. 1D, the purified mMcl-1 antibody, but not control rabbit IgG, coimmunoprecipitated endogenous TCTP (Fig. 1D, compare lanes 2 and 3). Also, in a reciprocal co-IP experiment, the mMcl-1 protein was found to be present in the immune complexes pulled down by the TCTP but not by the control guinea pig IgG (Fig. 1D, compare lanes 4 and 5). Lastly, the in vivo interaction between endogenous mMcl-1 and TCTP was further supported by an immunocolocalization experiment with affinity-purified antibodies specific to mMcl-1 and TCTP. As shown in Fig. 1E, the mMcl-1 protein is predominantly localized in the cytoplasm with a punctate staining structure. However, in some cells it is also found localized in the nucleus (Fig. 1Eb). Interestingly, no matter where mMcl-1 resides, the TCTP protein is only partially colocalized with mMcl-1, with some cells more prominent than others (Fig. 1Eb to d and f to h). This partial colocalization result is consistent with the low co-IP efficiency of the endogenous proteins as observed in Fig. 1D (compare Mcl-1 bands, shown in lanes 1 and 6, to those shown in lanes 2 and 4). Taken together, these results demonstrate that, in contrast to the negative interaction as observed in the GST pull-down assay (Fig. 1B, the bottom panel), full-length TCTP and mMcl-1 did interact with each other in vivo (Fig. 1C and D), albeit probably in a cell state-specific manner (see Discussion). Our results also suggest that additional cellular protein(s) might potentially be involved in mediating the TCTP and Mcl-1 interaction inside the cells.
TCTP enhances the protein stability of Mcl-1 but not vice versa. While performing the transient cotransfection experiments for analysis of the in vivo interaction between Mcl-1 and TCTP (Fig. 1C), we noticed that as we increased the amounts of TCTP-encoding plasmids in the transfection experiment, the protein levels of coexpressed HA-Mcl-1 elevated in a dose-dependent manner, despite the fact that equal amounts of HA-Mcl-1-expressing plasmids were used (Fig. 2A, compare lanes 1 to 4). Such TCTP-mediated enhancing effect toward intracellular Mcl-1 levels was specific, since levels of other examined proteins, such as HA-PU.1 (Fig. 2A, lanes 5 to 8), HA-AKT (Fig. 2B), and p53 (Fig. 2C), remained unchanged or marginally affected under identical experimental conditions. Conversely, in a reciprocal experiment with increasing amounts of the HA-mMcl-1 expression vector, we failed to observe any enhanced expression of coexpressed FLAG-TCTP (Fig. 2D). At higher dosages, overexpression of HA-Mcl-1 even inhibited the expression of coexpressed FLAG-TCTP (Fig. 2D, lane 4). Hence, this modulatory effect on protein levels seems to be unidirectional.
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1.5 h). Taken together, these results indicate that TCTP enhances the accumulation of Mcl-1 protein levels through increasing the protein stability of Mcl-1.
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1.5 h). In contrast, under the same experimental conditions, the protein levels of two other TCTP noninteracting Bcl-2 family proteins (Bcl-2 and Bax, see Fig. 6A), and -tubulin remained rather constant both in the control and the TCTP knockdown lines. Taken together, these results indicate that the stability of Mcl-1 is indeed specifically influenced by the cellular levels of TCTP.
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We next compared the protein stability of the K257V mutant to that of the wild-type or other Mcl-1 mutants whose TCTP binding activity was not significantly compromised. We noticed that the turnover rate of all TCTP binding-competent Mcl-1 mutants (only analysis of K260V was shown in Fig. 7) was comparable to that of the wild-type protein (t1/2, 1.5 h), whereas the TCTP binding-defective mutant K257V manifested a much shorter half-life (40 min). Interestingly, only the antiapoptotic activity of the TCTP binding-defective mutant (i.e., K257V) and not that of other TCTP binding-competent mutants was significantly compromised (Fig. 8 and data not shown for the T240V and E206R mutants). Taken together, these results indicate that the stability and the antiapoptotic activity of Mcl-1 strongly correlate with Mcl-1's ability to interact with TCTP.
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DISCUSSION |
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TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
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Given the fact that overexpression of TCTP interferes with Mcl-1's degradation at the ubiquitination step and that the TCTP-binding defective mutant of Mcl-1 (K257V) is much more susceptible to ubiquitination and manifests a compromised antiapoptotic activity, we propose the following two models. First, TCTP may serve as a molecular chaperone of Mcl-1. Without TCTP, the newly synthesized Mcl-1 somehow cannot be properly processed to fold into a mature and functional protein and therefore gets degraded faster. While our work was in progress, we noticed that Zhang et al. reported an interaction between Mcl-1 and TCTP (fortilin) (37). In that study, the authors demonstrated that TCTP was destabilized when the Mcl-1 protein levels were knocked down by RNAi. The authors further suggested that Mcl-1 worked as a chaperone of TCTP and stabilized the latter protein, a result that was seemingly opposite to our observations. Under our experimental conditions, we noticed that TCTP was much more stable (t1/2 > 6 h) than Mcl-1. If Mcl-1 indeed functions to stabilize TCTP as suggested by Zhang et al. (37), it means that the cells would have to constantly stimulate the synthesis of the labile Mcl-1 protein so as to maintain the stability of a much more stable protein, such as TCTP, a cellular condition that does not seem to be logically favorable. Based on this argument and the recent structure study where a chaperone function was proposed for the yeast homolog of TCTP (28), TCTP is more likely to serve as a molecular chaperone of Mcl-1, if a chaperone function is indeed involved in the interaction between Mcl-1 and TCTP.
Alternatively, the functional outcome of Mcl-1 and TCTP interaction can be interpreted by a second model, in which TCTP may be a cofactor of Mcl-1, i.e., Mcl-1 carries out its biological activity only when it forms a functional complex with TCTP. In this model, the highly stable nature of the TCTP protein suggests that its interaction with Mcl-1 is probably dependent on a posttranslational modification of TCTP and possibly of Mcl-1 as well. TCTP was shown to be phosphorylated by the mitotic polo-like kinase Plk (35). Interestingly, overexpression of the Plk phosphorylation site-deficient mutant of TCTP (AA-TCTP) triggered cells to undergo multinucleation or cell death (35). It would be interesting to test whether the AA-TCTP mutant retains the ability to interact with Mcl-1 and modulates Mcl-1's antiapoptotic activity. If the second model is correct, the observed protection of Mcl-1 by TCTP from ubiquitination (Fig. 9A) is probably due to masking of the ubiquitination sites on Mcl-1 when TCTP forms a functional complex with Mcl-1. Under such conditions, this complex formation may also protect TCTP from degradation, a model that would be consistent with Zhang et al.'s finding that the Mcl-1 binding-defective mutant of TCTP is much more susceptible to degradation than the wild-type molecule (37). More experiments are certainly required to test these two models.
In addition to Mcl-1, we also identified a novel interaction between TCTP and another Bcl-2 family member, A1 (Fig. 6A). Although the functional significance of the latter interaction is not clear, we noticed that Mcl-1 and A1 share some molecular characteristics that are unique within the Bcl-2 family. First, they both are immediate-early genes activated by cytokines (6, 12, 16). Second, both mRNAs are extremely unstable (6, 21). It would be interesting to determine whether A1 is also a labile protein and whether TCTP also affects its stability and its antiapoptotic activity.
Growth-related functions have been reported for TCTP. However, the underlying mechanisms still remain largely uncharacterized (9). Recent studies have suggested that the cellular role of TCTP may be partly mediated by its association with factors involved in translation elongation or mitosis/cytokinesis (5, 35). The newly identified ability of TCTP in modulating the activity of the antiapoptotic protein Mcl-1 (and potentially other related factors) further strengthens TCTP's role in cell growth control. We noticed that, consistent with an indispensable role of TCTP in cell growth, some transfected cells (i.e., GFP-positive cells) underwent autophagy-like morphological changes and subsequently died in the course of selecting stable clones that overexpress the TCTP siRNA (data not shown). For stable clones that we were able to establish for subsequent analysis, we noticed that their TCTP levels were not completely knocked down (at least 10 to 20% of the original levels remained). The growth and morphology of these established clones are apparently not too much different from those of their parental cells (data not shown). This observation suggests that whereas the presence of TCTP is critical for optimal proliferation, residual levels of TCTP may be sufficient to carry out most of its biological activities. Under such conditions, the effect of TCTP on the Mcl-1 stability and function in the established TCTP knockdown lines may be underestimated, as residual amounts of TCTP are still present in these cells. An ultimate proof of the modulatory roles of TCTP on the Mcl-1 protein would require the generation of cells whose TCTP expression is completely inhibited, for example, from cells generated by the gene knockout approach.
The Mcl-1 protein was found localized in both the mitochondrial and the nonmitochondrial membrane fractions (34). However, despite the absence of a common nuclear localization sequence, one group did report a predominant nuclear localization of Mcl-1 (15, 37). In our immunofluorescence analysis, we observed that Mcl-1 was predominantly localized in mitochondria when cells were freshly seeded and analyzed on the next day (data not shown). However, if the immunostaining analysis was carried out with cells that had been cultured for a longer time (e.g., at least 2 days after seeding), Mcl-1 was found localized both in nucleus and cytoplasm. In either cellular compartment, we noticed that, whereas Mcl-1 and TCTP were only partially colocalized, such spatial association was more prominent in some cell states than others, suggesting that the interaction of these two proteins is potentially regulated in a cell-state-specific manner. The physiological consequence, as well as the responsible regulatory signaling events, of such cell state-dependent, subcellular translocation of these two proteins is currently not clear and awaits further investigation.
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ACKNOWLEDGMENTS |
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This study was supported in part by an intramural fund from Academia Sinica and by grants NHR1-EX91-9119BN, NSC90-2318-B-001-001-M51, NSC91-3112-B-001-004-M51, and NSC 92-3112-B-001-016 from National Health Research Institutes and the National Science Council of Taiwan to H.-F.Y.-Y.
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FOOTNOTES |
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