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FIG. 3. Effect of lckW97A allele on negative selection. lckW97A mice were crossed with mice expressing the H-Y TCR transgene and thymocytes from lck+ and lckW97A littermates were analyzed by flow cytometry. (A) Numbers of thymocytes from female and male lck+ (filled bars) and lckW97A (open bars) H-Y TCR mice. The graph shows mean total thymocyte numbers and standard deviations based on analysis of 4 pairs of lck+ and lckW97A mice. (B) Expression of CD4 and CD8 on thymocytes from male lck+ and lckW97A H-Y TCR transgenic mice. (C) Effect of anti-CD3 injection on thymocyte numbers in lck+ and lckW97A mice. The graph shows data from lck+ (filled squares) and lckW97A (open circles) animals from three experiments comparing the fraction of thymocytes present 60 h after injection with 5, 15, or 50 µg of anti-CD3 to PBS-injected controls.
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