Files in this Data Supplement:

• Supplemental file 1 - Fig. S1 (Affinities of anti-total Akt and anti-phospho-Akt P-Ser473 antibodies for the three Akt isoforms), S2 (Targeting strategy for generation of Akt2^-/- mice, genotypes of mice from Akt2^+/- matings, and Western blot analysis of Akt2 expression in brain), S3 (Induction of phospho-Akt and downstream targets in testes of wild-type and Akt2^-/- Akt3^-/- mice after in vivo insulin stimulation), and S4 (Activation of Akt in insulin-responsive tissues of wild-type and mutant mice after in vivo insulin stimulation)
  Zipped PowerPoint document, 1.4MB.
• Supplemental file 2 - Tables S1 and S2 (Progeny of Akt2^+/- Akt3^+/- S1 and Akt1^+/-Akt2^+/- Akt3^-/- S2 matings) and S3 (Blood glucose, plasma insulin, and HbA1c levels in mutant mice)
  MS Word document, 49K.