This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sourisseau, T.
Right arrow Articles by Balda, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sourisseau, T.
Right arrow Articles by Balda, M. S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2006, p. 2387-2398, Vol. 26, No. 6
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.6.2387-2398.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of PCNA and Cyclin D1 Expression and Epithelial Morphogenesis by the ZO-1-Regulated Transcription Factor ZONAB/DbpA{dagger}

Tony Sourisseau,1 Anastasios Georgiadis,2 Anna Tsapara,1 Robin R. Ali,2 Richard Pestell,3 Karl Matter,1* and Maria S. Balda1*

Divisions of Cell Biology,1 Molecular Therapy,2 Institute of Ophthalmology, University College London, London, United Kingdom, and Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania3

Received 14 June 2005/ Returned for modification 5 August 2005/ Accepted 3 January 2006


arrow
ABSTRACT
 
The tight junction protein ZO-1 inhibits G1/S-phase transition by cytoplasmic sequestration of a complex formed by CDK4 and the transcription factor ZONAB. Canine ZONAB is the homologue of human DbpA, an E2F target gene that is overexpressed in different carcinomas. Since the ZONAB target genes that are involved in G1/S-phase transition are unknown, we employed the mammary epithelial cell line MCF-10A and cDNA arrays to screen for such genes. We identified genes encoding cell cycle and replication proteins whose expression was altered due to increased ZONAB expression. For proliferative cell nuclear antigen and cyclin D1 genes, we show that increased mRNA levels resulted in increased protein levels and we identified ZONAB-responsive elements in their promoters by using different approaches, including chromatin immunoprecipitation assays. RNA interference and overexpression of ZONAB affected the proliferation of both MCF-10A and MDCK cells as well as the differentiation of MDCK cells into polarized cysts in three-dimensional cultures. These results indicate that ZONAB regulates the transcription of genes that are important for G1/S-phase progression and links tight junctions to the transcriptional control of key cell cycle regulators and epithelial cell differentiation.


arrow
INTRODUCTION
 
Tight junctions (TJs) restrict paracellular diffusion in epithelial and endothelial cells and allow these cells to form selective barriers between compartments of different compositions (12, 65, 67). TJs are composed of transmembrane components that mediate adhesion and form the paracellular diffusion barrier. These proteins interact with a cytoplasmic plaque that consists of adaptor proteins, such as ZO-1, that recruit signaling molecules and interact with the actin cytoskeleton (14, 57). These components recruit different types of signaling proteins that regulate junctional functions as well as cell behavior (46).

TJ-associated proteins have also been linked to the differentiation of epithelial cells. By using MDCK cells, evolutionarily conserved junctional components that regulate cell polarity have been associated with the regulation of epithelial differentiation into cysts in three-dimensional (3-D) cultures (55, 58). Cysts are spheres with an internal lumen formed by a single layer of polarized cells that expose the apical membrane to the internal lumen (74). The capability of epithelial cells to form such cysts is important for the morphogenesis of many epithelial tissues. However, it is not known whether junctional signaling pathways that regulate proliferation and gene expression influence the morphogenesis of epithelial cysts.

ZONAB is a Y-box transcription factor that binds to the SH3 domain of ZO-1 and regulates the expression of the growth factor coreceptor erbB-2 and cell proliferation in a cell density-dependent manner (9-11). The manipulation of ZONAB activity also alters the final cell densities of mature MDCK cell monolayers (9). ZO-1 inhibits ZONAB by cytoplasmic sequestration. Since ZONAB binds CDK4, cytoplasmic sequestration of ZONAB results in reduced nuclear CDK4; hence, the regulation of CDK4 localization is one way by which ZO-1 and ZONAB regulate G1/S-phase transition (9). Because ZONAB is a transcription factor, it is tempting to speculate that ZONAB may also affect cell proliferation by regulating the expression of cell cycle genes. However, such genes are not known.

Y-box transcription factors are multifunctional regulators of gene expression and have been proposed to play a role in promoting proliferation (26, 35). The human Y-box transcription factors DbpA and DbpB/YB-1 were identified since they bind to DNA probes that are derived from the human c-erbB-2 promoter (56). DbpA, the human homologue of canine ZONAB, is up-regulated in cancer tissues (29, 50). In vitro, ZONAB binds to a different subset of inverted CCAAT box sequences than does DbpB/YB-1 (11), suggesting that ZONAB/DbpA binds to Y-box sequences that have different flanking regions than the ones recognized by YB-1/DbpB. However, the physiological relevance of these differences with in vitro DNA interactions is unknown and DbpA/ZONAB-regulated genes that are directly involved in cell proliferation have not been reported.

To identify genes that are regulated by ZONAB and TJs, we developed human mammary epithelial cell lines by using MCF-10A cells that overexpress ZONAB or express the SH3 domain of ZO-1, which inhibits ZONAB function. RNA that was isolated from these cell lines was used to screen for differential expression of proliferation genes by using cDNA arrays. Proliferating cell nuclear antigen (PCNA) and cyclin D1 genes were among those that were up-regulated by ZONAB, and different assays suggest that ZONAB directly regulates the expression of these genes. To analyze the role of the ZONAB-signaling pathway on epithelial differentiation, we tested our different cell lines for the morphogenetic potential to form cysts in collagen gels and found that ZONAB and ZO-1 indeed regulate epithelial differentiation in this model system. Our data thus link the ZO-1/ZONAB-signaling mechanism to the regulation of important cell cycle regulators, cell proliferation and epithelial differentiation.


arrow
MATERIALS AND METHODS
 
Cell lines and proliferation assays. The human nontransformed mammary epithelial cell line MCF-10A was a gift of Adrian Harris (University of Oxford, Institute of Molecular Medicine, United Kingdom). The cell line was subcloned, resulting in the cell line MCF-10A-95, which forms functional TJs (see below). A second MCF-10A cell line from Joan Brugge's laboratory (Department of Cell Biology, Harvard Medical School) was used for comparison in some experiments and was kindly provided by Alan Hall (Laboratory for Molecular Cell Biology, University College London). This second strain was used only when specifically indicated and was named MCF-10A-JB for the purpose of these studies. MCF-10A cells were cultured in Dulbecco's modified Eagle's medium and F-12 supplemented with 5% horse serum, 20 ng/ml epidermal growth factor, 10 µg/ml insulin, hydrocortisone 500 ng/ml, and 100 ng/ml cholera enterotoxin (20, 61). The human retinal pigment epithelial cell line ARPE-19 was a gift of John Greenwood (Institute of Ophthalmology, University College London, United Kingdom) and cultured as described previously (21). For transfection, HA-SH3 and ZONAB expression vectors were used (9, 11). For all functional assays with clonal cell lines, at least three independent clones for each construct were analyzed. For the construction of lentiviral vectors, the ZONAB cDNA was ligated into a pENTRIA vector (Gateway cloning kit; Invitrogen). pENTRIA was recombined with the destination vector giving rise to LNT-ZONAB, which was used to make lentivirus. For the down-regulation of ZO-1, the conserved sequences AAGATAGTTTGGCAGCAAGAG (Z1) and AATGTCCCTGATCTTTCTGAC (Z2) were first targeted with the mU6 plasmid, which contains a mouse U6 promoter, as described previously (9). For down-regulation of ZONAB, the conserved sequences that had been targeted with the mU6 plasmid described previously by Balda et al. (9) were used. The plasmids were first tested in MDCK cells, and both gave efficient down-regulation, whereas control RNA interference (RNAi) vectors did not affect protein expression and cell density. The RNA duplex cassette Z1 was then excised and cloned into the lentiviral pHR'SIN plasmid (with cPPT and the woodchuck posttranscriptional regulatory element and deleted of the 3' long terminal repeat). The new plasmid, pHR'SIN-shZO-1, was used to make lentivirus. The basic pHR'SIN plasmid backbone and the recombinant lentivirus production protocol have been previously described (7, 64). Viral infections were performed at a multiplicity of infection of 50. For tetracycline-regulated RNAi in MDCK cells, a modified mU6 plasmid was used containing a tetracycline operator at the 3' end of the promoter (2). The sequences to target ZO-1 were as described above, and those used for ZONAB were as previously described (1, 9). Cell proliferation and cell density of MCF-10A cells were analyzed as previously described for MDCK cells (9). For synchronization, cells cultured at a low density for 2 days were incubated in medium with 0.2% serum for 48 h.

cDNA arrays. The BD Atlas human cancer 1.2 array (BD Biosciences Clontech) was used according to the manufacturer's instructions. RNA was either isolated from MCF-10A cells that were overexpressing ZONAB or transfected with empty vector. Cells were plated at low confluence and then grown for 4 days, and three independent clones per type of cell line for each analysis were used. Three independent sets of arrays were incorporated into the analysis by using the AtlasImage 2.01 (BD Biosciences Clontech) software for the quantification and comparison of single and combined arrays.

Protein expression and pull-down assays. Total cell extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose for immunoblotting (11). The following antibodies were used: for ZONAB, anti-C-terminus antibody (11); for ZO-1, antipeptide antibody (13); for anti-C-terminus cyclin D1, rabbit polyclonal antibody (Medical & Biological Laboratory); for PCNA, monoclonal antibody PC10 (Cancer Research UK); and for {alpha}-tubulin, monoclonal antibody 1A2 (36). The hemagglutinin-tagged SH3 domain was detected by immunofluorescence using a monoclonal antihemagglutinin (anti-HA) antibody (11, 18). The pull-down assays and fusion proteins used were previously described (8, 11). The cDNAs encoding the SH3 domains of ZO-2 (amino acid residues 586 to 665) and ZO-3 (amino acid residues 464 to 548) were amplified by PCR from an MDCK expression library and cloned into pGEX-4T-3.

Promoter analysis. The electrophoretic mobility shift assays were performed as described previously (11). The reporter gene plasmid of the human PCNA promoter (–560 to +60) in pGL2 was a gift of Michael B. Mathews (New Jersey Medical School) (43). The inverted CCAAT box at nucleotides –90 to –86 was replaced by AAAAA by PCR. The cyclin D1 promoter plasmids were based on the previously published plasmid (4, 69). The inverted CCAAT box at position –527 to –532 was also substituted by AAAAA. For reporter gene assays, ARPE-19 cells were seeded in 48-well dishes at 45,000 cells per well, cultured for 48 h, and then transfected by the calcium phosphate coprecipitation method overnight. The indicated firefly luciferase plasmids and expression vectors were cotransfected with a control plasmid driving renilla luciferase expression (24). After 24 h, luciferase determinations were performed using the dual-luciferase reporter system (Promega, Madison, WI). The relative firefly luciferase activities were calculated by normalizing according to the renilla luciferase activities. The experiments were performed in triplicate, and similar results were obtained from at least three independent experiments. For chromatin immunoprecipitation assays, MCF-10A cells were grown to 50 to 60% confluence before cross-linking with 1% formaldehyde (70). Immunoprecipitations were performed by using anti-ZONAB antibody covalently coupled to Sepharose beads (11). A total of 5% bovine serum albumin (BSA) was used to reduce nonspecific protein binding to the Sepharose. Coprecipitated promoter sequences were then analyzed after extraction of the DNA by PCR using the following primers: erbB-2, 5'-GGG CAG AGT CAC CAG CCT CTG-3' and 5'-CCC ATG GCT CCG GCT GGA CC-3'; PCNA, 5'-AAG CTT TAT ACA ATG AAC GAT TGA GTG-3' and 5'-CGA CGA CCG GCT GAG ACC TAG-3'; cyclinD1, 5'-GTC GCA TCT TGC TGT GAG CAC C-3' and 5'-CTC TGC CTG GGA CAA GAC CAC C-3'; and ß-actin, 5'-AAA CTC TCC CTC CTC CTC TTC C-3' and 5'-TCG AGC CAT AAA AGG CAA CTT-3'.

3-D cultures. MDCK cell cysts were grown in 3-D collagen gels with modifications of a previously described method (47, 54, 71). Briefly, MDCK cells were trypsinized, diluted 1:3 with serum-containing medium to inactivate trypsin, spun, and then resuspended to a single cell suspension and counted. Cells (20,000 in 5 µl) were mixed with 100 µl collagen-Matrigel master mix that was prepared by neutralizing 60 µl of ice-cold solution containing 1 mg/ml calf skin type I collagen (Sigma; C8919) with 10 µl 10x Dulbecco's modified Eagle's medium, 2 µl HEPES 1 M (pH 7.4), and 2 to 3 µl of 2 M NaOH that was then mixed with 10 µl of 100% fetal bovine serum and 16 µl of Matrigel (growth factor reduced; BD Biosciences). The cell-collagen-Matrigel mix was plated in a well of a 48-well dish containing a coverslip that had been covered with 100 µl of the collagen-Matrigel master mix for 1 h at 37°C. The plated mixture was then allowed to form a solidified gel at 37°C prior to the addition of culture medium. The medium was replaced every 2 days, and the cysts were allowed to develop over 5 to 6 days. For fluorescence labeling, cells were fixed with 3% paraformaldehyde for 20 min at room temperature and were then washed twice with phosphate-buffered saline (PBS). Then, the cells were blocked and permeabilized for 30 min at room temperature with 2% BSA, 1% Triton X-100, and 25 mM Tris in PBS, followed by incubation overnight at 4°C with a goat anti-beta-catenin antibody. After three washes with 2% BSA, 1% Triton, 25 mM Tris in PBS, the samples were incubated with a Cy3-conjugated anti-goat antibody, fluorescein isothiocyanate (FITC)-phalloidin and Hoechst 33528. After three washes with PBS, cells were mounted with Mowiol. For quantification of the different structures, low-magnification pictures from the actin stainings were taken, which allowed for differentiation between the different types of structures as described in Results. At least 10 pictures of each cell line and condition were quantified from two experiments.


arrow
RESULTS
 
Proliferation of MCF-10A cells is regulated by ZONAB. As a first step to identifying genes that are regulated by ZONAB, we developed MCF-10A cell lines that either overexpress ZONAB or express the HA-tagged SH3 domain of ZO-1, which functions as a ZONAB inhibitor (11). Several clones with high levels of expression of the transfected proteins were selected by immunoblotting for ZONAB and by immunofluorescence for the SH3 domain (Fig. 1A and B). To test the specificity of ZONAB for the SH3 domain of ZO-1, we performed pull-down assays with recombinant proteins, including a range of different SH3 domains. Figure 1C shows that ZONAB does not bind to the SH3 domains of Abl, Src, Crk, and Grb2 or the homologue proteins ZO-2 and ZO-3. These results suggest that the binding of ZONAB to the SH3 domain of ZO-1 is a specific interaction.


Figure 1
View larger version (45K):
[in this window]
[in a new window]
  		FIG. 1. ZONAB regulates cell proliferation and density of MCF-10A cells. (A) Equal amount of proteins of independent clones of MCF-10A cells transfected with empty vector or a plasmid that was driving ZONAB-B expression were analyzed by immunoblotting by using anti-ZONAB antibodies. Nontransfected cells express comparatively small amounts of ZONAB that cannot be seen on short exposures (see panel F for a comparison). (B) Expression of the HA-tagged SH3 domain of ZO-1 in MCF-10A cells was analyzed by immunofluorescence. Only clone S7 is shown, which is representative of the three clones used. (C) Pull-down assays using SH3 domains of the indicated proteins as glutathione S-transferase (GST) fusions were bound to glutathione-agarose and incubated with equal amounts of His6-ZONAB. The pull downs were analyzed by immunoblotting (IB) by using either anti-His tag or anti-GST antibodies. (D) MCF-10A cells transfected with the empty vector, overexpressing ZONAB or expressing the HA-tagged SH3 domain of ZO-1 were grown for 4 days (low confluence) or 14 days (high confluence) in growth factor-containing medium. Where specified, cells had been synchronized and were then stimulated to enter S phase for 20 h. S-phase cells were detected by BrdU incorporation. Shown are means ± 1 standard deviation (error bars) of three clones for each transfection that were analyzed twice. *, samples with P values of <0.05 in t tests. (E) Cell density of clonal and lentivirus-transduced MCF-10A-95 cells were analyzed after culturing to full confluence for 14 days. Cells were then trypsinized and counted. Error bars indicate standard deviations. *, samples with P values of <0.05 in t tests. (F) The lentivirally transduced MCF-10A cells were analyzed for expression of ZONAB and ZO-1. Equal amounts of protein were loaded and confirmed by immunoblotting with antitubulin ({alpha}-tubulin) antibodies.

We next tested whether ZONAB also regulates proliferation and G1/S-phase progression for MCF-10A cells as it does for MDCK cells. Two days after plating, cells were either left in growth factor containing medium or synchronized in G1 phase by culturing in low serum. After 2 days, fresh medium with growth factors was added for 20 h and the fraction of cells in S phase was measured by determining the number of cells that were incorporating bromodeoxyuridine (BrdU). Figure 1D shows that overexpression of ZONAB increased BrdU incorporation twofold, whereas the expression of the SH3 domain of ZO-1 reduced proliferation. The results were similar for continuously proliferating and synchronized cells. Moreover, stimulation of G1/S-phase progression was also observed if the cells were cultured at a high density, suggesting that ZONAB overexpression reduced cell density-dependent inhibition of the proliferation of MCF-10A cells. Consequently, the overexpression of ZONAB also increased the cell numbers in MCF-10A monolayers (Fig. 1E).

To exclude the possibility that these phenotypic changes were caused by the selection process, we developed lentiviral vectors to overexpress ZONAB-B or to down-regulate ZO-1 by RNA interference. These viruses were then used to infect the MCF-10A-95 cell line. As expected, the resulting cell lines expressed either high levels of ZONAB or reduced levels of ZO-1 (Fig. 1F) and grew to larger cell numbers that were comparable to those of the above-described stable clones of ZONAB-overexpressing lines (Fig. 1E). The depletion of ZO-1 also increased the final cell density in MDCK cells (1). As MCF-10A cells that were infected with a virus inducing RNA interference of ZONAB stopped proliferating, we could not perform a quantitative analysis of the effect of ZONAB depletion.

To test whether the mammary epithelial MCF-10A cells are a suitable human epithelial cell line for studying the signaling pathways of ZO-1 and ZONAB, two proteins that are recruited to TJs in MDCK cells, we analyzed whether wild-type MCF-10A cells form TJs and express the two proteins. Immunofluorescence revealed that the analyzed MCF-10A cell line (MCF-10A-95) expresses the TJ membrane proteins claudin-4 and occludin and recruits ZO-1 and ZONAB to the junctional complex (see Fig. S1A in the supplemental material). In contrast to a previously described and widely used MCF-10A strain that does not form TJs (MCF-10A-JB) (19, 20, 22), TJ proteins exhibited an apparently continuous junctional distribution in the MCF-10A-95 cells used here, suggesting the presence of functional TJs. Indeed, values for transepithelial electrical resistance were comparable to those of MDCK-II cells (see Fig. S1B in the supplemental material). The depletion of ZO-1 and overexpression of ZONAB did not have strong effects on TJ formation under standard culture conditions; however, the depletion of ZO-1 led to a slight reduction in transepithelial electrical resistance (see Fig. S1C and D in the supplemental material), which is in agreement with previously published results obtained with other cell lines (1, 9, 66). Nevertheless, the data presented here indicate that ZONAB regulates the proliferation of mammary epithelial cells as it does that of MDCK cells.

ZONAB induces up-regulation of PCNA and cyclin D1. We next generated probes to hybridize cDNA arrays of human cancer genes (BD Atlas Clontech PT3547-3E arrays) from the stable MCF-10A cell lines that were transfected with either control vector or overexpressing ZONAB. Three different screenings were performed, which were always pooling RNA isolated from three independent clones for each type of probe.

Table 1 shows a summary of the quantification of these hybridizations. Shown are only cDNAs that gave sufficiently clear signals for analysis; these are arranged in groups corresponding to oncogenes, tumor suppressors, and proteins involved in cell cycle, DNA replication, DNA damage, energy metabolism, chromatin remodeling, and ribosomal function. In agreement with our previous observations in MDCK cells (11), ZONAB overexpression reduced mRNA levels of erbB-2 by two-thirds, indicating that it suppresses the expression of this gene as it does that in MDCK cells. Interestingly, erbB-3 mRNA was up-regulated. Future studies will address the relevance of these observations for signaling by erbB receptors. ZONAB overexpression also increased the expression levels of other transcription factors, such as c-jun and c-myc. Importantly, several cell cycle regulators and genes coding for proteins involved in DNA synthesis and repair were up-regulated, suggesting that ZONAB might also stimulate cell cycle progression by regulating the expression of cell cycle regulators. Each group contains genes that were not affected by ZONAB expression, suggesting that the observed differences are specific.


View this table:
[in this window]
[in a new window]
  		TABLE 1. cDNA array analysis of cell cycle and cancer genes in MCF-10A cellsa

Because ZONAB overexpression significantly increased G1/S-phase progression of MCF-10A cells, we focused on proliferating cell nuclear antigen, a protein that participates in DNA replication and is required during different steps of the cell cycle, and cyclin D1, a key regulator of G1/S-phase transition. The array analysis indicated that both genes were up-regulated at the mRNA level in ZONAB-overexpressing cells (Table 1). Both promoters contain potential Y-box factor binding sites (48, 69).

ZONAB induces PCNA protein expression. We next tested whether increased cell proliferation and mRNA levels of PCNA in ZONAB-overexpressing cells correlates with changes in protein expression. To rule out possible clonal variability, we also used cell lines generated with the lentiviral vector by using one of the control clones as a starting cell line. The cells were cultured at a low density for 4 days to assay expression during continuous proliferation or were synchronized in 0.2% serum and stimulated with growth factors for 20 h to trigger G1/S-phase progression.

Figure 2A shows that PCNA protein expression was higher in ZONAB-overexpressing cells under both conditions (continuous growth and synchronized G1/S-phase progression). Furthermore, the inhibition of ZONAB-function by the expression of HA-SH3, which inhibited proliferation (Fig. 1D), reduced the expression of PCNA protein (Fig. 2A). The stimulation of PCNA expression by ZONAB is not restricted to MCF-10A cells but was also observed in ARPE-19, a human retinal pigment epithelial cell line (Fig. 2B). These results show that the level of ZONAB activity affects the amount of PCNA protein that is expressed in MCF-10A cells.


Figure 2
View larger version (35K):
[in this window]
[in a new window]
  		FIG. 2. Regulation of PCNA expression by ZONAB. (A) MCF-10A cells transduced with lentivirus (lenti) expressing ZONAB-B or stably expressing the HA-tagged SH3 domain of ZO-1 were either grown for four days in growth factor-containing medium or synchronized and then analyzed without or with stimulation with growth factors for 20 h. Equal amounts of proteins were analyzed for PCNA expression by immunoblotting using anti-PCNA monoclonal antibody. (B) Equal amounts of proteins of transduced ARPE-19 cells were analyzed for PCNA expression as described for panel A. lenti, lentivirus. (C) Double-stranded oligonucleotides from the PCNA, erbB-2, and NF-{kappa}B promoters were labeled with 32P, incubated with GST or GST-ZONAB as indicated and analyzed by gel electrophoresis. For competition experiments, unlabeled oligonucleotides (none [0] or 500 pmols of PCNA, erbB2, or NF-{kappa}B) were used to quench binding of the PCNA or erbB-2 probe (5 pmols) as indicated. (D) Reporter gene assays were performed by cotransfecting ARPE-19 cells with a wild-type PCNA promoter reporter plasmid encoding firefly luciferase, a control renilla luciferase plasmid, and different concentrations of pCB6-ZONAB-B expression plasmid (DNA concentrations were equalized with empty expression plasmid). Expression of the luciferases was analyzed, and the values obtained for renilla luciferase were used for normalization. Shown are means ± 1 SD (error bars) of a typical experiment performed in triplicate.

ZONAB binds to the PCNA promoter in vivo. The PCNA promoter contains an inverted CCAAT box at nucleotides –90 to –86. Oligonucleotides containing this region bind to ZONAB in an electrophoretic mobility shift assay and are able to reduce the binding of an oligonucleotide containing the ZONAB-binding site of the erbB2 promoter (Fig. 2C) (11). To test whether ZONAB can regulate the PCNA promoter in vivo, we performed reporter gene assays by using the human promoter containing nucleotides –560 to +60 driving firefly luciferase expression (a kind gift from M. Mathews). We transfected this plasmid with a control reporter plasmid driving the expression of renilla luciferase and different concentrations of pCB6 expression vectors encoding ZONAB. The expression of renilla luciferase was then used to normalize the values obtained for firefly luciferase. Since we were not able to obtain sufficient transfection efficiencies in MCF-10A cells for this reporter promoter analysis, we used another human epithelial cell line derived from an ectodermal tissue, the human retinal pigment epithelial cell line ARPE-19, in which ZONAB also induces up-regulation of PCNA expression (Fig. 2B).

Figure 2D shows that cotransfection of expression vectors containing the ZONAB cDNA increased the activity of the PCNA promoter in a concentration-dependent manner. These results indicate that the manipulation of ZONAB affects the transcriptional activity of the human PCNA promoter in the same way, as it changes the levels of PCNA mRNA and protein expression.

To test whether the effect of ZONAB is due to the association with the promoter in the context of its local chromatin structure, we performed chromatin immunoprecipitation assays by using antibodies specific for ZONAB. Partially cross-linked, sheared chromatin was prepared from MCF-10A cells that were cultured for 4 days in growth factor-containing medium. After immunoprecipitation, PCR assays were performed to determine the presence of specific regions of chromatin that were immunoprecipitated with beads conjugated with antibodies against ZONAB or with negative beads.

Figure 3A shows that anti-ZONAB antibodies precipitated chromatin containing the region of the PCNA promoter that includes the inverted CCAAT box. Similarly, the region of the erbB-2 promoter, a previously identified ZONAB target gene that contains the ZONAB binding site, was also amplified. Precipitates with negative beads did not result in positive PCR. Similarly, PCR with primers that were specific for the ß-actin gene did not result in detectable amounts of PCR product from ZONAB immunoprecipitations. These findings indicate that ZONAB associates with the endogenous PCNA promoter in vivo.


Figure 3
View larger version (22K):
[in this window]
[in a new window]
  		FIG. 3. ZONAB binds to the PCNA promoter in vivo. (A) MCF-10A cells were cross-linked, extracted, and immunoprecipitated with anti-ZONAB antibodies or negative control beads. The precipitated ZONAB-chromatin complexes were then analyzed by PCR for the presence of PCNA and erbB-2 promoter sequences. ß-actin primers were used as a negative control. Input label PCRs in which total nuclear cell extracts were used as template. Plasmids containing the respective promoter sequences were used as positive controls. (B) Reporter gene assays were performed by cotransfecting ARPE-19 cells with a control renilla luciferase plasmid and pCB6-ZONAB-B, pCB6-HA-SH3, or empty pCB6, together with a firefly luciferase reporter plasmid containing the wild-type PCNA promoter or a promoter in which the inverted CCAAT box sequence had been substituted. Shown are means ± 1 standard deviation (error bars) of a typical experiment performed in triplicate. *, samples with P values of <0.05 in t tests.

To determine whether the inverted CCAAT box is indeed part of the ZONAB binding site, we tested whether it is required for the regulation of the promoter by ZONAB. We constructed a new reporter plasmid, in which the inverted CCAAT box was substituted by AAAAA. We then repeated the reporter gene assays under the same conditions as described above. With the wild-type PCNA promoter, luciferase expression was stimulated by cotransfection of ZONAB and inhibited by the SH3 domain (Fig. 3B). In contrast, the mutated PCNA promoter, in which the inverted CCAAT box had been inactivated, did not respond to coexpression of ZONAB or the SH3 domain.

These experiments identify the PCNA gene as a target of ZONAB and indicate that the inverted CCAAT box of the PCNA promoter is essential for the transcriptional regulation by ZONAB.

ZONAB increases cyclin D1 expression. We next focused on the key cell cycle regulator cyclin D1, which was also found to be up-regulated upon ZONAB overexpression in the cDNA array analysis. Figure 4A shows that MCF-10A cells that were overexpressing ZONAB expressed more cyclin D1 if grown continuously for 4 days or if stimulated with growth factors for 20 h after synchronization in low-serum-containing medium. In agreement, MCF-10A cells stably expressing the HA-tagged SH3 domain of ZO-1 showed decreased cyclin D1 protein expression. Thus, ZONAB activity affects the protein level of cyclin D1, suggesting that this important cell cycle regulator might be another gene transcriptionally regulated by ZONAB.


Figure 4
View larger version (28K):
[in this window]
[in a new window]
  		FIG. 4. Regulation of cyclin D1 expression by ZONAB. (A) Control cells, ZONAB-B-overexpressing cells, and HA-SH3-expressing MCF-10A cells were grown for 4 days in growth factor-containing medium or synchronized and then stimulated with growth factors for the indicated times. Equal amounts of protein were then analyzed by immunoblotting using an anti-cyclin D1 antibody. (B) Immunoprecipitated ZONAB-chromatin complexes were tested for the presence of cyclin D1 promoter sequences by using PCR. The used input and precipitates were derived from the same experiment as the one described in the legend for Fig. 3A. (C) Transcriptional regulation of the cyclin D1 promoter by ZONAB requires an inverted CCAAT box. Reporter gene assays were performed by cotransfection of ARPE-19 cells with a control renilla luciferase plasmid and either pCB6-ZONAB-B, pCB6-HA-SH3 or empty pCB6 plasmid and a firefly luciferase reporter plasmid containing the wild-type cyclin D1 promoter or a promoter in which the inverted CCAAT box had been substituted. Shown are means ± 1 standard deviation (error bars) of a typical experiment performed in triplicate. *, samples with P values of <0.05 in t tests. (D) Double-stranded oligonucleotides from the cyclin D1, erbB-2, and NF-{kappa}B promoters labeled with 32P were incubated with GST or GST-ZONAB as indicated and analyzed by gel electrophoresis. For competition experiments, unlabeled oligonucleotides (500 pmols for cyclin D1, erbB2, or NF-{kappa}B) were used to quench binding of the cyclin D1 or erbB-2 probe (5 pmol) as indicated.

To test whether the regulation of ZONAB expression can also be observed in an epithelial cell line derived from a different tissue and to determine whether normal ZONAB expression is required for cyclin D1 expression, we developed MDCK cells allowing the tetracycline-regulated depletion of ZONAB and ZO-1. Figure 5A shows that, when such conditional RNAi cell lines were cultured in the presence of tetracycline, the depletion of ZO-1 and ZONAB was observed. As previously reported for the constitutive depletion of ZONAB (9), tetracycline-induced RNA interference also inhibited the proliferation of MDCK cells (Fig. 5B). Both ZO-1- and ZONAB-depleted cell lines formed monolayers with functional junctions under normal culture conditions if given sufficient time (not shown). This is in agreement with previous studies in mouse mammary epithelial cells and MDCK cells (11, 66).


Figure 5
View larger version (55K):
[in this window]
[in a new window]
  		FIG. 5. ZONAB increases cyclin D1 expression in MDCK cells. (A) MDCK cells conditionally expressing either control, ZO-1 or ZONAB-directed RNA duplexes were plated and grown in the absence or presence of tetracycline (TC) for 3 or 2 days. Total cell extracts were then analyzed by immunoblotting with antibodies against ZO-1, ZONAB or tubulin ({alpha}-tubulin). (B) Equal numbers of conditional control or ZONAB RNAi MDCK cells were plated and then grown with or without tetracycline (TC) for 3 days, and phase-contrast pictures were taken to monitor proliferation. (C) MDCK wild-type cells, two independent clones of ZONAB RNAi cell lines, a ZO-1 RNAi line, and a cell line overexpressing ZONAB-A were plated at low confluence with tetracycline; after 36 h, they were synchronized by serum starvation. S-phase entry was then stimulated by adding 5% serum for 12 or 24 h. Total cell extracts were analyzed by immunoblotting with antibodies against ZO-1, ZO-2, ZONAB, cyclin D1 or tubulin ({alpha}-tubulin).

We next tested whether ZONAB affects the expression of cyclin D1 in MDCK cells that were synchronized in low-serum-containing medium as previously described (9). S-phase entry was then stimulated by serum addition for 12 and 24 h. Figure 5C shows that cyclin D1 expression was still low after 12 h in the wild type as well as in two clones of ZONAB-depleted cells. In contrast, ZO-1 depletion and ZONAB overexpression resulted in increased cyclin D1 expression. After 24 h, cyclin D1 expression increased in wild-type cells but remained low in ZONAB-depleted cells, suggesting that the depletion of ZONAB inhibits cyclin D1 expression. Without tetracycline, all RNAi cell lines expressed similar levels of cyclin D1 (not shown). These data show that ZONAB depletion inhibits the expression of cyclin D1 and that increasing ZONAB activity by overexpression of the transcription factor or depletion of ZO-1 accelerates up-regulation of cyclin D1 during G1/S-phase transition.

Regulation of cyclin D1 promoter by ZONAB depends on the inverted CCAAT sequence. Transcriptional regulation of cyclin D1 is driven by several cis-acting elements binding AP-1, Ets, E2F, NF-{kappa}B, Sp1, CRE, and TCF/LEF (3, 4, 30, 33, 40, 49, 60, 63, 68). It was therefore important to determine whether ZONAB interacted with the cyclin D1 promoter or whether the effect on expression was indirect. The promoter of cyclin D1 contains an inverted CCAAT sequence at positions –527 to –532. We therefore used the precipitates from the chromatin immunoprecipitation assays in Fig. 3A to detect coprecipitation of the cyclin D1 promoter by using primers flanking the inverted CCAAT box. Figure 4B shows that a cyclin D1 promoter product was amplified from ZONAB immunoprecipitations but not from negative beads. Thus, ZONAB associates with the cyclin D1 promoter in the context of its local chromatin structure in vivo.

We next performed luciferase reporter assays to determine the functional importance of the inverted CCAAT box for the transcriptional regulation of the cyclin D1 promoter by ZONAB. ARPE-19 cells were cotransfected with a control renilla luciferase plasmid, a reporter firefly luciferase plasmid containing the wild type, or a mutant cyclin D1 promoter in which the CCAAT box had been substituted as well as pCB6-ZONAB-B, pCB6-HA-SH3, or empty pCB6. Figure 4C shows that twofold increases in the transcription driven by the cyclin D1 promoter were observed when ZONAB was cotransfected, whereas a small inhibition was observed when HA-SH3 was coexpressed. In contrast, stimulation by ZONAB and inhibition by HA-SH3 were not observed when the mutant promoter was analyzed. Moreover, ZONAB was found to interact specifically with double-stranded oligonucleotides containing the inverted CCAAT box-containing sequence of the cyclin D1 promoter in gel shift assays (Fig. 4D). Thus, the inverted CCAAT box is required for the regulation of the cyclin D1 promoter by ZONAB in vivo and interacts with the Y-box factor in vitro.

ZONAB regulates differentiation of MDCK cells in 3-D cultures. We next used 3-D cultures to determine whether the manipulation of ZONAB influences the differentiation potential of epithelial cells. As we were unable to find conditions permitting the formation of cysts by MCF-10A-95 cells and the down-regulation of ZONAB-arrested MCF-10A proliferation, we used MDCK cells. The cultures were started by plating the same number of cells of the different MDCK lines in a mixture of collagen I and Matrigel. Conditional RNAi cell lines had been pretreated with or without tetracycline for 2 days. After 5 days of culture, the cells were fixed in 3% paraformaldehyde and were then fluorescently labeled using antibodies against beta-catenin, FITC-phalloidin to stain actin filaments, and Hoechst 33528 to visualize nuclei. Morphology of the formed structures was then assessed by fluorescence microscopy. Epifluorescence was used for quantification, and confocal microscopy was used for a more detailed morphological analysis of the obtained structures.

Figure 6A shows representative areas of the 3-D structures that were obtained with the different cell lines, and Fig. 6B shows a quantitative analysis for which the structures were classified as cell aggregates without a lumen, cysts with a single lumen, structures with multiple lumen, or other structures. The last class contains structures that did not represent clear spheres, cells that formed monolayer-like structures, and cells that formed aggregates with no clear organization.


Figure 6
View larger version (30K):
[in this window]
[in a new window]
  		FIG. 6. ZONAB regulates cyst formation of MDCK cells grown in 3-D cultures. Control, ZONAB, and ZO-1 RNAi lines as well as ZONAB-A and B overexpressing MDCK lines were plated in the absence or presence of tetracycline (TC) in collagen-Matrigel cultures for 5 days. Cells were then fixed with 3% paraformaldehyde and processed for epifluorescence by using a Cy3-conjugated secondary antibody to detect beta-catenin (red), FITC-phalloidin (green) to detect actin, and Hoechst 33528 (white) to stain DNA. Wild-type and control RNAi cells formed normal cysts without or with tetracycline as the shown control cultures. Shown are overview images of larger fields (a1, a3, b1, b3, c1, d1, and d2) and larger magnifications of characteristic structures (a2, a4, b2, b4, and c2). (B) In two independent experiments, as in the one shown in panel A, pictures from 10 different areas were taken and quantified by counting the number and types of structures. The formed structures were divided into the following classes: spherical structures with no lumen as in a4, a single lumen as in a2, multiple lumens or structures that were not spherical and had a generally disorganized appearance as the ones shown in panels b4, d1, and d2.

This analysis revealed that about half of the control cultures and noninduced RNAi cell lines formed classical cysts with a single lumen. The multiple lumen structures were usually cysts with two apparently separate lumens but that appeared otherwise well organized. Together, cysts with one or two lumens accounted for more than two-thirds of all structures in such control cultures. In contrast, when ZONAB was down-regulated, almost no normal cysts developed and only small aggregates of cells were observed that often did not have any clearly polarized organization (Fig. 6A, panels a3 and a4). If ZO-1 was down-regulated, structures developed to a normal size but only a few developed a normal morphology with a single lumen (less than 10%); many structures were spheres without lumen or were disorganized (Fig. 6, panels b3 and b4). Similar to ZO-1 depletion, the overexpression of either one of the two ZONAB isoforms (Fig. 6A, panels d1 and d2) led to a large number of disorganized structures, suggesting that overexpression of the transcription factor has a consequence similar to that of the depletion of ZO-1, which functions as a ZONAB inhibitor. As such striking morphological defects in cells with reduced ZO-1 expression or increased ZONAB expression were observed in only 3-D cultures, these results suggest that the differentiation function of TJs depends on the extracellular environment.


arrow
DISCUSSION
 
We present evidence that ZONAB regulates G1/S-phase progression in mammary epithelial cells and up-regulates the expression of PCNA, a crucial component of the DNA replication machinery, and cyclin D1, a key regulator of G1/S-phase transition. ZONAB's effect on gene expression and cell proliferation correlates with a reduced differentiation potential of MDCK cells grown in 3-D cultures. Our data thus indicate that the ZO-1/ZONAB pathway regulates the G1/S-phase transition of epithelial cells by two mechanisms (transcriptional regulation of cell cycle and DNA replication factors and, as we have previously demonstrated, modulation of the nuclear accumulation of CDK4, a kinase that regulates G1/S-phase transition) and suggests that this junctional signaling pathway is important for the differentiation of epithelial cells.

Overexpression of ZONAB increases cell density but not proliferation in MDCK cells, whereas it increases cell density and proliferation in MCF-10A cells, suggesting that ZONAB function is cell context dependent. There are many differences between MDCK and MCF-10A cells that will guide future experiments in attempting to identify how ZONAB function is regulated in different cellular contexts. For example, genetic abnormalities, such as the deletion of the locus containing p16INK4a and p14ARF, might affect the function of the CDK4/ZONAB complex in MCF-10A cells (20, 62). It is also possible that the difference between the two cell lines is due to differences in the endogenous expression levels of ZONAB and ZO-1 or to the degree of junctional organization. Nevertheless, the MCF-10A cells (MCF-10A-95) that are analyzed here express the ZONAB-binding partner ZO-1 as well as other TJ proteins, such as occludin and claudin-4, and develop a functional paracellular barrier.

ZO-1 knockout by homologous recombination in the mouse mammary epithelial cell line Eph4 has recently been shown to have a mild phenotype and no change in cell number (66). In contrast, we have observed increased cell densities in both MCF10-A and MDCK cells if ZO-1 was down-regulated, suggesting that proliferation arrest in EpH4 cells is differently regulated. Interestingly, oncogenic Ha-Ras induces increased cell proliferation in EpH4 mammary epithelial cells only if they were cultivated as organotypic structures in three-dimensional collagen-Matrigel matrices but not in standard two-dimensional cultures as in the one used by Umeda et al. (66), suggesting that proliferation is regulated by the culture condition (32). TJs appear to be closely linked to Ras effector pathways: the transmembrane protein occludin can suppress oncogenic Raf-1 signaling, and RalA binds to ZONAB (24, 41). It is thus striking that modulation of the ZO-1/ZONAB pathway only significantly affects the differentiation of MDCK cells in 3-D cultures and not when grown as standard 2-D cultures. It will thus be necessary to test whether ZO-1 knockout in Eph4 cells will have a stronger phenotype when cultivated in three-dimensional collagen-Matrigel matrices.

PCNA is an essential eukaryotic DNA replication factor that also plays a role in DNA repair (45, 53). The cDNA array analysis suggested that ZONAB overexpression results in the up-regulation of several components that either participate in replication (e.g., RFC40 and replication licensing factors) or in chromatin remodeling (histone H4 and HMG-I) or are part of the cellular machinery for DNA repair (e.g., Rad23A and UBE2A). It thus seems that ZONAB promotes S-phase progression by up-regulating the expression of proteins that are required for DNA replication. Additionally, it is possible that ZONAB might participate in stress-related signaling responses by up-regulating DNA repair genes.

The PCNA promoter can be transcriptionally regulated by various oncoproteins and transcription factors, such as adenovirus oncoprotein E1A, cAMP response element-binding protein (CREB), RFX1 transcription factors, the coactivator CREB-binding protein (CBP), tumor suppressor p107, p53, and E2F (15, 34, 37-39, 42, 43, 51, 59). The ZONAB binding site identified here is between the p53 (–237), E2F (–84), and ATF-1 (–52) sites. As it is currently unknown how ZONAB-binding to a promoter can cause up- or down-regulation of the transcriptional activity, it will be interesting to determine whether these binding sites and the proteins that bind to them affect each other.

As ZONAB regulates G1/S-phase progression, the identification of cyclin D1 as a ZONAB target gene is of particular importance. Cyclin D1 plays a central role in cell cycle control, and its expression is regulated at multiple levels, including transcription, mRNA stability, translation, and protein turnover (16, 25). Transcriptional regulation of the cyclin D1 promoter is driven by several cis-acting elements that are controlled by signaling pathways regulated by cell-extracellular matrix and cell-cell adhesion and involves integrin-linked kinase, focal adhesion kinase, and ß-catenin (5, 10, 17, 60, 63, 75, 76). We identified a novel regulatory binding site in the cyclin D1 promoter, an inverted CCAAT sequence that is essential for the stimulation of transcription by ZONAB. Thus, the regulation of cyclin D1 expression by ZONAB suggests that TJs can cross talk with cell-extracellular matrix and cell-cell adhesion pathways to regulate cell proliferation.

Cyclin D1 activates CDK4 and CDK6 and thereby promotes G1/S-phase transition. ZONAB also binds CDK4, suggesting that the nuclear translocation of the ZONAB/CDK4 complex results in the up-regulation of cyclin D1 expression and, hence, further stimulation of the kinase. Cyclin D1 is also involved in novel functions, such as histone acetylation and chromatin remodeling, cellular metabolism, fat cell differentiation, and cellular migration (25). Because other up-regulated genes that were identified in the cDNA array analysis encode chromatin binding proteins and enzymes involved in energy metabolism, the regulation of cyclin D1 expression by ZONAB may contribute to one of these new cyclin D1 functions.

The increased expression of Y-box binding proteins in somatic cells is associated with cell proliferation and transformation. DbpA, the human homologue of ZONAB, was suggested to be a cellular recombinogenic protein that leads to genomic instability and inflammation-mediated hepatocarcinogenesis, and mutations in the DbpA promoter have been proposed to accelerate hepatocarcinogenesis (29). Moreover, DbpA is regulated by E2F1, suggesting that one of the effects of E2F1 on cell proliferation is the up-regulation of the Y-box factor (6). Similarly, the Y-box factor DbpB/YB-1, which does not localize to TJs, is also up-regulated in many cancers (23, 28). YB-1 binds preferentially to cisplatin-modified DNA and interacts with the PCNA protein (31), suggesting that different Y-box factors regulate PCNA expression and function. The overexpression of ZONAB or down-regulation of ZO-1 decreases the differentiation potential of MDCK cells in 3-D cultures and results in structures with a transformed appearance. On the other hand, the down-regulation of ZONAB significantly decreases cell proliferation, which also interferes with the formation of cysts in 3-D cultures. Thus, the levels of expression of ZONAB and its activation must be tightly regulated during epithelial differentiation. Although the target genes identified now (erbB-2, cyclin D1, and PCNA genes) might be sufficient to explain this phenotype, the cDNA array data suggest that a larger group of genes might be regulated by ZONAB during epithelial differentiation and, possibly, transformation.

By regulating the transcription of the PCNA and cyclin D1 genes, ZONAB regulates the expression of two proteins that are known to be functionally associated. Oncogenesis and chemical carcinogenesis disrupt the stoichiometry of different cyclins, CDK, PCNA, and p21 complexes that are needed for normal linkage of the cell cycle to DNA replication and repair (27, 44, 72, 73). Cyclin D1 is thought to regulate the function of PCNA in DNA repair and replication (52). Thus, the up-regulation of PCNA and cyclin D1 by ZONAB suggests that TJs might play a role in the coordination of the cell cycle with DNA replication and repair.

ZO-1 and ZONAB regulate cell proliferation by at least two mechanisms, sequestration of CDK4 (9) and, as we show here, transcriptional regulation of PCNA and cyclin D1. ZONAB and ZO-1 are up-regulated by serum (11) and DbpA/ZONAB is transcriptionally up-regulated by E2F1 (6). Thus, the target genes of ZONAB that are identified here integrate this transcription factor into a pathway by which well-known regulators of proliferation can stimulate the expression of genes that are required for cell cycle progression. The G1 phase is central to the integration of signals that regulate exit from the cell division cycle to differentiation and the reactivation of cell proliferation; factors involved in these pathways play a role in regulating cell size and number and organogenesis. The most studied regulatory networks of the G1 phase are the retinoblastoma/E2F pathway and the complexes initiating DNA replication. ZONAB regulates both networks, the retinoblastoma/E2F pathway by transcriptional regulation of cyclin D1 and modulation of the nuclear accumulation of CDK4 and the initiation of DNA replication by transcriptional regulation of replication factors, such as PCNA.

As DbpA/ZONAB up-regulation has been observed in carcinomas of the liver and the pancreas (29, 50), two types of cancers that are difficult to treat, it will be important to study in more detail the role of DbpA/ZONAB in tissue biogenesis and maintenance as well as the molecular mechanisms of ZONAB regulation to identify molecular targets and strategies that could be used for pharmacological intervention in cancer cells.


arrow
ACKNOWLEDGMENTS
 
We thank Micheal B. Mathews for PCNA promoter plasmids and the members of our laboratories for discussions. We thank Parminder Mann for her work during the early stage of this project.

This research was supported by the Wellcome Trust (063661 and 066100), MRC, BBSRC and Fight for Sight. A.G. was supported by the European Union (Integrated Project EVI-GENORET: LSHG-CT-2005-512036).


arrow
FOOTNOTES
 
* Corresponding author. Mailing address: Division of Cell Biology, Institute of Ophthalmology, University College London, Bath Street, London EC1V 9EL, United Kingdom. Phone: 0044 20 7608 6861/4014. Fax: 0044 20 7608 4034. E-mail for Maria S. Balda: m.balda{at}ucl.ac.uk. E-mail for Karl Matter: k.matter{at}ucl.ac.uk. Back

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/. Back


arrow
REFERENCES
 
    1
  1. Aijaz, S., M. S. Balda, and K. Matter. Tight junctions: molecular architecture and function. Int. Rev. Cytol., in press.
    2. 2
  2. Aijaz, S., F. D'Atri, S. Citi, M. S. Balda, and K. Matter. 2005. Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition. Dev. Cell 8:777-786.[CrossRef][Medline]
    3. 3
  3. Albanese, C., M. D'Amico, A. T. Reutens, M. Fu, G. Watanabe, R. J. Lee, R. N. Kitsis, B. Henglein, M. Avantaggiati, K. Somasundaram, B. Thimmapaya, and R. G. Pestell. 1999. Activation of the cyclin D1 gene by the E1A-associated protein p300 through AP-1 inhibits cellular apoptosis. J. Biol. Chem. 274:34186-34195.[Abstract/Free Full Text]
    4. 4
  4. Albanese, C., J. Johnson, G. Watanabe, N. Eklund, D. Vu, A. Arnold, and R. G. Pestell. 1995. Transforming p21ras mutants and c-Ets-2 activate the cyclin D1 promoter through distinguishable regions. J. Biol. Chem. 270:23589-23597.[Abstract/Free Full Text]
    5. 5
  5. Amanatullah, D. F., B. T. Zafonte, C. Albanese, M. Fu, C. Messiers, J. Hassell, and R. G. Pestell. 2001. Ras regulation of cyclin D1 promoter. Methods Enzymol. 333:116-127.[Medline]
    6. 6
  6. Arakawa, Y., K. Kajino, S. Kano, H. Tobita, J. Hayashi, M. Yasen, M. Moriyama, and O. Hino. 2004. Transcription of dbpA, a Y box binding protein, is positively regulated by E2F1: implications in hepatocarcinogenesis. Biochem. Biophys. Res. Commun. 322:297-302.[CrossRef][Medline]
    7. 7
  7. Bainbridge, J. W., C. Stephens, K. Parsley, C. Demaison, A. Halfyard, A. J. Thrasher, and R. R. Ali. 2001. In vivo gene transfer to the mouse eye using an HIV-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium. Gene Ther. 8:1665-1668.[CrossRef][Medline]
    8. 8
  8. Balda, M. S., J. M. Anderson, and K. Matter. 1996. The SH3 domain of the tight junction protein ZO-1 binds to a serine protein kinase that phosphorylates a region C-terminal to this domain. FEBS Lett. 399:326-332.[CrossRef][Medline]
    9. 9
  9. Balda, M. S., M. D. Garrett, and K. Matter. 2003. The ZO-1-associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density. J. Cell Biol. 160:423-432.[Abstract/Free Full Text]
    10. 10
  10. Balda, M. S., and K. Matter. 2003. Epithelial cell adhesion and the regulation of gene expression. Trends Cell Biol. 13:310-318.[CrossRef][Medline]
    11. 11
  11. Balda, M. S., and K. Matter. 2000. The tight junction protein ZO-1 and an interacting transcription factor regulate ErbB-2 expression. EMBO J. 19:2024-2033.[CrossRef][Medline]
    12. 12
  12. Bazzoni, G., and E. Dejana. 2004. Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis. Physiol. Rev. 84:869-901.[Abstract/Free Full Text]
    13. 13
  13. Benais-Pont, G., A. Punn, C. Flores-Maldonado, J. Eckert, G. Raposo, T. P. Fleming, M. Cereijido, M. S. Balda, and K. Matter. 2003. Identification of a tight junction-associated guanine nucleotide exchange factor that activates Rho and regulates paracellular permeability. J. Cell Biol. 160:729-740.[Abstract/Free Full Text]
    14. 14
  14. Cereijido, M., L. Shoshani, and R. G. Contreras. 2000. Molecular physiology and pathophysiology of tight junctions. I. Biogenesis of tight junctions and epithelial polarity. Am. J. Physiol. Gastrointest. Liver Physiol. 279:G477-G482.
    15. 15
  15. Chang, C. D., L. Ottavio, S. Travali, K. E. Lipson, and R. Baserga. 1990. Transcriptional and posttranscriptional regulation of the proliferating cell nuclear antigen gene. Mol. Cell. Biol. 10:3289-3296.[Abstract/Free Full Text]
    16. 16
  16. Coqueret, O. 2002. Linking cyclins to transcriptional control. Gene 299:35-55.[CrossRef][Medline]
    17. 17
  17. D'Amico, M., J. Hulit, D. F. Amanatullah, B. T. Zafonte, C. Albanese, B. Bouzahzah, M. Fu, L. H. Augenlicht, L. A. Donehower, K. Takemaru, R. T. Moon, R. Davis, M. P. Lisanti, M. Shtutman, J. Zhurinsky, A. Ben-Ze'ev, A. A. Troussard, S. Dedhar, and R. G. Pestell. 2000. The integrin-linked kinase regulates the cyclin D1 gene through glycogen synthase kinase 3ß and cAMP-responsive element-binding protein-dependent pathways. J. Biol. Chem. 275:32649-32657.[Abstract/Free Full Text]
    18. 18
  18. Daro, E., P. van der Sluijs, T. Galli, and I. Mellman. 1996. Rab4 and cellubrevin define different early endosome populations on the pathway of transferrin receptor recycling. Proc. Natl. Acad. Sci. USA 93:9559-9564.[Abstract/Free Full Text]
    19. 19
  19. Debnath, J., K. R. Mills, N. L. Collins, M. J. Reginato, S. K. Muthuswamy, and J. S. Brugge. 2002. The role of apoptosis in creating and maintaining luminal space within normal and oncogene-expressing mammary acini. Cell 111:29-40.[CrossRef][Medline]
    20. 20
  20. Debnath, J., S. K. Muthuswamy, and J. S. Brugge. 2003. Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures. Methods 30:256-268.[CrossRef][Medline]
    21. 21
  21. Dunn, K. C., A. E. Aotaki-Keen, F. R. Putkey, and L. M. Hjelmeland. 1996. ARPE-19, a human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62:155-169.[CrossRef][Medline]
    22. 22
  22. Fogg, V. C., C. J. Liu, and B. Margolis. 2005. Multiple regions of Crumbs3 are required for tight junction formation in MCF10A cells. J. Cell Sci. 118:2859-2869.[Abstract/Free Full Text]
    23. 23
  23. Fogt, F., C. Poremba, K. Shibao, H. Itoh, K. Kohno, R. L. Zimmerman, H. G. Gortz, B. Dockhorn-Dworniczak, S. J. Urbanski, N. Alsaigh, D. Heinz, A. E. Noffsinger, and K. R. Shroyer. 2001. Expression of survivin, YB-1, and KI-67 in sporadic adenomas and dysplasia-associated lesions or masses in ulcerative colitis. Appl. Immunohistochem. Mol. Morphol. 9:143-149.[CrossRef][Medline]
    24. 24
  24. Frankel, P., A. Aronheim, E. Kavanagh, M. S. Balda, K. Matter, T. D. Bunney, and C. J. Marshall. 2005. RalA interacts with ZONAB in a cell density-dependent manner and regulates its transcriptional activity. EMBO J. 24:54-62.[CrossRef][Medline]
    25. 25
  25. Fu, M., C. Wang, Z. Li, T. Sakamaki, and R. G. Pestell. 2004. Minireview: cyclin D1: normal and abnormal functions. Endocrinology 145:5439-5447.[Abstract/Free Full Text]
    26. 26
  26. Gallia, G. L., E. M. Johnson, and K. Khalili. 2000. Puralpha: a multifunctional single-stranded DNA- and RNA-binding protein. Nucleic Acids Res. 28:3197-3205.[Abstract/Free Full Text]
    27. 27
  27. Gonzales, A. J., T. L. Goldsworthy, and T. R. Fox. 1998. Chemical transformation of mouse liver cells results in altered cyclin D-CDK protein complexes. Carcinogenesis 19:1093-1102.[Abstract/Free Full Text]
    28. 28
  28. Gu, C., T. Oyama, T. Osaki, K. Kohno, and K. Yasumoto. 2001. Expression of Y box-binding protein-1 correlates with DNA topoisomerase II{alpha} and proliferating cell nuclear antigen expression in lung cancer. Anticancer Res. 21:2357-2362.[Medline]
    29. 29
  29. Hayashi, J., K. Kajino, T. Umeda, S. Takano, Y. Arakawa, M. Kudo, and O. Hino. 2002. Somatic mutation and SNP in the promoter of dbpA and human hepatocarcinogenesis. Int. J. Oncol. 21:847-850.[Medline]
    30. 30
  30. Hinz, M., D. Krappmann, A. Eichten, A. Heder, C. Scheidereit, and M. Strauss. 1999. NF-{kappa}B function in growth control: regulation of cyclin D1 expression and G0/G1-to-S-phase transition. Mol. Cell. Biol. 19:2690-2698.[Abstract/Free Full Text]
    31. 31
  31. Ise, T., G. Nagatani, T. Imamura, K. Kato, H. Takano, M. Nomoto, H. Izumi, H. Ohmori, T. Okamoto, T. Ohga, T. Uchiumi, M. Kuwano, and K. Kohno. 1999. Transcription factor Y-box binding protein 1 binds preferentially to cisplatin-modified DNA and interacts with proliferating cell nuclear antigen. Cancer Res. 59:342-346.[Abstract/Free Full Text]
    32. 32
  32. Janda, E., G. Litos, S. Grunert, J. Downward, and H. Beug. 2002. Oncogenic Ras/Her-2 mediate hyperproliferation of polarized epithelial cells in 3-D cultures and rapid tumor growth via the PI3K pathway. Oncogene 21:5148-5159.[CrossRef][Medline]
    33. 33
  33. Joyce, D., C. Albanese, J. Steer, M. Fu, B. Bouzahzah, and R. G. Pestell. 2001. NF-{kappa}B and cell-cycle regulation: the cyclin connection. Cytokine Growth Factor Rev. 12:73-90.[CrossRef][Medline]
    34. 34
  34. Kannabiran, C., G. F. Morris, and M. B. Mathews. 1999. Dual action of the adenovirus E1A 243R oncoprotein on the human proliferating cell nuclear antigen promoter: repression of transcriptional activation by p53. Oncogene 18:7825-7833.[CrossRef][Medline]
    35. 35
  35. Kohno, K., H. Izumi, T. Uchiumi, M. Ashizuka, and M. Kuwano. 2003. The pleiotropic functions of the Y-box-binding protein, YB-1. Bioessays 25:691-698.[CrossRef][Medline]
    36. 36
  36. Kreis, T. E. 1987. Microtubules containing detyrosinated tubulin are less dynamic. EMBO J. 6:2597-2606.[Medline]
    37. 37
  37. Labrie, C., B. H. Lee, and M. B. Mathews. 1995. Transcription factors RFX1/EF-C and ATF-1 associate with the adenovirus E1A-responsive element of the human proliferating cell nuclear antigen promoter. Nucleic Acids Res. 23:3732-3741.[Abstract/Free Full Text]
    38. 38
  38. Lee, B. H., M. Liu, and M. B. Mathews. 1998. Regulation of the human proliferating cell nuclear antigen promoter by the adenovirus E1A-associated protein p107. J. Virol. 72:1138-1145.[Abstract/Free Full Text]
    39. 39
  39. Lee, H. H., W. H. Chiang, S. H. Chiang, Y. C. Liu, J. Hwang, and S. Y. Ng. 1995. Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle. Gene Expr. 4:95-109.[Medline]
    40. 40
  40. Lee, R. J., C. Albanese, R. J. Stenger, G. Watanabe, G. Inghirami, G. K. Haines, III, M. Webster, W. J. Muller, J. S. Brugge, R. J. Davis, and R. G. Pestell. 1999. pp60v-src induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast cancer cells. J. Biol. Chem. 274:7341-7350.[Abstract/Free Full Text]
    41. 41
  41. Li, D., and R. J. Mrsny. 2000. Oncogenic Raf-1 disrupts epithelial tight junctions via downregulation of occludin. J. Cell Biol. 148:791-800.[Abstract/Free Full Text]
    42. 42
  42. Li, Y. Y., L. Wang, and C. D. Lu. 2003. An E2F site in the 5'-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene. FEBS Lett. 544:112-118.[CrossRef][Medline]
    43. 43
  43. Liu, M., B. H. Lee, and M. B. Mathews. 1999. Involvement of RFX1 protein in the regulation of the human proliferating cell nuclear antigen promoter. J. Biol. Chem. 274:15433-15439.[Abstract/Free Full Text]
    44. 44
  44. Loor, G., S. J. Zhang, P. Zhang, N. L. Toomey, and M. Y. Lee. 1997. Identification of DNA replication and cell cycle proteins that interact with PCNA. Nucleic Acids Res. 25:5041-5046.[Abstract/Free Full Text]
    45. 45
  45. Maga, G., and U. Hubscher. 2003. Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J. Cell Sci. 116:3051-3060.[Abstract/Free Full Text]
    46. 46
  46. Matter, K., and M. S. Balda. 2003. Signalling to and from tight junctions. Nat. Rev. Mol. Cell. Biol. 4:225-236.[CrossRef][Medline]
    47. 47
  47. Montesano, R., G. Schaller, and L. Orci. 1991. Induction of epithelial tubular morphogenesis in vitro by fibroblast-derived soluble factors. Cell 66:697-711.[CrossRef][Medline]
    48. 48
  48. Morris, G. F., C. Labrie, and M. B. Mathews. 1994. Modulation of transcriptional activation of the proliferating cell nuclear antigen promoter by the adenovirus E1A 243-residue oncoprotein depends on proximal activators. Mol. Cell. Biol. 14:543-553.[Abstract/Free Full Text]
    49. 49
  49. Nagata, D., E. Suzuki, H. Nishimatsu, H. Satonaka, A. Goto, M. Omata, and Y. Hirata. 2001. Transcriptional activation of the cyclin D1 gene is mediated by multiple cis-elements, including SP1 sites and a cAMP-responsive element in vascular endothelial cells. J. Biol. Chem. 276:662-669.[Abstract/Free Full Text]
    50. 50
  50. Nakatsura, T., S. Senju, K. Yamada, T. Jotsuka, M. Ogawa, and Y. Nishimura. 2001. Gene cloning of immunogenic antigens overexpressed in pancreatic cancer. Biochem. Biophys. Res. Commun. 281:936-944.[CrossRef][Medline]
    51. 51
  51. Noya, F., W. M. Chien, X. Wu, N. S. Banerjee, J. C. Kappes, T. R. Broker, and L. T. Chow. 2002. The promoter of the human proliferating cell nuclear antigen gene is not sufficient for cell cycle-dependent regulation in organotypic cultures of keratinocytes. J. Biol. Chem. 277:17271-17280.[Abstract/Free Full Text]
    52. 52
  52. Pagano, M., A. M. Theodoras, S. W. Tam, and G. F. Draetta. 1994. Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts. Genes Dev. 8:1627-1639.[Abstract/Free Full Text]
    53. 53
  53. Paunesku, T., S. Mittal, M. Protic, J. Oryhon, S. V. Korolev, A. Joachimiak, and G. E. Woloschak. 2001. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int. J. Radiat. Biol. 77:1007-1021.[CrossRef][Medline]
    54. 54
  54. Pollack, A. L., R. B. Runyan, and K. E. Mostov. 1998. Morphogenetic mechanisms of epithelial tubulogenesis: MDCK cell polarity is transiently rearranged without loss of cell-cell contact during scatter factor/hepatocyte growth factor-induced tubulogenesis. Dev. Biol. 204:64-79.[CrossRef][Medline]
    55. 55
  55. Roh, M. H., S. Fan, C. J. Liu, and B. Margolis. 2003. The Crumbs3-Pals1 complex participates in the establishment of polarity in mammalian epithelial cells. J. Cell Sci. 116:2895-2906.[Abstract/Free Full Text]
    56. 56
  56. Sakura, H., T. Maekawa, F. Imamoto, K. Yasuda, and S. Ishii. 1988. Two human genes isolated by a novel method encode DNA-binding proteins containing a common region of homology. Gene 73:499-507.[CrossRef][Medline]
    57. 57
  57. Schneeberger, E. E., and R. D. Lynch. 2004. The tight junction: a multifunctional complex. Am. J. Physiol. Cell Physiol. 286:C1213-C1228.[Abstract/Free Full Text]
    58. 58
  58. Shin, K., S. Straight, and B. Margolis. 2005. PATJ regulates tight junction formation and polarity in mammalian epithelial cells. J. Cell Biol. 168:705-711.[Abstract/Free Full Text]
    59. 59
  59. Shivakumar, C. V., D. R. Brown, S. Deb, and S. P. Deb. 1995. Wild-type human p53 transactivates the human proliferating cell nuclear antigen promoter. Mol. Cell. Biol. 15:6785-6793.[Abstract]
    60. 60
  60. Shtutman, M., J. Zhurinsky, I. Simcha, C. Albanese, M. D'Amico, R. Pestell, and A. Ben-Ze'ev. 1999. The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway. Proc. Natl. Acad. Sci. USA 96:5522-5527.[Abstract/Free Full Text]
    61. 61
  61. Soule, H. D., T. M. Maloney, S. R. Wolman, W. D. Peterson, Jr., R. Brenz, C. M. McGrath, J. Russo, R. J. Pauley, R. F. Jones, and S. C. Brooks. 1990. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50:6075-6086.[Abstract/Free Full Text]
    62. 62
  62. Stampfer, M. R., and P. Yaswen. 2000. Culture models of human mammary epithelial cell transformation. J. Mammary Gland Biol. Neoplasia 5:365-378.[CrossRef][Medline]
    63. 63
  63. Tetsu, O., and F. McCormick. 1999. Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells. Nature 398:422-426.[CrossRef][Medline]
    64. 64
  64. Tschernutter, M., F. C. Schlichtenbrede, S. Howe, K. S. Balaggan, P. M. Munro, J. W. Bainbridge, A. J. Thrasher, A. J. Smith, and R. R. Ali. 2005. Long-term preservation of retinal function in the RCS rat model of retinitis pigmentosa following lentivirus-mediated gene therapy. Gene Ther. 12:694-701.[CrossRef][Medline]
    65. 65
  65. Tsukita, S., M. Furuse, and M. Itoh. 2001. Multifunctional strands in tight junctions. Nat. Rev. Mol. Cell. Biol. 2:285-293.[CrossRef][Medline]
    66. 66
  66. Umeda, K., T. Matsui, M. Nakayama, K. Furuse, H. Sasaki, M. Furuse, and S. Tsukita. 2004. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1. J. Biol. Chem. 279:44785-44794.[Abstract/Free Full Text]
    67. 67
  67. Van Itallie, C. M., and J. M. Anderson. 2004. The molecular physiology of tight junction pores. Physiology 19:331-338.[Abstract/Free Full Text]
    68. 68
  68. Watanabe, G., C. Albanese, R. J. Lee, A. Reutens, G. Vairo, B. Henglein, and R. G. Pestell. 1998. Inhibition of cyclin D1 kinase activity is associated with E2F-mediated inhibition of cyclin D1 promoter activity through E2F and Sp1. Mol. Cell. Biol. 18:3212-3222.[Abstract/Free Full Text]
    69. 69
  69. Watanabe, G., A. Howe, R. J. Lee, C. Albanese, I. W. Shu, A. N. Karnezis, L. Zon, J. Kyriakis, K. Rundell, and R. G. Pestell. 1996. Induction of cyclin D1 by simian virus 40 small tumor antigen. Proc. Natl. Acad. Sci. USA 93:12861-12866.[Abstract/Free Full Text]
    70. 70
  70. Weinmann, A. S., and P. J. Farnham. 2002. Identification of unknown target genes of human transcription factors using chromatin immunoprecipitation. Methods 26:37-47.[CrossRef][Medline]
    71. 71
  71. Williams, M. J., and P. Clark. 2003. Microscopic analysis of the cellular events during scatter factor/hepatocyte growth factor-induced epithelial tubulogenesis. J. Anat. 203:483-503.[CrossRef][Medline]
    72. 72
  72. Xiong, Y., H. Zhang, and D. Beach. 1992. D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA. Cell 71:505-514.[CrossRef][Medline]
    73. 73
  73. Xiong, Y., H. Zhang, and D. Beach. 1993. Subunit rearrangement of the cyclin-dependent kinases is associated with cellular transformation. Genes Dev. 7:1572-1583.[Abstract/Free Full Text]
    74. 74
  74. Zegers, M. M., L. E. O'Brien, W. Yu, A. Datta, and K. E. Mostov. 2003. Epithelial polarity and tubulogenesis in vitro. Trends Cell Biol. 13:169-176.[CrossRef][Medline]
    75. 75
  75. Zhao, J., Z. C. Bian, K. Yee, B. P. Chen, S. Chien, and J. L. Guan. 2003. Identification of transcription factor KLF8 as a downstream target of focal adhesion kinase in its regulation of cyclin D1 and cell cycle progression. Mol. Cell. 11:1503-1515.[CrossRef][Medline]
    76. 76
  76. Zhao, J., R. Pestell, and J. L. Guan. 2001. Transcriptional activation of cyclin D1 promoter by FAK contributes to cell cycle progression. Mol. Biol. Cell 12:4066-4077.[Abstract/Free Full Text]

Molecular and Cellular Biology, March 2006, p. 2387-2398, Vol. 26, No. 6
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.6.2387-2398.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McCrea, P. D., Gu, D., Balda, M. S. (2009). Junctional Music that the Nucleus Hears: Cell-Cell Contact Signaling and the Modulation of Gene Activity. Cold Spring Harb. Perspect. Biol. 1: a002923-a002923 [Abstract] [Full Text]  
  • Schluter, M. A., Margolis, B. (2009). Apical Lumen Formation in Renal Epithelia. J. Am. Soc. Nephrol. 20: 1444-1452 [Abstract] [Full Text]  
  • Tapia, R., Huerta, M., Islas, S., Avila-Flores, A., Lopez-Bayghen, E., Weiske, J., Huber, O., Gonzalez-Mariscal, L. (2009). Zona Occludens-2 Inhibits Cyclin D1 Expression and Cell Proliferation and Exhibits Changes in Localization along the Cell Cycle. Mol. Biol. Cell 20: 1102-1117 [Abstract] [Full Text]  
  • Klein, E. A., Assoian, R. K. (2008). Transcriptional regulation of the cyclin D1 gene at a glance. J. Cell Sci. 121: 3853-3857 [Full Text]  
  • Balda, M. S., Matter, K. (2008). Tight junctions at a glance. J. Cell Sci. 121: 3677-3682 [Full Text]  
  • Berghella, L., De Angelis, L., De Buysscher, T., Mortazavi, A., Biressi, S., Forcales, S. V., Sirabella, D., Cossu, G., Wold, B. J. (2008). A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscle. Genes Dev. 22: 2125-2138 [Abstract] [Full Text]  
  • Darido, C., Buchert, M., Pannequin, J., Bastide, P., Zalzali, H., Mantamadiotis, T., Bourgaux, J.-F., Garambois, V., Jay, P., Blache, P., Joubert, D., Hollande, F. (2008). Defective Claudin-7 Regulation by Tcf-4 and Sox-9 Disrupts the Polarity and Increases the Tumorigenicity of Colorectal Cancer Cells. Cancer Res. 68: 4258-4268 [Abstract] [Full Text]  
  • Srinivasan, K., Roosa, J., Olsen, O., Lee, S.-H., Bredt, D. S., McConnell, S. K. (2008). MALS-3 regulates polarity and early neurogenesis in the developing cerebral cortex. Development 135: 1781-1790 [Abstract] [Full Text]  
  • Huerta, M., Munoz, R., Tapia, R., Soto-Reyes, E., Ramirez, L., Recillas-Targa, F., Gonzalez-Mariscal, L., Lopez-Bayghen, E. (2007). Cyclin D1 Is Transcriptionally Down-Regulated by ZO-2 via an E Box and the Transcription Factor c-Myc. Mol. Biol. Cell 18: 4826-4836 [Abstract] [Full Text]  
  • Pannequin, J., Delaunay, N., Darido, C., Maurice, T., Crespy, P., Frohman, M. A., Balda, M. S., Matter, K., Joubert, D., Bourgaux, J.-F., Bali, J.-P., Hollande, F. (2007). Phosphatidylethanol Accumulation Promotes Intestinal Hyperplasia by Inducing ZONAB-Mediated Cell Density Increase in Response to Chronic Ethanol Exposure. Mol Cancer Res 5: 1147-1157 [Abstract] [Full Text]  
  • Halbleib, J. M., Saaf, A. M., Brown, P. O., Nelson, W. J. (2007). Transcriptional Modulation of Genes Encoding Structural Characteristics of Differentiating Enterocytes During Development of a Polarized Epithelium In Vitro. Mol. Biol. Cell 18: 4261-4278 [Abstract] [Full Text]  
  • Eckert, J. J., Houghton, F. D., Hawkhead, J. A., Balen, A. H., Leese, H. J., Picton, H. M., Cameron, I. T., Fleming, T. P. (2007). Human embryos developing in vitro are susceptible to impaired epithelial junction biogenesis correlating with abnormal metabolic activity. Hum Reprod 22: 2214-2224 [Abstract] [Full Text]  
  • Matter, K., Balda, M. S. (2007). Epithelial tight junctions, gene expression and nucleo-junctional interplay. J. Cell Sci. 120: 1505-1511 [Abstract] [Full Text]  
  • Cannavo, E., Gerrits, B., Marra, G., Schlapbach, R., Jiricny, J. (2007). Characterization of the Interactome of the Human MutL Homologues MLH1, PMS1, and PMS2. J. Biol. Chem. 282: 2976-2986 [Abstract] [Full Text]  
  • Kavanagh, E., Buchert, M., Tsapara, A., Choquet, A., Balda, M. S., Hollande, F., Matter, K. (2006). Functional interaction between the ZO-1-interacting transcription factor ZONAB/DbpA and the RNA processing factor symplekin. J. Cell Sci. 119: 5098-5105 [Abstract] [Full Text]  

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sourisseau, T.
Right arrow Articles by Balda, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sourisseau, T.
Right arrow Articles by Balda, M. S.

Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»