This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sreenivasan, A. Articles by Kellogg, D. R. Search for Related Content PubMed Articles by Sreenivasan, A. Articles by Kellogg, D. R.

 Previous Article

Molecular and Cellular Biology, April 2006, p. 3337, Vol. 26, No. 8
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.8.3337.2006

AUTHOR'S CORRECTION

Specific Inhibition of Elm1 Kinase Activity Reveals Functions Required for Early G₁ Events

Sinsheimer Laboratories, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, California 95064, and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 94143-0450

Volume 23, no. 17, p. 6327-6337, 2003. We have been unable to reproduce a control experiment reported in this paper. Specifically, we found that 25 µM 1-naphthol-methyl PP1 (1NM-PP1) causes a G₁ delay in wild-type cells that is similar to the delay observed in elm1-as cells. While attempting to reproduce this control, we found that addition of supplemental adenine to yeast extract-peptone-dextrose (YPD) growth medium significantly reduces the effects of 1NM-PP1 on wild-type cells. Since our strains are ade^– and grow slowly on YPD medium, we often supplement the media with additional adenine. The addition of adenine to 100 mg/liter largely eliminates the G₁ delay observed in wild-type cells in the presence of 25 µM 1NM-PP1. We also found that the defects in septin organization that we observed before bud emergence were seen only at high concentrations of 1NM-PP1 (25 µM). They may therefore be the combined result of full inhibition of elm1-as and partial inhibition of other kinases. Alternatively, high concentrations of 1NM-PP1 may be necessary to achieve full inhibition of elm1-as. The other results of the paper are unaffected.

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sreenivasan, A. Articles by Kellogg, D. R. Search for Related Content PubMed Articles by Sreenivasan, A. Articles by Kellogg, D. R.