MCB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kiryushko, D.
Right arrow Articles by Bock, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiryushko, D.
Right arrow Articles by Bock, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2006, p. 3625-3638, Vol. 26, No. 9
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.9.3625-3638.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Mechanisms of Ca2+ Signaling in Neurons Induced by the S100A4 Protein

Darya Kiryushko,1,{dagger}* Vera Novitskaya,1,{dagger} Vladislav Soroka,1 Jorg Klingelhofer,2 Eugene Lukanidin,2 Vladimir Berezin,1 and Elisabeth Bock1

Protein Laboratory, Institute of Molecular Pathology, Panum Institute Bld. 6.2, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark,1 Department of Molecular Cancer Biology, Institute of Molecular Cancer Biology, Danish Cancer Society, DK-2100 Copenhagen Ø, Denmark2

Received 17 August 2005/ Returned for modification 13 October 2005/ Accepted 8 February 2006


   ABSTRACT
Top
 Abstract
Introduction
 Materials and Methods
 Results
 Discussion
 References
 
The S100A4 protein belongs to the S100 family of vertebrate-specific proteins possessing both intra- and extracellular functions. In the nervous system, high levels of S100A4 expression are observed at sites of neurogenesis and lesions, suggesting a role of the protein in neuronal plasticity. Extracellular oligomeric S100A4 is a potent promoter of neurite outgrowth and survival from cultured primary neurons; however, the molecular mechanism of this effect has not been established. Here we demonstrate that oligomeric S100A4 increases the intracellular calcium concentration in primary neurons. We present evidence that both S100A4-induced Ca2+ signaling and neurite extension require activation of a cascade including a heterotrimeric G protein(s), phosphoinositide-specific phospholipase C, and diacylglycerol-lipase, resulting in Ca2+ entry via nonselective cation channels and via T- and L-type voltage-gated Ca2+ channels. We demonstrate that S100A4-induced neurite outgrowth is not mediated by the receptor for advanced glycation end products, a known target for other extracellular S100 proteins. However, S100A4-induced signaling depends on interactions with heparan sulfate proteoglycans at the cell surface. Thus, glycosaminoglycans may act as coreceptors of S100 proteins in neurons. This may provide a mechanism by which S100 proteins could locally regulate neuronal plasticity in connection with brain lesions and neurological disorders.


   INTRODUCTION
Top
 Abstract
 Introduction
Materials and Methods
 Results
 Discussion
 References
 
The S100 family is a group of vertebrate-specific Ca2+-binding proteins with a highly conserved primary structure possessing both intra- and extracellular functions. Most S100 family members, including S100A4, are antiparallelly packed homodimers stabilized by disulfide bridges (reviewed in references 8 and 9). Intracellularly, S100 proteins are involved in a variety of processes, including the regulation of cytoskeletal dynamics, Ca2+ homeostasis, and cell proliferation and differentiation. Importantly, some S100 proteins can also be secreted, form oligomers owing to the nonreducing conditions of the environment, and exert their effects acting at the cell surface (10; 43; reviewed in reference 20). A plasma membrane target for S100B and S100A12, the receptor for advanced glycation end products (RAGE), has been identified on inflammatory and neural cells (14). However, RAGE is probably not the sole receptor for members of the S100 family, since the effects of extracellular S100A12 and S100B proteins can be observed in cells lacking RAGE (32), and some of these effects are RAGE independent in cells expressing the receptor (37).

The S100A4 (also termed Mts1) gene was isolated from tumor cells (11, 40), where its expression increased the ability of the tumor to metastasize. S100A4 has also been detected in healthy tissues, particularly in the nervous system. In both the brain and spinal cord, S100A4 expression appears in astrocytes shortly after the start of myelination, with the highest level observed in the areas in which neurogenesis takes place and in regions possessing high plasticity in adults (1). Moreover, in the peripheral nervous system, expression of S100A4 increases after sciatic nerve or dorsal root injury (25). Thus, the release of S100A4 from S100A4-positive astrocytes as a result of either secretion or cell damage might play a role in neuronal plasticity under normal and pathological conditions.

The importance of S100A4 in brain development and/or regeneration is supported by the fact that the protein is a potent promoter of neurite outgrowth in hippocampal neurons in vitro (28). Moreover, S100A4 acts as a neuroprotectant for primary neurons induced to undergo cell death (29). The molecular mechanism of this effect, including a receptor transducing S100A4 signals, has not been identified. However, S100A4-induced neurite outgrowth could be reduced by inhibitors of intracellular Ca2+ homeostasis (28). This indicates that extracellular S100A4 might affect the intracellular Ca2+ concentration ([Ca2+]i), thereby modulating neuronal differentiation.

We show here that the differentiation-promoting oligomeric form of S100A4 increases [Ca2+]i in primary neurons, and we elucidate the molecular mechanism of this effect. We demonstrate that the S100A4-induced neurite outgrowth and [Ca2+]i rise are not mediated by RAGE but depend on an S100A4 interaction with heparan sulfate proteoglycans at the cell surface. Our results suggest that glycosaminoglycans may act as coreceptors of S100 in neurons and/or serve to immobilize S100 proteins in the extracellular matrix. This may provide a mechanism by which S100 proteins could locally regulate neuronal plasticity in connection with brain traumas and neurological disorders.


   MATERIALS AND METHODS
Top
 Abstract
 Introduction
 Materials and Methods
Results
 Discussion
 References
 
Materials. Tetrodotoxin, the inhibitor of voltage-gated sodium channels, the nonselective cation channel inhibitors flufenamic acid and spermine, heparinase I, and Hanks' balanced salt solution (HBSS) were purchased from Sigma-Aldrich A/S (Copenhagen, Denmark). A modified HBSS was produced by adjusting the Ca2+ concentration to 2 mM at pH 7.4. The cyclic AMP (cAMP) antagonist 2',5'-dideoxyadenosine (dd-Ado), the receptor tyrosine kinase inhibitors lavendustin and genistein, the antagonist of Gi/o proteins, pertussis toxin, blockers of voltage-operated calcium channels of the T type (flunarizine), L type (nifedipine), N type ({omega}-conotoxin MVIIA), and P/Q type ({omega}-agatoxin TK), and carbachol were all obtained from Calbiochem (Merck, Darmstadt, Germany). The phospholipase C (PLC) inhibitor U-73122 and the diacylglycerol (DAG)-lipase inhibitor RHC-80267 were purchased from Biomol (Butler Pike, PA). A T-type Ca2+ channel inhibitor, mibefradil (dihydrochloride salt), was a gift from Hoffmann La Roche (Basel, Switzerland). Recombinant S100A4 and S100A12 were cloned and produced as described previously (39).

Primary cultures of rat hippocampal neurons and analysis of neurite outgrowth. Hippocampal and cerebellar neurons were prepared from E19 rat embryos and P3 rat pups as previously described (26, 29, 38). Cultures were seeded at a density of 10,000 cells/cm2 in eight-well LabTek tissue culture chambers with a growth surface of Permanox plastic (Nunc, Roskilde, Denmark) and maintained in Neurobasal medium. Under these conditions, unstimulated cells extended few neurites after 24 h in vitro; however, >90% of the neurons remained alive (unpublished data) and maintained physiological levels of cytoplasmic Ca2+ (unpublished data). Long-term cultures of primary neurons were seeded in eight-well poly-L-lysine-coated LabTek coverglass slides (Nunc) at a density of 50,000 cells/well and grown for 7 to 10 days. Cytosine arabinofuranoside (final concentration, 5 µM; Sigma) was added after 2 days in vitro to inhibit glial cell proliferation. To test whether RAGE was involved in S100 protein-induced neuritogenesis, hippocampal neurons were preincubated with different dilutions of inhibitory antibodies to RAGE and subsequently stimulated with S100A4 and S100A12 (1 µM [each]) or plated on an amphoterin substratum (100 nmol/cm2). The time of preincubation was 1 h for S100A4 and S100A12 and 15 min for the amphoterin substratum. To test the effect of the SA4BP peptide on S100A12- and S100A4-induced neurite outgrowth, recombinant human S100A12 and S100A4 (1 µM [each]) were pretreated with the SA4BP peptide (molar ratios from 1:20 to 1:100) for 1 hour and further applied to the cultures of hippocampal neurons. Analysis of neurite outgrowth was performed as previously described (38).

Fluorometric Ca2+ measurements. Dye loading and experiments were performed in modified HBSS at room temperature (22 to 24°C). Since S100A4 is known to bind Ca2+ with a Kd of ~100 µM (with binding affecting functional properties of the protein), solutions with nominally zero calcium could not be used in the experiments. Instead, low-calcium HBSS was used, which was obtained by decreasing the Ca2+ concentration to 200 µM and adding CdCl2 to the solution to a final concentration of 200 µM. Primary neurons were seeded in eight-well poly-L-lysine-coated LabTek coverglass slides (Nunc) at a density of 50,000 cells/well and grown for 12 to 14 days. Attached cells were loaded with the Ca2+-sensitive dye fura-2 AM (5 µM; Molecular Probes, Eugene, Oreg.) or fluo-4 AM (2 µM; Molecular Probes). To eliminate the effect of synaptically induced Ca2+ fluxes, all experiments were performed in the presence of tetrodotoxin (1 µM). The regions of interest were positioned on somata of the neurons, which were identified by their morphological characteristics. Data were collected from at least three sets of cultures obtained from different animals; each experiment provided simultaneous measurements for up to eight neurons.

Transmembrane potential measurements. The transmembrane potential of neurons was determined using oxonol-6 [diBAC4(3)] (4), a fluorescent membrane potential-sensitive dye (1 µM; Molecular Probes), according to the manufacturer's instructions. Dye loading and experiments were performed in modified HBSS at room temperature (22 to 24°C). Fluorescence intensity traces from individual neurons were obtained by monitoring the average overall intensity of the cell somata (F); a baseline fluorescence level for each cell (F0) was determined by averaging 10 images before the onset of stimulation. Oxonol-6 was excited using a 568-nm line of a 14-mW argon-ion laser; emitted fluorescence was detected using a 600LP filter at 10-s intervals. The [Ca2+]i of each individual neuron was monitored at the same time, using the fluo-4 AM Ca2+-sensitive dye (Molecular Probes) (see below for details).

Microscopy and data analysis. Immunostaining of primary neurons and image scanning were performed as described by Soroka et al. (38). Rabbit anti-rat GAP-43 polyclonal antibodies (Cell Signaling, Beverly, MA) and/or mouse anti-human S100A4 monoclonal antibodies (produced as described in reference 28) were used.

Calcium imaging employing fura-2 AM was performed using a Sensicam 12-bit cooled charge-coupled device camera (PCO, Keilheim, Germany) and a monochromator (J&M, Aalen, Germany) connected to a Zeiss Axiovert 135 TV microscope (Zeiss Fluar x40 oil immersion UV objective, 1.3 numerical aperture). The cellular fluorescence signals were corrected for the background fluorescence, which was measured in a separate region in the vicinity of the cell, by using Imaging Workbench software (Axon, Foster City, CA). Ratio images (340/380-nm excitation; 510-nm LP emission) were collected after background subtraction at 2- to 5-s intervals. The calibration constants and the estimated [Ca2+]i were calculated from a fluorescence ratio, R, as described previously (33).

In experiments employing fluo-4 AM, a confocal laser scanning system (equipped with a Radiance 2000 argon laser [Bio-Rad, NJ]) connected to a Nikon Eclipse TE 200 microscope (Tokyo, Japan) (1.3 numerical aperture, x60 oil immersion objective) and the Lasersharp 2000 software package (Bio-Rad) were used for image acquisition and processing. Fluo-4 was excited using a 488-nm line of a 14-mW argon-ion laser; emitted fluorescence was detected at 510 ± 15 nm at 2-s intervals. The laser light intensity was reduced to 1 to 3% of the nominal intensity by using neutral filters to avoid photodamage of the cells and dye bleaching. Fluorescence intensity traces from individual neurons were obtained and processed as described previously (18).

SPR analysis. Experiments employing surface plasmon resonance (SPR) were performed using a BIAcore-X or BIAcore 2000 instrument (Biacore, Uppsala, Sweden). Two methods of ligand immobilization were used, yielding similar results. In the first method, 3,500 resonance units (RU) of a recombinant human RAGE/Fc chimera (extracellular domain; R&D Systems, Minneapolis, Minn.) was immobilized covalently on a CM5 sensor chip (BIAcore) according to the manufacturer's instructions. Alternatively, 3,500 RU of recombinant RAGE biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (Sigma) was immobilized on an SA sensor chip (BIAcore) with preimmobilized streptavidin. For the analysis of competitive binding of S100A12 and S100A4 to RAGE, 1,200 RU of RAGE was immobilized covalently on a CM5 sensor chip. To study S100A12 binding to S100A4, 1,400 RU of oligomeric S100A4 was immobilized covalently on the sensor chip. Binding of recombinant amphoterin (0.025 to 1.0 µM; R&D Systems), S100A4 (0.2 to 7.3 µM), or S100A12 (0.3 to 9.2 µM) to RAGE and data analysis were performed as described previously (38). Three independent experiments were performed. To analyze binding of heparin to S100 proteins, heparin (1 mg/ml) was biotinylated and immobilized on an SA sensor chip with preimmobilized streptavidin (BIAcore) (see reference 23 for details).

Immunoblotting assay. Hippocampal neurons were seeded in 60-mm tissue culture dishes (106 cells) and grown for 4 h before treatment. Immunoblotting was performed as described previously (24). Rabbit anti-phospho-PLC{gamma}1 antibodies (diluted 1:300; Cell Signaling), rabbit anti-phospho-GAP-43 antibodies (Ser41, diluted 1:1,000; Cell Signaling), or rabbit anti-phospho-ERK1,2 antibodies (Tyr204, diluted 1:1,000; Cell Signaling) were used. Protein bands were visualized using the enhanced chemiluminescence substrate Western Dura (Pierce Biotech, Rockford, IL) and processed with the GenTools software package (Syngene, Cambridge, United Kingdom). To estimate the total amount of PLC{gamma}1, PLC{gamma}2, or GAP-43, membranes were stripped and reprobed with anti-PLC{gamma}1, anti-PLC{gamma}2, or anti-GAP-43 antibodies (all developed in rabbits; Cell Signaling). Immunoblotting for RAGE was performed according to the same protocol, using rabbit anti-RAGE antibodies (diluted 1:400; Santa Cruz, CA).

Statistics and graphical presentation. Statistics and graphical presentations were carried out using the Origin, version 6.0, software package (OriginLab, Northampton, NY). Statistical evaluations were performed using two-sided Student's t test. The results are given as means ± standard errors of the means. Unless stated otherwise, asterisks in figures indicate statistical significance compared to the controls, as follows: *, P < 0.05; **, P < 0.01; and ***, P < 0.001.


   RESULTS
Top
 Abstract
 Introduction
 Materials and Methods
 Results
Discussion
 References
 
Rapid neurite induction by S100A4 involves GAP-43 phosphorylation. It has previously been shown (28) that oligomeric but not dimeric S100A4 induces the differentiation of primary hippocampal neurons. We determined the time-response relationship of the neuritogenic effect of S100A4. Cells stimulated with dimeric S100A4 remained morphologically quiescent even after 4 hours of treatment (Fig. 1A). In contrast, oligomeric S100A4 had already induced lamellipodium formation 5 minutes after application (Fig. 1B). Fifteen minutes after application, the total area of lamellipodia had increased, and solitary filopodia appeared (Fig. 1C). After 4 hours, the formation of primary neurites was observed (Fig. 1D). The induction of neurite outgrowth by S100A4 was associated with phosphorylation of growth-associated protein 43 (GAP-43) by activated protein kinase C (PKC) on a specific site, Ser41, where phosphorylation is known to be essential for neurite elongation in primary neurons (5). In Fig. 1E, it can be seen that S100A4 had already induced phosphorylation of GAP-43 30 min after the start of treatment; the increase in the amount of the phosphorylated form was comparable to that induced by the PKC agonist phorbol 12-myristate 13-acetate and by an activator of PLCß-mediated signaling, carbachol, which activates PKC downstream.


Figure 1
View larger version (50K):
[in this window]
[in a new window]
  		FIG. 1. The fast neuritogenic effect of S100A4 is probably mediated by a cell surface receptor. (A to D) Confocal images of hippocampal neurons treated for the indicated lengths of time with 5 µM dimeric (A) or oligomeric (B to D) S100A4. The cells were immunostained for GAP-43. Representative neurons are shown. Bars, 5 µm. (E) (Top) Increase in GAP-43 phosphorylation induced by carbachol (Cch; 500 nM), phorbol myristate acetate (PMA; 1 µM), and S100A4 (A4; 5 µM). Ctl, control. (Bottom) Results from four independent experiments. (F to H) Hippocampal neurons, grown for 7 days in vitro and not treated (F) or treated (G and H) with 5 µM S100A4 for 10 or 30 min, were coimmunostained for S100A4 (green) and GAP-43 (red). The times of treatment are indicated. Bars, 10 µm.

 
S100A4-induced neurite outgrowth is mediated by a cell surface receptor(s) different from RAGE. Inside the cell, S100A4 is known to bind various target proteins affecting numerous signaling pathways (8). We therefore investigated whether S100A4 enters the cytoplasm after addition to the culture medium. In 7-day in vitro neuronal cultures not treated with S100A4, double immunostaining for GAP-43/S100A4 demonstrated the presence of a few (<0.5% of the total population) S100A4-positive cells that were negative for the neuronal marker GAP-43, probably representing contaminating astrocytes, which are known to express S100A4. Conversely, neurons that were distinctly stained for GAP-43 at the plasma membrane exhibited no trace of S100A4 in the cytoplasm (Fig. 1F). Similarly, no intraneuronal staining for S100A4 was observed in S100A4-treated cultures either 10 min (Fig. 1G) or 30 min (Fig. 1H) after the start of treatment. However, a thin layer or small "blocks" of S100A4 were detected on the surface of the culture dish and on cell membranes, apparently corresponding to extracellularly bound S100A4 (Fig. 1G and H, vertical sections). The intensity of surface staining for S100A4 increased the longer the treatment lasted (compare vertical sections in Fig. 1G and H). Interestingly, the fraction of S100A4-positive cells was significantly higher for S100A4-treated than untreated cultures (2% versus 0.5%, respectively), indicating that some glial cells might take up S100A4 when exposed to the protein.

The same pattern of immunostaining was observed when 6-hour or 7-day in vitro cultures were treated with S100A4 for 5 min, 20 min, 1 h, 4 h, or 24 h (data not shown). Taken together, these data suggest the existence of a plasma membrane target for S100A4. This target rapidly initiates intracellular signaling, resulting in distinct morphological changes within minutes.

To date, only one plasma membrane receptor, RAGE, for S100 proteins (S100B, S100A1, and S100A12) has been identified in neuronal cells (14). RAGE was found to be expressed in cultures of primary hippocampal and cerebellar neurons (Fig. 2A). We therefore examined whether S100A4 is also able to interact directly with RAGE employing SPR analysis. The established RAGE ligands, amphoterin and S100A12, were used as positive controls. As a negative control, we used the S100A2 protein, which in our experiments had no effect on neurite extension from primary hippocampal neurons (not shown). Earlier, radioligand-binding studies showed RAGE to bind amphoterin and S100A12, with Kds of 6.4 ± 1.0 nM and 91 ± 29 nM, respectively (14, 15). In accordance with these observations, we found that both amphoterin and S100A12 interacted with RAGE, with Kds of 6.5 ± 1.9 nM and 79 ± 19 nM, respectively. S100A4 also bound RAGE, although with a lower affinity (Kd = 138 ± 28 nM), whereas binding of S100A2 to RAGE was negligible (Fig. 2B).


Figure 2
View larger version (22K):
[in this window]
[in a new window]
  		FIG. 2. Role of RAGE in S100A4-stimulated neurite outgrowth. (A) Expression of RAGE in primary hippocampal (HN) and cerebellar (CN) neurons, PC12E2 cells, and VMR cells (mouse adenocarcinoma cell line). (B) SPR analysis of binding of immobilized RAGE with recombinant amphoterin, S100A4, S100A12 (1 µM [all]), or S100A2 (10 µM). RU, resonance units. (C) Effect of inhibitory antibodies to RAGE on S100A4-, S100A12-, or amphoterin-stimulated neurite outgrowth. A value of 100% indicates neurite outgrowth from cultures stimulated with S100A12, S100A4, or amphoterin in the absence of antibodies. See Materials and Methods for details. Here and henceforth, "negCTL" indicates neurite outgrowth from nonstimulated cultures. (D) Effect of SA4BP peptide on S100A12- and S100A4-induced neurite outgrowth. A value of 100% indicates neurite outgrowth from neurons stimulated with S100A12 or S100A4 in the absence of the peptide. See Materials and Methods for details. (E) SPR analysis of binding between immobilized RAGE and recombinant S100A4 and S100A12 in the presence and absence of the SA4BP peptide. (F) SPR analysis of binding of immobilized RAGE with recombinant S100A12 (0.29 µM) or with the oligomeric and dimeric forms of S100A4 (both at 0.23 µM). Inset, reduction of the oligomeric form of S100A4 to the dimeric and monomeric forms by dithiothreitol. (G) SPR analysis of competitive binding of immobilized RAGE with recombinant S100A12 (1.6 µM) and S100A4 (2 µM) proteins. (H) SPR analysis of binding of immobilized RAGE with recombinant S100A4 (2 µM) applied either alone or during injection of recombinant S100A12 (2.5 µM). Filled bars indicate the lengths of time for which the proteins were applied. See the text for details.

 
Since RAGE was expressed in primary neurons and interacted with S100A4, we next checked whether RAGE was involved in S100A4-induced neurite outgrowth. However, in our experiments, inhibitory antibodies specific for the extracellular part of RAGE did not affect S100A4-induced neurite outgrowth, whereas they markedly inhibited neurite extension stimulated by either S100A12 or amphoterin (Fig. 2C). Thus, the extracellular part of RAGE seemed to be required for S100A12- but not for S100A4-induced neurite outgrowth.

In order to further characterize the S100A4-induced neurite outgrowth, we used the peptide SA4BP (HSLRSDWVSPNTGGC), identified by a phage display library approach, which specifically bound S100A4, as confirmed by a phage capture assay (J. Klingelhofer et al., unpublished data). When S100A4 or S100A12 was preincubated with SA4BP, the S100A4- but not S100A12-induced neuritogenic response was inhibited in a dose-dependent manner (Fig. 2D). This suggested that the peptide either specifically bound to and blocked the site in S100A4 responsible for the interaction with the receptor transducing the neurite outgrowth signal or changed the conformation of S100A4 so that the protein lost the ability to interact with its receptor. In both cases, if this receptor were RAGE, one would expect that S100A4-RAGE binding would also be affected by SA4BP. To test this, binding of S100A4 or S100A12 to RAGE was compared in the absence and presence of SA4BP (molar ratio of S100 to SA4BP, 1:100 to 1:200). However, no effect of the peptide on S100A4 or S100A12 binding to RAGE was observed (Fig. 2E), indicating that the putative neurite-inducing sites of S100A4 probably do not interact with RAGE.

The dimeric form of S100A4 has been shown to be incapable of inducing cellular responses, even when applied at high concentrations. Therefore, we tested whether reduction of the oligomeric S100A4 protein with dithiothreitol, resulting in the formation of dimers and monomers (Fig. 2F, inset), affected the binding to RAGE. As expected, the resonance response to dimeric/monomeric S100A4 was lower than that to oligomeric S100A4 (Fig. 2F), since the molecular mass of the ligand was decreased. However, the Kd of S100A4 for RAGE was not changed, reflecting similar affinities of reduced and oligomeric S100A4 for RAGE. Thus, the neuritogenically ineffective form of S100A4 was still able to interact with RAGE, further indicating that the neurite-promoting effect of S100A4 is not mediated by RAGE.

It is possible that S100A4 and S100A12 interact with different sites on the RAGE molecule. This might be the reason that the SA4BP peptide and RAGE antibodies have different effects on S100A4- and S100A12-induced neurite outgrowth as well as on the binding of the proteins to RAGE. Therefore, we tested whether S100A4 and S100A12 bind to RAGE in a competitive manner. S100A12 and polymeric S100A4 were applied to a chip with immobilized RAGE either separately (Fig. 2G, A12 and A4) or simultaneously (Fig. 2G, A12+A4). As shown, the resonance response to the simultaneous addition of the proteins did not differ from the response to the S100A12 protein applied alone. This agrees with the observation that the affinity of S100A12 for RAGE is higher than that of S100A4. Thus, upon simultaneous addition of the two proteins, the majority of binding sites of RAGE are expected to be occupied by molecules of S100A12, resulting in association and dissociation dynamics close to those of S100A12 alone. The experiments performed using dimeric/monomeric S100A4 yielded similar results (not shown). In control experiments, S100A12 did not bind S100A4 (not shown). Thus, S100A12 and S100A4 interacted with RAGE competitively, probably sharing the binding site on the RAGE molecule. To confirm this, we tested whether the interaction between S100A4 and immobilized RAGE was affected by prior binding of S100A12 to RAGE. As shown in Fig. 2H, a robust resonance response to S100A4 was observed in the absence of S100A12 (A4ctl). When the same concentration of S100A4 was applied during injection of S100A12 (Fig. 2H, A12+A4), no additional response was detected compared to that induced by injection of S100A12 alone (Fig. 2H, A12ctl). Thus, it can be assumed that S100A12 and S100A4 interacted with the same or significantly overlapping regions of RAGE and that "saturation" of immobilized RAGE by S100A12 decreased the number of binding sites available for subsequent interaction with S100A4.

Oligomeric but not dimeric S100A4 induces a rise of [Ca2+]i involving voltage-dependent Ca2+ channels (VDCC) and affects intracellular Ca2+ stores. The rapid and pronounced morphological changes induced by oligomeric compared to dimeric S100A4 in hippocampal (Fig. 3A and B) and cerebellar (Fig. 3D and E) neurons may be partially accounted for by a change of the cytoplasmic Ca2+ level. Indeed, we found that oligomeric S100A4 elevated [Ca2+]i in hippocampal neurons grown for either 6 h or 7 to 14 days in vitro (Fig. 3C) as well as in cerebellar neurons grown for 3 to 7 days in vitro (Fig. 3F). The Ca2+ response was induced 1 to 2 seconds after protein application, strengthening the contention that S100A4 was acting extracellularly. Interestingly, no Ca2+ response to dimeric S100A4 was observed (Fig. 3C and F), indicating that the S100A4-induced [Ca2+]i rise correlated with the capability for neurite induction of the protein. In addition, the time course of the S100A4-induced Ca2+ responses correlated with that of the S100A4-induced phosphorylation of ERK1 and -2 (Fig. 3G), previously shown to mediate S100A4-induced neurite outgrowth (28). In subsequent experiments, we used S100A4 at a concentration of 15 µM (calculated per monomer) because this dose induced both significant neurite outgrowth (Fig. 3H) and a prominent Ca2+ response (Fig. 3I). As shown in Fig. 3H, cells stimulated with 15 µM S100A4 extended shorter, but more numerous, neurites than those stimulated with 5 µM S100A4. This was in accordance with earlier reports, where significant increases in the average [Ca2+]i of differentiating cells resulted in a higher probability of filopodium formation and a lower rate of neurite outgrowth (reviewed in reference 7). Lower concentrations of S100A4 also increased [Ca2+]i, with a threshold of activation observed at 5 µM S100A4 (Fig. 3I). For Ca2+ fluorometry, hippocampal cultures grown for 7 days in vitro were used because of their robust high-magnitude responses to S100A4. Selected experiments were also reproduced with cultures grown for 6 h in vitro, with similar results (not shown).


Figure 3
View larger version (29K):
[in this window]
[in a new window]
  		FIG. 3. S100A4 increases neurite outgrowth and [Ca2+]i in primary neurons. (A, B, D, E) Neuritogenic response of primary hippocampal (A, B) and cerebellar (D, E) neurons to dimeric (A, D) or oligomeric (B, E) S100A4 at a concentration of 5 µM. (C and F) Ca2+ responses induced by 15 µM S100A4 in primary hippocampal and cerebellar neurons, respectively. (G) Primary hippocampal neurons were treated with 5 µM S100A4 for the indicated lengths of time and further immunoblotted for Tyr204-phosphorylated ERK1 and -2. Representative immunoblots of two to four individual experiments are shown. (H) Neuritogenic responses of primary hippocampal neurons to oligomeric S100A4 at a concentration of 15 µM. (I) Dose-response relationship for S100A4-triggered [Ca2+]i rise in hippocampal neurons. All images represent confocal micrographs of neurons stained for the neuronal marker GAP-43 at 24 h in vitro. In all fluorometric experiments, n = 40 to 60. Bars, 10 µm.

 
Extracellular Ca2+ accounted for the major part of the [Ca2+]i rise after S100A4 application, since the Ca2+ response to S100A4 was abolished by Cd2+, a nonselective antagonist of VDCC (Fig. 4A). To determine which types of VDCC mediated the Ca2+ influx into the cytoplasm, specific blockers were used. Thus, inhibition of L-type VDCC with nifedipine (10 µM) led to a marked (~50%) decrease in the amplitude of the S100A4-evoked [Ca2+]i elevation. A similar effect was obtained with mibefradil, a nonselective inhibitor of T-type Ca2+ channels (Fig. 4A). A more selective T-type VDCC inhibitor, pimozide, abolished the Ca2+ response to S100A4 (Fig. 4A). N-type VDCC, which can be selectively blocked by {omega}-conotoxin, did not play a significant role in mediating the rise of intracellular Ca2+, since the magnitude of the response was the same in the presence of nifedipine and nifedipine plus {omega}-conotoxin (Fig. 4A). The P/Q-type Ca2+ channel inhibitor agatoxin also had no effect (not shown). In agreement with the fluorometric data, S100A4-triggered neurite outgrowth was not affected by agatoxin (not shown) and only slightly affected by high concentrations of {omega}-conotoxin (Fig. 4B), whereas nifedipine and T-type VDCC blockers caused a dose-dependent inhibition of the S100A4-induced neuritogenic response. Reduction in average neurite length at inhibitor doses close to double their K1/2 values was ca. 40% for nifedipine (20 µM) and ca. 90% for mibefradil and pimozide (2.5 µM and 2.2 µM, respectively), thus demonstrating that the activity of T-type but not L-type Ca2+ channels is crucial for neurite induction by S100A4. Interestingly, in PC12E2 cells, which also possess L- and T-type VDCC (6, 19) as well as RAGE (Fig. 2A), no neurite outgrowth or Ca2+ response to S100A4 was observed (not shown). This indicated that S100A4 did not activate the VDCC directly, presumably exerting its effects via direct/indirect modulation of other types of ion channels, and that the mechanism of activation was probably not dependent on RAGE.


Figure 4
View larger version (13K):
[in this window]
[in a new window]
  		FIG. 4. Effects of different blockers of VDCC on S100A4-induced [Ca2+]i rise (A) and neurite outgrowth (B) in primary hippocampal neurons. Ctl, no inhibitor added; Nif, nifedipine (10 µM), a blocker of L-type VDCC; Mib, mibefradil, and Pim, pimozide (5 µM [each]), blockers of T-type VDCC; Ctx, conotoxin (2 µM), a blocker of N-type VDCC; Cd2+ (100 µM), a nonspecific blocker of VDCC. In all fluorometric experiments, n = 20 to 40.

 
Since Ca2+ retained within the endoplasmic reticulum (ER) might also contribute to the S100A4-triggered [Ca2+]i rise, we investigated whether the application of S100A4 evoked a Ca2+ release from the ER stores. Cultured hippocampal neurons have been reported to possess overlapping Ca2+- and inositoltrisphosphate (IP3)-sensitive stores which are functionally empty at rest (17). Nevertheless, raising [Ca2+]i for a period of time enhances Ca2+ uptake into the stores so that more Ca2+ eventually becomes available for release (12). In our experiments, the stores were overcharged by depolarization with 60 mM KCl at an external Ca2+ concentration of 2 mM for 3 min. After being overcharged, neurons acquired the ability to respond to activators of Ca2+- and IP3-induced calcium release (caffeine and carbachol, respectively), whereas no Ca2+ transient could be induced by these agonists before overloading the stores (not shown). To estimate the S100A4-induced Ca2+ release from the ER, the overcharged stores were challenged with S100A4 in a low-Ca2+ medium (see Materials and Methods) ca. 1 min after termination of the charge. A long-lasting, low-amplitude [Ca2+]i transient was observed (Fig. 5A, left panel). After the S100A4-induced response, caffeine was still able to release Ca2+ from the lumen of the ER, suggesting that S100A4 did not empty the caffeine-sensitive stores (Fig. 5A, left panel). When caffeine was applied before S100A4, thereby emptying the stores, the cells were no longer able to mount a Ca2+ response to S100A4 (Fig. 5A, right panel), indicating that S100A4 did indeed induce Ca2+ release from the ER. In the fluorometric experiments, we did not attempt to determine whether S100A4 triggered Ca2+ release via an IP3- or Ca2+-induced mechanism (IICR and CICR, respectively) or both. However, in the neurite outgrowth assay, xestospongin, which blocks IICR, but not ryanodine, which at high concentrations blocks CICR, inhibited S100A4-induced neurite elongation (Fig. 5B). This suggests the involvement of IICR, and thus a phosphoinositide-specific PLC isoform(s), in S100A4-induced intracellular signaling.


Figure 5
View larger version (10K):
[in this window]
[in a new window]
  		FIG. 5. S100A4 triggers Ca2+ release from intracellular stores. (A) The Ca2+ stores of hippocampal neurons were charged with Ca2+, and the cells were challenged with S100A4 either before (left, n = 16) or after (right, n = 7) application of an activator of Ca2+-induced Ca2+ release, i.e., caffeine (caff, 10 mM), in a low-Ca2+ medium. (B) Effects of different blockers of Ca2+ homeostasis in the Ca2+ stores on S100A4-induced neurite outgrowth. Xest, xestospongin, a blocker of IP3 receptors; Rya, ryanodine, a blocker of Ca2+-induced Ca2+ release.

 
S100A4-induced [Ca2+]i rise involves nonselective cation channels and requires activation of the PLC-DAG-DAG-lipase signaling pathway. We next checked whether other types of ion channels besides T- and L-type VDCC were involved in the S100A4-induced [Ca2+]i rise. To test this, the [Ca2+]i and plasma membrane potential of individual cells stimulated with S100A4 were monitored at a physiological or low level of extracellular Ca2+ (Fig. 6A and B, respectively). As shown, oligomeric S100A4 evoked a pronounced [Ca2+]i rise (Fig. 6A) and plasma membrane depolarization (Fig. 6B) in full Ca2+ medium, whereas the dimeric form of the protein failed to affect these parameters. In low-Ca2+ medium, the S100A4-induced Ca2+ responses were inhibited ca. 95% compared to those in full Ca2+ medium (Fig. 6A); however, the S100-triggered change of the membrane potential was only partially decreased (Fig. 6B). This suggested that ion channels permeable for ions other than Ca2+ opened in response to S100A4 and that those might mediate the initial depolarization, subsequently resulting in the opening of T- and L-type VDCC.


Figure 6
View larger version (27K):
[in this window]
[in a new window]
  		FIG. 6. PLC, DAG-lipase, and nonselective cation channels participate in S100A4 signaling. (A and B) Effects of S100A4 on [Ca2+]i (A) and transmembrane potential (B) at physiological and low levels of extracellular Ca2+. (C to E) Effects of two different inhibitors of nonselective cation entry, SKF-96565 and Loe908, on S100A4-evoked [Ca2+]i rise (C and D, respectively) and neurite elongation (E). (F) Effects of a blocker of phosphoinositide-specific PLC, U17352, on S100A4-induced [Ca2+]i elevation. (G and H) Dose-dependent inhibition of S100A4-triggered Ca2+ response (G) and neurite outgrowth (H) by a blocker of DAG-lipase, RHC-80267. In all fluorometric experiments, n = 20 to 40.

 
We thus tested whether nonselective cation channels (NSCC) (reviewed in reference 13) were involved in S100A4-induced signaling. Indeed, two different inhibitors of nonselective Ca2+ entry, Loe-908 and SKF-96562, decreased the S100A4-induced Ca2+ response (Fig. 6C and D), as well as neurite elongation (Fig. 6E), in a dose-dependent manner. In addition, S100A4-induced cellular effects were sensitive to spermine and flufenamic acid, other known inhibitors of NSCC, particularly of transient receptor potential (TRP) family channels (not shown). This indicated that the S100A4-evoked nonselective Ca2+ entry, presumably alongside with entry via T-type VDCC, contributed to the initial plasma membrane depolarization, resulting in the opening of the L-type VDCC and a [Ca2+] rise in the bulk of the cytoplasm.

In many cell types, activation of NSCC is dependent on a phosphoinositide-specific PLC-associated signaling cascade. In this mechanism, the activation of PLC stimulates hydrolysis of phosphatidylinositol 4,5-bisphosphate to IP3 and DAG, which can be further hydrolyzed by DAG-lipase to 2-arachidonoylglycerol (2-AG) and, subsequently, arachidonic acid (AA). All of these messengers activate NSCC (13). We thus checked whether PLC was involved in the cytoplasmic Ca2+ elevation induced by S100A4. Indeed, pretreatment with a low dose (1 µM) of the phosphoinositide-specific PLC inhibitor U-73122 almost completely blocked the Ca2+ response to S100A4 (Fig. 6F). Moreover, blocking DAG-lipase with the specific inhibitor RHC-80267 led to a dose-dependent inhibition of S100A4-triggered Ca2+ transients (Fig. 6G) and neurite outgrowth (Fig. 6H). This ruled out a direct role of DAG in the induction of the S100A4-triggered Ca2+ response and, together with our previous findings, indicated that S100A4-triggered signaling required phosphoinositide-specific PLC and that the hydrolysis of DAG to 2-AG and/or AA was necessary for the induction of the cellular response to S100A4.

Effects of inhibitors of Ca2+ homeostasis on spontaneous neurite outgrowth from primary neurons. To test whether S100A4-induced neurite outgrowth specifically depended on the activation of Ca2+-regulating mechanisms by the protein, we evaluated the effects of inhibitors of these mechanisms on spontaneous neurite outgrowth from primary neurons. To induce spontaneous neurite extension, cells were plated on monolayers of 3T3 fibroblasts, a physiological neuritogenic cue for primary neurons. As can be seen in Fig. 7, blocking of T- and L-type VDCC (pimozide and nifedipine, respectively), NSCC (SKF-96365), and DAG-lipase (RHC-80362) all reduced spontaneous neurite outgrowth. However, the effects of the inhibitors on spontaneous neurite extension were 30 to 50% lower than their effects on S100A4-stimulated neuritogenesis (compare to Fig. 4B and 6E and H). Thus, mechanisms of Ca2+ homeostasis activated by S100A4 might specifically contribute to S100A4-triggered neurite outgrowth.


Figure 7
View larger version (17K):
[in this window]
[in a new window]
  		FIG. 7. Effects of inhibitors of Ca2+ homeostasis on neurite extension from hippocampal neurons grown on top of monolayers of 3T3 fibroblasts for 24 h. A value of 100% indicates neurite outgrowth in the absence of inhibitors. Pim, pimoside; SKF, SKF-90365; Nif, nifedipine; RHC, RHC-80362.

 
S100A4-induced intracellular signaling does not occur via activation of receptor tyrosine kinases (RTKs) or PLC{gamma} but depends on the activation of a heterotrimeric G protein-coupled pathway. The inhibitor of phosphoinositide-specific PLC, U-73122, employed in our study has recently been shown to more potently inhibit PLCß than PLC{gamma} (42), indicating that PLC{gamma} might play a minor role, if any, in the transmission of the S100A4-induced signal. We therefore checked whether either of the two known PLC{gamma} isoforms was activated by S100A4. Since PLC{gamma}2 expression was not detected in hippocampal or cerebellar neurons (Fig. 8A), only the PLC{gamma}1 isoform could, in principle, be activated. Thus, we checked whether PLC{gamma}1 was phosphorylated by S100A4 on the Tyr783 residue, since this phosphorylation is known to be both necessary and sufficient for PLC{gamma}1 activation (21). However, treatment with S100A4 for 5, 30, or 60 min did not result in phosphorylation of PLC{gamma}1, whereas fibroblast growth factor 2 (FGF2), known to activate PLC{gamma}1 via its receptor, caused a significant increase in phosphorylation of the enzyme (Fig. 8B). Thus, PLC{gamma} did not participate in signaling initiated by S100A4.


Figure 8
View larger version (23K):
[in this window]
[in a new window]
  		FIG. 8. S100A4-induced cellular responses do not depend on PLC{gamma}/RTK but probably depend on a G protein-associated signaling cascade. (A) Expression of PLC{gamma}2 in primary hippocampal (HN) and cerebellar (CN) neurons and PC12E2 cells. (B) Primary hippocampal neurons were treated with 5 µM S100A4 or 10 nM FGF2 for the indicated lengths of time and further immunoblotted for Tyr783-phosphorylated PLC{gamma}1. Representative immunoblots of two to four individual experiments are shown. (C and D) Effects of inhibitors of RTKs (genistein and lavendustin A), a nonspecific blocker of G protein-associated signaling (suramin), and a specific blocker of Gq protein-associated signaling (GP-2A) on S100A4-induced neurite extension (C) and Ca2+ responses (D). Lav A, lavendustin A; Sur, suramin; Gq inh, GP-2A. The concentrations of the inhibitors in fluorometric experiments were as follows: lavendustin A, 20 µM; suramin, 100 µM, GP-2A, 10 µM.

 
Receptors capable of activating phosphoinositide-specific PLCs are mainly associated with RTKs or heterotrimeric G proteins (31). A nonspecific antagonist of RTKs, lavendustin A, was not capable of blocking S100A4-induced neurite outgrowth or Ca2+ entry (Fig. 8C and D, respectively). In control experiments, pretreatment with lavendustin A completely prevented the [Ca2+]i rise induced by synthetic ligands of the neural adhesion molecule NCAM, which are known to activate RTK-associated signaling pathways (not shown). Genistein, an inhibitor of a broad range of protein tyrosine kinases, including RTKs, did not have any effect on S100A4-induced neurite outgrowth either (Fig. 8D). However, an inhibitor of G protein-mediated signaling, suramin, completely abrogated the S100A4-triggered neuritogenesis (Fig. 8C) and Ca2+ rise (Fig. 8D). Suramin is not a specific G protein antagonist, since it not only uncouples G proteins from receptors but also inhibits the binding of growth factors at the cell surface (41) and decreases the activity of protein tyrosine phosphatases (PTPs) (27). However, as mentioned above, our data indicated that RTKs were not activated by S100A4. In addition, a broad-range inhibitor of PTP activity, sodium orthovanadate, had only a moderate effect on S100A4-induced neurite outgrowth and no effect on the S100A4-triggered Ca2+ responses (not shown). Thus, even though PTPases might be involved in S100A4-induced neuritogenesis, they seem to have no role in the Ca2+ signaling induced by the protein. Therefore, the blocking effect of suramin on Ca2+ responses could be attributed, at least in part, to the inhibition of G protein activity. Neither the Gi/o- nor Gs-associated signaling pathway participated in S100A4-triggered signaling, since the respective inhibitors of these pathways (pertussis toxin for Gi/o-associated signaling and H-89 or 2',5'-dd-Ado for Gs-adenylate cyclase-PKA-associated signaling [35]) did not affect S100A4-induced cellular responses (not shown). However, both S100A4-induced neurite outgrowth and Ca2+ responses were inhibited by a competitive blocker of G{alpha}q-associated signaling, GP-2A (Fig. 8C and D, respectively). Thus, S100A4 most probably triggered a cascade associated with one or more members of a G{alpha}q/11 class of proteins and a phosphoinositide-specific PLC (presumably PLCß), resulting in Ca2+ entry via NSCC. The activation of G{alpha}q/11 has previously been reported to positively modulate NSCC via PLCß (13) (see Discussion for details).

S100A4- and S100A12-induced neurite outgrowth is partially dependent on interactions with heparan sulfate proteoglycans. The S100A8 and S100A9 proteins have previously been shown to bind heparin and heparan sulfate glycosaminoglycans on the surfaces of endothelial cells (32). We therefore employed SPR analysis to check whether S100A4 and/or S100A12 was also capable of binding heparin. Indeed, both proteins interacted with heparin immobilized on the surface of a sensor chip, with Kds of 53 ± 12 nM and 27 ± 5 nM, respectively (Fig. 9A, lines A4 and A12). The observed binding was specific, since it was inhibited by preincubation of S100A4 and S100A12 with sucrose octosulfate (SOS [a chemical analogue of heparin]) as well as with heparin itself (Fig. 9A).


Figure 9
View larger version (22K):
[in this window]
[in a new window]
  		FIG. 9. S100A4- and S100A12-induced neurite outgrowth is partially dependent on interactions of the two proteins with cell surface heparan sulfates. (A) SPR analysis of binding of immobilized heparin with S100A4 and S100A12 (A4 [0.3 µM] and A12 [0.12 µM], respectively). Preincubation of S100A4 (0.3 µM) and S100A12 (0.12 µM) with 200 µg/ml heparin (+hep) or SOS (+SOS) for 15 min inhibited the binding of the proteins to immobilized heparin. (B) Effect of heparin on S100A4- and S100A12-stimulated neurite outgrowth. CTL (100%), neurite outgrowth from cultures stimulated with S100A12 or S100A4 in the absence of heparin. (C) Dose-dependent inhibition of S100A4-triggered Ca2+ response by heparin (+Hep). S100A4 was preincubated with different concentrations of heparin for 10 min before applying the protein to the cultures. Since the dose of S100A4 in Ca2+ fluorometric experiments was higher than that in the neurite outgrowth assay (15 µM versus 1 µM), the concentrations of heparin were increased accordingly. Hep, 3 mg/ml, Ca2+ response to heparin alone. (D) Effect of treatment with heparinase I on S100A4- and S100A12-stimulated neurite outgrowth. CTL (100%), neurite outgrowth from cultures stimulated with S100A12 or S100A4 in the absence of heparinase I.

 
In accordance with the SPR analysis results, both S100A4- and S100A12-induced neuritogenic responses were significantly lower in the presence of heparin (Fig. 9B). Moreover, the Ca2+ responses to S100A4 were decreased by heparin in a dose-dependent manner (Fig. 9C). Heparin itself did not affect neurite outgrowth (not shown) and had a minor effect on [Ca2+]i at a dose of 3 mg/ml (Fig. 9C). The maximum degree of inhibition of both S100A4-induced neurite outgrowth and Ca2+ responses was ca. 50%. In addition, enzymatic removal of heparan sulfates from the cell surface by treatment with heparinase I significantly reduced both S100A12- and S100A4-induced neurite extension (Fig. 9D), emphasizing the importance of these proteoglycans for S100A12 and S100A4 function. Thus, heparan sulfate moieties on the cell surface and/or in the extracellular matrix may function as coreceptors for both S100A4 and S100A12. In particular, S100A12 seems to require the presence of both heparan sulfates and RAGE to induce neuronal differentiation.


   DISCUSSION
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
References
 
In this study, we have investigated the mechanism underlying the induction of differentiation and a [Ca2+]i rise in primary neurons by S100A4. Interestingly, only the oligomeric form of S100A4 was capable of triggering these responses. Several reasons might account for this. Firstly, the conformation of S100A4 in dimeric form may be different from that of S100A4 in oligomeric form, such that the sites responsible for the binding to its target are not exposed in the dimer. Secondly, S100A4 may activate target proteins by clustering them; in that case, oligomeric S100A4 will be more potent because of a larger number of potential binding sites.

No quantitative data are available on the concentration of S100A4 in the extracellular space under physiological conditions and/or after neural injuries. However, expression of the protein has been demonstrated in a variety of neural cells, and it is strongly increased after brain traumas and nerve lesions (25, 34). Moreover, secretion of S100A4 in vitro has been reported, with the concentration of the secreted protein in the culture medium estimated to be about 10 µM (10), which is comparable with the S100A4 concentrations used in this study. Thus, given the relatively low volume of the extracellular space, physiological secretion of S100A4 and/or its release from damaged neural cells after neuronal injuries might provide sufficiently high local concentrations of S100A4.

The effect of S100A4 seemed to be mediated by a plasma membrane receptor. Several lines of evidence support this suggestion. Firstly, staining for S100A4 was detected only at the cell surfaces of neurons treated with the protein. Secondly, the effect of S100A4 on [Ca2+]i was observed within 2 to 3 seconds, a time span which is too short for the protein to penetrate the plasma membrane via endocytosis or passive diffusion, with the latter being hampered by the large size of the protein (ca. 40 kDa for the tetramer). Thirdly, the induced signaling was abolished by inhibition of PLC, further confining the site of S100A4 action to the membrane. Finally, suramin, known to block the binding of agonists to G protein-associated receptors and RTKs, completely prevented the induction of cellular responses to S100A4.

It has long been known that S100A4, along with other proteins of the S100 family, including S100A1, S100A12, and S100B, serves as a potent glia-derived inducer of neuronal differentiation and as a survival factor. However, the receptor(s) responsible for the S100A4-induced signal transduction has not been identified. A clue came from a study (14) where RAGE was shown to interact with S100A12. The signaling induced by S100A12 was of crucial significance for processes such as chronic inflammation and tissue injury. RAGE was also demonstrated to be implicated in the S100A1- and S100B-induced neurite outgrowth from N18 neuroblastoma cells (16). Thus, S100A4 might also stimulate neurite extension from primary neurons via RAGE. In vitro, we found that S100A4 bound to RAGE competitively with S100A12; however, the affinities of S100A4 and S100A12 for RAGE differed significantly. This might imply that different regions of S100A12 and S100A4 are involved in the interaction with RAGE. Alternatively, S100A12 and S100A4 might bind to sites of the RAGE molecule that are partially overlapping or situated in close proximity. In this case, given the large sizes of the proteins, the S100A12-RAGE and S100A4-RAGE interactions could be competitive but might initiate different signaling events. Accordingly, the SA4BP peptide, which specifically bound to S100A4 and inhibited S100A4-induced neurite outgrowth, did not interfere with S100A4-RAGE binding. In addition, inhibitory antibodies to RAGE were unable to block S100A4-induced neurite outgrowth while significantly inhibiting outgrowth induced by S100A12 and by the RAGE-specific ligand amphoterin (see Results). Finally, the dimeric form of S100A4, which had no effect on neurite outgrowth from primary neurons, bound to RAGE with an affinity similar to that of the oligomeric form. In principle, RAGE-mediated differentiation might require receptor clustering on the cell surface. However, this is not very likely, since in our experiments RAGE-mediated neurite outgrowth was also elicited by S100A12, which was expressed in the dimeric form. Thus, the S100A4-RAGE interaction, though occurring in vitro, is probably not necessary for neurite induction by S100A4. Instead, this interaction may mediate the neuroprotective effect of S100A4 exhibited by both dimeric and oligomeric forms (29).

In our experiments, the threshold concentration of S100A4 inducing an elevation of the bulk [Ca2+]i was ca. 5 µM. At this concentration, a low-amplitude Ca2+ response lasting for ca. 2 min was observed in the somata of neurons. Interestingly, we also found that S100A4 had already activated ERK1 and -2, the kinases crucial for induction of neurite outgrowth by the protein, after 1 min of treatment (Fig. 3G). This indicates a specific role of Ca2+-regulating mechanisms in S100A4-induced signaling. In addition, the genuine threshold of activation of Ca2+ responses might be significantly lower, since low concentrations of S100A4 might evoke a [Ca2+]i increase that is not detectable using conventional Ca2+ fluorometry, as demonstrated for other neurite-inducing cues (2, 22).

The amplitude of the observed Ca2+ response to S100A4 was dependent on how long in vitro a neuronal culture had been grown, probably reflecting the difference in surface density of a putative S100A4 receptor or, more likely, the age-dependent increase in surface density of L-type VDCC (30), which, along with T-type VDCC, are mainly responsible for the depolarization-induced Ca2+ influx into neuronal somata. Accordingly, the S100A4-induced [Ca2+]i response was crucially dependent on the extracellular calcium concentration and could be significantly inhibited by blockers of both L- and T-type VDCC but not other types of VDCC. Since a blockade of T-type VDCC abolished the S100A4-triggered [Ca2+]i rise completely, it seems plausible that low-voltage-activated T-type VDCC open first, resulting in depolarization and a subsequent opening of high-voltage-activated L-type VDCC.

In our experiments, the inhibition of G proteins, but not RTKs or PTPs, abrogated the S100A4-induced Ca2+ responses, suggesting that one or more members of the G protein family were involved. However, only the G{alpha}q-associated signaling pathway was of importance for the neurite outgrowth/[Ca2+]i rise triggered by S100A4. The involvement of a phosphoinositide-specific PLC in S100A4 signaling suggests that S100A4 binding to a receptor on the cell surface might result in activation of the G{alpha}q/11 class proteins associated with PLCß (summarized in Fig. 10). In this event, G{alpha}q/11-activated PLCß mediates the formation of DAG, which is hydrolyzed to form two sequential products, 2-AG and AA, with all three messengers known to increase plasma membrane permeability for Ca2+. In support of this hypothesis, blocking of PLC, DAG-lipase, or NSCC led to an inhibition of the S100A4-evoked neurite outgrowth and [Ca2+]i rise (see Results). These data also indicated that the downstream products of DAG, i.e., 2-AG and AA, were responsible for the induced cellular responses. Recently, intrinsic AA and its metabolites have been shown to activate a noncapacitative Ca2+ entry in a variety of cell types via newly identified arachidonate-regulated Ca2+ channels (reviewed in reference 36) and TRP-family Ca2+-permeable channels, suggesting that these could be the NSCC mediating the S100A4-induced Ca2+ entry (Fig. 10).


Figure 10
View larger version (21K):
[in this window]
[in a new window]
  		FIG. 10. Schematic representation of signaling pathways activated by S100A4 interacting with its putative receptor. S100A4R, a putative S100A4 receptor; HSPG, heparan sulfate proteoglycans; CAMK, calcium-calmodulin kinase; MEK, mitogen-activated protein kinase kinase; CREB, cAMP response element binding protein. See Discussion for details.

 
Interestingly, in control experiments, we found that inhibitors of L- and T-type VDCC, NSCC, or DAG-lipase had less effect on neurite extension from neurons grown on monolayers of 3T3 fibroblasts (Fig. 7) than on S100A4-induced neuritogenesis. This indicated a specific role of Ca2+-regulating mechanisms in the S100A4-induced signaling. However, it cannot be ruled out that several neuritogenic cues might influence [Ca2+]i through the same effectors as S100A4, since primary neurons possess a restricted array of Ca2+-regulating mechanisms.

We also found that heparin specifically bound both S100A4 and S100A12 and influenced the neurite outgrowth and Ca2+ rise induced by these two proteins. Since heparan glycosaminoglycans are abundant both in the extracellular matrix and on cell membranes, they might act as a scaffold to immobilize S100 proteins extracellularly. Due to the high affinity of the S100-heparan sulfate interaction, extracellular S100 proteins would be captured close to the site of their initial release. This might provide a mechanism by which S100 proteins could locally regulate neuronal plasticity in connection with brain traumas and neurological disorders, since the cellular effects of the proteins would be confined to the areas adjacent to the site of damage.

In conclusion, we have shown for the first time that the differentiation-promoting oligomeric form of S100A4 increases the cytoplasmic calcium concentration ([Ca2+]i) in primary neurons. We present evidence that S100A4-induced intracellular signaling involves the activation of a cascade involving a heterotrimeric G protein(s), phosphoinositide-specific PLC, and DAG-lipase, resulting in Ca2+ entry via nonselective cation channels and Ca2+ influx via T- and L-type voltage-gated Ca2+ channels. Moreover, our data indicate that S100A4-induced signaling in primary neurons does not depend on the known target for other extracellular S100 proteins, RAGE, suggesting the existence of other plasma membrane receptors for S100 proteins. To activate these as yet unidentified receptors, S100 proteins might require initial binding to glycosaminoglycans at the cell surface or in the extracellular matrix. Thus, S100 proteins, in particular S100A4, may represent "multireceptor ligands," and their cellular effects may be a combination of outputs of several signaling cascades.


   ACKNOWLEDGMENTS
 
This study was supported by Danish Cancer Society grant DP 04 051.


   FOOTNOTES
 
* Corresponding author. Mailing address: Protein Laboratory, Panum Institute, Blegdamsvej 3C, Bld. 6.2, Copenhagen 2200N, Denmark. Phone: (45) 35 32 73 30. Fax: (45) 35 36 01 16. E-mail: darya{at}plab.ku.dk. Back

{dagger} D. Kiryushko and V. Novitskaya contributed equally to this work. Back


   REFERENCES
Top
 Abstract
 Introduction
 Materials and Methods
 Results
 Discussion
 References
 
1. Åberg, F., and E. Kozlova. 2000. Metastasis-associated Mts1/S100A4 protein in the developing and adult central nervous system. J. Comp. Neurol. 424:269-282.[CrossRef][Medline]

2. Archer, F. R., P. Doherty, D. Collins, and S. R. Bolsover. 1999. CAMs and FGF cause a local submembrane calcium signal promoting axon outgrowth without a rise in bulk calcium concentration. Eur. J. Neurosci. 11:3565-3573.[CrossRef][Medline]

3. Barger, S. W., and L. J. Van Eldik. 1992. S100B stimulates calcium fluxes in glial and neuronal cells. J. Biol. Chem. 14:9689-9694.

4. Brauner, T., D. F. Hulser, and R. J. Strasser. 1984. Comparative measurements of membrane potentials with microelectrodes and voltage-sensitive dyes. Biochim. Biophys. Acta 771:208-216.[Medline]

5. Coggins, P. J., and H. Zwiers. 1989. Evidence for a single protein kinase C-mediated phosphorylation site in rat brain protein B-50. J. Neurochem. 53:1895-1901.[CrossRef][Medline]

6. Del Toro, R., K. L. Levitsky, J. Lopez-Barneo, and M. D. Chiara. 2003. Induction of T-type calcium channel gene expression by chronic hypoxia. J. Biol. Chem. 278:22316-22324.[Abstract/Free Full Text]

7. Dent, E. W., F. Tang, and K. Kalil. 2003. Axon guidance by growth cones and branches: common cytoskeletal and signaling mechanisms. Neuroscientist 9:343-353.[Abstract]

8. Donato, R. 2001. S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles. Int. J. Biochem. Cell. Biol. 33:637-668.[CrossRef][Medline]

9. Donato, R. 2003. Intracellular and extracellular roles of S100 proteins. Microsc. Res. Tech. 60:540-551.[CrossRef][Medline]

10. Duarte, W. R., T. Iimura, K. Takenaga, K. Ohya, I. Ishikawa, and S. Kasugai. 1999. Extracellular role of S100A4 calcium-binding protein in the periodontal ligament. Biochem. Biophys. Res. Commun. 255:416-420.[CrossRef][Medline]

11. Ebralidze, A., E. Tulchinsky, M. Grigorian, A. Afanasyeva, V. Senin, E. Revazova, and E. Lukanidin. 1989. Isolation and characterization of a gene specifically expressed in different metastatic cells and whose deduced gene product has a high degree of homology to a Ca2+-binding protein family. Genes Dev. 3:1086-1093.[Abstract/Free Full Text]

12. Garaschuk, O., Y. Yaari, and A. Konnerth. 1997. Release and sequestration of calcium by ryanodine-sensitive stores in rat hippocampal neurones. J. Physiol. 502:13-30.[CrossRef][Medline]

13. Hardie, R. C. 2003. Regulation of TRP channels via lipid second messengers. Annu. Rev. Physiol. 65:735-759.[CrossRef][Medline]

14. Hofmann, M. A., S. Drury, C. Fu, W. Qu, A. Taguchi, Y. Lu, C. Avila, N. Kambham, A. Bierhaus, P. Nawroth, M. F. Neurath, T. Slattery, D. Beach, J. McClary, M. Nagashima, J. Morser, D. Stern, and A. M. Schmidt. 1999. RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell 97:889-901.[CrossRef][Medline]

15. Hori, O., J. Brett, T. Slattery, R. Cao, J. Zhang, J. X. Chen, M. Nagashima, E. R. Lundh, S. Vijay, and D. Nitecki. 1995. The receptor for advanced glycation end products (RAGE) is a cellular binding site for amphoterin. Mediation of neurite outgrowth and co-expression of RAGE and amphoterin in the developing nervous system. J. Biol. Chem. 270:25752-25761.[Abstract/Free Full Text]

16. Huttunen, H. J., J. Kuja-Panula, G. Sorci, A. L. Agneletti, R. Donato, and H. Rauvala. 2000. Coregulation of neurite outgrowth and cell survival by amphoterin and S100 proteins through RAGE activation. J. Biol. Chem. 275:40096-40105.[Abstract/Free Full Text]

17. Irving, A. J., and G. L. Collingridge. 1998. A characterization of muscarinic receptor-mediated intracellular Ca2+-mobilization in cultured rat hippocampal neurons. J. Physiol. 511:747-759.[Abstract/Free Full Text]

18. Jacobs, M., and T. Meyer. 1997. Control of action potential-induced Ca2+ signalling in the soma of hippocampal neurons by Ca2+ release from intracellular stores. J. Neurosci. 17:4129-4135.[Abstract/Free Full Text]

19. Janigro, D., G. Maccaferri, and J. Meldolesi. 1989. Calcium channels in undifferentiated PC12 rat pheochromocytoma cells. FEBS Lett. 255:398-400.[CrossRef][Medline]

20. Kerkhoff, C., V. Klempt, and C. Sorg. 1998. Novel insights into structure and function of VRP8 S100A8 and MRP14 S100A9. Biochim. Biophys. Acta 1448:200-211.[Medline]

21. Kim, H. K., J. W. Kim, A. Zilberstein, B. Margolis, J. G. Kim, J. Schlessinger, and S. Rhee. 1991. PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254. Cell 65:435-441.[CrossRef][Medline]

22. Kiryushko, D., V. Berezin, and E. Bock. 2004. Regulators of neurite outgrowth. The role of cell adhesion molecules. Ann. N. Y. Acad. Sci. 1014:140-154.[Abstract/Free Full Text]

23. Kiselyov, V. V., V. Berezin, T. E. Maar, V. Soroka, K. Edvardsen, A. Schousboe, and E. Bock. 1997. The first immunoglobulin-like neural cell adhesion molecule (NCAM) domain is involved in double-reciprocal interaction with the second immunoglobulin-like NCAM domain and in heparin binding. J. Biol. Chem. 272:10125-10134.[Abstract/Free Full Text]

24. Kolkova, K., V. Novitskaya, N. Pedersen, V. Berezin, and E. Bock. 2000. NCAM-stimulated neurite outgrowth depends on activation of protein kinase C and the Ras-mitogen activation protein kinase pathway. J. Neurosci. 20:2238-2246.[Abstract/Free Full Text]

25. Kozlova, E. N., and E. Lukanidin. 1999. Metastasis-associated Mts1/S100A4 protein is selectively expressed in white matter astrocytes and is upregulated after peripheral nerve or dorsal root injury. Glia 27:249-258.[CrossRef][Medline]

26. Maar, T. E., L. Rønn, E. Bock, V. Berezin, J. Moran, H. Pasantes-Morales, and A. Schousboe. 1997. Characterization of microwell cultures of dissociated brain tissue for studies of cell-cell interactions. J. Neurosci. Res. 47:163-172.[CrossRef][Medline]

27. McCain, D. F., L. Wu, P. Nickel, M. U. Kassack, A. Kreimeyer, A. Gagliardi, D. C. Collins, and Z. Y. Zhang. 2004. Suramin derivatives as inhibitors and activators of protein-tyrosine phosphatases. J. Biol. Chem. 279:14713-14725.[Abstract/Free Full Text]

28. Novitskaya, V., M. Grigorian, M. Kriajevska, S. Tarabykina, I. Bronstein, V. Berezin, E. Bock, and E. Lukanidin. 2000. Oligomeric forms of the metastasis-related Mts1/S100A4 protein stimulate neuronal differentiation in cultures of rat hippocampal neurons. J. Biol. Chem. 52:41278-41286.

29. Pedersen, M., L. Kohler, M. Grigorian, V. Novitskaya, E. Bock, E. Lukanidin, and V. Berezin. 2004. The Mts1/S100A4 protein is a neuroprotectant. J. Neurosci. Res. 77:777-786.[CrossRef][Medline]

30. Porter, N. M., O. Thibault, V. Thibault, K.-C. Chen, and P. W. Landfield. 1997. Calcium channel density and hippocampal cell death with age in long-term culture. J. Neurosci. 17:5629-5639.[Abstract/Free Full Text]

31. Rebecchi, M. J., and S. N. Pentyala. 2000. Structure, function, and control of phosphoinositide-specific phospholipase C. Phys. Rev. 50:1291-1335.

32. Robinson, M. J., P. Tessier, R. Poulsom, and N. Hogg. 2002. The S100 family heterodimer, MRP-8/14, binds with high affinity to heparin and heparan sulphate glycosaminoglycans on endothelial cells. J. Biol. Chem. 277:3658-3665.[Abstract/Free Full Text]

33. Rønn, L., S. Dissing, A. Holm, V. Berezin, and E. Bock. 2002. Increased intracellular calcium is required for neurite outgrowth induced by a synthetic peptide ligand of NCAM. FEBS Lett. 518:60-66.[CrossRef][Medline]

34. Sandelin, M., S. Zabihi, L. Liu, G. Wicher, and E. Kozlova. 2004. Metastasis-associated S100A4 (Mts1) protein is expressed in subpopulations of sensory and autonomic neurons and in Schwann cells of the adult rat. J. Comp. Neurol. 473:233-243.[CrossRef][Medline]

35. Schmidt, M., S. Evellin, P. Weernink, F. von Dorp, H. Rehmann, J. W. Lomasney, and K. H. Jakobs. 2001. A new phospholipase-C-calcium signalling pathway mediated by cyclic AMP and a Rap-GTPase. Nat. Cell Biol. 3:1020-1025.[CrossRef][Medline]

36. Shuttleworth, T. J., and O. Mignen. 2003. Calcium entry and the control of calcium oscillations. Biochem. Soc. Trans. 31:916-919.[Medline]

37. Sorci, G., F. Riuzzi, C. Marchetti, and R. Donato. 2002. S100B causes apoptosis in myoblasts and inhibits myogenic differentiation and myotube formation in a RAGE-independent manner, abstr. PJ42. Seventh European Symposium on Calcium-Binding Proteins in Normal and Transformed Cells, Brussels, Belgium, 12 to 16 June 2002.

38. Soroka, V., D. Kiryushko, V. Novitskaya, L. Rønn, F. M. Poulsen, A. Holm, E. Bock, and V. Berezin. 2002. Induction of neuronal differentiation by a peptide corresponding to the homophilic binding site of the second Ig module of the neural cell adhesion molecule. J. Biol. Chem. 277:24676-24683.[Abstract/Free Full Text]

39. Tarabykina, S., M. Kriajevska, D. J. Scott, T. J. Hill, D. Lafitte, P. J. Derrick, G. G. Dodson, E. Lukanidin, and I. Bronstein. 2000. Heterocomplex formation between metastasis-related protein S100A4/Mts1 and S100A1 as revealed by the yeast two-hybrid system. FEBS Lett. 475:187-191.[CrossRef][Medline]

40. Vallely, K. M., R. R. Rustandi, K. C. Ellis, O. Varlamova, A. R. Bresnick, and D. J. Weber. 2002. Solution structure of human Mts1 (S100A4) as determined by NMR spectroscopy. Biochemistry 41:12670-12680.[CrossRef][Medline]

41. Voogd, T. E., L. M. Vansterkenburg, J. Wilting, and L. Janssen. 1993. Recent research on the biological activity of suramin. Pharm. Rev. 45:177-203.[Medline]

42. Ward, P. D., R. R. Klein, M. D. Troutman, S. Desai, and D. R. Thakker. 2002. Phospholipase C-{gamma} modulates epithelial tight junction permeability through hyperphosphorylation of tight junction proteins. J. Biol. Chem. 277:35760-35765.